WO2008043046A2 - Clapets de non-retour microfluidiques - Google Patents

Clapets de non-retour microfluidiques Download PDF

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Publication number
WO2008043046A2
WO2008043046A2 PCT/US2007/080489 US2007080489W WO2008043046A2 WO 2008043046 A2 WO2008043046 A2 WO 2008043046A2 US 2007080489 W US2007080489 W US 2007080489W WO 2008043046 A2 WO2008043046 A2 WO 2008043046A2
Authority
WO
WIPO (PCT)
Prior art keywords
channel
microfluidic device
less
chamber
outlet
Prior art date
Application number
PCT/US2007/080489
Other languages
English (en)
Other versions
WO2008043046A3 (fr
Inventor
Jing Wang
Hany R. Nassef
Original Assignee
Fluidigm Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fluidigm Corporation filed Critical Fluidigm Corporation
Priority to EP07843872.8A priority Critical patent/EP2074341A4/fr
Publication of WO2008043046A2 publication Critical patent/WO2008043046A2/fr
Publication of WO2008043046A3 publication Critical patent/WO2008043046A3/fr

Links

Classifications

    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
    • F16K—VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING
    • F16K99/00—Subject matter not provided for in other groups of this subclass
    • F16K99/0001—Microvalves
    • F16K99/0003—Constructional types of microvalves; Details of the cutting-off member
    • F16K99/0026—Valves using channel deformation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
    • F16K—VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING
    • F16K99/00—Subject matter not provided for in other groups of this subclass
    • F16K99/0001—Microvalves
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
    • F16K—VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING
    • F16K99/00—Subject matter not provided for in other groups of this subclass
    • F16K99/0001—Microvalves
    • F16K99/0034—Operating means specially adapted for microvalves
    • F16K99/0055—Operating means specially adapted for microvalves actuated by fluids
    • F16K99/0057—Operating means specially adapted for microvalves actuated by fluids the fluid being the circulating fluid itself, e.g. check valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/12—Specific details about manufacturing devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0887—Laminated structure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0605—Valves, specific forms thereof check valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0633—Valves, specific forms thereof with moving parts
    • B01L2400/0638—Valves, specific forms thereof with moving parts membrane valves, flap valves
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
    • F16K—VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING
    • F16K99/00—Subject matter not provided for in other groups of this subclass
    • F16K2099/0073—Fabrication methods specifically adapted for microvalves
    • F16K2099/0078—Fabrication methods specifically adapted for microvalves using moulding or stamping
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
    • F16K—VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING
    • F16K99/00—Subject matter not provided for in other groups of this subclass
    • F16K2099/0073—Fabrication methods specifically adapted for microvalves
    • F16K2099/008—Multi-layer fabrications

Definitions

  • the ratio of C to A is equal to or less than about 1.5, equal to or less than about 1.75, equal to or less than about 2, equal to or less that about 2.5, equal to or less than about 3, or greater than 3.
  • the ratio of D to B can be equal to about 1.6 or less, equal to or less than about 1.8, equal to or less than about 2, equal to or less than about 2.5, or equal to or less than about 3, or greater than 3.
  • the ratio of A to E can be equal to or less than about 2.2, equal to or less than about 2.5, equal to or less than about 2.8, equal to or less than about 3, or greater than 3.
  • Non-elastomeric materials such as glass, steel, brass, copper, silicon, quartz, and hard plastics may be used to form the upper and/or lower layers. These layers may be prepared by machining, etching, casting, molding, embossing, vacuum forming, or similar methodologies. Elastomeric layers may also be prepared and used as described below. Elastomers used in the present invention may have a Young's modulus of from about 1 kilopascal (KPa) to about 2.5 gigapascals (GPa).
  • flow channels preferably have width-to-depth ratios of about 10:1.
  • a non-exclusive list of other ranges of width-to-depth ratios in accordance with embodiments of the present invention is 0.1:1 to 100:1, more preferably 1:1 to 50:1, more preferably 2:1 to 20:1, and most preferably 3:1 to 15:1.
  • flow channels have widths of about 1 to 1000 microns.
  • a non-exclusive list of other ranges of widths of flow channels in accordance with embodiments of the present invention is 0.01 to 1000 microns, more preferably 0.05 to 1000 microns, more preferably 0.2 to 500 microns, more preferably 1 to 250 microns, and most preferably 10 to 200 microns.
  • Elastomeric layers may be cast thick for mechanical stability.
  • a layer is 50 microns to over a centimeter thick, and more preferably approximately 4 mm thick.
  • a non-exclusive list of ranges of thickness of the elastomer layer in accordance with other embodiments of the present invention is between about 0.1 micron to 1 cm, 1 micron to 1 cm, 10 microns to 0.5 cm, 100 microns to 10 mm.
  • membranes separating flow channels have a typical thickness of between about 0.01 and 1000 microns, more preferably 0.05 to 500 microns, more preferably 0.2 to 250, more preferably 1 to 100 microns, more preferably 2 to 50 microns, and most preferably 5 to 40 microns.
  • microfluidic devices disclosed herein are typically constructed at least in part from elastomeric materials and constructed by single and multilayer soft lithography (MSL) techniques and/or sacrificial-layer encapsulation methods (see, e.g., Unger et al. (2000) Science 288:113-116, and PCT Publication WO 01/01025, both of which are incorporated by reference herein in their entirety for all purposes).
  • MSL soft lithography
  • sacrificial-layer encapsulation methods see, e.g., Unger et al. (2000) Science 288:113-116, and PCT Publication WO 01/01025, both of which are incorporated by reference herein in their entirety for all purposes.
  • microfluidic devices can be designed in which solution flow through flow channels of the device is controlled, at least in part, with one or more control channels that are separated from the flow channel by an elastomeric membrane or segment.
  • bonding methods including activating the elastomer surface, for example by plasma exposure, so that the elastomer layers/substrate will bond when placed in contact.
  • activating the elastomer surface for example by plasma exposure
  • elastomer layers/substrate will bond when placed in contact.
  • one possible approach to bonding together elastomer layers composed of the same material is set forth by Duffy et al, "Rapid Prototyping of Microfluidic Systems in Poly (dimethylsiloxane)", Analytical Chemistry (1998), 70, 4974-4984, incorporated herein by reference. This paper discusses that exposing polydimethylsiloxane (PDMS) layers to oxygen plasma causes oxidation of the surface, with irreversible bonding occurring when the two oxidized layers are placed into contact.
  • PDMS polydimethylsiloxane
  • the systems of the present invention may be fabricated from a wide variety of elastomers, hi an exemplary aspect, elastomeric layers may preferably be fabricated from silicone rubber. However, other suitable elastomers may also be used.

Abstract

La présente invention concerne un clapet de non-retour microfluidique robuste ainsi qu'un procédé d'utilisation dudit clapet dans des dispositifs microfluidiques. Le clapet de non-retour est composé de deux chambres disposées l'une au-dessus de l'autre et séparées par une membrane contenant des pores. La membrane est composée d'un matériau élastomère et peut être configurée dans un état normalement ouvert ou normalement fermé. Le clapet de non-retour normalement ouvert peut être mise en œuvre de manière à pouvoir fixer le degré de contre-pression nécessaire pour fermer le clapet. Le mode de réalisation normalement fermé peut maintenir l'état fermé quasiment sans contre-pression. Les deux versions, normalement ouverte ou normalement fermée, sont faciles à fabriquer par des techniques de lithographie molle multicouche et peuvent conserver un fonctionnement efficace après de nombreux milliers de cycles d'ouverture et de fermeture, sans défaillance. De tels clapets de non-retour peuvent remplacer des structures de clapet actives dans des dispositifs microfluidiques et, correctement mis en œuvre, sont susceptibles de simplifier la conception, la fabrication et/ou l'utilisation de dispositifs les contenant.
PCT/US2007/080489 2006-10-04 2007-10-04 Clapets de non-retour microfluidiques WO2008043046A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP07843872.8A EP2074341A4 (fr) 2006-10-04 2007-10-04 Clapets de non-retour microfluidiques

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US84922306P 2006-10-04 2006-10-04
US60/849,223 2006-10-04

Publications (2)

Publication Number Publication Date
WO2008043046A2 true WO2008043046A2 (fr) 2008-04-10
WO2008043046A3 WO2008043046A3 (fr) 2008-08-07

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/080489 WO2008043046A2 (fr) 2006-10-04 2007-10-04 Clapets de non-retour microfluidiques

Country Status (2)

Country Link
EP (1) EP2074341A4 (fr)
WO (1) WO2008043046A2 (fr)

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US8048378B2 (en) 2004-06-07 2011-11-01 Fluidigm Corporation Optical lens system and method for microfluidic devices
US8052792B2 (en) 2001-04-06 2011-11-08 California Institute Of Technology Microfluidic protein crystallography techniques
US8058630B2 (en) 2009-01-16 2011-11-15 Fluidigm Corporation Microfluidic devices and methods
US8105824B2 (en) 2004-01-25 2012-01-31 Fluidigm Corporation Integrated chip carriers with thermocycler interfaces and methods of using the same
US8104515B2 (en) 1999-06-28 2012-01-31 California Institute Of Technology Microfabricated elastomeric valve and pump systems
US8105550B2 (en) 2003-05-20 2012-01-31 Fluidigm Corporation Method and system for microfluidic device and imaging thereof
US8124218B2 (en) 1999-06-28 2012-02-28 California Institute Of Technology Microfabricated elastomeric valve and pump systems
US8157434B2 (en) 2007-01-19 2012-04-17 Fluidigm Corporation High efficiency and high precision microfluidic devices and methods
US8206593B2 (en) 2004-12-03 2012-06-26 Fluidigm Corporation Microfluidic chemical reaction circuits
US8220494B2 (en) 2002-09-25 2012-07-17 California Institute Of Technology Microfluidic large scale integration
US8247178B2 (en) 2003-04-03 2012-08-21 Fluidigm Corporation Thermal reaction device and method for using the same
US8257666B2 (en) 2000-06-05 2012-09-04 California Institute Of Technology Integrated active flux microfluidic devices and methods
US8273574B2 (en) 2000-11-16 2012-09-25 California Institute Of Technology Apparatus and methods for conducting assays and high throughput screening
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US8343442B2 (en) 2001-11-30 2013-01-01 Fluidigm Corporation Microfluidic device and methods of using same
US8388822B2 (en) 1996-09-25 2013-03-05 California Institute Of Technology Method and apparatus for analysis and sorting of polynucleotides based on size
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US8426159B2 (en) 2004-01-16 2013-04-23 California Institute Of Technology Microfluidic chemostat
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US8473216B2 (en) 2006-11-30 2013-06-25 Fluidigm Corporation Method and program for performing baseline correction of amplification curves in a PCR experiment
US8475743B2 (en) 2008-04-11 2013-07-02 Fluidigm Corporation Multilevel microfluidic systems and methods
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US8709153B2 (en) 1999-06-28 2014-04-29 California Institute Of Technology Microfludic protein crystallography techniques
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EP2759751A3 (fr) * 2013-01-29 2016-11-02 Robert Bosch Gmbh Procédé de fabrication d'un clapet anti-retour fermé-normal pour des composants microfluidiques à partir d'un système stratifié polymère et clapet anti-retour
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