WO2008063116A1 - Indozalyl sulphonamide derivatives useful as glucocorticoid modulators - Google Patents

Indozalyl sulphonamide derivatives useful as glucocorticoid modulators Download PDF

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Publication number
WO2008063116A1
WO2008063116A1 PCT/SE2007/001031 SE2007001031W WO2008063116A1 WO 2008063116 A1 WO2008063116 A1 WO 2008063116A1 SE 2007001031 W SE2007001031 W SE 2007001031W WO 2008063116 A1 WO2008063116 A1 WO 2008063116A1
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WIPO (PCT)
Prior art keywords
alkyl
propan
indazol
oxy
fluorophenyl
Prior art date
Application number
PCT/SE2007/001031
Other languages
French (fr)
Inventor
Markus Berger
Lena BERGSTRÖM
Jan Dahmén
Anders Eriksson
Balint Gabos
Martin Hemmerling
Krister Henriksson
Svetlana Ivanova
Matti Lepistö
Darren Mckerrecher
Stinabritt Nilsson
Hartmut Rehwinkel
Camilla Taflin
Original Assignee
Astrazeneca Ab
Bayer Schering Pharma Aktiengesellschaft
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Publication date
Application filed by Astrazeneca Ab, Bayer Schering Pharma Aktiengesellschaft filed Critical Astrazeneca Ab
Priority to CA002677335A priority Critical patent/CA2677335A1/en
Priority to EP07835228A priority patent/EP2097384B1/en
Priority to ES07835228T priority patent/ES2393009T3/en
Priority to CN2007800436841A priority patent/CN101646654B/en
Priority to JP2009538365A priority patent/JP2010510985A/en
Publication of WO2008063116A1 publication Critical patent/WO2008063116A1/en
Priority to HK10105774.9A priority patent/HK1138852A1/en

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Definitions

  • the present invention relates to novel indazolyl sulphonamide derivatives, to pharmaceutical compositions comprising such derivatives, to processes for preparing such novel derivatives and to the use of such derivatives as medicaments (for example in the treatment of an inflammatory disease state).
  • Sulphonamide derivatives are disclosed as anti-inflammatories in WO 2004/019935 and WO 2004/050631.
  • Pharmaceutically active sulphonamides are also disclosed in Arch. Pharm. (1980) 313 166-173, J.Med. Chem. (2003) 46 64-73, J. Med. Chem (1997) 40 996- 1004, EP 0031954, EP 1190710 (WO 200124786), US 5861401, US 4948809, US3992441 and WO 99/33786.
  • non-steroidal compounds interact with the glucocorticoid receptor (GR) and, as a result of this interaction, produce a suppression of inflammation (see, for example, US6323199).
  • GR glucocorticoid receptor
  • Such compounds can show a clear dissociation between anti-inflammatory and metabolic actions making them superior to earlier reported steroidal and non-steroidal glucocorticoids.
  • the present invention provides further non-steroidal compounds as modulators (for example agonists, antagonists, partial agonists or partial antagonists) of the glucocorticoid receptor.
  • the present invention provides a compound of formula (I):
  • A is Ci-ioalkyl, C5 -10 aryl, C 5 . 10 heteroaryl, Cs-ioheteroarylCi-ealkyl, C 5- 10 arylCi_ 6 alkoxy, Ci-iohaloalkyl, C 3-7 cycloalkyl, C 3 - 7 cycloalkylCi_ 4 alkyl, Ci- 6 alkyl0C(0)Ci. 6 alkyl, C 1-6 alkylC(O)OC 1-6 alkyl , C 5 . 10 aryloxyC MO alkyl or NR 5 R 6 C 0 .
  • R 1 and R la are independently selected from hydrogen, Ci- 4 alkyl, Ci -4 haloalkyl, Ci- ⁇ lydroxyalkyl and Ci ⁇ alkylOCi ⁇ alkyl;
  • R 2 is hydrogen or C 1-4 alkyl;
  • R 3 is C 3-7 cycloalkyl (optionally subtituted by halogen or C 1-6 alkyl), Cs-ioarylCooalkyl, C 5 . ]oarylOCo- 3 alkyl, Ci_ 6 alkyl, Ci_ 6 alkenyl or Ci- 6 alkynyl which are optionally substituted by one or more B;
  • B is C 0-3 hydroxyalkyl, C 1-4 alkyl, C 1-4 alkoxy, C 0 _ 4 alkylthioC 0 ⁇ alkyl, C 3 - 6 cycloalkylC 0- 4 thioalkyl, C 0-3 alkylS(0) n Co -4 alkyl, Q- ⁇ haloalkyl, C 1-4 haloalkoxy, halogen, nitro, cyano, C M alkylOCi.
  • R 4 is hydrogen, hydroxy, halogen, C 1-4 alkyl or C 1-4 haloalkyl
  • W is hydrogen, C 3-7 cycloalkyl, phenyl, thienyl, isoxazolyl, pyrazolyl, pyridinyl or pyrimidinyl all optionally substituted with one or more substituents selected from halogen, Co- 3 hydroxyalkyl, d.
  • X is CH 2 , O, S, S(O), S(O) 2 or NH;
  • Y is hydrogen, halogen, Ci -6 alkyl, nitro, cyano, hydroxy, R 5 C(O), R 5 OC(O), R 5 C(O)O, S(O) n Ci -4 alkyl, R 5 R 6 NS(O) n , benzyloxy, imidazolyl, Ci -4 alkylNHC(O), NR 5 R 6 C(O), C M alkylC(O)NH or NR 5 R 6 ;
  • R 5 and R 6 are independently selected from hydrogen, Ci -4 alkyl and C 3 -- 7 cycloalkyl, or R 5 and R 6 form together a group -(O)C 5 .i 0 arylC(O)-; and n is 1 or 2, or a pharmaceutically acceptable salt thereof.
  • One embodiment relates to compound of formula (I), wherein: A is Ci-ioalkyl, Cs-ioaryl, Cs-ioarylCi.galkyl, C 5 _ioheteroaryl ; Cs.ioheteroarylCi- ⁇ alkyl, C 5- ioarylCi. 6 alkoxy, Ci-iohaloalkyl, C 3-7 cycloalkyl, C 3-7 cycloalkylCi_ 4 alkyl, C 1 .
  • R 1 and R la are independently selected from hydrogen and Ci -4 alkyl;
  • R 2 is hydrogen
  • R 3 is C 5- ioarylCo -3 alkyl, or C 5- ioheteroary ICo -3 alkyl which are optionally substituted by one or more B;
  • B is Co ⁇ hydroxyalkyl, C 1-4 alkyl, C 1-4 alkoxy, Ci- ⁇ haloalkyl, C 1-4 haloalkoxy, halogen or NR 5 R 6 C 0-4 alkyl;
  • R 4 is hydrogen
  • W is C 3-7 cycloalkyl, C 1-4 alkyl, phenyl or pyridinyl all optionally substituted with one or more substituents selected from halogen;
  • X is O, S or S(O) 2 ;
  • Y is hydrogen or halogen;
  • R 5 and R 6 are independently selected from hydrogen and C 1-4 alkyl, or R 5 and R 6 form together a group -(O)C 5-10 arylC(O)-, or a pharmaceutically acceptable salt thereof.
  • A is Ci.ioalkyl, C 1-10 haloalkyl, C 3-7 cycloalkyl, C 3-7 cycloalkylC 1-4 alkyl, ⁇ alkyl, Ci. 6 alkylC(O)OCi- 6 alkyl or NR 5 R 6 C 0- 6alkyl optionally substituted with one or more substituents selected from B.
  • R 1 and R la are independently selected from hydrogen and Ci -4 alkyl;
  • R 2 is hydrogen;
  • R 3 is Cs-ioarylCo- 3 alkyl, which are optionally substituted by one or more B;
  • B is Co- 3 hydroxyalkyl, Ci ⁇ alkyl, Ci -4 alkoxy, Ci. ⁇ haloalkyl, Ci -4 haloalkoxy, halogen or
  • W is C 3-7 cycloalkyl, phenyl or pyridinyl all optionally substituted with one or more substituents selected from halogen;
  • X is O, S or S(O) 2 ;
  • Y is hydrogen or halogen;
  • R 5 and R 6 are independently selected from hydrogen and Cj -4 alkyl, or R 5 and R 6 form together a group -(O)C 5 . 10 arylC(O)-, or a pharmaceutically acceptable salt thereof.
  • A is C 5 ,ioaryl, whereby the aryl is optionally substituted with one or more substituents selected from B;
  • R 1 and R la are independently selected from hydrogen and C 1-4 alkyl;
  • R 2 is hydrogen
  • R 3 is C 5 -i O arylCo. 3 alkyl, C 5- i 0 arylOC 0-3 alkyl or which are optionally substituted by one or more B;
  • B is C 0 _ 3 hydroxyalkyl, Q ⁇ alkyl, C 1-4 alkoxy, Ci.ehaloalkyl, Ci -4 haloalkoxy, halogen or NR 5 R 6 Co -4 alkyl;
  • R 4 is hydrogen
  • W is C 3 . 7 cycloalkyl, C ⁇ alkyl, phenyl or pyridinyl all optionally substituted with one or more substituents selected from halogen;
  • X is O, S or S(O) 2 ;
  • Y is hydrogen or halogen;
  • R 5 and R 6 are independently selected from hydrogen and C 1-4 alkyl, or R 5 and R 6 form together a group -(O)C5 -10 arylC(O)-, or a pharmaceutically acceptable salt thereof.
  • a further embodiment relates to compound of formula (I), wherein:
  • A is C 5- ioheteroaryl or Cs-ioheteroarylCi- ⁇ alkyl optionally substituted with one or more substituents selected from B;
  • R 1 and R la are independently selected from hydrogen and C 1-4 alkyl
  • R 2 is hydrogen;
  • R 3 is C 5 .ioarylC o-3 alkyl, C 5-1 oarylOCo -3 alkyl or Cs.ioheteroarylCo-salkyl which are optionally substituted by one or more B;
  • B is C 0 - 3 hydroxyalkyl, C 1-4 alkyl, Ci -4 alkoxy, Q- ⁇ haloalkyl, Ci -4 haloalkoxy, halogen or NR 5 R 6 C 0 _ 4 alkyl;
  • R 4 is hydrogen;
  • W is C 3-7 cycloalkyl, Ci. 4 alkyl, phenyl or pyridinyl all optionally substituted with one or more substituents selected from halogen;
  • X is O, S or S(O) 2 ;
  • Y is hydrogen or halogen;
  • R 5 and R 6 are independently selected from hydrogen and C 1-4 alkyl, or R 5 and R 6 form together a group -(O)C5 -10 arylC(O)-, or a pharmaceutically acceptable salt thereof.
  • A is Ci-i O alkyl, C 5-1O aTyI, Cs- ⁇ aryld-ealkyl, Cs.ioheteroaryl, Cs ⁇ oheteroarylC ⁇ alkyl, C 5- ioaryld- ⁇ alkoxy, C ⁇ ohaloalkyl, C 3-7 cycloalkyl, C 3 - 7 cycloalkylC 1-4 alkyl, C 1- 6 alkyl0C(0)Ci.
  • R 1 and R la are independently selected from hydrogen, C 1-4 alkyl, C 1-4 haloalkyl, C 1-
  • R 2 is hydrogen or R 3 is C 3-7 cycloalkyl (optionally subtituted by halogen or C 1-6 alkyl), Cs -lo arylCo -3 alkyl, C 5- ioarylOCo- 3 alkyl, C 5- ioheteroarylCo -3 alkyl, or C]. 6 alkynyl which are optionally substituted by one or more B;
  • B is Co -3 hydroxyalkyl, C 1-4 alkyl, C 1-4 alkoxy, Co ⁇ alkylthioCo ⁇ alkyl, C 3-6 cycloalkylCo-
  • R 4 is hydrogen, hydroxy, halogen, C 1-4 alkyl or Ci -4 haloalkyl;
  • W is C 3-7 cycloalkyl, Ci -4 alkyl, phenyl, thienyl, isoxazolyl, pyrazolyl, pyridinyl or pyrimidinyl all optionally substituted with one or more substituents selected from halogen, Co- 3 hydroxyalkyl, Q ⁇ alkyl, Ci- 4 alkoxy, Co- 4 alkylthioCo- 4 alkyl, C 3 .
  • Y is hydrogen, halogen, C 1-6 alkyl, C 1-4 alkoxy, Ci -4 thioalkyl, Ci -4 haloalkyl, Ci -4 alkoxyhalo, nitro, cyano, hydroxy, R 5 C(O), R 5 OC(O), R 5 C(O)O, S(O) n C 1-4 alkyl, R 5 R 6 NS(O) n , I 0 benzyloxy, imidazolyl, Ci -4 alkylNHC(O), NR 5 R 6 C(O), or NR 5 R 6 ;
  • R 5 and R 6 are independently selected from hydrogen, Ci -4 alkyl and C 3-7 cycloalkyl, or R 5 and R 6 form together a group -(0)C 5- ioarylC(0)-; and n is 1 or 2, or a pharmaceutically acceptable salt thereof.
  • A is C ⁇ ioalkyl, C 5- i 0 aryl, C 5- i 0 arylCi -6 alkoxy, Ci-iohaloalkyl, C3 -7 cycloalkyl, C 3-7 CyClOaIlCyIC 1 ⁇ alkyl, Q- 6 alkyl0C(0)C 1-6 alkyl, C 1-6 alkylC(O)OC 1-6 alkyl, C 5 -ioaryloxyCi -10 alkyl or NR 5 R 6 Co -6 alkylo whereby the aryl is optionally substituted with one or more substituents selected from B.
  • A is C 3- 6cycloalkyl. In another embodiment A is cyclopropyl or cyclopentyl.
  • A is C 3- 6cycloalkylCi -2 alkyl. In yet another embodiment A is cyclopentyl-methyl. 5 In a further embodiment A is C ⁇ ioalkyl. In one embodiment A is methyl, ethyl, n-propyl, i- propyl, n-butyl, i-butyl, s-butyl, t-butyl, n-pentyl, i-pentyl, neo-pentyl, n-hexyl or i-hexyl.
  • A is methyl. In another embodiment A is n-propyl. In yet another embodiment A is n-pentyl or s-pentyl. In yet a further embodiment A is s-butyl or n-butyl.
  • A is n-hexyl. 0 In one embodiment A is C ⁇ haloalkyl, In another embodiment A is fluoromethyl, difluoromethyl, trifluoromethyl, fluoroethyl, difluoroethyl, trifluoroethyl or trifluoropropyl. In another embodiment A is trifluoromethyl. In yet another embodiment A is trifluoromethyl, trifluoroethyl or trifluoropropyl.
  • A is Cs-ioheteroarylCi- ⁇ alkyl. In one embodiment A is dioxoisoindolyl-ethyl.
  • A is Cs- ⁇ heteroaryl optionally substituted with B.
  • A is imidazolyl or isooxazolyl optionally substituted with B.
  • A is imidazolyl or isooxazolyl substituted with one or two methyl.
  • A is optionally substituted with C] -4 alkoxy.
  • A is phenyl-ethoxy substituted with methoxy.
  • A is C 1-4 alkylOC(0)C M alkyl. In one embodiment A is Q-
  • A is propanoate.
  • A is Cs.ioheteroaryl. In a further embodiment A is pyridinyl.
  • R 1 and R la are independently selected from hydrogen and C 1-4 alkyl.
  • R 1 is hydrogen.
  • R 1 is methyl.
  • R la is hydrogen.
  • R 2 is hydrogen.
  • R 3 is Cs.ioarylCo-salkyl, C 5- 10 arylOCo- 3 alkyl or Cs-ioheteroarylCooalkyl which are optionally substituted by one or more B.
  • R 3 is Cs-earyl.
  • R 3 is phenyl.
  • R 3 is phenyl substituted with B.
  • R 3 is phenyl substituted with halogen or R 5 S(O) 2 , wherein R 5 is d ⁇ alkyl.
  • B is fluoro.
  • B is methyl-S(O) 2 .
  • R 3 is Cs -6 heteroaryl. In one embodiment R 3 is pyridinyl substituted with B. In yet a further embodiment R 3 is pyridinyl substituted with C 1-4 alkoxy. In one embodiment R 3 is pyridinyl substituted with methoxy. In another embodiment R 3 together with B form a dihydrobenzodioxinyl group as in example 43.
  • R 1 is C 1 .4a.kyl
  • R la is hydrogen
  • R 2 is hydrogen
  • R 3 is Cs-ioaryl, whereb »yy aryl is optionally substituted by one or more B.
  • B is halogen or R 5 S(O) 2 .
  • W is C 3-7 CyClOaIlCyI, phenyl or pyridinyl all optionally substituted with one or more substituents selected from halogen.
  • W is C 3-6 CyClOaIlCyI, Ci -4 alkyl, phenyl or pyridinyl optionally substituted with one substituent selected from halogen.
  • W is phenyl.
  • W is phenyl substituted with halogen.
  • W is phenyl substituted with fluoro.
  • W is cyclopentyl. In another embodiment W is iso-propyl. In yet another embodiment W is pyridinyl.
  • One embodiment relates to compounds of formula I wherein X is O. In another embodiment X is S. In a further embodiment X is S(O) 2.
  • R 1 is Q ⁇ alkyl
  • R la is hydrogen
  • R is hydrogen
  • X is O
  • R is Cs-ioaryl
  • B is halogen or R 5 S(O) 2 .
  • One embodiment relates to compounds of formula I wherein Y is hydrogen. In another embodiment Y is halogen. In a further embodiment Y is chloro.
  • R 5 and R 6 are independently selected from hydrogen and Ci -4 alkyl.
  • R 5 and R 6 form together a group -(O)C 5-10 arylC(O)-.
  • Another embodiment relates to compounds of formula (IA)
  • One embodiment relates to compounds of formula (I) wherein the group:
  • alkyl includes both straight and branched chain alkyl groups and may be, but are not limited to methyl, ethyl, n-propyl, i- propyl, n-butyl, i-butyl, s-butyl, t-butyl, n-pentyl, i-pentyl, neo-pentyl, n-hexyl or i-hexyl.
  • the term having 1 to 4 carbon atoms and may be but are not limited to methyl, ethyl, n-propyl, i-propyl or tert-buty[.
  • Co refers to a situtation where no carbon atom is present.
  • alkoxy refers to radicals of the general formula -O-R, wherein R is selected from a hydrocarbon radical.
  • alkoxy may include, but is not limited to methoxy, ethoxy, propoxy, isopropoxy, butoxy, t-butoxy, isobutoxy, cyclopropylmethoxy, allyloxy or propargyloxy.
  • cycloalkyl refers to an optionally substituted, partially or completely saturated monocyclic, bicyclic or bridged hydrocarbon ring system.
  • the term "Ci- ⁇ cycloalkyl” may be, but is not limited to cyclopropyl, cyclobutyl, cyclopentyl or cyclohexyl.
  • haloalkyl means an alkyl group as defined above, which is substituted with halogen as defined above.
  • C 1 - ⁇ haloalkyl may include, but is not limited to fluoromethyl, difluoromethyl, o trifluoromethyl, fluoroethyl, difluoroethyl or bromopropyl.
  • C 1-3 haloalkylO may include, but is not limited to fluoromethoxy, difluoromethoxy, trifluoromethoxy, fluoroethoxy or difluoroethoxy.
  • C 5-1 oaryl refers to an aromatics group such as for example, phenyl or naphthyl.
  • Cs.ioheteroaryl refers to a mono- or bicyclic aromatic or partially aromatic ring containing one or more heteroatoms independently selected from nitrogen, oxygen, sulphur.
  • heteroaryls are oxazolyl, furyl, thienyl, pyrrolyl, pyrazolyl, imidazolyl, pyridinyl, pyrimidinyl, indolyl,o indazolyl, benzfuryl or benzthienyl.
  • Heteroaryl may also be dihydrobenzodioxinyl, quinolinyl or isoquinolinyl.
  • Cs.ioary 1C 1-4 alkyl is for example benzyl.
  • Cs- t oarylCi ⁇ alkoxy is, for example a methoxy 5 substituted with a phenyl.
  • Ci -4 alkoxyC 5 . 1 oaryl is, for example, a phenyl substituted with methoxy.
  • the compounds of the invention are selected from N-[(l R,2S)- 1 -[ 1 -(4-fluorophenyl)indazol-5-yl]oxy- 1 -phenyl-propan-2- yljcyclopropanesulfonamide,
  • the present invention provides the individual compound: N-[(l R,2S)- 1 -[ 1 -(4-fluorophenyl)indazol-5-yl]oxy- 1 -phenyl-propan-2- yljcyclopropanesulfonamide or a pharmaceutically acceptable salt thereof.
  • the compounds of formula (I) and pharmaceutically acceptable salts thereof may exist in solvated, for example hydrated or unsolvated forms, as well as cocrystalline forms and the present invention encompasses all such forms.
  • Compounds of formula (I) above may be converted to a pharmaceutically acceptable salt thereof, preferably an acid addition salt such as a hydrochloride, hydrobromide, phosphate, sulfphate, acetate, ascorbate, benzoate, fumarate, hemifumarate, furoate, succinate, maleate, tartrate, citrate, oxalate, xinafoate, methanesulphonate,/?-toluenesulphonate, benzenesulphonate, ethanesulphonate, 2-naphthalenesulfonate, mesytilenesulfonate, nitric acid, 1,5-naphthalene-disulphonate, p-xylenesulphonate, aspartate or glutamate.
  • an acid addition salt such as a hydrochloride, hydrobromide, phosphate, sulfphate, acetate, ascorbate, benzoate, fuma
  • They may also include basic addition salts such as an alkali metal salt for example sodium or potassium salts, an alkaline earth metal salt for example calcium or magnesium salts, a transition metal salt such as a zinc salt, an organic amine salt for example a salt of triethylamine, diethylamine, morpholine, iV-methylpiperidine, TV-ethylpiperidine, piperazine, procaine, dibenzylamine, N,N-dibenzylethylamine, choline or 2-aminoethanol or amino acids for example lysine or arginine.
  • basic addition salts such as an alkali metal salt for example sodium or potassium salts, an alkaline earth metal salt for example calcium or magnesium salts, a transition metal salt such as a zinc salt, an organic amine salt for example a salt of triethylamine, diethylamine, morpholine, iV-methylpiperidine, TV-ethylpiperidine, piperazine, proca
  • Compounds of formula (I) may include an asymmetric centre and be chiral in nature. Where the compound is chiral, it may be in the form of a single stereoisomer, such as a enantiomer, or it may be in the form of mixtures of these stereoisomers in any proportions, including racemic mixtures. Therefore, all enantiomers, diastereomers, racemates and mixtures thereof are included within the scope of the invention.
  • the various optical isomers may be isolated by separation of a racemic mixture of the compounds using conventional techniques, for example, fractional crystallisation, or HPLC. Alternatively the optical isomers may be obtained by asymmetric synthesis, or by synthesis from optically active starting materials.
  • the compounds of formula (I) can be prepared using or adapting methods disclosed in the art, or by using or adapting the method disclosed in the Example below.
  • Starting materials for the preparative methods are either commercially available or can be prepared by using or adapting literature methods.
  • One embodiment the present invention relates to a process for the preparation of compounds of formula (I) by coupling a compound of formula (II):
  • L 1 is a leaving group (such as halogen (for example chloro) or mesylate or tosylate), in a suitable solvent (such as pyridine, THF or DMF), in the presence of a suitable base (such as a tri(Ci -6 alkyl)amine, for example diisopropylethylamine, or pyridine) and at a suitable temperature (such as -10 to 50 0 C).
  • a suitable solvent such as pyridine, THF or DMF
  • a suitable base such as a tri(Ci -6 alkyl)amine, for example diisopropylethylamine, or pyridine
  • One embodiment the present invention relates to a process for the preparation of compounds of formula (II), wherein X is O, S or NH, (a) by coupling a compound of formula (IV) wherein R 4 , W and Y are defined as in compounds of formula (I) and L 2 is a leaving group (such as halogen or triflate) with a compound of formula (V)
  • R 1 , R la and R 2 are defined as in compounds of formula (I) and G corresponds to R 3 or a protected precurser to R 3 and whereby the reaction can be performed in a suitable solvent (such as an aromatic solvent, for example toluene) or a polar, aprotic solvent, such as DMF or butyronitril, in the presence of a suitable base (such as a alkali metal alkoxide (for example sodium tert-butoxide) or, cesium carbonate, preferable mediated by a suitable metal catalyst such as Copper(I) iodide at a suitable temperature (for example in the range 80° to 12O 0 C), or, (b) reacting a compound of formula (VTI)
  • a suitable solvent such as an aromatic solvent, for example toluene
  • a polar, aprotic solvent such as DMF or butyronitril
  • a suitable base such as a alkali metal alkoxide (for example sodium tert-butoxide
  • R 1 , R 2 , R 4 , X, W and Y are defined as in compounds of formula (I)
  • G corresponds to R 3 or a protected precurser to R 3 and L 3 is a leaving group (such as halogen, mesylate or tosylate), and whereby the reaction can be performed in a suitable solvent (such as DCM, DMF or acetonitrile), in the presence of a suitable base (such as an alkali metal carbonate, for example cesium carbonate or potassium carbonate) at a suitable temperature (for example in the range -10 to 5O 0 C), followed by a subsequent reductive amination step using or adopting literature methods, or,
  • R 1 , R la , R 2 and R 3 are defined as in compounds of formula (I) and PG is a suitable protecting group such as BOC, Ms, Ns, Ts or related carbonyl-or sulfonyl residues, and whereby the reaction can be performed in a suitable solvent such as DCM or toluene in the presence of a suitable base such as NaH or KOtBu, followed by a deprotection step using or adopting literature methods.
  • PG is a suitable protecting group such as BOC, Ms, Ns, Ts or related carbonyl-or sulfonyl residues
  • R 1 , R 2 and G are defined as in compounds of formula (V).
  • R 1 , R la are defined as in compounds of formula (I) and G corresponds to R 3 or a protected precurser to R 3 and L is a leaving group (such as alkoxy, methoxy(methyl)amino).
  • M is a metal such as Li or Mg-halide.
  • the addition of the nucleophile may be performed in a suitable aprotic solvent such as THF at moderate temperature between -10 and 5O 0 C. The following reduction and deprotection steps might be carried out by using or adopting literature methods.
  • compounds of formula (X) may be prepared by a reaction of a nuceophile G- M with an aldehyde of formula (XIII) and a subsequent deprotection.
  • R 1 , R la are defined as in compounds of formula (I) and are defined as in compounds of formula (I)
  • G corresponds to R 3 or a protected precurser to R 3 and PG is a protecting group or hydrogen.
  • M is a metal such as an alkali metal (e.g. Li) or Mg-halide.
  • the reaction may be performed by following disclosed protocols for addition of carbanions to aldehydes.
  • R 1 and R la are defined as in compounds of formula (I)
  • G corresponds to R 3 or a protected precurser to R 3
  • PG is a protecting group or hydrogen. Both steps may be carried out by following or adopting literature methods.
  • the invention relates to processes for the preparation of the compounds of formula (I) as well as the intermediates.
  • a compound of formula (I), or a pharmaceutically acceptable salt thereof can be used as a medicament for the treatment or prophylaxis of one or more of the following pathologic conditions (disease states) in a mammal (such as a human):
  • Lung diseases which coincide with inflammatory, allergic and/or proliferative processes: 5 • chronically obstructive lung diseases of any origin, mainly bronchial asthma
  • collagen diseases of other origins for example systemic lupus erythematodes, sclerodermia, polymyositis, dermatomyositis, polyarteritis nodosa, temporalo arteritis
  • eczema • itching (for example of allergic origins) »all forms of eczema, such as, for example, atopic eczema or seborrheal eczema
  • liver diseases which coincide with inflammatory, allergic and/or proliferative processes: • acute liver cell decomposition
  • Gastrointestinal diseases which coincide with inflammatory, allergic and/or proliferative processes:
  • cerebral edema mainly tumor-induced cerebral edema • multiple sclerosis
  • lymphogranulomatoses • lymphosarcoma
  • the compounds of formula (I) can also be used to treat disorders such as: Conies Syndrome, primary and secondary hyperaldosteronism, increased sodium retention, increased magnesium and potassium excretion (diuresis), increased water retention, hypertension (isolated systolic and combined systolic/diastolic), arrhythmias, myocardial fibrosis, myocardial infarction, Bartter's Syndrome, disorders associated with excess catecholamine levels, diastolic and systolic congestive heart failure (CHF), peripheral vascular disease, diabetic nephropathy, cirrhosis with edema and ascites, oesophageal varicies, Addison's Disease, muscle weakness, increased melanin pigmentation of the skin, weight loss, hypotension, hypoglycemia, Cushing's Syndrome, obesity, hypertension, glucose intolerance, hyperglycemia, diabetes mellitus, osteoporosis, poly
  • CHF congestive heart failure
  • 'congestive heart disease refers to a disease state of the cardiovascular system whereby the heart is unable to efficiently pump an adequate volume of blood to meet the requirements of the body's tissues and organ systems.
  • CHF is characterized by left ventricular failure (systolic dysfunction) and fluid accumulation in the lungs, with the underlying cause being attributed to one or more heart or cardiovascular disease states including coronary artery disease, myocardial infarction, hypertension, diabetes, valvular heart disease, and cardiomyopathy.
  • diastolic congestive heart failure refers to a state of CHF characterized by impairment in the ability of the heart to properly relax and fill with blood.
  • systolic congestive heart failure refers to a state of CHF characterized by impairment in the ability of the heart to properly contract and eject blood.
  • physiological disorders may present as a “chronic” condition, or an “acute” episode.
  • chronic means a condition of slow progress and long continuance.
  • a chronic condition is treated when it is diagnosed and treatment continued throughout the course of the disease.
  • acute means an exacerbated event or attack, of short course, followed by a period of remission.
  • the treatment of physiological disorders contemplates both acute events and chronic conditions. In an acute event, compound is administered at the onset of symptoms and discontinued when the symptoms disappear.
  • the present invention provides the use of a compound of formula (I), or a pharmaceutically acceptable salt thereof, for use in therapy (such as a therapy described above).
  • the present invention provides the use of a compound of formula (I), or a pharmaceutically acceptable salt thereof, in the manufacture of a medicament for use in the treatment of a glucocorticoid receptor mediated disease state (such as a disease state described above).
  • the invention provides the use of a compound of formula (I), or a pharmaceutically acceptable salt thereof, in the manufacture of a medicament for use in the treatment of an inflammatory (such as an arthritic) condition.
  • the invention provides the use of a compound of formula (I), or a pharmaceutically acceptable salt thereof, in the manufacture of a medicament for use in the treatment of an asthmatic condition.
  • the invention provides the use of a compound of formula (I), or a pharmaceutically acceptable salt thereof, in the manufacture of a medicament for use in the treatment of COPD.
  • the present invention further provides a method of treating a glucocorticoid receptor mediated disease state, an inflamatory condition, an asthmatic condition and/or COPD, in a mammal (such as man), which comprises administering to a mammal in need of such treatment an effective amount of a compound of formula (I), or a pharmaceutically acceptable salt thereof.
  • the term “therapy” also includes “prophylaxis” unless there are specific indications to the contrary.
  • the terms “therapeutic” and “therapeutically” should be construed accordingly.
  • inhibitor and “antagonist” mean a compound that by any means, partly or completely, blocks the transduction pathway leading to the production of a response by the agonist.
  • 'agonist mean a compound that by any means, partly or completely, provokes the transduction pathway leading to the production of a response.
  • disorder means any condition and disease associated with glucocorticoid receptor activity.
  • the active ingredient is normally formulated in accordance with standard pharmaceutical practice as a pharmaceutical composition. Therefore in another aspect the present invention provides a pharmaceutical composition comprising a compound of formula (I), or a pharmaceutically acceptable salt thereof, (active ingredient) and a pharmaceutically acceptable adjuvant, diluent or carrier. In a further aspect the present invention provides a process for the preparation of said composition comprising mixing the active ingredient with a pharmaceutically acceptable adjuvant, diluent or carrier.
  • the pharmaceutical composition can comprise from 0.05 to 99 %w (per cent by weight), for example from 0.05 to 80 %w, such as from 0.10 to 70 %w (for example from 0.10 to 50 %w), of active ingredient, all percentages by weight being based on total composition.
  • a pharmaceutical composition of the present invention can be administered in a standard manner for the disease condition that it is desired to treat, for example by topical (such as to the lung and/or airways or to the skin), oral, rectal or parenteral administration.
  • a the compound of formula (I), or a pharmaceutically acceptable salt thereof may be formulated into the form of, for example, an aerosol, a powder (for example dry or dispersible), a tablet, a capsule, a syrup, a granule, an aqueous or oily solution or suspension, an (lipid) emulsion, a suppository, an ointment, a cream, drops, or a sterile injectable aqueous or oily solution or suspension.
  • a suitable pharmaceutical composition of this invention is one suitable for oral administration in unit dosage form, for example a tablet or capsule containing between 0.1 mg and 1 g of active ingredient.
  • composition of the invention is one suitable for intravenous, subcutaneous, intraarticular or intramuscular injection.
  • Buffers such as polyethylene glycol, polypropylene glycol, glycerol or ethanol or complexing agents such as hydroxy-propyl ⁇ - cyclodextrin may be used to aid formulation.
  • Tablets may be enteric coated by conventional means, for example to provide a coating of cellulose acetate phthalate.
  • the invention further relates to combination therapies or compositions wherein a GR agonist of formula (I), or a pharmaceutically acceptable salt thereof, or a pharmaceutical composition comprising a GR agonist of formula (I), or a pharmaceutically acceptable salt thereof, is administered concurrently (possibly in the same composition) or sequentially with one or more agents for the treatment of any of the above disease states.
  • a GR agonist of the invention can be combined with one or more agents for the treatment of such a condition.
  • the one or more agents is selected from the list comprising:
  • a PDE4 inhibitor including an inhibitor of the isoform PDE4D
  • adrenoceptor agonist such as metaproterenol, isoproterenol, isoprenaline, albuterol, salbutamol, formoterol, salmeterol, terbutaline, orciprenaline, bitolterol mesylate, pirbuterol or indacaterol
  • a muscarinic receptor antagonist for example a Ml, M2 or M3 antagonist, such as a selective M3 antagonist
  • a steroid such as budesonide
  • a modulator of chemokine receptor function such as a CCRl receptor antagonist
  • the GR agonist of formula (I), or a pharmaceutically acceptable salt thereof can be administered by inhalation or by the oral route and this is in combination with a xanthine (such as aminophylline or theophylline) which can be administered by inhalation or by the oral route.
  • a xanthine such as aminophylline or theophylline
  • the GR agonist of formulao (I) and xanthine may be administered together. They may be administered sequencially. Or they may be administered separately.
  • Method A Instrument Agilent 1100; Column: Kromasil C18 100 x 3 mm, 5 ⁇ particle size, Solvent A: 0.1 %TF A/water, Solvent B: 0.08%TFA/acetonitrile Flow: 1 mL/min, o Gradient 10-100%/B 20 min, 100% B 1 min. Absorption was measured at 220, 254 and 280 nm.
  • the subtitle compound was prepared essentially by the metod described by Job &
  • the acidic waterphase was made basic using KOH (aq) solution, the formed slurry was extracted with ether (3x150 mL). The organic phase was dried (MgSO 4 ) filtered and evaporated. The crude product was further purified by HPLC. Yield 60mg (60%).
  • racemic subtitle compound was prepared as described in Exampel Ia o Starting from racemic (li?5',2iS7?)-2-amino-l-[4-(methyltb.io)plienyl]propan-l-ol (49 mg 0.25 mmol), l-(Fluorophenyl)-5-iodoindazole (27b-erythro, 100 mg, 0.3 mmol), CuI (5 mg, 0.03 mmol), Cs 2 CO 3 (163 mg, 0.5 mmol) in butyronitrile (0.5 mL) at +125°C over night.
  • the acidic waterphase was made basic using KOH (aq) solution, the formed slurry was extracted with ether (3x150 mL). The organic phase was dried (MgSO 4 ) filtered and evaporated. The crude product was further purified and separated into its two racemic diastereomeric pairs by HPLC.
  • the racemic subtitle compound was prepared as described in Exampel 1.
  • the subtitle compound was prepared as described in Exampel 1. (lR,2S)-2-amino-l-[4-(methylthio)phenyl]propan-l-ol (595mg, 3 mmol), 1- (Fluorophenyl)-5-iodoindazole (913 mg, 2.7 mmol), CuI (28 mg, 0.15 mmol), Cs 2 CO 3 (1.95 g, 6 mmol) in butyronitrile (5 mL) and Toluene (2mL) at +125°C for 6 hours. After work up and purification by HPLC the subtitle compound was isolated as the hydrochloride salt by addition of 6-7 N HCl / 2-Propanol solution and repeated evaporations from MeCN to get the subtitle compound as a beige solid. Yield 300 mg
  • the subtitle compound was prepared following the procedure described by Jingjun Yin et. al. J. Org. Chem. 2006, 71, 840-843.
  • the title compound was prepared from (li ⁇ -l-Kl-isopropyl-lH-indazol-S-ytyoxy]-!- phenylpropan-2-amine (30b, 31 mg, 100 ⁇ mol) and cyclopropanelsulfonyl chloride (42 mg, 300 ⁇ mol) as described in Example 2b. Yield 33 mg (80 %).
  • reaction mixture was stirred at room temperature, after 1.5 hours another portion of triethylamine (75 ⁇ l, 0.54 mmol) and excess of cyclopropanesulfonyl chloride (50 ⁇ l, 0.49 mmol) was added. The reaction mixture was left over night at rooms temperature. Solvent was removed by evaporation and the residual material was purified by ⁇ PLC. Yield 18 mg (34%).
  • the subtitle compound was prepared following the procedure described in Exampel 1.s Starting from (lR,2S)-2-amino-l-(2,3-dihydrobenzo[b][l,4]dioxin-6-yl)propan-l-ol hydrochloride (43b, 1.46 g, 5.94 mmol), l-(4-fluorophenyl)-5-iodo-lH-indazole (2.4 g, 7.10 mmol), cesium carbonate (5.8 g, 17.80 mmol) and CuI (0.23 g, 1.21 mmol) in butyronitrile (18 mL). The reaction tube was capped and flushed with argon, the reaction mixture was stirred at +100 0 C for 16 hours. Final purification was made by HPLC. Yield0 1.16 g (36%)
  • the assay is based on a commercial kit from Panvera/Invitrogen (Part number P2893).
  • the assay technology is fluorescence polarization.
  • the kit utilises recombinant human GR (Panvera, Part number P2812), a FluoromoneTM labelled tracer (GS Red, Panvera, Part number P2894) and a Stabilizing Peptide 1OX (Panvera, Part number P2815).
  • the GR and Stabilizing Peptide reagents are stored at -70°C while the GS Red is stored at - 2O 0 C.
  • IM DTT Panvera, Part number P2325, stored at -20 0 C
  • GR Screening buffer 1OX Panvera, Part number P2814, stored at -7O 0 C initially but once thawed stored at room temperature). Avoid repeated freeze/thaws for all reagents.
  • the GR Screening buffer 1OX comprises 10OmM potassium phosphate, 20OmM sodium molybdate, ImM EDTA and 20% DMSO.
  • Test compounds (l ⁇ L) and controls (l ⁇ L) in 100% DMSO were added to black polystyrene 384-well plates (Greiner low volume black flat-bottom, part number 784076). 0% control was 100%DMSO and 100% control was lO ⁇ M Dexamethasone.
  • Background solution (8 ⁇ L; assay buffer 1OX, Stabilizing Peptide, DTT and ice cold MQ water) was added to the background wells.
  • GR solution (7 ⁇ L; assay buffer 10X, Stabilizing Peptide, DTT, GR and ice cold water) was added to all wells. The plate was sealed and incubated in a dark at room temperature for 2hours. The plate was read in an Analyst plate reader (LJL Biosystems/Molecular Devices Corporation) or other similar plate reader capable of recording fluorescence polarization (excitation wavelength 530nm, emission wavelength 59OnM and a dichroic mirror at 561nm). The IC50 values were calculated using XLf ⁇ t model 205.

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Abstract

Compounds of formula (I) or a pharmaceutically acceptable salt thereof; compositions comprising them, processes for preparing them and their use in medical therapy (for example modulating the glucocorticoid receptor in a warm blooded animal).

Description

Indozalyl sulphonamide derivatives useful as glucocorticoid modulators
The present invention relates to novel indazolyl sulphonamide derivatives, to pharmaceutical compositions comprising such derivatives, to processes for preparing such novel derivatives and to the use of such derivatives as medicaments (for example in the treatment of an inflammatory disease state).
Sulphonamide derivatives are disclosed as anti-inflammatories in WO 2004/019935 and WO 2004/050631. Pharmaceutically active sulphonamides are also disclosed in Arch. Pharm. (1980) 313 166-173, J.Med. Chem. (2003) 46 64-73, J. Med. Chem (1997) 40 996- 1004, EP 0031954, EP 1190710 (WO 200124786), US 5861401, US 4948809, US3992441 and WO 99/33786.
It is known that certain non-steroidal compounds interact with the glucocorticoid receptor (GR) and, as a result of this interaction, produce a suppression of inflammation (see, for example, US6323199). Such compounds can show a clear dissociation between anti-inflammatory and metabolic actions making them superior to earlier reported steroidal and non-steroidal glucocorticoids. The present invention provides further non-steroidal compounds as modulators (for example agonists, antagonists, partial agonists or partial antagonists) of the glucocorticoid receptor.
The present invention provides a compound of formula (I):
Figure imgf000002_0001
wherein:
A is Ci-ioalkyl, C5-10aryl,
Figure imgf000002_0002
C5.10heteroaryl, Cs-ioheteroarylCi-ealkyl, C5- 10arylCi_6alkoxy, Ci-iohaloalkyl, C3-7cycloalkyl, C3-7cycloalkylCi_4alkyl, Ci- 6alkyl0C(0)Ci.6alkyl, C1-6alkylC(O)OC1-6alkyl , C5.10aryloxyCMOalkyl or NR5R6C0.6alkyl whereby the aryl is optionally substituted with one or more substituents selected from B; R1 and Rla are independently selected from hydrogen, Ci-4alkyl, Ci-4haloalkyl, Ci- ^lydroxyalkyl and Ci^alkylOCi^alkyl; R2 is hydrogen or C1-4alkyl;
R3 is C3-7 cycloalkyl (optionally subtituted by halogen or C1-6 alkyl), Cs-ioarylCooalkyl, C5. ]oarylOCo-3alkyl,
Figure imgf000003_0001
Ci_6alkyl, Ci_6alkenyl or Ci-6alkynyl which are optionally substituted by one or more B; B is C0-3hydroxyalkyl, C1-4alkyl, C1-4alkoxy, C0_4alkylthioC0^alkyl, C3-6cycloalkylC0- 4thioalkyl, C0-3alkylS(0)nCo-4alkyl, Q-βhaloalkyl, C1-4haloalkoxy, halogen, nitro, cyano, CMalkylOCi.6alkyl, Q^alkylOCMalkylOCo^alkyl, C0-6alkylC(O)C0-6alkyl, C0- 4alkylC(O)OC0-4alkyl, C0-4alkylOC(0)Co.4alkyl, NR5R6C0.4alkyl, NR5R6C(O)C0-4alkyl, NR5R6OC(0)Co-4alkyl, NR5R6C(O)OC0-4alkyl, R6C(O)R5NC0-4alkyl, C0-4alkylOC(O)C0- 4alkylNH, Co-4alkylC(0)OCo-4alkylNH, C0-4alkylC(O)C0-4alkylNH or NR5R6S(O)nC0. 4alkyl;
R4 is hydrogen, hydroxy, halogen, C1-4 alkyl or C1-4 haloalkyl;
W is hydrogen, C3-7cycloalkyl,
Figure imgf000003_0002
phenyl, thienyl, isoxazolyl, pyrazolyl, pyridinyl or pyrimidinyl all optionally substituted with one or more substituents selected from halogen, Co-3hydroxyalkyl, d.4alkyl, C1-4alkoxy, Co^alkylthioCo^alkyl, C3-6cycloalkylCo- 4thioalkyl, Co-4alkylS(0)nCo-4alkyl, C1 ^haloalkyl, C1-4haloalkoxy, halo, nitro, cyano, C1- 4alkyl0C1-6alkyl, C1-6alkyl0C1.6alkyl0Ci.6alkyl, Co-6alkylC(0)C0.6alkyl, C0- 4alkylC(O)OC0-4alkyl, C0-4alkylOC(O)C0-4alkyl, NR5R6C0-4alkyl, NR5R6C(O)C0-4alkyl, NR5R6C(O)OC0-4alkyl, NR5R6OC(O)C0-4alkyl, R6C(O)R5NC0-4alkyl, C0-4alkylOC(O)C0- 4alkylNH, C0-4alkylC(O)OC0-4alkylNH, C0-4alkylC(O)C0-4alkylNH and NR5R6S(O)nC0. 4alkyl;
X is CH2, O, S, S(O), S(O)2 or NH;
Y is hydrogen, halogen, Ci-6 alkyl,
Figure imgf000003_0003
nitro, cyano, hydroxy, R5C(O), R5OC(O), R5C(O)O, S(O)nCi-4alkyl, R5R6NS(O)n, benzyloxy, imidazolyl, Ci-4alkylNHC(O), NR5R6C(O), CMalkylC(O)NH or NR5R6;
R5 and R6 are independently selected from hydrogen, Ci-4 alkyl and C3--7 cycloalkyl, or R5 and R6 form together a group -(O)C5.i0arylC(O)-; and n is 1 or 2, or a pharmaceutically acceptable salt thereof.
One embodiment relates to compound of formula (I), wherein: A is Ci-ioalkyl, Cs-ioaryl, Cs-ioarylCi.galkyl, C5_ioheteroaryl; Cs.ioheteroarylCi-δalkyl, C5- ioarylCi.6alkoxy, Ci-iohaloalkyl, C3-7cycloalkyl, C3-7cycloalkylCi_4alkyl, C1.
6alkyl0C(0)C1-6alkyl, Ci-6alkylC(O)OCi-6alkyl , C5-ioaryloxyCMoalkyl or NR5R6C0-6alkyl whereby the aryl is optionally substituted with one or more substituents selected from B; R1 and Rla are independently selected from hydrogen and Ci-4alkyl;
R2 is hydrogen;
R3 is C5-ioarylCo-3alkyl,
Figure imgf000004_0001
or C5- ioheteroary ICo-3 alkyl which are optionally substituted by one or more B;
B is Co^hydroxyalkyl, C1-4alkyl, C1-4alkoxy, Ci-όhaloalkyl, C1-4haloalkoxy, halogen or NR5R6C0-4alkyl;
R4 is hydrogen;
W is C3-7cycloalkyl, C1-4alkyl, phenyl or pyridinyl all optionally substituted with one or more substituents selected from halogen;
X is O, S or S(O)2; Y is hydrogen or halogen; and
R5 and R6 are independently selected from hydrogen and C1-4 alkyl, or R5 and R6 form together a group -(O)C5-10arylC(O)-, or a pharmaceutically acceptable salt thereof.
One embodiment relates to compound of formula (I), wherein:
A is Ci.ioalkyl, C1-10haloalkyl, C3-7cycloalkyl, C3-7cycloalkylC1-4alkyl,
Figure imgf000004_0002
βalkyl, Ci.6alkylC(O)OCi-6alkyl or NR5R6C0-6alkyl optionally substituted with one or more substituents selected from B.
R1 and Rla are independently selected from hydrogen and Ci-4alkyl; R2 is hydrogen;
R3 is Cs-ioarylCo-3alkyl,
Figure imgf000004_0003
which are optionally substituted by one or more B;
B is Co-3hydroxyalkyl, Ci^alkyl, Ci-4alkoxy, Ci.βhaloalkyl, Ci-4haloalkoxy, halogen or
NR5R6Co-4alkyl; R4 is hydrogen;
W is C3-7cycloalkyl,
Figure imgf000004_0004
phenyl or pyridinyl all optionally substituted with one or more substituents selected from halogen; X is O, S or S(O)2; Y is hydrogen or halogen; and
R5 and R6 are independently selected from hydrogen and Cj-4 alkyl, or R5 and R6 form together a group -(O)C5.10arylC(O)-, or a pharmaceutically acceptable salt thereof.
Another embodiment relates to compound of formula (I), wherein:
A is C5,ioaryl,
Figure imgf000005_0001
whereby the aryl is optionally substituted with one or more substituents selected from B; R1 and Rla are independently selected from hydrogen and C1-4alkyl;
R2 is hydrogen;
R3 is C5-iOarylCo.3alkyl, C5-i0arylOC0-3alkyl or
Figure imgf000005_0002
which are optionally substituted by one or more B;
B is C0_3hydroxyalkyl, Q^alkyl, C1-4alkoxy, Ci.ehaloalkyl, Ci-4haloalkoxy, halogen or NR5R6Co-4alkyl;
R4 is hydrogen;
W is C3.7cycloalkyl, C^alkyl, phenyl or pyridinyl all optionally substituted with one or more substituents selected from halogen;
X is O, S or S(O)2; Y is hydrogen or halogen; and
R5 and R6 are independently selected from hydrogen and C1-4 alkyl, or R5 and R6 form together a group -(O)C5-10arylC(O)-, or a pharmaceutically acceptable salt thereof.
A further embodiment relates to compound of formula (I), wherein:
A is C5-ioheteroaryl or Cs-ioheteroarylCi-όalkyl optionally substituted with one or more substituents selected from B;
R1 and Rla are independently selected from hydrogen and C1-4alkyl;
R2 is hydrogen; R3 is C5.ioarylCo-3alkyl, C5-1oarylOCo-3alkyl or Cs.ioheteroarylCo-salkyl which are optionally substituted by one or more B; B is C0-3hydroxyalkyl, C1-4alkyl, Ci-4alkoxy, Q-βhaloalkyl, Ci-4haloalkoxy, halogen or NR5R6C0_4alkyl; R4 is hydrogen;
W is C3-7cycloalkyl, Ci.4alkyl, phenyl or pyridinyl all optionally substituted with one or more substituents selected from halogen; X is O, S or S(O)2; Y is hydrogen or halogen; and
R5 and R6 are independently selected from hydrogen and C1-4 alkyl, or R5 and R6 form together a group -(O)C5-10arylC(O)-, or a pharmaceutically acceptable salt thereof.
One embodiment relates to compound of formula (I), wherein:
A is Ci-iOalkyl, C5-1OaTyI, Cs-^aryld-ealkyl, Cs.ioheteroaryl, Cs^oheteroarylC^alkyl, C5- ioaryld-όalkoxy, C^ohaloalkyl, C3-7cycloalkyl, C3-7cycloalkylC1-4alkyl, C1- 6alkyl0C(0)Ci.6alkyl, C1-6alkylC(O)OC1-6alkyl , C5-i0aryloxyCi-10alkyl or NR5R6C0-6alkyl whereby the aryl is optionally substituted with one or more substituents selected from B;
R1 and Rla are independently selected from hydrogen, C1-4alkyl, C1-4haloalkyl, C1-
^ydroxyalkyl and Ci-4alkyl0C1.4alkyl;
R2 is hydrogen or
Figure imgf000006_0001
R3 is C3-7 cycloalkyl (optionally subtituted by halogen or C1-6 alkyl), Cs-loarylCo-3alkyl, C5- ioarylOCo-3alkyl, C5-ioheteroarylCo-3alkyl,
Figure imgf000006_0002
or C].6alkynyl which are optionally substituted by one or more B;
B is Co-3hydroxyalkyl, C1-4alkyl, C1-4alkoxy, Co^alkylthioCo^alkyl, C3-6cycloalkylCo-
4thioalkyl, Co-3alkylS(0)nCo-4alkyl, Ci-βhaloalkyl,
Figure imgf000006_0003
halogen, nitro, cyano, C1-4alkyl0Ci-6alkyl, C0-6alkylOC1-4alkylOCo-4alkyl, C0-6alkylC(O)C0-6alkyl, C0.
4alkylC(O)OC0-4alkyl, C0-4alkylOC(O)C0-4alkyl, NR5R6C0-4alkyl, NR5R6C(O)C0-4alkyl,
NR5R6OC(O)C0-4alkyl, NR5R6C(O)OC0-4alkyl, R6C(O)R5NC0-4alkyl, C0-4alkylOC(0)Co.
4alkylNH, C0-4alkylC(O)OC0-4alkylNH, C0^alkylC(O)C0-4alkylNH Or NR5R6S(O)nC0-
4alkyl; R4 is hydrogen, hydroxy, halogen, C1-4 alkyl or Ci-4 haloalkyl;
W is C3-7cycloalkyl, Ci-4alkyl, phenyl, thienyl, isoxazolyl, pyrazolyl, pyridinyl or pyrimidinyl all optionally substituted with one or more substituents selected from halogen, Co-3hydroxyalkyl, Q^alkyl, Ci-4alkoxy, Co-4alkylthioCo-4alkyl, C3.6cycloalkylCo-4thioalkyl, Co-4alkylS(0)nCo-4alkyl,
Figure imgf000007_0001
Ci-4haloalkoxy, halo, nitro, cyano, Ci-4alkylOCi- 6alkyl,
Figure imgf000007_0002
C0-6alkylC(O)C0-6alkyl, C0.4alkylC(O)OC0-4alkyl, Co^alkylOC(0)C0-4alkyl, NR5R6C0-4alkyl, NR5R6C(O)C0-4alkyl, NR5R6C(O)OC0.4alkyl, s NR5R6OC(O)C0-4alkyl, R6C(O)R5NC0-4alkyl, C0-4alkylOC(O)C0-4alkylNH, C0- 4alkylC(O)OC0-4alkylNH, C0-4alkylC(O)C0-4alkylNH and NR5R6S(O)nC0-4alkyl; X is CH2, O, S, S(O), S(O)2 or NH;
Y is hydrogen, halogen, C1-6 alkyl, C1-4alkoxy, Ci-4thioalkyl, Ci-4haloalkyl, Ci-4alkoxyhalo, nitro, cyano, hydroxy, R5C(O), R5OC(O), R5C(O)O, S(O)nC1-4alkyl, R5R6NS(O)n, I0 benzyloxy, imidazolyl, Ci-4alkylNHC(O), NR5R6C(O),
Figure imgf000007_0003
or NR5R6;
R5 and R6 are independently selected from hydrogen, Ci-4 alkyl and C3-7 cycloalkyl, or R5 and R6 form together a group -(0)C5-ioarylC(0)-; and n is 1 or 2, or a pharmaceutically acceptable salt thereof.
15
One embodiment relates to compounds of formula I wherein
A is Cμioalkyl, C5-i0aryl,
Figure imgf000007_0004
C5- i0arylCi-6alkoxy, Ci-iohaloalkyl, C3-7cycloalkyl, C3-7CyClOaIlCyIC1 ^alkyl, Q- 6alkyl0C(0)C1-6alkyl, C1-6alkylC(O)OC1-6alkyl, C5-ioaryloxyCi-10alkyl or NR5R6Co-6alkylo whereby the aryl is optionally substituted with one or more substituents selected from B.
In a further embodiment A is C3-6cycloalkyl. In another embodiment A is cyclopropyl or cyclopentyl.
In a further embodiment A is C3-6cycloalkylCi-2alkyl. In yet another embodiment A is cyclopentyl-methyl. 5 In a further embodiment A is Cμioalkyl. In one embodiment A is methyl, ethyl, n-propyl, i- propyl, n-butyl, i-butyl, s-butyl, t-butyl, n-pentyl, i-pentyl, neo-pentyl, n-hexyl or i-hexyl.
In a further embodiment A is methyl. In another embodiment A is n-propyl. In yet another embodiment A is n-pentyl or s-pentyl. In yet a further embodiment A is s-butyl or n-butyl.
In another embodiment A is n-hexyl. 0 In one embodiment A is C^haloalkyl, In another embodiment A is fluoromethyl, difluoromethyl, trifluoromethyl, fluoroethyl, difluoroethyl, trifluoroethyl or trifluoropropyl. In another embodiment A is trifluoromethyl. In yet another embodiment A is trifluoromethyl, trifluoroethyl or trifluoropropyl.
In a further embodiment A is Cs-ioheteroarylCi-όalkyl. In one embodiment A is dioxoisoindolyl-ethyl.
In another mebodiment A is Cs-δheteroaryl optionally substituted with B. In a further embodiment A is imidazolyl or isooxazolyl optionally substituted with B. In a further embodiment A is imidazolyl or isooxazolyl substituted with one or two methyl. In one embodiment A is
Figure imgf000008_0001
optionally substituted with C]-4alkoxy. In yet a further embodiment A is phenyl-ethoxy substituted with methoxy.
In another embodiment A is C1-4alkylOC(0)CMalkyl. In one embodiment A is Q-
2alkyl0C(0)Ci-2alkyl. In another embodiment A is propanoate.
In one embodiment A is Cs.ioheteroaryl. In a further embodiment A is pyridinyl.
One embodiment relates to compounds of formula I wherein R1 and Rla are independently selected from hydrogen and C1-4alkyl. In another embodiment R1 is hydrogen. In another embodiment R1 is methyl. In yet a further embodiment Rla is hydrogen. In yet another embodiment R2 is hydrogen.
One embodiment relates to compounds of formula I wherein R3 is Cs.ioarylCo-salkyl, C5- 10arylOCo-3alkyl or Cs-ioheteroarylCooalkyl which are optionally substituted by one or more B. In a further embodiment R3 is Cs-earyl. In another embodiment R3 is phenyl. In a further embodiment R3 is phenyl substituted with B. In yet a further embodiment R3 is phenyl substituted with halogen or R5S(O)2, wherein R5 is d^alkyl. In one embodiment B is fluoro. In another embodiment B is methyl-S(O)2.
In yet a further embodiment R3 is Cs-6heteroaryl. In one embodiment R3 is pyridinyl substituted with B. In yet a further embodiment R3 is pyridinyl substituted with C1-4alkoxy. In one embodiment R3 is pyridinyl substituted with methoxy. In another embodiment R3 together with B form a dihydrobenzodioxinyl group as in example 43. One embodiment relates to compounds of formula I wherein R1 is C1.4a.kyl, Rla is hydrogen, R2 is hydrogen and R3 is Cs-ioaryl, whereb »yy aryl is optionally substituted by one or more B. In another embodiment B is halogen or R5S(O)2.
One embodiment relates to compounds of formula I wherein W is C3-7CyClOaIlCyI,
Figure imgf000009_0001
phenyl or pyridinyl all optionally substituted with one or more substituents selected from halogen. In a further embodiment W is C3-6CyClOaIlCyI, Ci-4alkyl, phenyl or pyridinyl optionally substituted with one substituent selected from halogen. In yet another embodiment W is phenyl. In another embodiment W is phenyl substituted with halogen. In a further embodiment W is phenyl substituted with fluoro.
In one embodiment W is cyclopentyl. In another embodiment W is iso-propyl. In yet another embodiment W is pyridinyl.
One embodiment relates to compounds of formula I wherein X is O. In another embodiment X is S. In a further embodiment X is S(O)2.
One embodiment relates to compounds of formula I wherein R1 is Q^alkyl, Rla is hydrogen, R is hydrogen, X is O and R is Cs-ioaryl, whereby aryl is optionally substituted by one or more B. In another embodiment B is halogen or R5S(O)2.
One embodiment relates to compounds of formula I wherein Y is hydrogen. In another embodiment Y is halogen. In a further embodiment Y is chloro.
One embodiment relates to compounds of formula I wherein R5 and R6 are independently selected from hydrogen and Ci-4 alkyl. In another embodiment R5 and R6 form together a group -(O)C5-10arylC(O)-.
Another embodiment relates to compounds of formula (IA)
(O
Figure imgf000009_0002
where A, R3 and W are as defined above, or a pharmaceutically acceptable salt thereof.
One embodiment relates to compounds of formula (I) wherein the group:
Figure imgf000010_0001
is bonded to the 5-position of the indazolyl ring system.
For the avoidance of doubt it is to be understood that where in this specification a group is qualified by 'hereinbefore defined', 'defined hereinbefore' or 'defined above' the said group encompasses the first occurring and broadest definition as well as each and all of the other definitions for that group.
For the avoidance of doubt it is to be understood that in this specification 'Co-β' means a carbon group having 0, 1, 2, 3, 4, 5 or 6 carbon atoms.
In this specification, unless stated otherwise, the term "alkyl" includes both straight and branched chain alkyl groups and may be, but are not limited to methyl, ethyl, n-propyl, i- propyl, n-butyl, i-butyl, s-butyl, t-butyl, n-pentyl, i-pentyl, neo-pentyl, n-hexyl or i-hexyl. The term having 1 to 4 carbon atoms and may be but are not limited to methyl, ethyl, n-propyl, i-propyl or tert-buty[. The term "Co" refers to a situtation where no carbon atom is present.
The term "alkoxy", unless stated otherwise, refers to radicals of the general formula -O-R, wherein R is selected from a hydrocarbon radical. The term "alkoxy" may include, but is not limited to methoxy, ethoxy, propoxy, isopropoxy, butoxy, t-butoxy, isobutoxy, cyclopropylmethoxy, allyloxy or propargyloxy.
In this specification, unless stated otherwise, the term "cycloalkyl" refers to an optionally substituted, partially or completely saturated monocyclic, bicyclic or bridged hydrocarbon ring system. The term "Ci-δcycloalkyl" may be, but is not limited to cyclopropyl, cyclobutyl, cyclopentyl or cyclohexyl.
In this specification, unless stated otherwise, the terms "halo" and "halogen" may be s fluorine, iodine, chlorine or bromine.
In this specification, unless stated otherwise, the term "haloalkyl" means an alkyl group as defined above, which is substituted with halogen as defined above. The term "C1- δhaloalkyl" may include, but is not limited to fluoromethyl, difluoromethyl, o trifluoromethyl, fluoroethyl, difluoroethyl or bromopropyl. The term "C1-3haloalkylO" may include, but is not limited to fluoromethoxy, difluoromethoxy, trifluoromethoxy, fluoroethoxy or difluoroethoxy.
In this specification, unless stated otherwise, the term "C5-1oaryl" refers to an aromatics group such as for example, phenyl or naphthyl.
In this specification, unless stated otherwise, the term "Cs.ioheteroaryl" refers to a mono- or bicyclic aromatic or partially aromatic ring containing one or more heteroatoms independently selected from nitrogen, oxygen, sulphur. Example of heteroaryls are oxazolyl, furyl, thienyl, pyrrolyl, pyrazolyl, imidazolyl, pyridinyl, pyrimidinyl, indolyl,o indazolyl, benzfuryl or benzthienyl. Heteroaryl may also be dihydrobenzodioxinyl, quinolinyl or isoquinolinyl.
When phenyl is substituted by OCH2O, OCH2CH2O or OCH2CH2 these groups link to adjacent carbons on the phenyl ring.
Cs.ioary 1C 1-4alkyl is for example benzyl. Cs-toarylCi^alkoxy is, for example a methoxy5 substituted with a phenyl. Ci-4alkoxyC5.1oaryl is, for example, a phenyl substituted with methoxy.
It will be appreciated that throughout the specification, the number and nature of substituents on rings in the compounds of the invention will be selected so as to avoid0 sterically undesirable combinations. Compounds of the present invention have been named with the aid of computer software (ACDLabs 8.0/Name(IUPAC)).
In another embodiment, the compounds of the invention are selected from N-[(l R,2S)- 1 -[ 1 -(4-fluorophenyl)indazol-5-yl]oxy- 1 -phenyl-propan-2- yljcyclopropanesulfonamide,
N-[(l R,2S)- 1 -[ 1 -(4-fluorophenyl)indazol-5-yl]oxy- 1 -phenyl-propan-2-yl]propane- 1 - sulfonamide,
N-[(l R,2S)- 1 -[ 1 -(4-fiuorophenyl)indazol-4-yl]oxy- 1 -phenyl-propan-2- yljcyclopropanesulfonamide,
N-[(lR,2S)-l-[l-(6-fluoropyridin-3-yl)indazol-5-yl]oxy-l-phenyl-propan-2- yl] cyclopropanesulfonamide,
N-[(lR,2S)-l-[l-(4-fluorophenyl)mdazol-5-yl]oxy-l-phenyl-propan-2- yl]methanesulfonamide, N-[( 1 R,2S)- 1 - [ 1 -(4-fruorophenyl)indazol-5-yl] oxy- 1 -phenyl-propan-2-yl]- 1 -phenyl- methanesulfonamide,
1,1,1 -trifluoro-N-[( 1 R,2S)- 1 -[ 1 -(4-fluorophenyl)indazol-5-yl]oxy- 1 -phenyl-propan-2- yl]methanesulfonamide,
5-[(l R,2S)-2-(dimethylsulfamoylamino)- 1 -phenyl-propoxy]- 1 -(4-fluorophenyl)indazole, N-[(1R,2S)- 1 -[ 1 -(4-fluorophenyl)indazol-5-yl]oxy- 1 -phenyl-propan-2-yl]propane-2- sulfonamide,
2-(l,3-dioxoisoindol-2-yl)-N-[(lR,2S)-l-[l-(4-fluorophenyl)indazol-5-yl]oxy-l-phenyl- propan-2-yl] ethanesulfonamide,
N- [( 1 R,2S)- 1 -[ 1 -(4-fluorophenyl)indazol-5-yl]oxy- 1 -phenyl-propan-2-yl]-3-(4- methoxyphenoxy)propane- 1 -sulfonamide,
N-[(l R,2S)- 1 -[ 1 -(4-fluorophenyl)indazol-5-yl]oxy- 1 -phenyl -propan-2- yl]ethanesulfonamide,
N-[(lR,2S)-l-[l-(4-fluorophenyl)indazol-5-yl]oxy-l-phenyl-propan-2-yl]pentane-2- sulfonamide, N-[(lR,2S)-l-[l-(4-fluorophenyl)indazol-5-yl]oxy-l-phenyl-propan-2-yl]butane-2- sulfonamide, N-[( 1 R,2S)- 1 -[ 1 -(4-fluorophenyl)indazol-5-yl]oxy- 1 -phenyl-propan-2-yl]butane-l - sulfonamide,
N-[(lR,2S)-l-[l-(4-fluorophenyl)indazol-5-yl]oxy-l-phenyl-propan-2-yl]-2-methyl- propane- 1 -sulfonamide, N- [( 1 R,2S)- 1 - [ 1 -(4-fluorophenyl)indazol-5-yl]oxy- 1 -phenyl-propan-2-yl]pentane- 1 - sulfonamide,
3,3,3-trifluoro-N-[(lR,2S)-l-[l-(4-fluorophenyl)indazol-5-yl]oxy-l-phenyl-propan-2- yl]propane- 1 -sulfonamide, methyl 3-[[(l R,2S)- 1 -[ 1 -(4-fluorophenyl)indazol-5-yl]oxy- 1 -phenyl-propan-2- yl]sulfamoyl]propanoate,
1 -cyclopentyl-N-[(l R,2S)- 1 -[ 1 -(4-fluorophenyl)indazol-5-yl]oxy- 1 -phenyl-propan-2- yljmethanesulfonamide,
N- [( 1 R,2S)- 1 - [ 1 -(4-fluorophenyl)indazol-5-yl]oxy- 1 -phenyl-propan-2- yl] cyclopentanesulfonamide, 2,2,2-trifluoro-N-[(lR,2S)-l-[l-(4-fluorophenyl)indazol-5-yl]oxy-l-phenyl-propan-2- yl]ethanesulfonamide, l-cyclohexyl-N-[(lR,2S)-l-[l-(4-fluorophenyl)indazol-5-yl]oxy-l-phenyl-propan-2- yl]methanesulfonamide,
N-[( 1 R,2S)- 1 -[ 1 -(4-fluorophenyl)indazol-5-yl]oxy- 1 -phenyl-propan-2-yl]hexane- 1 - sulfonamide,
N- [( 1 R,2S)- 1 -[ 1 -(4-fluorophenyl)indazol-5-yl]oxy- 1 -phenyl-propan-2-yl]pyridine-3- sulfonamide,
N-[I-[I -(4-fluorophenyl)indazol-5-yl] oxy-2 -methyl- 1 -phenyl-propan-2- yl] cyclopropanesulfonamide, N-[(lR,2S)-l-[l-(4-fluorophenyl)indazol-5-yl]oxy-l-(4-methylsulfanylphenyl)propan-2- yl] cyclopropanesulfonamide,
N-[(lS,2S)-l-[l-(4-fluorophenyl)indazol-5-yl]oxy-l-(4-methylsulfanylphenyl)propan-2- yl] cyclopropanesulfonamide,
N-[(l R,2S)- 1 -[ 1 -(4-fluorophenyl)indazol-5-yl]oxy- 1 -(4-methylsulfanylphenyl)propan-2- yl]cyclopropanesulfonamide,
N-[(lR,2S)-l-phenyl-l-(l-propan-2-ylindazol-5-yl)oxy-propan-2-yl]methanesulfonamide, N-[(lR,2S)-l-[l-(4-fluorophenyl)indazol-5-yl]oxy-l-(4-methylsulfmylphenyl)propan-2- yl]cyciopropanesulfonamide,
N-[(lR,2S)-l-(l-cyclopentylindazol-5-yl)oxy-l-phenyl-propan-2- yljcyclopropanesulfonamide, N-[(1R,2S)-1 -phenyl- l-(l-propan-2 -ylindazol-5-yl)oxy-propan-2- yljcyclopropanesulfonamide,
N-[(lR,2S)-l-[l-(4-fluorophenyl)indazol-5-yl]oxy-l-(4-methylsulfonylphenyl)propan-2- yljcyclopropanesulfonamide,
N-[(l R,2S)- 1 -[6-chloro-l -(4-fluorophenyl)indazol-5-yl]oxy- 1 -(4-fluorophenyl)propan-2- yl]cyclopropanesulfonamide,
N-[( 1R,2R)- 1 -[6-chloro- 1 -(4-fluorophenyl)indazol-5-yl]oxy- 1 -(4-fiuorophenyl)propan-2- yl]cyclopropanesulfonamide,
N-[2-[l-(4-fluorophenyl)indazol-5-yl]sulfanyl-2-phenyl-ethyl]cyclopropanesulfonamide,
N-[(lR,2S)-l-[l-(4-fluorophenyl)indazol-5-yl]sulfanyl-l-phenyl-propan-2- yljmethanesulfonamide,
N-[( 1 R,2S)- 1 -[ 1 -(4-fluorophenyl)indazol-5-yl]sulfonyl- 1 -phenyl-propan-2- yl]methanesulfonamide,
N-[(2R)-2-[l-(4-fluorophenyl)indazol-5-yl]oxy-2-phenyl-ethyl]cyclopropanesulfonamide,
N-[(2S)-2-[l-(4-fluorophenyl)indazol-5-yl]oxy-2-phenyl-ethyl]cyclopropanesulfonamide, N-[(lR,2S)-l-[l-(4-fluorophenyl)indazol-5-yl]oxy-l-quinolin-3-yl-propan-2- yljcyclopropanesulfonamide,
N-[(lR,2S)-l-(2,5-dioxabicyclo[4.4.0]deca-7,9,l l-trien-8-yl)-l-[l-(4- fluorophenyl)indazol-5-yl]oxy-propan-2-yl]cyclopropanesulfonamide,
Cyclopropanesulfonic acid N- { 1 -[6-methoxypyridin-3-yl]- 1 -[(1 -pyridin-2-yl- lH-indazol- 5-yl)oxy]propan-2-yl} amide,
Cyclopropanesulfonic acid N- { 1 -[6-methoxypyridin-3-yl] - 1 - [( 1 -pyridin-3 -yl- lH-indazol-
5-yl)oxy]propan-2-yl}amide,
Cyclopropanesulfonic acid N- { 1 -[2-methoxypyridin-4-yl]-l -[( 1 -pyridin-2-yl- lH-indazol-
5-yl)oxy]propan-2-yl} amide, Cyclopropanesulfonic acid N-{l-[2-methoxypyridin-4-yl]-l-[(l-pyridin-2-yl-lH-indazol-
5-yl)oxy]butan-2-yl} amide, 1 -Methyl- 1 H-imidazole-4-sulfonic acid N- [( 1 R,2S)- 1 - [ 1 -(4-fluoropheny l)indazol-5 - yl] oxy- 1 -pheny l-propan-2 -yl] amide, and
3,5-Dimethylisooxazole-4-sulfonic acid N-[(lR,2S)-l-[l-(4-fluorophenyl)indazol-5- yl]oxy- 1 -phenyl-propan-2-yl]amide, or a pharmaceutically acceptable salt thereof.
In another aspect the present invention provides the individual compound: N-[(l R,2S)- 1 -[ 1 -(4-fluorophenyl)indazol-5-yl]oxy- 1 -phenyl-propan-2- yljcyclopropanesulfonamide or a pharmaceutically acceptable salt thereof.
The compounds of formula (I) and pharmaceutically acceptable salts thereof may exist in solvated, for example hydrated or unsolvated forms, as well as cocrystalline forms and the present invention encompasses all such forms.
Compounds of formula (I) above may be converted to a pharmaceutically acceptable salt thereof, preferably an acid addition salt such as a hydrochloride, hydrobromide, phosphate, sulfphate, acetate, ascorbate, benzoate, fumarate, hemifumarate, furoate, succinate, maleate, tartrate, citrate, oxalate, xinafoate, methanesulphonate,/?-toluenesulphonate, benzenesulphonate, ethanesulphonate, 2-naphthalenesulfonate, mesytilenesulfonate, nitric acid, 1,5-naphthalene-disulphonate, p-xylenesulphonate, aspartate or glutamate.
They may also include basic addition salts such as an alkali metal salt for example sodium or potassium salts, an alkaline earth metal salt for example calcium or magnesium salts, a transition metal salt such as a zinc salt, an organic amine salt for example a salt of triethylamine, diethylamine, morpholine, iV-methylpiperidine, TV-ethylpiperidine, piperazine, procaine, dibenzylamine, N,N-dibenzylethylamine, choline or 2-aminoethanol or amino acids for example lysine or arginine.
Compounds of formula (I) may include an asymmetric centre and be chiral in nature. Where the compound is chiral, it may be in the form of a single stereoisomer, such as a enantiomer, or it may be in the form of mixtures of these stereoisomers in any proportions, including racemic mixtures. Therefore, all enantiomers, diastereomers, racemates and mixtures thereof are included within the scope of the invention. The various optical isomers may be isolated by separation of a racemic mixture of the compounds using conventional techniques, for example, fractional crystallisation, or HPLC. Alternatively the optical isomers may be obtained by asymmetric synthesis, or by synthesis from optically active starting materials.
Process
The compounds of formula (I) can be prepared using or adapting methods disclosed in the art, or by using or adapting the method disclosed in the Example below. Starting materials for the preparative methods are either commercially available or can be prepared by using or adapting literature methods.
One embodiment the present invention relates to a process for the preparation of compounds of formula (I) by coupling a compound of formula (II):
Figure imgf000016_0001
with a compound of formula (III):
Figure imgf000016_0002
wherein L1 is a leaving group (such as halogen (for example chloro) or mesylate or tosylate), in a suitable solvent (such as pyridine, THF or DMF), in the presence of a suitable base (such as a tri(Ci-6 alkyl)amine, for example diisopropylethylamine, or pyridine) and at a suitable temperature (such as -10 to 500C).
One embodiment the present invention relates to a process for the preparation of compounds of formula (II), wherein X is O, S or NH, (a) by coupling a compound of formula (IV)
Figure imgf000017_0001
wherein R4, W and Y are defined as in compounds of formula (I) and L2 is a leaving group (such as halogen or triflate) with a compound of formula (V)
Figure imgf000017_0002
wherein R1, Rla and R2 are defined as in compounds of formula (I) and G corresponds to R3 or a protected precurser to R3 and whereby the reaction can be performed in a suitable solvent (such as an aromatic solvent, for example toluene) or a polar, aprotic solvent, such as DMF or butyronitril, in the presence of a suitable base (such as a alkali metal alkoxide (for example sodium tert-butoxide) or, cesium carbonate, preferable mediated by a suitable metal catalyst such as Copper(I) iodide at a suitable temperature (for example in the range 80° to 12O0C), or, (b) reacting a compound of formula (VTI)
Figure imgf000017_0003
with a compound of formula (VIII)
Figure imgf000017_0004
wherein R1, R2, R4, X, W and Y are defined as in compounds of formula (I), G corresponds to R3 or a protected precurser to R3 and L3 is a leaving group (such as halogen, mesylate or tosylate), and whereby the reaction can be performed in a suitable solvent (such as DCM, DMF or acetonitrile), in the presence of a suitable base (such as an alkali metal carbonate, for example cesium carbonate or potassium carbonate) at a suitable temperature (for example in the range -10 to 5O0C), followed by a subsequent reductive amination step using or adopting literature methods, or,
(c) by reaction of a compound of formula (VIII) and a compound of formula (IX)
Figure imgf000018_0001
wherein R1, Rla, R2 and R3 are defined as in compounds of formula (I) and PG is a suitable protecting group such as BOC, Ms, Ns, Ts or related carbonyl-or sulfonyl residues, and whereby the reaction can be performed in a suitable solvent such as DCM or toluene in the presence of a suitable base such as NaH or KOtBu, followed by a deprotection step using or adopting literature methods.
As a specific case of a compound of formula (V), a compound of formula (X) might be used to prepare a compound of formula (II)
Figure imgf000018_0002
wherein R1, R2 and G are defined as in compounds of formula (V).
Compounds of formula (X) may be prepared by reacting a nucleophile G-M with a carbonyl compound of formula (XI) followed reduction and subsequent deprotection of the intermediate of formula (XII)
Figure imgf000018_0003
wherein R1, Rla are defined as in compounds of formula (I) and G corresponds to R3 or a protected precurser to R3 and L is a leaving group (such as alkoxy, methoxy(methyl)amino). M is a metal such as Li or Mg-halide. The addition of the nucleophile may be performed in a suitable aprotic solvent such as THF at moderate temperature between -10 and 5O0C. The following reduction and deprotection steps might be carried out by using or adopting literature methods.
Alternatively, compounds of formula (X) may be prepared by a reaction of a nuceophile G- M with an aldehyde of formula (XIII) and a subsequent deprotection.
Figure imgf000019_0001
wherein R1, Rla are defined as in compounds of formula (I) and are defined as in compounds of formula (I), G corresponds to R3 or a protected precurser to R3 and PG is a protecting group or hydrogen. M is a metal such as an alkali metal (e.g. Li) or Mg-halide. The reaction may be performed by following disclosed protocols for addition of carbanions to aldehydes.
Another way to prepare a compound of formula (X) is the reaction of nitroalkyles of formula (XIV) with aldehydes of formula (XV), followed by reduction of the nitro function
Figure imgf000019_0002
wherein R1 and Rla are defined as in compounds of formula (I), G corresponds to R3 or a protected precurser to R3 and PG is a protecting group or hydrogen. Both steps may be carried out by following or adopting literature methods.
The invention relates to processes for the preparation of the compounds of formula (I) as well as the intermediates.
Medical use Because of their ability to bind to the glucocorticoid receptor, the compounds of formula (I) are useful as anti-inflammatory agents, and can also display antiallergic, immunosuppressive and anti-proliferative actions. Thus, a compound of formula (I), or a pharmaceutically acceptable salt thereof can be used as a medicament for the treatment or prophylaxis of one or more of the following pathologic conditions (disease states) in a mammal (such as a human):
(i) Lung diseases, which coincide with inflammatory, allergic and/or proliferative processes: 5 • chronically obstructive lung diseases of any origin, mainly bronchial asthma
• bronchitis of different origins
• all forms of restructive lung diseases, mainly allergic alveolitis
• all forms of pulmonary edema, mainly toxic pulmonary edema
• sarcoidoses and granulomatoses, such as Boeck's disease o (ii) Rheumatic diseases/auto-immune diseases/degenerative joint diseases, which coincide with inflammatory, allergic and/or proliferative processes:
• all forms of rheumatic diseases, especially rheumatoid arthritis, acute rheumatic fever, polymyalgia rheumatica, collagenoses
• reactive arthritis s • inflammatory soft-tissue diseases of other origins
• arthritic symptoms in degenerative joint diseases (arthroses)
• traumatic arthritides
• collagen diseases of other origins, for example systemic lupus erythematodes, sclerodermia, polymyositis, dermatomyositis, polyarteritis nodosa, temporalo arteritis
• Sjogren's syndrome, Still syndrome, Felty's syndrome
(iii) Allergies, which coincide with inflammatory, allergic and/or proliferative processes:
• All forms of allergic reactions, for example Quincke's edema, hay fever, insect bites, allergic reactions to pharmaceutical agents, blood derivatives, contrastS media, etc., anaphylactic shock, urticaria, contact dermatitis
(iv) Dermatological diseases, which coincide with inflammatory, allergic and/or proliferative processes:
• atopic dermatitis (mainly in children)
• psoriasis 0 • erythematous diseases, triggered by different noxae, for example radiation, chemicals, burns, etc. • acid bums
• bullous dermatoses
• diseases of the lichenoid group
• itching (for example of allergic origins) »all forms of eczema, such as, for example, atopic eczema or seborrheal eczema
•rosacea
• pemphigus vulgaris
• erythema exudativum multiforme
• erythema nodosum "balanitis
• vulvitis
• inflammatory hair loss, such as alopecia areata
• cutaneous T-cell lymphoma
(v) Nephropathies, which coincide with inflammatory, allergic and/or proliferative processes:
• nephrotic syndrome
• all nephritides
(vi) Liver diseases, which coincide with inflammatory, allergic and/or proliferative processes: • acute liver cell decomposition
• acute hepatitis of different origins, for example virally-, toxically- or pharmaceutical agent-induced
• chronically aggressive and/or chronically intermittent hepatitis
(vii) Gastrointestinal diseases, which coincide with inflammatory, allergic and/or proliferative processes:
• regional enteritis (Crohn's disease)
• ulcerative colitis
• gastroenteritis of other origins, for example native sprue
(viii) Proctological diseases, which coincide with inflammatory, allergic and/or proliferative processes:
• anal eczema • fissures
• haemorrhoids
• idiopathic proctitis
(ix) Eye diseases, which coincide with inflammatory, allergic and/or proliferative processes:
• allergic keratitis, uvenitis iritis
• conjunctivitis
• blepharitis
• optic neuritis • chorioiditis
• sympathetic ophthalmia
(x) Diseases of the ear-nose-throat area, which coincide with inflammatory, allergic and/or proliferative processes:
• allergic rhinitis, hay fever • otitis externa, for example caused by contact dermatitis, infection, etc.
• otitis media
(xi) Neurological diseases, which coincide with inflammatory, allergic and/or proliferative processes:
• cerebral edema, mainly tumor-induced cerebral edema • multiple sclerosis
• acute encephalomyelitis
• different forms of convulsions, for example infantile nodding spasms
(xii) Blood diseases, which coincide with inflammatory, allergic and/or proliferative processes: • acquired haemo lytic anemia
• idiopathic thrombocytopenia
(xiii) Tumor diseases, which coincide with inflammatory, allergic and/or proliferative processes:
• acute lymphatic leukaemia • malignant lymphoma
• lymphogranulomatoses • lymphosarcoma
• extensive metastases, mainly in breast and prostate cancers
(xiv) Endocrine diseases, which coincide with inflammatory, allergic and/or proliferative processes: • endocrine orbitopathy
•thyrotoxic crisis
• de Quervain's thyroiditis
• Hashimoto's thyroiditis
• hyperthyroidism (xv) Transplants, which coincide with inflammatory, allergic and/or proliferative processes;
(xvi) Severe shock conditions, which coincide with inflammatory, allergic and/or proliferative processes, for example anaphylactic shock
(xvii) Substitution therapy, which coincides with inflammatory, allergic and/or proliferative processes, with:
• innate primary suprarenal insufficiency, for example congenital adrenogenital syndrome
• acquired primary suprarenal insufficiency, for example Addison's disease, autoimmune adrenalitis, meta-infective, tumors, metastases, etc. • innate secondary suprarenal insufficiency, for example congenital hypopituitarism
• acquired secondary suprarenal insufficiency, for example meta-infective, tumors, etc. (xviii) Emesis, which coincides with inflammatory, allergic and/or proliferative processes:
• for example in combination with a 5-HT3-antagonist in cytostatic-agent-induced vomiting.
Without prejudice to the foregoing, the compounds of formula (I) can also be used to treat disorders such as: Conies Syndrome, primary and secondary hyperaldosteronism, increased sodium retention, increased magnesium and potassium excretion (diuresis), increased water retention, hypertension (isolated systolic and combined systolic/diastolic), arrhythmias, myocardial fibrosis, myocardial infarction, Bartter's Syndrome, disorders associated with excess catecholamine levels, diastolic and systolic congestive heart failure (CHF), peripheral vascular disease, diabetic nephropathy, cirrhosis with edema and ascites, oesophageal varicies, Addison's Disease, muscle weakness, increased melanin pigmentation of the skin, weight loss, hypotension, hypoglycemia, Cushing's Syndrome, obesity, hypertension, glucose intolerance, hyperglycemia, diabetes mellitus, osteoporosis, polyuria, polydipsia, inflammation, autoimmune disorders, tissue rejection associated with organ transplant, malignancies such as leukemias and lymphomas, acute adrenal insufficiency, congenital adrenal hyperplasia, rheumatic fever, polyarteritis nodosa, granulomatous polyarteritis, inhibition of myeloid cell lines, immune proliferation/apoptosis, HPA axis suppression and regulation, hypercortisolemia, modulation of the Thl/Th2 cytokine balance, chronic kidney disease, stroke and spinal cord injury, hypercalcemia, hyperglycemia, acute adrenal insufficiency, chronic primary adrenal insufficiency, secondary adrenal insufficiency, congenital adrenal hyperplasia, cerebral edema, thrombocytopenia, and Little's syndrome, systemic inflammation, inflammatory bowel disease, systemic lupus erythematosus, discoid lupus erythematosus, polyartitis nodosa, Wegener's granulomatosis, giant cell arthritis, rheumatoid arthritis, osteoarthritis, hay fever, allergic rhinitis, contact dermatitis, atopic dermatitis, exfoliative dermatitis, urticaria, angioneurotic edema, chronic obstructive pulmonary disease, asthma, tendonitis, bursitis, Crohn's disease, ulcerative colitis, autoimmune chronic active hepatitis, hepatitis, cinhosis, inflammatory scalp alopecia, panniculitis, psoriasis, inflamed cysts, pyoderma gangrenosum, pemphigus vulgaris, bullous pemphigoid, dermatomyositis, eosinophilic fasciitis, relapsing polychondritis, inflammatory vasculitis, sarcoidosis Sweet's disease, type 1 reactive leprosy, capillary hemangiomas, lichen planus, erythema nodosum acne, hirsutism, toxic epidermal necrolysis, erythema multiform, cutaneous T-cell lymphoma, psychoses, cognitive disorders (such as memory disturbances) mood disorders (such as depression and bipolar disorder), anxiety disorders and personality disorders.
As used herein the term "congestive heart failure" (CHF) or 'congestive heart disease" refers to a disease state of the cardiovascular system whereby the heart is unable to efficiently pump an adequate volume of blood to meet the requirements of the body's tissues and organ systems. Typically, CHF is characterized by left ventricular failure (systolic dysfunction) and fluid accumulation in the lungs, with the underlying cause being attributed to one or more heart or cardiovascular disease states including coronary artery disease, myocardial infarction, hypertension, diabetes, valvular heart disease, and cardiomyopathy. The term "diastolic congestive heart failure" refers to a state of CHF characterized by impairment in the ability of the heart to properly relax and fill with blood. Conversely, the term "systolic congestive heart failure" refers to a state of CHF characterized by impairment in the ability of the heart to properly contract and eject blood. As will be appreciated by one of skill in the art, physiological disorders may present as a "chronic" condition, or an "acute" episode. The term "chronic", as used herein, means a condition of slow progress and long continuance. As such, a chronic condition is treated when it is diagnosed and treatment continued throughout the course of the disease. Conversely, the term "acute"means an exacerbated event or attack, of short course, followed by a period of remission. Thus, the treatment of physiological disorders contemplates both acute events and chronic conditions. In an acute event, compound is administered at the onset of symptoms and discontinued when the symptoms disappear.
In another aspect the present invention provides the use of a compound of formula (I), or a pharmaceutically acceptable salt thereof, for use in therapy (such as a therapy described above).
In yet another aspect the present invention provides the use of a compound of formula (I), or a pharmaceutically acceptable salt thereof, in the manufacture of a medicament for use in the treatment of a glucocorticoid receptor mediated disease state (such as a disease state described above). In a further aspect the invention provides the use of a compound of formula (I), or a pharmaceutically acceptable salt thereof, in the manufacture of a medicament for use in the treatment of an inflammatory (such as an arthritic) condition.
In a still further aspect the invention provides the use of a compound of formula (I), or a pharmaceutically acceptable salt thereof, in the manufacture of a medicament for use in the treatment of an asthmatic condition.
In another aspect the invention provides the use of a compound of formula (I), or a pharmaceutically acceptable salt thereof, in the manufacture of a medicament for use in the treatment of COPD.
The present invention further provides a method of treating a glucocorticoid receptor mediated disease state, an inflamatory condition, an asthmatic condition and/or COPD, in a mammal (such as man), which comprises administering to a mammal in need of such treatment an effective amount of a compound of formula (I), or a pharmaceutically acceptable salt thereof.
In the context of the present specification, the term "therapy" also includes "prophylaxis" unless there are specific indications to the contrary. The terms "therapeutic" and "therapeutically" should be construed accordingly.
In this specification, unless stated otherwise, the terms "inhibitor" and "antagonist" mean a compound that by any means, partly or completely, blocks the transduction pathway leading to the production of a response by the agonist. The term 'agonist' mean a compound that by any means, partly or completely, provokes the transduction pathway leading to the production of a response.
The term "disorder", unless stated otherwise, means any condition and disease associated with glucocorticoid receptor activity.
Pharmaceutical composition
In order to use a compound of formula (I), or a pharmaceutically acceptable salt thereof, for the therapeutic treatment of a mammal, said active ingredient is normally formulated in accordance with standard pharmaceutical practice as a pharmaceutical composition. Therefore in another aspect the present invention provides a pharmaceutical composition comprising a compound of formula (I), or a pharmaceutically acceptable salt thereof, (active ingredient) and a pharmaceutically acceptable adjuvant, diluent or carrier. In a further aspect the present invention provides a process for the preparation of said composition comprising mixing the active ingredient with a pharmaceutically acceptable adjuvant, diluent or carrier. Depending on the mode of administration, the pharmaceutical composition can comprise from 0.05 to 99 %w (per cent by weight), for example from 0.05 to 80 %w, such as from 0.10 to 70 %w (for example from 0.10 to 50 %w), of active ingredient, all percentages by weight being based on total composition.
A pharmaceutical composition of the present invention can be administered in a standard manner for the disease condition that it is desired to treat, for example by topical (such as to the lung and/or airways or to the skin), oral, rectal or parenteral administration. Thus, a the compound of formula (I), or a pharmaceutically acceptable salt thereof, may be formulated into the form of, for example, an aerosol, a powder (for example dry or dispersible), a tablet, a capsule, a syrup, a granule, an aqueous or oily solution or suspension, an (lipid) emulsion, a suppository, an ointment, a cream, drops, or a sterile injectable aqueous or oily solution or suspension.
A suitable pharmaceutical composition of this invention is one suitable for oral administration in unit dosage form, for example a tablet or capsule containing between 0.1 mg and 1 g of active ingredient.
In another aspect a pharmaceutical composition of the invention is one suitable for intravenous, subcutaneous, intraarticular or intramuscular injection.
Buffers, pharmaceutically-acceptable cosolvents such as polyethylene glycol, polypropylene glycol, glycerol or ethanol or complexing agents such as hydroxy-propyl β- cyclodextrin may be used to aid formulation.
The above formulations may be obtained by conventional procedures well known in the pharmaceutical art. Tablets may be enteric coated by conventional means, for example to provide a coating of cellulose acetate phthalate.
The invention further relates to combination therapies or compositions wherein a GR agonist of formula (I), or a pharmaceutically acceptable salt thereof, or a pharmaceutical composition comprising a GR agonist of formula (I), or a pharmaceutically acceptable salt thereof, is administered concurrently (possibly in the same composition) or sequentially with one or more agents for the treatment of any of the above disease states.
For example, for the treatment of rheumatoid arthritis, osteoarthritis, COPD, asthma or allergic rhinitis a GR agonist of the invention can be combined with one or more agents for the treatment of such a condition. Where such a combination is to be administered by inhalation, then the one or more agents is selected from the list comprising:
• a PDE4 inhibitor including an inhibitor of the isoform PDE4D;
• a selective β.sub2. adrenoceptor agonist such as metaproterenol, isoproterenol, isoprenaline, albuterol, salbutamol, formoterol, salmeterol, terbutaline, orciprenaline, bitolterol mesylate, pirbuterol or indacaterol; • a muscarinic receptor antagonist (for example a Ml, M2 or M3 antagonist, such as a selective M3 antagonist) such as ipratropium bromide, tiotropium bromide, oxitropium bromide, pirenzepine or telenzepine; • a steroid (such as budesonide);
• a modulator of chemokine receptor function (such as a CCRl receptor antagonist); or,
• an inhibitor of p38 kinase function. s In another embodiment of the invention where such a combination is for the treatment of COPD, asthma or allergic rhinitis, the GR agonist of formula (I), or a pharmaceutically acceptable salt thereof, can be administered by inhalation or by the oral route and this is in combination with a xanthine (such as aminophylline or theophylline) which can be administered by inhalation or by the oral route. The GR agonist of formulao (I) and xanthine may be administered together. They may be administered sequencially. Or they may be administered separately.
Examples
The following Examples illustrate the invention. The following abbreviations are used ins the Examples:
TFA Trifluoroacetic acid;
THF Tetrahydrofuran
DCM Dichloromethane
HPLC High Performance Liquid Chromatography; o LC/MS Liquid Column Chromatography / Mass Spectroscopy;
GC Gas Chromatography
DMSO Dimethylsulfoxide;
APCI-MS Atmospheric Pressure Chemical Ionisation Mass Spectroscopy; r.t. Room temperature, which is a temperature in the range from of 16°C to 250C5
General Methods
NMR spectra were recorded on a Varian Mercury- VX 300 MHz instrument or a Varian Inova 400MHz instrument. The central peaks of chloroform-d (H 7.27 ppm), acetone (H 2.05 ppm), dichloromethane-d2 (H 5.32 ppm)or DMSO-cfc (H 2.50 ppm) were used as0 internal references.
The following method was used for LC/MS analysis: Instrument Agilent 1100; Column Waters Symmetry 2.1 x 30 mm; Mass APCI; Flow rate 0.7 mL/min; Wavelength 254 nm; Solvent A: water + 0.1% TFA; Solvent B: acetonitrile + 0.1% TFA ; Gradient 15-95%/B 2.7 min, 95% B 0.3 min. The following method was used for GC-MS analysis: s Low resolution mass spectra and accurate mass determination were recorded on a Hewlett- Packard GC. MS system equipped with EI ionisation chamber, 7OeV. The following method was used for LC analysis:
Method A. Instrument Agilent 1100; Column: Kromasil C18 100 x 3 mm, 5μ particle size, Solvent A: 0.1 %TF A/water, Solvent B: 0.08%TFA/acetonitrile Flow: 1 mL/min, o Gradient 10-100%/B 20 min, 100% B 1 min. Absorption was measured at 220, 254 and 280 nm.
A Kromasil KR-100-5-C18 column (250 x 20 mm, Akzo Nobel) and mixtures of acetonitrile/water (0.1% TFA) at a flow rate of 10 mL/min was used for preparative HPLC.s Unless stated otherwise, starting materials were commercially available. All solvents and commercial reagents were of laboratory grade and were used as received.
Example 1
N-[(lR,2S)-l~[l-(4-fluorophenyl)indazol-5-yl]oxy-l-phenyl-propan-2-0 yl] cyclopropanesulfonamide
Figure imgf000029_0001
(lR,2S)-l-[l-(4-Fluorophenyl)indazol-yl]oxy-l-phenyl-propan-2-amine (Ia, 16 mg, 0.044 mmole) was dissolved in dry pyridine (2 mL) and cooled to 0 °C. Cyclopropanesulfonyl chloride (16 μl, 0.056 mmole) was added and the mixture was stirred at room temperature for 45 min. The progression of the reaction was followed by HPLC (R.P C- 18, 20-90% gradient Of CH3CN in water, 0.1% TFA). After 45 and 75 min additional portions of cyclopropanesulfonyl chloride (5 and 6 μl respectively) were added. As the reaction proceeded very slowly, triethylamine (27 μl, 0.2 mmol) was added after a total of 2.5 h of stirring. The stirring was continued at ambient temperature for additional 18.5h and the reaction mixture was then partitioned between ethyl acetate and aqueous hydrochloric acid (1.7M). The organic phase was washed twice with aqueous hydrochloric acid (1.7M), then with water and finally with brine. Evaporation and flash chromatography (SiO2, gradient of 0-50% EtOAc in
5 heptane) and finally lyophilization from dioxane gave the title compound (7 mg, 33%) containing 15 mol% of dioxane.
1H-NMR (300 MHz, DMSO-d6) δ 8.16 (lH,s), 7.76-7.73 (2H, m), 7.70 (IH, d), 7.44-7.34 (7H, m), 7.28 (IH, t, further coupled), 7.11 (IH, d)7.24 (IH, dd), 5.33 (IH, d), 3.73 (IH, m), 2.39- 2.33 (IH, m), 1.24 (3H, d), 0.89-0.79 (4H, m). o 19F-NMR (DMSO-de): -115.8 (tt, unresolved) APCI-MS m/z: 466.0 [MH+].
(lR,2S)-l-[l-(4-Fluorophenyl)indazol-5-yl]oxy-l-phenyl-propan-2-amine (Ia)
Figure imgf000030_0001
s The sub-title compound was prepared essentially by a method described by Job &
Buchwald: Org. Lett. 2002, 4 (21), 3703-3706. l-(Fluorophenyl)-5-iodoindazole (43 mg, 0.12 mmole), (lR,2S)-norephedrine (16 mg, 0.1 mmol), copper (I) iodide (2.2 mg, 5 mol%) and cesium carbonate (84 mg, 0.26 mmole) were suspended in butyronitrile (1 ml). The reaction vessel was capped and the mixture was stirredo at 125 °C. The progress of the reaction was followed by HPLC (R.P C-18, 20-90% gradient of CH3CN in water, 0.1% TFA). After 7.5h additional (lR,2S)-norephedrine (70 mg), copper (I) iodide (16 mg) and cesium carbonate (136 mg) were added and the stirring was continued at 125 °C. After 2h all l-(fluorophenyl)-5-iodoindazole hed been consumed and the mixture was cooled, filtered and evaporated. Flash chromatography (SiO2, gradient of 0-30% MeOH in5 EtOAc) gave the sub-title compound (19 mg, 41%).
1H-NMR (300 MHz, DMSO^+ D2O, TFA added) δ 8.16 (IH, d), 7.76-7.68 (3H, m), 7.43- 7.28 (8H, m), 7.12 (IH, d), 5.64 (IH, d), 3.70 (IH, qd), 1.16 (3H, d). 19F-NMR (DMSO-d6): δ -115.97 (tt, unresolved). APCI-MS m/z: 362.2 [MH+].
Example 2
N-[(lR,2S)-l-[l-(4-fluorophenyl)indazol-5-yl]oxy-l-phenyl-propan-2-yl]propane-l- sulfonamide
Chiral
Figure imgf000031_0001
To a stirred solution of (l/?,21S)-l-[l-(4-fluorophenyl)indazol-5-yl]oxy-l-phenyl-propan-2- amine (Ia, 18 mg, 50 μmol) in acetonitrile (1 ml) was added triethylamine (100 μl), followed by propane- 1-sulfonyl chloride (21 mg, 150 μmol). Stirring was continued overnight. The mixture was then diluted with water (300 μl), and the title compound isolated by preparative HPLC to afford 23 mg (83 %) as white solid. 1H NMR (400 MHz, ^-acetone) δ 8.04 (d, J= 0.7 Hz, IH), 7.77 (m, 2H), 7.71 (d, J- 9.2 Hz, IH), 7.49 (d, J= 7.3 Hz, 2H), 7.41 - 7.29 (m, 5H), 7.27 (dd, J = 9.1, 2.4 Hz, IH), 7.16 (d, J= 2.3 Hz, IH), 6.25 (d, J= 9.0 Hz, IH), 5.47 (d, J= 4.2 Hz, IH), 3.89 (m, IH), 2.86 (m, 2H), 1.67 (m, 2H), 1.33 (d, J = 6.7 Hz, 3H), 0.92 (t, J = 7. APCI-MS m/z: 468 [MH+]
Example 3
N-[(lR,2S)-l-[l-(4-fluorophenyl)indazol-4-yl]oxy-l-phenyl-propan-2- yljcyclopropanesulfonamide
Figure imgf000031_0002
Prepared according the protocol described for Example 1 using (lR,2S)-l-[l-(4- fluorophenyl)indazol-4-yl]oxy-l-phenyl-propan-2-amine (Ia, 30 mg, 0.08 mmol) and cyclopropylsulphonyl chloride (22 μl, 0.21 mmol). Yield: 20 mg (53 %). 1H NMR (400 MHz, DMSO-J6) δ 8.51 (s, IH), 7.77 (td, J= 8.7, 3.8 Hz, 2H), 7.49 (d, J = 8.5 Hz, IH), 7.44 - 7.35 (m, 6H), 7.31 - 7.20 (m, 3H), 6.40 (d, J= 6.9 Hz, IH), 5.50 (d, J = 4.4 Hz, IH), 3.85 - 3.77 (m, IH), 2.43 - 2.35 (m, IH), 1.31 (d, J= 6.7 Hz, 3H), 0.90 - 0.79 (m, 4H). APCI-MS: 466 m/z [MH+]
(lR,2S)-l-[(4-Fluorophenyl)indazol-4-yl]oxy-l-phenyl-propan-2-amine (3a) Prepared according to the protocol described for Ia starting from 4-iodo-(4- fluorophenyl)indazole (3b, 332 mg, 0.98 mmol) and (lR,2S)-norephedrine (742 mg, 4.9 mmol). Yield: 150 mg (42%). APCI-MS: 362 m/z [MH+]
4-Iodo-(4-fluorophenyl)indazole (3b)
4-Bromo-(4-fluorophenyl)indazole (291 mg, 1 mmol), copper(I)iodide (9.5 mg, 0.05 mmol), sodium iodide (300mg, 2 mmol) and (lR,2R)-(N,N'-dimethylcyclohexane-l,2- diamine (14.2 mg, 0.1 mmol) in dioxane (1 ml) were mixed and stirred in argon at 110°C.
All starting material was consumed after 24 h. The mixture was cooled and ammonia (5 ml, 28% in water) and water (20 ml) were added followed by extraction with DCM (2 x 15 ml). The organic phase was concentrated and the crude product was purified by flash chromatography (EtO Ac/Heptane) to give the title compound (322 mg, 98%).
APCI-MS m/z: 339 [MH+].
Example 4
N-[(lR,2S)-l-[l-(6-fluoropyridin-3-yl)indazol-5-yl]oxy-l-phenyl-propan-2- yl] cyclopropanesulfonamide
Figure imgf000032_0001
Prepared according the protocol descibed for Example 1 using l-[(6-fluoro-l-pyridin-3- yl)indazol-5-yl]oxy-l-phenyl-propan-2-amine (20 mg, 0.06 mmol) and cyclopropylsulphone chloride (18 μl, 0.17 mmol). Yield: 5 mg (71%). 1H NMR (400 MHz, DMSO-J5) δ 8.63 (s, IH), 8.36 (ddd, J= 9.1, 6.8, 2.6 Hz, IH), 8.24 (s, IH), 7.78 (d, J= 9.2 Hz, IH), 7.45 - 7.34 (m, 6H), 7.30 - 7.25 (m, 2H), 7.14 (d, J= 2.3 Hz, IH), 5.34 (d, J = 4.4 Hz, IH), 3.76 - 3.70 (m, IH), 2.40 - 2.31 (m, IH), 1.24 (d, J = 6.9 Hz, 3H), 0.88 - 0.80 (m, 4H) 5 APCI-MS: 466 m/z [MH+]
1 -[(6-fluoro- 1 -pyridin-3-yl) indazo l-5-yl]oxy-l-phenyl-propan-2-am ine (4b) 4-Iodo-(6-fluoro-l-pyridin-3-yl)indazole (4c, 191 mg, 0.56 mmol), (lR,2S)-norephedrine (426 mg, 2.8 mmol), copper(I)iodide (139 mg, 0.73 mmol) and cesium carbonate (1.8 g, io 5.6 mmol) in butyronitrile (3 ml) were mixed and stirred in argon at 125°C for 2h. The starting material was consumed according to LC-MS. The crude mixture was purified by flash chromatography (EtOAc/heptane/methanol) followed by preparative HPLC (MeCN/water/l%TFA) to obtain the title compound (20 mg, 10 %). APCI-MS m/z: 363 [MH+].
I5
4-Iodo-(6-fluoro-l-pyridin-3-yl)indazole (4c)
4-Bromo-(6-fluoro-l-pyridin-3-yl)indazole (184 mg, 0.63 mmol), sodium iodide (189 mg, 1.26 mmol), copper(I)iodide (6 mg, 0.03 mmol) and (lR,2R)-(N,N'-dimethylcyclohexane- 1,2-diamine (8.5 mg, 0.06 mmol) in dioxane (1 ml) were mixed and stirred at 1100C in0 argon. Ammonia (5 ml, 28% in water) and water (20 ml) were added and the mixture was extracted with 2 x 15 ml DCM. The organic phase was concentrated and the crude product was purified by flash chromatography (EtO Ac/heptane) to give the title compound (191 mg, 89 %). APCI-MS m/z: 340 [MH+]. 5
Example 5
N-[(lR,2S)-l-[l-(4-fluorophenyl)indazol-5-yl]oxy-l-phenyl-propan-2- yljmethanesulfonamide
Figure imgf000034_0001
Prepared and purified using the procedure described in Example 2. Yield 11 mg. APCI-MS m/z: 440 [MH+]
Example 6
N-[(lR,2S)-l-[l-(4-fluorophenyl)indazol-5-yl]oxy-l-phenyl-propan-2-yl]-l -phenyl- methanesulfonamide
Figure imgf000034_0002
Prepared and purified using the procedure described in Example 2. Yield 5.7 mg. APCI-MS m/z: 516 [MH+]
Example 7 l,l,l-triβuoro-N-[(lR,2S)-l-[l-(4-fluorophenyl)indazol-5-yl]oxy-l-phenyl-propan-2- yljmethanesulfonam ide
Figure imgf000034_0003
Prepared and purified using the procedure described in Example 2. Yield 9.5 mg. APCI-MS m/z: 494 [MH+]
Example 8 5- [(I R, 2S) -2- (dimethylsulfamoylam ino)-l -phenyl-propoxyj- l-(4-fluorophenyl) indazole
Figure imgf000035_0001
Prepared and purified using the procedure described in Example 2. Yield 12.3 mg. APCI-MS m/z: 469 [MH+]
Example 9
N-[(lR,2S)-l-[l-(4-fluorophenyl)indazol-5-yl]oxy-l-phenyl-propan-2-yl]propane-2- sulfonamide
Chiral
Figure imgf000035_0002
Prepared and purified using the procedure described in Example 2. Yield 1.2 mg. APCI-MS m/z: 468 [MH+]
Example 10
2-(l,3-Dioxoisoindol-2-yl)-N-[(lR,2S)-l-[l-(4-βuorophenyl)indazol-5-yl]oxy-l-phenyl- propan-2-yl] ethanesulfonamide
Chiral
Figure imgf000035_0003
Prepared and purified using the procedure described in Example 2. Yield 2.8 mg. APCI-MS m/z: 599 [MH+]
Example 11 N- [(I R, 2S)-I -[I - (4-βuorophenyl) indazol-5-yl]oxy-l-phenyl-propan-2-yl]-3- (4- methoxyphenoxy)propane-l -sulfonamide
Figure imgf000036_0001
Prepared and purified using the procedure described in Example 2. Yield 11.3 mg. APCI-MS m/z: 590 [MH+]
5 Example 12
N-[(lR,2S)-l-[l-(4-fluorophenyl)indazol-5-yl]oxy-l-phenyl-propan-2- yl] ethanesulfonamide Chιral
Figure imgf000036_0002
Prepared and purified using the procedure described in Example 2. Yield 10.6 mg. I0 APCI-MS m/z: 454 [MH+]
Example 13
N- [(I R, 2S)-I -[I - (4 -fluorophenyl) indazol-5-ylJoxy- 1 -phenyl-propan-2-yl]pentane-2- sulfonamide
Figure imgf000036_0003
Prepared and purified using the procedure described in Example 2. Yield 0.4 mg. APCI-MS m/z: 496 [MH+]
Example 14 0 N-[(lR,2S)-l-[l-(4-fluorophenyl)indazol-5-yl]oxy-l-phenyl-propan-2-yl]butane-2- sulfonamide
Figure imgf000037_0001
Prepared and purified using the procedure described in Example 2. Yield 0.9 mg. APCI-MS m/z: 482 [MH+]
Example 15
N-[(lR,2S)-l-[l-(4-fluorophenyl)indazol-5-yl]oxy-l-phenyl-propan-2- yl] ethanesulfonamide
Figure imgf000037_0002
Prepared and purified using the procedure described in Example 2. Yield 1.2 mg. APCI-MS m/z: 482 [MH+]
Example 16
N- [(I R, 2S)-I -[I - (4-fluorophenyl) indazol-5-ylJoxy- 1 -phenyl-propan-2-ylj '-2 -methyl- propane- 1-sulfonam ide
Figure imgf000037_0003
Prepared and purified using the procedure described in Example 2. Yield 7.2 mg. APCI-MS rα/z: 482 [MH+]
Example 17 N- [(I R, 2S)-I -[I - (4-fluorophenyl) indazol-5-ylJoxy- 1 -phenyl-propan-2-ylJpentane-l- sulfonamide Chiral
Figure imgf000038_0001
Prepared and purified using the procedure described in Example 2. Yield 8.8 mg. APCI-MS m/z: 496 [MH+]
Example 18
3,3,3-trifluoro-N-[(lR,2S)-l-[l-(4-fluorophenyl)indazol-5-yl]oxy-l-phenyl-propan-2- yl] propane- 1 -sulfonamide
Figure imgf000038_0002
Prepared and purified using the procedure described in Example 2. Yield 16.4 mg. APCI-MS m/z: 522 [MH+]
Example 19
Methyl 3-[[(lR,2S)-l-[l-(4-fluorophenyl)indazol-5-yl]oxy-l-phenyl-propan-2- yljsulfamoyljpropanoate o, ,o C yHπ,3 Chiral
F
Prepared and purified using the procedure described in Example 2. Yield 8 mg. APCI-MS m/z: 512 [MH+]
Example 20 l-Cyclopentyl-N-[(lR,2S)-l-[l-(4-fluorophenyl)indazol-5-yl]oxy-l-phenyl-propan-2- yljmethanesulfonamide Chιrai
Figure imgf000039_0001
Prepared and purified using the procedure described in Example 2. Yield 9.1 mg. APCI-MS m/z: 508 [MH+]
5 Example 21
N- [(I R, 2S)-I -[I - (4 -fluorophenyl) indazol-5-yl]oxy-l-phenyl-propan-2- yljcyclopentanesulfonam ide
Figure imgf000039_0002
Prepared and purified using the procedure described in Example 2. Yield 0.7 mg. io APCI-MS m/z: 494 [MH+]
Example 22
2, 2, 2-trifluoro-N- [(I R, 2S)-l-[l-(4-fluorophenyl) indazol-5-yl]oxy-l-phenyl-propan-2- yl] ethanesulfonamide Chiral
Figure imgf000039_0003
Prepared and purified using the procedure described in Example 2. Yield 6.7 mg. APCI-MS m/z: 508 [MH+]
Example 23
2o l-Cyclohexyl-N-[(lR,2S)-l-[l-(4-βuorophenyl)indazol-5-yl]oxy-l-phenyl-propan-2- yljmethanesulfonamide
Figure imgf000040_0001
Prepared and purified using the procedure described in Example 2. Yield 5.6 mg. APCI-MS m/z: 522 [MH+]
Example 24
N-[(lR,2S)-l-[l-(4-fluorophenyl)indazol-5-yl]oxy-l-phenyl-propan-2-yl]hexane-l- sulfonamide
Chiral
Figure imgf000040_0002
Prepared and purified using the procedure described in Example 2. Yield 5.3 mg. APCI-MS m/z: 510 [MH+]
Example 25
N-[(lR,2S)-l-[l-(4-fluorophenyl)indazol-5-yl]oxy-l-phenyl-propan-2-yl]pyridine-3- sulfonamide
Figure imgf000040_0003
Prepared and purified using the procedure described in Example 2. Yield 18.6 mg. APCI-MS m/z: 503 [MH+]
Example 26 (R)-N-(2-methyl-l-phenyl-l-(l-p-tolyl-lH-indazol-5-yloxy)propan-2- yl)cyclopropanesulfonamide
Figure imgf000041_0001
The racemic mixture of N-(l-(l-(4-Fluorophenyl)-lH-indazol-5-yloxy)-2-methyl-l- phenylpropan-2-yl)cyclopropanesulfonamide (26-rac, 10 mg) was separated on Thales SFC, Chiralpak IA column( 70% CO2 , 20%MeOH) collecting the first eluating peak. 5 Yield: 4mg (40%)
APCI-MS: m/z 468 [MH+]
Chiral analysis was made using a CHIRALPAK® IB, 150x0.46 mm column, 10% MeOH /
90% CO2 ,3.5 mL/min, UV=254nm: >98%ee, Rt=9.0 min.
io (RS)-N-(2-methyl-l -phenyl- 1 -(I -p-tolyl-1 H-indazol-5-yloxy)propan-2- yl)cyclopropanesulfonamide (26-rac)
Figure imgf000041_0002
Cyclopropanesulfonyl chloride (155μl, 1.52mmol) was added to l-(l-(4-fluorophenyl)-lH- indazol-5-yloxy)-2-methyl-l-phenylpropan-2-amine (26a, 0.07Og, 0.19 mmol) I5 triethylamine (80μl, 0.57mmol) in MeCN (3 ml) at room temperature. The reaction mixture was stirred over night, concentrated, diluted with 10% NaHSO4 (aq) and extracted with EtOAc. The organic phase was washed with 10% NaHSO4 (aq). The crude product was further purified by HPLC. Yield 25 mg (30%).
1H NMR (400 MHz, DMSO-d6) δ 8.15 (s, IH), 7.73 (dd, J= 9.0, 4.8 Hz, 2H), 7.67 (d, J=0 9.2 Hz, IH), 7.48 (d, J= 7.3 Hz, 2H), 7.37 (m, 4H), 7.25 (m, 2H), 7.05 (d, J= 2.3 Hz, IH),
7.00 (s, IH), 5.46 (s, IH), 2.56 (m, IH), 1.46 (s, 3H), 1.27 (s, 3H), 0.89 (m, 4H).
APCI-MS: m/z 480.2 [MH+]
l-(l-(4-Fluorophenyl)-lH-indazol-5-yloxy)-2-methyl-l-phenylpropan-2-amine (26a)
Figure imgf000042_0001
The subtitle compound was prepared essentially by the metod described by Job &
Buchwald: Org. Lett. 2002, 4 (21), 3703-3706.
A mixture of 2-amino-2-methyl-l-phenylpropan-l-ol (26b, 231 mg, 1.39 mmol), l-(4- Fluorophenyl)-5-iodo-lH-indazole (47 mg, 1.39 mmol), copper(I) iodide (38.1 mg, 0.20 mmol) and Cs2CO3 (1.3 g, 4.20 mmol) in butyronitrile (20 mL) was heated for 5 hours at 1000C in a sealed vial flushed with Argon. The reaction mixture was cooled down, partitioned between EtOAc (20 mL) and water (5 mL), the organic phase was washed with brine . The crude product was purified on ΗPLC Yield 70 mg (14%). APCI-MS: m/z 376.2 [MH+]
2-Amino-2-methyl-l-phenylpropan-l-ol (26b)
Figure imgf000042_0002
37% Hydrochloric acid (1 mL) was added to a stirred solution of 2-methyl-2-nitro-l- phenylpropan-1-ol (26c, 0.12g, 0.6mmol in EtOH (10 mL) and water (5 mL). Zinc powder
(0.241 g, 3.6 mmol) was slowly added in small portions, the mixture was stirred for 4 hours at +700C. The mixture was filtered to remove solid zinc residues and concentrated to
1/3 volume by evaporation, diluted with water (50 mL) and washed with ether (125 mL).
The acidic waterphase was made basic using KOH (aq) solution, the formed slurry was extracted with ether (3x150 mL). The organic phase was dried (MgSO4) filtered and evaporated. The crude product was further purified by HPLC. Yield 60mg (60%).
1H NMR (400 MHz, DMSO-d6) δ 7.27 (m, 5H), 6.14 (s, IH), 5.47 (d, J = 4.8 Hz, IH),
4.90 (s, IH), 1.26 (s, 3H), 1.00 (s, 3H).
APCI-MS: m/z 164 [MH+]
2-Methyl-2-nitro- 1 -phenylpropan- l-ol (26c) To a round bottom flask was added anhyrous magnesium sulphate (3.5g, 29mmol) And 2-nitropropane (16ml). The flask was evacuated and filled with argon. The reaction mixture was stirred vigorously to get a homogeneous suspension before benzaldehyde (1.3ml, 13.1mmol) was added. After stirring in 5 min 2,5,8,9-tetraaza-l- phosphabicyclo[3.3.3]undecane,2,8,9-tris(l-methylethyl) (395mg, 1.3mmol) was added. The reaction mixture was stirred overnight at room temperature before it was purified by flash chromatography (SiO2, heptane-ethylacetate). Yield 0.8g (30%). 1H NMR (300 MHz, DMSO-d6) δ 7.35 (m, 5H), 6.07 (d, J= 4.5 Hz, IH), 5.08 (d, J= 4.4 Hz, IH), 1.39 (s, 3H), 1.33 (s, 3H). APCI-MS: m/z 376.2 [MH+]
Example 27
N-[(lRS,2SR)-l-[l-(4-fluorophenyl)indazol-5-yl]oxy-l-(4-methylsulfanylphenyl)propan-2- yl] cyclopropanesulfonamide
Figure imgf000043_0001
a = relative mixture
The racemic mixture of (li?S,2S70-l-{[l-(4-fiuorophenyl)-lH-indazol-5-yl]oxy}-l-[4- (methylthio)phenyl]propan-2-amine (27a, 11 mg, 0.02 mmol) was dissolved in TΗF (1.5 mL). Excess N-ethyldiisopropylamine (0.185 mL, 1.1 mmol) and cyclopropanesulfonyl chloride (0.063 mL, 0.62 mmol) was added in portions over a period of 3 hours. The reaction mixture was stirred over night at room temperature, quenched by addition of water and purified by ΗPLC. Fractions with product was freezedried to give the title compound as a colourless solid. Chiral ΗPLC was made using an Chiralpak IB™ , 150x0.46 mm column, 15% EtOH in iso-Ηexane, 0.5 mL/min, UV=254nm. two peaks was seen in 1 : 1 ratio. Yield 6 mg (58%)
Chiral ΗPLC : two peaks, 1:1 ratio, Rt = 30.64 + 32.92 min. 1H-NMR (300 MHz, DMSO-d6): δ 8.16 (d, IH), 7.78-7.66 (m, 3H), 7.44-7.30 (m, 5H), 7.28-7.19 (m, 3H), 7.11 (d, IH), 5.28 (d, J=4.38Hz, IH), 3.71 (m, IH), 2.44 (s, 3H), 2.42 (m, IH), 1.24 (d, J=6.76Hz, 3H), 0.90-0.80 (m, 4H) APCI-MS m/z: 512.1 [MH+].
(lRS,2SR)-l-{[l-(4-fluorophenyl)-lH-indazol-5-yl]oxy}-l-[4-(methylthio)phenyl]propan- 2-amine (27a)
Figure imgf000044_0001
The racemic subtitle compound was prepared as described in Exampel Ia o Starting from racemic (li?5',2iS7?)-2-amino-l-[4-(methyltb.io)plienyl]propan-l-ol (49 mg 0.25 mmol), l-(Fluorophenyl)-5-iodoindazole (27b-erythro, 100 mg, 0.3 mmol), CuI (5 mg, 0.03 mmol), Cs2CO3 (163 mg, 0.5 mmol) in butyronitrile (0.5 mL) at +125°C over night. After work up and purification by HPLC the subtitle compound was isolated as the trifluoroacetic acid salt, no NMR was run on this material, LC/MS was used fors identification and all obtained material was used directly in next step. Yield 11 mg (8 %) APCI-MS m/z: 408.1 [MH+ - TFA]
(lRS,2SR)-2-amino-l-[4-(methylthio)phenyl]propan-l-ol & (lR*,2R*)-2-amino-l-[4- (methylthio)phenyl]propan-l-ol (27b-erythro)
Figure imgf000044_0002
Q a = relative mixture
37% Hydrochloric acid (13 mL, 166 mmol) was added to a stirred solution of l-[4- (methylthio)phenyl]-2-nitropropane-l-ol (2.14 g, 9.41 mmol) in EtOH (60 mL) and water (30 mL). Zinc powder (3.7 g, 56.6 mmol) was slowly added in small portions, the mixture was stirred for 4 hours at +700C. The mixture was filtered to remove solid zinc residues and concentrated to 1/3 volume by evaporation, diluted with water (50 mL) and washed with ether (125 mL). The acidic waterphase was made basic using KOH (aq) solution, the formed slurry was extracted with ether (3x150 mL). The organic phase was dried (MgSO4) filtered and evaporated. The crude product was further purified and separated into its two racemic diastereomeric pairs by HPLC.
A small sample of each pair was dissolved in DCM/THF and treated with 1 mol eq 1-1 '- carbonyldiimidazole and cyclizised to the corresponding oxazolidinone that was isolated and analysed by NMR. By comparing shifts and coupling constants with litterature values of oxazolidinones prepared from norephedrines with known stereochemistry {Tetrahedron assym, 1993, vol 4. no 12, pp 2513-2516 & Org. lett, 2005, 7, 13, 2755-2758), the relative sterochemistry of the obtained racemates was determined. Purity analysis of the subtitle compound was made using an Xterra® Cl 8, 5um, 3.0xl00mm column, 20 min gradient of 10% MeCN in 15mM NH3/water to 100% MeCN, 1 mL/min, UV=254nm.
Obtained racemic subtitle compound 27b-erythro:
Yield 243 mg (13%)
HPLC : Rt=3.9 min. 99.3% d.e.
APCI-MS m/z: 198.2 [MH+]. 1H-NMR(SOO MHz, DMSO-d6): δ 7.22 (m, 4H), 5.14 (vbrs, IH, -OH), 4.28 (d, J=4.78Hz, IH), 3.26 (vbrs, 3.3H -NH2+water), 2.86 (brm, IH), 2.45 (s, 3H), 0.84 (d, J=6.37Hz, IH) The above material cyclizised to corresponding racemic oxazolidinone:
(4R*,5S*)-4-methyl-5-[4-(methylthio)phenyl]-l,3-oxazolidin-2-one.
Figure imgf000045_0001
HPLC : Rt=6.27 min. 100% d.e. APCI-MS m/z: 223.9 [MH+]. Η-NMR(300 MHZ, CDCl3): δ 7.25 (m, 4H), 5.68 (d, J=7.83Hz, IH), 5.45 (brs, IH), 4.19 (m, IH), 2.51 (s, 3H), 0.83 (d, J=6.63Hz, 3H).
(lRS,2RS)-2-amino-l-[4-(methylthio)phenyl]propan-l-ol (27b-threo)
Figure imgf000046_0001
a = relative mixture
Isolated as the secondly eluated isomer by the separation of the diastereomers of 2-amino- l-[4-(methylthio)phenyl]propan-l-ol as decribed for 27b-erythro. Yield 418 mg (22%) HPLC : Rt=4.5 min. 96.4% d.e. APCI-MS m/z: 198.2 [MH+]. 1H-NMR(SOO MHz, DMSO-d6): δ 7.21 (m, 4H), 5.22 (vbrs, IH, -OH), 4.07 (brd, IH), 2.75 (brm, IH), 2.45 (s, 3H), 1.51 (vbrs, 2H, -NH2), 0.76 (brd, 3H) The above material cyclizised to corresponding racemic oxazolidinone:
(4R*,5R*)-4-methyl-5-[4-(methylthio)phenyl]-l,3-oxazolidin-2-one.
Figure imgf000046_0002
HPLC : Rt=6.41 min. 100% d.e. APCI-MS m/z: 224.0 [MH+].
Η-NMR(300 MHZ, CDCl3): δ 7.29 (m, 4H), 5.59 (brs, IH), 5.01 (d, J=7.43Hz, IH), 3.82 (m, IH), 2.50 (s, 3H), 1.38 (d, J=6.11Hz, 3H)
l-[4-(methylthio)phenyl]-2-nitropropane-l-ol (27c)
Figure imgf000047_0001
2,8,9-Triisopropyl-2,5,8,9-tetraaza-l-phosphabicyclo-[3.3.3]undecane (395 mg, 1.3 mmol) in Nitroethane (8 mL) was added to a stirred suspension of waterfree MgSO4 (3.5 g) and 4- (methylthio)bensaldehyde (2g, 13.1 mmol) in Nitroethane (8 mL) at room temperature. The yellow slurry was stirred over night, diluted with ether and filtered through a short silica plugg that was washed with ether. Solvents was removed by evaporation and the crude product was purified by flash chromatography using a gradient of 0% EtOAc to 30% EtOAc in Heptane. The product was obtained as an oil that crystallised upon standing. LC/MS and GC/MS failed to give any m/z corresponding to desired mass. NMR supported structure and showed a diastereomeric mixture of 10:19. Yield 2.4 g (81%) 1H-NMRPOO MHz, CDCl3): δ 7.29 (m, 4H), 5.36 (d, J=3.74 Hz, 0.35H), 5.00 (d, J=9 Hz, 0.65H), 4.81-4.63 (m, IH), 2.51+2.50 (s+s, total 3H), 2.39 (vbrs, IH), 1.52 (d, J=6.84 Hz, 1.02H), 1.33 (d, J=6.84 Hz, 1.98H)
Example 28
N- [(I RS, 2RS) -1-fl- (4 -fluorophenyl) indazol-5-yl]oxy-l-(4-methylsulfanylphenyl)propan-2- yljcyclopropanesulfonamide
Figure imgf000047_0002
The racemic mixture of (li?5',2i?1S)-l-{[l-(4-fluorophenyl)-lH-indazol-5-yl]oxy}-l-[4- (methylthio)phenyl]propan-2-amine (28a, 32 mg, 0.06 mmol) was dissolved in TΗF (2 mL). Excess N-ethyldiisopropylamine (0.285 mL, 1.7 mmol) and cyclopropanesulfonyl chloride (0.069 mL, 0.68 mmol) was added in portions over a period of 3 hours. The reaction mixture was stirred over night at room temperature, quenched by addition of water and purified by ΗPLC. Fractions with product was freezedried to give the title compound as a colourless solid. Chiral HPLC was made using an Chiralpak IB , 150x0.46 mm column, 15% EtOH in iso-Hexane, 0.5 mL/min, UV=254nm. two peaks was seen in 1:1 ratio. Yield 9 mg (29%)
Chiral HPLC : two peaks, 1 :1 ratio, Rt = 30.07 + 35.59 min. 1H-NMR (300 MHz, DMSO-(I6): δ 8.16 (d, IH), 7.78-7.64 (m, 3H), 7.44-7.34 (m, 4H), 7.30-7.13 (m, 5H), 5.25 (d, J=5.57Hz, IH), 3.80 (m, IH), 2.48 (m, IH), 2.43 (s, 3H), 1.14 (d, J=6.77Hz, 3H), 0.90-0.81 (m, 4H). APCI-MS mix: 512.1 [MH+].
(lRS,2RS)-l-{[l-(4-fluorophenyl)-lH-indazol-5-yl]oxy}-l-[4-(methylthio)phenyl]propan- 2-amine (28a)
Figure imgf000048_0001
The racemic subtitle compound was prepared as described in Exampel 1.
Starting from racemic (li?iS,2i?1S)-2-amino-l-[4-(methylthio)phenyl]propan-l-ol (27b- threo, 46 mg 0.23 mmol), l-(Fluorophenyl)-5-iodoindazole (95 mg, 0.28 mmol), CuI (5 mg, 0.03 mmol), Cs2CO3 (163 mg, 0.5 mmol) in butyronitrile (0.5 mL) at +125°C over night. After work up and purification by HPLC the subtitle compound was isolated as the Trifluoroacetic acid salt, no NMR was run on this material, LC/MS was used for identification and all obtained material was used directly in next step. Yield 32 mg (26 %) APCI-MS mix: 408.2 [MH+ - TFA]
Example 29
N-[(lR,2S)-l-[l-(4-βuorophenyl)indazol-5-yl]oxy-l-(4-methylsulfanylphenyl)propan-2- yljcyclopropanesulfonamide.
Figure imgf000049_0001
(IR,2S)- 1 - { [ 1 -(4-fluorophenyl)- lH-indazol-5-yl]oxy } - 1 -[4-(methylthio)phenyl]propan-2- amine (29a, 100 mg, 0.24 mmol) was dissolved in MeCN (3 mL), triethylamine (0.104 niL, 0.75 mmol) was added followed by addition of cyclopropane sulfonylchloride (0.025 s mL, 0.25 mmol). The reaction was followed by LC/MS. After 1 hour approximately 50% conversion was detected, more reagents cyclopropane sulfonylchloride (0.025 mL, 0.25 mmol) and triethylamine (0.07 mL, 0.50 mmol) were added in an attempt to increase conversion without any success. The reaction was quenched by addition of saturated NH4Cl (aq) and concentrated. The residual material was purified by HPLC, collectedo fractions was freeze dried to give the title compound as a colourless solid. Chiral HPLC was made using an Chiralpak IB , 150x0.46 mm column, 15% EtOH in iso-Hexane, 0.5 mL/min, UV=254nm, one major peak at 32.39 min (99.7%) and one minor peak at 30.69 min (0.3%). Yield 48 mg (39%). s Chiral HPLC : Rt = 32.39 min, 99.4% e.e.
1H-NMR (300 MHz, DMSO-Cl6): δ 8.16 (d, IH), 7.79-7.66 (m, 3H), 7.45-7.30 (m, 5H), 7.28-7.18 (m, 3H), 7.11 (d, IH), 5.28 (d, J=4.34Hz, IH), 3.71 (m, IH), 2.44 (s, 3H), 2.41 (m, IH), 1.24 (d, 3H), 0.89-0.81 (m, 4H) APCI-MS m/z: 512.2 [MH+]. 0
(lR,2S)-l-{[l-(4-fluorophenyl)-lH-indazol-5-yl]oxy}-l-[4-(methylthio)phenyl]propan-2- amine hydrochloride (29a)
Figure imgf000049_0002
The subtitle compound was prepared as described in Exampel 1. (lR,2S)-2-amino-l-[4-(methylthio)phenyl]propan-l-ol (595mg, 3 mmol), 1- (Fluorophenyl)-5-iodoindazole (913 mg, 2.7 mmol), CuI (28 mg, 0.15 mmol), Cs2CO3 (1.95 g, 6 mmol) in butyronitrile (5 mL) and Toluene (2mL) at +125°C for 6 hours. After work up and purification by HPLC the subtitle compound was isolated as the hydrochloride salt by addition of 6-7 N HCl / 2-Propanol solution and repeated evaporations from MeCN to get the subtitle compound as a beige solid. Yield 300 mg
(25%).
1H-NMR (300 MHz, DMSO-d6): δ 8.40 (brs, 3H), 8.20 (d, IH), 7.79-7.71 (m, 3H), 7.45-
7.24 (m, 7H), 7.14 (d, IH), 5.69 (d, J=2.92 Hz, IH), 3.65 (m, IH), 2.45 (s, 3H), 1.19 (d, 3H).
APCI-MS m/z: 408.0 [MH+-HCl]
(lR,2S)-2-amino-l-[4-(methylthio)phenyl]propan-l-ol hydrochloride (29b)
Figure imgf000050_0001
The subtitle compound was prepared following the procedure described by Jingjun Yin et. al. J. Org. Chem. 2006, 71, 840-843.
A mixture of (^-tert-butyl l-(4-(methylthio)phenyl)-l-oxopropan-2-ylcarbamate (29c, 2.7 g, 9.14 mmol), Aluminium isopropoxide (0.373g, 1.83 mmol) and 2-propanol (7.75 mL, 100.54 mmol) in toluene (11.5 mL) was heated at +50 °C under argon for 16 hours. The reaction mixture was allowed to cool, EtOAc (100 mL) and 0.5N HCl (60 mL) was added, the organic layer was washed with water and brine, dried over Na2SO4, filtered and evaporated to give 2.65 crude product as a colourless solid.
The crude product was further purified by flash chromatography on silica, a gradient of 10% EtOAc to 30 % EtOAc in Heptane followed by isocratic final concentration until all product had been eluted. Obtained 2.18 g of the intermediate BOC-protected subtitle compound as a colourless solid. APCI-MS m/z: 180.1, 198.1, 224.1 [MH+-BOC-water, MH+-BOC, MH+-tBu- water]. The obtained material was dissolved in EtOAc (50 mL) and treated with 1.5 M HCl in EtOAc (40 ml, 60.00 mmol) at +70 °C for 90 minutes. Solvents was removed by evaporation and the solid residue was suspended in EtOAc (30 mL) and Et2O (100 mL), the salt was collected by filtration and washed with ether (50 mL). Yield 1.68 g (78% yield) APCI-MS m/z: 198.1 [MH+] 1H-NMR (400 Mhz, DMSO-d6) : δ 8.05 (brs, 3H), 7.30 (d, 2H), 7.26 (d, 2H), 6.02 (d, J=4.24Hz, IH), 4.89 (t, IH), 3.35 (m, IH), 2.47 (s, 3H), 0.94 (d, 3H)
A sample of the above material was cyclizised to the corresponding oxazolidinone with 1 mol eq of 1-1 '-carbonyldiimidazole and triethylamine in DCM. By NMR-analysis and comparing shifts and coupling constants with litterature values of oxazolidinones prepared from norephedrines with known stereochemistry (Tetrahedron assym, 1993, vol 4. no 12, pp 2513-2516 & Org. lett, 2005, 7, 13, 2755-2758), the relative sterochemistry, and consequently the absolute stereochemistry of the subtitle compound was determined. (4S,5R)-4-methyl-5-[4-(methylthio)phenyl]-l,3-oxazolidin-2-one.
Figure imgf000051_0001
APCI-MS m/z: 224.0 [MH+]
1H-NMR (500 MHz, CDCl3): δ 7.27 (d, 2H, Ar-H), 7.22 (d, 2Η), 5.77 (brs, IH, -NH), 5.68 (d, JK7.96 Hz, IH, PhCH-), 4.19 (m, 1Η, -CH(Me)-), 2.50 (s, 3Η, Ar-SCH3), 0.83 (d, J=6.5 Hz, 3H, -CH3) ppm.
(S)-tert-butyl l-(4-(methylthio)phenyl)-l-oxopropan-2-ylcarbamate (29c)
Figure imgf000051_0002
(S)-tert-buty\ l-(methoxy(methyl)amino)-l-oxopropan-2-ylcarbamate (2.32 g, 9.99 mmol) was suspended in dry TΗF (20 mL) and inerted with Argon. The slurry was cooled using an ice/acetone bath to -15 to -1O0C and slowly charged with isopropylmagnesium chloride 2.0M solution in THF (4.74 mL, 9.49 mmol). After addition a clear solution was obtained, to this solution was slowly added 4-thioanisolemagnesium bromide 0.5M in THF (24 mL, 12.00 mmol), after addition the rection mixture was stirred at room temperature for 4 hours. The reaction was quenched by pouring it into 1 N HCl (10OmL), EtOAc (250 mL) was added to the mixture. The phases was separated, the organic phase was washed with brine, and the waterphases were back extracted once with EtOAc. The combined EtOAc phases were dried (Na2SO4), filtered and evaporated. The crude product was purified by flash chromatography on silica. A solvent gradient was used. 100% Heptane to 50%Heptane/DCM + 5%MeOH, and then kept at final solvent ratio until product was eluted. Fractions with product was combined and solvent evaporated to give the subtitle compound as a colourless solid. Yield 2.7 g (92%) LC/MS (APCI) : (M+l)=295.9 1H-NMR (300 MHz, DMSO-(I6): δ 7.89 (d, 2H), 7.36 (d, 2H), 7.28 (d, IH), 5.00 (m, IH), 2.54 (s, 3H), 1.35 (s, 9H), 1.21 (d, 3H)
APCI-MS m/z: 295.9, 195.9 [MH+, MH+-BOC]
Example 30
N- [(I R, 2S)- 1 -phenyl- 1- (1 -propan-2-ylindazol-5-yl)oxy-propan-2-yl]methanesulfonam ide
Figure imgf000052_0001
The title compound was prepared from (li?,2ιS)-l-[(l-isopropyl-lH-mdazol-5-yl)oxy]-l- phenylpropan-2-amine (30b, 31 mg, 100 μmol) and methanesulfonyl chloride (34 mg, 300 μmol) as described in Example 2b. Yield 28 mg (72 %). APCI-MS: m/z 388 [MH+] 1H NMR (400 MHz, ^-acetone) δ 7.75 (s, IH), 7.50 (m, 3H), 7.38 (t, J= 7.5 Hz, 2H), 7.29 (m, IH), 7.15 (dd, J= 9.0, 2.3 Hz, IH), 7.03 (d, J= 2.3 Hz, IH), 6.26 (d, J= 8.8 Hz, IH), 5.41 (d, J= 4.4 Hz, IH), 4.88 (septet, J= 6.7 Hz, IH), 3.90 (m, IH), 2.77 (s, 3H), 1.47 (dd, J= 6.7, 3.5 Hz, 6H), 1.32 (d, J= 6.7 Hz, 3H). (lR,2S)-l-[(l-isopropyl-lH-indazol-5-yl)oxy]-l-phenylpropan-2-amine (30b)
Figure imgf000053_0001
A mixture of 5-iodo-l-isopropyl-lH-indazole (30c, 461 mg, 1.26 mmol), (li?,2.S)-2-amino- 1-phenylpropan-l-ol (286 mg, 1.89 mmol), copper (I) iodide (25 mg, 130 μmol), and 5 cesium carbonate (1.45 g, 3.8 mmol) in butyronitrile (5 ml) was stirred at 125 °C for 2 h. Then the mixture was cooled to room temp., the inorganic material was removed by filtration and washed with ethyl acetete. The combined organic solutions were concentrated i. vac, and the product purified by flash chromatography on silica gel (ethyl acetate/methanol). Yield 200 mg (51 %) of a brown oil. io APCI-MS: m/z 310 [MH+]
1H NMR (400 MHz, DMSO-^/D2O/TFA) δ 7.80 (s, IH), 7.53 (d, J= 9.0 Hz, IH), 7.40 (d, J= 7.1 Hz, 2H), 7.33 (t, J = 7.5 Hz, 2H), 7.24 (m, IH), 7.08 (dd, J= 9.0, 2.3 Hz, IH), 6.98 (d, J= 2.1 Hz, IH), 5.75 (s, IH), 5.03 (d, J= 5.3 Hz, IH), 4.86 (septet, J= 6.7 Hz, IH), 3.15 (quintet, J= 6.0 Hz, IH), 1.41 (dd, J= 6.4, 5.5 Hz, 6H), 1.06 (d, J= 6.5 Hz, 3H).
I5
5-Iodo-l -isopropyl-1 H-indazole (30c)
Figure imgf000053_0002
A mixture of 5-iodo-lH-indazole (488 mg, 2 mmol), isopropyl bromide (244 mg, 2 mmol), and KO/Bu (336 mg, 3 mmol) in dry DMF (4 ml) was stirred at room temp, overnight.0 Then it was dilited with ethyl acetate (50 ml), washed with water (2 x 50 ml), and dried with Na2SO4. Evaporation of solvent and purification by flash chromatography on silica gel («-heptane/ethyl acetate) afforded the subtitle compound (298 mg, 52 %) along with 5- iodo-2-isopropyl-2H-indazole (227 mg, 40 %).
1H NMR (400 MHz, CDCl3) δ 8.11 (d, J= 0.9 Hz, IH), 7.94 (s, IH), 7.60 (dd, J= 8.8, 1.55 Hz, IH), 7.26 (d, J= 8.8 Hz, IH), 4.83 (septet, J= 6.8 Hz, IH), 1.61 (d, J= 6.7 Hz, 6H).
APCI-MS: m/z 287 [MH+] Example 31
N-[(lR,2S)-l-[l-(4-fluorophenyl)indazol-5-yl]oxy-l-(4-methylsulfinylphenyl)propan-2- yl] cyclopropanesulfonamide
Figure imgf000054_0001
N-[( IR,2S)- 1 -[ 1 -(4-fluorophenyl)indazol-5-yl] oxy- 1 -(4-methylsulfanylphenyl)propan-2- yl]cyclopropanesulfonamide (29, 20.5 mg, 0.04 mmol) was dissolved in DCM (2 mL) and cooled in an icebath. 3-Chloroperbenzoic acid (70-75%) (10 mg, 0.04mmol) dissolved in DCM (0.1 mL) was added. The reaction was stirred for 30 min, quenched by addition of 10% Na2SO3 solution (0.5 mL). Purification by HPLC and freezedrying of fractions with product gave the title compound as a colourless solid. Yield 17 mg (80%).
1H-NMR (300 MHz, DMSO-(I6): δ 8.17 (d, IH), 7.78-7.58 (m ,7H), 7.48-7.35 (m, 3H), 7.25 (dd, IH), 7.16 (d, IH), 5.40 (d, J=4.51Hz, IH), 3.77 (m, IH), 2.72 (s, 3H), 2.42 (m, IH), 1.25 (d, 3H), 0.90-0.80 (m, 4H) APCI-MS m/z: 528.1 [MH+]
Example 32
N-[(lR,2S)-l-(l-cyclopentylindazol-5-yl)oxy-l-phenyl-propan-2- yl] cyclopropanesulfonamide
Chiral
Figure imgf000054_0002
The title compound was prepared from (^^^-^[(l-cyclopentyl-lH-indazol-S-y^oxyJ-l- phenylpropan-2-amine (32b, 17 mg, 51 μmol) and cyclopropanesulfonyl chloride (34 mg, 153 μmol) as described in Example 2b. Yield 10 mg (45 %).
1H NMR (400 MHz, ^-acetone) δ 7.73 (s, IH), 7.49 (m, 3H), 7.37 (t, J= 7.5 Hz, 2H), 7.28 (m, IH), 7.16 (dd, J= 9.0, 2.3 Hz, IH), 7.02 (d, J= 2.3 Hz, IH), 6.30 (d, J= 9.0 Hz, IH), 5.47 (d, J= 3.9 Hz, IH), 5.06 (quintet, J= 7.1 Hz, IH), 3.90 (m, IH), 2.42 (m IH), 1.88 (m, 2H), 1.70 (m, 2H), 1.33 (d, J= 6.7 Hz, 3H), 1.01 - 0.83 (m, 4H). APCI-MS: m/z 440 [MH+]
(lR,2S)-l-[(l-cyclopentyl-lH-indazol-5-yl)oxy]-l-phenylpropan-2-amine (32b)
Figure imgf000055_0001
Ppereped froml-cyclopentyl-S-iodo-lH-indazole (37c, 158 mg, 500 μmol) as described for 30b. YiId 34 mg (20 %). APCI-MS: m/z 336 [MH+]
l-Cyclopentyl-5-iodo-lH-indazole (32c)
Figure imgf000055_0002
A mixture of 2-fluoro-5-iodobenzaldehyde (500 mg, 2 mmol), cyclopentylhydrazine (273 mg, 2 mmol), and cesium carbonate (1.91 g, 5 mmol) in NMP (5ml) was stirred at 100 0C overnight. Then KOfBu (560 mg, 5 mmol) and DMF (10 ml) were added, and the mixture was stirred at 150 0C for 5 h. After cooling to room temp., the mixture was diluted with ethyl acetate (100 ml), and washed with water (3 x 50 ml), and dried. Eveporation of solvent afforded balch residue, which was dissolved in acetonitrile (50 ml), and the insoluble material was removed vy filtration. Flash chromatography on silica gel («- heptane/ethyl acetate) afforded yellow oil, 158 mg (25 %). 1H NMR (400 MHz, CDCl3) δ 8.08 (d, J= 0.9 Hz, IH), 7.91 (s, IH), 7.59 (dd, J= 8.8, 1.5 Hz, 1 H), 7.26 (d, J = 9.4 Hz, 1 H, partially covered tiwh the signal of solvent), 4.95 (quintet, J= 7.4 Hz, IH), 2.17 (m, 4H), 1.98 (m, 2H), 1.75 (m, 2H). APCI-MS: m/z 313 [MH+]
Example 33 N-[(lR,2S)-l-phenyl-l-(l-propan-2-ylindazol-5-yl)oxy-propan-2- yljcyclopropanesulfonamide
Figure imgf000056_0001
The title compound was prepared from (li^^-l-Kl-isopropyl-lH-indazol-S-ytyoxy]-!- phenylpropan-2-amine (30b, 31 mg, 100 μmol) and cyclopropanelsulfonyl chloride (42 mg, 300 μmol) as described in Example 2b. Yield 33 mg (80 %). s 1H NMR (400 MHz, Js-acetone) δ 7.74 (s, IH), 7.49 (m, 3H), 7.38 (t, J= 7.5 Hz, 2H), 7.28 (m, IH), 7.16 (dd, J= 9.2, 2.3 Hz, IH), 7.03 (d, J= 2.3 Hz, IH), 6.30 (d, J= 8.8 Hz, IH), 5.47 (d, J= 4.1 Hz, IH), 4.88 (septet, J= 6.7 Hz, IH), 3.90 (m, IH), 2.42 (m, IH), 1.48 (dd, J= 6.6, 3.8 Hz, 6H), 1.33 (d, J= 6.9 Hz, 3H), 1.01-0.83 (m, 4H). APCI-MS: m/z 414 [MH+] 0
Example 34
N-[(lR,2S)-l-[l-(4-βuorophenyl)indazol-5-yl]oxy-l-(4-methylsulfonylphenyl)propan-2- yl] cyclopropanesulfonamide.
Figure imgf000056_0002
s N-[(IR,2S)- 1 -[ 1 -(4-fluorophenyl)indazol-5-yl]oxy- 1 -(4-methylsulfanylphenyl)propan-2- yl] cyclopropanesulfonamide (29, 19 mg, 0.037 mmol) was dissolved in acetic acid (1.5 mL), 35% H2O2 (1 mL) was added and the mixture was stirred at +60°C for 45 minutes. After cooling to room temperature the reaction mixture was diluted with water and purified by HPLC. The collected fractions containing the desired product was freezedried. Obtained the title compound as a colourless solid. Yield 16 mg (57%).
1H-NMR (300 MHz, DMSO-(I6): δ 8.17 (d, IH), 7.94 (d, 2H), 7.78-7.65 (m, 5H), 7.49-7.35 (m, 3H), 7.26 (dd, IH), 7.16 (d, IH), 5.45 (d, j=4.51 Hz, IH), 3.80 (m, IH), 3.20 (s, 3H), 2.45 (m, IH), 1.25 (d, 3H), 0.90-0.80 (m, 4H) APCI-MS m/z: 544.1 [MH+] Example 35
N-[(lRS,2SR)-l-[6-chloro-l-(4-βuorophenyl)indazol-5-yl]oxy-l-(4-fluorophenyl)propan- 2-yl]cyclopropanesulfonamide
Figure imgf000057_0001
Prepared according to the procedure described for Example 1 starting with (I RS, 2SR)-I -[6- chloro- 1 -(4-fluorophenyl)indazol-5-yl]oxy- 1 -(4-fluorophenyl)propan-2-amine (35a-rac-2, 55 mg, 0.13 mmol) and cyclopropylsulphonyl chloride (42 μl, 0.4 mmol). Yield: 2 mg
<v O. ).
1H NMR (400 MHz, CD3OD) δ 8.02 (d, J= 0.7 Hz, IH), 7.78 (s, IH), 7.67 (td, J= 8.7, 3.8 Hz, 2H), 7.48 (dd, J= 8.7, 5.3 Hz, 2H), 7.31 (ddd, J= 12.4, 8.5, 3.7 Hz, 2H), 7.19 (s,
IH), 7.11 (t, J= 8.8 Hz, 2H), 5.48 (d, J= 4.6 Hz, IH), 3.90 (dt, J= 11.5, 6.8 Hz, IH), 2.39
(tt, J= 8.0, 4.8 Hz, IH), 1.41 (d, J= 6.9 Hz, 3H), 1.03 - 0.99 (m, 2H), 0.92 - 0.87 (m,
2H).
APCI-MS: 518 m/z [MH+]
l-[6-chloro-l-(4-βuorophenyl)indazol-5-yl]oxy-l-(4-fluorophenyl)propan-2-amine (35a)
Chiral
Figure imgf000057_0002
a = relative mixture
1 - {[6-Chloro- 1 -(4-fluorophenyl)- 1 H-indazol-5-yl]oxy} - 1 -(4-fluorophenyl)acetone (500 mg, 1.21 mmol), ammonium acetate (35b, 934 mg, 12.11) and cyanoborohydride on polymer support (1.82 g, 3.63 mmol) were mixed in methanol (3 ml) and heated in micro at 140°C for 10 min. The mixture was concentrated and treated with NaHCO3 and DCM. The organic phase was concentrated and the crude product was purified by flash chromatography (EtO Ac/heptane followed by EtOAc/methanol). The diasteromers were separated on preparative HPLC (Kromasil column, water buffered with 2g NH4OAc/!, pH set to 5.5 with HOAc, and MeCN, 25%-75%) to give the syn-isomer (WS,2RS)-l-[6- chloro- 1 -(4-fluorophenyl)indazol-5-yl]oxy- 1 -(4-fluorophenyl)propan-2-amine (35a-rac-l) as first eluated isomer (assignment by IH-NMR).
1H NMR (400 MHz, CD3OD) δ 8.05 (d, J= 0.9 Hz, IH), 7.75 (s, IH), 7.65 (tt, J= 4.6, 2.3 5 Hz, 2H), 7.53 (dd, J= 12.0, 1.9 Hz, 2H), 7.34 - 7.26 (m, 3H), 7.13 (t, J= 8.8 Hz, 2H), 5.28 (d, J= 8.3 Hz, IH), 3.69 (dd, J= 8.2, 6.8 Hz, IH), 1.17 (d, J= 6.7 Hz, 3H). APCI-MS: 414 m/z [MH+].
The a«/z-isomer (li?5',25/?)-l-[6-chloro-l-(4-fluorophenyl)indazol-5-yl]oxy-l-(4- io fluorophenyl)propan-2-amine (35a-rac-2) was eluated secondly.
1H NMR (400 MHz, CD3OD) δ 8.02 (s, IH), 7.79 (s, IH), 7.69 - 7.64 (m, 2H), 7.48 - 7.43 (m, 2H), 7.31 (dd, J= 20.8, 3.4 Hz, 2H), 7.18 - 7.10 (m, 3H), 5.41 (d, J= 4.6 Hz, IH), 3.46 (dt, J= 11.1, 6.6 Hz, IH), 1.27 (d, J= 6.5 Hz, 3H). APCI-MS: 414 m/z [MH+].
I5 l-{[6-chloro-l-(4-fluorophenyl)-lH-indazol-5-yl]oxy}-l-(4-βuorophenyl)acetone (35b)
Figure imgf000058_0001
4-Fluorophenylacetone (388 μl, 2.9 mmol) in DCM (12 ml) was cooled to 0°C and bromine (672 mg, 2.9 mmol) was slowly added. The mixture was stirred for 30 min and0 then concentrated in vacuo. The crude intermediate was added to a mixture of 6-chloro-l- (4-fluorophenyl)-lH-indazol-5-ol, preparation described in case 102561, example 4, (762 mg, 2.9 mmol) and potassium carbonate (804 mg, 5.8 mmol) in TΗF (12 ml). The mixture was stirred for 4 h, filtrated and concentrated. The crude product was purified by flash chromatography (EtO Ac/heptane, product eluted at 40% EtOAc) to give the title 5 compound (1.06 g, 88%). APCI-MS: 413 m/z [MH+].
Example 36 N-[(lRS,2RS)-l-[6-chloro-l-(4-βuorophenyl)indazol-5-yl]oxy-l-(4-fluorophenyl)propan- 2-yl]cyclopropanesulfonamide
Figure imgf000059_0001
a = relative mixture
Prepared according to the procedure described for Example 1 starting with (\RS,2SR)-\-[6- chloro-l-(4-fluorophenyl)indazol-5-yl]oxy-l-(4-fluorophenyl)propan-2-amine (35a-rac-l, 35 mg, 0.08 mmol) and cyclopropylsulphonyl chloride (27 μl, 0.25 mmol). Yield: 2 mg (5 %).
1H NMR (400 MHz, CD3OD) δ 8.04 (d, J= 0.7 Hz, IH), 7.77 (s, IH), 7.67 (td, J= 8.7, 4.1 Hz, 2H), 7.53 (dd, J= 8.8, 5.4 Hz, 2H), 7.31 (ddd, J= 15.6, 5.3, 3.3 Hz, 2H), 7.26 (s, IH), 7.10 (t, J= 8.8 Hz, 2H), 5.49 (d, J= 5.1 Hz, IH), 4.04 (dt, J= 12.0, 6.8 Hz, IH), 2.52 (tt, J= 8.0, 4.9 Hz, IH), 1.29 (d, J= 6.9 Hz, 3H), 1.05 - 0.90 (m, 4H). APCI-MS: 518 m/z [MH+]
Example 37 (R)-N- [2- [l-(4-fluorophenyl)indazol-5-yl]sulfanyl-2-phenyl- ethyl] cyclopropanesulfonam ide
Figure imgf000059_0002
The racemic mixture of N-(I -(I -(4-fluorophenyl)-l H-indazol-5-ylthi o)-2 -methyl- 1- phenylpropan-2-yl)cyclopropanesulfonamide (37-rac, 10 mg) were separated on Thales SFC, Chiralpak IA column( 74% CO2 , 26%MeOH) collecting the first eluating peak. Yield: 4mg (40%). APCI-MS: m/z 376.2 [MH+]
Chiral analysis was made using a CHIRALPAK® IB, 150x0.46 mm column, 26% MeOH / 74% CO2 , 3.5 mL/min, UV=254nm: >98%ee, Rt=6.78 min N-(2-(l-(4-fluorophenyl)-lH-indazol-5-ylthio)-2-phenylethyl)cyclopropanesulfonamide f37a)
Figure imgf000060_0001
Cyclopropanesulfonyl chloride (7μl, 0.068mmol was added to 2-phenyl-2-(4-p-tolyl-lH- indazol-5-yloxy)-ethylamine (37b, O.Olg, 0.027mmol), triethylamine (15μl, 0.11 mmol) in MeCN(ImI) at room temperature. The reaction mixture was stirred for 2 hours, concentrated, diluted with 10% NaHSO4 (aq) and extracted with EtOAc. The organic phase was washed with 10% NaHSO4 (aq). The crude product was further purified by HPLC. Yield 12 mg (95%).
1H NMR (400 MHz, DMSO-d6) δ 8.33 (s, IH), 7.92 (s, IH), 7.78 (dd, J= 8.9, 4.8 Hz, IH), 7.73 (d, J= 9.2 Hz, 2H), 7.43 (s, 3H), 7.31 (d, J = 4.2 Hz, 6H), 4.43 (t, J = 22.1 Hz, IH), 3.49 (s, 2H), 2.41 (m, IH), 0.78 (s, 4H). APCI-MS: m/z 468 [MH+]
2-(l-(4-Fluorophenyl)-lH-indazol-5-ylthio)-2-phenyl-ethylamine (37b)
Figure imgf000060_0002
l-(4-Fluorophenyl)-lH-indazole-5-thiol (37c, 0.068 g, 0.28 mmol) , (£)-(2- nitrovinyl)benzene (0.055 g, 0.37 mmol) and DMAP (cat.amount) in THF (5 ml ) were stirred at 7O0C for 2 hours. The solvent was evaporated and the mixture was partitioned between water/EtOAc. The organic phase was dried and evaporate under reduced pressure. To this mixture EtOH(5ml), water (3ml), cone HCl (ImI) and Zn (0.26 g) were added and the mixture was stirred at 7O0C for 2 hours. After cooling to room temperature water (15 ml) was added and the EtOH was evaporated. The pH of the mixture was adjusted to pHIO with NaOH (5N, aq) and extracted with EtOAc (3*25ml). The organic phases were combined and dired, the solvent was evaporated under reduced pressure . Purification was done by HPLC. Yield 0.01Og (10%) APCI-MS: m/z 364 [MH+]
l-(4-Fluorophenyl)-lH-indazole-5-thiol (37c)
Figure imgf000061_0001
To 5'-l-(4-Fluorophenyl)-lH-mdazol-5-yl benzothioate (37d, 0.046 g, 0.13 mmol) in methanol (3 mL) , potassium carbonate (0.011 mL, 0.20 mmol) was added and the mixture was stirred at rt for 2 hrs. water was than added, IN HCl (2 ml) and extracted with EtOAc (2*20ml), dried, evaporated and then purified on HPLC. The relevant fractions were collected freeze dried and analysed by LC/MS. APCI-MS: m/z 245 [MH+]
S-l-(4-fluorophenyl)-lH-indazol-5-yl benzothioate (37d)
Figure imgf000061_0002
To a solution of l-(4-fluorophenyl)-5-iodo-lH-indazole (0.224 g, 0.66 mmol), thiobenzoic acid (0.093 ml, 0.79 mmol), 3,4,7,8-tetramethyl-l,10-phenantroline (0.031 g, 0.13 mmol) and N,N-diisopropylamine (0.220 ml, 1.32 mmol) in toluene (2.5 ml) was added copper(I) iodine (2.245 μl, 0.07 mmol). The resulting mixture was stirred at HOC O/Ν. The reaction mixture was cooled to rt diluted with EtOAc and washed with water. The organic phase was the dried the solvent evaporated and then purified on ΗPLC.The relevant fractions were collected freezdried to give 45mg (20%) of product wich was analysed by LC/MS. APCI-MS: m/z 349 [MH+]
Example 38 N-((lR,2S)-l-(l-(4-fluorophenyl)-lH-indazol-5-ylthio)-l-phenylpropan-2- yl)methanesulfonamide
Figure imgf000062_0001
To (2i?,3£)-2-methyl-l-(methylsulfonyl)-3-phenylaziridine (38a, 0.03 g, 0.14 mmol) in 5 THF (4ml) was added 1 -(4-Fluorophenyl)- 1 H-indazole-5-thiol (37c, 0.038 g, 0.16 mmol) and sodium hydride (5.11 mg, 0.21 mmol) on an ice bath with stirring. The ice bath was removed and the mixture was stirren at roomtemperature over night. The solvent was removed and the mixture was purified on HPLC. Yield 0.007g (11%).
1H NMR (500 MHz, DMSO-d6) δ 8.27 (d, J= 0.7 Hz, IH), 7.79 (d, J= 1.0 Hz, IH), 7.74 I0 (m, 2H), 7.66 (d, J= 8.9 Hz, 2H), 7.40 (m, 5H), 7.29 (t, J= 7.6 Hz, 2H), 7.19 (m, 2H),
4.42 (d, J= 6.3 Hz, IH), 4.14 (t, J= 5.2 Hz, IH), 2.62 (s, 3H), 1.35 (s, 3H).
APCI-MS: m/z 456 [MH+]
(2R, SR)-2-Methyl-l-(methylsulfonyl)-3-phenylaziridine (38a) Chiral
Figure imgf000062_0002
To (2i?,3i?)-2-Methyl-3-phenylaziridine (38b, 0.08 g, 0.60 mmol) in CH2Cl2 (3 ml) was added N,N-Diisopropylmethyamine (0.219 ml, 1.32 mmol) and the reaction mixture was cooled to -1O0C before methansulfonyl chloride (0.051 ml, 0.66 mmol) was added The mixture was stirred for 30 min at -1O0C than at room temperature for 1 h. The crude
20 sample was added to a Chromasil Cl 8 column and was eluted with MeCN/H2O 35-70%, 20min.
Yield 0.055g (43%).
1H NMR (300 MHz, DMSO-d6) δ 7.36 (m, 5H), 3.72 (d, J= 4.4 Hz, IH), 3.13 (s, 3H), 3.03 (m, IH), 1.64 (d, J= 6.0 Hz, 3H). s APCI-MS: m/z 253.1 [MH+] (2R, 3R)-2-Methyl-3-phenylaziridine (38b)
Figure imgf000063_0001
To (liϊ^-O-norephedrine (1 g, 6.61 mmol) in THF (20 mL), triphenylphosphine (1.98 ml, 7.94 mmol), diisopropyl azodicarboxylate (1.401 ml, 7.27 mmol) and triethylamine s (2.6 ml, 18.8 mmol) was added anr the rm was stirred at room temperature over night. The solvent was evaporated and the crude material was purified on silica eluting with Heptane/EtPAc 1+4 to EtOAc 100% and EtOAc/MeOH 95+5% the fractions containg product were collected giving 2.5 g of with solid containg lot OfP(Ph)3O according to LC/MS.This solid was stirred in hexane (50ml) O/N and than filtered through a pad ofo Celite 545 giving 0.6g of an oil.
1H NMR (400 MHz, CDCl3) δ 7.32 (m, 5H), 2.75 (d, J= 2.7 Hz, IH), 2.22 (m, IH), 1.45 (d, J= 5.5 Hz, 3H) APCI-MS m/z 134.1 [MH+] s Example 39
N- [(I R, 2S)-I -[I -(4-fluorophenyl) indazol-5-ylth iojsulfonyl- 1 -phenyl-propan-2- yljmethanesulfonamide
Figure imgf000063_0002
N-(( IR,2S)- 1 -( 1 -(4-Fluorophenyl)- 1 H-indazol-5-ylthio)- 1 -phenylpropan-2- yl)methanesulfonamide (Example 38, 3.00 mg, 6.59 μmol) was dissolved in H2O2 (0.5 ml, 16.32 mmol) and acetic acid (0.5 ml) and stirred at 50°C for 120 min. After removing of the solvents the mixture was purified on HPLC. Yield 0.0015g (46%) 1H NMR (500 MHz, DMSO-d6) δ 8.51 (s, IH), 8.19 (d, J= 1.1 Hz, IH), 7.75 (q, J= 4.6 Hz, 3H), 7.56 (m IH), 7.46 (d, J= 8.8 Hz, 2H), 7.26 (m, 2H), 7.20 (m, 3H), 7.08 (m, IH), 4.58 (m, IH), 4.40 (m, IH), 2.61 (s, 3H), 1.46 (d, J= 6.6 Hz, 3H). APCI-MS: m/z 505 [MH++! 8] Example 40
N- [(2R)-2-[ 1 -(4 -fluorophenyl) indazol- 5 -y I] ' oxy-2-phenyl-ethyl] ' cyclopropanesulfonamide
Figure imgf000064_0001
Prepared according to the procedure described for Example 1 from [(li?)-2-[l-(4- fluorophenyl)indazol-5-yl]oxy-l-phenyl-ethan-2-amine (40a, 31 mg, 0.09 mmol) cyclopropylsulphone chloride (28 μl, 0.27 mmol). Yield: 10 mg (25%). 1H NMR (400 MHz, CD3OD) δ 8.01 (s, IH), 7.68 - 7.63 (m, 2H), 7.58 (d, J= 9.2 Hz, IH), 7.47 (d, J= 7.3 Hz, 2H), 7.37 (t, J= 7.4 Hz, 2H), 7.32 - 7.24 (m, 4H), 7.14 (d, J= 2.3 Hz, IH), 5.41 (dd, J= 8.1, 4.1 Hz, IH), 3.61 - 3.47 (m, 2H), 2.51 (tt, J= 8.0, 4.8 Hz, IH), 1.06 - 0.90 (m, 4H).
APCI-MS: 452 m/z [MH+]
[(lR)-2-[l-(4-fluorophenyl)indazol-5-yl]oxy-l-phenyl-ethan-2-amine (40a) Prepared from (lR)-l-phenyl-ethan-2-amine (294 mg, 2.15 mmol) and l-(fluorophenyl)-5- iodoindazole (484 mg, 1.43 mmol) according to the protocol for Ia. Yield: 125 mg (17 %). APCI-MS: 348 m/z [MH+]
Example 41
N-[(2S)-2-[l-(4-fluorophenyl)indazol-5-yl]oxy-2-phenyl-ethyl] cyclopropanesulfonamide
Figure imgf000064_0002
Prepared according to the procedure described for Example 1 from [(liS)-2-[l-(4- fluorophenyl)indazol-5-yl]oxy-l-phenyl-ethan-2-amine (41a, 31 mg, 0.09 mmol) cyclopropylsulphone chloride (28 μl, 0.27 mmol). Yield: 22 mg (54 %).
[(I S)-2- [ 1 -(4-fluorophenyl)indazol-5-yl] oxy-1 -phenyl-ethan-2-amine (41a) Prepared from (l1S)-l-phenyl-ethan-2-amine (294 mg, 2.15 mmol) and l-(fluorophenyl)-5- iodoindazole (484 mg, 1.43 mmol) according to the protocol for Ia. Yield: 175 mg (23 %). APCI-MS: 348 m/z [MH+]
s Example 42
N-((lR,2S)-l-(l-(4-fluorophenyl)-lH-indazol-5-yloxy)-l-(quinolin-3-yl)propan-2- yl)cyclopropanesulfonamide.
Figure imgf000065_0001
(IR, 2S)- 1 -( 1 -(4-fiuorophenyl)- lH-indazol-5-yloxy)- 1 -(quinolin-3 -yl)propan-2-amineo bis(2,2,2-trifluoroacetate) (42a, 65 mg, 0.10 mmol) was dissolved in TΗF (1.5 mL), triethylamine (75 μl, 0.54 mmol) was added followed by cyclopropanesulfonyl chloride (15 μl, 0.15 mmol). The reaction mixture was stirred at room temperature, after 1.5 hours another portion of triethylamine (75 μl, 0.54 mmol) and excess of cyclopropanesulfonyl chloride (50 μl, 0.49 mmol) was added. The reaction mixture was left over night at rooms temperature. Solvent was removed by evaporation and the residual material was purified by ΗPLC. Yield 18 mg (34%).
1H-NMR (300 MHz, DMSO-d6): δ 8.99 (d, IH), 8.37 (d, IH), 8.14 (d, IH), 8.00 (m, 2H), 7.80-7.66 (m, 4H), 7.60 (m, IH), 7.48-7.22 (m, 5H), 5.53 (d, J=5.3 Hz, IH), 3.94 (m, IH), 2.45 (m, IH), 1.35 (d, 3H), 0.89-0.74 (m, 4H). 0 APCI-MS m/z: 517 [MH+]
(lR,2S)-l-(l-(4-fluorophenyl)-lH-indazol-5-yloxy)-l-(quinolin-3-yl)propan-2-amine bis (2, 2, 2-trifluoroacetate) (42a)
Figure imgf000065_0002
Following the procedure described in Exampel 1. Starting from (li?,25)-2-amino-l- (quinolin-3-yl)propan-l-ol dihydrochloride (42b, 250 mg, 0.80 mmol), l-(4-fluorophenyl)- 5-iodo-lH-indazole (340 mg, 1.01 mmol), Cs2CO3 (1070 mg, 3.28 mmol) and CuI (36 mg, 0.19 mmol) in butyronitrile (4 mL), the reaction vessel was sealed and flushed with argon,
5 the resulting slurry was stirred at +125 0C for 5 hours, the temperature was then lowered to 1000C and the mixture was stirred over night 16 hours. Workup and purification by HPLC afforded the subtitle compound as a hygroscopic yellow powder. Yield 200 mg (39%) 1H-NMR(SOO MHz, DMSO-d6): δ 9.01 (d, IH), 8.41 (d, IH), 8.26 (brs, 3H), 8.17 (d, IH), 8.02 (t, 2H), 7.84-7.68 (m, 4H), 7.64 (m, IH), 7.44-7.34 (m, 3H), 7.28 (d, IH), 5.89 (d,o J=3.32 Hz, IH), 3.95 (m, IH), 1.26 (d, 3H) APCI-MS m/z: 413.1[MH+ -2TFA]
(lR,2S)-2-amino-l-(quinolin-3-yl)propan-l-ol dihydrochloride (42b)
Figure imgf000066_0001
s Following the procedure described for preparation of 29b. Starting from {S)-tert-buty\ 1- oxo-l-(quinolin-3-yl)propan-2-ylcarbamate (42c, 1.6 g, 5.33 mmol), Aluminium isopropoxide (0.68 g, 3.33 mmol) and 2-propanol (4.5 mL, 59.16 mmol) in toluene (7 mL) stirred at +50 0C in sealed reaction tube flushed with argon for 16 hours. Work up and deprotection of the intermediate BOC-protected amine afforded the subtitle compound as ao colourless solid. Yield 1.29 g (88%).
Η-NMR(400 Mhz, DMSO-dό): δ 9.23 (d, IH), 8.97 (s, IH), 8.42-8.24 (m, 5H), 8.06 (t, IH), 7.89 (t, IH), 6.68 (vbrs,lH), 5.28 (d, J=3.72 Hz, IH), 3.68 (m, IH), 1.10 (d, 3H). APCI-MS m/z: 203 [MH+ -2HC1] 5 (S)-tert-butyl l-oxo-l-(quinolin-3-yl)propan-2-ylcarbamate (42c)
Figure imgf000067_0001
(S)-tert-butyl l-(methoxy(methyl)amino)-l-oxopropan-2-ylcarbamate (2.5 g, 10.76 mmol) was suspended in THF (5mL) and stirred at -1O0C, isopropylmagnesium chloride 2.0M solution in THF (5.4 ml, 10.80 mmol) was added and a solution was formed. To this solution was added a solution of Lithium tri(3-quinolinyl)magnesiate in THF/Hexane, prepared from 3-bromoquinoline (1.471 ml, 10.81 mmol) according to the procedure described by Sylvain Dumouchel et-al. in Tetrahedron 59 (2003) 8629-8640. The mixture was stirred at -1O0C for 30 minutes and was the allowed to reach room temperature and stirred over night, 15 h. The reaction mixture, a clear red solution, was slowly poured into ice-cooled IM HCl (aq) (100 mL). EtOAc (150 mL) was added and the mixture was stirred for a few minutes, the water phase was extracted once with EtOAc, the combined EtOAc solutions was further washed with saturated NaHCO3 (aq) and brine. The crude material was purified by flash-chromatography on silica using a gradient of 0% to 40% EtOAc in Heptane. The obtained material was the further purified by HPLC to afford the subtitle compound as a yellow sticky oil. Yield 1.6 g (49%)
1H-NMR(400 Mhz, CDC13): δ 9.44 (d, IH), 8.81 (s, IH), 8.20 (d, IH), 7.98 (d, IH), 7.89 (t, IH), 7.67 (t, IH), 5.53 (brd, IH), 5.42 (m, IH), 1.48 (d, 3H), 1,47 (s, 9H). APCI-MS m/z: 301.1 [MH+]
Example 43
N-((lR,2S)-l-(2,3-dihydrobenzo[b][l,4]dioxin-6-yl)-l-(l-(4-fluorophenyl)-lH-indazol-5- yloxy)propan-2-yl)cyclopropanesulfonamide
Figure imgf000067_0002
(IR,2S)- 1 -(2,3-dihydrobenzo[b][ 1 ,4]dioxin-6-yl)- 1 -( 1 -(4-fluorophenyl)- 1 H-indazol-5- yloxy)propan-2-amine (43a, 102 mg, 0.24 mmol) and DIPEA (170 μL, 0.97 mmol) was dissolved in NMP (2 mL). cyclopropanesulfonyl chloride (40 μL, 0.39 mmol) was added and the reaction mixture was stirred at room temperature for 1 hour.The reaction mixture was diluted with water (10 mL) and extracted with EtOAc (2x 10 mL), the organic phase was washed with brine, dried (Na2SO4), filtered and evaporated to give an oily residue s (NMP residues). The crude material was further purified by HPLC. Yield 80 mg (62%) 1H-NMR (300 MHz, DMSO-d6): δ 8.18 (d, IH), 7.79-7.67 (m, 3H), 7.46-7.32 (m, 3H), 7.21 (dd, IH), 7.12 (d, IH), 6.89-6.81 (m, 3H), 5.21 (d, IH), 4.20 (s, 4H), 3.67 (m, IH), 2.39 (m ,1H), 1.22 (d, 3H), 0.90-0.81 (m, 4H). APCI-MS m/z: 524.1 [MH+] 0
(lR,2S)-l-(2,3-dihydrobenzo[b][l,4]dioxin-6-yl)-l-(l-(4-βuorophenyl)-lH-indazol-5- yloxy)propan-2-amine trtfluoroacetate (43a)
Figure imgf000068_0001
The subtitle compound was prepared following the procedure described in Exampel 1.s Starting from (lR,2S)-2-amino-l-(2,3-dihydrobenzo[b][l,4]dioxin-6-yl)propan-l-ol hydrochloride (43b, 1.46 g, 5.94 mmol), l-(4-fluorophenyl)-5-iodo-lH-indazole (2.4 g, 7.10 mmol), cesium carbonate (5.8 g, 17.80 mmol) and CuI (0.23 g, 1.21 mmol) in butyronitrile (18 mL). The reaction tube was capped and flushed with argon, the reaction mixture was stirred at +100 0C for 16 hours. Final purification was made by HPLC. Yield0 1.16 g (36%)
1H-NMR (300 MHz, DMSO-d6): δ 8.22 (d, IH), 8.13 (brs, 3H), 7.79-7.69 (m, 3H), 7.41 (m, 2H), 7.27 (dd, IH), 7.15 (d, IH), 6.94-6.82 (m, 3H), 5.51 (d, J=3.32 Hz, IH), 4.21 (s, 4H), 3.68 (m, IH), 1.17 (d, 3H) APCI-MS m/z: 420.1 [MH+-TFA]
(lR,2S)-2-amino-l-(2,3-dihydrobenzo[b] [1 ,4]dioxin-6-yl)propan-l-ol hydrochloride (43b)
Figure imgf000069_0001
Following the procedure described for preparation of 29b. Starting from (S)-tert-buty\ 1-
(2,3-dihydrobenzo[b][l,4]dioxin-6-yl)-l-oxopropan-2-ylcarbamate (43c, 3.76 g, 12.23 mmol), 2-propanol (12 mL, 157.75 mmol) and aluminium isopropoxide (0.5 g, 2.45 mmol) in toluene (22 mL) stirred at +50 °C under argon for 16 hours. Work up and purification by flash-chromatography. EtOAc : Hexane (1 : 2) as eluent afforded 3.19 g (84%) of the intermediate BOC-protected subtitle compound. APCI-MS m/z: 236, 210, 192, compound not stable in LC/MS system.
Deprotection of the BOC group afforded the subtiltle compound as a hygroscopic salt, Yield 2.1O g (70%)
1H-NMR (300 MHz, DMSO-d6): δ 8.01 (brs, 3H), 6.87-6.75 (m, 3H), 5.93 (brd, IH), 4.79
(brt, IH), 4.22 (s, 4H), 3.32 (m, IH), 0.95 (d, 3H).
APCI-MS m/z: 210 [MH+-HCl]
(S)-tert-butyl l-(2,3-dihydrobenzo[b][l,4]dioxin-6-yl)-l-oxopropan-2-ylcarbamate (43c)
Figure imgf000069_0002
Isopropylmagnesium chloride, 2M in THF (6.5 mL, 13.00 mmol) was added to a suspension of (S)-tert-butyl l-(methoxy(methyl)amino)-l-oxopropan-2-ylcarbamate (3 g, 12.92 mmol) in THF (30 mL) keeping the temperature below -1O0C. (2,3- dihydrobenzo[b][l,4]dioxin-6-yl)magnesium bromide, 0.7M in THF (20 mL, 14.00 mmol) was added. The reaction mixture was stirred at room temperature for 17 hours. IN HCl (300 mL) was cooled on icebath to +1O0C, the reaction mixture was poured into the acidic water solution and extracted with TBME = tert-butyl methyl ether. The ether phases were washed with water, brine and dried (Na2SO4). Filtration and evaporation of solvents afforded a crude product as a slightly yellow oil that was purified by flash chromatography using TBME : Heptane = 1 :2 as eluent. Yield 3.76g (95%) as a slightly yellow sticky oil/gum.
IH-NMR (300 MHz, DMSO-dό): δ 7.50 (dd, IH), 7.46 (d, IH), 7.23 (d, IH), 6.97 (d, IH), 4.97 (m, IH), 4.30 (m, 4H), 1.36 (s, 9H), 1.19 (d, 3H). APCI-MS m/z: 208 [MH+-BOC]
Example 44
Cyclopropanesulfonic acidN-{l-[6-methoxypyridin-3-yl]-l-[(l-pyridin-2-yl-lH-indazol-5- yl)oxy]propan-2-yl}amide
Figure imgf000070_0001
To a stirred solution of l-[6-methoxypyridin-3-yl]-l-[l-(pyridin-2-yl)indazol-5-yl]oxypropan- 2-amine (50 mg, 130 μmol) in dichloromethane (5 mL) was added triethylamine (44 μl), followed by cyclopropane sulfonic acid chloride (28 mg , 200 μmol) and DMAP (1.6 mg, 13 μmol). The stirring was continued for 20 min at room temp The reaction mixture was poured into sat. NH4Cl solution and extracted with dichloromethane then the solvent was removed i.vac, and the product purified by chromatography on silica gel. Yield 24 mg as a racemic mixture of two diastereomeres (37 %). ESI+MS: m/z 480 [MH+] 1H-NMR (CDCl3); δ = 8.74 (d, IH), 8.49 (d, IH), 8.24 (d, 0.5H), 8.20 (d, 0.5H), 8.01 (s, IH), 8.00 (d, IH), 7. 80 (ddd, IH), 7.66 (dd, 0.5H), 7.59 (dd, 0.5H), 7.21 (dd, 0.5H)3 7.18 (dd, 0.5H), 7.13 (m, IH), 7.0 (d, 0.5H), 6.98 (d, 0.5H), 6.75 (d, IH), 5.42 (d, 0.5H), 5.22 (d, 0.5H), 4.66 (d, 0.5H), 4.58 (d, 0.5H), 3.94 (m, IH), 3.92 (s, 3H), 2.43 (dddd, 0.5H), 2.18 (dddd, 0.5H), 1.45 (d, 1.5H), 1.33 (d, 1.5H), 1.20-0.78 (m, 4H).
1 -[6-Methoxypyridin-3-yl]-l-[l - (pyridin-2-yl) indazol-5-yl) 'oxypropan-2 -amine
Figure imgf000071_0001
The title compound was prepared essentially by the method described by Job & Buchwald: Org. Lett. 2002, 4 (21), 3703-3706.
5-Iodo-l-(2-pyridinyl)indazole ( 2.82 g, 8.8 mmole), 2-Amino-l-[6-methoxypyridin-3- 5 yl]propan-2-ol (1.6 g, 8.8 mmol, mixture of diastereomers), copper(I)iodide (167 mg, 0.88 mmol) and caesium carbonate (5.72 g, 17.6 mmole) was suspended in butyronitrile (6 mL) and toluene (12 mL). The reaction vessel was capped and the mixture was stirred at 130 °C for three days. The reaction mixture was diluted with dichloromethane and filtered through a path of cellites. Then the solvent was removed i.vaα, and the product purified by chromatography io on silica gel. Yield 1.2 g (36 %) as a racemic mixture of two diastereomeres. ESI+MS: m/z 376 [MH+]
1H-NMR (CDCl3); δ = 8.70 (d, 0.5H), 8.68 (d, 0.5H), 8.47 (d, IH), 8.20 (d, 0.5H), 8.17 (d, 0.5H), 7.99 (s, IH), 7.98 (d, IH), 7. 79 (ddd, IH), 7.62 (dd, 0.5H), 7.59 (dd, 0.5H), 7.20 (dd, IH), 7.12 (m, IH), 7.03 (d, 0.5H), 6.98 (d, 0.5H), 6.75 (d, IH), 5.08 (br., 0.5H), 4.91
I5 (br., 0.5H), 3.91 (s, 3H), 3.50 (br., 3H), 1.24 (br., 1.5H), 1.11 (br., 1.5H).
2-Amino-l-[6-methoxypyridin-3-yl]propan-2-ol
Figure imgf000071_0002
l-(6-methoxypyridin-3-yl)-2-nitro-propan-l-ol (2.2Og, 10.37 mmol) was dissolved ino methanol (410 mL) and hydrogenated using a H-Cube™ hydrogenation reactor (THALES nanotechnology) equipped with a cartridge of 10% Pd/C. The flow rate was set to 0.8 mL/min, temperarure 80°C and full the hydrogen production at full mode. After evaporation of the solution diastereomers can be separeted on preparative HPLC (XTerrra C18, 19x50 mm) using a gradient of 5-30% acetonitrile in water (+1% NH3) gave the pure5 subtitle compound (448 mg, 24%). 1H-NMR (400 MHz, OMSO-d6): δ = 8.05 (IH, d);7.63 (IH, dd);6.76 (IH, d);4.29 (IH, d);3.82 (3H, s);2.90 (IH, quintet);0.87 (3H, d). APCI-MS: m/z 183.0 [MH+].
l-(6-methoxypyridin-3-yl)-2-nitro-propan-l-ol
Figure imgf000072_0001
To a round bottom flask was added anhyrous magnesium sulphate (4.77g, 40mmol) and nitroethane (15ml) The flask was evacuated and filled with argon. The reaction mixture was stirred vigorously to get a homogeneous suspension before 6-methoxynicotinaldehyde (2.37g, 18mmol in 5mL nitroethane) was added. After stirring in 5 min 2,5,8,9-tetraaza-l- phosphabicyclo[3.3.3]undecane,2,8,9-tris(l-methylethyl) (1082mg,3.6mmol) was added. The reaction mixture was stirred overnight at room temperature before it was purified by flash chromatography (SiO2, heptane-ethylacetate). Yield 2.22 g, 58 %. APCI-MS: m/z 213.1 [MH+].
5-Iodo-l- (2-pyridinyl) indazole
Figure imgf000072_0002
Cesiumcarbonate (29.86 g, 91.6 mmol) is added to a suspension of 2-fluoro-5- iodobenzaldehyde (11.45 g, 45.8 mmol) and 2-pyridylhydrazine (5 g, 45.8 mmol) in 230 mL N-methylpyrrolidon. The reaction mixture is stirred two hours at room temperature. After checking that the hydrazone has been formed (1H-NMR) the reaction mixture is heated for three hours at 150 °C. The reaction mixture is allowed to cool down and the darkbrown suspension is poured into ice water. After vigorously stirring at room temperature for 15 minutes the mixture is extracted with ethyl acetate. The organic phase is washed with brine, dried over Na2SO4, the solvent is removed i.vac, and the product purified by chromatography on silica gel. Yield 4.8 g (33 %). ES+MS : 322 (MH+) 1H-NMR (CDCl3); δ = 7.18 (ddd, IH), 7.75 (dd, IH), 7.84 (ddd, IH), 8.05 (d, IH), 8.11 (s, IH), 8.14 (d, IH), 8.53 (m, IH), 8.66 (IH).
Example 45
Cyclopropanesulfonic acidN-{l-[6-methoxypyridin-3-yl]-l-[(l-pyridin-3-yl-lH-indazol-5- yl)oxy]propan-2-yl}amide
Figure imgf000073_0001
To a stirred solution of l-[6-methoxypyridin-3-yl]-l-[l-(pyridin-3-yl)indazol-5-yl]oxypropan- 2-amine (45 mg, 120 μmol) in dichloromethane (4.5 mL) was added triethylamine (40 μl), followed by cyclopropane sulfonic acid chloride (25 mg , 180 μmol) and DMAP (1.5 mg, 12 μmol). The stirring was continued for 20 hours at room temp. The reaction mixture was poured into sat. NH4Cl solution and extracted with dichloromethane, then the solvent was removed i.vac, and the product purified by chromatography on silica gel. Yield 4 mg as a racemic mixture of two diastereomeres (7 %). ES+MS: m/z 480 [MH+]
1H-NMR (CDCl3); δ = 9.03 (br., IH), 8.60 (br., IH), 8.24 (d, 0.5H), 8.20 (d, 0.5H), 8.07 (s, 0.5H), 8.06 (s, 0.5H), 8.03 (d, IH), 7. 66 (dd, 0.5H), 7.64 (d, IH), 7.59 (dd, 0.5H), 7.48 (ddd, IH), 7.19 (dd, 0.5H), 7.16 (dd, 0.5H), 7.05 (d, 0.5H), 7.02 (d, 0.5H), 6.76 (d, IH), 5.42 (d, 0.5H), 5.22 (d, 0.5H), 4.71 (d, 0.5H), 4.60 (d, 0.5H), 3.93 (s, 3H), 3.92 (m, IH), 2.44 (dddd, 0.5H), 2.19 (dddd, 0.5H), 1.43 (d, 1.5H), 1.33 (d, 1.5H), 1.24 - 0.78 (m, 4H)3 .
l-[6-Methoxypyridin-3-yl]-l-[l-(pyridin-3-yl)indazol-5-yl]oxypropan-2-amine
Figure imgf000073_0002
5-Iodo-l-(3-pyridinyl)indazole (750 mg, 2.3 mmole), 2-Amino-l-[6-rnethoxypyridin-3- yl]propan-2-ol (410 mg, 2.2 mmol), copper(I)iodide (42 mg, 0.22 mmol) and caesium carbonate (1.45 g, 4.5 mmole) were suspended in butyronitrile (2.4 mL) and toluene (4.8 mL). The reaction vessel was capped and the mixture was stirred at 130 °C for 20 hours. Then the solvent was removed i.vaα, and the product purified by chromatography on silica gel. Yield 254 mg (30%) as a racemic mixture of two diastereomeres. 5 ESI+MS: m/z 376 [MH+]
1H-NMR (CDCl3); δ = 9.01 (br., IH), 8.57 (d, IH), 8.19 (d, 0.5H), 8.17 (d, 0.5H), 8.04 (s, IH), 8.01 (m, IH), 7. 59 (m, 2H), 7.46 (ddd, IH), 7.18 (d, 0.5H), 7.14 (d, 0.5H), 7.05 (d, 0.5H), 7.02 (d, 0.5H), 6.75 (d, IH), 5.16 (d, 0.5H), 5.15 (d, 0.5H), 3.93 (s, 3H), 3.13 (dq, 0.5H), 3.11 (dq, 0.5H), 1.20 (d., 1.5H), 1.10 (d, 1.5H). 0
5-Iodo-l-pyridin-3-yllH-indazole
Figure imgf000074_0001
Cesiumcarbonate (26.84 g, 82.38 mmol) is added to a suspension of 2-fluoro-5- iodobenzaldehyde (6.87 g, 27.46 mmol) and 3-pyridylhydrazine dihydrochloride (5 g,s 27.46 mmol) in 136 mL N-methylpyrrolidon. The reaction mixture is stirred overnight at room temperature. After checking that the hydrazone has been formed (1H-NMR) the reaction mixture is heated for four hours at 160 °C. The reaction mixture is allowed to cool down and the darkbrown suspension is poured on 1000 mL ice water. After vigorously stirring at room temperature for 45 minutes, the precipitated product is sucked off via ao glass microfibre filter, washed with water and dried at the evaporator at 45 °C. 8.28 g (93.9%) of the title compound are obtained. MS (CI+): m/z 322 (M+)
1H-NMR (400 MHz, DMSO [d6]): δ = 7.62 (IH), 7.72 (2H), 8.20 (IH), 8.32 (IH), 8.49 (IH), 8.61 (IH), 9.01 (IH). 5
Example 46
Cyclopropanesulfonic acidN-{l-[2-methoxypyridin-4-yl]-l-[(l-pyridin-2-yl-lH-indazol-5- yl)oxy]propan-2-ylj amide
Figure imgf000075_0001
To a stirred solution of l-[2-methoxypyridin-4-yl]-l-[l-(pyridin-2-yl)indazol-5-yl]oxypropan- 2-amine (41 mg, 110 μmol) in dichloromethane (4 ml) was added triethylamine (36 μl), followed by cyclopropanesulfonic acid chloride (23 mg , 160 μmol) and DMAP (1.3 mg,
5 11 μmol). The stirring was continued for 20 h at room temp. The reaction mixture was poured into sat. NH4Cl solution and extracted with dichloromethane then the solvent was removed i.vac, and the product purified by chromatography on silica gel. Yield 4 mg (7.6 %) as a racemic mixture of one diastereomer. ES+MS: m/z 480 [MH+] io 1H-NMR (CDCl3); δ = 9.04 (d, IH), 8.61 (d, IH), 8.18 (d, IH), 8.06 (s, IH), 7.66 (d, IH), 7.49 (dd, IH), 7.19 (dd, IH), 6.97 (d, IH), 6.90 (dd, IH), 6.76 (s, IH), 5.42 (d,lH), 4.74 (d, IH), 3.95 (m, IH), 3.92 (s, 3H), 2.44 (dddd, IH), 1.29 (d, 3H), 1.21 (m, IH), 1.01 (m, 3H).
l-[2-Methoxypyridin-4-yl]-l-[l-(pyridin-2-yl)indazol-5-yl]oxypropan-2-amine
Figure imgf000075_0002
The title compound was prepared essentially by the method described by Job & Buchwald:
Org. Lett. 2002, 4 (21), 3703-3706.
5-Iodo-l-(3-pyridinyl)indazole ( 750 mg, 2.3 mmole), 2-Amino-l-[6-methoxypyridin-3- yl]propan-2-ol (410 mg, 2.2 mmol), copper(I)iodide (42 mg, 0.22 mmol) and caesium 2o carbonate (1.45 g, 4.5 mmole) were suspended in butyronitrile (2.4 mL) and toluene (4.8 mL).
The reaction vessel was capped and the mixture was stirred at 130 0C for 20 hours. Then the solvent was removed i.vac, and the product purified by chromatography on silica gel. Yield
243 mg (30 %) as a racemic mixture of one diastereomere.
1H-NMR (CDCl3); δ = 9.01 (d, IH), 8.58 (dd, IH), 8.12 (d, IH), 8.04 (s, IH), 8.02 (m, 5 IH), 7.63 (d, 2H), 7.46 (dd, IH), 7.18 (dd, IH), 6.96 (d, IH), 6.85 (dd, IH), 6.72 (s, IH),
4.14 (d, IH), 3.93 (s, 3H), 3.12 (dq, IH), 1.27 (d, 3H). 2-Amino-l-[2-methoxypyridin-4-yl]propan-2-ol
Figure imgf000076_0001
Ammonium formate (1.13 g, 18 mmol) and palladium on charcoal (10%, 175 mg) is added to a solution of l-(2-methoxypyridin-4-yl)τ2-nitro-propan-l-ol (760 mg, 3.6 mmol) inl7.5 ml THF and 17.5 ml methanol. The reaction mixture is stirred overnight at room temperature, filtered through a path of cellites and concentrated. Yield 620 mg of a racemic mixture of two diastereomers, 95 %.
1H-NMR (CDCl3); δ = 8.11 (d, IH), 6.85 (d, 0.5H), 6.83 (d, 0.5H), 6.74 (s, 0.5H), 6.73 (s, 0.5H), 4.53 (d, 0.5H), 4.22 (d, 0.5H), 3.93 (s, 3H), 3.24 (dq, 0.5H), 3.03 (d, 0.5H), 1.63 (br. 2H), 1.11 (d, 1.5H), 0.90 (d, 1.5H).
l-(2-Methoxypyridin-4-yl)-2-nitropropan-l-ol
Figure imgf000076_0002
To a round bottom flask was added anhyrous magnesium sulfate (0.97 g, 8 mmol) and nitroethane (1.34 ml, 18.7 mmol) The flask was evacuated and filled with argon. The reaction mixture was stirred vigorously to get a homogeneous suspension before 2- methoxypyridine-4- carboxaldehyde (500 mg, 3.65 mmol) was added. After stirring in 5 min 2,5,8,9-tetraaza-l-phosphabicyclo[3.3.3]undecane,2,8,9-tris(l-methylethyl) (109 mg, 0.36 mmol) was added. The reaction mixture was stirred overnight at room temperature before it was purified by flash chromatography (SiO2, diethyl ether). Yield 770 mg of a racemic mixture of two diastereomers, 99 %.
1H-NMR (CDCl3); δ = 8.17 (d, 0.5H), 8.15 (d, 0.5H), 6.87 (d, 0.5H), 6.85 (d, 0.5H), 6.80 (s, 0.5H), 6.75 (s, 0.5H), 5.42 (d, 0.5H), 4.98 (d, 0.5H), 4.73 (dq, 0.5H), 4.67 (d, 0.5H), 3.94 (s, 3H), 1.47 (d, 1.5H), 1.40 (d, 1.5H).
Example 47 Cyclopropanesulfonic acidN-{l-[2-methoxypyridin-4-yl]-l-[(l-pyridin-2-yl-lH-indazol-5- yl)oxy] ' butan-2-yl} amide
Figure imgf000077_0001
To a stirred solution of l-[2-methoxypyridin-4-yl]-l-[(l-pyridin-2-yl-lH-indazol-5- s yl)oxy]butan-2-amine (122 mg, 310 μmol) in dichloromethane (11 ml) was added triethylamine (100 μl), followed by cyclopropanesulphonic acid chloride (66 mg , 470 μmol) and DMAP (3,8 mg, 31 μmol). The stirring was continued for 48 h at room temp. The reaction mixture was poured into sat. NH4Cl solution and extracted with dichloromethane then the solvent was removed i.vac, and the product purified by chromatography on silica gel. Yieldo 10 mg (6,5 %) as a racemic mixture of two diastereomeres. ESI+MS: m/z 494 [MH+]
1H-NMR (CDCl3); δ = 8.76 (d, IH), 8.49 (d, IH), 8.21 (d, 0.5H), 8. 16 (d, 0.5H), 8.02 (s, IH), 8.00 (d, IH), 7.81 (ddd, IH), 7.19 (dd, IH), 7.13 (m, IH), 6.94 (m, 2H), 6.83 (br, 0.5H), 6.77 (br. 0.5H), 5.47 (d, 0.5H), 5.29 (d, 0.5H), 4.62 (d, 0.5H), 4.56 (d, 0.5H), 3.94s (m, IH), 3.91 (s, 3H), 2.47 (dddd, 0.5H), 2.28 (dddd, 0.5H), 1.94 (m, IH), 1.64 (m, IH), 1.22 (t, 1.5H), 1.09 (t, 1.5H), 0,98 (m, 2.5H), 0.78 (m, 0.5H).
l-[2-Methoxypyridin-4-yl]-l-[l-(pyridin-2-yl)indazol-5-yl]oxybutan-2-amine
Figure imgf000077_0002
0 The title compound was prepared essentially by the method described by Job & Buchwald: Org. Lett. 2002, 4 (21), 3703-3706.
5-Iodo-l-(2-pyridinyl)indazole ( 830 mg, 2.6 mmol), 2-amino-l-[2-methoxypyridin-4- yl]butan-2-ol (510 mg, 2.6 mmol), copper(I)iodide (49 mg, 0.26 mmol) and caesium carbonate (1.68 g, 5.2 mmol) were suspended in butyronitrile (1.7 mL) and toluene (3.5 mL). The reaction vessel was capped and the mixture was stirred at 130 0C for 60 hours. Then the solvent was removed i.vac, and the product purified by chromatography on silica gel. Yield 680 mg (67 %) as a racemic mixture of two diastereomeres.
1H-NMR (CDCl3); δ = 8.71 (d, IH), 8.48 (d, IH), 8.15 (d, 0.5H), 8.11 (d, 0.5H), 8.00 (s, IH), 7.99 (d, IH), 7.80 (dd, IH), 7.22 (dd, IH), 7.13 (dd, IH), 6.94 (m, 2H), 6.77 (s, 0.5H), 6.72 (s, 0.5H), 5.22 (d, 0.5H), 5.05 (d, 0.5H), 3.93 (s, 3H), 3.12 (m, 0.5H), 2.98 (m, 0.5H), 2.36 (br., 2H) 1.60 (m, 2H), 1.02 (t, 1.5H), 0.98 (t, 1.5H).
2- Amino- 1 -[2-methoxypyridin-3-yl]butan-2-ol
Figure imgf000078_0001
o Ammonium formate (2.5 g, 40 mmol) and palladium on charcoal (10%, 390 mg) is added to a solution of l-(2-methoxypyridin-4-yl)-2-nitrobutan-l-ol (1.7 g, 7.5 mmol) in 38 ml THF and 38 ml methanol. The reaction mixture is stirred overnight at room temperature, filtered through a path of cellites and concentrated. Yield 620 mg of a racemic mixture of two diastereomers, 42%. s 1H-NMR (CDCl3); δ = 8.11 (d, 0.5H), 8.10 (d, 0.5H), 6.84 (d, 0.5H), 6.83 (d, 0.5H), 6.74 (s, 0.5H), 6.72 (s, 0.5H), 4.61 (d, 0.5H), 4.31 (d, 0.5H), 3.92 (s, 3H), 2.95 (ddd, 0.5H), 2.77 (ddd, 0.5H), 2.33 (br. 2H), 1.55 (m, 0.5H), 1.38 (m, 0.5H), 1.30 (m, 0.5H), 1.12 (m, 0.5H), 0.96 (t, 1.5H), 0.90 (t, 1.5H). o l-(2-Methoxypyridin-4-yl)-2-nitrobutan-l-ol
Figure imgf000078_0002
To a round bottom flask was added anhyrous magnesium sulphate (3.09 g, 25.7 mmol) and nitro propane (5.35 ml, 60 mmol) The flask was evacuated and filled with argon. The reaction mixture was stirred vigorously to get a homogeneous suspension before 2-5 methoxypyridine-4- carboxaldehyde (500 mg, 3.65 mmol) in 5 ml was added. After stirring in 5 min 2,8,9-tris(l-methylethyl)-2,5,8,9-tetraaza-l- phosphabicyclo[3.3.3]undecane (350 mg, 1.2 mmol) was added in 5 ml nitro propan. The reaction mixture was stirred overnight at room temperature before it was purified by flash chromatography (SiO2, diethyl ether). Yield 1.7 g of a racemic mixture of two diastereomers, 64 %.
1H-NMR (CDCl3); δ = 8.17 (d, 0.5H), 8.15 (d, 0.5H), 6.87 (d, IH), 6.79 (s, 0.5H), 6.74 (s, 5 0.5H), 5.19 (d, 0.5H), 4.98 (d, 0.5H), 4.58 (ddd, 0.5H), 4.55 (ddd, 0.5H), 3.94 (s, 3H), 2.16 (m, 0.5H), 1.90 (m, 0.5H), 1.78 (m, 0.5H), 1.55 (m, 0.5H), 0.93 (t, 1.5H), 0.92 (t, 1.5H).
Example 48 l-Methylimidazole-4-sulfonic acid N-[(1R, 2S)-l-[l-(4-βuorophenyl)indazol-5-yl]oxy-l-o phenyl-propan-2-yl] amide
Figure imgf000079_0001
To (ii?,2ιS)-l-[l-(4-fluorophenyl)indazol-5-yl]oxy-l-phenylpropan-2-amine (Ia, 60 mg, 0.14 mmol) 130 μmol) in dichloromethane (6 mL) was added triethylamine (55 μl), followed by 1- methylimidazole-4-sulfonyl chloride (36 mg , 200 μmol) and DMAP (2 mg, 17 μmol). Thes stirring was continued for 18 hours at room temperature and the reaction mixture was poured into water and extracted with dichloromethane. The combined organic phases were washed with brine, died over sodium sulphate, the solvent was removed i.vac, and the product purified by chromatography on silica gel. Yield 39 mg (46%). MS (CI+): m/z 506 (MH+). o 1H-NMR (300 MHz, CDCl3) δ = 7.93 (s, IH), 7.62 (dd, 2H), 7.53 (d, IH), 7.40 (d, 2H), 7.33 (m, 4H), 7.28 (t, IH), 7.20 (t, 2H), 7.14 (dd, IH), 6.85 (d, IH), 5.46 (d, IH), 5.24 (d, IH), 3.93 (dq, IH), 3.63 (s, 3H), 1.12 (d, 3H).
Example 49 5 3,5-Dimethylisooxazole-4-sulfonic acidN-[(lR,2S)-l-[l-(4-fluorophenyl)indazol-5-yl]oxy-l- phenyl-propan-2-yl] amide
Figure imgf000080_0001
To (7i?,2>S)-l-[l-(4-fluorophenyl)indazol-5-yl]oxy-l-phenylpropan-2-amine (Ia, 60 mg, 0.14 mmol) 130 μmol) in dichloromethane (6 mL) was added triethylamine (55 μl), followed by 3,5-dimethylisooxazole-4-sulfonyl chloride (39 mg , 200 μmol) and DMAP (2 mg, 17 μmol). The stirring was continued for 18 hours at room temperature and the reaction mixture was poured into water and extracted with dichloromethane. The combined organic phases were washed with brine, died over sodium sulphate, the solvent was removed i.vac, and the product purified by chromatography on silica gel. Yield 31 mg (36%). MS (CI+): m/z 521 (MH+). 1H-NMR (300 MHz, CDCl3) δ = 7.96 (s, IH), 7.62 (dd, 2H), 7.54 (d, IH), 7.35 (m, 3H), 7.28 (m, IH), 7.21 (t, 2H), 7.04 (dd, IH), 6.87 (d, IH), 5.26 (d, IH), 4.97 (d, IH), 3.77 (dq, IH), 2.64 (s, 3H), 2.38 (s, 3H), 1.22 (d, 3H).
Assay Human Glucocorticoid Receptor (GR) Assay
The assay is based on a commercial kit from Panvera/Invitrogen (Part number P2893). The assay technology is fluorescence polarization. The kit utilises recombinant human GR (Panvera, Part number P2812), a Fluoromone™ labelled tracer (GS Red, Panvera, Part number P2894) and a Stabilizing Peptide 1OX (Panvera, Part number P2815). The GR and Stabilizing Peptide reagents are stored at -70°C while the GS Red is stored at - 2O0C. Also included in the kit are IM DTT (Panvera, Part number P2325, stored at -200C) and GR Screening buffer 1OX (Panvera, Part number P2814, stored at -7O0C initially but once thawed stored at room temperature). Avoid repeated freeze/thaws for all reagents. The GR Screening buffer 1OX comprises 10OmM potassium phosphate, 20OmM sodium molybdate, ImM EDTA and 20% DMSO.
Test compounds (lμL) and controls (lμL) in 100% DMSO were added to black polystyrene 384-well plates (Greiner low volume black flat-bottom, part number 784076). 0% control was 100%DMSO and 100% control was lOμM Dexamethasone. Background solution (8μL; assay buffer 1OX, Stabilizing Peptide, DTT and ice cold MQ water) was added to the background wells. GS Red solution (7μL; assay buffer 1OX, Stabilizing Peptide, DTT, GS Red and ice cold water) was added to all wells except background wells. GR solution (7μL; assay buffer 10X, Stabilizing Peptide, DTT, GR and ice cold water) was added to all wells. The plate was sealed and incubated in a dark at room temperature for 2hours. The plate was read in an Analyst plate reader (LJL Biosystems/Molecular Devices Corporation) or other similar plate reader capable of recording fluorescence polarization (excitation wavelength 530nm, emission wavelength 59OnM and a dichroic mirror at 561nm). The IC50 values were calculated using XLfϊt model 205.
GRhuFL_FP_v2
Mean IC50
Example (nM)
1 6
2 6.9
3 90
4 4.9
5 2.3
6 5.9
7 4.6
8 4.6
9 3.2
10 6.5
11 6.9
12 2.8
13 5
14 4.3 5.4
6.4
5.7
4.3
5.1
8.7
9.9
2.7
9.5
7.6
7
77
6.3
6.3
21
330
6.4
7.6
170
2.5
6.2
4.3
17 4.8
280
13
250
3.6
2.3

Claims

1. A compound of formula (I):
Figure imgf000084_0001
wherein:
A is C1-10alkyl, C5-10aryl, C5-i0arylCi-6alkyl, C5-10heteroaryl, Cs-ioheteroarylCi-δalkyl, C5- ioarylCi-6alkoxy, d-iohaloalkyl, C3-7cycloalkyl, C3-7cycloalkylC1-4alkyl, C1- 6alkyl0C(0)Ci-6alkyl, C1-6alkylC(O)OC1-6alkyl , C5-10aryloxyd-ioalkyl or NR5R6C0-6alkyl whereby the aryl is optionally substituted with one or more substituents selected from B; R1 and Rla are independently selected from hydrogen, Ci-4alkyl, C1-4haloalkyl, Ci. 4hydroxyalkyl and Ci.4alkyl0Ci-4alkyl; R2 is hydrogen or Ci-4alkyl;
R3 is C3-7 cycloalkyl (optionally subtiruted by halogen or Ci-6 alkyl), C5.ioarylCo-3alkyl, C5. 10arylOCo-3alkyl,
Figure imgf000084_0002
Ci.6alkyl, Ci.6alkenyl or
Figure imgf000084_0003
which are optionally substituted by one or more B;
B is C0.3hydroxyalkyl, Ci-4alkyl, Ci-4alkoxy, Co^alkylthioCo^alkyl, d-βcycloalkylCo- 4thioalkyl, Co-3alkylS(0)nCo-4alkyl, Ci.6haloalkyl, C1-4haloalkoxy, halogen, nitro, cyano, CMalkylOd-ealkyl,
Figure imgf000084_0004
C0-6alkylC(O)C0.6alkyl, C0- 4alkylC(O)OC0-4alkyl, C0-4alkylOC(O)C0-4alkyl, NR5R6C0-4alkyl, NR5R6C(O)C0-4alkyl, NR5R6OC(O)C0-4alkyl, NR5R6C(O)OC0^alkyl, R6C(O)R5NC0-4alkyl, C0-4alkylOC(O)C0- 4alkylNH, C0-4alkylC(O)OC0-4alkylNH, C0-4alkylC(0)Co-4alkylNH Or NR5R6S(O)nC0- 4alkyl;
R4 is hydrogen, hydroxy, halogen, Ci-4 alkyl or Ci-4 haloalkyl; W is hydrogen, C3-7cycloalkyl, C^alkyl, phenyl, thienyl, isoxazolyl, pyrazolyl, pyridinyl or pyrimidinyl all optionally substituted with one or more substituents selected from halogen, C0-3hydroxyalkyl, Ci-4alkyl, Ci.4alkoxy, C0.4alkylthioCo-4alkyl, C3.6cycloalkylC0- 4thioalkyl, C0-4alkylS(0)nCo-4alkyl,
Figure imgf000084_0005
halo, nitro, cyano, Ci- 4alkyl0Ci-6alkyl, CLealkylOCLealkylOCi-ealkyl, C0-6aDcylC(0)Co.6alkyl, C0- 4alkylC(O)OC0-4alkyl, Co-4alkylOC(0)Co-4alkyl, NR5R6C0-4alkyl, NR5R6C(O)C0-4alkyl, NR5R6C(O)OC0-4alkyl, NR5R6OC(O)C0-4alkyl, R6C(O)R5NC0-4alkyl, C0-4alkylOC(0)Co. 4alkylNH, C0-4alkylC(O)OC0.4alkylNH, C0.4alkylC(O)C0-4alkylNH and NR5R6S(O)nC0- 4alkyl; X is CH2, O, S, S(O), S(O)2 or NH;
Y is hydrogen, halogen, C1-6 alkyl,
Figure imgf000085_0001
nitro, cyano, hydroxy, R5C(O), R5OC(O), R5C(O)O, S(O)nC1-4alkyl, R5R6NS(O)n, benzyloxy, imidazolyl, C1-4alkylNHC(O), NR5R6C(O),
Figure imgf000085_0002
Or NR5R6; R5 and R6 are independently selected from hydrogen, Ci-4 alkyl and C3-7 cycloalkyl, or R5 and R6 form together a group -(0)C5-ioarylC(0)-; and n is 1 or 2, or a pharmaceutically acceptable salt thereof.
2. A compound according to claim 1, wherein A is Ci-10alkyl, C5-1oaryl, C5-10arylC1-6alkyl, C5-i0heteroaryl, C5-ioheteroarylCi.6alkyl, C5-1oarylCi.6alkoxy, Ci.iohaloalkyl, C3-
7cycloalkyl, C3-7cycloalkylC1.4alkyl, Ci-6alkylOC(O)C]-6alkyl, Ci-6alkylC(O)OCi-6alkyl , Cs-ioaryloxyCi-ioalkyl or NR R6C0-6alkyl whereby the aryl is optionally substituted with one or more substituents selected from B.
3. A compound according to claim 1, wherein A is C3-6CyClOaIlCyI.
4. A compound according to any one of claims 1 to 3 wherein R3 is C5-ioarylCo-3alkyl, C5- ioarylOCo-3alkyl or Cs.ioheteroarylCo-salkyl which are optionally substituted by one or more B.
5. A compound according to any one of claims 1 to 3 wherein R3 is phenyl or R3 together with B form a dihydrobenzodioxinyl group.
6. A compound according to any one of claims 1 to 5 wherein W is C3-7cycloalkyl, Ci- 4alkyl, phenyl or pyridinyl all optionally substituted with one or more substituents selected from halogen.
7. A compound according to any one of claims 1 to 5 wherein W is phenyl substituted with fluoro.
8. A compound according to any one of claims 1 to 7 wherein R1 is C^alkyl, Rla is hhyyddrrooggeenn,, RR2 iiss hhyydrogen, X is O and R is C5-iOaryl,whereby aryl is optionally substituted by one or more B.
9. A compound selected from
N-[(1R,2S)- 1 -[ 1 -(4-fluorophenyl)indazol-5-yl]oxy- 1 -phenyl-propan-2- io yl]cyclopropanesulfonamide,
N-[( 1R,2S)- 1 -[ 1 -(4-fluorophenyl)indazol-5-yl]oxy- 1 -phenyl-propan-2-yl]propane- 1 - sulfonamide,
N-[(1R,2S)- 1 -[ 1 -(4-fluorophenyl)indazol-4-yl]oxy- 1 -phenyl-propan-2- yl]cyclopropanesulfonamide, s N-[(lR,2S)-l-[l-(6-fluoropyridin-3-yl)indazol-5-yl]oxy-l-phenyl-propan-2- yljcyclopropanesulfonamide,
N-[(lR,2S)-l-[l-(4-fluorophenyl)indazol-5-yl]oxy-l-phenyl-propan-2- yl]methanesulfonamide,
N-[(lR,2S)-l-[l-(4-fluorophenyl)indazol-5-yl]oxy-l-phenyl-propan-2-yl]-l-phenyl-0 methanesulfonamide,
1,1,1 -trifluoro-N-[( 1 R,2S)- 1 -[ 1 -(4-fluorophenyl)indazol-5-yl]oxy- 1 -phenyl-propan-2- yljmethanesulfonamide,
5-[(lR,2S)-2-(dimethylsulfamoylamino)-l-phenyl-propoxy]-l-(4-fluorophenyl)indazole,
N-[(lR,2S)-l-[l-(4-fluorophenyl)indazol-5-yl]oxy-l-phenyl-propan-2-yl]propane-2-5 sulfonamide,
2-( 1 ,3 -dioxoisoindol-2-yl)-N-[( 1 R,2S)- 1 -[ 1 -(4-fluorophenyl)indazol-5-yl]oxy- 1 -phenyl- propan-2-yl]ethanesulfonamide,
N-[( 1 R,2S)-1 -[ 1 -(4-fiuorophenyl)indazol-5-yl]oxy- 1 -phenyl-propan-2-yl]-3-(4- methoxyphenoxy)propane- 1 -sulfonamide, o N-[( 1 R,2S)- 1 -[ 1 -(4-fluorophenyl)indazol-5-yl]oxy- 1 -phenyl-propan-2- yl]ethanesulfonamide, N-[(1R,2S)- 1 -[ 1 -(4-fluorophenyl)indazol-5-yl]oxy-l -phenyl-propan-2-yl]pentane-2- sulfonamide,
N-[(l R,2S)- 1 -[ 1 -(4-fluorophenyl)indazol-5-yl]oxy- 1 -phenyl-propan-2-yl]butane-2- sulfonamide, N-[(lR,2S)-l-[l-(4-fluorophenyl)indazol-5-yI]oxy-l-phenyl-propan-2-yl]butane-l- sulfonamide,
N-[(lR,2S)-l-[l-(4-fluorophenyl)indazol-5-yl]oxy-l-phenyl-propan-2-yl]-2-methyl- propane- 1 -sulfonamide,
N-[(l R,2S)- 1 -[ 1 -(4-fluorophenyl)indazol-5-yl]oxy- 1 -phenyl-propan-2-yl]pentane-l - sulfonamide,
3 ,3 ,3-trifiuoro-N-[( 1 R,2 S)- 1 -[ 1 -(4-fluorophenyl)indazol-5-yl]oxy- 1 -phenyl-propan-2- yljpropane- 1 -sulfonamide, methyl 3-[[(l R,2S)-1 -[ 1 -(4-fluorophenyl)indazol-5-yl]oxy- 1 -phenyl-propan-2- yl]sulfamoyl]propanoate, l-cyclopentyl-N-[(lR,2S)-l-[l-(4-fluorophenyl)indazol-5-yl]oxy-l-phenyl-propan-2- yl]methanesulfonamide,
N-[(1R,2S)- 1 -[ 1 -(4-fluorophenyl)indazol-5-yl]oxy- 1 -phenyl-propan-2- yl] cyclopentanesulfonamide,
2,2,2-trifluoro-N-[(l R,2S)- 1 -[ 1 -(4-fluorophenyl)indazol-5-yl]oxy- 1 -phenyl-propan-2- yljethanesulfonamide, l-cyclohexyl-N-[(lR,2S)-l-[l-(4-fluorophenyl)indazol-5-yl]oxy-l-phenyl-propan-2- yl]methanesulfonamide,
N-[(lR,2S)-l-[l-(4-fluorophenyl)indazol-5-yl]oxy-l-phenyl-propan-2-yl]hexane-l- sulfonamide, N-[(lR,2S)-l-[l-(4-fluorophenyl)indazol-5-yl]oxy-l-phenyl-propan-2-yl]pyridine-3- sulfonamide,
N-[I-[I -(4- fluorophenyl)indazol-5-yl]oxy-2 -methyl- 1 -phenyl-propan-2- yl] cyclopropanesulfonamide,
N-[(lR,2S)-l-[l-(4-fluorophenyl)indazol-5-yl]oxy-l-(4-methylsulfanylphenyl)propan-2- yl]cyclopropanesulfonamide,
N-[(lS,2S)-l-[l-(4-fluorophenyl)indazol-5-yl]oxy-l-(4-methylsulfanylphenyl)propan-2- yljcyclopropanesulfonamide, N-[( 1 R,2S)- 1 -[ 1 -(4-fluorophenyl)indazol-5-yl]oxy- 1 -(4-methylsulfanylphenyl)propan-2- yljcyclopropanesulfonamide,
N-[( 1 R,2S)- 1 -phenyl- 1 -(I -propan-2-ylindazol-5-yl)oxy-propan-2-yl]methanesulfonamide,
N-[( 1 R,2S)- 1 -[ 1 -(4-fluorophenyl)indazol-5-yl]oxy- 1 -(4-methylsulfmylphenyl)propan-2- yl]cyclopropanesulfonamide,
N-[( 1 R,2S)- 1 -(I -cyclopentylindazol-S-ytyoxy- 1 -phenyl-propan-2- yl] cyclopropanesulfonamide,
N-[(lR,2S)-l-phenyl-l-(l-propan-2-ylindazol-5-yl)oxy-propan-2- yl] cyclopropanesulfonamide, N-[( 1 R,2S)- 1 -[ 1 -(4-fluorophenyl)indazol-5-yl]oxy-l -(4-methylsulfonylphenyl)propan-2- yl]cyclopropanesulfonamide,
N-[(lR,2S)-l-[6-chloro-l-(4-fluorophenyl)indazol-5-yl]oxy-l-(4-fluorophenyl)propan-2- yljcyclopropanesulfonamide,
N-[(lR,2R)-l-[6-chloro-l-(4-fluorophenyl)indazol-5-yl]oxy-l-(4-fluorophenyl)propan-2- yljcyclopropanesulfonamide,
N-[2-[l-(4-fluorophenyl)indazol-5-yl]sulfanyl-2-phenyl-ethyl]cyclopropanesulfonamide,
N-[(lR,2S)-l-[l-(4-fluorophenyl)indazol-5-yl]sulfanyl-l-phenyl-propan-2- yl]methanesulfonamide,
N-[( 1 R,2S)- 1 -[ 1 -(4-fluorophenyl)indazol-5-yl] sulfonyl- 1 -phenyl-propan-2- yl]methanesulfonamide,
N-[(2R)-2-[l-(4-fluorophenyl)indazol-5-yl]oxy-2-phenyl-ethyl]cyclopropanesulfonamide,
N-[(2S)-2-[l-(4-fluorophenyl)indazol-5-yl]oxy-2-phenyl-ethyl]cyclopropanesulfonamide,
N-[(lR,2S)-l-[l-(4-fluorophenyl)indazol-5-yl]oxy-l-quinolin-3-yl-propan-2- yljcyclopropanesulfonamide, N-[(lR.2S)-l-(2,5-dioxabicyclo[4.4.0]deca-7,9,l l-trien-8-yl)-l-[l-(4- fluorophenyl)indazol-5-yl]oxy-propan-2-yl]cyclopropanesulfonamide,
Cyclopropanesulfonic acid N- { 1 -[6-methoxypyridin-3-yl]-l -[( 1 -pyridin-2-yl- lH-indazol-
5-yl)oxy]propan-2-yl} amide,
Cyclopropanesulfonic acid N- { 1 -[6-methoxypyridin-3-yl]- 1 -[( 1 -pyridin-3-yl- lH-indazol- 5-yl)oxy]propan-2-yl} amide,
Cyclopropanesulfonic acid N-{l-[2-methoxypyridin-4-yl]-l-[(l-pyridin-2-yl-lH-indazol-
5-yl)oxy]propan-2-yl} amide, Cyclopropanesulfonic acid N- { 1 - [2-methoxypyridin-4-yl]- 1 - [( 1 -pyridin-2-yl- lH-indazol- 5-yl)oxy]butan-2-yl} amide, l-Methyl-lΗ-imidazole-4-sulfonic acid N-[(lR,2S)-l-[l-(4-fluorophenyl)indazol-5- yl]oxy- 1 -phenyl-propan-2-yl] amide, and 3,5-Dimethylisooxazole-4-sulfonic acid N-[(lR,2S)-l-[l-(4-fiuorophenyl)indazol-5- yl]oxy-l-phenyl-propan-2-yl]amide, or a pharmaceutically acceptable salt thereof.
10. A pharmaceutical composition comprising a compound of formula (I) or a pharmaceutically acceptable salt thereof as claimed in any one of claims 1 to 9, and a pharmaceutically acceptable adjuvant, diluent or carrier.
11. A compound of formula (I), or a pharmaceutically acceptable salt thereof, as claimed in in any one of claims 1 to 9 for use in therapy.
12. The use of a compound of formula (I), or a pharmaceutically acceptable salt thereof, as claimed i in any one of claims 1 to 9, in the manufacture of a medicament for use in the treatment of a glucocorticoid receptor mediated disease state.
13. The use of a compound of formula (I), or a pharmaceutically acceptable salt thereof, as claimed i in any one of claims 1 to 9, in the manufacture of a medicament for use in the treatment of an inflamatory condition.
14. The use of a compound of formula (I), or a pharmaceutically acceptable salt thereof, as claimed i in any one of claims 1 to 9, in the manufacture of a medicament for use in the treatment of an asthmatic condition.
15. The use of a compound of formula (I), or a pharmaceutically acceptable salt thereof, as claimed i in any one of claims 1 to 9, in the manufacture of a medicament for use in the treatment of COPD.
16. A method of treating a glucocorticoid receptor mediated disease state, an inflamatory condition, an asthmatic condition and/or COPD, in a mammal, which comprises administering to a mammal in need of such treatment an effective amount of a compound of formula (I), or a pharmaceutically acceptable salt thereof as claimed in any one of
5 claims 1 to 9.
17. A combination of a compound of formula (I), or a pharmaceutically acceptable salt thereof, as claimed in claim 1, and one or more agents selected from the list comprising:
I0 • a PDE4 inhibitor;
• a selective β.sub2. adrenoceptor agonist;
• a muscarinic receptor antagonist;
• a steroid;
• a modulator of chemokine receptor function; or, is • an inhibitor of p38 kinase function.
18. A process for the preparation of a compound of formula (I) by a) coupling a compound of formula (II):
Figure imgf000090_0001
0 with a compound of formula (III):
Figure imgf000090_0002
wherein L1 is a leaving group, in a suitable solvent, in the presence of a suitable base,
19. A process for the preparation of a compound of formula (II) by 5 a) when X is O, S or NH, coupling a compound of formula (IV)
Figure imgf000091_0001
wherein R4, W and Y are defined as in claim 1 and L2 is a leaving group with a compound of formula (V)
Figure imgf000091_0002
wherein R1, Rla and R2 are defined as in claim 1 and G corresponds to R3 or a protected precurser to R3, in a suitable solvent, in the presence of a suitable base ,
b) reaction of a compound of formula (VII)
Figure imgf000091_0003
with a compound of formula (VIII)
Figure imgf000091_0004
wherein R1, R2, R4, X, W and Y are defined as in claim 1, G corresponds to R3 or a protected precurser to R3 and L3 is a leaving group in a suitable solvent, in the presence of a suitable base, followed by a subsequent reductive amination step,
c) reaction of a compound of formula (VIII) and a compound of formula (IX)
Figure imgf000091_0005
wherein R1, Rla, R2 and R3 are defined as in claim 1 and PG is a suitable protecting group such as BOC, Ms, Ns, Ts or related carbonyl-or sulfonyl residues in a suitable solvent in the presence of a suitable base, followed by a deprotection step.
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