WO2007145457A1 - N-terminal modified peg-trail, method for preparing and uses thereof - Google Patents

N-terminal modified peg-trail, method for preparing and uses thereof Download PDF

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Publication number
WO2007145457A1
WO2007145457A1 PCT/KR2007/002825 KR2007002825W WO2007145457A1 WO 2007145457 A1 WO2007145457 A1 WO 2007145457A1 KR 2007002825 W KR2007002825 W KR 2007002825W WO 2007145457 A1 WO2007145457 A1 WO 2007145457A1
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Prior art keywords
trail
peg
terminal modified
conjugate
modified peg
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PCT/KR2007/002825
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French (fr)
Inventor
Kang Choon Lee
Su Young Chae
Yu Seok Youn
Won Bae Kim
Sung Kwon Lee
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Sungkyunkwan University Foundation For Corporate Collaboration
Nexentech Co., Ltd.
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Application filed by Sungkyunkwan University Foundation For Corporate Collaboration, Nexentech Co., Ltd. filed Critical Sungkyunkwan University Foundation For Corporate Collaboration
Priority to EP07746877.5A priority Critical patent/EP2026842B1/en
Priority to ES07746877.5T priority patent/ES2610576T3/en
Priority to DK07746877.5T priority patent/DK2026842T3/en
Priority to US12/304,121 priority patent/US9321825B2/en
Publication of WO2007145457A1 publication Critical patent/WO2007145457A1/en
Priority to US15/138,109 priority patent/US10046059B2/en

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/56—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule
    • A61K47/59—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyureas or polyurethanes
    • A61K47/60—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyureas or polyurethanes the organic macromolecular compound being a polyoxyalkylene oligomer, polymer or dendrimer, e.g. PEG, PPG, PEO or polyglycerol
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/19—Cytokines; Lymphokines; Interferons
    • A61K38/191—Tumor necrosis factors [TNF], e.g. lymphotoxin [LT], i.e. TNF-beta
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/02—Drugs for skeletal disorders for joint disorders, e.g. arthritis, arthrosis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/525—Tumour necrosis factor [TNF]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70575—NGF/TNF-superfamily, e.g. CD70, CD95L, CD153, CD154
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/19—Cytokines; Lymphokines; Interferons
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/73—Fusion polypeptide containing domain for protein-protein interaction containing coiled-coiled motif (leucine zippers)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/96—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation using ion-exchange

Definitions

  • the present invention relates, to an N-terminal modified polyethylene glycol (PEG) -TRAIL conjugate and a preparation method and use thereof. More particularly, the present invention relates to an N-terminal modified PEG-TRAIL conjugate in which PEG or a PEG derivative binds to the N-terminus of TRAIL, a method of preparing the conjugate, and an agent for preventing and treating proliferative diseases or autoimmune diseases comprising the conjugate as an effective ingredient.
  • PEG polyethylene glycol
  • Tumor necrosis factor (TNF) -related apoptosis-inducing ligand is a typical member of the TNF family, and is a membrane protein participating in apoptosis.
  • TRAIL is a protein consisting of 281 amino acids in which an extracellular domain comprising amino acids from arginine at position 114 to glycine at position 281 affects apoptosis.
  • Three molecules of TRAIL form a structurally modified trimer. The TRAIL trimer assembles with receptors participating in cell death to induce apoptosis .
  • TNF and CD95L A major difference between TRA ⁇ L and other members of the TNF superfamily, such as TNF and CD95L, is its ability not to induce cell death at normal tissues.
  • TNF and CD95L A variety of medical or pharmaceutical applications have been attempted on TNF and CD95L, because these proteins induce cell death. Since TNF and CD95L proteins affect normal cells and also induce the death of cancer cells and over-activated immune cells, they have limited applicability.
  • TRAIL induces apoptosis in a wide range of cancer cells and over-activated immune cells with little effect on normal cells. This is due to the differential expression of TRAIL receptors between cell types. Five TRAIL receptors have been identified.
  • DR4 (TRAIL-Rl) and DR5 (TRAIL-R2) are representative cell death-related receptors.
  • TRAIL binds to DR4 or DR5
  • an -intracellular death domain of the receptor is activated and thus transduces apoptotic signals via various signal transduction pathways, leading to apoptotic cell death.
  • TRAIL can also bind to DcRl, DcR2 and osteoprotegerin(OPG) , which do not induce apoptosis. No marked difference has been seen in the expression levels of the cell death-inducing receptors DR4 and DR5 between normal and tumor cells.
  • TRAIL binds mostly to DcRl, DcR2 and OPG, which do not contain a death domain, and thereby do not induce cell death.
  • apoptosis is induced by the binding of TRAIL to DR4 and DR5, which contain a death domain. Such selective apoptosis induction of TRAIL seems to be a particularly attractive feature in medical or pharmaceutical applications .
  • TRAIL-mediated apoptosis has been observed in various types of cancer cells, including colon carcinoma, glioma, lung carcinoma, prostate carcinoma, brain tumors and multiple myeloma cells.
  • TRAIL has been proven to have very high anticancer activity in animals.
  • the good anticancer efficacy of TRAIL has been obtained through the use of TRAIL alone, as well as in combination with other anticancer agents, such as paclitaxel and doxorubicin, and radiotherapy.
  • Clinical trials are currently being conducted by Genentech and Amgen using TRAIL, which has good anticancer efficacy in solid tumors.
  • TRAIL may be useful in the treatment of various aforementioned types of cancer, as well as in the treatment of T cell-mediated autoimmune disorders such as experimental autoimmune encephalomyelitis, rheumatoid arthritis and type I diabetes.
  • native TRAIL has some problems to be overcome for application thereof.
  • the major problem is the low trimer formation ratio of native TRAIL.
  • TRAIL monomers do not bind to the aforementioned TRAIL receptors, and thus do not induce apoptosis.
  • many studies have been performed with the goal of improving the trimeric structure and trimer formation ratio of TRAIL.
  • native TRAIL the zinc ion has been known to play a critical role in trimerization.
  • the structural analysis of TRAIL has been conducted using a computer, and mutants of TRAIL have been developed based on the analysis results.
  • the most useful method appears to be the introduction of a novel amino acid sequence favoring trimeric folding.
  • Such sequences include a leucine zipper motif and an isoleucine zipper motif.
  • Henning Walczak reported the anticancer efficacy of a trimeric TRAIL derivative in which a leucine zipper motif is added to the N terminus of native TRAIL (H. Walczak, et al . Nature Medicine 1999, 5, 157-163) .
  • Dai-Wu Seol et al. reported a TRAIL derivative containing a novel isoleucine zipper motif and having good apoptotic activity (MH Kim, et al. BBRC 2004, 321, 930-935) .
  • Another problem in the clinical applications of TRAIL involves cytotoxicity shown in normal cells of some tissues.
  • TRAIL has a short half-life in vivo, which should be overcome for the successful clinical application of TRAIL.
  • TRAIL has different half-lives according to the species of animals used in tests. For example, TRAIL has been reported to have a half-life of several minutes in rodents and about 30 minutes in apes (H. Xiang, et al. Drug Metabolism and Disposition 2004, 32, 1230- 1238) . In particular, most TRAIL is rapidly excreted via the kidneys. This short half-life is considered a drawback to the pharmaceutical usefulness of TRAIL, resulting in a need for TRAIL or derivatives thereof having an extended half-life. Other problems to be solved include the low solubility and solution stability of TRAIL.
  • polyethylene glycol is a polymer having a structure of HO- (-CH 2 CH 2 O-) n -H. Due to its high hydrophilicity, PEG enables an increase in the solubility of drug proteins when linked thereto.
  • PEG increases the molecular weight of the modified protein while maintaining major biological functions, such as enzyme activity and receptor binding; thereby reducing urinary excretion, protecting the protein from cells and antibodies recognizing exogenous antigens, and decreasing protein degradation by proteases.
  • the molecular weight of PEG, capable of being linked to proteins ranges from about 1,000 to 100,000. PEG having a molecular weight higher than 1,000 is known to have very low toxicity.
  • PEG having a molecular weight between 1,000 and 6,000 is distributed widely throughout the entire body and is metabolized via the kidney.
  • PEG having a molecular weight of 40,000 is distributed in the blood and organs, including the liver, and is metabolized in the liver.
  • US Patent Nos. 4,766,106 and 4,917,888 describes the conjugation of proteins to a polymer including -PEG to increase the water solubility thereof.
  • US Patent No. 4,902,502 describes the conjugation of recombinant proteins to PEG or other polymers to reduce immunogenicity and extend circulating in vivo half-life.
  • PEG is typically conjugated to a target protein through covalent bonding to one or more free lysine (Lys) residues.
  • Lys free lysine
  • the PEG- attached region loses biological functions, leading to decreased protein activity.
  • various kinds of PEG-protein conjugates, corresponding to particular attachment sites exist as a mixture. From this mixture, a desired conjugate is difficult to purify and isolate.
  • TRAIL also harbors problems similar to those caused by pegylation described above.
  • Native TRAIL has eleven lysine residues, some of which participate in the interaction between TRAIL and its cognate receptors or are within an active site. Thus, the addition of polyethylene glycol molecules through the reaction with lysine residues may act as a very important inhibitory factor against the bioactivity of TRAIL. In the case of the TNF superfamily, several studies have noted that the bonding between lysine residues and polyethylene glycol molecules inhibits activation (Y. '' Yamamoto, et al. Nature Biotechnology 2003, 21, 546-552; H. Shibata et al. Clin. Cancer Res. 2004, 10, 8293-8300).
  • the present inventors selectively attached PEG or a PEG derivative to an N-terminus of TRAIL.
  • pegylation was found to reduce drug uptake and removal by hepatocytes and the hepatic reticuloendothelial system, leading to a decrease in TRAIL-mediated hepatoxicity, and remarkably increase the solubility and stability of TRAIL- Also, pegylation was found to improve pharmacokinetic profiles of a linked drug with long-term storage in various formulations, thereby reducing drug administration frequencies and allowing sustained duration of effects of the drug. In this way, the present inventors obtained an N-terminal modified PEG-TRAIL conjugate and found a method of preparing the conjugate, thereby leading to the present invention. [Disclosure]
  • N-terminal modified PEG-TRA 7 IL conjugate or a pharmaceutically acceptable salt thereof. It is another object of the present invention to provide a method of preparing the N-terminal modified PEG-TRAIL conjugate.
  • the present invention provides an N-terminal modified PEG-TRAIL conjugate or a pharmaceutically acceptable salt thereof, a method of preparing the same, and a preventive or therapeutic agent for proliferative diseases or autoimmune diseases comprising the same as an effective ingredient.
  • N-terminal modified PEG-TRAIL conjugate prepared according to the present invention has very high solubility and stability compared to native TRAIL. Also, the pegylated TRAIL has remarkably increased in vivo half-life while retaining biological activity similar to that of native TRAIL. Thus, the pegylated TRAIL may be very useful as a preventive or therapeutic agent for proliferative diseases or autoimmune diseases.
  • FIG. 1 schematically shows the structure of recombinant hTRAIL (ILz-hTRAIL, monomer) for expressing trimeric hTRAIL
  • FIG. 2 is a chromatogram of the recombinant hTRAIL purified through Ni-affinity chromatography (washing 1 (wl) : washing with 10 mM imidazole-containing phosphate buffer; washing 2 (w2) : washing with 50 mM imidazole-containing phosphate buffer)
  • FIG. 3 shows the result of SDS-PAGE of the purified recombinant hTRAIL (EX: E.
  • FIG. 4 shows the result of size exclusion chromatography of products according to PEG to TRAIL reaction ratios
  • FIG. 5 shows the result of size exclusion chromatography of TRAIL (ILz-hTRAIL) , a reaction mixture and a purified N- terminal modified PEG-TRAIL conjugate (PEG5K-ILz-hTRAIL) ;
  • FIG. 6 shows the result of electrophoresis of a reaction mixture, an N-terminal modified PEG-TRAIL conjugate and unreacted TRAIL;
  • FIG. 7 shows the result of a cytotoxicity assay against human cervical carcinoma HeLa cells according to the molecular weight of PEG
  • FIG. 8 is a microscopic photograph showing cell viability according to drug concentrations in the cytotoxicity assay against HeLa cells
  • FIG. 9 shows the result of a cytotoxicity assay against human colon carcinoma HCTl16 cells according to the molecular weight of PEC-
  • FIG. 10 shows the solution solubility of non-pegylated TRAIL and an N-terminal modified PEG-TRAIL conjugate;
  • FIG. 11 shows the pharmacokinetic profiles of non- pegylated TRAIL
  • FIG. 12 shows the pharmacokinetic profiles of an N- terminal modified PEG-TRAIL conjugate.
  • the present invention provides an N- terminal modified PEG-TRAIL conjugate or a pharmaceutically acceptable salt thereof.
  • TRAIL may be obtained in a native or genetically engineered (recombinant) form, and may include a zipper amino acid motif favoring trimer formation or a terminal group facilitating isolation and purification thereof.
  • TRAIL is in the human form, which has an amino acid sequence of 281 amino acids in length, and preferably has an amino acid sequence from arginine-114 to glycine-281 of the full-length human form (1-281) .
  • TRAIL may be attached to an isoleucine zipper at its N- terminus .
  • PEG may include its derivatives .
  • PEG or its derivatives may be a linear or branched form.
  • PEG or its derivatives includes, but is not limited to, methoxy PEG succinimidyl propionate (mPEG-SPA) , methoxy PEG
  • N-hydroxysuccinimide mPEG-NHS
  • mPEG-ALD methoxy PEG aldehyde
  • mPEG-MAL methoxy PEG maleimide
  • PEG may have a molecular weight between 1,000 and 40,000, and preferably between 5,000 and 40,000.
  • the present invention provides a method of preparing the N-terminal modified PEG-TRAIL conjugate.
  • the N-terminal modified PEG-TRAIL conjugate may be obtained by reacting an N-terminal amine of TRAIL with an aldehyde group of PEG in the presence of a reducing agent.
  • PEG may include derivatives thereof.
  • PEG or its derivatives may be in a linear or branched form.
  • PEG or its derivatives includes methoxy PEG succinimidyl propionate (mPEG-SPA) , methoxy PEG N- hydroxysuccinimide (mPEG-NHS) , methoxy PEG aldehyde (rnPEG-ALD) , methoxy PEG maleimide (mPEG-MAL) and multiple-branched polyethylene glycol derivatives.
  • mPEG-SPA methoxy PEG succinimidyl propionate
  • mPEG-NHS methoxy PEG N- hydroxysuccinimide
  • rnPEG-ALD methoxy PEG aldehyde
  • mPEG-MAL methoxy PEG maleimide
  • PEG may have a molecular weight between 1,000 and 40,000, and preferably between 5,000 and 40,000.
  • PEG and TRAIL react at a molar ratio (PEG/TRAIL) of 2 to 10, and preferably 5 to 7.5.
  • TRAIL may be obtained in a native or genetically engineered (recombinant) form.
  • TRAIL may include a zipper amino acid motif favoring trimer formation or a terminal group facilitating isolation and purification thereof.
  • TRAIL is in the human form, which has an amino acid sequence of 281 amino acids in length, and preferably has an amino acid sequence from arginine-114 to glycine-281 of the full-length human form (1-281) .
  • TRAIL may be attached to an isoleucine zipper at its N- terminus .
  • the reducing agent may include NaCNBH 3 and NaBH 4 .
  • the present invention provides a preventive or therapeutic agent for a proliferative disease comprising the N-terminal modified PEG-TRAIL conjugate as an effective ingredient.
  • the proliferative disease is cancer, and preferably includes colon carcinoma, glioma, lung carcinoma, prostate carcinoma, brain tumor and multiple myeloma.
  • the present invention provides a preventive or therapeutic agent for an autoimmune disease comprising the N-terminal modified PEG-TRAIL conjugate as an effective ingredient.
  • the autoimmune disease includes experimental autoimmune encephalomyelitis, rheumatoid arthritis and type I diabetes.
  • the preventive or therapeutic agent comprising the N- terminal modified PEG-TRAIL conjugate as an effective ingredient according to the present invention may be formulated into various formulations for oral or parenteral administration upon clinical application, but is not limited thereto.
  • diluents or excipients may be used, and are exemplified by fillers, thickeners, binders, humectants, disintegrators and surfactants.
  • solid formulations for oral administration include tablets, pills, powders, granules and capsules.
  • the solid formulations may include, in addition to the PEG-TRAIL conjugate, at least one excipient selected from among starch, calcium carbonate, sucrose, lactose, gelatin, etc.
  • the solid formulations may include, in addition to a simple excipient, a lubricant such as magnesium stearate or talc.
  • liquid formulations for oral administration include suspensions, internal solutions, emulsions and syrups.
  • the liquid formulations may include, in addition to commonly used simple diluents such as water and liquid paraffin, various excipients, which are exemplified by humectants, sweeteners, aromatics and preservatives.
  • preparations for parenteral administration include sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations and suppositories.
  • propylene glycol, PEG, vegetable oils such as olive oil, and injectable esters such as ethyl oleate may be used.
  • a formulation may be supplemented with calcium or vitamin D 3 in order to enhance its efficacy as a therapeutic agent for proliferative diseases or autoimmune diseases.
  • the dosage for a specific patient may vary according to the patient's weight, age, gender, state of health and diet, administration duration, administration routes, excretion rates and severity of illness. Typically, it is possible to administer an effective dosage once every one to two weeks. Also, the dosage may be taken in a single dose or in several divided doses within a daily effective dosage. [Mode for Invention]
  • the truncated form of human TRAIL (hTRAIL) used in this test contained amino acids spanning from arginine-114 to glycine-281 of the full-length form (1-281) of hTRAIL.
  • the recombinant TRAIL was expressed and produced in E. coli BL21(DE3), and an expression plasmid pET3a was used.
  • An isoleucine zipper (ILz) favoring trimer folding was added to an N-terminus of the recombinant hTRAIL.
  • the recombinant hTRAIL was then tagged with six histidine ( ⁇ xHis) residues at its N- terminal end in order to facilitate isolation and purification.
  • FIG. 1 schematically shows the structure of the resultant recombinant hTRAIL (monomer) for expressing trimeric hTRAIL (His-tagged and isoleucine zipper-linked) .
  • the transformed E. coli was cultured in sterile LB medium at 37 ° C with agitation for about 12 hrs in the presence of ampicillin (50 mg/L) for selective culture of a transformant .
  • ampicillin 50 mg/L
  • isopropyl- ⁇ -D-thiogalactoside IPTG
  • IPTG isopropyl- ⁇ -D-thiogalactoside
  • 1-2 Purification of the recombinant hTRAIL from E. coli
  • the recombinant hTRAIL was isolated from the transformed E. coli cells harvested in Example 1-1 using a Ni-affinity chromatography. First, the cell pellet obtained by centrifugation was suspended in 20 mM phosphate buffer (pH 7.5), and sonicated to disrupt the cell membrane, thereby releasing TRAIL expressed in E. coli. After insoluble cellular substrates were removed through centrifugation at 10,000 rpm for 20 min, the supernatant was slowly passed through a column pre-packed with Ni-NTA resin. .
  • the recombinant hTRAIL was selectively bound to the column through the interaction between the six N-terminally tagged histidine residues and chelating nickel ions.
  • the column was washed with phosphate buffer (pH 7.5) and phosphate buffer containing 10 mM and 50 mM imidazole to eliminate impurities, including proteins other than the recombinant hTRAIL.
  • the recombinant hTRAIL bound to the column was eluted with phosphate buffer containing 500 mM imidazole.
  • the TRAIL-containing elution fractions were subjected to ultrafiltration to remove the high concentrations of imidazole.
  • the finally isolated, purified product was placed in 50 mM acetate buffer at a pH of 5.0 with a high purity (98% or higher). The results are given in FIG. 2.
  • FIG. 3 shows the result of SDS-PAGE (14% gel) of the isolated and purified recombinant hTRAIL and elution fractions obtained from the Ni-NTA column (EX: E. coli lysates; UB: non- pegylated TRAIL; washing 1 (wl) : washing with 10 mM imidazole- containing phosphate buffer; washing 2 (w2) : washing with 50 mM imidazole-containing phosphate buffer) .
  • the recombinant hTRAIL (ILz-hTRAIL) isolated in Example 1-2 was observed to be eluted at a retention time between 135 and 145 min.
  • the electrophoretic result depicted in FIG. 3 indicated that ILz-hTRAIL was successfully isolated and purified using the Ni-NTA column.
  • 1-3 Synthesis and isolation of PEG-TRAIL conjugates
  • the recombinant hTRAIL prepared in Example 1-2 was diluted at 200 ⁇ g/ml in 50 mM acetate buffer (pH 5.0), and was mixed with methoxy polyethylene glycol 5000 propionaldehyde
  • PEG5k (PEG5k) .
  • a reducing agent NaCNBH 3 was added to the mixture at a final concentration of 20 mM, and the mixture was allowed to react at 4 ° C for about 8 to 12 hrs.
  • PEG5k was added to TRAIL at PEG5k reaction molar ratios of 2.5, 5, 7.5 and 10 in order to prepare an N-terminal modified PEG-TRAIL conjugate.
  • another N-terminal modified PEG-TRAIL conjugate was prepared using methoxy polyethylene glycol 20000 propionaldehyde as described above. The reaction mixtures were subjected to size exclusion chromatography, and the results are given in FIG. 4.
  • the amount of pegylated TRAIL increased with increasing amounts of PEG.
  • excessive PEG reacted with side-chain amines of internal lysine residues as well as a desired N-terminal lysine residue, resulting in increased amounts of byproducts.
  • a resultant conjugate had a higher molecular weight and eluted at an earlier time upon size exclusion chromatography (elution solution: 150 mM NaCl-containing phosphate buffer, pH 6.0).
  • the purified N-terminal modified PEG- TRAIL conjugate was detected at a relatively higher elution volume compared to the non-pegylated recombinant hTRAIL produced in and isolated from E. coli. Also, the chromatogratn indicated that the purified N-terminal modified PEG-TRAIL conjugate was highly pure and did not contain any unreacted TRAIL.
  • FIG. 6 shows the results of electrophoresis of the reaction mixture of this example, the purified recombinant hTRAIL and the purified N-terminal modified PEG-TRAIL conjugate.
  • TRAIL TRAIL monomers were found to exist in the purified N- terminal modified PEG-TRAIL conjugate. This indicates that PEG was bound to the terminal end of one or two monomers of trimeric
  • Non-pegylated TRAIL and the N-terminal modified PEG-TRAIL conjugates were examined for bioactivity, as follows.
  • An apoptosis assay for the non-pegylated recombinant TRAIL and N-terminal modified PEG-TRAIL conjugates was conducted using human cervical carcinoma HeLa cells and human colon carcinoma HCTll ⁇ cells.
  • HeLa cells were cultured in DMEM (Dulbecco's Modified Eagle's Medium), and HCT116 cells in RPMI- 1640. Each cell line was seeded onto a 96-well plate at a density of IxIO 4 cells/well, and was cultured for about 24 hrs to stabilize it.
  • the cells were dosed with control TRAIL (purchased from R&D systems) , the recombinant hTRAIL prepared according to the method of Examples 1-1 and 1-2, and the N- terminal modified PEG-TRAIL conjugates (PEG5K-ILz-hTRAIL and PEG20K-ILz-hTRAIL) at a final concentration of 1 ng/ml to 5 ⁇ g/ml for about 24 hrs. Then, 20 ⁇ l of MTS solution (Promega) was added to each well, and the plate was incubated for about 2 hrs. Absorbance was measured at 490 nm, and cell viability was calculated from the absorbance. The results are given in FIGS. 7, 8 and 9.
  • the N-terminal modified PEG-TRAIL conjugates were found to have cytotoxicity against HeLa cells in a dose-dependent manner.
  • the N-terminal modified PEG-TRAIL conjugates were observed to have relatively low cytotoxicity with increasing molecular weight of the attached PEG.
  • the PEG-TRAIL conjugates exhibited cytotoxicity against colon carcinoma HCTIl6 cells in a manner similar to that observed in FIG. 7. Also, the PEG effect on cytotoxicity against HCT116 cells was similar to that observed against HeLa cells.
  • the non-pegylated recombinant TRAIL and N-terminal modified PEG-TRAIL conjugates were examined for solution stability, as follows.
  • the solubility of non-pegylated and pegylated TRAIL was assayed over time in order to investigate the solution stability thereof.
  • the non-pegylated TRAIL and N-terminal modified PEG-TRAIL conjugates were individually prepared at 200 ⁇ g/ml in 50 mM acetate buffer, diluted at a 1:1 ratio with 100 mM phosphate buffer to adjust the physiological pH value, and then analyzed for solubility over time at 37 ° C. Samples were collected in small amounts at 5, 15, 30, 60, 120 and 180 min. The collected samples were centrifuged to separate soluble and precipitated fractions. For the soluble fractions thus obtained (as supernatants) , protein concentrations were determined using a BCA protein assay kit. The results are given in FIG. 10.
  • the non-pegylated TRAIL (ILz-hTRAIL) and N-terminal modified PEG-TRAIL conjugate (PEG5K-ILz-hTRAIL) were examined for pharmacokinetic profiles, as follows.
  • pharmacokinetic analysis was preformed using 6-week-old Sprague- Dawley rats.
  • the right jugular vein of each of the rats was cannulated by inserting one end of a length of PE-IO tubing into the jugular vein about 2.5 cm deep, and suturing it in place.
  • the other end of the tubing was passed through the back of the animal and connected to a blood collection tube at the exterior.
  • the rats were then allowed to acclimatize and recover for about 24 hrs.
  • the non-pegylated TRAIL and N- terminal modified PEG-TRAIL conjugate were subcutaneously administered to the rats at a dosage of 10 ⁇ g/kg.
  • Blood samples (0.2 ml each) were collected at given time points for a test period of 48 hrs (the first blood sample was collected at 5 min) . After the collected blood samples were centrifuged, the plasma samples were analyzed for in vivo behavior of the non-pegylated TRAIL and N-terminal modified PEG-TRAIL conjugate using a human TRAIL ELISA kit. The results are given in FIGS. 11 and 12, respectively.
  • N-terminal modified PEG-TRAIL conjugate according to the present invention is a pharmacokinetically good drug.
  • compositions comprising the N-terminal modified PEG-TRAIL conjugate according to the present invention were prepared as follows.
  • Lactose 1 g The above components were mixed and placed in an airtight pack, thereby giving powders. 2. Preparation of tablets
  • N-terminal modified PEG-TRAIL conjugate 100 mg Corn starch 100 mg
  • N-terminal modified PEG-TRAIL conjugate 100 mg Corn starch 100 mg
  • Magnesium stearate 2 mg The above components were mixed and loaded into gelatin capsules according to a common capsule preparation method, thereby giving capsules.
  • Injectable sodium chloride BP maximum 1 ml
  • the N-terminal modified PEG-TRAIL conjugate was dissolved in a suitable volume of injectable sodium chloride BP, and was adjusted to a pH of 3.5 using dil-Ute hydrochloric acid BP.
  • Injectable sodium chloride BP was further added to achieve a desired volume, and the solution was sufficiently mixed.
  • the mixture solution was then filled into a 5-ml type I ampule made of transparent glass. The glass was melted to seal the ampule, which was autoclaved at 120 ° C for 15 min, thereby giving an injectable solution.

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Abstract

Disclosed herein are an N-terminal modified PEG-TRAIL conjugate and a preparation method and use thereof. The PEG-TAIL conjugate has pharmaceutical activity identical or similar to that of native TRAIL (TNF-related apoptosis-inducing ligand) with extended in vivo half-life and enhanced stability. Compared to native TRAIL, the PEG- TAIL conjugate exhibits high solubility and solution stability, with highly improved pharmacokinetic profiles. Thus, the PEG-TAIL conjugate may be very useful for preventing and treating proliferative diseases and autoimmune diseases.

Description

[DESCRIPTION]
[invention Title]
N-TEBMINAL MODIFIED PEG-TRAIL, METHOD FOR PREPARING AND USES THEREOF [Technical Field]
The present invention relates, to an N-terminal modified polyethylene glycol (PEG) -TRAIL conjugate and a preparation method and use thereof. More particularly, the present invention relates to an N-terminal modified PEG-TRAIL conjugate in which PEG or a PEG derivative binds to the N-terminus of TRAIL, a method of preparing the conjugate, and an agent for preventing and treating proliferative diseases or autoimmune diseases comprising the conjugate as an effective ingredient.
[Background Art] Tumor necrosis factor (TNF) -related apoptosis-inducing ligand (TRAIL) is a typical member of the TNF family, and is a membrane protein participating in apoptosis. TRAIL is a protein consisting of 281 amino acids in which an extracellular domain comprising amino acids from arginine at position 114 to glycine at position 281 affects apoptosis. Three molecules of TRAIL form a structurally modified trimer. The TRAIL trimer assembles with receptors participating in cell death to induce apoptosis .
A major difference between TRAΪL and other members of the TNF superfamily, such as TNF and CD95L, is its ability not to induce cell death at normal tissues. A variety of medical or pharmaceutical applications have been attempted on TNF and CD95L, because these proteins induce cell death. Since TNF and CD95L proteins affect normal cells and also induce the death of cancer cells and over-activated immune cells, they have limited applicability. In contrast, TRAIL induces apoptosis in a wide range of cancer cells and over-activated immune cells with little effect on normal cells. This is due to the differential expression of TRAIL receptors between cell types. Five TRAIL receptors have been identified. Among them, DR4 (TRAIL-Rl) and DR5 (TRAIL-R2) are representative cell death-related receptors. When TRAIL binds to DR4 or DR5, an -intracellular death domain of the receptor is activated and thus transduces apoptotic signals via various signal transduction pathways, leading to apoptotic cell death. TRAIL can also bind to DcRl, DcR2 and osteoprotegerin(OPG) , which do not induce apoptosis. No marked difference has been seen in the expression levels of the cell death-inducing receptors DR4 and DR5 between normal and tumor cells. In contrast, the three other receptors not inducing apoptosis are expressed at high levels in normal cells, but are either expressed at low levels or are not expressed at all in tumor cells. Thus, in normal cells, TRAIL binds mostly to DcRl, DcR2 and OPG, which do not contain a death domain, and thereby do not induce cell death. In contrast, in cancer cells and over-activated immune cells, apoptosis is induced by the binding of TRAIL to DR4 and DR5, which contain a death domain. Such selective apoptosis induction of TRAIL seems to be a particularly attractive feature in medical or pharmaceutical applications . TRAIL-mediated apoptosis has been observed in various types of cancer cells, including colon carcinoma, glioma, lung carcinoma, prostate carcinoma, brain tumors and multiple myeloma cells. TRAIL has been proven to have very high anticancer activity in animals. The good anticancer efficacy of TRAIL has been obtained through the use of TRAIL alone, as well as in combination with other anticancer agents, such as paclitaxel and doxorubicin, and radiotherapy. Clinical trials are currently being conducted by Genentech and Amgen using TRAIL, which has good anticancer efficacy in solid tumors. In addition to cancer, various approaches using TRAIL have been made in arthritis, an autoimmune disease, for relieving and treating arthropathy by inducing the death of overactivated immune cells. In addition to protein therapy, gene therapy has been attempted through the delivery of the TRAIL gene. Thus, TRAIL may be useful in the treatment of various aforementioned types of cancer, as well as in the treatment of T cell-mediated autoimmune disorders such as experimental autoimmune encephalomyelitis, rheumatoid arthritis and type I diabetes.
However, native TRAIL has some problems to be overcome for application thereof. The major problem is the low trimer formation ratio of native TRAIL. TRAIL monomers do not bind to the aforementioned TRAIL receptors, and thus do not induce apoptosis. In this regard, many studies have been performed with the goal of improving the trimeric structure and trimer formation ratio of TRAIL. In native TRAIL, the zinc ion has been known to play a critical role in trimerization. In addition, the structural analysis of TRAIL has been conducted using a computer, and mutants of TRAIL have been developed based on the analysis results. For the formation of TRAIL trimers, the most useful method appears to be the introduction of a novel amino acid sequence favoring trimeric folding. Such sequences include a leucine zipper motif and an isoleucine zipper motif. Henning Walczak reported the anticancer efficacy of a trimeric TRAIL derivative in which a leucine zipper motif is added to the N terminus of native TRAIL (H. Walczak, et al . Nature Medicine 1999, 5, 157-163) . Dai-Wu Seol et al. reported a TRAIL derivative containing a novel isoleucine zipper motif and having good apoptotic activity (MH Kim, et al. BBRC 2004, 321, 930-935) . Another problem in the clinical applications of TRAIL involves cytotoxicity shown in normal cells of some tissues. Most normal cells are resistant to cytotoxicity, resulting from the expression of the various aforementioned TRAIL receptors, but some hepatocytes and keratinocytes are sensitive to TRAIL- mediated cytotoxicity (H. Yagita, et al. Cancer Sci. 2004, 95, 777-783; M. Jo et al . Nature Medicine 2000, 6, 564-567; S.J. Zheng, et al. J. Clin. Invest. 2004, 113, 58-64).
In addition to toxicity toward some normal cells, TRAIL has a short half-life in vivo, which should be overcome for the successful clinical application of TRAIL. TRAIL has different half-lives according to the species of animals used in tests. For example, TRAIL has been reported to have a half-life of several minutes in rodents and about 30 minutes in apes (H. Xiang, et al. Drug Metabolism and Disposition 2004, 32, 1230- 1238) . In particular, most TRAIL is rapidly excreted via the kidneys. This short half-life is considered a drawback to the pharmaceutical usefulness of TRAIL, resulting in a need for TRAIL or derivatives thereof having an extended half-life. Other problems to be solved include the low solubility and solution stability of TRAIL.
Meanwhile, polyethylene glycol (PEG) is a polymer having a structure of HO- (-CH2CH2O-) n-H. Due to its high hydrophilicity, PEG enables an increase in the solubility of drug proteins when linked thereto. In addition, when suitably linked to a protein, PEG increases the molecular weight of the modified protein while maintaining major biological functions, such as enzyme activity and receptor binding; thereby reducing urinary excretion, protecting the protein from cells and antibodies recognizing exogenous antigens, and decreasing protein degradation by proteases. The molecular weight of PEG, capable of being linked to proteins, ranges from about 1,000 to 100,000. PEG having a molecular weight higher than 1,000 is known to have very low toxicity. PEG having a molecular weight between 1,000 and 6,000 is distributed widely throughout the entire body and is metabolized via the kidney. In particular, PEG having a molecular weight of 40,000 is distributed in the blood and organs, including the liver, and is metabolized in the liver.
In general, medically and pharmaceutically useful proteins administered via parenteral routes are disadvantageous in terms of being immunogenic in the body, being poorly water- soluble and being cleared from circulation within a short period of time. Many studies have been performed to overcome such problems. US Patent No. 4,17-9,337 mentions that, when used as therapeutics, pegylated proteins and enzymes have effects including reduced immunogenicity, increased solubility and extended in vivo residence time, which all are advantages of PEG. After this patent, a variety of efforts have been made to overcome the drawbacks of bioactive proteins through pegylation. An example is Veronese et al. pegylated ribonuclease and superoxide dismutase (Veronese et al., 1985). US Patent Nos. 4,766,106 and 4,917,888 describes the conjugation of proteins to a polymer including -PEG to increase the water solubility thereof. In addition, US Patent No. 4,902,502 describes the conjugation of recombinant proteins to PEG or other polymers to reduce immunogenicity and extend circulating in vivo half-life.
However, despite the aforementioned advantages, there are some problems with protein pegylation, as follows. PEG is typically conjugated to a target protein through covalent bonding to one or more free lysine (Lys) residues. At this time, if PEG is bound to a region directly associated with protein activity among surface regions of the protein, the PEG- attached region loses biological functions, leading to decreased protein activity. Also, since the attachment of PEG to lysine residues mostly occurs in a random manner, various kinds of PEG-protein conjugates, corresponding to particular attachment sites, exist as a mixture. From this mixture, a desired conjugate is difficult to purify and isolate. TRAIL also harbors problems similar to those caused by pegylation described above. Native TRAIL has eleven lysine residues, some of which participate in the interaction between TRAIL and its cognate receptors or are within an active site. Thus, the addition of polyethylene glycol molecules through the reaction with lysine residues may act as a very important inhibitory factor against the bioactivity of TRAIL. In the case of the TNF superfamily, several studies have noted that the bonding between lysine residues and polyethylene glycol molecules inhibits activation (Y. '' Yamamoto, et al. Nature Biotechnology 2003, 21, 546-552; H. Shibata et al. Clin. Cancer Res. 2004, 10, 8293-8300).
In this regard, the present inventors selectively attached PEG or a PEG derivative to an N-terminus of TRAIL.
Such pegylation was found to reduce drug uptake and removal by hepatocytes and the hepatic reticuloendothelial system, leading to a decrease in TRAIL-mediated hepatoxicity, and remarkably increase the solubility and stability of TRAIL- Also, pegylation was found to improve pharmacokinetic profiles of a linked drug with long-term storage in various formulations, thereby reducing drug administration frequencies and allowing sustained duration of effects of the drug. In this way, the present inventors obtained an N-terminal modified PEG-TRAIL conjugate and found a method of preparing the conjugate, thereby leading to the present invention. [Disclosure]
[Technical Problem]
It is therefore an object of the present invention to provide an N-terminal modified PEG-TRA7IL conjugate or a pharmaceutically acceptable salt thereof. It is another object of the present invention to provide a method of preparing the N-terminal modified PEG-TRAIL conjugate.
It is a further object of the present invention to provide the use of the N-terminal modified PEG-TRAIL conjugate. [Technical Solution]
In order to accomplish the above objects, the present invention provides an N-terminal modified PEG-TRAIL conjugate or a pharmaceutically acceptable salt thereof, a method of preparing the same, and a preventive or therapeutic agent for proliferative diseases or autoimmune diseases comprising the same as an effective ingredient. [Advantageous Effects]
An N-terminal modified PEG-TRAIL conjugate prepared according to the present invention has very high solubility and stability compared to native TRAIL. Also, the pegylated TRAIL has remarkably increased in vivo half-life while retaining biological activity similar to that of native TRAIL. Thus, the pegylated TRAIL may be very useful as a preventive or therapeutic agent for proliferative diseases or autoimmune diseases. [Description of Drawings]
FIG. 1 schematically shows the structure of recombinant hTRAIL (ILz-hTRAIL, monomer) for expressing trimeric hTRAIL; FIG. 2 is a chromatogram of the recombinant hTRAIL purified through Ni-affinity chromatography (washing 1 (wl) : washing with 10 mM imidazole-containing phosphate buffer; washing 2 (w2) : washing with 50 mM imidazole-containing phosphate buffer) ; FIG. 3 shows the result of SDS-PAGE of the purified recombinant hTRAIL (EX: E. coli lysates; UB: non-pegylated TRAIL; washing 1 (wl) : washing with 10 inM imidazole-containing phosphate buffer; washing 2 (w2) : washing with 50 inM imidazole- containing phosphate buffer) ; FIG. 4 shows the result of size exclusion chromatography of products according to PEG to TRAIL reaction ratios;
FIG. 5 shows the result of size exclusion chromatography of TRAIL (ILz-hTRAIL) , a reaction mixture and a purified N- terminal modified PEG-TRAIL conjugate (PEG5K-ILz-hTRAIL) ; FIG. 6 shows the result of electrophoresis of a reaction mixture, an N-terminal modified PEG-TRAIL conjugate and unreacted TRAIL;
FIG. 7 shows the result of a cytotoxicity assay against human cervical carcinoma HeLa cells according to the molecular weight of PEG;
FIG. 8 is a microscopic photograph showing cell viability according to drug concentrations in the cytotoxicity assay against HeLa cells;
FIG. 9 shows the result of a cytotoxicity assay against human colon carcinoma HCTl16 cells according to the molecular weight of PEC- FIG. 10 shows the solution solubility of non-pegylated TRAIL and an N-terminal modified PEG-TRAIL conjugate;
FIG. 11 shows the pharmacokinetic profiles of non- pegylated TRAIL; and FIG. 12 shows the pharmacokinetic profiles of an N- terminal modified PEG-TRAIL conjugate. [Best Mode]
In one aspect, the present invention provides an N- terminal modified PEG-TRAIL conjugate or a pharmaceutically acceptable salt thereof.
TRAIL may be obtained in a native or genetically engineered (recombinant) form, and may include a zipper amino acid motif favoring trimer formation or a terminal group facilitating isolation and purification thereof.
TRAIL is in the human form, which has an amino acid sequence of 281 amino acids in length, and preferably has an amino acid sequence from arginine-114 to glycine-281 of the full-length human form (1-281) . TRAIL may be attached to an isoleucine zipper at its N- terminus .
PEG may include its derivatives .
PEG or its derivatives may be a linear or branched form.
Preferably, PEG or its derivatives includes, but is not limited to, methoxy PEG succinimidyl propionate (mPEG-SPA) , methoxy PEG
N-hydroxysuccinimide (mPEG-NHS) , methoxy PEG aldehyde (mPEG-ALD) , methoxy PEG maleimide (mPEG-MAL) and multiple-branched PEG.
PEG may have a molecular weight between 1,000 and 40,000, and preferably between 5,000 and 40,000. In another aspect, the present invention provides a method of preparing the N-terminal modified PEG-TRAIL conjugate.
The N-terminal modified PEG-TRAIL conjugate may be obtained by reacting an N-terminal amine of TRAIL with an aldehyde group of PEG in the presence of a reducing agent.
PEG may include derivatives thereof.
PEG or its derivatives may be in a linear or branched form. Preferably, PEG or its derivatives includes methoxy PEG succinimidyl propionate (mPEG-SPA) , methoxy PEG N- hydroxysuccinimide (mPEG-NHS) , methoxy PEG aldehyde (rnPEG-ALD) , methoxy PEG maleimide (mPEG-MAL) and multiple-branched polyethylene glycol derivatives.
PEG may have a molecular weight between 1,000 and 40,000, and preferably between 5,000 and 40,000. In the present method, PEG and TRAIL react at a molar ratio (PEG/TRAIL) of 2 to 10, and preferably 5 to 7.5.
TRAIL may be obtained in a native or genetically engineered (recombinant) form. Preferably, TRAIL may include a zipper amino acid motif favoring trimer formation or a terminal group facilitating isolation and purification thereof.
TRAIL is in the human form, which has an amino acid sequence of 281 amino acids in length, and preferably has an amino acid sequence from arginine-114 to glycine-281 of the full-length human form (1-281) . TRAIL may be attached to an isoleucine zipper at its N- terminus .
The reducing agent may include NaCNBH3 and NaBH4.
In a further aspect, the present invention provides a preventive or therapeutic agent for a proliferative disease comprising the N-terminal modified PEG-TRAIL conjugate as an effective ingredient.
The proliferative disease is cancer, and preferably includes colon carcinoma, glioma, lung carcinoma, prostate carcinoma, brain tumor and multiple myeloma. In yet another aspect, the present invention provides a preventive or therapeutic agent for an autoimmune disease comprising the N-terminal modified PEG-TRAIL conjugate as an effective ingredient.
The autoimmune disease includes experimental autoimmune encephalomyelitis, rheumatoid arthritis and type I diabetes.
The preventive or therapeutic agent comprising the N- terminal modified PEG-TRAIL conjugate as an effective ingredient according to the present invention may be formulated into various formulations for oral or parenteral administration upon clinical application, but is not limited thereto.
In the formulation, diluents or excipients may be used, and are exemplified by fillers, thickeners, binders, humectants, disintegrators and surfactants. Examples of solid formulations for oral administration include tablets, pills, powders, granules and capsules. The solid formulations may include, in addition to the PEG-TRAIL conjugate, at least one excipient selected from among starch, calcium carbonate, sucrose, lactose, gelatin, etc. Also, the solid formulations may include, in addition to a simple excipient, a lubricant such as magnesium stearate or talc. Examples of liquid formulations for oral administration include suspensions, internal solutions, emulsions and syrups. The liquid formulations may include, in addition to commonly used simple diluents such as water and liquid paraffin, various excipients, which are exemplified by humectants, sweeteners, aromatics and preservatives. Examples of preparations for parenteral administration include sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations and suppositories. In the formulation into non-aqueous solutions and suspensions, propylene glycol, PEG, vegetable oils such as olive oil, and injectable esters such as ethyl oleate may be used. A formulation may be supplemented with calcium or vitamin D3 in order to enhance its efficacy as a therapeutic agent for proliferative diseases or autoimmune diseases. The dosage for a specific patient may vary according to the patient's weight, age, gender, state of health and diet, administration duration, administration routes, excretion rates and severity of illness. Typically, it is possible to administer an effective dosage once every one to two weeks. Also, the dosage may be taken in a single dose or in several divided doses within a daily effective dosage. [Mode for Invention]
A better understanding of the present invention may be obtained through the following examples and test examples, which are set forth to illustrate, but are not to be construed as the limit of the present invention.
Example 1: Preparation of N-terminal modified PEG-TRAIL conjugate
1-1: Culture of E. coli transformed with TFlAIL gene and protein expression
The truncated form of human TRAIL (hTRAIL) used in this test contained amino acids spanning from arginine-114 to glycine-281 of the full-length form (1-281) of hTRAIL. The recombinant TRAIL was expressed and produced in E. coli BL21(DE3), and an expression plasmid pET3a was used. An isoleucine zipper (ILz) favoring trimer folding was added to an N-terminus of the recombinant hTRAIL. The recombinant hTRAIL was then tagged with six histidine (βxHis) residues at its N- terminal end in order to facilitate isolation and purification. FIG. 1 schematically shows the structure of the resultant recombinant hTRAIL (monomer) for expressing trimeric hTRAIL (His-tagged and isoleucine zipper-linked) .
The transformed E. coli was cultured in sterile LB medium at 37°C with agitation for about 12 hrs in the presence of ampicillin (50 mg/L) for selective culture of a transformant . After the transformed E. coli was proliferated for the culturing, isopropyl-β-D-thiogalactoside (IPTG) was added to the culture medium to induce the expression of E. coli genotype. After the agitating incubator and the E. coli culture was reduced to 27°C, 1 ml of IM IPTG solution was added to the culture medium. The cells were further cultured for about 7 hrs with agitation to induce protein expression. The cultured cells were harvested by centrifugation at 5,000 g for 10 min.
1-2: Purification of the recombinant hTRAIL from E. coli The recombinant hTRAIL was isolated from the transformed E. coli cells harvested in Example 1-1 using a Ni-affinity chromatography. First, the cell pellet obtained by centrifugation was suspended in 20 mM phosphate buffer (pH 7.5), and sonicated to disrupt the cell membrane, thereby releasing TRAIL expressed in E. coli. After insoluble cellular substrates were removed through centrifugation at 10,000 rpm for 20 min, the supernatant was slowly passed through a column pre-packed with Ni-NTA resin. . The recombinant hTRAIL was selectively bound to the column through the interaction between the six N-terminally tagged histidine residues and chelating nickel ions. The column was washed with phosphate buffer (pH 7.5) and phosphate buffer containing 10 mM and 50 mM imidazole to eliminate impurities, including proteins other than the recombinant hTRAIL. Then, the recombinant hTRAIL bound to the column was eluted with phosphate buffer containing 500 mM imidazole. The TRAIL-containing elution fractions were subjected to ultrafiltration to remove the high concentrations of imidazole. The finally isolated, purified product was placed in 50 mM acetate buffer at a pH of 5.0 with a high purity (98% or higher). The results are given in FIG. 2.
FIG. 3 shows the result of SDS-PAGE (14% gel) of the isolated and purified recombinant hTRAIL and elution fractions obtained from the Ni-NTA column (EX: E. coli lysates; UB: non- pegylated TRAIL; washing 1 (wl) : washing with 10 mM imidazole- containing phosphate buffer; washing 2 (w2) : washing with 50 mM imidazole-containing phosphate buffer) . As shown in FIG. 2, the recombinant hTRAIL (ILz-hTRAIL) isolated in Example 1-2 was observed to be eluted at a retention time between 135 and 145 min. The electrophoretic result depicted in FIG. 3 indicated that ILz-hTRAIL was successfully isolated and purified using the Ni-NTA column. 1-3: Synthesis and isolation of PEG-TRAIL conjugates
The recombinant hTRAIL prepared in Example 1-2 was diluted at 200 μg/ml in 50 mM acetate buffer (pH 5.0), and was mixed with methoxy polyethylene glycol 5000 propionaldehyde
(PEG5k) . A reducing agent NaCNBH3 was added to the mixture at a final concentration of 20 mM, and the mixture was allowed to react at 4 °C for about 8 to 12 hrs. PEG5k was added to TRAIL at PEG5k reaction molar ratios of 2.5, 5, 7.5 and 10 in order to prepare an N-terminal modified PEG-TRAIL conjugate. Separately, another N-terminal modified PEG-TRAIL conjugate was prepared using methoxy polyethylene glycol 20000 propionaldehyde as described above. The reaction mixtures were subjected to size exclusion chromatography, and the results are given in FIG. 4.
As shown in FIG. 4, the amount of pegylated TRAIL increased with increasing amounts of PEG. However, excessive PEG reacted with side-chain amines of internal lysine residues as well as a desired N-terminal lysine residue, resulting in increased amounts of byproducts. In this case, a resultant conjugate had a higher molecular weight and eluted at an earlier time upon size exclusion chromatography (elution solution: 150 mM NaCl-containing phosphate buffer, pH 6.0). These results indicate that it is effective to set the reaction ratio of PEG to TRAIL within an optimal range so that it is not very high or very low.
An N-terminal modified PEG-TRAIL conjugate, prepared using PEG and TRAIL at a reaction ratio of 7, was isolated and purified through size exclusion chromatography. The results are given in FIG. 5.
As shown in FIG. 5, the purified N-terminal modified PEG- TRAIL conjugate was detected at a relatively higher elution volume compared to the non-pegylated recombinant hTRAIL produced in and isolated from E. coli. Also, the chromatogratn indicated that the purified N-terminal modified PEG-TRAIL conjugate was highly pure and did not contain any unreacted TRAIL.
FIG. 6 shows the results of electrophoresis of the reaction mixture of this example, the purified recombinant hTRAIL and the purified N-terminal modified PEG-TRAIL conjugate.
As shown in FIG. 6, when compared to the band of monomeric
TRAIL, TRAIL monomers were found to exist in the purified N- terminal modified PEG-TRAIL conjugate. This indicates that PEG was bound to the terminal end of one or two monomers of trimeric
TRAIL. Also, on the electrophoresis gel, except for monomeric
TRAIL and monomers of the N-terminal modified PEG-TRAIL conjugate, no impurities were observed, indicating that the finally purified pegylated TRAIL had a high purity.
Experimental Example 1 : Comparison of bioactivity of non- pegylated TRAIL and N-terminal modified PEG-TRAIL conjugates (apoptosis assay)
Non-pegylated TRAIL and the N-terminal modified PEG-TRAIL conjugates were examined for bioactivity, as follows. An apoptosis assay for the non-pegylated recombinant TRAIL and N-terminal modified PEG-TRAIL conjugates was conducted using human cervical carcinoma HeLa cells and human colon carcinoma HCTllβ cells. HeLa cells were cultured in DMEM (Dulbecco's Modified Eagle's Medium), and HCT116 cells in RPMI- 1640. Each cell line was seeded onto a 96-well plate at a density of IxIO4 cells/well, and was cultured for about 24 hrs to stabilize it. The cells were dosed with control TRAIL (purchased from R&D systems) , the recombinant hTRAIL prepared according to the method of Examples 1-1 and 1-2, and the N- terminal modified PEG-TRAIL conjugates (PEG5K-ILz-hTRAIL and PEG20K-ILz-hTRAIL) at a final concentration of 1 ng/ml to 5 μg/ml for about 24 hrs. Then, 20 μl of MTS solution (Promega) was added to each well, and the plate was incubated for about 2 hrs. Absorbance was measured at 490 nm, and cell viability was calculated from the absorbance. The results are given in FIGS. 7, 8 and 9.
As shown in FIGS. 7 and 8, similar to non-pegylated TRAIL, the N-terminal modified PEG-TRAIL conjugates were found to have cytotoxicity against HeLa cells in a dose-dependent manner. The N-terminal modified PEG-TRAIL conjugates were observed to have relatively low cytotoxicity with increasing molecular weight of the attached PEG. These results were consistent with the microscopic results shown in FIG. 8, in which the TRAIL-treated cells were observed microscopically.
As shown in FIG. 9, the PEG-TRAIL conjugates exhibited cytotoxicity against colon carcinoma HCTIl6 cells in a manner similar to that observed in FIG. 7. Also, the PEG effect on cytotoxicity against HCT116 cells was similar to that observed against HeLa cells.
Experimental Example 2: Evaluation of solution solubility of N- terminal modified PEG-TRAIL conjugates
The non-pegylated recombinant TRAIL and N-terminal modified PEG-TRAIL conjugates were examined for solution stability, as follows.
The solubility of non-pegylated and pegylated TRAIL was assayed over time in order to investigate the solution stability thereof. The non-pegylated TRAIL and N-terminal modified PEG-TRAIL conjugates were individually prepared at 200 μg/ml in 50 mM acetate buffer, diluted at a 1:1 ratio with 100 mM phosphate buffer to adjust the physiological pH value, and then analyzed for solubility over time at 37°C. Samples were collected in small amounts at 5, 15, 30, 60, 120 and 180 min. The collected samples were centrifuged to separate soluble and precipitated fractions. For the soluble fractions thus obtained (as supernatants) , protein concentrations were determined using a BCA protein assay kit. The results are given in FIG. 10.
As shown in FIG. 10, a remarkable difference in solution stability was observed between non-pegylated TRAIL and N- terminal modified PEG-TRAIL conjugates. Most non-pegylated TRAIL (more than 80% thereof) was precipitated within 5 min. In contrast, N-terminal modified PEG-TRAIL conjugates exhibited very high solution stability, and this stability increased with increasing molecular weights of PEG. PEG5K-ILz-hTRAIL displayed a stability of about 70%, and PEG20K-ILz-hTRAIL displayed a stability of about 95%.
Experimental Example 3: Evaluation of pharmacokinetic profiles of an N-terminal modified PEG-TRAIL conjugate
The non-pegylated TRAIL (ILz-hTRAIL) and N-terminal modified PEG-TRAIL conjugate (PEG5K-ILz-hTRAIL) were examined for pharmacokinetic profiles, as follows. In order to estimate in vivo behavior of the non-pegylated TRAIL and N-terminal modified PEG-TRAIL conjugate, pharmacokinetic analysis was preformed using 6-week-old Sprague- Dawley rats. In order to facilitate the collection of blood samples from the animal', the right jugular vein of each of the rats was cannulated by inserting one end of a length of PE-IO tubing into the jugular vein about 2.5 cm deep, and suturing it in place. The other end of the tubing was passed through the back of the animal and connected to a blood collection tube at the exterior. The rats were then allowed to acclimatize and recover for about 24 hrs. The non-pegylated TRAIL and N- terminal modified PEG-TRAIL conjugate were subcutaneously administered to the rats at a dosage of 10 μg/kg. Blood samples (0.2 ml each) were collected at given time points for a test period of 48 hrs (the first blood sample was collected at 5 min) . After the collected blood samples were centrifuged, the plasma samples were analyzed for in vivo behavior of the non-pegylated TRAIL and N-terminal modified PEG-TRAIL conjugate using a human TRAIL ELISA kit. The results are given in FIGS. 11 and 12, respectively.
As shown in FIGS. 11 and 12, there was a difference in in vivo behavior between the non-pegylated TRAIL and N-terminal modified PEG-TRAIL conjugate. The non-pegylated TRAIL (ILz- hTRAIL) was rapidly absorbed, and exhibited the highest concentration in the blood after about one hour, but the blood concentration thereof was decreased by rapid renal excretion and metabolization. In contrast, the N-terminal modified PEG- TRAIL conjugate (PEG5K-ILz-hTRAIL) was slowly absorbed from the administered subcutaneous region, and was maintained at high concentrations in the blood for a long period of 48 hrs or longer. This was considered to be due to the low renal excretion and low hepatic metabolization of TRAIL resulting from pegylation. The results of the pharmacokinetic analysis are given in Table 1.
TABLE 1
Pharmacokinetic profiles of non-pegylated TRAIL and N-terminal modified PEG-TRAIL conjugate
Figure imgf000026_0001
As shown in Table 1, compared to ILz-hTRAIL, PEG5K-ILz- hTRAIL existed at higher levels in blood, and had Tm3x (time to reach maximum concentration) more than 19 times higher and Ti/230 times longer. These results indicate that the N-terminal modified PEG-TRAIL conjugate according to the present invention is a pharmacokinetically good drug.
Hereinafter, exemplary formulations of the present PEG- TRAIL conjugate will be given.
Formulation Examples: Preparation of pharmaceutical formulations
Pharmaceutical formulations comprising the N-terminal modified PEG-TRAIL conjugate according to the present invention were prepared as follows.
1. Preparation of powders
N-terminal modified PEG-TRAIL conjugate 2 g
Lactose 1 g The above components were mixed and placed in an airtight pack, thereby giving powders. 2. Preparation of tablets
N-terminal modified PEG-TRAIL conjugate 100 mg Corn starch 100 mg
Lactose 100 mg
Magnesium stearate 2 mg
The above components were mixed and tabletted according to a common tablet preparation method, thereby giving tablets . 3. Preparation of capsules '
N-terminal modified PEG-TRAIL conjugate 100 mg Corn starch 100 mg
Lactose 100 mg
Magnesium stearate 2 mg The above components were mixed and loaded into gelatin capsules according to a common capsule preparation method, thereby giving capsules.
4. Preparation of injectable solution N-terminal modified PEG-TRAIL conjugate 10 μg/ml Dilute hydrochloric acid BP up to pH 3,, 5
Injectable sodium chloride BP maximum 1 ml The N-terminal modified PEG-TRAIL conjugate was dissolved in a suitable volume of injectable sodium chloride BP, and was adjusted to a pH of 3.5 using dil-Ute hydrochloric acid BP. Injectable sodium chloride BP was further added to achieve a desired volume, and the solution was sufficiently mixed. The mixture solution was then filled into a 5-ml type I ampule made of transparent glass. The glass was melted to seal the ampule, which was autoclaved at 120°C for 15 min, thereby giving an injectable solution.

Claims

[CLAIMS]
[Claim l]
An N-terminal modified polyethylene glycol (PEG) -tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) con j ugate .
[Claim 2]
The N-terminal modified PEG-TRAIL conjugate as set forth in claim 1, wherein the PEG comprises a derivative thereof.
[Claim 3] The N-terminal modified PEG-TRAIL conjugate according to claim 1 or 2, wherein the PEG or derivative thereof has a linear or branched form.
[Claim 4]
The N-terminal modified PEG-TRAIL conjugate according to claim 1, wherein the PEG is at least one selected from the group consisting of methoxypolyethylene glycol succinimidyl propionate, methoxypolyethylene glycol N-hydroxysuccinimide, methoxypolyethylene glycol aldehyde, methoxypolyethylene glycol maleimide and multiple-branched polyethylene glycol.
[Claim 5]
The N-terminal modified PEG-TRAIL conjugate according to claim 4 , wherein the PEG is methoxypolyethylene glycol aldehyde .
[Claim 6]
The N-terminal modified PEG-TRAIL conjugate according to claim 1, wherein the PEG has a molecular weight between 1,000 and 40,000.
[Claim 7]
The N-terminal modified PEG-TRAIL conjugate according to claim 6, wherein the PEG has a molecular weight between 5,000 and 40,000.
[Claim 8]
The N-terminal modified PEG-TRAIL conjugate according to claim 1, wherein the TRAIL is obtained in a native or genetically engineered (recombinant) form.
[Claim 9]
The N-terminal modified PEG-TRAIL conjugate according to claim 8, wherein the TRAIL comprises a zipper amino acid motif favoring trimer formation or a terminal group facilitating isolation and purification.
[Claim 10] The N-terminal modified PEG-TRAIL conjugate according to claim 1, wherein the TRAIL is human TRAIL, having an amino acid sequence 281 amino acids in length.
[Claim 11] The N-terminal modified PEG-TRAIL conjugate according to claim 10, wherein the TRAIL has an amino acid sequence from arginine-114 to glycine-281 of the full-length human form (1- 281) .
[Claim 12] The N-terminal modified PEG-TRAIL conjugate according to claim 1, wherein the TRAIL is attached to an isoleucine zipper at an N-terminus thereof.
[Claim 13]
A method of preparing the N-terminal modified PEG-TRAIL conjugate of claim 1, comprising reacting an N-terminal amine of TRAIL with an aldehyde group of PEG in presence of a reducing agent.
[Claim 14]
The method according to claim 13, wherein the PEG comprises a derivative thereof.
[Claim 15]
The method according to claim 13, wherein the PEG or derivative thereof has a linear or branched form.
[Claim 16] The method according to claim 13, wherein the PEG is at least one selected from the group consisting of methoxypolyethylene glycol succinimidyl propionate, methoxypolyethylene glycol N-hydroxysuccinimide, methoxypolyethylene glycol aldehyde, methoxypolyethylene glycol maleimide and multiple-branched polyethylene glycol.
[Claim 17]
The method according to claim 13, wherein the PEG has a molecular weight between 1,000 and 40,000.
[Claim 18] The method according to claim 17, wherein the PEG has a molecular weight between 5,000 and 40,000.
[Claim 19]
The method according to claim 13, wherein the PEG and TRAIL react at a molar ratio (PEG/TRAIL) of 2 to 10.
[Claim 20] The method according to claim 13, wherein the PEG and TRAIL react at a molar ratio (PEG/TRAIL) of 5 to 7.5.
[Claim 21]
The method according to claim 13, wherein the TRAIL is obtained in a native or genetically engineered (recombinant) form.
[Claim 22]
The method according to claim 21, wherein the TRAIL comprises a zipper amino acid motif favoring trimer formation or a terminal group facilitating isolation and purification.
[Claim 23]
The method according to claim 21, wherein the TRAIL is human TRAIL, having an amino acid sequence 281 amino acids in length.
[Claim 24]
The method according to claim 23, wherein the TRAIL has an amino acid sequence from arginine-114 to glycine-281 of the full-length human form (1-281) .
[Claim 25] The method according to claim 24, wherein the TRAIL is attached to an isoleucine zipper at an N-terminus thereof.
[Claim 26]
The method according to claim 13, wherein the reducing agent is NaCNBH3 or NaBH4.
[Claim 27]
A preventive or therapeutic agent for a proliferative disease comprising the N-terminal modified PEG-TRAIL conjugate of claim 1 as an effective ingredient.
[Claim 28] The preventive or therapeutic agent according to claim 27, wherein the proliferative disease is cancer.
[Claim 29]
The preventive or therapeutic agent according to claim 28, wherein the cancer is one selected from among colon carcinoma, glioma, lung carcinoma, prostate carcinoma, brain tumor and multiple myeloma.
[Claim 30]
A preventive or therapeutic agent for an autoimmune disease, comprising the N-terminal modified PEG-TRAIL conjugate of claim 1 as an effective ingredient. [Claim 31]
The preventive or therapeutic agent according to claim 30, wherein the autoimmune disease is one selected from among experimental autoimmune encephalomyelitis, rheumatoid arthritis and type I diabetes.
PCT/KR2007/002825 2006-06-12 2007-06-12 N-terminal modified peg-trail, method for preparing and uses thereof WO2007145457A1 (en)

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