WO2007117258A3 - Growth of wild-type hepatitis a virus in cell culture - Google Patents

Growth of wild-type hepatitis a virus in cell culture Download PDF

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Publication number
WO2007117258A3
WO2007117258A3 PCT/US2006/024748 US2006024748W WO2007117258A3 WO 2007117258 A3 WO2007117258 A3 WO 2007117258A3 US 2006024748 W US2006024748 W US 2006024748W WO 2007117258 A3 WO2007117258 A3 WO 2007117258A3
Authority
WO
WIPO (PCT)
Prior art keywords
hav
growth
nucleic acid
virus
replication
Prior art date
Application number
PCT/US2006/024748
Other languages
French (fr)
Other versions
WO2007117258A9 (en
WO2007117258A2 (en
Inventor
Gerardo Kaplan
Krishnamurthy Konduru
Original Assignee
Us Gov Health & Human Serv
Gerardo Kaplan
Krishnamurthy Konduru
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Us Gov Health & Human Serv, Gerardo Kaplan, Krishnamurthy Konduru filed Critical Us Gov Health & Human Serv
Priority to US11/993,851 priority Critical patent/US20100055670A1/en
Priority to EP06851110A priority patent/EP1915454A2/en
Priority to CA002613827A priority patent/CA2613827A1/en
Publication of WO2007117258A2 publication Critical patent/WO2007117258A2/en
Publication of WO2007117258A9 publication Critical patent/WO2007117258A9/en
Publication of WO2007117258A3 publication Critical patent/WO2007117258A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/32011—Picornaviridae
    • C12N2770/32411—Hepatovirus, i.e. hepatitis A virus
    • C12N2770/32441—Use of virus, viral particle or viral elements as a vector
    • C12N2770/32443—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/32011—Picornaviridae
    • C12N2770/32411—Hepatovirus, i.e. hepatitis A virus
    • C12N2770/32461—Methods of inactivation or attenuation

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

The invention provides recombinant Hepatitis A Virus (HAV) nucleic acids and host cells that are permissive for their growth and replication. The recombinant Hepatitis A Virus nucleic acids not particularly limited, except that they incorporate at least one heterologous nucleic acid fragment. The heterologous nucleic acid can encode a selectable marker gene and such recombinant HAV nucleic acids are useful for selecting cells that are permissive for growth and replication of wild type HAV. Alternatively, the heterologous nucleic acid may encode a vaccine antigen or other expression product that is desirable to express in a cell harboring the recombinant HAV nucleic acid. The invention further provides cell lines permissive for growth and replication of wild type HAV or HAV having minimal mutations for growth in cell culture. The invention further provides methods for producing HAV vaccines and for monitoring environmental and patient samples for the presence of HAV.
PCT/US2006/024748 2005-06-28 2006-06-26 Growth of wild-type hepatitis a virus in cell culture WO2007117258A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US11/993,851 US20100055670A1 (en) 2005-06-28 2006-06-26 Growth of wild-type hepatitis a virus in cell culture
EP06851110A EP1915454A2 (en) 2005-06-28 2006-06-26 Growth of wild-type hepatitis a virus in cell culture
CA002613827A CA2613827A1 (en) 2005-06-28 2006-06-26 Growth of wild-type hepatitis a virus in cell culture

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US69423205P 2005-06-28 2005-06-28
US60/694,232 2005-06-28

Publications (3)

Publication Number Publication Date
WO2007117258A2 WO2007117258A2 (en) 2007-10-18
WO2007117258A9 WO2007117258A9 (en) 2008-04-17
WO2007117258A3 true WO2007117258A3 (en) 2008-06-12

Family

ID=38581502

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/024748 WO2007117258A2 (en) 2005-06-28 2006-06-26 Growth of wild-type hepatitis a virus in cell culture

Country Status (5)

Country Link
US (1) US20100055670A1 (en)
EP (1) EP1915454A2 (en)
CN (1) CN101356280A (en)
CA (1) CA2613827A1 (en)
WO (1) WO2007117258A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2392445T3 (en) 2004-07-13 2012-12-10 Gen-Probe Incorporated Compositions and methods for the detection of hepatitis A virus nucleic acid
CN108841926B (en) * 2018-07-13 2021-10-01 锦州医科大学 Primer, probe and kit for dual detection of hepatitis E virus and hepatitis A virus by RT-RPA-lateral flow chromatography
WO2020146598A1 (en) * 2019-01-09 2020-07-16 Emv Enhance (Hk) Limited Compositions and methods for enhancing immune response to vaccination and improving vaccine production
KR102277089B1 (en) * 2019-12-19 2021-07-14 에스케이바이오사이언스(주) Method for Preparing Hepatitis A Virus and Hepatitis A Virus Prepared by the Method

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG73999A1 (en) * 1992-09-18 2000-07-18 Us Health Hepatitis a virus vaccines

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
BEARD MICHAEL R ET AL: "Characterization of recombinant hepatitis A virus genomes containing exogenous sequences at the 2A/2B junction", JOURNAL OF VIROLOGY, vol. 75, no. 3, February 2001 (2001-02-01), pages 1414 - 1426, XP002470095, ISSN: 0022-538X *
BENEDUCE FRANCESCA ET AL: "Chimeric hepatitis A virus particles presenting a foreign epitope (HIV gp41) at their surface", ANTIVIRAL RESEARCH, vol. 55, no. 2, August 2002 (2002-08-01), pages 369 - 377, XP002470096, ISSN: 0166-3542 *
BLIGHT KERIL J ET AL: "Highly permissive cell lines for subgenomic and genomic hepatitis C virus RNA replication", JOURNAL OF VIROLOGY, THE AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 76, no. 24, December 2002 (2002-12-01), pages 13001 - 13014, XP002426792, ISSN: 0022-538X *
GRAFF JUDITH ET AL: "Nucleotide sequence of wild-type hepatitis A virus GBM in comparison with two cell culture-adapted variants", JOURNAL OF VIROLOGY, vol. 68, no. 1, 1994, pages 548 - 554, XP002470097, ISSN: 0022-538X *
KONDURU KRISHNAMURTHY ET AL: "Stable growth of wild-type hepatitis A virus in cell culture", JOURNAL OF VIROLOGY, vol. 80, no. 3, February 2006 (2006-02-01), pages 1352 - 1360, XP002470094, ISSN: 0022-538X *

Also Published As

Publication number Publication date
US20100055670A1 (en) 2010-03-04
CN101356280A (en) 2009-01-28
EP1915454A2 (en) 2008-04-30
WO2007117258A9 (en) 2008-04-17
WO2007117258A2 (en) 2007-10-18
CA2613827A1 (en) 2007-10-18

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