WO2008070599A1

WO2008070599A1 - Indazole compounds

Info

Publication number: WO2008070599A1
Application number: PCT/US2007/086220
Authority: WO
Inventors: Chung-Ming Sun; Min-Liang Kuo
Original assignee: Chung-Ming Sun; Min-Liang Kuo
Priority date: 2006-12-05
Filing date: 2007-12-03
Publication date: 2008-06-12
Also published as: US7825261B2; TWI433672B; EP2091329A4; AU2007329480A1; EP2091329A1; CN101616587A; US20080132501A1; KR20090127867A; TW200836720A; JP2010511721A; CA2671543A1

Abstract

This invention relates to indazole compounds of formula (I) or (II) shown below. Each variable in formula (I) or (II) is defined in the specification. These compounds can be used to treat cancer.

Description

Indazole Compounds

CROSS REFERENCETO RELATEDAPPLICATION

This application claims priority to U.S. Provisional Application Serial No. 60/873,041, filed December 05, 2006, the content of which is incorporated herein by reference.

BACKGROUND

The role of lymphangiogenesis in promoting metastasis via the lymphatic system has been the subject of extensive research. Vascular endothelial growth factor receptor-3 (VEGFR-3) is a major mediator of lymphangiogenesis. VEGF-C and VEGF-D are two ligands for VEGFR-3. Both of them were shown to stimulate lymphangiogenesis in transgenic mice. Specifically, three cancer cell lines transfected with VEGF-C or

VEGF-D were recently reported to exhibit increased tumor lymphangiogenesis and undergo lymphatic metastasis. Clinical studies also revealed that increased expression of VEGF-C was associated with lymph node metastasis in a variety of cancers in human. Thus, it is desirable to develop novel drugs that inhibit VEGFR-3 activities for use in treating cancer.

SUMMARY

This invention is based on the discovery that certain indazole compounds are effective in reducing metastasis and treating cancer by inhibiting VEGFR-3 activities. In one aspect, this invention features indazole compounds of formula (I):

In this formula, each ₌₌₌₁ independently is a double bond or single bond; each of X₁, X₂, X₃, X₄, X₅, X₆, and X₇, independently, is C or N, provided that at least two of X₁, X₂, and X₃ are N; and that when X₁ is N, R₁ is deleted, when X₃ is N, R₃ is deleted, i when X₄ is N, R₄ is deleted, when X₅ is N, R₅ is deleted, when X₆ is N, R₆ is deleted, and when X₇ is N, R₇ is deleted; each of Xs and Xg, independently, is C or N⁺; and each of R₁, R₂, R₃, R₄, R₅, R₆, and R₇, independently, is H, C₁-C_1O alkyl, C₂-Ci₀ alkenyl, C₂-CiO alkynyl, C₃-C₂₀ cycloalkyl, C₃-C₂₀ cycloalkenyl, Ci-C₂₀ heterocycloalkyl, Ci-C₂₀ heterocycloalkenyl, aryl, heteroaryl, halo, CN, NO₂, OR_a, COOR₃, OC(O)R₃, C(O)R₃, C(0)NR_aR_b, C(0)N(R_a)N(R_b)C(0)R_c, NR_aR_b, N(Rc)SO₂NR₃Rb, S0₂NR_aR_b, or SR₃, in which each of R₃, R_b, and R_c, independently, is H, Ci-Cio alkyl, C₃-C₂₀ cycloalkyl, Ci-C₂₀ heterocycloalkyl, aryl, or heteroaryl, or R₃ and R_b together with the nitrogen atom to which they are attached form a C1-C2₀ heterocycloalkyl or heteroaryl.

Referring to formula (I), a subset of the indazole compounds described above are those in which each of X₁, X₄, X₅, X₆, X₇, Xs, and X₉, independently, is C and each of X₂ and X₃, independently, is N. In these compounds, R₁ can be H or OR₃; R₂ can be C₃-C_2O cycloalkyl, CrCi₀ alkyl optionally substituted with aryl or Ci-C₂₀ heterocycloalkyl, or aryl optionally substituted with C₁-C₁₀ alkyl; and each of R₄, R₅, R₆, and R₇, independently, can be H, Ci -C₁₀ alkyl, NR_aR_b, COOR₃, C(0)NR_aR_b, C(0)N(R_a)N(R_b)C(0)R_c, or heteroaryl.

In another aspect, this invention features indazole compounds of formula (II):

In this formula, each of X₁, X₂, and X₃, independently, is C or N, provided that at least two of Xi, X₂, and X₃ are N; and that when Xi is N, Ri is deleted, when X₂ is N, R₂ is deleted; and each of R₁, R₂, R₃, R₄, R5, R₆, and R₇, independently, is H, Ci-Ci₀ alkyl, C₂-Ci₀ alkenyl, C₂-Ci₀ alkynyl, C₃-C₂₀ cycloalkyl, C₃-C₂₀ cycloalkenyl, Ci-C₂₀ heterocycloalkyl, Ci-C₂₀ heterocycloalkenyl, aryl, heteroaryl, halo, CN, NO₂, 0R_a, COOR₃, OC(O)R₃, C(O)R₃, C(O)NR₃R₅, C(0)N(R₃)N(R_b)C(0)R_c, NR_aR_b,

N(R_c)SO₂NR₃R_b, S0₂NR_aR_b, or SR₃, in which each of R₃, R_b, and R_c, independently, is H, Ci-Cio alkyl, C₃-C₂₀ cycloalkyl, Ci-C_2O heterocycloalkyl, aryl, or heteroaryl, or R₃ and Rb together with the nitrogen atom to which they are attached form a C₁-C_2O heterocycloalkyl or heteroaryl.

Referring to formula (II), a subset of the indazole compounds described above are those in which X₁ is C, each of X₂ and X₃, independently, is N, and each of R₃, R₄, R₅, R₆, and R₇, independently, is H, C₁-C₁₀ alkyl, NR_aR_b, COOR₃, C(O)NR_aR_b, C(0)N(R_a)N(R_b)C(0)R_c, or heteroaryl.

The term "compound" used herein includes both compounds and ions. For example, when Xs or X₉ is N⁺, the compound of formula (I) is a cation. The term "alkyl" refers to a saturated, linear or branched hydrocarbon moiety, such as -CH₃ or - CH(CH₃)₂. The term "alkenyl" refers to a linear or branched hydrocarbon moiety that contains at least one double bond, such as -CH=CH-CH₃. The term "alkynyl" refers to a linear or branched hydrocarbon moiety that contains at least one triple bond, such as -C≡C-CH₃. The term "cycloalkyl" refers to a saturated, cyclic hydrocarbon moiety, such as cyclohexyl. The term "cycloalkenyl" refers to a non-aromatic, cyclic hydrocarbon moiety that contains at least one double bond, such as cyclohexenyl. The term "heterocycloalkyl" refers to a saturated, cyclic moiety having at least one ring heteroatom (e.g., N, O, or S), such as 4-tetrahydropyranyl. The term "heterocycloalkenyl" refers to a non-aromatic, cyclic moiety having at least one ring heteroatom (e.g., N, O, or S) and at least one ring double bond, such as pyranyl. The term "aryl" refers to a hydrocarbon moiety having one or more aromatic rings.

Examples of aryl moieties include phenyl (Ph), phenylene, naphthyl, naphthylene, pyrenyl, anthryl, and phenanthryl. The term "heteroaryl" refers to a moiety having one or more aromatic rings that contain at least one heteroatom (e.g., N, O, or S). Examples of heteroaryl moieties include furyl, furylene, fluorenyl, pyrrolyl, thienyl, oxazolyl, imidazolyl, thiazolyl, pyridyl, pyrimidinyl, quinazolinyl, quinolyl, isoquinolyl and indolyl.

Alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkenyl, heterocycloalkyl, heterocycloalkenyl, aryl, and heteroaryl mentioned herein include both substituted and unsubstituted moieties, unless specified otherwise. Possible substituents on cycloalkyl, cycloalkenyl, heterocycloalkyl, heterocycloalkenyl, aryl, and heteroaryl include, but are not limited to, C₁-C_1O alkyl, C₂-C₁O alkenyl, C₂-C₁O alkynyl, C₃-C₂O cycloalkyl, C₃-C₂Q cycloalkenyl, C₁-C₂Q heterocycloalkyl, C₁-C_2O heterocycloalkenyl, Ci-C₁O alkoxy, aryl, aryloxy, heteroaryl, heteroaryloxy, amino, Ci-C_1O alkylamino, C₁- C₂o dialkylamino, arylamino, diarylamino, CrC_1O alkylsulfonamino, arylsulfonamino, Ci-Cio alkylimino, arylimino, C₁-C₁O alkylsulfonimino, arylsulfonimino, hydroxyl, halo, thio, C₁-Ci₀ alkylthio, arylthio, C₁-Ci_O alkylsulfonyl, arylsulfonyl, acylamino, aminoacyl, aminothioacyl, amidino, guanidine, ureido, cyano, nitro, nitroso, azido, acyl, thioacyl, acyloxy, carboxyl, and carboxylic ester. On the other hand, possible substituents on alkyl, alkenyl, or alkynyl include all of the above-recited substituents except Ci-Cio alkyl. Cycloalkyl, cycloalkenyl, heterocyclo alkyl, heterocycloalkenyl, aryl, and heteroaryl can also be fused with each other. In another aspect, this invention features a method for treating cancer. The method includes administering to a subject in need thereof an effective amount of one or more indazole compounds of formula (I) or (II) shown above. An example of cancer that can be treated by the indazole compounds of this invention is lung cancer. The term "treating" or "treatment" refers to administering one or more indazole compounds to a subject, who has an above-described disease, a symptom of such a disease, or a predisposition toward such a disease, with the purpose to confer a therapeutic effect, e.g., to cure, relieve, alter, affect, ameliorate, or prevent the above- described disease, the symptom of it, or the predisposition toward it.

In addition, this invention encompasses a pharmaceutical composition that contains at least one of the above-mentioned indazole compounds and a pharmaceutically acceptable carrier.

The indazole compounds described above include the compounds themselves, as well as their salts, prodrugs, and solvates, if applicable. A salt, for example, can be formed between an anion and a positively charged group (e.g., amino) on a indazole compound. Suitable anions include chloride, bromide, iodide, sulfate, nitrate, phosphate, citrate, methanesulfonate, trifluoroacetate, acetate, malate, tosylate, tartrate, fumurate, glutamate, glucuronate, lactate, glutarate, and maleate. Likewise, a salt can also be formed between a cation and a negatively charged group (e.g., carboxylate) on a indazole compound. Suitable cations include sodium ion, potassium ion, magnesium ion, calcium ion, and an ammonium cation such as tetramethyl ammonium ion. The indazole compounds also include those salts containing quaternary nitrogen atoms. Examples of prodrugs include esters and other pharmaceutically acceptable derivatives, which, upon administration to a subject, are capable of providing active indazole compounds. A solvate refers to a complex formed between an active indazole compound and a pharmaceutically acceptable solvent. Examples of pharmaceutically acceptable solvents include water, ethanol, isopropanol, ethyl acetate, acetic acid, and ethanolamine.

Also within the scope of this invention is a composition containing one or more of the indazole compounds described above for use in treating cancer, and the use of such a composition for the manufacture of a medicament for the just-mentioned treatment. The details of one or more embodiments of the invention are set forth in the description below. Other features, objects, and advantages of the invention will be apparent from the description and from the claims.

DETAILED DESCRIPTION

Shown below are 55 exemplary compounds of this invention:

Compound 5 Compound 6

Compound 8 Compound 9

Compound 10 Compound 11 Compound 12

Compound 15

Compound 16 Compound 17 Compound 18

Compound 19 Compound 20

Compound 30 Compound 31 Compound 32

Compound 33 Compound 34 Compound 35

Compound 36 Compound 37

Compound 38 Compound 39 Compound 40

Compound 41 Compound 42 Compound 43

Compound 44 Compound 45

Compound 46 Compound 47 Compound 48

OH

Compound 49 Compound 50 Compound 51

Compound 52 Compound 53

Compound 54 Compound 55 The indazole compounds described above can be prepared by methods well known in the art. Examples 1-55 below provide detailed descriptions of how compounds 1-55 were actually prepared.

Scheme I shown below illustrates a typical synthetic route for synthesizing certain exemplary indazole compounds. R₂ and R₅ in this scheme can be those described in the Summary section above.

Scheme I

R = halo )

Specifically, as shown in Scheme I above, a substituted benzene containing a nitro group and a halo group can first react with a primary amine compound to form a secondary amine compound. This compound can then undergo a ring closure reaction between the nitro group and the secondary amino group to form an indazole compound of this invention. An indazole compound synthesized above can be purified by a suitable method such as column chromatography, high-pressure liquid chromatography, or recrystallization.

Other indazole compounds can be prepared using other suitable starting materials through the above synthetic routes and others known in the art. The methods described above may also additionally include steps, either before or after the steps described specifically herein, to add or remove suitable protecting groups in order to ultimately allow synthesis of the indazole compounds. In addition, various synthetic steps may be performed in an alternate sequence or order to give the desired compounds. Synthetic chemistry transformations and protecting group methodologies (protection and deprotection) useful in synthesizing applicable indazole compounds are known in the art and include, for example, those described in R. Larock, Comprehensive Organic Transformations, VCH Publishers (1989); T.W. Greene and P. G. M. Wuts, Protective Groups in Organic Synthesis, 2^nd Ed., John Wiley and Sons (1991); L. Fieser and M. Fieser, Fieser andFieser's Reagents for Organic Synthesis, John Wiley and Sons (1994); and L. Paquette, ed., Encyclopedia of Reagents for Organic Synthesis, John Wiley and Sons (1995) and subsequent editions thereof.

The indazole compounds mentioned herein may contain a non-aromatic double bond and one or more asymmetric centers. Thus, they can occur as racemates and racemic mixtures, single enantiomers, individual diastereomers, diastereomeric mixtures, and cis- or trans- isomeric forms. All such isomeric forms are contemplated.

Also within the scope of this invention is a pharmaceutical composition containing at least one indazole compound described above and a pharmaceutical acceptable carrier. Further, this invention covers a method of administering an effective amount of one or more of the indazole compounds to a patient having cancer. "An effective amount" refers to the amount of an active indazole compound that is required to confer a therapeutic effect on the treated subject. Effective doses will vary, as recognized by those skilled in the art, depending on the types of diseases treated, route of administration, excipient usage, and the possibility of co-usage with other therapeutic treatment. For example, a daily dose of 5 mg/kg of compound 1 can be used reduce metastasis and a daily dose of 50 mg/kg can be used to inhibit tumor growth. To practice the method of the present invention, a composition having one or more indazole compounds can be administered parenterally, orally, nasally, rectally, topically, or buccally. The term "parenteral" as used herein refers to subcutaneous, intracutaneous, intravenous, intramuscular, intraarticular, intraarterial, intrasynovial, intrasternal, intrathecal, intralesional, or intracranial injection, as well as any suitable infusion technique.

A sterile injectable composition can be a solution or suspension in a non-toxic parenterally acceptable diluent or solvent, such as a solution in 1,3-butanediol. Among the acceptable vehicles and solvents that can be employed are mannitol, water, Ringer's solution, and isotonic sodium chloride solution. In addition, fixed oils are conventionally employed as a solvent or suspending medium (e.g., synthetic mono- or diglycerides). Fatty acid, such as oleic acid and its glyceride derivatives are useful in the preparation of injectables, as are natural pharmaceutically acceptable oils, such as olive oil or castor oil, especially in their polyoxyethylated versions. These oil solutions or suspensions can also contain a long chain alcohol diluent or dispersant, carboxymethyl cellulose, or similar dispersing agents. Other commonly used surfactants such as Tweens or Spans or other similar emulsifying agents or bioavailability enhancers which are commonly used in the manufacture of pharmaceutically acceptable solid, liquid, or other dosage forms can also be used for the purpose of formulation.

A composition for oral administration can be any orally acceptable dosage form including capsules, tablets, emulsions and aqueous suspensions, dispersions, and solutions. In the case of tablets, commonly used carriers include lactose and corn starch. Lubricating agents, such as magnesium stearate, are also typically added. For oral administration in a capsule form, useful diluents include lactose and dried corn starch. When aqueous suspensions or emulsions are administered orally, the active ingredient can be suspended or dissolved in an oily phase combined with emulsifying or suspending agents. If desired, certain sweetening, flavoring, or coloring agents can be added.

A nasal aerosol or inhalation composition can be prepared according to techniques well known in the art of pharmaceutical formulation. For example, such a composition can be prepared as a solution in saline, employing benzyl alcohol or other suitable preservatives, absorption promoters to enhance bioavailability, fluorocarbons, and/or other solubilizing or dispersing agents known in the art.

A composition having one or more active indazole compounds can also be administered in the form of suppositories for rectal administration.

The carrier in the pharmaceutical composition must be "acceptable" in the sense that it is compatible with the active ingredient of the composition (and preferably, capable of stabilizing the active ingredient) and not deleterious to the subject to be treated. One or more solubilizing agents can be utilized as pharmaceutical excipients for delivery of an active indazole compound. Examples of other carriers include colloidal silicon oxide, magnesium stearate, cellulose, sodium lauryl sulfate, and D&C Yellow # 10.

The indazole compounds described above can be preliminarily screened for their efficacy in treating above-described diseases by in vitro and in vivo assays (see Examples 56 and 57 below) and then confirmed by clinic trials. Other methods will also be apparent to those of ordinary skill in the art.

The specific examples below are to be construed as merely illustrative, and not limitative of the remainder of the disclosure in any way whatsoever. Without further elaboration, it is believed that one skilled in the art can, based on the description herein, utilize the present invention to its fullest extent.

Example 1: Preparation of Compound 1: methyl 2-(3,3-diphenylpropyl)-2H-indazole- 6-carboxylate

A solution of dicyclohexylcarbodiimide (DCC, 0.95 g, 4.61 mmol, 1.2 equiv) in 10 mL of dichloromethane (DCM) was added dropwise to a stirred mixture of A- bromomethyl-3-nitro-benzoic acid 1 (1.0 g, 3.84 mmol, 1.0 equiv) and A- dimethylaminomethyl pyridine (DMAP) (0.020 g, 0.19 mmol, 0.05 equiv) in 10 mL of dichloromethane-methanol (10%) at room temperature. The mixture was stirred for 6 hours to obtain 4-bromomethyl-3-nitro-benzoic acid methyl ester 2. Dicyclohexyl urea (DCU) thus obtained was removed by filtration and the solvent in the filtered solution was removed under vacuum. The residue was purified by column chromatography using hexane-ethyl acetate (15%) as an eluant to give ester 2 as a light yellow oil.

To a solution of ester 2 (0.91 g, 3.32 mmol, 1.0 equiv) in 10 mL of dichloromethane was added dropwise 3,3-diphenyl-propylamine (1.40 g, 6.64 mmol, 2.0 equiv). The mixture was stirred at room temperature for 8 hours. After the amine salt thus obtained was removed by filtration, the solvent in the filtered solution was removed under vacuum to give crude 4-[(3,3-diphenyl-propylamino)-methyl]-3-nitro- benzoic acid methyl ester 3. The crude product was purified by column chromatography using hexane-ethyl acetate (25%) to give ester 3 as a light brown oil.

4-[(3,3-diphenyl-propylamino)-methyl]-3-nitro-benzoic acid methyl ester 3 (0.81 g, 3.21 mmol) was dissolved in 10 ml of methanol and treated with ammonium formate (1.26 g, 20.02 mmol, 10 equiv) and palladium on carbon (162 mg, 20 %). The mixture was stirred for 1 day at room temperature. After the mixture was then filtered through a small plug of Celite and washed with dichloromethane, the solvent was removed under vacuum to give a crude product. The crude product was purified by column chromatography using hexane-ethyl acetate (25%) to give compound 1, 2- (3,3-diphenyl-propyl)-2H-indazole-6-carboxylic acid methyl ester, as a white solid.

¹K NMR (300 MHz, CDCl₃) δ 8.53 (s, IH), 7.82 (s, IH), 7.75-7.72 (dd, J = 8.7 , 1.2Hz, IH), 7.69-7.66 (dd, J = 8.7 , 0.5Hz, IH), 7.35-7.20 (m, 10H), 4.41 (t, J = 6.9 Hz, 2H), 3.97 (s, 3H), 3.88 (t, J = 7.9 Hz, IH), 2.87-2.80 (q, J = 7.2 Hz, 2H).

¹³C NMR (75 MHz, CDC13) δ 167.56, 148.13, 143.25, 128.68, 127.71, 126.61, 123.65, 123.27, 121.28, 121.18, 120.07, 52.36, 52.13, 48.12, 35.94; IR (cm-1, neat) : 3236, 2948, 1713, 1601, 1443, 1269.

MS (EI): m/z 370 (M⁺). Exact mass calculated for C₂₄H₂₂N₂O₂: m/z 370.1681 Found 370.1681.

Examples 2-55: Preparation of Compounds 2-55

Compounds 2-55 were prepared in a manner similar to that described in Example 1.

Example 56: KIRA-ELISA assay

This assay was performed in two microtiter plates. The first plate was used to culture an adherent cell line expressing the VEGF receptor 3 and to stimulate the receptor with a test compound. The second plate was used to capture the solubilized membrane receptor, which was then probed for phosphotyrosine content with phosphotyrosine-specific antibody.

Specifically, H928 cells (2xlO⁵) in 100 μl medium were added to each well in a flat-bottom 24-well culture plate and cultured overnight at 37°C in 5% CO₂. After the supernatants were removed, the cells were serum-starved for 24 hours. A medium containing a test compound was added into each well and the cell culture was incubated for 30 minutes before it was stimulated by recombinant VEGF-C for 15 minutes. After the supernatants were removed, 100 μl of a lysis buffer were added into each well to lyse the cells and solubilize the VEGFR3. The lysis buffer included 150 mM NaCl containing 50 mM Hepes (Genentech media prep), 0.5% Triton-X 100 (Genentech media prep), 0.01% thimerosol, 30 kIU/ml aprotinin (ICN Biochemicals, Aurora, OH), 1 mM 4-(2-aminoethyl) benzenesulfonyl fluoride hydrochloride (AEBSF; ICN Biochemicals), and

2 mM sodium ortho vanadate. The plate was then put on a plate shaker (Bellco Instruments Vineland, NJ) and the substance in each well of the plate underwent mixing for 60 minutes at room temperature. While the cells were being solubilized, an ELISA microtiter plate (Nunc Maxisorp, Inter Med, Denmark) coated overnight at 4⁰C with the affinity-purified polyclonal anti-VEGFR 3 (2.5 μg/ml in phosphate buffered saline (PBS), 100 μl/well) were decanted, tamped on a paper towel, and blocked with 150 μl/well block buffer (PBS containing 0.5% BSA and 0.01% thimerosol) for 60 minutes at room temperature with gentle agitation. The anti- VEGFR 3-coated plate was subsequently washed twice with a wash buffer (PBS containing 0.05% Tween 20 and 0.01% thimerosol). The lysate containing solubilized VEGFR 3 from the cell-culture microtiter well were transferred (85 μl / well) to the anti-VEGFR 3-coated ELISA plate and incubated for 2 hours at room temperature with gentle agitation. The unbound receptors were removed by washing with a wash buffer. 100 μl of biotinylated 4G10 (antiphosphotyrosine) diluted to 0.2 μg/ml in dilution buffer (PBS containing 0.5% BSA, 0.05% Tween 20, 5 mM EDTA, and 0.01% thimerosol) were added into each well. After incubation for 2 hours at room temperature, the plate were washed and 100 μl HRP-conjugated streptavidin (Zymed Laboratories, S. San Francisco, CA) diluted 1:2000 in dilution buffer will be further added. After the free avidin conjugate were washed away, 100 μl freshly prepared substrate solution (tetramethyl benzidine, TMB) was added to each well. The reaction was allowed to proceed for 10 minutes and the color development was stopped by the addition of 100 μl / well 1.0 M H₃PO₄. The absorbance at 450 nm and the absorbance at a reference wavelength of 650 nm (A₄5o_/65o) were measured using an ELISA reader. The inhibition efficacy of each test compound is expressed as an inhibition percentage calculated according to the following formula: 1-[(C-A) / (B-A)]. In this formula, A is the basal amount of phosphotyrosine detected in a blank control, B is the amount of phosphotyrosine detected with VEGF-C only, and C is the amount of phosphotyrosine detected with a test compound and VEGF-C.

Among the 55 compounds, 50 compounds (i.e., compounds 1-22, 24-30, 32, 34- 39, 41-50, and 52-55) were tested. Unexpectedly, 46 of the test compounds showed more than 20% inhibition of VEGF receptor 3. Among the 46 compounds, 24 showed more than 50% inhibition, and 5 showed more than 75% inhibition.

Example 57: In vivo assay

Compound 1 was tested for its efficacy in inhibiting tumor growth on murine tumor xenografts. Briefly, VEGF-C overexpressing H928 cells or LLC were trypsinized, washed with PBS and resuspended in PBS. The concentration was adjusted to 3xlO⁶ cells/100 μl in PBS. The cell suspension was then injected subcutaneously into the right abdominal wall of C57BL/6J mice (7-8 week old, one tumor per mice). When the diameter of implanted tumor cells reached 5 mm, compound 1 or vehicle was administered intraperitoneally once daily. The length and width of the tumor was measured every 2-3 days by using a caliper. The tumor volume was then calculated as follows: volume=length x width² x 0.52. Student's t test was used to compare tumor volumes, with P<0.05 being considered significant. After 8 weeks, the mice were sacrificed in a CO₂ chamber and the tumors were collected. Lungs and lymph nodes were removed. For tumor metastasis assay (Quantitative analysis of lung metastatic nodules), the number of lung tumor nodule was counted under a dissecting microscope. Compound 2 was tested by the same procedure. OTHER EMBODIMENTS

All of the features disclosed in this specification may be combined in any combination. Each feature disclosed in this specification may be replaced by an alternative feature serving the same, equivalent, or similar purpose. Thus, unless expressly stated otherwise, each feature disclosed is only an example of a generic series of equivalent or similar features.

From the above description, one skilled in the art can easily ascertain the essential characteristics of the present invention, and without departing from the spirit and scope thereof, can make various changes and modifications of the invention to adapt it to various usages and conditions. Thus, other embodiments are also within the scope of the following claims.

Claims

WHAT IS CLAIMED IS:

1. A compound of formula (I):

wherein each ^_2Z independently is a double bond or single bond; each of Xi, X₂, X₃, X₄, X₅, X₆, X7, independently, is C or N, provided that at least two Of X₁, X₂, and X₃ are N; and that when X₁ is N, R₁ is deleted, when X₃ is N, R₃ is deleted, when X₄ is N, R₄ is deleted, when X₅ is N, R₅ is deleted, when X₆ is N, R₆ is deleted, and when X₇ is N, R₇ is deleted; each of X₈ and X₉, independently, is C or N⁺; and each of Ri, R₂, R₃, R₄, R₅, R₆, and R₇, independently, is H, C₁-CiO alkyl, C₂- Cio alkenyl, C₂-CiO alkynyl, C₃-C₂O cycloalkyl, C₃-C₂O cycloalkenyl, C1-C20 heterocycloalkyl, C₁-C_2O heterocycloalkenyl, aryl, heteroaryl, halo, CN, NO₂, OR₃, COOR_a, OC(O)R_a, C(O)R₃, C(0)NR_aR_b, C(0)N(R_a)N(R_b)C(0)R_c, NR_aR_b, N(R_c)SO₂NR_aR_b, SO₂NR₃R_b, or SR₃, in which each of R₃, R_b, and R_c, independently, is H, Ci-Cio alkyl, C₃-C₂₀ cycloalkyl, Ci-C_2O heterocycloalkyl, aryl, or heteroaryl, or R₃ and R_b together with the nitrogen atom to which they are attached form a C₁-C_2O heterocycloalkyl or heteroaryl.

2. The compound of claim 1, wherein each _:==z independently is a double bond, each of Xi, X₄, X₅, X₆, X₇, Xg, and Xg, independently, is C, and each of X₂ and X₃, independently, is N.

3. The compound of claim 2, wherein R₂ is C₃-C₂₀ cycloalkyl, C₁-Ci₀ alkyl optionally substituted with aryl or Ci-C_2O heterocycloalkyl, or aryl optionally substituted with C₁-CiO alkyl.

4. The compound of claim 3, wherein R₁ is H or OR_a.

5. The compound of claim 4, wherein each of R₄, R₅, R₆, and R₇, independently, is H, C₁-C₁₀ alkyl, NR_aR_b, COOR₃, C(0)NR_aR_b, C(O)N(R_a)N(R_b)C(O)R_c, or heteroaryl.

6. The compound of claim 5, wherein the compound is one of compounds 1-29, 31-33, 35-47, and 49-55.

7. The compound of claim 1, wherein each _:==z independently is a single bond.

8. The compound of claim 7, wherein the compound is compound 30.

9. A compound of formula (II):

wherein each of X₁, X₂, and X₃, independently, is C or N, provided that at least two of X₁, X₂, and X₃ are N; and that when X₁ is N, R₁ is deleted, when X₂ is N, R₂ is deleted; and each of R₁, R₂, R₃, R₄, R₅, R₆, and R₇, independently, is H, C₁-C_1O alkyl, C₂- C₁₀ alkenyl, C₂-C₁₀ alkynyl, C₃-C₂₀ cycloalkyl, C₃-C₂₀ cycloalkenyl, C₁-C₂₀ heterocycloalkyl, C₁-C₂₀ heterocycloalkenyl, aryl, heteroaryl, halo, CN, NO₂, OR_a, COORa, OC(O)R₃, C(O)R₃, C(0)NR_aR_b, C(O)N(R_a)N(R_b)C(O)R_c, NR_aR_b, N(R_c)SO₂NR_aR_b, SO₂NR_aR_b, or SR₃, in which each of R₃, R_b, and R_c, independently, is H, C₁-C₁₀ alkyl, C₃-C₂₀ cycloalkyl, CrC₂₀ heterocycloalkyl, aryl, or heteroaryl, or R₃ and Rb together with the nitrogen atom to which they are attached form a C₁-C_2O heterocycloalkyl or heteroaryl.

10. The compound of claim 9, wherein X₁ is C, each of X₂ and X₃, independently, is N, and each of R₃, R₄, R₅, R₆, and R₇, independently, is H, Ci-C_1O alkyl, NR_aR_b, COOR₃, C(0)NR_aR_b, C(0)N(R_a)N(R_b)C(0)R_c, or heteroaryl.

11. The compound of claim 10, wherein the compound is one of compounds 34 and 48.

12. A method for treating cancer, comprising administering to a subject in need thereof an effective amount of a compound of formula (I):

wherein each ^^ independently is a double bond or single bond; each of X₁, X₂, X₃, X₄, X₅, X₆, and X₇, independently, is C or N, provided that at least two of X₁, X₂, and X₃ are N; and that when X₁ is N, R₁ is deleted, when X₃ is N, R₃ is deleted, when X₄ is N, R₄ is deleted, when X₅ is N, R₅ is deleted, when X₆ is N, R₆ is deleted, and when X₇ is N, R₇ is deleted; each of X₈ and Xg, independently, is C or N⁺; and each of R₁, R₂, R₃, R₄, R₅, R₆, and R₇, independently, is H, C₁-C₁₀ alkyl, C₂- C₁₀ alkenyl, C₂-C₁₀ alkynyl, C₃-C₂₀ cycloalkyl, C₃-C₂₀ cycloalkenyl, Ci-C₂₀ heterocycloalkyl, Ci-C₂₀ heterocycloalkenyl, aryl, heteroaryl, halo, CN, NO₂, OR_a, COOR_a, OC(O)R₃, C(O)R₃, C(0)NR_aR_b, C(O)N(R₃)N(R_b)C(O)R_c, NR₃R₅, N(R_c)SO₂NR_aR_b, SO₂NR₃R_b, or SR₃, in which each Of R₃, R_b, and R_c, independently, is H, Ci-Ci₀ alkyl, C₃-C₂₀ cycloalkyl, CrC₂₀ heterocycloalkyl, aryl, or heteroaryl, or R₃ and Rb together with the nitrogen atom to which they are attached form a C₁-C_2O heterocycloalkyl or heteroaryl.

13. The method of claim 12, wherein each of X₁, X₄, X₅, X₆, X₇, Xs, and X₉, independently, is C and each of X₂ and X₃, independently, is N.

14. The method of claim 13, wherein R₂ is C₃-C_2O cycloalkyl, C₁-C_1O alkyl optionally substituted with aryl or C₁-C_2O heterocycloalkyl, or aryl optionally substituted with C₁-C_1O alkyl.

15. The method of claim 14, wherein R₁ is H or OR₈.

16. The method of claim 15, wherein each of R₄, R₅, R₆, and R₇, independently, is H, C₁-C₁₀ alkyl, NR_aR_b, COOR₃, C(0)NR_aR_b, C(O)N(R_a)N(R_b)C(O)Rc, or heteroaryl.

17. A method for treating cancer, comprising administering to a subject in need thereof an effective amount of a compound of formula (II):

wherein each of X₁, X₂, and X₃, independently, is C or N, provided that at least two of X₁, X₂, and X₃ are N; and that when X₁ is N, R₁ is deleted, when X₂ is N, R₂ is deleted; and each of R₁, R₂, R₃, R₄, R₅, R₆, and R₇, independently, is H, C₁-C₁₀ alkyl, C₂- C₁₀ alkenyl, C₂-C₁₀ alkynyl, C₃-C₂₀ cycloalkyl, C₃-C₂₀ cycloalkenyl, C₁-C₂₀ heterocycloalkyl, C₁-C_2O heterocycloalkenyl, aryl, heteroaryl, halo, CN, NO₂, OR_a, COOR_a, OC(O)R₃, C(O)R₃, C(0)NR_aR_b, C(0)N(R_a)N(R_b)C(0)R_c, NR_aR_b, N(R_c)SO₂NR_aR_b, SO₂NR₃Rb, or SR₃, in which each of R₃, R_b, and R_c, independently, is H, C₁-C_1O alkyl, C₃-C_2O cycloalkyl, CrC_2O heterocycloalkyl, aryl, or heteroaryl, or R₃ and Rb together with the nitrogen atom to which they are attached form a Ci-C_2O heterocycloalkyl or heteroaryl.

18. The method of claim 17, wherein X₁ is C, each of X₂ and X₃, independently, is N, and each of R₃, R₄, R₅, R₆, and R₇, independently, is H, C₁-Ci_O alkyl, NR_aR_b, COOR₃, C(0)NR_aR_b, C(0)N(R_a)N(R_b)C(0)R_c, or heteroaryl.

19. A pharmaceutical composition, comprising a pharmaceutically acceptable carrier and a compound of formula (I):

wherein each _Z1Zz independently is a double bond or single bond; each of X₁, X₂, X₃, X₄, X₅, X₆, and X₇, independently, is C or N, provided that at least two of X₁, X₂, and X₃ are N; and that when X₁ is N, R₁ is deleted, when X₃ is N, R₃ is deleted, when X₄ is N, R₄ is deleted, when X₅ is N, R₅ is deleted, when X₆ is N, R₆ is deleted, and when X₇ is N, R₇ is deleted; each of X₈ and X₉, independently, is C or N⁺; and each of Ri, R₂, R₃, R₄, R₅, R₆, and R₇, independently, is H, Ci-C₁₀ alkyl, C₂- C₁₀ alkenyl, C₂-Ci₀ alkynyl, C₃-C₂₀ cycloalkyl, C₃-C₂₀ cycloalkenyl, C₁-C₂₀ heterocycloalkyl, C₁-C₂₀ heterocycloalkenyl, aryl, heteroaryl, halo, CN, NO₂, OR₃, COOR₃, OC(O)R₃, C(O)R₃, C(0)NR_aR_b, C(0)N(R₃)N(R_b)C(0)R_c, NR_aR_b, N(R_c)SO₂NR_aR_b, SO₂NR₃R_b, or SR₃, in which each of R₃, R_b, and R_c, independently, is H, Ci-Ci₀ alkyl, C₃-C₂₀ cycloalkyl, Ci-C_2O heterocycloalkyl, aryl, or heteroaryl, or R₃ and R_b together with the nitrogen atom to which they are attached form a Ci-C₂O heterocycloalkyl or heteroaryl.

20. The composition of claim 19, wherein each of X₁, X₄, X₅, X₆, X₇, X₈, and Xg, independently, is C and each of X₂ and X₃, independently, is N.

21. The composition of claim 20, wherein R₂ is C₃-C₂₀ cycloalkyl, C₁-C₁₀ alkyl optionally substituted with aryl or C₁-C_2O heterocycloalkyl, or aryl optionally substituted with C₁-C₁₀ alkyl.

22. The composition of claim 21, wherein R₁ is H or OR₃.

23. The composition of claim 22, wherein each of R₄, R₅, R₆, and R₇, independently, is H, C₁-C₁₀ alkyl, NR_aR_b, COOR₃, C(0)NR_aR_b, C(0)N(R_a)N(R_b)C(0)Rc, or heteroaryl.

24. A pharmaceutical composition, comprising a a pharmaceutically acceptable carrier and a compound of formula (II):

wherein each of X₁, X₂, and X₃, independently, is C or N, provided that at least two of X₁, X₂, and X₃ are N; and that when X₁ is N, R₁ is deleted, when X₂ is N, R₂ is deleted; and each of Ri, R₂, R₃, R₄, R₅, R₆, and R₇, independently, is H, Ci-C₁₀ alkyl, C₂- CiQ alkenyl, C₂-Ci₀ alkynyl, C₃-C₂₀ cycloalkyl, C₃-C₂₀ cycloalkenyl, C₁-C₂₀ heterocycloalkyl, C₁-C₂₀ heterocycloalkenyl, aryl, heteroaryl, halo, CN, NO₂, OR_a, COOR_a, OC(O)R₃, C(O)R₃, C(0)NR_aR_b, C(O)N(R₃)N(R_b)C(O)Rc, NR_aR_b, N(R_c)SO₂NR₃R_b, SO₂NR₃R_b, or SR₃, in which each of R₃, R_b, and R_c, independently, is H, Ci-C₁₀ alkyl, C₃-C₂₀ cycloalkyl, C]-C₂₀ heterocycloalkyl, aryl, or heteroaryl, or R_a and R_b together with the nitrogen atom to which they are attached form a C₁-C_2O heterocycloalkyl or heteroaryl.

25. The compound of claim 24, wherein Xi is C, each of X₂ and X₃, independently, is N, and each of R₃, R₄, R₅, R₆, and R₇, independently, is H, C₁-Ci_O alkyl, NR_aR_b, COOR₃, C(0)NR_aR_b, C(0)N(R_a)N(R_b)C(0)R_c, or heteroaryl.