WO2008071715A1 - Herbicide-resistant sunflower plants and methods of use - Google Patents
Herbicide-resistant sunflower plants and methods of use Download PDFInfo
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- WO2008071715A1 WO2008071715A1 PCT/EP2007/063737 EP2007063737W WO2008071715A1 WO 2008071715 A1 WO2008071715 A1 WO 2008071715A1 EP 2007063737 W EP2007063737 W EP 2007063737W WO 2008071715 A1 WO2008071715 A1 WO 2008071715A1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/12—Processes for modifying agronomic input traits, e.g. crop yield
- A01H1/122—Processes for modifying agronomic input traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- A01H1/123—Processes for modifying agronomic input traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
- A01H5/10—Seeds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/50—1,3-Diazoles; Hydrogenated 1,3-diazoles
Definitions
- This invention relates to the field of agricultural biotechnology, particularly to herbicide-resistant sunflower plants and novel polynucleotide sequences that encode wild-type and herbicide-resistant sunflower acetohydroxyacid synthase large subunit proteins.
- Acetohydroxyacid synthase (AHAS; EC 4.1.3.18, also known as acetolactate synthase or ALS), is the first enzyme that catalyzes the biochemical synthesis of the branched chain amino acids valine, leucine and isoleucine (Singh (1999)
- AHAS Biosynthesis of valine, leucine and isoleucine
- Plant Amino Acid Singh, B.K., ed., Marcel Dekker Inc. New York, New York, pp. 227-247.
- AHAS is the site of action of five structurally diverse herbicide families including the sulfonylureas (LaRossa and Falco (1984) Trends Biotechnol. 2:158-161), the imidazolinones (Shaner et al. (1984) Plant Physiol.
- sulfonylurea herbicides are chlorsulfuron, metsulfuron methyl, sulfometuron methyl, chlorimuron ethyl, thifensulfuron methyl, tribenuron methyl, bensulfuron methyl, nicosulfuron, ethametsulfuron methyl, rimsulfuron, triflusulfuron methyl, triasulfuron, primisulfuron methyl, cinosulfuron, amidosulfiuon, fluzasulfuron, imazosulfuron, pyrazosulfuron ethyl and halosulfuron.
- imidazolinone herbicides are favored for application by spraying over the top of a wide area of vegetation.
- the ability to spray a herbicide over the top of a wide range of vegetation decreases the costs associated with plant establishment and maintenance, and decreases the need for site preparation prior to use of such chemicals.
- Spraying over the top of a desired tolerant species also results in the ability to achieve maximum yield potential of the desired species due to the absence of competitive species.
- the ability to use such spray-over techniques is dependent upon the presence of imidazolinone-resistant species of the desired vegetation in the spray over area.
- leguminous species such as soybean are naturally resistant to imidazolinone herbicides due to their ability to rapidly metabolize the herbicide compounds (Shaner and Robinson (1985) Weed Sd. 33:469-471).
- Other crops such as corn (Newhouse et al (1992) Plant Physiol. 100:882-886) and rice (Barrett et al. (1989) Crop Safeners for Herbicides, Academic Press, New York, pp. 195-220) are somewhat susceptible to imidazolinone herbicides.
- the differential sensitivity to the imidazolinone herbicides is dependent on the chemical nature of the particular herbicide and differential metabolism of the compound from a toxic to a non-toxic form in each plant (Shaner et al. (1984) Plant Physiol. 76:545-546; Brown et al, (1987) Pestic. Biochem. Physiol. 27:24-29). Other plant physiological differences such as absorption and translocation also play an important role in sensitivity (Shaner and Robinson (1985) Weed Sd. 33:469-471).
- Plants resistant to imidazolinones, sulfonylureas, triazolopyrimidines, and pyrimidyloxybenzoates have been successfully produced using seed, microspore, pollen, and callus mutagenesis in Zea mays, Arabidopsis thaliana, Brassica napus ⁇ i.e., canola) Glydne max, Nicotiana tabacum, sugarbeet ⁇ Beta vulgaris) and Oryza sativa (Sebastian et al. (1989) Crop Sd. 29:1403-1408; Swanson et al, 1989 Theor. Appl. Genet. 78:525-530; Newhouse et al (1991) Theor.
- Naturally occurring plant populations that were discovered to be resistant to imidazolinone and/or sulfonylurea herbicides have also been used to develop herbicide-resistant sunflower breeding lines.
- two sunflower lines that are resistant to a sulfonylurea herbicide were developed using germplasm originating from a wild population of common sunflower (Helianthus annuus) as the source of the herbicide-resistance trait (Miller and Al-Khatib (2004) Crop Sci. 44:1037-1038).
- White et al. ((2002) Weed Sci. 50:432-437) had reported that individuals from a wild population of common sunflower from South Dakota, U.S.A.
- AHASL acetohydroxyacid synthase large subunit
- 6,211,439 and 6,222,100 generally describe the use of an altered AHAS gene to elicit herbicide resistance in plants, and specifically discloses certain imidazolinone- resistant corn lines.
- U.S. Patent No. 5,013,659 discloses plants exhibiting herbicide resistance due to mutations in at least one amino acid in one or more conserved regions. The mutations described therein encode either cross-resistance for imidazolinones and sulfonylureas or sulfonylurea-specific resistance, but imidazolinone-specific resistance is not described.
- 5,767,361 discuss an isolated gene having a single amino acid substitution in a wild-type monocot AHAS amino acid sequence that results in imidazolinone-specific resistance.
- rice plants that are resistant to herbicides that interfere with AHAS have been developed by mutation breeding and also by the selection of herbicide-resistant plants from a pool of rice plants produced by anther culture. See, U.S. Patent Nos. 5,545,822, 5,736,629, 5,773,703, 5,773,704, 5,952,553 and 6,274,796.
- the AHAS enzyme is comprised of two subunits: a large subunit (catalytic role) and a small subunit (regulatory role) (Duggleby and Pang (2000) J. Biochem. MoI. Biol. 33:1-36).
- the AHAS large subunit also referred to herein as AHASL
- AHASL may be encoded by a single gene as in the case of Arabidopsis, rice, and sugar beet or by multiple gene family members as in maize, canola, and cotton.
- Specific, single-nucleotide substitutions in the large subunit confer upon the enzyme a degree of insensitivity to one or more classes of herbicides (Chang and Duggleby (1998) Biochem J.
- AHASLl AHASL2
- AHASLS AHASLS
- the herbicide- resistance was due either to the Prol97Leu (using the Arabidopsis AHASL amino acid position nomenclature) substitution or the Ala205 VaI substitution in the AHASLl protein and that each of these substitutions provided resistance to both imidazolinone and sulfonylurea herbicides. Given their high effectiveness and low-to xicity, imidazolinone herbicides are favored for agricultural use.
- imidazolinone herbicides in a particular crop production system depends upon the availability of imidazo lino ne- resistant varieties of the crop plant of interest.
- plant breeders need to develop breeding lines with the imidazo lino ne-resistance trait.
- additional imidazo lino ne-resistant breeding lines and varieties of crop plants, as well as methods and compositions for the production and use of imidazo lino ne-resistant breeding lines and varieties are needed.
- the present invention provides sunflower plants having increased resistance to at least one herbicide when compared to a wild-type sunflower plant.
- the sunflower plants of the invention have increased resistance to at least one imidazolinone herbicide, particularly imazamox and/or imazapyr, when compared to a wild-type sunflower plant.
- the sunflower plants of the present invention comprise a novel herbicide-resistance mechanism that does not involve a mutation in gene encoding an acetohydroxyacid synthase large subunit (AHASL) protein.
- the sunflower plants of the invention also include seeds and progeny plants that comprise at least one copy of a gene or polynucleotide encoding a herbicide-resistant AHASL protein of the invention.
- the present invention provides herbicide-resistant sunflower plants that are from the sunflower line that has been designated herein as MUT31.
- a sample of seeds of the MUT31 line has been deposited with the American Type Culture Collection (ATCC) as ATCC Patent Deposit No. PTA-7839.
- the present invention further provides seeds, progeny plants and other descendent plants that comprise the herbicide-resistance characteristics of MUT31.
- the present invention provides methods for controlling weeds in the vicinity of the herbicide-resistant sunflower plants of the invention.
- the methods comprises applying an effective amount of an herbicide to the weeds and to the herbicide- resistant sunflower plant, wherein the herbicide-resistant sunflower plant has increased resistance to at least one herbicide, particularly an imidazolinone herbicide, more particularly imazamoz, when compared to a wild-type sunflower plant.
- the herbicide can be, for example, applied to the foliage of the plants, to the seeds of the plants prior to planting, and/or to the soil before or after planting.
- the present invention further provides methods for producing a herbicide- resistant sunflower plant.
- the methods involve crossing a first sunflower plant comprising resistance to a herbicide to a second sunflower plant that is not resistant to the herbicide, wherein the first sunflower plant comprises the herbicide-resistance characteristics of MUT31 , particularly a MUT31 sunflower plant or any herbicide- resistant descendent of MUT31.
- a herbicide resistant thereof comprises the herbicide-resistance characteristics of MUT31, representative seeds of MUT 31 having been deposited with the ATCC as Patent Deposit Number PTA-7839.
- the methods further involve selecting for a progeny plant that is resistant to the herbicide.
- the present invention provides methods for increasing the herbicide-resistance of a sunflower plant.
- the methods involve crossing a first sunflower plant comprising resistance to a herbicide to a second sunflower plant, wherein the first sunflower plant comprises the herbicide-resistance characteristics of MUT31 , particularly a MUT31 sunflower plant or any herbicide-resistant descendent of MUT31.
- the second sunflower plant can, but is not required to, comprise resistance to at least one herbicide.
- the methods further involve selecting for a progeny plant that comprises increased herbicide resistance when compared to the herbicide resistance of the second sunflower plant.
- the second sunflower plant comprises at least one herbicide-resistant
- Such a second sunflower plant comprises enhanced resistance one or more AHAS-inhibiting herbicides, particularly an imidazolinone herbicide.
- the sunflower plants were at the 3-4 leaf growth stage at time of malathion and herbicide spraying. Malathion was sprayed on the plants 30 minutes prior to the imazamox spraying. The sunflower plants were evaluated at 7 days after herbicide spraying to determine damage scores. Additional details are provided below in Example 3.
- the present invention relates to sunflower plants having increased resistance to at least one herbicide when compared to wild-type sunflower plants.
- Herbicide- resistant sunflower plants were produced as described hereinbelow by exposing wild- type (with respect to herbicide resistance) sunflower plants to a mutagen, allowing the plants to mature and reproduce, and selecting progeny plants that displayed enhanced resistance to the imidazolinone herbicide, imazamox, when compared to the resistance of a wild-type sunflower plant to the imidazolinone herbicide.
- the invention provides the novel herbicide-resistant sunflower line that is designated herein as MUT31.
- the herbicide-resistant sunflower plants of invention comprise a novel herbicide- resistance mechanism that is independent of a mutation or mutations in one or more AHASL genes.
- the present invention provides a new source of imidazolinone resistance that finds use in methods for controlling weeds and also methods for producing herbicide-resistant sunflower plants or increasing the herbicide resistance of sunflower plants that already comprise herbicide resistance including, but not limited to, imidazolinone-resistance.
- the terms “herbicide-tolerant” and “herbicide- resistant” are used interchangeable and are intended to have an equivalent meaning and an equivalent scope.
- the terms “herbicide-tolerance” and “herbicide- resistance” are used interchangeable and are intended to have an equivalent meaning and an equivalent scope.
- the terms “imidazolinone-resistant” and “imidazolinone-resistance” are used interchangeable and are intended to be of an equivalent meaning and an equivalent scope as the terms “imidazolinone-tolerant” and “imidazolinone-tolerance”, respectively.
- a "herbicide-tolerant” or a “herbicide-resistant” plant is a plant that is tolerant or resistant to at least one herbicide at a level that would normally kill, or inhibit the growth of, a normal or wild-type plant.
- the methods of the present invention for increasing the herbicide resistance of a plant involve the use of sunflower plants comprising herbicide-resistant AHASL polynucleotides and herbicide-resistant
- a “herbicide-resistant AHASL polynucleotide” is a polynucleotide that encodes a herbicide-resistant AHASL protein, wherein the protein comprises herbicide-resistant AHAS activity.
- a “herbicide-resistant AHASL protein” is an AHASL protein that displays higher AHAS activity, relative to the AHAS activity of a wild-type AHASL protein, when in the presence of at least one herbicide that is known to interfere with AHAS activity and at a concentration or level of the herbicide that is to known to inhibit the AHAS activity of the wild-type AHASL protein.
- the AHAS activity of such a herbicide-tolerant or herbicide-resistant AHASL protein may be referred to herein as "herbicide-tolerant" or “herbicide- resistant” AHAS activity.
- a herbicide-tolerant or herbicide-resistant AHASL protein can be introduced into a plant by transforming a plant or ancestor thereof with a nucleotide sequence encoding a herbicide-tolerant or herbicide-resistant AHASL protein.
- Such herbicide-tolerant or herbicide-resistant AHASL proteins are encoded by the herbicide-tolerant or herbicide-resistant AHASL polynucleotides.
- a herbicide-tolerant or herbicide-resistant AHASL protein may occur in a plant as a result of a naturally occurring or induced mutation in an endogenous AHASL gene in the genome of a plant or progenitor thereof.
- Nucleotide sequences encoding herbicide-resistant AHASL proteins and herbicide-resistant plants comprising an endogenous gene that encodes a herbicide-resistant AHASL protein are known in the art. See, for example, U.S. Patent Nos. 5,013,659, 5,731,180, 5,767,361, 5,545,822, 5,736,629, 5,773,703, 5,773,704, 5,952,553 and 6,274,796; all of which are herein incorporated by reference.
- the present invention provides plants, plant tissues, and plant cells with increased resistance or tolerance to at least one herbicide, particularly an imidazolinone herbicide, more particularly imazamox, imazapyr, or both imazamox and imazapyr.
- the preferred amount or concentration of the herbicide is an "effective amount” or “effective concentration.”
- By “effective amount” and “effective concentration” is intended an amount and concentration, respectively, that is sufficient to kill or inhibit the growth of a similar, wild-type, plant, plant tissue, or plant cell, but that said amount does not kill or inhibit as severely the growth of the herbicide- resistant plants, plant tissues, and plant cells of the present invention.
- the effective amount of a herbicide is an amount that is routinely used in agricultural production systems to kill weeds of interest. Such an amount is known to those of ordinary skill in the art, or can be easily determined using methods known in the art.
- the methods of the present invention find use in increasing the herbicide resistance of a sunflower plant, including a sunflower plant that comprises resistance to one or more herbicides, such as, for example, those herbicides that inhibit or otherwise interfere or with the activity of the wild-type AHAS enzyme.
- herbicides may also be referred to herein as "AHAS -inhibiting herbicides” or simply "AHAS inhibitors.”
- AHAS-inhibiting herbicides or an "AHAS inhibitor” is not meant to be limited to single herbicide that interferes with the activity of the AHAS enzyme.
- an "AHAS-inhibiting herbicide” or an “AHAS inhibitor” can be one herbicide or a mixture of two, three, four, or more herbicides, each of which interferes with the activity of the AHAS enzyme.
- wild-type, sunflower plant, plant tissue, or plant cell is intended a sunflower plant, plant tissue, or plant cell, respectively, that lacks the herbicide- resistance characteristics of the herbicide-resistant sunflower plants of the present invention, particularly MUT31 and herbicide-resistant descendents thereof.
- wild-type is not, therefore, intended to imply that a sunflower plant, plant tissue, or plant cell lacks recombinant DNA in its genome, and/or lacks herbicide-resistant characteristics that are different from those disclosed herein.
- plant intended to mean a plant at any developmental stage, as well as any part or parts of a plant that may be attached to or separate from a whole intact plant.
- parts of a plant include, but are not limited to, organs, tissues, and cells of a plant.
- Examples of particular plant parts include a stem, a leaf, a root, an inflorescence, a flower, a floret, a fruit, a pedicle, a peduncle, a stamen, an anther, pollen, a stigma, a style, an ovary, a petal, a sepal, a carpel, a root tip, a root cap, a root hair, a leaf hair, a seed hair, a pollen grain, a microspore, a cotyledon, a hypocotyl, an epicotyl, xylem, phloem, parenchyma, endosperm, a companion cell, a guard cell, and any other known organs, tissues, and cells of a plant. Furthermore, it is recognized that a seed is a plant.
- the sunflower plants of the present invention include both non-transgenic plants and transgenic plants.
- non-transgenic plant is intended to mean a plant lacking recombinant DNA in its genome.
- transgenic plant is intended to mean a plant comprising recombinant DNA in its genome.
- Such a transgenic plant can be produced by introducing recombinant DNA into the genome of the plant.
- progeny of the plant can also comprise the recombinant DNA.
- a progeny plant that comprises at least a portion of the recombinant DNA of at least one progenitor transgenic plant is also a transgenic plant.
- the present invention provides the herbicide-resistant sunflower line that is referred to herein as MUT31.
- the deposit of sunflower line MUT31 was made for a term of at least 30 years and at least 5 years after the most recent request for the furnishing of a sample of the deposit is received by the ATCC.
- the deposit will be maintained under the terms of the Budapest Treaty on the International Recognition of the Deposit of Microorganisms for the Purposes of Patent Procedure.
- sunflower line MUT31 was made for a term of at least 30 years and at least 5 years after the most recent request for the furnishing of a sample of the deposit is received by the ATCC. Additionally, Applicants have satisfied all the requirements of 37 CF. R. ⁇ 1.801-1.809, including providing an indication of the viability of the sample.
- the present invention provides herbicide-resistant sunflower plants of the MUT31 line that were produced by a mutation breeding. Wild-type sunflower plants were mutagenized by exposing the plants to a mutagen, particularly a chemical mutagen, more particularly ethyl methanesulfonate (EMS).
- EMS ethyl methanesulfonate
- the present invention is not limited to herbicide-resistant sunflower plants that are produced by a mutagenesis method involving the chemical mutagen EMS. Any mutagenesis method known in the art may be used to produce the herbicide-resistant sunflower plants of the present invention.
- Such mutagenesis methods can involve, for example, the use of any one or more of the following mutagens: radiation, such as X-rays, Gamma rays (e.g., cobalt 60 or cesium 137), neutrons, (e.g., product of nuclear fission by uranium 235 in an atomic reactor), Beta radiation (e.g., emitted from radioisotopes such as phosphorus 32 or carbon 14), and ultraviolet radiation (preferably from 2500 to 2900 nm), and chemical mutagens such as base analogues (e.g., 5 -bromo -uracil), related compounds (e.g., 8-ethoxy caffeine), antibiotics (e.g., streptonigrin), alkylating agents (e.g., sulfur mustards, nitrogen mustards, epoxides, ethylenamines, sulfates, sulfonates, sulfones, lactones), azide, hydroxy
- Herbicide-resistant plants can also be produced by using tissue culture methods to select for plant cells comprising herbicide-resistance mutations and then regenerating herbicide-resistant plants therefrom. See, for example, U.S. Patent Nos. 5,773,702 and 5,859,348, both of which are herein incorporated in their entirety by reference. Further details of mutation breeding can be found in "Principals of Cultivar Development” Fehr, 1993 Macmillan Publishing Company the disclosure of which is incorporated herein by reference.
- Sunflower plants and seeds of the present invention comprise the herbicide resistance characteristics of MUT31. Such plants and seeds may be referred to herein as comprising the MUT31 trait.
- the MUT31 trait confers resistance to imidazolinone herbicides to a plant or seed possessing this trait whether the trait is in the heterozygous or homozygous state in the plant.
- the MUT31 trait confers to a sunflower plant or seed resistance to at least one imidazolinone herbicide, particularly imazamox and/or imazapyr, wherein the imidazolinone resistance is reduced or inhibited by the organophosphate insecticide malathion.
- sunflower plants and seeds comprising the MUT31 trait comprise malathion-inhibitable, imidazolinone resistance. While the present invention is not bound to a particular biological mechanism, the herbicide resistance of MUT31 sunflower plants or the MUT31 trait is believed to be due to an induced mutation in a single gene within the sunflower nuclear genome.
- the sunflower plants of the present invention include, for example, descendents of the MUT31 line that are heterozygous or homozymgous for the MUT31 trait. It is recognized that such descendents can be produced via sexual reproduction or by any asexual reproduction methods known in the art such as for example, tissue culture. Descendents of the MUT31 line that comprise the MUT31 trait can be identified by determining if a descendent sunflower plant comprises malathion-inhibitable, imidazolinone resistance. The present invention does not depend on a particular method for determining if a descendent sunflower plant comprises malathion-inhibitable, imidazolinone resistance.
- any method know in the art can be used including the method disclosed in Example 3 below to determine if a descendent of MUT31 comprises malathion-inhibitable, imidazolinone resistance.
- the method involves applying malathion to a descendent of MUT31 prior to the application of the imidazolinone herbicide and determining whether malathion reduces or inhibits the resistance of the descendent to the imidazolinone herbicide.
- Descendents that comprise the MUT31 trait comprise malathion-inhibitable, imidazolinone resistance.
- the sunflower plants of the present invention include, for example, those sunflower plants that are descendents of MUT31 and comprise malathion-inhibitable, imidazolinone resistance.
- the present invention provides methods for enhancing the tolerance or resistance of a sunflower plant, plant tissue, plant cell, or other host cell to at least one herbicide, particularly an imidazolinone herbicide or mixture two or more imidazolinone herbicides.
- the imidazolinone herbicides include, but are not limited to, PURSUIT® (imazethapyr), CADRE® (imazapic), RAPTOR® (imazamox), SCEPTER® (imazaquin), ASSERT® (imazethabenz), ARSENAL® (imazapyr), a derivative of any of the aforementioned herbicides, and a mixture of two or more of the aforementioned herbicides, for example, imazapyr/imazamox (ODYSSEY®).
- the imidazolinone herbicide can be selected from, but is not limited to, 2- (4-isopropyl-4-methyl-5-oxo-2- imidiazolin-2-yl) -nicotinic acid, [2- (4-isopropyl)-4-] [methyl-5-oxo-2-imidazolin-2- yl)-3-quinolinecarboxylic] acid, [5-ethyl-2- (4-isopropyl-] 4-methyl-5-oxo-2- imidazolin-2-yl) -nicotinic acid, 2- (4-isopropyl-4-methyl-5-oxo-2- imidazolin-2-yl)- 5- (methoxymethyl)-nicotinic acid, [2- (4-isopropyl-4-methyl-5-oxo-2-] imidazolin-2- yl)-5-methylnicotinic acid, and a mixture of methyl [6- (4-isopropyl-4-]
- the present invention further provides a method for controlling weeds in the vicinity of a herbicide-resistant sunflower plant of the invention.
- the method comprises applying an effective amount of a herbicide to the weeds and to the herbicide-resistant sunflower plant, wherein the plant has increased resistance to at least one herbicide, particularly an imidazolinone or sulfonylurea herbicide, when compared to a wild-type plant.
- the herbicide-resistant plants of the invention are preferably crop plants, including, but not limited to, sunflower, alfalfa, Brassica sp., soybean, cotton, safflower, peanut, tobacco, tomato, potato, wheat, rice, maize, sorghum, barley, rye, millet, and sorghum.
- herbicides particularly imidazolinone and sulfonylurea herbicides
- a herbicide can be used by itself for pre-emergence, post- emergence, pre-planting and at planting control of weeds in areas surrounding the plants described herein or an imidazolinone herbicide formulation can be used that contains other additives.
- the herbicide can also be used as a seed treatment. That is an effective concentration or an effective amount of the herbicide, or a composition comprising an effective concentration or an effective amount of the herbicide can be applied directly to the seeds prior to or during the sowing of the seeds.
- Additives found in an imidazolinone or sulfonylurea herbicide formulation or composition include other herbicides, detergents, adjuvants, spreading agents, sticking agents, stabilizing agents, or the like.
- the herbicide formulation can be a wet or dry preparation and can include, but is not limited to, flowable powders, emulsif ⁇ able concentrates and liquid concentrates.
- the herbicide and herbicide formulations can be applied in accordance with conventional methods, for example, by spraying, irrigation, dusting, coating, and the like.
- the present invention provides seeds with increased resistance to at least one herbicide, particularly an imidazolinone herbicide. Such seeds include, for example, sunflower seeds that are herbicide-resistant descendents of the MUT31 sunflower line.
- a "descendent" of the MUT31 sunflower line comprises any plant, plant cell, or plant part that is derived by sexual and/or asexual propagation from MUT31 , representative seeds of MUT31 having been deposited with the ATCC and assigned ATCC Patent Deposit No. PTA-7839.
- a descendent includes a plant produced by crossing a first plant with a second plant to produce a seed of the third plant (i.e., a descendent), wherein the first plant is a MUT31 sunflower plant and the second is another sunflower plant that is not a MUT31 sunflower plant.
- Such a descendent also includes any plants that are descended from the third plant whether produced by sexual and/or asexual propagation.
- cells, tissue or an organ from the third plant could be used to produce a fourth plant by an asexual propagation method including, but not limited to, in vitro plant cell, tissue, and organ culture methods and methods involving the rooting of stem cuttings.
- a herbicide-resistant descendent of MUT31 is a descendent of MUT31 that comprises the herbicide-resistance characteristics of MUT31 by way of being descended from one or more MUT31 sunflower plants.
- a herbicide-resistant descendent has inherited the herbicide- resistance characteristics of a MUT31 sunflower plant by sexual reproduction, asexual reproduction, or combination thereof.
- the "progeny" of a plant includes a plant of any subsequent generation whose ancestry can be traced to that plant.
- the "herbicide resistant progeny” of MUT31 includes a plant of any subsequent generation whose ancestry can be traced to MUT31 and that comprises the herbicide-resistance characteristics of MUT31 by way of that ancestry.
- the present invention provides methods for producing a herbicide-resistant sunflower plant, particularly a herbicide-resistant sunflower plant, through conventional plant breeding involving sexual reproduction. The methods comprise crossing a first plant comprises resistance to a herbicide to a second plant that is not resistant to the herbicide.
- the first plant can be any of the herbicide-resistant plants of the present invention including, for example, sunflower plants that comprise the herbicide-resistance characteristics of the MUT31 sunflower plant, particularly MUT31 sunflower plants and herbicide-resistant descendents of MUT31.
- the second plant can be any plant that is capable of producing viable progeny plants (i.e., seeds) when crossed with the first plant. Typically, but not necessarily, the first and second plants are of the same species.
- the methods of the invention can further involve one or more generations of backcrossing the progeny plants of the first cross to a plant of the same line or genotype as either the first or second plant.
- the progeny of the first cross or any subsequent cross can be crossed to a third plant that is of a different line or genotype than either the first or second plant.
- the methods of the invention can additionally involve selecting plants that comprise the herbicide- resistance characteristics of the first plant.
- the present invention further provides methods for increasing the herbicide- resistance of a plant, particularly a herbicide-resistant sunflower plant, through conventional plant breeding involving sexual reproduction.
- the methods comprise crossing a first plant comprises resistance to a herbicide to a second plant that may or may not be resistant to the herbicide or may be resistant to a different herbicide or herbicides than the first plant.
- the first plant can be any of the herbicide-resistant sunflower plants of the present invention including, for example, a MUT31 sunflower plants and a herbicide-resistant descendent thereof.
- the second plant can be any plant that is capable of producing viable progeny plants (i.e., seeds) when crossed with the first plant. Typically, but not necessarily, the first and second plants are of the same species.
- the progeny plants produced by this method of the present invention have increased or enhanced resistance to a herbicide when compared to either the first or second plant or both.
- the progeny plants will have the combined herbicide-resistance characteristics of the first and second plants.
- the methods of the invention can further involve one or more generations of backcrossing the progeny plants of the first cross to a plant of the same line or genotype as either the first or second plant.
- the progeny of the first cross or any subsequent cross can be crossed to a third plant that is of a different line or genotype than either the first or second plant.
- the methods of the invention can additionally involve selecting plants that comprise the herbicide-resistance characteristics of the first plant, the second plant, or both the first and the second plant.
- the present invention provides methods for enhancing or increasing the resistance of a sunflower plant to at least one imidazolinone herbicide.
- Imidazolinone herbicides are known as AHAS-inhibiting herbicides because of their recognized ability to inhibit AHAS activity in vivo and in vitro.
- AHAS-inhibiting herbicides include, for example, sulfonylurea herbicides, triazolopyrimidine herbicides, pyrimidinyloxybenzoate herbicides, and sulfonylamino-carbonyltriazolinone herbicides.
- the methods involve enhancing or increasing the resistance of a herbicide-resistant sunflower plant that comprises resistance to an AHAS-inhibiting herbicide, wherein the resistance to the AHAS- inhibiting herbicide is due to one or more herbicide-resistant AHASL proteins.
- a herbicide-resistant sunflower plant can be resistant to one or more AHAS-inhibiting herbicides such as, for example, an imidazolinone herbicide, a sulfonylurea herbicide, a triazolopyrimidine herbicide, a pyrimidinyloxybenzoate herbicide, a sulfonylamino- carbonyltriazolinone herbicide, or mixture thereof.
- Sulfonylurea herbicides include, but are not limited to, chlorsulfuron, metsulfuron methyl, sulfometuron methyl, chlorimuron ethyl, thifensulfuron methyl, tribenuron methyl, bensulfuron methyl, nicosulfuron, ethametsulfuron methyl, rimsulfuron, triflusulfuron methyl, triasulfuron, primisulfuron methyl, cinosulfuron, amidosulf ⁇ uon, fluzasulfuron, imazosulfuron, pyrazosulfuron ethyl, halosulfuron, azimsulfuron, cyclosulfuron, ethoxysulfuron, flazasulfuron, flupyrsulfuron methyl, foramsulfuron, iodosulfuron, o
- the triazolopyrimidine herbicides include, but are not limited to, cloransulam, diclosulam, florasulam, flumetsulam, metosulam, and penoxsulam.
- the pyrimidinyloxybenzoate herbicides of the invention include, but are not limited to, bispyribac, pyrithiobac, pyriminobac, pyribenzoxim and pyriftalid.
- the sulfonylamino-carbonyltriazolinone herbicides include, but are not limited to, flucarbazone and propoxycarbazone.
- pyrimidinyloxybenzoate herbicides are closely related to the pyrimidinylthiobenzoate herbicides and are generalized under the heading of the latter name by the Weed Science Society of America. Accordingly, the herbicides of the present invention further include pyrimidinylthiobenzoate herbicides, including, but not limited to, the pyrimidinyloxybenzoate herbicides described above.
- the sunflower plants of the present invention can be non-transgenic or transgenic.
- non-transgenic sunflower plants having increased resistance to at least one imidazolinone herbicide include the MUT31 sunflower plant, representative seeds of MUT31 having been deposited with the ATCC as Patent Deposit No. PTA-7839; or mutant, recombinant, or a genetically engineered derivative of MUT31; or of any progeny of MUT31; or a plant that is a progeny of any of these plants; or a plant that comprises the herbicide-resistance characteristics of MUT31 , particularly a herbicide-resistant descendent of MUT31.
- transgenic sunflower plant having increased resistance to at least one imidazolinone herbicide is a sunflower plant that is a genetically engineered derivative of MUT31 that comprises the herbicide-resistance characteristics of MUT31.
- a genetically engineered derivative can comprises in its genome, for example, a transgene of interest including, but not limited to, a herbicide-resistant AHASL gene, a gene conferreing disease resistance, and a gene conferreing insect resistance.
- the present invention provides methods that involve the use of an imidazolinone herbicide.
- the imidazolinone herbicide can be applied by any method known in the art including, but not limited to, seed treatment, soil treatment, and foliar treatment.
- the imidazolinone herbicide can be converted into the customary formulations, for example solutions, emulsions, suspensions, dusts, powders, pastes and granules.
- the use form depends on the particular intended purpose; in each case, it should ensure a fine and even distribution of the compound according to the invention.
- the formulations are prepared in a known manner (see e.g. for review US 3,060,084, EP-A 707 445 (for liquid concentrates), Browning, "Agglomeration”, Chemical Engineering, Dec. 4, 1967, 147-48, Perry's Chemical Engineer's Handbook, 4th Ed., McGraw-Hill, New York, 1963, pages 8-57 and et seq.
- auxiliaries suitable for the formulation of agrochemicals such as solvents and/or carriers, if desired emulsif ⁇ ers, surfactants and dispersants, preservatives, antifoaming agents, anti-freezing agents, for seed treatment formulation also optionally colorants and/or binders and/or gelling agents.
- solvents examples include water, aromatic solvents (for example Solvesso products, xylene), paraffins (for example mineral oil fractions), alcohols (for example methanol, butanol, pentanol, benzyl alcohol), ketones (for example cyclohexanone, gamma-butyro lactone), pyrrolidones (NMP, NOP), acetates (glycol diacetate), glycols, fatty acid dimethylamides, fatty acids and fatty acid esters.
- solvent mixtures may also be used.
- suitable carriers are ground natural minerals (for example kaolins, clays, talc, chalk) and ground synthetic minerals (for example highly disperse silica, silicates).
- Suitable emulsif ⁇ ers are nonionic and anionic emulsif ⁇ ers (for example polyoxyethylene fatty alcohol ethers, alkylsulfonates and arylsulfo nates).
- examples of dispersants are lignin- sulfite waste liquors and methylcellulose.
- Suitable surfactants used are alkali metal, alkaline earth metal and ammonium salts of lignosulfonic acid, naphthalenesulfonic acid, phenolsulfonic acid, dibutylnaphthalenesulfonic acid, alkylarylsulfonates, alkyl sulfates, alkylsulfonates, fatty alcohol sulfates, fatty acids and sulfated fatty alcohol glycol ethers, furthermore condensates of sulfonated naphthalene and naphthalene derivatives with formaldehyde, condensates of naphthalene or of naphthalenesulfonic acid with phenol and formaldehyde, polyoxyethylene octylphenol ether, ethoxylated isooctylphenol, octylphenol, nonylphenol, alkylphenol polyglycol ethers, tributylphenyl polyg
- Substances which are suitable for the preparation of directly sprayable solutions, emulsions, pastes or oil dispersions are mineral oil fractions of medium to high boiling point, such as kerosene or diesel oil, furthermore coal tar oils and oils of vegetable or animal origin, aliphatic, cyclic and aromatic hydrocarbons, for example toluene, xylene, paraffin, tetrahydronaphthalene, alkylated naphthalenes or their derivatives, methanol, ethanol, propanol, butanol, cyclohexanol, cyclohexanone, isophorone, highly polar solvents, for example dimethyl sulfoxide, N- methylpyrrolidone or water.
- mineral oil fractions of medium to high boiling point such as kerosene or diesel oil, furthermore coal tar oils and oils of vegetable or animal origin, aliphatic, cyclic and aromatic hydrocarbons, for example toluene, xylene, par
- anti- freezing agents such as glycerin, ethylene glycol, propylene glycol and bactericides such as can be added to the formulation.
- Suitable antifoaming agents are for example antifoaming agents based on silicon or magnesium stearate.
- Suitable preservatives are for example Dichlorophen und enzy lalkoho lhemiformal .
- Seed Treatment formulations may additionally comprise binders and optionally colorants.
- Binders can be added to improve the adhesion of the active materials on the seeds after treatment.
- Suitable binders are block copolymers EO/PO surfactants but also polyvinylalcoholsl, polyvinylpyrrolidones, polyacrylates, polymethacrylates, polybutenes, polyisobutylenes, polystyrene, polyethyleneamines, polyethyleneamides, polyethyleneimines (Lupasol®, Polymin®), polyethers, polyurethans, polyvinylacetate, tylose and copolymers derived from these polymers.
- colorants can be included in the formulation.
- Suitable colorants or dyes for seed treatment formulations are Rhodamin B, C.I. Pigment Red 112, C.I. Solvent Red 1, pigment blue 15:4, pigment blue 15:3, pigment blue 15:2, pigment blue 15:1, pigment blue 80, pigment yellow 1, pigment yellow 13, pigment red 112, pigment red 48:2, pigment red 48:1, pigment red 57:1, pigment red 53:1, pigment orange 43, pigment orange 34, pigment orange 5, pigment green 36, pigment green 7, pigment white 6, pigment brown 25, basic violet 10, basic violet 49, acid red 51, acid red 52, acid red 14, acid blue 9, acid yellow 23, basic red 10, basic red 108.
- a suitable gelling agent is carrageen (Satiagel ® )
- Powders, materials for spreading, and dustable products can be prepared by mixing or concomitantly grinding the active substances with a solid carrier.
- Granules for example coated granules, impregnated granules and homogeneous granules, can be prepared by binding the active compounds to solid carriers.
- solid carriers are mineral earths such as silica gels, silicates, talc, kaolin, attaclay, limestone, lime, chalk, bole, loess, clay, dolomite, diatomaceous earth, calcium sulfate, magnesium sulfate, magnesium oxide, ground synthetic materials, fertilizers, such as, for example, ammonium sulfate, ammonium phosphate, ammonium nitrate, ureas, and products of vegetable origin, such as cereal meal, tree bark meal, wood meal and nutshell meal, cellulose powders and other solid carriers.
- mineral earths such as silica gels, silicates, talc, kaolin, attaclay, limestone, lime, chalk, bole, loess, clay, dolomite, diatomaceous earth
- the formulations comprise from 0.01 to 95% by weight, preferably from 0.1 to 90% by weight, of the imidazolinone herbicide.
- the imidazolinone herbicides are employed in a purity of from 90% to 100% by weight, preferably 95% to 100% by weight (according to NMR spectrum).
- respective formulations can be diluted 2-10 fold leading to concentrations in the ready to use preparations of 0.01 to 60% by weight active compound by weight, preferably 0.1 to 40% by weight.
- the imidazolinone herbicide can be used as such, in the form of their formulations or the use forms prepared therefrom, for example in the form of directly sprayable solutions, powders, suspensions or dispersions, emulsions, oil dispersions, pastes, dustable products, materials for spreading, or granules, by means of spraying, atomizing, dusting, spreading or pouring.
- the use forms depend entirely on the intended purposes; they are intended to ensure in each case the finest possible distribution of the imidazolinone herbicide according to the invention.
- Aqueous use forms can be prepared from emulsion concentrates, pastes or wettable powders (sprayable powders, oil dispersions) by adding water.
- emulsions, pastes or oil dispersions the substances, as such or dissolved in an oil or solvent, can be homogenized in water by means of a wetter, tackifier, dispersant or emulsifier.
- concentrates composed of active substance, wetter, tackifier, dispersant or emulsif ⁇ er and, if appropriate, solvent or oil and such concentrates are suitable for dilution with water.
- the active compound concentrations in the ready-to-use preparations can be varied within relatively wide ranges. In general, they are from 0.0001 to 10%, preferably from 0.01 to 1% per weight.
- the imidazolinone herbicide may also be used successfully in the ultra- low- volume process (ULV), it being possible to apply formulations comprising over 95% by weight of active compound, or even to apply the active compound without additives.
- UUV ultra- low- volume process
- Products for dilution with water for foliar applications may be applied to the seed diluted or undiluted.
- a dispersant for example polyvinylpyrrolidone. Dilution with water gives a dispersion, whereby a formulation with 20% (w/w) of imidazolinone herbicide is obtained.
- Emulsions EW, EO, ES
- Fifty parts by weight of the imidazolinone herbicide are ground finely with addition of 50 parts by weight of dispersants and wetters and made as water-dispersible or water-soluble 25 granules by means of technical appliances (for example extrusion, spray tower, fluidized bed). Dilution with water gives a stable dispersion or solution of the imidazolinone herbicide, whereby a formulation with 50% (w/w) of imidazolinone herbicide is obtained.
- A) Dustable powders (DP, DS) Five parts by weight of the imidazolinone herbicide are ground finely and mixed intimately with 95 parts by weight of finely divided kaolin. This gives a dustable product having 5% (w/w) of imidazolinone herbicide.
- One-half part by weight of the imidazolinone herbicide is ground finely and associated with 95.5 parts by weight of carriers, whereby a formulation with 0.5% (w/w) of imidazolinone herbicide is obtained.
- Current methods are extrusion, spray-drying or the fluidized bed. This gives granules to be applied undiluted for foliar use.
- Conventional seed treatment formulations include for example flowable concentrates FS, solutions LS, powders for dry treatment DS, water dispersible powders for slurry treatment WS, water-soluble powders SS and emulsion ES and EC and gel formulation GF. These formulations can be applied to the seed diluted or undiluted. Application to the seeds is carried out before sowing, either directly on the seeds.
- a FS formulation is used for seed treatment.
- a FS formulation may comprise 1-800 g/1 of active ingredient, 1-200 g/1 Surfactant, 0 to 200 g/1 antifreezing agent, 0 to 400 g/1 of binder, 0 to 200 g/1 of a pigment and up to 1 liter of a solvent, preferably water.
- the present invention provides seeds of the herbicide-resistant plants of the present invention, particularly seeds that are herbicide-resistant descendents of MUT31.
- seeds of the present invention are treated with herbicides, preferably herbicides selected from the group consisting of AHAS- inhibiting herbicides such as amidosulfuron, azimsulfuron, bensulfuron, chlorimuron, chlorsulfuron, cinosulfuron, cyclosulfamuron, ethametsulfuron, ethoxysulfuron, flazasulfuron, flupyrsulfuron, foramsulfuron, halosulfuron, imazosulfuron, iodosulfuron, mesosulfuron, metsulfuron, nicosulfuron, oxasulfuron, primisulfuron, prosulfuron, pyrazosulfuron, rimsulfuron, sulfometuron, sulfosul
- seed treatment comprises all suitable seed treatment techniques known in the art, such as seed dressing, seed coating, seed dusting, seed soaking, and seed pelleting.
- a further subject of the invention is a method of treating soil by the application, in particular into the seed drill: either of a granular formulation containing the imidazolinone herbicide as a composition/formulation (e.g. a granular formulation, with optionally one or more solid or liquid, agriculturally acceptable carriers and/or optionally with one or more agriculturally acceptable surfactants.
- This method is advantageously employed, for example, in seedbeds of cereals, maize, cotton, and sunflower.
- the present invention also comprises seeds coated with or containing with a seed treatment formulation comprising at least one ALS inhibitor selected from the group consisting of amidosulfuron, azimsulfuron, bensulfuron, chlorimuron, chlorsulfuron, cinosulfuron, cyclosulfamuron, ethametsulfuron, ethoxysulfuron, flazasulfuron, flupyrsulfuron, foramsulfuron, halosulfuron, imazosulfuron, iodosulfuron, mesosulfuron, metsulfuron, nicosulfuron, oxasulfuron, primisulfuron, prosulfuron, pyrazosulfuron, rimsulfuron, sulfometuron, sulfosulfuron, thifensulfuron, triasulfuron, tribenuron, trifloxysulfuron, triflusulfuron
- seed embraces seeds and plant propagules of all kinds including but not limited to true seeds, seed pieces, suckers, corms, bulbs, fruit, tubers, grains, cuttings, cut shoots and the like and means in a preferred embodiment true seeds.
- coated with and/or containing generally signifies that the active ingredient is for the most part on the surface of the propagation product at the time of application, although a greater or lesser part of the ingredient may penetrate into the propagation product, depending on the method of application. When the said propagation product is (re)planted, it may absorb the active ingredient.
- the seed treatment application with the imidazolinone herbicide or with a formulation comprising the imidazolinone herbicide is carried out by spraying or dusting the seeds before sowing of the plants and before emergence of the plants.
- the corresponding formulations are applied by treating the seeds with an effective amount of the imidazolinone herbicide or a formulation comprising the imidazolinone herbicide.
- the application rates are generally from 0.1 g to 10 kg of the a.i. (or of the mixture of a.i. or of the formulation) per 100 kg of seed, preferably from 1 g to 5 kg per 100 kg of seed, in particular from 1 g to 2.5 kg per 100 kg of seed.
- the present invention provides a method for combating undesired vegetation or controlling weeds comprising contacting the seeds of the resistant plants according to the present invention before sowing and/or after pregermination with an imidazolinone herbicide.
- the method can further comprise sowing the seeds, for example, in soil in a field or in a potting medium in greenhouse. The method finds particular use in combating undesired vegetation or controlling weeds in the immediate vicinity of the seed.
- control of undesired vegetation is understood as meaning the killing of weeds and/or otherwise retarding or inhibiting the normal growth of the weeds.
- Weeds in the broadest sense, are understood as meaning all those plants which grow in locations where they are undesired.
- the weeds of the present invention include, for example, dicotyledonous and monocotyledonous weeds.
- Dicotyledonous weeds include, but are not limited to, weeds of the genera: Sinapis, Lepidium, Galium, Stellaria, Matricaria, Anthemis, Galinsoga, Chenopodium, Urtica, Senecio, Amaranthus, Portulaca, Xanthium,
- Monocotyledonous weeds include, but are not limited to, weeds of the genera: Echinochloa, Setaria, Panicum, Digitaria, Phleum, Poa, Festuca, Eleusine, Brachiaria, Lolium, Bromus, Avena, Cyperus, Sorghum, Agropyron, Cynodon, Monochoria, Fimbristyslis, Sagittaria, Eleocharis, Scirpus, Paspalum, Ischaemum, Sphenoclea, Dactyloctenium, Agrostis, Alopecurus, and Apera.
- the weeds of the present invention can include, for example, crop plants that are growing in an undesired location.
- a volunteer maize plant that is in a field that predominantly comprises soybean plants can be considered a weed, if the maize plant is undesired in the field of soybean plants.
- EXAMPLE 1 Mutagenesis of Helianthus annuus Line RHA266 and Selection of
- EMS ethyl methanesulfonate
- EMS is a known mutagen that typically induces G-C-to-A-T transitions in DNA (Jander et al. (2003) Plant Physiol. 131 : 139-146). Plants were treated with a solution comprising 0.5%, 5%, or 10%, (w/v) EMS.
- Tissue samples were taken from each individual surviving Mi plant and DNA from each sample was extracted for PCR amplification and sequencing studies described below in Example 2.
- M 2 seeds that were produced by each of the forty- four fertile Mi plants were sown in individual plots in Fargo, ND then sprayed with 0.5 X of SWEEPER 70DG (25 g ai/ha imazamox) at the 2-4 leaf pair growth stage.
- One of the plots was selected as homozygous tolerant and designated as MUT31.
- M 2 plants of MUT31 were harvested, their M 2: 3 progenies sown in Balcarce in the summer of 2003-2004, and the resulting plants allowed to mature and then selfed.
- M 4 seed from one plot was harvested and declared breeder seed on the basis of phenotypic observations. (Breeder seed is seed produced by the direct control of the plant breeder and is the basis of the first and recurring increase of foundation seeds.)
- PCR polymerase chain reaction
- a field trial was conducted to compare the herbicide tolerance of sunflower hybrids carrying the MUT31 trait and the A205 V mutation in a sunflower AHASL gene (A205V/ A205V).
- a sunflower AHASL gene with the A205V mutation encodes a AHASL protein which has a valine at amino acid that corresponds to amino acid position 205 in the Arabidopsis thaliana AHASL protein.
- the amino acid at the same position in a wild-type sunflower AHASL protein is alanine. In the amino acid sequence of the sunflower AHASL protein, this alanine-to-valine amino acid substitution is at amino acid position 190.
- Seed from each entry was produced under optimum seed production conditions in South America in 2005/2006.
- the field trial was conducted at one location in North Dakota, USA in 2006.
- the entries were organized in a randomized complete block using a split plot design consisting of 3 replications for each treatment combination.
- Factor A was the herbicide treatment
- factor B was the sunflower entry.
- the plot size was 4 rows x 12 ft and the seeding rate was consistent with local agronomic practices.
- the herbicide rates for each treatment for Entries 1-6 are shown in Table 6.
- the herbicide rates for each treatment for Entries 8 are shown in Table 7.
- the spray volume was 10 gallons per acre (GPA) (or 100 liters/ha) for a backpack sprayer or 20 GPA (or 200 liters/ha) for a tractor mounted boom.
- the herbicide treatments were applied at the 2-4 leaf growth stage.
- NIS non-ionic surfactant
- Phytotoxicity ratings were assessed at 7 days and 21 days following herbicide application. Phytotoxicity was recorded as the amount of plant damage (in percent), where a rating of '0' indicated no damage to the plants in the plot relative to the untreated plot. A rating of '100' indicated complete necrosis (death) of the plants in the plot relative to the untreated plot.
- the phytotoxicity in the heterozygous A205V entries was significantly higher than the double heterozygous A205 V/MUT31 entry (entry 6) at 21 days after treatment with imazamox and imazapyr.
- the homozygous A205V entries (entries 1-2) demonstrated the lowest levels of phytotoxicity or crop injury (Table 1). At 100 g ai/ha of imazamox the range in phytotoxicity of the A205V heterozygous entries was between 25% and 47% compared to an injury rating of 10% for the A205V / MUT31 heterozygous entry.
- the range in phytotoxicity of the A205V heterozygous entries was between 73% and 78% injury compared to an injury rating of 43% for the A205V / MUT31 heterozygous entry.
- the range in phytotoxicity of the A205V heterozygous entries was between 20% and 43% injury compared to an injury rating of 10% for the A205V / MUT31 heterozygous entry.
- MUT31 When MUT31 alone (entry 8) was challenged with 37.5 g ai/ha of imazamox, it demonstrated an injury rating of 47% at 21 days after treatment. From previous studies (data not shown), MUT31 has demonstrated 100% crop injury at rates of 75 g ai of imazamox per hectare and 100 g ai of imazapyr per hectare.
- the double heterozygous A205 V/MUT31 entries demonstrated significantly higher herbicide tolerance to both imazamox and imazapyr treatments versus the heterozygous A205 V/- entries and versus the MUT31 entry on its own.
- MUT31 when stacked with the A205 V mutation in the heterozygous state provides stronger (enhanced) herbicide tolerance than the A205V mutation in the heterozygous state.
- Having a product that works in the heterozygous state at 2x the commercial product rate (100 g ai imazamox/ha and 160 g ai imazapyr/ha) is a great advantage to sunflower hybrid plant breeders over the current homozygous A205V/A205V product, saving both time and resources in the breeding of imadazolinone tolerant sunflowers.
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Priority Applications (12)
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UAA200906898A UA108733C2 (en) | 2006-12-12 | 2007-11-12 | Sunflower herbicide tolerant to herbicide |
ES07848061.3T ES2581987T3 (en) | 2006-12-12 | 2007-12-11 | Herbicide resistant sunflower plants and methods of use |
AU2007331535A AU2007331535A1 (en) | 2006-12-12 | 2007-12-11 | Herbicide-resistant sunflower plants and methods of use |
EP07848061.3A EP2091319B1 (en) | 2006-12-12 | 2007-12-11 | Herbicide-resistant sunflower plants and methods of use |
BRPI0720338-1A2A BRPI0720338A2 (en) | 2006-12-12 | 2007-12-11 | HERBICIDE RESISTANT SUNFLOWER PLANT, SUNFLOWER PLANT SEED, PLANT CELL, PLANT POLLEN GRAIN, METHODS FOR CONTROLING WEED HERBS IN THE NEIGHBORHOOD TO PRODUCE A SUNFLOWER PLANT HERBICIDE OF A SUNFLOWER PLANT, TO COMBAT UNWANTED VEGETATION AND TO PRODUCE A HYBRID SUNFLOWER SEED, DESIGNED SUNFLOWER LINE, SUNFLOWER PLANT OR PART OF THE SAME, TISSUE CROP, AND PROTOPLAST. |
JP2009540756A JP2010512157A (en) | 2006-12-12 | 2007-12-11 | Herbicide resistant sunflower plants and methods of use |
EA200900768A EA019478B1 (en) | 2006-12-12 | 2007-12-11 | Herbicide-resistant sunflower plants and methods of use |
CA002672227A CA2672227A1 (en) | 2006-12-12 | 2007-12-11 | Herbicide-resistant sunflower plants and methods of use |
CN200780049043.7A CN101657090B (en) | 2006-12-12 | 2007-12-11 | Herbicide-resistant sunflower plants and methods of use |
US12/517,919 US9035133B2 (en) | 2006-12-12 | 2007-12-11 | Herbicide-resistant sunflower plants and methods of use |
ZA2009/04820A ZA200904820B (en) | 2006-12-12 | 2009-07-09 | Herbicide-resistant sunflower plants and methods of use |
US14/606,219 US20150211017A1 (en) | 2006-12-12 | 2015-01-27 | Herbicide-resistant sunflower plants and methods of use |
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CL (1) | CL2007003605A1 (en) |
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2009046334A1 (en) | 2007-10-05 | 2009-04-09 | Cibus Llc | Mutated acetohydroxyacid synthase genes in brassica |
WO2013004280A1 (en) | 2011-07-01 | 2013-01-10 | Advanta International Bv | Isolated mutated nucleotide sequences that encode a modified oleate destaurase sunflower protein, modified protein, methods and uses |
WO2013004281A1 (en) | 2011-07-01 | 2013-01-10 | Advanta International Bv | Nucleotide sequences mutated by insertion that encode a truncated oleate desaturase protein, proteins, methods and uses |
JP2013514791A (en) * | 2009-12-22 | 2013-05-02 | バイエル・クロップサイエンス・エヌ・ヴェー | Herbicide-tolerant plants |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ES2692594T1 (en) * | 2005-03-02 | 2018-12-04 | Instituto Nacional De Tecnologia Agropecuaria | Rice plants resistant to herbicides, polynucleotides that encode herbicide-resistant proteins of the large subunit of acetohydroxy acid synthase and methods for their use |
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CN107484591A (en) * | 2017-08-30 | 2017-12-19 | 吉林省农业科学院 | The method for integrated control of sunflower weeds in field |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1999019493A2 (en) * | 1997-10-10 | 1999-04-22 | North Carolina State University | Cytochrome p-450 constructs and methods of producing herbicide-resistant transgenic plants |
WO2001065922A2 (en) * | 2000-03-09 | 2001-09-13 | E. I. Du Pont De Nemours And Company | Sulfonylurea-tolerant sunflower plants |
WO2006024351A1 (en) * | 2004-07-30 | 2006-03-09 | Basf Agrochemical Products B.V. | Herbicide-resistant sunflower plants, plynucleotides encoding herbicide-resistant acetohydroxy acid synthase large subunit proteins, and methods of use |
Family Cites Families (107)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4443971A (en) | 1979-10-16 | 1984-04-24 | Cornell Research Foundation, Inc. | Herbicide-tolerant plants |
HU187284B (en) | 1980-12-11 | 1985-12-28 | Mta Koezponti Kemiai Kutato In | Herbicide compositions containing antitoxines of aldehyde- and ketone-derivatives |
US4774381A (en) | 1983-09-30 | 1988-09-27 | E. I. Du Pont De Nemours And Company | Herbicide resistant tobacco |
US4761373A (en) | 1984-03-06 | 1988-08-02 | Molecular Genetics, Inc. | Herbicide resistance in plants |
US5331107A (en) | 1984-03-06 | 1994-07-19 | Mgi Pharma, Inc. | Herbicide resistance in plants |
US5304732A (en) | 1984-03-06 | 1994-04-19 | Mgi Pharma, Inc. | Herbicide resistance in plants |
EP0154204B1 (en) | 1984-03-06 | 1994-01-12 | Mgi Pharma, Inc. | Herbicide resistance in plants |
US6211439B1 (en) | 1984-08-10 | 2001-04-03 | Mgi Pharma, Inc | Herbicide resistance in plants |
US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
US5605011A (en) | 1986-08-26 | 1997-02-25 | E. I. Du Pont De Nemours And Company | Nucleic acid fragment encoding herbicide resistant plant acetolactate synthase |
US5013659A (en) | 1987-07-27 | 1991-05-07 | E. I. Du Pont De Nemours And Company | Nucleic acid fragment encoding herbicide resistant plant acetolactate synthase |
IL83348A (en) | 1986-08-26 | 1995-12-08 | Du Pont | Nucleic acid fragment encoding herbicide resistant plant acetolactate synthase |
US5378824A (en) | 1986-08-26 | 1995-01-03 | E. I. Du Pont De Nemours And Company | Nucleic acid fragment encoding herbicide resistant plant acetolactate synthase |
US5821126A (en) | 1986-11-19 | 1998-10-13 | The Regents Of The University Of California | Method for clonal propagation of gymnosperms by somatic polyembryogenesis |
CA1335412C (en) | 1987-03-27 | 1995-05-02 | Eric B. Swanson | Microspore-based selection system and products thereof |
PH24574A (en) | 1987-06-01 | 1990-08-03 | Hokko Chem Ind Co | Method of breeding rice plants of new variety having resistance to herbicides |
IE61148B1 (en) | 1988-03-10 | 1994-10-05 | Ici Plc | Method of detecting nucleotide sequences |
FR2629098B1 (en) * | 1988-03-23 | 1990-08-10 | Rhone Poulenc Agrochimie | CHEMICAL GENE OF HERBICIDE RESISTANCE |
US5858652A (en) | 1988-08-30 | 1999-01-12 | Abbott Laboratories | Detection and amplification of target nucleic acid sequences |
US5084082A (en) | 1988-09-22 | 1992-01-28 | E. I. Du Pont De Nemours And Company | Soybean plants with dominant selectable trait for herbicide resistance |
EP0360750A3 (en) | 1988-09-22 | 1991-01-02 | Ciba-Geigy Ag | Novel herbicide tolerant plants |
GB8825402D0 (en) | 1988-10-31 | 1988-11-30 | Cambridge Advanced Tech | Sulfonamide resistance genes |
EP0375875A3 (en) | 1988-12-30 | 1991-01-09 | American Cyanamid Company | A method to improve the protection of crops from herbicidal injury |
ES2080826T3 (en) | 1989-05-17 | 1996-02-16 | Zeneca Ltd | HERBICIDE-RESISTANT CORN. |
ATE129745T1 (en) | 1990-03-02 | 1995-11-15 | Biocem | REGENERATION AND GENETIC TRANSFORMATION OF SUGAR BEET. |
DE69126474T2 (en) | 1990-05-09 | 1997-12-11 | American Cyanamid Co | Process to prevent crop damage caused by synergistic combinations of pesticides |
US5198599A (en) | 1990-06-05 | 1993-03-30 | Idaho Resarch Foundation, Inc. | Sulfonylurea herbicide resistance in plants |
GB9024728D0 (en) | 1990-11-14 | 1991-01-02 | Ici Plc | Herbicide resistant plants |
DE4038430A1 (en) | 1990-12-01 | 1992-06-04 | Basf Ag | HERBICIDES N - ((1,3,5-TRIAZIN-2-YL) -AMINOCARBONYL) BENZOLSULFONAMIDE |
US5767366A (en) | 1991-02-19 | 1998-06-16 | Louisiana State University Board Of Supervisors, A Governing Body Of Louisiana State University Agricultural And Mechanical College | Mutant acetolactate synthase gene from Ararbidopsis thaliana for conferring imidazolinone resistance to crop plants |
ATE493879T1 (en) | 1991-04-08 | 2011-01-15 | Basf Se | WHEAT RESISTANT TO AHAS-INHIBITING HERBICIDE AND METHOD FOR SELECTION THEREOF |
GB9114259D0 (en) * | 1991-07-02 | 1991-08-21 | Ici Plc | Plant derived enzyme and dna sequences |
US5731180A (en) | 1991-07-31 | 1998-03-24 | American Cyanamid Company | Imidazolinone resistant AHAS mutants |
DE4206146A1 (en) | 1992-02-28 | 1993-09-02 | Basf Ag | HERBICIDES N - ((1,3,5-TRIAZIN-2-YL) AMINOCARBONYL) BENZOLSULFONAMIDE |
US5545822A (en) | 1992-08-21 | 1996-08-13 | Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College | Herbicide resistant rice |
FR2696190B1 (en) | 1992-09-25 | 1994-12-09 | Agronomique Inst Nat Rech | Nucleic acid coding for an alpha-acetolactate synthase and its applications. |
US5539092A (en) | 1992-10-02 | 1996-07-23 | Arch Development Corporation | Cyanobacterial and plant acetyl-CoA carboxylase |
DE4238175A1 (en) | 1992-11-12 | 1994-05-19 | Basf Ag | Herbicides Sulfonylureas, process for their preparation and their use |
JP3339655B2 (en) | 1993-10-04 | 2002-10-28 | 花王株式会社 | Catalyst precursor for hydrogenation reaction, method for producing the same, and method for producing alcohol |
US5633437A (en) | 1994-10-11 | 1997-05-27 | Sandoz Ltd. | Gene exhibiting resistance to acetolactate synthase inhibitor herbicides |
US5853973A (en) | 1995-04-20 | 1998-12-29 | American Cyanamid Company | Structure based designed herbicide resistant products |
CZ331797A3 (en) | 1995-04-20 | 1998-06-17 | American Cyanamid Company | Products resistant to herbicides developed on a structure |
DE19518505A1 (en) | 1995-05-19 | 1996-11-21 | Max Planck Gesellschaft | Procedure for gene expression analysis |
US5773704A (en) | 1996-04-29 | 1998-06-30 | Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College | Herbicide resistant rice |
US5773702A (en) | 1996-07-17 | 1998-06-30 | Board Of Trustees Operating Michigan State University | Imidazolinone herbicide resistant sugar beet plants |
WO1998002527A1 (en) | 1996-07-17 | 1998-01-22 | Michigan State University | Imidazolinone herbicide resistant sugar beet plants |
US5859348A (en) | 1996-07-17 | 1999-01-12 | Board Of Trustees Operating Michigan State University | Imidazolinone and sulfonyl urea herbicide resistant sugar beet plants |
US6358686B1 (en) | 1996-12-02 | 2002-03-19 | Affymetrix, Inc. | Brassica polymorphisms |
US6114116A (en) | 1996-12-02 | 2000-09-05 | Lemieux; Bertrand | Brassica polymorphisms |
AU6024598A (en) | 1997-01-10 | 1998-08-03 | Pioneer Hi-Bred International, Inc. | Hybridization-based genetic amplification and analysis |
DE69823017T2 (en) | 1997-01-29 | 2004-09-16 | Corning S.A. | Lenses with a very high refractive index |
US6207425B1 (en) | 1997-09-11 | 2001-03-27 | City Of Hope | Bidirectional PCR amplification of specific alleles |
GB9807818D0 (en) | 1998-04-09 | 1998-06-10 | Zeneca Ltd | Improvements in or relating to organic compounds |
BR9911313A (en) | 1998-06-16 | 2001-03-13 | Basf Ag | Synergistically herbicidal mixture, herbicidal composition, and processes for preparing herbicidal compositions and for controlling unwanted vegetation |
AU4956399A (en) | 1998-06-19 | 2000-01-05 | Calvin F. Konzak | Methods for generating and identifying mutant polyploid plants, and uses therefor |
US6696294B1 (en) | 1998-06-19 | 2004-02-24 | Northwest Plant Breeding Co. | Methods for generating and identifying mutant polyploid plants, and uses therefor |
US6348643B1 (en) | 1998-10-29 | 2002-02-19 | American Cyanamid Company | DNA sequences encoding the arabidopsis acetohydroxy-acid synthase small subunit and methods of use |
US7019196B1 (en) | 1998-11-05 | 2006-03-28 | Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College | Herbicide resistant rice |
CN1330511A (en) | 1998-11-05 | 2002-01-09 | 路易斯安那州州立大学及农业机械学院管理委员会 | Herbicid resistant rice |
EP1033405A3 (en) | 1999-02-25 | 2001-08-01 | Ceres Incorporated | Sequence-determined DNA fragments and corresponding polypeptides encoded thereby |
AU3514700A (en) | 1999-03-08 | 2000-09-28 | Pioneer Hi-Bred International, Inc. | Maize metallothionein gene and promoter |
US6175065B1 (en) | 1999-04-14 | 2001-01-16 | Pioneer Hi-Bred International, Inc. | Inbred sunflower line PHA344 |
GB9917307D0 (en) | 1999-07-23 | 1999-09-22 | Sec Dep Of The Home Department | Improvements in and relating to analysis of DNA |
WO2001021821A2 (en) | 1999-09-17 | 2001-03-29 | Aventis Cropscience N.V. | Insect-resistant rice plants |
US20010044939A1 (en) | 2000-01-04 | 2001-11-22 | Abell Lynn M. | Small subunit of plant acetolactate synthase |
US6613963B1 (en) | 2000-03-10 | 2003-09-02 | Pioneer Hi-Bred International, Inc. | Herbicide tolerant Brassica juncea and method of production |
US6936467B2 (en) | 2000-03-27 | 2005-08-30 | University Of Delaware | Targeted chromosomal genomic alterations with modified single stranded oligonucleotides |
CA2407396C (en) | 2000-04-28 | 2013-12-31 | Basf Aktiengesellschaft | Use of the maize x112 mutant ahas 2 gene and imidazolinone herbicides for selection of transgenic monocots |
AU2000246809A1 (en) | 2000-04-28 | 2001-11-12 | Aventis Cropscience N.V. | Glufosinate tolerant rice |
ES2608927T3 (en) | 2000-05-10 | 2017-04-17 | Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College | Resistance to herbicides that inhibit acetohydroxy acid synthase |
US6475736B1 (en) | 2000-05-23 | 2002-11-05 | Variagenics, Inc. | Methods for genetic analysis of DNA using biased amplification of polymorphic sites |
ATE447011T1 (en) | 2000-06-27 | 2009-11-15 | Basf Se | NUCLEIC ACID FRAGMENTS FROM CYANOBACTERIA WHICH CODE FOR PROTEINS THAT CONTROL PROPERTIES OF PLANTS THROUGH NUCLEAR OR PLASTOMER TRANSFORMATION |
AU2001282984A1 (en) | 2000-07-26 | 2002-02-05 | Wisys Technology Foundation, Inc. | Method and apparatus for producing an electrical property image using a charge correlation matrix |
CA2326283C (en) | 2000-11-17 | 2008-04-29 | Pioneer Hi-Bred International, Inc. | Brassica napus with early maturity (early napus) and resistance to an ahas-inhibitor herbicide |
CA2326285C (en) | 2000-11-17 | 2008-05-06 | Pioneer Hi-Bred International, Inc. | Brassica with resistance to an ahas-inhibitor herbicide and blackleg disease |
US20030097692A1 (en) | 2000-12-21 | 2003-05-22 | Georg Jander | Plants with imidazolinone-resistant ALS |
US6627401B2 (en) | 2000-12-28 | 2003-09-30 | Council Of Scientific And Industrial Research | Method for detecting a single nucleotide polymorphism in p21waf1/cip1 gene as an indicator of risk of esophageal cancer |
US6492582B2 (en) | 2001-01-11 | 2002-12-10 | California Cooperative Rice Research Foundation, Inc. | Rice cultivar M-205 |
TR200301979T2 (en) | 2001-05-14 | 2004-11-22 | University Of Saskatchewan | Lentil plants with increased resistance to imidazolinone herbicides |
GB0118928D0 (en) | 2001-08-02 | 2001-09-26 | Syngenta Participations Ag | DNA molecules conferring tolerance to herbicidal compounds |
CA2456311C (en) | 2001-08-09 | 2014-04-08 | University Of Saskatchewan | Wheat plants having increased resistance to imidazolinone herbicides |
BR0211610A (en) | 2001-08-09 | 2006-04-04 | Northwest Plant Breeding Compa | wheat seedlings with higher resistance to imidazolinone herbicides |
RU2337531C2 (en) | 2001-08-09 | 2008-11-10 | Юниверсити Оф Саскачеван | Wheat plants with higher resistance to imidazolinone herbicides |
WO2003020983A1 (en) | 2001-08-30 | 2003-03-13 | Virginia Commonwealth University | Allele specific pcr for genotyping |
WO2003048319A2 (en) | 2001-11-30 | 2003-06-12 | Syngenta Participations Ag | Nucleotide sequences encoding proteins conferring abiotic stress tolerance in plants |
US20040023262A1 (en) | 2002-03-05 | 2004-02-05 | Pioneer Hi-Bred International, Inc. | Targeted manipulation of genes in plants |
US7829762B2 (en) | 2002-07-10 | 2010-11-09 | Department Of Agriculture | Wheat plants having increased resistance to imidazolinone herbicides |
EP1556491A4 (en) | 2002-07-17 | 2007-02-07 | Sepro Corp | Herbicide-resistant plants, and polynucleotides and methods for providing same |
US7094951B2 (en) | 2002-09-09 | 2006-08-22 | The Ohio State University | Cloning and characterization of the broad-spectrum resistance gene Pi2 |
EP1558767A2 (en) | 2002-10-29 | 2005-08-03 | BASF Plant Science GmbH | Compositions and methods for identifying plants having increased tolerance to imidazolinone herbicides |
DE60335645D1 (en) | 2002-10-29 | 2011-02-17 | Advanta Canada Inc | ASSAY FOR IMIDAZOLINONE RESISTANCE MUTATIONS IN BRASSICA SPECIES |
US6953880B2 (en) | 2003-02-28 | 2005-10-11 | University Of Arkansas, N.A. | Rice cultivar ‘Francis’ |
AU2004243416B2 (en) | 2003-05-28 | 2009-06-11 | Basf Se | Wheat plants having increased tolerance to imidazolinone herbicides |
UY28495A1 (en) | 2003-08-29 | 2005-03-31 | Inst Nac De Tecnologia Agropec | RICE PLANTS THAT HAVE A GREATER TOLERANCE TO IMIDAZOLINONA HERBICIDES |
US7432082B2 (en) | 2004-03-22 | 2008-10-07 | Basf Ag | Methods and compositions for analyzing AHASL genes |
MXPA06014761A (en) | 2004-06-16 | 2007-03-26 | Basf Plant Science Gmbh | Polynucleotides encoding mature ahasl proteins for creating imidazolinone-tolerant plants. |
US7355098B2 (en) | 2004-06-22 | 2008-04-08 | Saskatchewan Wheat Poo1 | Brassica AHAS genes and gene alleles that provide resistance to imidazolinone herbicides |
AR051690A1 (en) | 2004-12-01 | 2007-01-31 | Basf Agrochemical Products Bv | MUTATION INVOLVED IN THE INCREASE OF TOLERANCE TO IMIDAZOLINONE HERBICIDES IN PLANTS |
ES2692594T1 (en) | 2005-03-02 | 2018-12-04 | Instituto Nacional De Tecnologia Agropecuaria | Rice plants resistant to herbicides, polynucleotides that encode herbicide-resistant proteins of the large subunit of acetohydroxy acid synthase and methods for their use |
EP1902136B1 (en) | 2005-07-01 | 2012-01-18 | Basf Se | Herbicide-resistant sunflower plants, polynucleotides encoding herbicide-resistant acetohydroxyacid synthase large subunit proteins, and methods of use |
US7786360B2 (en) | 2005-09-09 | 2010-08-31 | Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College | Rice cultivar designated ‘CL131’ |
US20070118920A1 (en) | 2005-11-09 | 2007-05-24 | Basf Agrochemical Products B.V. | Herbicide-resistant sunflower plants, polynucleotides encoding herbicide-resistant acetohydroxyacid synthase large subunit proteins, and methods of use |
CL2007001552A1 (en) | 2006-05-31 | 2008-01-18 | Basf Agrochemical Products Bv | Method for producing wheat plant with high protein content that includes introducing the ahasl1a s653n gene, method of generating wheat flour with high protein content, and food product that comprises it. |
UA108733C2 (en) * | 2006-12-12 | 2015-06-10 | Sunflower herbicide tolerant to herbicide | |
US20100287641A1 (en) | 2007-04-04 | 2010-11-11 | Basf Plant Science Gmbh | Ahas mutants |
US7642434B2 (en) | 2007-05-02 | 2010-01-05 | Board Of Trustees Of The University Of Arkansas, N.A. | Rice cultivar CL171-AR |
EA026107B1 (en) | 2007-10-05 | 2017-03-31 | СИБАС ЕУРОП Би.Ви. | Acetohydroxyacid synthase genes in brassica plant comprising mutations and use thereof |
-
2007
- 2007-11-12 UA UAA200906898A patent/UA108733C2/en unknown
- 2007-12-11 AU AU2007331535A patent/AU2007331535A1/en not_active Abandoned
- 2007-12-11 HU HUE07848061A patent/HUE029584T2/en unknown
- 2007-12-11 WO PCT/EP2007/063737 patent/WO2008071715A1/en active Application Filing
- 2007-12-11 CA CA002672227A patent/CA2672227A1/en not_active Abandoned
- 2007-12-11 EP EP07848061.3A patent/EP2091319B1/en active Active
- 2007-12-11 US US12/517,919 patent/US9035133B2/en not_active Expired - Fee Related
- 2007-12-11 CL CL2007003605A patent/CL2007003605A1/en unknown
- 2007-12-11 EA EA200900768A patent/EA019478B1/en not_active IP Right Cessation
- 2007-12-11 BR BRPI0720338-1A2A patent/BRPI0720338A2/en not_active IP Right Cessation
- 2007-12-11 CN CN200780049043.7A patent/CN101657090B/en not_active Expired - Fee Related
- 2007-12-11 JP JP2009540756A patent/JP2010512157A/en active Pending
- 2007-12-11 ES ES07848061.3T patent/ES2581987T3/en active Active
- 2007-12-12 UY UY30781A patent/UY30781A1/en unknown
- 2007-12-12 AR ARP070105560A patent/AR064298A1/en unknown
-
2009
- 2009-07-09 ZA ZA2009/04820A patent/ZA200904820B/en unknown
-
2015
- 2015-01-27 US US14/606,219 patent/US20150211017A1/en not_active Abandoned
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1999019493A2 (en) * | 1997-10-10 | 1999-04-22 | North Carolina State University | Cytochrome p-450 constructs and methods of producing herbicide-resistant transgenic plants |
WO2001065922A2 (en) * | 2000-03-09 | 2001-09-13 | E. I. Du Pont De Nemours And Company | Sulfonylurea-tolerant sunflower plants |
WO2006024351A1 (en) * | 2004-07-30 | 2006-03-09 | Basf Agrochemical Products B.V. | Herbicide-resistant sunflower plants, plynucleotides encoding herbicide-resistant acetohydroxy acid synthase large subunit proteins, and methods of use |
Non-Patent Citations (9)
Title |
---|
AL-KHATIB K ET AL: "IMAZETHAPYR RESISTANCE IN COMMON SUNFLOWER (HELIANTHUS ANNUUS)", WEED SCIENCE, WEED SCIENCE SOCIETY OF AMERICA, CHAMPAIGN, IL, US, vol. 46, no. 4, 1998, pages 403 - 407, XP001024427, ISSN: 0043-1745 * |
GANG PAN ET AL: "Map-based cloning of a novel rice cytochrome P450 gene CYP81A6 that confers resistance to two different classes of herbicides", PLANT MOLECULAR BIOLOGY, KLUWER ACADEMIC PUBLISHERS, DO, vol. 61, no. 6, 1 August 2006 (2006-08-01), pages 933 - 943, XP019405487, ISSN: 1573-5028 * |
INUI HIDEYUKI ET AL: "Herbicide resistance in transgenic plants with mammalian P450 monooxygenase genes", PEST MANAGEMENT SCIENCE, vol. 61, no. 3, March 2005 (2005-03-01), pages 286 - 291, XP002471263, ISSN: 1526-498X * |
KOLKMAN J M ET AL: "Acetohydroxyacid synthase mutations conferring resistance to imidazolinone or sulfonylurea herbicides in sunflower", THEORETICAL AND APPLIED GENETICS, SPRINGER, BERLIN, DE, vol. 109, no. 6, October 2004 (2004-10-01), pages 1147 - 1159, XP002417954, ISSN: 0040-5752 * |
TAN S ET AL: "Herbicidal inhibitors of amino acid biosynthesis and herbicide-tolerant crops", AMINO ACIDS, SPRINGER VERLAG, AU, vol. 30, no. 2, March 2006 (2006-03-01), pages 195 - 204, XP002455167, ISSN: 0939-4451 * |
TAN S ET AL: "IMIDAZOLINONE-TOLERANT CROPS: HISTORY, CURRENT STATUS AND FUTURE", PEST MANAGEMENT SCIENCE, WILEY & SONS, BOGNOR REGIS, GB, vol. 61, no. 3, 2005, pages 246 - 257, XP009058795, ISSN: 1526-498X * |
WERCK-REICHHART D ET AL: "Cytochromes P450 for engineering herbicide tolerance", TRENDS IN PLANT SCIENCE, ELSEVIER SCIENCE, OXFORD, GB, vol. 5, no. 3, March 2000 (2000-03-01), pages 116 - 123, XP002344704, ISSN: 1360-1385 * |
YU QIN ET AL: "Tolerance to acetolactate synthase and acetyl-coenzyme A carboxylase inhibiting herbicides in Vulpia bromoides is conferred by two co-existing resistance mechanisms.", PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY, vol. 78, no. 1, January 2004 (2004-01-01), pages 21 - 30, XP002471262, ISSN: 0048-3575 * |
ZELAYA I A ET AL: "EVOLVED RESISTANCE TO ACETOLACTATE SYNTHASE-INHIBITING HERBICIDES IN COMMON SUNFLOWER (HELIANTHUS ANNUUS), GIANT RAGWEED (AMBROSIA TRIFIDA), AND SHATTERCANE (SORGHUM BICOLOR) IN IOWA", WEED SCIENCE, WEED SCIENCE SOCIETY OF AMERICA, CHAMPAIGN, IL, US, vol. 52, no. 4, July 2004 (2004-07-01), pages 538 - 548, XP009058787, ISSN: 0043-1745 * |
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JP2013514791A (en) * | 2009-12-22 | 2013-05-02 | バイエル・クロップサイエンス・エヌ・ヴェー | Herbicide-tolerant plants |
WO2013004281A1 (en) | 2011-07-01 | 2013-01-10 | Advanta International Bv | Nucleotide sequences mutated by insertion that encode a truncated oleate desaturase protein, proteins, methods and uses |
WO2013004280A1 (en) | 2011-07-01 | 2013-01-10 | Advanta International Bv | Isolated mutated nucleotide sequences that encode a modified oleate destaurase sunflower protein, modified protein, methods and uses |
EP4303306A2 (en) | 2011-07-01 | 2024-01-10 | Advanta Holdings BV | Nucleotide sequences mutated by insertion that encode a truncated oleate desaturase protein, proteins, methods and uses |
Also Published As
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ZA200904820B (en) | 2012-06-27 |
CA2672227A1 (en) | 2008-06-19 |
UY30781A1 (en) | 2008-07-03 |
CL2007003605A1 (en) | 2009-01-23 |
EA200900768A1 (en) | 2010-02-26 |
US20150211017A1 (en) | 2015-07-30 |
US20120023601A1 (en) | 2012-01-26 |
CN101657090A (en) | 2010-02-24 |
UA108733C2 (en) | 2015-06-10 |
EP2091319B1 (en) | 2016-04-13 |
EA019478B1 (en) | 2014-04-30 |
CN101657090B (en) | 2015-04-15 |
ES2581987T3 (en) | 2016-09-08 |
US9035133B2 (en) | 2015-05-19 |
AU2007331535A1 (en) | 2008-06-19 |
AR064298A1 (en) | 2009-03-25 |
EP2091319A1 (en) | 2009-08-26 |
BRPI0720338A2 (en) | 2013-12-24 |
JP2010512157A (en) | 2010-04-22 |
HUE029584T2 (en) | 2017-03-28 |
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