WO2007107758A1

WO2007107758A1 - Antibacterial agents

Info

Publication number: WO2007107758A1
Application number: PCT/GB2007/001012
Authority: WO
Inventors: David Ryall Brown; Ian Collins; Lloyd George Czaplewski; David John Hayden
Original assignee: Prolysis Ltd
Priority date: 2006-03-23
Filing date: 2007-03-22
Publication date: 2007-09-27
Also published as: AU2007228539A1; PL1996180T3; DK1996180T3; US8088791B2; NZ571271A; ATE518564T1; MY148430A; EA015198B1; AU2007228539B2; MX2008011857A; US8865736B2; US20130072520A2; JP2009531322A; CA2648785A1; CN102008459A; EP1996180B1; EA200802036A1; EP1996180A1; CN102008459B; KR20090017483A

Abstract

Compounds of formula (I) have antibacterial activity wherein R represents hydrogen or 1, 2 or 3 optional substituents; W is =C(R1)- or =N-; R1 is hydrogen or an optional substituent and R2 is hydrogen, methyl, or fluorine; or R1 and R2 taken together are -CH2-, -CH2CH2-, -O-, or, in either orientation, -O- CH2- Or -OCH2CH2-; R3 is a radical of formula -(Alk1)m-(Z)p-(Alk2)n-Q wherein m, p and n are independently 0 or 1, provided that at least one of m, p and n is 1, Z is -O-, -S-, -S(O)-, -S(O2)-, -NH-, -N(CH3)-, -N(CH2CH3)-, -C(=O)-, -O-(C=O)-, -C(=O)-O-, or an optionally substituted divalent monocyclic carbocyclic or heterocyclic radical having 3 to 6 ring atoms; or an optionally substituted divalent bicyclic heterocyclic radical having 5 to 10 ring atoms; Alk1 and Alk2 are optionally substituted C1C6 alkylene, C2-C6 alkenylene, or C2-C6 alkynylene radicals, which may optionally terminate with or be interrupted by -O-, -S-, -S(O)-, -S(O2)-, -NH-, -N(CH3)-, or -N(CH2CH3)-; and Q is hydrogen, halogen, nitrile, or hydroxyl or an optionally substituted monocyclic carbocyclic or heterocyclic radical having 3 to 6 ring atoms; or an optionally substituted bicyclic heterocyclic radical having 5 to 10 ring atoms.

Description

Antibacterial Agents

This invention relates to the use of a class of substituted benzamides and pyridylamides as antibacterial agents, to novel members of that class perse, and to pharmaceutical compositions comprising such compounds.

Background to the Invention

Many classes of antibacterial agents are known, including the penicillins and cephalosporins, tetracyclines, sulfonamides, monobactams, fluoroquinolones and quinolones, aminoglycosides, glycopeptides, macrolides, polymyxins, lincosamides, trimethoprim and chloramphenicol. The fundamental mechanisms of action of these antibacterial classes vary.

Bacterial resistance to many known antibacterials is a growing problem. Accordingly there is a continuing need in the art for alternative antibacterial agents, especially those that have mechanisms of action fundamentally different from the known classes.

Amongst the Gram-positive pathogens, such as staphylococci, streptococci, mycobacteria and enterococci, resistant strains have evolved/arisen which make them particularly difficult to eradicate. Examples of such strains are methicillin resistant Staphylococcus aureus (MRSA), methicillin resistant coagulase negative staphylococci (MRCNS), penicillin resistant Streptococcus pneumoniae and multiply resistant Enterococcus faecium. In view of the rapid emergence of multidrug-resistant bacteria, the development of antibacterial agents with novel modes of action that are effective against the growing number of resistant bacteria, particularly the vancomycin resistant enterococci and beta-lactam antibiotic-resistant bacteria, such as methicillin-resistant Staphylococcus aureus, is of utmost importance.

Cell division has been of considerable interest to the pharmaceutical industry as a target because it comprises a group of well conserved target proteins that are all essential for the viability of a wide range of bacteria, and their activities are completely different from those of the proteins involved in cell division of mammalian cells. A number of compounds that act on components of the cell division machinery have been described (Ohashi, Y. et a/. J. Bacteriol. 181 , 1348-1351 (1999),

Jennings, L. D. et al. Bioorg Med Chem 12, 5115-5131 (2004), Sutherland, A.G. et al. Org Biomol Chem 1 , 4138-4140 (2003), Margalit, D.N. et al. Proc. Natl. Acad. Sci. USA 101 , 11821-11826 (2004), Wang, J. et a/. J. Biol. Chem. 278, 44424-44428 (2003), White, E.L. et a/. J. Antimicrob. Chemother. 50, 111-114 (2002), Reynolds, R.C. et al. Bioorg Med Chem Lett 14, 3161-3164 (2004) and Stokes et al. J Biol Chem. 280, 39709-39715 (2005)). So far, most effort has been directed at the FtsZ protein, since it has several biochemical activities that can be assayed in vitro. Unfortunately, most of the compounds described so far either have relatively low potency, undesirable pharmacological properties or unknown specificity.

Brief Description of the Invention

This invention is based on the finding that a class of substituted benzamides and pyridylamides has antibacterial activity as evidenced by inhibition of bacterial growth by members of that class. The compounds exhibit activity against strains of Gram- positive bacteria, such as staphylococci, Clostridia, listeria and bacilli, for example Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus haemolyticus and Staphylococcus saprophytics, Bacillus subtilis, Bacillus anthracis and Bacillus cereus. Whilst the invention is not limited by any particular hypothesis as to the mechanism of action of the compounds, it is presently believed that such activity is mediated by the compounds inhibiting cell division through binding to FtsZ.

Detailed Description of the Invention

According to a broad aspect of the invention, there is provided the use of a compound which is a substituted benzamide or pyridylamide of formula (I) or a salt, hydrate, or solvate thereof, in the manufacture of a medicament for use in treating bacterial infection:

wherein

R represents hydrogen or 1, 2 or 3 optional substituents;

W is ^S=C(R₁)- or =N-; Ri is hydrogen or an optional substituent and R₂ is methyl, hydrogen or fluorine; or R₁ and R₂ taken together are -CH₂-, -CH₂CH₂-, -O-, or, in either orientation, -0-CH₂-, - OCH₂CH₂-;

R₃ is a radical of formula -(Alk¹)_m-(Z)_p-(Alk²)_n-Q wherein

m, p and n are independently 0 or 1, provided that at least one of m, p and n is 1.

Z is -O-, -S-, -S(O)-, -S(O₂)-, -NH-, -N(CH₃)-, -N(CH₂CH₃)-, -C(=0)-, -0-,

-(C=O)-, -C(=O)-O-, or an optionally substituted divalent monocyclic carbocyclic or heterocyclic radical having 3 to 6 ring atoms; or an optionally substituted divalent bicyclic heterocyclic radical having 5 to 10 ring atoms;

AIk¹ and AIk² are optionally substituted C₁-C₆ alkylene, C₂-C₆ alkenylene, or C₂-C₆ alkynylene radicals, which may optionally terminate with or be interrupted by -0-, -S-, -S(O)-, -S(O₂)-, -NH-, -N(CH₃)-, or -N(CH₂CH₃)-; and

Q is hydrogen, halogen, nitrile (-CN), or hydroxy! or an optionally substituted monocyclic carbocyclic or heterocyclic radical having 3 to 7 ring atoms; or an optionally substituted bicyclic heterocyclic radical having 5 to 10 ring atoms.

In other broad aspects, the invention includes (i) a method of treating bacterial infection in a subject suffering such infection comprising administering to the subject an amount of a compound (I) as defined above, sufficient to inhibit bacterial growth;

(ii) a method of treating bacterial contamination of a substrate comprising applying to the site of such contamination an amount of a compound (I) as defined above, sufficient to inhibit bacterial growth;

(iii) a compound (I) as defined above for use in a method of treatment of the human body;

(iv) a compound (I) as defined above for use in treating bacterial infection;

Some members of the class of compounds defined by formula (I) above are believed novel in their own right, and the invention includes all such novel members of the class. Thus the invention also includes novel compounds which are substituted benzamides or pyridylamides of formula (IC) and salts, hydrates or solvates thereof:

wherein W is =C(Ri)- or =N-; Ri is hydrogen or an optional substituent and R₂ is hydrogen, methyl, or fluoro; or R₁ and R₂ taken together are -CH₂-, -CH₂CH₂-, -O- or, in either orientation, -O-CH₂-or -OCH₂CH₂-; R₄ and R₅ are independently fluoro or chloro, or one of R₄ and R₅ is hydrogen while the other is fluoro or chloro; and R₃ is a radical selected from those of the following formulae A-H, in which any vacant ring position is optionally substituted:

A B C

D E F

wherein Q is hydrogen, halogen, nitrile, or hydroxyl; or an optionally substituted monocyclic carbocyclic or heterocyclic radical having 3 to 6 ring atoms; or an optionally substituted bicyclic heterocyclic radical having 5 to 10 ring atoms.

The invention also includes novel pyridylamide compounds of formula (ID) and salts, hydrates or solvates thereof:

wherein R₂ is hydrogen, methyl, or fluoro; and R₃ is as defined in relation to formula (IC).

Terminology

As used herein, the term "(C_a-C_b)alkyl" wherein a and b are integers refers to a straight or branched chain alkyl radical having from a to b carbon atoms. Thus when a is 1 and b is 6, for example, the term includes methyl, ethyl, n-propyl, isopropyl, n- butyl, isobutyl, sec-butyl, t-butyl, n-pentyl and n-hexyl.

As used herein the term "divalent (C_a-C_b)alkylene radical" wherein a and b are integers refers to a saturated hydrocarbon chain having from a to b carbon atoms and two unsatisfied valences. The term includes, for example, methylene, ethylene, n-propylene and n-butylene.

As used herein the term "(C_a-C_b)alkenyl" wherein a and b are integers refers to a straight or branched chain alkenyl moiety having from a to b carbon atoms having at least one double bond of either E or Z stereochemistry where applicable. The term includes, for example, vinyl, allyl, 1- and 2-butenyl and 2-methyl-2-propenyl.

As used herein the term "divalent (C_a-C_b)alkenylene radical" means a hydrocarbon chain having from a to b carbon atoms, at least one double bond, and two unsatisfied valences. The term includes, for example, -CH=CH- (vinylene), -CH=CH-CH₂-, -CH₂- CH=CH-, -CH=CH-CH₂-CH₂-, -CH=CH-CH₂-CH₂-CH₂-, -CH=CH-CH=CH-, -CH=CH-CH=CH-CH₂-, -CH=CH-CH=CH-CH₂-CH₂-, -CH=CH-CH₂-CH=CH-, and -CH=CH-CH₂-CH₂-CH=CH-.

As used herein the term "C_a-C_b alkynyl" wherein a and b are integers refers to straight chain or branched chain hydrocarbon groups having from a to b carbon atoms and having in addition at least one triple bond. This term would include for example, ethynyl, 1-propynyl, 1- and 2-butynyl, 2-methyl-2-propynyl, 2-pentynyl, 3- pentynyl, 4-pentynyl, 2-hexynyl, 3-hexynyl, 4-hexynyl and 5-hexynyl.

As used herein the term "divalent (C_a-C_b)alkynylene radical" wherein a and b are integers refers to a divalent hydrocarbon chain having from a to b carbon atoms, and at least one triple bond. The term includes, for example, -C≡C-, -C≡C-CH₂-, and -CH₂-C=CH-.

As used herein the term "cycloalkyl" refers to a monocyclic or bridged monocyclic saturated carbocyclic radical having from 3-8 carbon atoms and includes, for example, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclooctyl and bicyclo[2.2.1]hept-1-yl.

As used herein the unqualified term "aryl" refers to a mono- or bi-cyclic carbocyclic aromatic radical. Illustrative of such radicals are phenyl and naphthyl.

As used herein the unqualified term "heteroaryl" refers to a mono-, or bi-cyclic aromatic radical containing one or more heteroatoms selected from S, N and O, and includes radicals having two such monocyclic rings, or one such monocyclic ring and one monocyclic aryl ring, which are fused or directly linked by a covalent bond. Illustrative of such radicals are thienyl, benzthienyl, furyl, benzfuryl, pyrrolyl, imidazolyl, benzimidazolyl, thiazolyl, benzthiazolyl, thiazolopyridinyl, isothiazolyl, benzisothiazolyl, pyrazolyl, oxazolyl, benzoxazolyl, isoxazolyl, benzisoxazolyl, isothiazolyl, triazolyl, benztriazolyl, thiadiazolyl, oxadiazolyl, pyridinyl, pyridazinyl, pyrimidinyl, pyridazinyl, triazinyl, indolyl and indazolyl.

As used herein the unqualified term "heterocyclyl" or "heterocyclic" includes "heteroaryl" as defined above, and in addition means a mono-, or bi-cyclic non- aromatic radical containing one or more heteroatoms selected from S, N and O. Illustrative of such radicals are pyrrolyl, furanyl, thienyl, piperidinyl, imidazolyl, oxazolyl, isoxazolyl, thiazolyl, thiadiazolyl, pyrazolyl, pyridinyl, pyrrolidinyl, pyrimidinyl, morpholinyl, piperazinyl, indolyl, morpholinyl, benzfuranyl, pyranyl, isoxazolyl, benzimidazolyl, methylenedioxyphenyl, ethylenedioxyphenyl, maleimido and succinimido groups. Unless otherwise specified in the context in which it occurs, the term "substituted" as applied to any moiety herein means substituted with up to four compatible substituents, each of which independently may be, for example, (Ci-C₆)alkyl, (C₂- C₆)alkenyl, (C₂-C₆)alkynyl, (C₁-C₆JaIkOXy, hydroxy, hydroxy(C₁-C₆)alkyl, mercapto, mercapto^-CeJalkyl, (Ci-C₆)alkylthio, halo (including fluoro, bromo and chloro), fully or partially fluorinated (CrC₃)alkyl, (C_rC₃)alkoxy or (Ci-Csjalkylthio such as trifluoromethyl, trifluoromethoxy, and trifluoromethylthio, nitro, nitrile (-CN), oxo (=O), phenyl, phenoxy, monocyclic heteroaryl or heteroaryloxy with 5 or 6 ring atoms, -COOR^A, -COR^A, -OCOR^A, -SO₂R^A, -CONR^AR^B, -SO₂NR^AR^B, -NR^AR^B, OCONR^AR^B, -NR^BCOR^A, -NR^BCOOR^A, -NR^BSO₂OR^A or -NR^ACONR^AR^B wherein R^A and R^B are independently hydrogen or a (Ci-C₆)alkyl group or, in the case where R^A and R^B are linked to the same N atom, R^A and R^B taken together with that nitrogen may form a cyclic amino ring. Where the substituent is phenyl, phenoxy or monocyclic heteroaryl or heteroaryloxy with 5 or 6 ring atoms, the phenyl or heteroaryl ring thereof may itself be substituted by any of the above substituents except phenyl phenoxy, heteroaryl or heteroaryloxy. An "optional substituent" or "substituent" may be one of the foregoing specified groups.

As used herein the term "salt" includes base addition, acid addition and quaternary salts. Compounds of the invention which are acidic can form salts, including pharmaceutically acceptable salts, with bases such as alkali metal hydroxides, e.g. sodium and potassium hydroxides; alkaline earth metal hydroxides e.g. calcium, barium and magnesium hydroxides; with organic bases e.g. N-methyl-D-glucamine, choline tris(hydroxymethyl)amino-methane, L-arginine, L-Iysine, N-ethyl piperidine, dibenzylamine and the like. Those compounds (I) which are basic can form salts, including pharmaceutically acceptable salts with inorganic acids, e.g. with hydrohalic acids such as hydrochloric or hydrobromic acids, sulphuric acid, nitric acid or phosphoric acid and the like, and with organic acids e.g. with acetic, tartaric, succinic, fumaric, maleic, malic, salicylic, citric, methanesulphonic, p-toluenesulphonic, benzoic, benzenesulfonic, glutamic, lactic, and mandelic acids and the like.

For a review on suitable salts, see Handbook of Pharmaceutical Salts: Properties, Selection, and Use by Stahl and Wermuth (Wiley-VCH, Weinheim, Germany, 2002).

The term 'solvate' is used herein to describe a molecular complex comprising the compound of the invention and a stoichiometric amount of one or more pharmaceutically acceptable solvent molecules, for example, ethanol. The term 'hydrate' is employed when said solvent is water. Compounds of the invention which contain one or more actual or potential chiral centres, because of the presence of asymmetric carbon atoms, can exist as a number of enantiomers or diastereoisomers with R or S stereochemistry at each chiral centre. The invention includes all such enantiomers and diastereoisomers and mixtures thereof.

Aspects of the invention

A particular subclass of compounds for antibacterial use in accordance with the invention is concerned consists of those of formula (IA)

wherein R₄ and R₅ are independently fluoro or chloro, or one of R₄ and R₅ is hydrogen while the other is fluoro or chloro, and R₁, R₂ and R₃ are as defined with reference to formula (I) above.

Another particular subclass of compounds for antibacterial use in accordance with the invention is concerned consists of those of formula (IB)

wherein R₂ and R₃ are as defined with reference to formula (I) above.

In a narrow subclass of compounds for antibacterial use in accordance with the invention is concerned, including those of formula (IA) above, Ri and R₂ are hydrogen; and in the compounds of formula (IB) above R₂ is hydrogen. In the radical R₃, p may be 0, and m and/or n may be 1. Alternatively, p may be 1 , and Z may be an optionally substituted carbocyclic or heteroaryl radical having 3 to 6 ring atoms or an optionally substituted bicyclic carbocyclic or heteroaryl radical having 5 to 10 ring atoms, which is linked to the -(AIk¹ )_m- part of R₃ and to the - (AIk²J_n-Q part of R₃ via ring carbon or nitrogen atoms. Examples of divalent radicals Z in this embodiment include those selected from the following, in either orientation:

In another alternative embodiment p is 1, and Z is an optionally substituted monocyclic non-aromatic carbocyclic or heterocyclic radical having 3 to 6 ring atoms or an optionally substituted bicyclic non-aromatic carbocyclic or heterocyclic having 5 to 10 ring atoms, which is linked to the -(Alk¹)_m- part of R₃ and to the -(Alk²)_n-Q part of R₃ via ring carbon or nitrogen atoms. Examples of Z radicals, which are optionally substituted, in this embodiment include those selected from the following, in either orientation:

In the compounds with which the invention is concerned, and in any of the subclasses or embodiments of such compounds discussed above, Q may be hydrogen. However Q may also be a radical selected from any of the divalent Z radicals specifically identified above but with one of the unsatisfied valencies thereof satisfied with hydrogen or an optional substituent.

In the compounds with which the invention is concerned, and in any of the subclasses or embodiments of such compounds discussed above n and/or m may be 0.

In all compounds and classes of compounds with which the invention is concerned, it is typical that the radical R₃, when fully extended, does not exceed the length of an unbranched saturated hydrocarbon chain of 14 carbon atoms, ie does not exceed about 16 Angstroms. For example, that length may be equivalent to that of an unbranched saturated hydrocarbon chain of from 6 to 12, or 9 to 12 carbon atoms, ie from about 6 to about 14, and from about 10 to about 14 Angstroms respectively. In the compounds with which the invention is concerned, AIk¹ and AIk², when present, may be, for example, optionally substituted straight chain C₁-C₆ alkylene, C₂- C₆ alkenylene, or C₂-C₆ alkynylene radicals, each of which may optionally terminate with or be interrupted by -O-, -S-, -S(O)-, -S(O₂)-, -NH-, -N(CH₃)-, or -N(CH₂CH₃)-, - C(=O)-, -0-(C=O)-, -C(O)-O-.

Any optional substituents R and any optional substituents present in AIk¹, AIk², Z and Q may be selected from, for example, methyl, -OCH₃, -CF₃, -OCF₃, ethyl, cyclopropyl, oxo, hydroxyl, -F, -Cl, -Br, cyano, acetyl, amino, methylamino, dimethylamino, acetylamino, carbamate, -CONH₂, nitro, -COOH and -CH₂OH.

Compounds of formula (IC) perse, and salts, hydrates or solvates thereof constitute a distinct aspect of the invention:

wherein W is Ki(R₁)- or =N-;

R₁ is hydrogen or an optional substituent and R₂ is hydrogen, methyl, or fluoro; or R₁ and R₂ taken together are -CH₂-, -CH₂CH₂-, -0-, or, in either orientation, -O-CH₂-or -OCH₂CH₂-;

R₄ and R₅ are independently fluoro or chloro, or one of R₄ and R₅ is hydrogen while the other is fluoro or chloro;

R₃ is a radical selected from those of the following formulae A-H, in which any vacant ring position is optionally substituted:

D

wherein Q is as defined in relation to formula (I) above, and wherein any unsubstituted ring carbon is optionally substituted.

In compounds (IC) it is currently preferred that W be =CH- and R₂ be hydrogen.

In compounds (IC) Q in radical R₃ may be hydrogen or optionally substituted phenyl.

In a particular subset of compounds (IC), R₃ is optionally substituted quinolin-2-yl, benzothiazol-2-yl, thiazol-2-yl, thiazol-4-yl, thiazol-5-yl, oxadiazol-3-yl, oxadiazol-5-yl, oxazol-2-yl, oxazol-4-yl, oxazol-5-yl or thiazolopyridin-2-yl.

Optional substituents which may be present in R₃ in the compound perse aspect of the invention include methyl, -OCH₃, -CF₃, -OCF₃, ethyl, cyclopropyl, oxo, hydroxyl, - F, -Cl, -Br, cyano, acetyl, amino, methylamino, dimethylamino, acetylamino, carbamate, -CONH₂, nitro, -COOH and -CH₂OH.

Compounds of formula (ID) perse, and salts, hydrates or solvates thereof also constitute a distinct aspect of the invention:

Specific examples of compounds with which the invention is concerned include those of the Examples herein.

There are multiple synthetic strategies for the synthesis of the compounds (I) with which the present invention is concerned, but all rely on known chemistry, known to the synthetic organic chemist. Thus, compounds according to formula (I) can be synthesised according to procedures described in the standard literature and are well-known to the one skilled in the art. Typical literature sources are "Advanced Organic Chemistry", 4^th Edition (Wiley), J March, "Comprehensive Organic Transformation", 2^nd Edition (Wiley), R.C. Larock , "Handbook of Heterocyclic Chemistry", 2^nd Edition (Pergamon), A.R. Katritzky), review articles such as found in "Synthesis", "Ace. Chem. Res." , "Chem. Rev", or primary literature sources identified by standard literature searches online or from secondary sources such as "Chemical Abstracts" or "Beilstein".

Compounds (I) may be prepared, for example, by introduction of the radical -(Alk¹)_m- (Z)_p-(Alk²)_n-Q onto the hydroxyl group of a compound (II)

Further details of the synthetic approaches and schemes for the preparation of the intermediate (II) are given in the Examples herein. As mentioned above, the compounds with which the invention are concerned are antibacterially active, since they inhibit bacterial growth. They are therefore of use in the treatment of bacterial infection in humans and non-human animals e.g. other mammals, birds and fish. The compounds include those which inhibit growth of Gram-positive organisms such as Bacillus subtilis and Staphylococcus aureus and some show activity against certain Gram-negative organisms also.

It will be understood that the specific dose level for any particular patient will depend upon a variety of factors including the activity of the specific compound employed, the age, body weight, general health, sex, diet, time of administration, route of administration, rate of excretion, drug combination and the severity of the particular disease undergoing therapy. As is required in the pharmaceutical art, safe and permitted doses will be determined by clinical trial, but daily dosages can vary within wide limits and will be adjusted to the individual requirements in each particular case. Typically, however, the dosage adopted for each route of administration when a compound is administered alone to adult humans is 0.0001 to 150 mg/kg body weight. Such a dosage may be given, for example, from 1 to 5 times daily. For intravenous injection a suitable daily dose is from 0.0001 to 150 mg/kg body weight. A daily dosage can be administered as a single dosage or according to a divided dose schedule.

The compounds with which the invention is concerned may be prepared for administration by any route consistent with their pharmacokinetic properties, such as oral, topical, or sterile parenteral solutions or suspensions. The orally administrable compositions may be in the form of tablets, capsules, powders, granules, lozenges, liquid or gel preparations. Tablets and capsules for oral administration may be in unit dose presentation form, and may contain conventional excipients such as binding agents, for example syrup, acacia, gelatin, sorbitol, tragacanth, or polyvinylpyrrolidone; fillers for example lactose, sugar, maize-starch, calcium phosphate, sorbitol or glycine; tabletting lubricant, for example magnesium stearate, talc, polyethylene glycol or silica; disintegrants for example potato starch, or acceptable wetting agents such as sodium lauryl sulphate. The tablets may be coated according to methods well known in normal pharmaceutical practice. Oral liquid preparations may be in the form of, for example, aqueous or oily suspensions, solutions, emulsions, syrups or elixirs, or may be presented as a dry product for reconstitution with water or other suitable vehicle before use. Such liquid preparations may contain conventional additives such as suspending agents, for example sorbitol, syrup, methyl cellulose, glucose syrup, gelatin hydrogenated edible fats; emulsifying agents, for example lecithin, sorbitan monooleate, or acacia; non-aqueous vehicles (which may include edible oils), for example almond oil, fractionated coconut oil, oily esters such as glycerine, propylene glycol, or ethyl alcohol; preservatives, for example methyl or propyl p-hydroxybenzoate or sorbic acid, and if desired conventional flavouring or colouring agents.

For topical application to the skin, the drug may be made up into a cream, lotion or ointment. Cream or ointment formulations which may be used for the drug are conventional formulations well known in the art, for example as described in standard textbooks of pharmaceutics such as the British Pharmacopoeia.

For topical application to the eye, the drug may be made up into a solution or suspension in a suitable sterile aqueous or non aqueous vehicle. Additives, for instance buffers such as sodium metabisulphite or disodium edeate; preservatives including bactericidal and fungicidal agents such as phenyl mercuric acetate or nitrate, benzalkonium chloride or chlorhexidine, and thickening agents such as hypromellose may also be included.

The active ingredient may also be administered parenterally in a sterile medium, either subcutaneously, or intravenously, or intramuscularly, or intrasternally, or by infusion techniques, in the form of sterile injectable aqueous or oleaginous suspensions. Depending on the vehicle and concentration used, the drug can either be suspended or dissolved in the vehicle. Advantageously, adjuvants such as a local anaesthetic, preservative and buffering agents can be dissolved in the vehicle.

Since the compounds with which the invention is concerned are antibacterially active and inhibit bacterial growth, they are also of use in treating bacterial contamination of a substrate, such as hospital instruments or work surfaces. In order to treat a contaminated substrate, the compounds may be applied to the site of such contamination in an amount sufficient to inhibit bacterial growth. The following examples illustrate the synthesis of compounds with which the invention is concerned.

Analytical Method The analytical methods used to characterise compounds included HPLC-MS and ¹H NMR.

HPLC-MS Conditions - Method 1

Mobile Phase: A = Acetonitrile

B = 1OmM aqueous ammonium acetate

Gradient:

Run Time : 7 min

Flow Rate: 1 ml/min

Injection vol: variable dependant on sample concentration

Column temperature: 40 ⁰C

Column: 50 x 4.6 mm Gemini C18; 5μm

PDA Detector: 220, 240, and 254 nm analysed

HPLC-MS Conditions - Method 2

Mobile Phase: A = Acetonitrile

B = 1OmM aqueous ammonium acetate

Gradient:

Run Time : 30 min Flow Rate: 1 ml/min Injection vol: variable dependant on sample concentration Column temperature: 40 ⁰C

Column: 50 x 4.6 mm Gemini C18; 5μm

PDA Detector: 220, 240, and 254 nm analysed

HPLC-MS Conditions - Method 3

Mobile Phase: A = Acetonitrile + 0.1% Trifluoroacetic acid B = Water + 0.1% Trifluoroacetic acid Gradient:

Run time: 2.4 min

Flow rate: 1 ml/min

Injection vol: 3 μl

Column temperature: ambient (20⁰C)

Column: 50 x 2.0mm Hypersil C18 BDS; 5μm

UV Detector Variable wavelength detector set at 215nm

HPLC-MS Conditions - Method 4

Mobile Phase: A = Acetonitrile + 0.1% Formic acid B = Water + 0.1% Formic acid Gradient:

Run time: 3.5 min

Flow rate: 1 ml/min

Injection vol: 3 μl

Column temperature: ambient (2O⁰C)

Column: 50 x 2.1 mm Atlantis dC18; 5μm

UV Detector Variable wavelength detector set at 215nm HPLC Analysis Conditions - Method 5

HPLC Analysis Conditions - Method 6

HPLC Analysis Conditions - Method 7

HPLC-MS Conditions - Method 8

Run time: 30.0 min

Flow rate: 1 ml/min

Column temperature: Ambient (25°C) Column: 250 x 4.6mm Xbridge dC18; 5μm UV Detector Variable wavelength detector set at 215nm

HPLC-MS Conditions - Method 9

Mobile Phase: A = Acetonitrile + 0.1% Formic acid B = Water + 0.1 % Formic acid Gradient:

Run time: 30.0 min Flow rate: 1 ml/min

Column temperature: ambient (25°C)

Column: 250 x 4.6mm Purospher Star dC18; 5μm

UV Detector Variable wavelength detector set at 262 nm

NMR

¹H NMR spectra were consistent with the required structures.

Melting points were measured on a Stuart Scientific SMP10 apparatus and are uncorrected.

Yields given are not optimised.

Experimental Procedures

Scheme 1: (a) SOCI₂, toluene, reflux; (b) aqueous NH₃.

General Procedure for the Conversion of a Carboxylic Acid to a Carboxylic Amide (Method A). 3-Hydroxybenzenecarboxamide.

W, Y, Z = H, F, Cl P = H, CH₃

3-Hydroxybenzoic acid (110.5 g, 0.8 mol, 1 equiv.) was suspended in toluene (500 ml) and thionyl chloride (88.0 ml, 1.2 mol, 1.5 equiv.) was added slowly, at room temperature. The solution was heated to reflux where it was maintained for 5 h. After this time, the reaction was cooled to room temperature and concentrated in vacuo. The residue was dissolved in tetrahydrofuran (300 ml) and cooled in an ice-methanol bath. Concentrated aqueous ammonia solution (~300 ml) was added slowly, dropwise and the reaction mixture was warmed slowly to room temperature where it was stirred for 16 h. The reaction mixture was concentrated in vacuo and the resulting solid was suspended in water and filtered. The collected solid was washed with additional water (x3) and then dried in vacuo to give 3-hydroxybenzamide as an off-white solid (79.9 g, 72.8%) mp 167-168⁰C. HPLC-MS (method 1): m/z 136 [M-H]\ Rt = 1.21 min. ¹H NMR (d₆-DMSO) δ = 9.53 (s, 1H), 7.78 (s, 1H), 7.30-7.15 (m, 4H), 6.88 (d, J = 8 Hz, 1 H).

Scheme 2: (a) RX, K₂CO₃, NaI, DMF, 60⁰C.

General Procedure for the Alkylation of Phenols Using Alkyl Halides (Method

B).

W, Y, Z = H₁ F, Cl X = Br, Cl

Example 1: 3- Nonyloxy-benzenecarboxamide.

To a solution of 3-hydroxybenzenecarboxamide (200 mg, 1.46 mmol, 1 equiv.) in DMF (3 ml) was added K₂CO₃ (302 mg, 2.19 mmol, 1.5 equiv.) and NaI (43.5 mg, 0.29 mmol, 0.2 equiv). The suspension was stirred for 5 min before n-nonyl chloride (0.32 ml, 1.61 mmol, 1.1 equiv) was introduced. The resulting mixture was warmed to 60⁰C where it was maintained for 16 h. After this time, the reaction was cooled to room temperature and partitioned between EtOAc and water. The organic phase was separated, washed with additional water (x 2), dried (MgSO₄), filtered and concentrated in vacuo to reveal a colourless solid. In the case of 3-n- nonyloxybenzamide this colourless solid was stirred for 5 min with MeOH (~0.5 ml) [NB: 3-n-nonyloxybenzamide partially soluble in MeOH] and then filtered to reveal the desired compound as a colourless solid (116 mg, 30%). HPLC-MS (method 3): m/z 264 [M+H]⁺, Rt = 1.80 min. ¹H NMR (d₆-DMSO) δ = 7.95 (s, 1 H), 7.44-7.31 (m, 4H), 7.06 (ddd, J = 8 Hz, J = 2 Hz, J = 1 Hz, 1 H), 3.99 (t, J = 6.5 Hz, 2H), 1.72 (quintet, J = 6.5 Hz, 2H), 1.42 (m, 2H), 1.34-1.26 (m, 10H), 0.86 (t, J = 6.5 Hz, 3H).

NB 1 : The final purification step was dependent on the nature of the R group. Other purification methods used in course of the library synthesis were:

1. Recrystallisation (e.g. neat MeOH, EtOAc/hexanes, CH₃CN).

2. Normal phase column chromatography (silica gel). 3. Preparative HPLC or preparative TLC.

NB 2: In the case of water soluble target compounds, the aqueous phase was concentrated in vacuo and then washed with MeOH. The methanolic fractions were concentrated in vacuo and the crude product purified by preparative HPLC.

Examples 2 to 44 (Table A)

Examples 2 to 44 were synthesised according to Method B, scheme 2

I 6 I 7 I 8 I 9 I

23 24 25

Table of names of product compounds; Examples 2-44:

Scheme 3: (a) ROH, PPh₃-PS, DIAD, Et₃N, THF, r.t.

General Procedure for the AIkylation of Phenols Using Alcohols via Mitsunobu reaction (Method C).

W, Y, Z = H, F, Cl

Example 45 3-r(Z)-5-Decenyloxv1benzenecarboxamide

To a suspension of polymer-supported triphenyl phosphine (1.4 g, 3 mmol, based on a loading of 2.15 mmol/g [purchased from Argonaut], 1.5 equiv.) swollen in THF (20 ml) at room temperature was added diisopropylazodicarboxylate (0.47 ml, 2.4 mmol, 1.2 equiv.). The mixture was shaken for 5 min before 3-hydroxybenzamide (274 mg, 2 mmol, 1 equiv.), triethylamine (0.28 ml, 2 mmol, 1 equiv.) and cis-5-decenol (313 mg, 2 mmol, 1 equiv.) were added. The resulting suspension was shaken at room temperature for 16 h and then filtered. The resin was washed with additional THF (x 3) and then the combined filtrate and washings were concentrated under reduced pressure, to give the crude product as a colourless semi-solid. It was purified by column chromatography on silica eluting with EtOAc/hexane (20%-40% gradient) to give the desired compound as a white solid (390 mg, 71%), mp 98- 100⁰C. HPLC-MS (method 1): m/z 276 [M+H]⁺, Rt = 5.00 min. ¹H NMR (CDCI₃) δ = 7.35 (s, 1 H), 7.32-7.28 (m, 2H), 7.08-7.02 (m, 1H), 6.18 (br, 2H), 5.41-5.32 (m, 2H), 3.98 (t, J = 6.4 Hz, 2H), 2.12-2.05 (m, 2H), 2.05-1.98 (m, 2H), 1.79 (m, 2H), 1.51 (m, 2H), 1.34-1.28 (m, 4H), 0.88 (t, J = 7.0 Hz, 3H).

NB 1 : In some cases diethylazodicarboxylate (0.38 ml, 2.4 mmol, 1.2 equiv.) was used instead of diisopropylazodicarboxylate.

NB 2: In some cases unsupported triphenyl phosphine was used. In the case of phenols containing fluorine atoms, no product could be detected when using polymer-supported triphenyl phosphine and so the reactions were performed with triphenyl phosphine.

Examples 46-61 (Table B)

Examples 46 to 61 were synthesised according to Method C, scheme 3

Table of names of product compounds; Examples 46-61:

Scheme 4: (a) Br(CH₂)₆Br, K₂CO₃, CH₃CN, 60⁰C; (b) PPh₃, CH₃CN, reflux; (c) (i) KHMDS, toluene, 0⁰C; (ii) RCHO, -78°C to r.t.; (d) H₂, 10% Pd/C, MeOH, r.t.

~ 9:1 (Z : £)

~ 8:2 (Z : E)

3-[(6-Bromohexyl)oxy]benzenecarboxamide.

(Method D) K₂CO₃ (1.38 g, 10 mmol, 1 equiv.) was added to a suspension of 3- hydroxybenzamide (1.37 g, 10 mmol, 1 equiv.) in CH₃CN (100 ml). The mixture stirred for 10 min at room temperature, before 1 ,6-dibromo-hexane (9.76 g, 40 mmol, 4 equiv.) was added. The resulting mixture was stirred at 60⁰C for 16 h. After this time, the reaction was cooled to room temperature, any undissolved solids were filtered off and the filtrate evaporated under reduced pressure to dryness. The residue was taken-up in EtOAc and water. The organic phase was separated and washed consecutively with K₂CO₃ solution, water and brine. Dried with MgSO₄ and evaporated under reduced pressure to a small volume. The precipitant solid was filtered and washed with EtOAc/pentane, to give the desired compound as a white solid (2.0 g, 67%), mp 115-117⁰C. HPLC-MS (method 1): m/z 300 [M]⁺, 302 [M+2H]⁺, Rt = 4.08 min.

6-[3-(Aminocarbonyl)phenoxy]hexyl(triphenyl)phosphonium bromide.

-

Br

A mixture of 3-[(6-bromohexyl)oxy]benzenecarboxamide (2.10 g, 7 mmol, 1 equiv.) and triphenylphosphine (1.93 g, 7.35 mmol, 1.05 equiv.) in CH₃CN (30 ml) was heated under reflux for 72 h. The solvent was evaporated under reduced pressure and the residue was triturated with dry Et₂O until it solidified. The solid was filtered and dried in vacuo to give the desired compound as a white solid (4.0 g, 100%). HPLC-MS (method 1): m/z 482 [M-Br]⁺, Rt = 3.65 min.

Example 62: 3-fr(Z)-7-(3-Thienyl)-6-heptenvπoxv)benzenecarboxamide.

(Method E) To a stirred suspension of 6-[3-(aminocarbonyl)phenoxy]hexyl (triphenyl) phosphonium bromide (2.0 g, 3.55 mmol, 1.2 equiv.) in anhydrous toluene (28 ml) was added a solution of potassium bis(trimethylsilyl)amide (0.5M; 7.1 ml, 3.55 mmol, 1.2 equiv.) in toluene, slowly, dropwise over a period of 15 min at 0⁰C, under N₂. The dark orange solution was stirred for another 20 min at 0⁰C and cool-d to -78°C, when thiophene-3-carboxaldehyde was instantly added, and the temperature was left to rise-from -78°C to r.t. The light yellow mixture was stirred at r.t. for 16h. The reaction mixture was quenched with saturated aqueous NH₄CI (20ml) and the solvent was evaporated under reduced pressure. The residue was taken-up in CH₂CI₂ and H₂O, the organic phase was separated, washed with brine and dried (Na₂SO₄). The solved was evaporated under reduced pressure and the residue was purified by column chromatography on silica eluting with EtOAc/hexane (10%-50% gradient) to give the desired compound as an off-white solid (300 mg, 35%), mp 71- 73°C. By ¹H NMR analysis it consisted of a mixture of ZE (90:10). HPLC-MS (method 1): m/z 316 [M+H]⁺, Rt = 4.62 min.

Example 63: 3-fr7-(3-Thienvl)heptyl1oxv)benzenecarboxamide.

To a solution of example 62 3-{[(Z)-7-(3-Thienyl)-6-heptenyl]oxy}benzene carboxamide (260 mg, 0.82 mmol) in MeOH (8 ml), 10% Pd/C (30 mg) was added. The mixture was stirred under H₂ at r.t for 3 days. The catalyst was removed by filtration through a pad of Celite and the solvent was evaporated under reduced pressure, to a small volume. The precipitant solid was filtered and rinsed with Et₂O/pentane to give the desired compound as a white solid (130 mg, 48%), mp 97- 100⁰C. HPLC-MS (method 1): m/z 318 [M+H]⁺, Rt = 4.87 min.

Example 64: 3-(r(Z)-7-(5-Chloro-2-fun/l)-6-heptenvnoxv)benzenecarboxamide.

Synthesised from 6-[3-(aminocarbonyl)phenoxy]hexyl(triphenyl) phosphonium bromide according to Method E. Yield 72%, mp 53-56°C. By ¹H NMR analysis it consisted of a mixture of ZE (81 :19). HPLC-MS (method 1): m/z 334 [M+H]⁺, Rt = 4.80 min.

Scheme 5: (a) Br(CH₂)_nBr (n=5, 8) K₂CO₃, CH₃CN, 60⁰C; (b) Lithium acetylide ethylenediamine complex [LiCsCH(H₂NCH₂CH₂NH₂)], DMSO, r.t.

3-[(7-Bromoheptyl)oxy]benzenecarboxamide.

Synthesised according to Method D. HPLC-MS (method 1): m/z 314 [M]⁺, 316 [M+2H]⁺, Rt = 4.37 min.

Example 65: 3-(8-Nonvnvloxv)benzenecarboxamide.

(Method F) Lithium acetylide ethylenediamine complex (305 mg, 3.3 mmol, 1.1 equiv.) was placed in a three-neck flask, degassed, flushed with N₂ and suspended in DMSO (2 ml). To the stirred suspension a solution of 3-[(7- bromoheptyl)oxy]benzenecarboxamide (943 mg, 3 mmol, 1 equiv.) in DMSO (2 ml), was added, slowly, dropwise, at r.t., under N₂. The reaction mixture was stirred at r.t. for 16 h. After that time it was diluted with 1 N HCI solution and extracted with EtOAc (x 3). The combined organic extracts were washed with brine, dried (Na₂SO₄) and evaporated to dryness under reduced pressure. The crude product was purified by column chromatography on silica eluting with EtOAc/hexane 20%, to give the desired compound as a white solid (100 mg, 13%), mp 82-83°C. HPLC-MS (method 1): m/z 260 [M+H]⁺, Rt = 4.26 min.

3-[(7-Bromodecyl)oxy]benzenecarboxamide.

Synthesised according to Method D. Yield 32%, mp 114-116°C, HPLC-MS (method 1): m/z 356 [M]⁺, 358 [M+2H]⁺, Rt = 5.15 min.

Example 66: 3-(11-Dodecynyloxy)benzenecarboxamide.

Synthesised from 3-[(7-bromodecyI)oxy]benzenecarboxamide according to Method F; mp 106-108⁰C, HPLC-MS (method 1): m/z 302 [M+H]⁺, Rt = 5.02 min.

Scheme 6: (a) Lithium acetylide ethylenediamine complex [LiCsCH(H₂NCH₂CH₂NH₂)], DMSO, r.t.; (b) p-toluenesulfonic acid, EtOH, reflux; (c) 3-hydroxybenzenecarboxamide, PPh₃-PS, DIAD, Et₃N, THF, r.t.

(b) (C) 10-Undecyn-i-ol.

HO'

^CH

(Method G) A solution of commercially available 2-[(8-bromooctyl)oxy]tetrahydro-

2H-pyran (1.0 g, 3.4 mmol, 1 equiv.) in DMSO (5 ml), was added, slowly, dropwise, at r.t., under N₂, to a stirred suspension of lithium acetylide ethylenediamine complex (350 mg, 3.8 mmol, 1.1 equiv.) in DMSO (5 ml). The reaction mixture was stirred at r.t. for 18 h and diluted with n-pentane (50 ml). The organic phase was washed with 1N HCI solution (2x20 ml) and water (2x20 ml), dried (Na₂SO₄) and evaporated to dryness under reduced pressure. The residue (colourless liquid, 570 mg, yield 70%) was dissolved in 95% EtOH (20 ml) together with p-toluenesulfonic acid (150 mg) and the mixture was heated under reflux for 2.5 h. After being cooled, the solvent was evaporated under reduced pressure. The residue was purified by column chromatography on silica eluting with EtOAc/hexane (10%-30% gradient), to give the desired compound as a colourless oil (240 mg, overall yield 48%).

Example 67: 3-(9-Decynyloxv)benzenecarboxamide.

Synthesised from 3-hydroxybenzenecarboxamide and 10-undecyn-1-ol according to Method C, scheme 3; mp 111-112°C, HPLC-MS (method 1): m/z 274 [M+H]⁺, Rt = 4.61 min.

Scheme 7: (a) SOCI₂, toluene, reflux; (b) aqueous NH₃; (c) n-Non-Br, K₂CO₃, NaI, DMF, 60⁰C; (d) 10-undecynol, PPh₃-PS, DIAD, Et₃N, THF, r.t.

2-Chloro-5-hydroxybenzenecarboxamide.

Synthesised from commercially available 2-chloro-5-hydroxybenzenecarboxylic acid, according to Method A, scheme 1. Yield 28%, mp 159-161⁰C, HPLC-MS (method 1): m/z 170 [M-H]^", Rt = 1.48 min.

Example 68: 2-Chloro-5-(nonyloxv)benzenecarboxamide.

Synthesised from 2-chloro-5-hydroxybenzenecarboxamide according to Method B, scheme 2. Yield 80%, HPLC-MS (method 1): m/z 339 [M+H+CH₃CN]⁺, Rt = 5.29 min.

Example 69: 2-Chloro-5-(10-uπdecynyloxy)benzenecarboxamide.

Synthesised from 2-chloro-5-hydroxybenzenecarboxamide according to Method C, scheme 3. Yield 13%, HPLC-MS (method 1): m/z 322 [M+H]\ Rt = 4.94 min.

Scheme 8: (a) BBr₃, CH₂CI₂, r.t.; (b) R₁-Br, K₂CO₃, NaI, DMF, 60°C; (c) R₂-OH, PPh₃- PS, DIAD, Et₃N, THF, r.t.

R₁ = nonyl, decyl, undecyl

R₂ = 2-nonenyl, 10=undecynyl

2-Fluoro-5-hydroxybenzenecarboxamide.

(Method H) Boron tribromide solution (1.0 M in CH₂CI₂, 23.6 ml, 23.6 mmol, 2 equiv.) was added slowly, dropwise to stirred solution of 2-fluoro-5- methoxybenzenecarboxamide (2.0 g, 11.8 mmol, 1 equiv.) in CH₂CI₂ (60 ml), at r.t., under N₂. The reaction mixture was stirred at r.t. for 48 h. The solvent was removed under reduced pressure, the residue was dissolved in water (120 ml) and extracted with EtOAc (4x100 ml). The combined organic extracts were washed with water (2x100 ml), dried (Na₂SO₄) and filtered through a pad of silica gel. The filtrate was evaporated to dryness under reduced pressure, to give the desired compound as a grey solid (1.5O g, 82%).

Examples 70-75 (Table C)

Examples 70-72 were synthesised from 2-fluoro-5-hydroxybenzenecarboxamide according to Method B, scheme 2 and Examples 73-75 were synthesised from 2- fluoro-5-hydroxybenzenecarboxamide according to Method C, scheme 3.

Table of names of product compounds; Examples 70-75:

Scheme 9: (a) SOCI₂, toluene, reflux; (b) aqueous NH₃; (c) BBr₃, CH₂CI₂, r.t.; (d) n- Non-Br, K₂CO₃, NaI, DMF, 60⁰C.

β-Chloro^-fluoro-S-methoxybenzenecarboxamide.

Synthesised from commercially available 6-chloro-2-fluoro-3- methoxybenzenecarboxylic acid according to Method A, scheme 1. Yield 85%, mp 154-156°C, HPLC-MS (method 1): m/z 245 [M+H+CH₃CN]⁺, Rt = 2.37 min.

β-Chloro^-fluoro-S-hydroxybenzenecarboxamide.

Synthesised from δ-chloro^-fluoro-S-methoxybenzenecarboxamide according to Method H. Yield 90%.

Example 76: 6-Chloro-2-fluoro-3-(nonvloxy)benzenecarboxamide.

Synthesised from θ-chloro^-fluoro-S-hydroxybenzenecarboxamide according to Method B, scheme 2. Yield 73%, mp 75-77°C, HPLC-MS (method 1): m/z 316 [M+H]⁺, Rt = 5.27 min.

Scheme 10: (a) BBr₃, CH₂CI₂, r.t; (b) R-Br, K₂CO₃, NaI, DMF, 60⁰C.

hexyl, nonyl

2-Chloro-6-fluoro-3-hydroxybenzenecarboxamide.

Synthesised from commercially available 2-chloro-6-fluoro-3- methoxybenzenecarboxamide, according to Method H. Yield 78%.

Example 77: 2-Chloro-6-fluoro-3-(hexvloxv)benzenecarboxamide.

Synthesised from 2-chloro-6-fluoro-3-hydroxybenzenecarboxamide according to Method B, scheme 2. Yield 30%, mp 66-68⁰C, HPLC-MS (method 1): m/z 27 '4 [M+H]⁺, Rt = 2.78 min.

Example 78: 2-Chloro-6-fluoro-3-(nonyloxy)benzenecarboxamide.

Synthesised from 2-chloro-6-fluoro-3-hydroxybenzenecarboxamide according to Method B, scheme 2. Yield 15%, mp 64-66°C, HPLC-MS (method 1): m/z 316 [M+H]⁺, Rt = 5.13 min.

Scheme 11: (a) SOCI₂, toluene, reflux; (b) aqueous NH₃; (c) BBr₃, CH₂CI₂, r.t; (d) n- Hex-Br, K₂CO₃, NaI, DMF, 6O⁰C.

2,4,6-Trifluoro-3-methoxybenzenecarboxamide.

Synthesised from commercially available 2,4,6-trifluoro-3- methoxybenzenecarboxylic acid, according to Method A, scheme 1. Yield 85%, mp 102⁰C, HPLC-MS (method 1): m/z 206 [M+H]⁺, Rt = 2.40 min.

2,4,6-Trifluoro-3-hydroxybenzenecarboxamide.

Synthesised from 2,4,6-trifluoro-3-methoxybenzenecarboxamide according to Method H. Yield 100%, HPLC-MS (method 1): m/z 190 [M-H]^", Rt = 1.07 min.

Example 79: 2.4.6-Trifluoro-3-(hexvloxv)benzenecarboxamide.

Synthesised from 2,4,6-trifluoro-3-hydroxybenzenecarboxamide according to Method B, scheme 2. Yield 54%, mp 89-90⁰C, HPLC-MS: m/z 276 [M+H]⁺, Rt = 4.36 min.

Scheme 12: (a) BBr₃, CH₂CI₂, r.t.; (b) n-Hex-Br, K₂CO₃, NaI, DMF, 60⁰C.

2,4-Difluoro-3-hydroxybenzenecarboxamide.

Synthesised from commercially available 2,4-difluoro-3- methoxybenzenecarboxamide according to Method H. Yield 98%, HPLC-MS (method 1): m/z 172 [M-H]^", Rt = 1.03 min.

Example 80: 2,4-Difluoro-3-(hexyloxy)benzenecarboxamide.

Synthesised from 2,4-difluoro-3-hydroxybenzenecarboxamide according to Method B, scheme 2. Yield 51%, mp 86-87°C.

Scheme 13: (a) SOCI₂, toluene, reflux; (b) aqueous NH₃; (c) BBr₃, CH₂CI₂, r.t; (d) R₁- Br, K₂CO₃, NaI, DMF, 6O⁰C; (e) R₂-OH, PPh₃-PS, DIAD, Et₃N, THF, r.t.

2,6-Difluoro-3-methoxybenzenecarboxamide.

Synthesised from commercially available 2,6-difluoro-3- methoxybenzenecarboxylic acid according to Method A, scheme 1. Yield 84%, mp 167-169°C, HPLC-MS (method 1): m/z 188 [M+H]\ Rt = 2.00 min.

2,6-Dif luoro-3-hydroxy benzenecarboxamide.

Synthesised from 2,6-difluoro-3-methoxybenzenecarboxamide according to Method H. Yield 78%. HPLC-MS (method 1): m/z 172 [M-H]^", Rt = 1.25 min

Examples 81-87 (Table D)

Examples 81-83 were synthesised from 2,6-difluoro-3-hydroxybenzenecarboxamide according to Method B, scheme 2. Examples 84-88 were synthesised from 2,6- difluoro-3-hydroxybenzenecarboxamide according to Method C, scheme 3.

Table of names of product compounds; Examples 81-88:

Scheme 14: (a) K₂CO₃, DMF, r.t.; (b) NaOH, H₂O/IPA, reflux; (c) n-Hex-Br, K₂CO₃, DMF, 70⁰C.

Methyl 2-[3-(aminocarbonyl)-2,4-difluorophenoxy]acetate.

A mixture of 2,6-difluoro-3-hydroxybenzenecarboxamide (1.2 g, 7 mmol, 1 equiv.), K₂CO₃ (2.87 g, 21 mmol, 3 equiv.) and methyl bromoacetate (.69 ml, 7.35 mmol, 1.05 equiv.) in DMF (30 ml) was stirred at r.t. for 18 h. The mixture was diluted with water and extracted with EtOAc (4x80 ml). The combined organic extracts were dried (MgSO₄) and evaporated to dryness under reduced pressure. The product was used crude on the next step. HPLC-MS (method 1): m/z 246 [M+H]⁺, Rt = 2.08 min.

2-[3-(Aminocarbonyl)-2,4-difluorophenoxy]acetic acid.

Methyl 2-[3-(aminocarbonyl)-2,4-difluorophenoxy]acetate (7 mmol, 1 equiv.) was added to a solution of NaOH (1 g, 25 mmol, 3.6 equiv.) in water (20 ml) and isopropyl alcohol (5 ml). The mixture was stirred under reflux for 1.5 h, diluted with water (40 ml) and extracted with CH₂CI₂ (40 ml). The aqueous phase was acidified to pH 1 with cone. HCI solution. The precipitant solid was filtered and dried in vacuo to give the desired compound (130 mg, 8%), mp 152-153°C. HPLC-MS (method 1): m/z 312 [M- H+2CH₃CN]-, Rt = 0.91 min.

Example 89: Hexyl 2-[3-(aminocarbonyl)-2,4-difluorophenoxy]acetate.

n-Bromohexane (0.077 ml, 0.55 mmol, 1.05 equiv.) was added to a suspension of 2- [3-(aminocarbonyl)-2,4-difluorophenoxy]acetic acid (120 mg, 0.52 mmol, 1 equiv.) and K₂CO₃ (215 mg, 1.56 mmol, 3 equiv.) in DMF (3 ml) and the mixture was stirred at 70⁰C for 1.5 h. After cooling at r.t., the mixture was poured into water (25 ml) and the precipitant solid was filtered and washed with water (2x20 ml). After drying, the crude solid was triturated by stirring in hexane (10 ml), filtered and washed with hexane (3x10 ml), to give the desired compound as a white solid (99 mg, 60%), mp 108⁰C. HPLC-MS: m/z 316 [M+Hf, Rt = 4.09 min.

Scheme 15: (a) Lithium acetylide ethylenediamine complex

[LiC=CH(H₂NCH₂CH₂NH₂)], DMSO, r.t.; (b) p-toluenesulfonic acid, EtOH, reflux; (c) CICH₂COCI, CH₂CI₂, r.t; (d) K₂CO₃, NaI, DMF, 60⁰C.

7-Octyn-1-ol.

^CH

Synthesised from commercially available 2-[(8-bromohexyl)oxy]tetrahydro-2/Y- pyran according to Method G. Overall yield 55%, colourless oil.

7-Octynyl 2-chloroacetate.

Chloroacetyl chloride (0.16 ml, 2.0 mmol, 1 equiv.) was added to a stirred solution of 7-Octyn-1-ol (300 mg, 2.4 mmol, 1.2 equiv.) in CH₂CI₂ (6 m-) at -5°C. The reaction mixture was allowed to warm-up to r.t., were it was stirred for 4 h. The solvent was removed under reduced pressure and the residue was purified by column chromatography on silica eluting with EtOAc/hexane (10%) to give the desired compound as a pale yellow liquid (450 mg, 100%).

Example 90: 7-Octynyl 2-[3-(aminocarbonyl)-2,4-difluorophenoxy]acetate.

Synthesised from 2,6-difluoro-3-hydroxybenzenecarboxamide according to Method B, scheme 2. Yield 13%, mp 130-132⁰C, HPLC-MS (method 1): m/z 340 [M+H]⁺, Rt = 3.93 min.

Scheme 16: (a) ZnEt₂, CH₂I₂, toluene, r.t.

Example 91 : 3-r4-(2-Ethvlcvclopropyl)butoxv1ben2enecarboxamide.

A solution of diethyl zinc (1.1 M in toluene, 1.84 ml, 2.02 mmol, 1 equiv.) was added to a solution of example 52 (500 mg, 2.02 mmol, 1 equiv.) in dry toluene (1 ml), at r.t., under N₂. Diiodomethane (0.244 ml, 3.03 mmol, 1.5 equiv.) was added slowly, dropwise and the reaction mixture was stirred at r.t. for 5 days. The mixture was diluted with water (40 ml) and extracted with CH₂CI₂ (4x40 ml). The combined organic extracts were dried (MgSO₄) and the solvents were removed under reduced pressure. By HPLC-MS, the crude residue consisted of starting material (80%) and desired product (20%). The reaction was repeated in the same way, in tolune (15 ml) using diethyl zinc (1.1 M in toluene, 6.1 ml, 6.6 mmol, 3.3 equiv.) and diiodomethane (0.244 ml, 3.03 mmol, 1.5 equiv.). The reaction mixture was stirred at 50⁰C for 5 days, diluted with water (80 ml) and extracted with CH₂CI₂ (4x50 ml). The combined organic extracts were dried (MgSO₄) and the solvents were removed under reduced pressure. The residue was triturated by stirring in pentane (15 ml) and the precipitant solid was filtered and rinsed with pentane to give 196 mg of a white compound, mp 104-105⁰C. By HPLC-MS it consisted of starting material (65%) and the desired product (35%). HPLC-MS (method 1): m/z 303 [M+H+CH₃CN]⁺, Rt = 4.83 min.

Scheme 17: (a) Br(CH₂)₉OH, K₂CO₃, NaI, DMF, 60⁰C; (b) toluenesulfonyl chloride, Et₃N, CH₂CI₂, r.t.; (c) NaCN, H₂O/EtOH, 75°C.

3-[(9-Hydroxynonyl)oxy]benzenecarboxamide.

Synthesised according to Method B, scheme 2. Yield 75%, mp 118-120⁰C, HPLC-MS (method 1): m/z 280 [M+H]⁺, Rt = 3.50 min.

Example 92: 9-[3-(Aminocarbonyl)phenoxy]nonyl 4-methylbenzenesulfonate.

Toluenesulfonyl chloride (410 mg, 2.15 mmol, 1.5 equiv.) and triethylamine (0.40 ml, 2.88 mmol, 2 equiv.) were added to a solution of 3-[(9- hydroxynonyl)oxy]benzenecarboxamide (400 mg, 1.43 mmol, 1 equiv.) in CH₂CI₂ (4 ml) and the reaction mixture was stirred at r.t. for 6 days. Saturated NaHCO₃ solution (40 ml) was added and the mixture was extracted with CH₂CI₂ (3x30 ml). The combined organic extracts were dried (MgSO₄) and the solvent was evaporated under reduced pressure. The residue was purified by column chromatography on silica eluting with CH₃OH/CH₂CI₂ (2%), to give the desired compound as white solid (428 mg, 69%), mp 78-80⁰C. HPLC-MS (method 1): m/z 434 [M+H]⁺, Rt = 4.90 min.

Example 93: 3-r(9-Cvanononvl)oxvlbenzenecarboxamide.

Sodium cyanide (60 mg, 1.22 mmol, 1.3 equiv.) was added to a solution of 9-[3- (aminocarbonyl)phenoxy]nonyl 4-methylbenzenesulfonate (407 mg, 0.94 mmol, 1 equiv.) in water (10 ml) and 95% EtOH (8 ml), and the reaction mixture was stirred at 75°C for 2 days. After cooling at r.t., the mixture was diluted with water (10 ml) and extracted with CH₂CI₂ (3x10 ml). The combined organic extracts were dried (MgSO₄) and the solvent was removed under reduced pressure. The crude residue was purified by column chromatography on silica eluting with EtOAc/hexane (50%), to give the desired compound as white solid (57 mg, 21%), mp 96-97°C. HPLC-MS (method 1): m/z 289 [M+H]\ Rt = 4.16 min.

Scheme 18: (a) n-Non-Br, K₂CO₃, NaI, DMF, 60⁰C; (b) LiOH, NaOCH₃, MeOH, reflux.

2-(Nonyloxy)isonicotinonitrile.

Synthesised from commercially available 2-hydroxyisonicotinonitrile according to Method B. Yield 30%, semi-solid, HPLC-MS (method 2): m/z 288 [M+H+CH₃CN]\ Rt = 21.46 min. The reaction gave also as by-product 1-nonyl-2-oxo-1,2-dihydro-4- pyridinecarbonitrile, yield 39%, mp 46-48°C, HPLC-MS (method 1): m/z 288 [M+H+CH₃CN]⁺, Rt = 4.94 min.

Example 94: 2-(Nonyloxy)isonicotinamide.

A solution of 2-(nonyloxy)isonicotinonitrile (250 mg, 1.0 mmol, 1 equiv.) and sodium methoxide (10 mg, 0.1 mmol, 0.1 equiv.) in dry CH₃OH (10 ml) was stirred at r.t. for 2.5 h. A solution of lithium hydroxide (24 mg, 1.0 mmol, 1 equiv.) in water (1 ml) was added and the reaction mixture was heated under reflux for 3.5 h. After cooling at r.t., the mixture was poured into water (40 ml). The precipitant solid was filtered and dried in vacuo at 50⁰C, to give the desired compound as a white solid (60 mg, 23%), mp 108-110⁰C. HPLC-MS (method 1): m/z 265 [M+H]⁺, Rt = 5.08 min.

Scheme 19: (a) Br₂, CCI₄, (b) K₂CO₃, CH₃CN, 60⁰C, 5 days, (c) cone. H₂SO₄, H₂O, 40⁰C.

1 ,2-Dibromoheptane

Bromine (1.9 ml, 37.28 mmol, 1.05 equiv.) was added slowly, dropwise, to a solution of 1-heptene (5 ml, 35.5 mmol, 1 equiv.) in CCI₄ (7 ml) cool-d at -10°C, under N₂. The reaction mixture was stirred at r.t. for 16 h. The solvent was removed by evaporation under reduced pressure. The residue was partitioned between CH₂CI₂ (200 ml) and 10% aqueous sodium metabisulfate solution (200 ml). The organic phase was separated, washed with brine and dried (Na₂SO₄). It was evaporated under reduced pressure to dryness, to give the desired compound as a colourless oil (8.94 g, 98%).

3-Pentyl-2,3-dihydro-1 ,4-benzodioxine-6-carbonitrile and 2-pentyl-2,3-dihydro- 1,4-benzodioxine-6-carbonitrile.

1,2-Dibromoheptane (5.11 g, 19.8 mmol, 1.1 equiv.) was added to a mixture of di- hydroxy benzonitrile (2.43 g, 18 mmol, 1 equiv.) and K₂CO₃ (12.4 g, 90 mmol, 5 equiv.) in CH₃CN (100 ml). The reaction mixture was heated under reflux for 4 days. After cooling to r.t., the solvent was removed under reduced pressure; the residue was diluted with water (200 ml) and extracted with EtOAc (3x150 ml). The combined organic phases were washed with brine, dried (Na₂SO₄) and evaporated under reduced pressure to dryness. The residue was purified by column chromatography on silica eluting with EtOAc/hexane (5%-10% gradient) to give the desired compound as a colourless oil (390 mg, 9%); mixture of two regio-isomers. HPLC-MS (method 1): m/z 230 [M-H]^", Rt = 5.28 min.

Example 95: 3-Pentyl-2,3-dihydro-1,4-benzodioxine-6-carboxamide and 2- pentyl-2,3-dihydro-1,4-benzodioxine-6-carboxamide.

A mixture of regio-isomers 3-pentyl-2,3-dihydro-1,4-benzodioxine-6-carbonitrile and 2~pentyl-2,3-dihydro-1,4-benzodioxine-6-carbonitrile (50 mg, 0.22 mmol) was stirred vigorously in cone. H₂SO₄ (0.5 ml) and warmed to 40⁰C. Water (82 mg) was added dropwise and the mixture was stirred for 45 min at 40°C. The mixture was cooled at -5°C, and ice (25 ml) was added quickly, with vigorous stirring. The mixture stirred at r.t. for two more hours. The precipitant solid was filtered, washed with water and dried in vacuo, at 40⁰C. It was purified on preparative TLC plate (Analtech, 2mm, 20x20) eluting with methyl-te/f-butyl-ether, to give the desired compound as a white solid (50 mg, 93%), HPLC-MS (method 1): m/z 291 [M+H+CH₃CN]⁺, Rt = 4.14 min.

Examples 96-99, 101-116, 117. 119, 122. 124, 128-134. 137-139, 142, 144-154. 156-159 and 161-163 (Table E)

The compounds of Examples 96-99, 101-116, 117, 119, 122, 124, 128-134, 137-139, 142, 144-154, 156-159 and 161-163 were synthesized according to the following general procedure: To a solution of reactant (A) in anhydrous DMF (B), 2,6-difluoro- 3-hydroxybenzamide (C) and potassium carbonate (D) were added. The reaction mixture was stirred at room temperature or 25 ⁰C under nitrogen atmosphere. The reaction mixture was evaporated to dryness under reduced pressure and the residue was purified by column chromatography on silica (230-400μ) using ethyl acetate/hexane as the eluent to provide the product compound.

98 99

2,6-Difluoro-3-(7-methoxy-quinolin-2-ylmethoxy)- 3-[4-(2-Chloro-phenyl)-thiazol-2-ylmethoxy]- benzamide 2,6-difluoro-benzamide

2-Bromomethyl-7-methoxy-quinoline 2-Bromomethyl-4-(2-chloro-phenyl)-thiazole

0.01 g, .00039 mol; 2 ml; 0.068 g, .00039 mol; 0.35 g, .0012 mol; 15 ml; 0.21 g, .0012 mol; 0.188 g, .0013 mol 0.585 g, .0042 mol

25⁰C / 24 h 25°C / 24 h

20:80 20:80

0.012 g, 9%, off white solid 0.80 g, 17%, yellow brown solid

3.92 (S, 3H), 5.41 (s, 2H), 7.06 (m, 1 H, J=9.2 Hz

(o-coupling) J=1.2 Hz (m-coupling), 7.25-7.32 (m, 5.59 (s, 2H), 7.13 (dt, 1 H, J=8.8 Hz (o-

2H), 7.39 (1H, J=2.0 Hz (m-coupling), 7.51 (d, coupling), J= 2.0 Hz (m-coupling), 7.39-7.48

1H, J=8.4 Hz (o-coupling), 7.88 (d, 1 H, J=4.8 (m, 3H), 7.57-7.59 (m, 1H), 7.85-7.86 (m, 1 H),

Hz), 7.91 (broad s, 1H), 8.16 (s, 1 H), 8.33 (d, 1H, 7.89 (broad s, 1H), 8.17 (broad s, 2H) J=8.4 Hz (o-coupling) 345.06 381.03 5, 8.73 5, 9.99

122 124

2,6-Difluoro-3-(4-phenethyl-thiazol-2-ylmethoxy)- 3-[1-(5-Chloro-benzothiazol-2-yl)-ethoxy]-2,6- benzamide difluoro-benzamide

2-bromomethyl-4-phenethyl-thiazole 2-(1-bromo-ethyl)-5-chloro-benzothiazole

0.20Og, 0.7mmol; 5 ml; 0.125g, OJmmol; 0.30Og, 0.3 g, 0.1 mmol; 2 ml; 0.188 g, O.imol; 0.5 g, 2.4mmol 0.3 mol

RT, overnight 25 ⁰C, 2 h

35:65 35:65

0.108g, 41%, white solid 0.1 g, 25%, yellow solid δ 1.76 (d, 3H, J=6.4 Hz), 6.01 (q, 1 H, J=6.4

Hz (o-coupling), 7.06 (dt, 1 H, J=8.8 Hz (o- δ 2.98 (tt, 4H, J=4.8 Hz)₁ 5.48 (s, 2H), 7.08-7.15 coupling), 7.34 (dt, 1 H, J=9.2 Hz (o-coupling,

(m, 1 H), 7.17-7.28 (m, 4H), 7.33-7.38 (m, 2H),

5.2 Hz), 7.52 (dd, 1H, J=7.2 Hz (o-coupling),

7.87 (broad s, 1H), 8.16 (broad s, 1H)

7.89 (broad s, 1 H), 8.11 (s, 1 H), 8.17 (d, 2H, J=8.4 Hz (o-coupling)

375.14 369.06 5, 15.84 5, 10.5

1H, J=7.6 7.23- s, 1H),

1H,

J=8.4 Hz (o- Hz), (d, 1H,

mmol;

6.97 1H, Hz), 7.39-7.43

Example 100: 3-[4-(2-Bromo-5-methoxy-phenyl)-thiazol-2-ylmethoxy]-2,6- difluoro-benzamide

To a solution of 3-[5-Bromo-4-(2-bromo-5-methoxy-phenyl)-thiazol-2-ylmethoxy]-2,6- difluoro-benzamide (0.06 g, .0001 mol) in 6 ml of acetic acid was added zinc (0.06 g, .0001 mol). The reaction mixture was refluxed for 30 min. The reaction mixture was allowed to come at 25°C. The reaction mixture was filtered on celite bed; the product was precipitated by adding water to the filtrate. The white solid was filtered and dried (0.006 g, 12%). ¹H NMR (DMSO, 400 MHz), 3.79 (s, 3H), 5.59 (s, 2H), 6.94 (dd, 1H, J=8.8 Hz (o-coupling), J=4.0 Hz), 7.09-7.15 (m, 1 H), 7.27 (d, 1 H, J-4.0 Hz), 7.40-7.43 (m, 1H), 7.63 (d, 1H, J=8.8 Hz (o-coupling), 7.89 (broad s, 1H), 8.11 (s, 1H), 8.18 (s, 1H); MS ES+ (455.08 & 457.08). HPLC (method 5) Rt = 10.21 min. Example 118: 2,6-Difluoro-3-[4-(4-methoxy-phenyl)-5-propyl-thiazol-2- ylmethoxyj-benzamide

To a solution of 3-[5-allyl-4-(4-methoxy-phenyl)-thiazol-2-ylmethoxy]-2,6-difluoro- benzamide (0.1 g, 0.02 mmol) in 5 ml of anhydrous methanol was added dry 50 mg of dry Pd-C. The reaction mixture was stirred at 25 ⁰C for 12 h under hydrogen atmosphere. The reaction mixture was filtered over the bed of celite. The filtrate was evaporated to dryness under reduced pressure and the residue was purified by column chromatography on silica (230-400μ) using ethyl acetate/hexane (50:50) as the eluent to provide the title compound as white solid (0.02 g, 2%). ¹H NMR (DMSO- d_β, 400 MHz); δ 0.92 (t, 3H, J=7.2 Hz), 1.63-1.65 (m, 2H), 2.8 (t, 2H, J=7.6 Hz (o- coupling), 3.79 (s, 3H), 5.47 (s, 2H), 7.02 (d, 2H, J=8.8 Hz (o-coupling), 7.11 (m, 1H), 7.42 (m, 1 H), 7.53 (d, 2H₁ J=8.8 Hz (o-coupling), 7.88 (s, 1 H), 8.16 (s, 1 H), 8.38 (d, 1H, J=8.4 Hz (o-coupling). MS ES+ (419.14), HPLC (method 5) Rt=16.58 min.

Example 120: 3-r5-Allvl-4-(4-methoxv-phenvl)-thiazol-2-vlmethoxv1-2,6-difluoro- benzamide

To a solution of 3-[5-bromo-4-(4-methoxy-phenyl)-thiazol-2-ylmethoxy]-2,6- difluoro-benzamide (0.1 g, 0.0002 mol) in 5 ml of anhydrous DMF was added AIIyI tributyltin (0.072 g, 0.0002 mol) and degassed the reaction mixture for the 10 minutes. Then added tetraphenylphosphine Palladium (0) (0.025 g, 0.00002 mol). The reaction mixture was heated at 120⁰C for 12 h under the nitrogen atmosphere. Then reaction mixture was cooled to rt. 100 ml of water was added into it and extracted the compound with ethyl acetate, The combined organic layers were dried over anhydrous Na₂SO₄, and evaporated to dryness under reduced pressure. The compound was purified by column chromatography on silica (230-400μ) using methanol/DCM (2:98) as the eluent to provide the title compound as brown solid (0.120 g, 60%). ¹H NMR (DMSO-d₆, 400 MHz): δ 3.79 (s, 3H), 5.11-5.14 (m, 1H), 5.16 (S, 1 H), 5.48 (s, 2H), 5.57 (s, 1 H), 5.99-6.06 (m, 1 H), 7.03 (d, 2H, J=8A Hz (o- coupling), 7.11 (dt, 1H, J=9.2 Hz (o-coupling), 7.36-7.42 (m, 1 H), 7.56 (d, 2H, J=8.8 Hz (o-coupling), 7.88 (broad s, 1H), 8.16 (broad s, 1H). MS ES+ (417.06), HPLC (method 5) Rt= 16.96 min.

Example 121 : 2,6-Difluoro-3-r4-(4-methoxv-phenyl)-5-pvridin-3-vl-thiazol-2- ylmethoxyj-benzamide

To a solution of 3-[5-bromo-4-(4-methoxy-phenyl)-thiazol-2-ylmethoxy]-2,6- difluoro-benzamide (0.1 g, 0.02 mmol) in DMF: H₂O (2:1), 3-pyridine boronic acid (0.054 g, 0.04 mmol), potassium phosphate (0.056 g, 0.025 mmol) was added. The reaction mixture was degassed for 10 min and then dichlorobis [(triphenylphosphine)- Palladium (II) (0.023 g, 0.003 mmol) was added and again degassed for 10 min. The reaction mixture was heated at 120⁰C for 12 h under the nitrogen atmosphere. DMF was distilled off, after cooling to r.t., water was added into reaction mixture and extracted with ethyl acetate, The combined organic layers were dried over anhydrous Na₂SO₄, and evaporated to dryness under reduced pressure. The compound was purified by chromatography on silica (230-400μ) using ethyl acetate/hexane (50:50) as the eluent to provide the title compound as yellow solid (0.050 g, 50%). ¹H NMR (DMSO-de, 400 MHz): δ 3.75 (s, 3H), 5.59 (s, 2H), 6.92 (d, 2H, J=8.8 Hz (o-coupling), 7.14 (dt, 1 H, J=9.2 Hz (O-coupling), 7.36 (d, 2H, J=8.4 Hz (o-coupling), 7.45 (dt, 2H, J=9.2 Hz (o-coupling) J=5.2 Hz (o-coupling), 7.79 (m, 1H), 7.88 (broad s, 1 H), 8.16 (broad s, 1H), 8.53 (d, 1H, J=2.0 Hz (m-coupiing), 8.57 (d, 1H, J=4.8 Hz). MS ES+ (454.10), HPLC (method 5) Rt= 15.26 min.

Example 123: 3-(5-Bromo-benzothiazol-2-vlmethoxv)-2,6-dif luoro-benzamide

E CONH₂

To a solution of 5-bromo-2-bromomethyl-benzothiazoIe (1.1 g, 0.358 mmol) in 5 ml of anhydrous DMF was added 2,6-difluoro-3-hydroxybenzamide (0.620 g, 0.22 mol) and potassium carbonate (1.73 g, 1.25 mmol). The reaction mixture was stirred at 25°C for 24 h under nitrogen atmosphere. Water was added to the reaction mixture the compound was precipitated out, filtered and washed with diethylether to give the title compound as yellow solid (1.1 g, 76%). ¹H NMR (DMSOd₆, 400 MHz): δ 5.71 (s, 2H), 7.11 (dt, 1H J=8.8 Hz (o-coupling), 7.38-7.39 (m, 1 H), 7.65 (d, 1H, J=8.8 Hz (o-coupling), 7.90 (broad s, 1 H), 8.13 (d, 1 H, J=8.8 Hz (o-coupling), 8.18 (s, 1H), 8.26 (broad s, 1 H). MS ES+ (400.9), HPLC (method 5) Rt= 16.57 min.

Example 125: 2,6-Difluoro-3-r4-(4-methoxv-phenvl)-5-pyridin-2-vl-thiazol-2- ylmethoxyj-benzamide

To a solution of 3-[5-bromo-4-(4-methoxy-phenyl)-thiazol-2-ylmethoxy]-2,6- difluoro-benzamide (0.1 g, 0.02 mmol) in 5 ml of anhydrous DMF 2-tributylstannyl pyridine (0.081 g, 0.02 mmol) was added and degassed for the 10 min. Tetrakis (triphenylphosphine) Palladium (0) (0.026 g, 0.002 mmol) was added to the reaction mixture and again degassed for 10 min. and then heated at 120⁰C for 12 h under the nitrogen atmosphere. Then reaction mixture was cooled to r.t. water was added and extracted with ethyl acetate, The combined organic layers were dried over anhydrous Na₂SO₄, and evaporated to dryness under reduced pressure. The compound was purified by column chromatography on silica (230-400μ) using ethyl acetate (40:60) as the eluent to provide the title compound as white solid (0.120 g, 60%). ¹H NMR (DMSO-d₆, 400 MHz): δ 3.80 (s, 3H), 5.55 (s, 2H), 6.99 (d, 2H, J=8.8 Hz (o-coupling), 7.12 (dt, 1 H₁ J=8.8 Hz (o-coupling), 7.23 (d, 1H, J=8.0 Hz (o-coupling), 7.29-7.32 (m, 1 H), 7.44 (d, 2H₁ J=8.8 Hz (o-coupling), 7.62 (m, 1 H), 7.69 (dt, 1 H, J=8.0 Hz (o- coupling), 7.88 (broad s, 1H), 8.17 (broad s, 1H), 8.60 (d, 1 H, J=4.0 Hz), MS ES+ (454.18), HPLC (method 5) Rt=15.6 min.

Example 126: 3-(5-Allvl-benzothiazol-2-ylmethoxy)-2,6-dif luoro-benzamide

To a solution of 3-(5-bromo-benzothiazol-2-ylmethoxy)-2,6-difluoro-benzamide (0.1 g, 0.025 mol) in 5 ml of anhydrous DMF was added AIIyI tributyltin (0.083 g, 0.025 mol) and degassed the reaction mixture for the 10 minutes. Tetrakis (triphenylphosphine) Palladium (0) (0.029 g, 0.0025 mol) was added and again degassed for 10 min. The reaction mixture was heated at 120⁰C for 1 h under the nitrogen atmosphere, then cooled to r.t. Water was added to the reaction mixture and extracted with ethyl acetate, The combined organic layers were dried over anhydrous Na₂SO₄, and evaporated to dryness under reduced pressure. The compound was crystallized with ethyl acetate/hexane to give the title compound as brown solid (0.050 g, 55%). ¹H NMR (DMSO-d₆, 400 MHz): δ 3.52 (d, 2H, J=6.4 Hz), 5.07-5.13 (m, 1 H) 5.68 (s, 2H) 5.98-6.05 (m, 1 H), 7.10 (dt, 1 H, J=8.4 Hz (o-coupling), 7.31 (d, 1 H, J=8.4 Hz (o-coupling), 7.38 (dt, 1H, J=9.2 Hz (o-coupling), J=5.2 Hz), 7.83 (s, 1H), 7.89 (broad s, 1H), 8.05 (d, 1H, J=8.4 Hz (o-coupling), 8.17 (broad s, 1H) MS ES+ (361.05), HPLC (method 5) Rt= 16.74 min.

Example 127: 2,6-Difluoro-3-[4-(4-methoxy-phenyl)-5-pyridin-4-yl-thiazol-2- ylmethoxy]-benzamide

To a solution of 3-[5-bromo-4-(4-methoxy-phenyl)-thiazol-2-ylmethoxy]-2,6- difluoro-benzamide (0.2 g, 0.43 mmol) in 5 ml of anhydrous DMFiH₂O (2:1) 4- pyridine boronic acid (0.108 g, 0.87 mmol), potassium phosphate ( 0.112 g, 0.51 mmol) was added. Then degassed the reaction mixture for the 10 minutes, and added dichlorobis [(triphenylphosphine)-palladium (II) (0.046 g, 0.06 mmol) and again degassed for 10 min. The reaction mixture was heated at 120⁰C for 12 h under the nitrogen atmosphere. DMF was distilled off, after cooling to r.t. water was added into reaction mixture and extracted with ethyl acetate, The combined organic layers were dried over anhydrous Na₂SO₄, and evaporated to dryness under reduced pressure. The compound was purified by chromatography on silica (230-400μ) using ethyl acetate/hexane (50:50) as the eluent to provide the title compound as white solid (0.045g, 49%). ¹H NMR (DMSO-d₆, 400 MHz): δ 3.80 (s, 3H), 5.59 (s, 2H), 6.94 (d, 2H, J=8.8 Hz (o-coupling), 7.14 (dt, 1H), 7.34 (d, 1H, J=6.0 Hz (o-coupling), 7.38 (d, 2H, J=8.8 Hz (o-coupling), 7.41-7.45 (m, 1 H), 7.89 (broad s, 1 H), 8.17 (s, 1 H)₁ 8.60 (dd, 1 H) MS ES+ (454.12), HPLC (method 5) Rt = 13.55 min.

Example 135: 2,6-Difluoro-3-(5-propyl-benzothiazol-2-ylmethoxy)-benzamide

To a solution of 3-(5-alIyl-benzothiazol-2-ylmethoxy)-2,6-difIuoro-benzamide (0.1 g, 0.27 mmol) in 5 ml of anhydrous methanol was added to 20 mg of dry Pd-C. The reaction mixture was stirred at 25 ⁰C for 12 h under hydrogen atmosphere. The reaction mixture was filtered over the celite bed. The filtrate was evaporated to dryness under reduced pressure and the compound was crystallized with ethyl acetate/hexane to give the title compound as light yellow solid (0.014g, 14%). ¹H NMR (DMSO-d₆, 400 MHz): δ 0.92 (t, 3H, J=7.2 Hz), 1.62-1.68 (m, 2H), 2.71 , 2H, J=7.2 Hz), 5.67 (s, 2H), 7.12 (dt, 1H, J=8.8 Hz (o-coupling) J=1.6 Hz), 7.32 (d, 1H, J=8.4 (o-coupling), 7.38 (dt, 1 H, J=9.2 Hz (o-coupling), J=5.2 Hz), 7.83 (s, 1 H), 7.89 (broad s, 1 H), 8.01 (d, 1H, J=8.4 Hz (o-coupling). 8.17 (broad s, 1 H). MS ES+ (363.08), HPLC (method 5) Rt=17.64 min.

Example 136: 2,6-Difluoro-3-r5-(3-hydroxv-phenvl)-benzothiazol-2-vlmethoxv1- benzamide

To a suspension of 2,6-difluoro-3-[5-(3-methoxy-phenyl)-benzothiazol-2~ ylmethoxy]-benzamide (0.14 g, 0.3 mmol) in 15 ml of anhydrous DCM was added drop wise boron tribromide (0.493 g, 1.9 mmol) at -78⁰C. The reaction mixture was stirred at -78⁰C for 3 h under nitrogen atmosphere. To the reaction mixture 5 ml of water was added at O⁰C. The compound was extracted with ethyl acetate. The combined organic layers were dried over anhydrous Na₂SO₄, and evaporated to dryness under reduced pressure. The compound was purified by column chromatography on silica (230-400μ) using ethyl acetate (40:60) as the eluent to provide the title compound as yellow solid (0.020 g, 14%). ¹H NMR (DMSOd₆, 400 MHz): δ 5.71 (s, 2H), 6.80 (dd, 1 H₁ J=9.6 Hz (o-coupling), 7.11 (dt, 1 H, J=8.0 Hz (o- coupling), 7.17 (dt, 1 H, J=8.0 Hz (o-coupling), 7.29 (t, 1 H, J=8.0 Hz (o-coupling), 7.39-7.43 (m, 1H), 7.71 (dd, 1H, J=9.6 Hz) 7.89 (broad s, 1H), 8.18-8.22 (m, 2H). MS ES+ (413.01), HPLC (method 5) Rt = 14.95 min

Example 140: 2,6-Difluoro-3-r5-(4-hvdroxv-phenvl)-benzothiazol-2-vlmethoxv1- benzamide

Compound 2,6-difluoro-3-[5-(4-methoxy-phenyl)-benzothiazol-2-ylmethoxy]- benzamide (0.095g, 0.223 mmol) was dissolved in 5 ml of DCM and cooled to - 70⁰C. To this, BBr₃ (0.1 ml 0.156 mmol) was added drop wise. After complete addition, reaction mixture was stirred at r.t. for 30 min. The reaction mixture was quenched with MeOH. Reaction mixture was concentrated and purified by column chromatography to obtain (0.0025g, 3%) compound as white solid. ¹H NMR (DMSO- d_βl 400 MHz); δ 5.70 (s, 2H), 6.88 (d, 1H, J=8.4 Hz (o-coupling), 7.10 (m, 1 H), 7.41 (m, 2H), 7.60 (d, 2H₁J= 8.8 Hz, (o-coupling), 7.71 (d, 2H), 7.89 (broad s, 1H), 8.13- 8.17 (m, 2H), 9.62 (broad s, 1 H); MS ES+ (413.0).

Example 141 : 3-r5-f2-Amino-phenvl)-benzothiazol-2-vlmethoxv1-2.6-difluoro- benzamide

To the solution of compound 3-(5-bromo-benzothiazol-2-ylmethoxy)-2,6-difluoro- benzamide (0.3g 0.755 mmol) in dry DMF:H₂O (5 mL: 2.5 mL), phenylamine-2- boronic acid (0.26Og, 1.5mmol), and K₂CO₃ (0.125g, 0.9 mmol)was added under nitrogen atmosphere at room temperature. After that reaction mixture was degassed for half an hour. Dichlorobis [(triphenylphosphine)-palladium (II) was added to the reaction mixture (0.08Og, 0.113 mmol) and again degassed for half an hour and the reaction mixture was heated at 120⁰C for 2 hrs under nitrogen atmosphere. DMF was distilled off, after cooling to r.t. water was added into reaction mixture and extracted with ethyl acetate, The combined organic layers were dried over anhydrous Na₂SO₄, and evaporated to dryness under reduced pressure. The compound was purified by chromatography on silica (230-400μ) using ethyl acetate/hexane (50:50 as the eluent to provide the title compound as yellow solid (0.025g, 8%). ¹H NMR (DMSO-d₆, 400 MHz); δ 4.86 (broad s, 2H), 5.71 (s, 2H), 6.66 (dt, 1 H, J=8.4 Hz (o-coupling), 6.78 (d, 1 H, J=7.2 Hz (o-coupling), 7.04-7.13 (m, 3H), 7.37-7.44 (m, 1 H), 7.51 (dd, 1 H, J=8.4 Hz (o-coupling), J= 1.6 Hz (m-coupling), 7.89 (broad s, 1H), 8.00 (broad s, 1 H), 8.18 (d, 2H, J= 4.0 Hz); MS ES+ (412.16), HPLC (method 5) Rt = 15.33 min.

Example 143; 2.6-Difluoro-3-r5-(3-methoxv-phenvl)-benzothiazol-2-vlmethoxvl- benzamide

To the solution of compound 3-(5-bromo-benzothiazol-2-ylmethoxy)-2,6-difluoro- benzamide (0.30Og, 0.755mmol) in dry DMF:H₂O (5mL: 2.5 mL), added 3- methoxyphenyl boronic acid (0.228g, 1.5 mmol), and K₃PO₄ (0.19Og, 0.9 mmol) under the inert condition at room temperature and degassed for half an hour. Then to the reaction mixture added dichlorobis [(triphenylphosphine)-palladium (II) (0.078g, 0.075mmol) and again degassed for half an hour. The reaction mixture was heated at 120⁰C for 2 hrs under nitrogen atmosphere. DMF was distilled off, after cooling to r.t. water was added into reaction mixture and extracted with ethyl acetate, The combined organic layers were dried over anhydrous Na₂SO₄, and evaporated to dryness under reduced pressure. The compound was purified by chromatography on silica (230-400μ) using ethyl acetate/hexane (50:50 as the eluent to provide the title compound as white solid (0.14Og, 43%). ¹H NMR (DMSO-d₆, 400 MHz); δ 3.85 (s, 3H), 5.72 (s, 2H), 6.97 (t, 1 H, J=6.8 Hz (o-coupling), 7.11 (t, 1 H, J= 8.8 Hz, (o- coupling), 7.30 (broad s, 1 H), 7.34 (d, 1H, J= 8.8 Hz (o-coupling), 7.40 (dd, 2H, J=8.0 Hz (o-coupling), 7.79 (d, 1 H, J=8.0 Hz (o-coupling), 7.90 (broad s, 1 H), 8.18 (broad s, 1 H), 8.21 (d, 1 H, J= 8.0 Hz); MS ES+ (427.14), HPLC (method 5) Rt = 16.48 min.

Example 155 ^θ-Difluoro-S-μ^-methoxy-phenylH^δ'lbithiazolyl-Z¹- ylmethoxy]-benzamide

To a solution of 3-[5-bromo-4-(4-methoxy-phenyl)-thiazol-2-ylmethoxy]-2,6- difluoro-benzamide (0.1 g, 0.2 mmol) in 5 ml of anhydrous DMF was added 2- tributylstannyl thiazole (0.071 g, 0.2 mmol) and degassed the reaction mixture for the 10 minutes. Then added tetraphenylphosphine palladium (0) (0.026 g, 0.2 mmol). The reaction mixture was heated at 120⁰C for 12 h under the nitrogen atmosphere. Then reaction mixture was cooled to rt. 100 ml of water was added into it and extracted the compound with ethyl acetate, The combined organic layers were dried over anhydrous Na₂SO₄, and evaporated to dryness under reduced pressure. The compound was purified by column chromatography on silica (230-400μ) using ethyl acetate (40:60) as the eluent to provide the title compound as yellow solid (0.003 g, 3%). ¹H NMR (DMSO-d₆, 400 MHz): δ 3.82 (s, 3H), 5.57 (s, 2H), 7.06 (d, 1 H, J=8.4 Hz (o-coupling), 7.13 (dt, 1 H), 7.39-7.47(m, 1 H), 7.51 (d, 2H, J=8.4 Hz (o-coupling), 7.52-7.58 (m, 1 H), 7.59-7.86 (m, 2H), 7.68 (d, 1 H, J=3.2 Hz), 7.84 (d, 1 H, J=3.2 Hz), 7.89 (broad s, 1 H), 8.18 (broad s, 1H), 9.12 (s, 1 H); MS ES+ (460.01), HPLC (method 5) Rt = 15.64 min.

Example 160: 2.6-Difluoro-3-r3-(5-methvl-2-phenvl-thiazol-4-yl)-propoxv1- benzamide

3-(5-Methyl-2-phenyl-thiazol-4-yl)-propan-1-ol

To a solution of 3-(5-methyl-2-phenyl-thiazol-4-yl)-propan-1-ol (0.219g, 1.0 mmol) in 5 ml of anhydrous DMF, 2,6-difluoro-3-hydroxybenzamide (0.173g, 1.0 mmol), PPh₃ (0.262g, 1.0 mmol) and diisopropyl azodicarboxylate (0.202g, 1.0 mmol) was added. The reaction mixture was stirred at 80⁰C for overnight under nitrogen atmosphere. The reaction mixture was evaporated to dryness under reduced pressure and the residue was purified by column chromatography on silica (230- 400μ) using ethyl acetate/hexane (35:65) as the eluent to provide the title compound as white solid (0.05Og, 13%). ¹H NMR (DMSO-d₆, 400 MHz): δ 2.44 (broad s, 3H), 3.14 (t, 2H, J=6.4 Hz), 4.35 (t, 2H, J=6.4 Hz), 7.04 (dt, 1H, J=9.2 Hz (o-coupling), 7.22-7.28 (m, 1 H), 7.43-7.49 (m, 3H), 7.83-7.86 (m, 3H), 8.10 (s, 1H); MS ES+ (375.15), HPLC (method 5) Rt = 10.67 min.

Example 164: 2,6-Difluoro-3-r4-(4-methoxv-phenvlH5,5'1bithiazolyl-2- ylmethoxy]-benzamide

To a solution of 2,6-difluoro-3-[4-(4-methoxy-phenyl)-[5,5']bithiazolyl-2- ylmethoxyj-benzamide (0.10Og, 0.2 mmol) in 5 ml of anhydrous DMF, and 5- Tributylstannanyl-thiazole-2-carboxylic acid (0.091 g,0.2mmol) was added. The reaction mixture was stirred at 80⁰C for overnight under nitrogen atmosphere. The reaction mixture was evaporated to dryness under reduced pressure and the residue was purified by column chromatography on silica (230-400μ) using ethyl acetate/hexane (35:65) as the eluent to provide the title compound as white solid (0.025g, 25%). ¹H NMR (DMSOd₆, 400 MHz): δ 3.78 (s, 3H), 5.57 (s, 2H), 6.97 (d, 2H, J=8.8 Hz (o-coupling), 7.13 (t, 1H), 7.44 (d, J= 8.8, (o-coupling, 3H), 7.89 (broad s, 1 H), 8.05 (s, 1 H), 8.17 (broad s, 1 H), 9.12 (s, 1 H); MS ES+(459.94), HPLC (method 5) Rt = 15.21 min. Example 165: 2-FIuoro-3-Hexoxy-benzamide

To the solution of 2-fluoro-3-Hydroxy-benzamide (0.12 g, 0.774 mmol) in 20 mL DMF 1-bromohexane (0.13 mL, 1.0 mmol), Potassium Carbonate (0.213, 1.4 mmol) was added. The reaction mixture was stirred at 90⁰C for 4 h. DMF was distilled off and the reaction mixture was extracted with EtOAc. The obtained crude compound was purified by column chromatography on silica (230-400μ) using ethyl acetate/hexane (50:50) as the eluent to provide the title compound. (0.05g, 28%). ¹H NMR (DMSO-d₆, 400 MHz with D₂O): δ 0.82-0.99 (m, 3H), 1.10-1.33 (m, 6H), 1.67- 1.71 (m, 2H), 3.99-4.15 (t, 2H, J=8.0 Hz), 7.08-7.24 (m, 2H). MS ES+ (214.33), HPLC (method 6) Rt=11.15 min.

Example 166: 2-Hydroxy-3-Hexoxy-benzamide

A mixture of 2-fluoro-3-Hexoxy-benzamide (0.30 g, 1.2 mmol), copper sulfate (0.10 g, 0.4 mol) copper (0.015g, 0.2 mmol) and NaOH (2.5 ml) was stirred at 100⁰C for 14 hrs. After completion of reaction the reaction mixture was acidified and extracted with EtOAc. The obtained crude compound was purified by column chromatography on silica (230-400μ) using ethyl acetate/hexane (50:50) as the eluent to provide the title compound as yellow (0.15g, 50 %). ¹H NMR (DMSO-d₆, 400 MHz with D₂O): δ 3.9 (s, 3H), 7.11-7.18(m, 2H), 7.53-7.58 (m, 1 H). MS ES+ (229.0 M+2H adduct). HPLC (Method 7) Rt=11.16 min.

Example 167: Synthesis of 3-Fluoro-5-hexyloxy benzamide

To a solution of 3-amino-5-hexyloxy benzamide (0.9 g, 3.8 mmol) in tetrafluoroboric acid (20ml), a solution of sodium nitrite (0.315 mg, 4.6 mmol) in water (5 ml) was added at O⁰C and stirred for 1 hr. Later it was allowed to come to RT and stirred for 1 hr followed by heating at 60⁰C for 2hrs.lt was then basify to pH=14 using saturated NaOH solution and extracted with dichloromethane (3x30ml). The solvent was evaporated to yield crude product, which was purified by column chromatography using silica gel (230-400mesh) and dichloromethane as an eluent (100 mg, 11%). ¹H NMR (DMSOd₆, 400 MHz with D₂O): δ 0.88 (t, J=I.2Hz, 3H), 1.32 (m, 2H), 1.41 (m, 4H), 1.72 (m, 2H), 4.0 (t, J=7.2Hz, 2H), 6.97 (m, 1 H), 7.22 (m, 1 H), 7.28 (m, 1H), 7.52 (br s, 1 H), 8.03(br s, 1 H). MS ES+ (238.0, 239.0), HPLC (method 7) Rt=11.34 min.

Example 168: Synthesis of 3-(Pyrazol-1-ylmethoxy)-benzamide

3-(Pyrazol-1-ylmethoxy)-benzoic acid methyl ester (250 mg, 1.1 eq.) was taken in a pressure vessel along with 5 ml of aq. ammonia, heated at 110⁰C for 12 hr. reaction mass was then poured in water (25 ml), extracted with dichloromethane (25 ml x 4). Organic layer was dried over sodium sulphate and concentrated to obtain crude solid. Product was purified by column chromatography using 80% EtOAc-DCM as an eluent over 230- 400 mesh silica gel. Pure product was obtained as solid powder (50 mg, 19%). ¹H NMR (DMSO-d₆, 400 MHz with D₂O): δ 6.12 (s, 2H), 6.33 (m, 1 H), 7.25 (m, 1 H), 7.37 (m, 1 H), 7.41 (m, 1H), 7.51 (m, 1 H), 7.56 (m, 2H), 7.95 (br s, 1 H), 7.99 (m, 1H). MS ES+ (218.0, 235.0-Ammonium adduct), HPLC (method 7) Rt=9.08 min.

Example 169: 3-r(2-Methvlcvclopropyl)methoxv1benzenecarboxamide.

Synthesised according to Method C, scheme 3. Yield 27%, mp 119-121⁰C, HPLC- MS (method 1): m/z 206 [M+H]⁺, Rt = 3.47 min.

Example 170: 3-[(5-Methyl-3-pyridinyl)methoxy]benzenecarboxamide.

N-Bromosuccinimide (2.13 g, 12 mmol) and subsequently α.α'-azoisobutyronitriie (16 mg, 0.1 mmol) were added to a solution of 3,5-lutidine (1.14 ml, 10 mmol) in CCI₄ (40 ml). The reaction mixture was stirred at reflux for 2 hrs. After cooling, succinimide was removed by filtration and the filtrate was evaporated to smaller volume (10 ml). To this filtrate, a mixture of 3-hydroxybenzenecarboxamide (550 mg, 4 mmol) and K₂CO₃ (830 mg, 6 mmol) in DMF (5 ml) was added and the new reaction mixture was stirred at 60°C for 24 h. After diluting with CH₂CI₂ (100 ml), the solution was washed with Na₂CO₃ solution (40 ml) and water (40 ml), dried (Na₂SO₄) and evaporated to dryness, under reduced pressure. The brown oil residue was extracted by trituration with Et₂O (2x10 ml), and from the Et₂O extracts, the precipitant solid was filtered and washed with pentane, to give 70 mg (7.2% yield) of the desired product. Mp 152- 154°C, HPLC-MS: m/z 243 [M+H]⁺, Rt = 2.28 min.

Example 171: 3-[(3-Bromobenzyl)oxy]benzenecarboxamide.

Synthesised according to Method B, scheme 2. Yield 54%, mp 129-131⁰C, HPLC-MS (method 1): m/z 347 [M+H+CH₃CN]\ Rt = 3.99 min.

Scheme 20: (a) KOH aq, (CH₃CO)₂O; (b) 3-hydroxybenzenecarboxamide, PPh₃, DIAD, Et₃N, THF, r.t; (c) K₂CO₃, MeOH, H₂O.

3-(Hydroxymethyl)phenyI acetate.

To a stirred solution of 3-hydroxybenzylalcohol (1.0 g, 8 mmol, 1 equiv.) in 6.4N KOH solution (1.86 ml, 12 mmol, 1.5 equiv.) at r.t, ice (4g) was added followed by acetic anhydride (0.95 ml, 10 mmol, 1.25 equiv.). The reaction mixture was stirred at r.t. for 3h. Water (50 ml) was added and the mixture was stirred for 30 min, before extracting with CH₂CI₂ (2x50 ml). The combined organic extracts were washed with brine (50 ml), dried (Na₂SO₄) and evaporated to dryness, under reduced pressure. The clear oil residue was purified by column chromatography on silica, eluted with EtOAc/hexane (1 :2), to give the desired product as a clear oil (714 mg, 54% yield). HPLC-MS (method 1): m/z 165 [M-H]-. Rt = 2.52 min.

Example 172: 3-[3-(Aminocarbonyl)phenoxy]methylphenyl acetate.

Synthesised according to Method C, scheme 3. Yield 32%, HPLC-MS (method 1): m/z 286 [M+H]⁺. Rt = 3.44 min.

Example 173: 3-lϊ3-Hvdroxvbenzvl)oxv1benzenecarboxamide.

A solution of K₂CO₃ (500 mg, 3.62 mmol, 5.75 equiv.) in water (5 ml) was added to a solution of 3-[3-(aminocarbonyl)phenoxy]methylphenyl acetate (180 mg, 0.63 mmol, 1 equiv.) and the mixture was stirred at r.t., under N₂, for 3 h; The mixture was acidified with 10% HCI solution to pH 1, and was extracted with EtOAc (2x30 ml). The combined organic extracts were washed with water (30 ml), dried (Na₂SO₄) and evaporated to dryness under reduced pressure, to give a clear oil residue which, after trituration with Et₂O, solidified to a white solid (70 mg, 46% yield). Mp 122- 123°C, HPLC-MS (method 1): m/z 244 [M+H]⁺. Rt = 2.92 min.

Scheme 21 : (a) Hexanol, 3 equ. NaH, 100-120⁰C; (b) SOCI₂, toluene, reflux; (c) aqueous NH₃.

3-Chloro-2-(hexyloxy)isonicotinic acid.

A solution of sodium hydride (60% in mineral oil, 600 mg, 15.0 mmol, 3 equiv.) in hexanol (10 ml) was stirred at r.t. for 2 h. 2,3-Dichloro-isonicotinic acid (960 mg, 5.0 mmol, 1 equiv.) was added and the reaction mixture was stirred at 100⁰C for 16 h. The mixture was diluted with water (100 ml) and pentane (300 ml), and the two phases were separated. The aqueous phase was neutralised with 1N HCI solution to pH 6.0 and extracted with EtOAc (3x80 ml). The combined EtOAc extracts were dried (MgSO₄) and evaporated under reduced pressure to dryness. The residue was triturated with pentane, cooled at 0⁰C and the precipitant solid was filtered, to give 410 mg of a white compound (yield 32%). By ¹H-NMR analysis, it consisted of about 80% of the desired product, which was used to the next step without further purification. HPLC-MS: m/z 256 [M-H]^", Rt = 2.94 min.

Example 174: 3-Chloro-2-(hexyloxy)isonicotinamide.

Synthesised from 3-chloro-2-(hexyloxy)isonicotinic acid according to Method A.

Yield 85% (crude); purified further by preparative TLC, mp 75-77°C, HPLC-MS: m/z 298 [M+H+CH₃CN]⁺, Rt = 4.16 min. 19

2-fluoro-3-hydroxybenzenecarboxamide.

Synthesised from commercially available 2-fluoro-3-methoxybenzenecarboxamide according to Method H. Yield 82%, mp 196-197°C, HPLC-MS (method 1): m/z 154 [M-H]-, Rt = 1.24 min.

Example 175-178 (Table F)

Examples 175-178 were synthesised from 2-fluoro-3-hydroxybenzenecarboxamide. Examples 175, 176 and 178 according to Method B, scheme 2 and Example 177 according to Method C, scheme 3.

Table of names of product compounds; Examples 175- 178:

Scheme 22: (a) Methyl 4-bromocrotonate, K₂CO₃, DMF, r.t; (b) NaOH, IPA/H₂O, reflux; (c) n-BuBr, K₂CO₃, DMF, 50°C; (d) H₂, 5% Rh/C, BuOH, r.t.

Example 179: Methyl 4-[3-(aminocarbonyl)-2,4-difluorophenoxy]-2-butenoate.

Synthesised from 2,6-difluoro-3-hydroxybenzenecarboxamide according to Method B, scheme 2. Yield 41%, mp 122-123°C, HPLC-MS (method 1): m/z 272 [M+H]⁺, Rt = 2.80 min.

4-[3-(aminocarbonyl)-2,4-difluorophenoxy]-2-butenoic acid.

A solution of methyl 4-[3-(aminocarbonyl)-2,4-difluorophenoxy]-2-butenoate (1.25 g, 4.61 mmol, 1 equiv.) and NaOH (0.75 g, 18.44 mmol, 4 equiv.) in isopropanol (10 ml) and H₂O (20 ml) was heated under reflux for 1 h. After cooling to r.t., the mixture was acidified with cone. HCI to pH 1. The white precipitant solid was filtered and washed with Et₂O (50 ml), to give 568 mg, 48% yield, mp 187-188°C, HPLC-MS (method 1): m/z 258 [M+H]⁺, Rt = 0.98 min. By ¹H-NMR analysis it was determined to be a mixture of isomeres in a ratio (3:2) £:Z. The aqueous phase was extracted with Et₂O (2x50 ml) and the combined extracts were dried (Na₂SO₄) and evaporated to dryness under reduced pressure, to give a light orange solid, 418 mg, 35% yield, mp 127-128°C, HPLC-MS (method 1): m/z 258 [M+H]⁺, Rt = 0.99 min. By ¹H-NMR analysis it was determined to be a mixture of isomeres in a ratio (3:40) BZ.

Example 180: Butyl 4-[3-(aminocarbonyI)-2,4-difluorophenoxy]-2-butenoate.

4-[3-(AminocarbonyI)-2,4-difluorophenoxy]-2-butenoic acid, mixture of isomeres (3:2) BZ, (526 mg, 2 mmol, 1 equiv.) was dissolved in dry DMF (5 ml). K₂CO₃ (850 mg, 6 mmol, 3 equiv.) and n-butylbromide (0.23 ml, 2.1 mmol, 1.05 equiv.) were added and the reaction mixture was heated for 70 h at 50⁰C and for 1.5 h at r.t. After cooling at r.t., the mixture was diluted with H₂O (50 ml) and extracted with EtOAc

(3x40 ml). The combined organic extracts were washed with H₂O (6x30 ml), dried (MgSO₄) and evaporated to dryness under reduced pressure. The oily residue was purified by column chromatography on silica, eluted with CH₂CI₂ and MeOH/CH₂CI₂

(1%), to give 364 mg, 57% yield, mp<40°C. HPLC-MS (method 1): m/z 314 [M+H]⁺,

Rt = 3.88 min. By ¹H-NMR analysis it was determined to be a mixture of isomeres in a ratio (5:7) EZ. When the same reaction was performed on the acid (3:40) EZ mixture of isomeres, the product obtained was determined to be a mixture of isomeres in a ratio (1 :4) EZ.

Example 181 : Butyl 4-[3-(aminocarbonyl)-2,4-difluorophenoxy]butanoate.

4-[3-(Aminocarbonyl)-2,4-difluorophenoxy]-2-butenoic acid (100 mg, 0.32 mmol) was stirred with 5% Rh/C (5 mg) in butanol (5 ml) under H₂, at r.t. for 21 h. The reaction mixture was filtered through a pad of celite and rinsed with CH₂CI₂ (3x5 ml). The filtrate was evaporated to dryness, under reduced pressure, to give 88 mg of the desired product, yield 87%, mp 53-55°C. HPLC-MS (method 1): m/z 316 [M+H]⁺, Rt = 3.49 min. Example 182-197 (Table G)

Examples 182-197 were synthesised from 2,6-difluoro-3- hydroxybenzenecarboxamide: Examples 182, 190, 192, 193 and 195 according to according to Method B, scheme 2 and Examples 183-189, 191 , 194 and 196-197 according to Method C, scheme 3.

Table of names of product compounds; Examples 182-197:

Scheme 23: (a) n-BuLi, Et₂NH, THF; (b) NaIO₄, MeOH; (c) NaBH4, MeOH; (d) PPh₃, DIAD, Et₃N, THF, r.t

Example 198: 2,6-Difluoro-3-[(2-methyI-4- pyridinyl)methoxy]benzenecarboxamide.

Synthesised from 2,6-difluoro-3-hydroxybenzenecarboxamide according to Method C, scheme 3. The required building block, 4-hydroxymethyl-2- methylpyridine, was synthesised according to the literature method, shown in Scheme 23 (Ragan, JA, Jones, B.P., Meltz, CN, Teixeira J.J. Jr.; Synthesis 2002, 483-486. Yield 34%, mp 185-186°C, HPLC-MS (method 1): m/z 279 [M+H]⁺, Rt = 2.50 min.

Example 199: 2,6-Difluoro-3-([1 ,3]oxazolo[4,5-ϋ]pyridin-2- ylmethoxy)benzenecarboxamide.

Synthesised from 2,6-difluoro-3-hydroxybenzenecarboxamide according to Method B, scheme 2. Yield 8%, mp 180-181⁰C, HPLC-MS (method 1): m/z 306 [M+H]⁺, Rt = 2.30 min.

Example 200: 2,6-Difluoro-3-(2-quinoIinylmethoxy)benzenecarboxamide.

Synthesised from 2,6-difluoro-3-hydroxybenzenecarboxamide according to Method B, scheme 2. Yield 48%, mp 216-218°C, HPLC-MS (method 1): m/z 315 [M+H]⁺, Rt = 3.43 min.

Example 201: 3-(1 -Benzothiophen-5-ylmethoxy)-2,6- difluorobenzenecarboxamide.

Synthesised from 2,6-difluoro-3-hydroxybenzenecarboxamide according to Method B, scheme 2. The required building block, 5-(chloromethyl)-1- benzothiophene, was synthesised by chlorination of commercially available 1- benzothiophen-5-ylmethanol with thionyl chloride. Yield 10%, mp 146-148°C, HPLC- MS (method 1): m/z 320 [M+H]⁺, Rt = 3.95 min.

Examples 202-207 (Table H) Examples 202-207 were synthesised from 2,6- difluoro-3- hydroxybenzenecarboxamide according to Method C, scheme 3.

Table of names of product compounds; Examples 202-207:

[5-(Trifluoromethyl)-1-benzothiophen-2-yl]methanol.

Pyridine (0.37 ml, 4.72 mmol, 1.5 equiv.) and subsequently cyanuric fluoride (0.53 ml, 6.3 mmol, 2 equiv.) were added to a stirred solution of commercially available 5- (trifluoromethyl)-1-benzothiophene-2-carboxylic acid (776 mg, 3.15 mmol, 1 equiv.) in CH₂CI₂ (16 ml), kept under N₂, at -20 to -10⁰C. Precipitation of cyanuric acid occurred and increased gradually as the reaction proceeded. After the mixture was stirred at -20 to -10⁰C for 2 h, ice-cold water was added along with 100 ml CH₂CI₂. Undissolved solids were filtered off; from the filtrate, the organic phase was separated and the aqueous layer was extracted once more with CH₂CI₂ (50 ml). The combined organic layers were washed with ice-cold water (50 ml), dried (Na₂SO₄) and concentrated under reduced pressure to a small volume (15 ml). NaBH₄ (240 mg, 6.3 mmol, 2 equiv.) was added in one portion, and MeOH (6.5 ml) was then added, dropwise, over 15 min at r.t. The reaction mixture was neutralised with 1N H₂SO₄, and the organic solvents were evaporated under reduced pressure. The residue was taken-up in EtOAc (80 ml) and water (40 ml); the organic layer was separated, and the aqueous layer was extracted with EtOAc (2x60 ml). The combined organic layers were washed with 1N H₂SO₄ and brine, dried (Na₂SO₄) and the solvent was evaporated under reduced pressure. The residue was purified by column chromatography on silica, using EtOAc/hexane (10-20% gradient) as eluent, to give 400 mg (54.6% yield) of the required product as a white solid. HPLC-MS (method 1) gave one peak with Rt = 4.02 min, but no ionization.

Example 208: 2,6-Difluoro-3-[5-(trif luoromethyl)-1 -benzothiophen-2- yl]methoxybenzenecarboxamide.

Synthesised from 2,6-difluoro-3-hydroxybenzenecarboxamide and [5- (trifluoromethyl)-1-benzothiophen-2-yl]methanol according to Method C, scheme 3. Yield 3%, mp 150-152⁰C₁ HPLC-MS (method 1): m/z 386 [M-H]^", Rt = 4.39 min.

Examples 209-217 (Table I) Examples 209-217 were synthesised from 2,6- difluoro-3- hydroxybenzenecarboxamide according to Method B, scheme 2.

I 212 I I 213 I I 214 I

Table of names of product compounds; Examples 209-217:

5-Chloro-2-(chloromethyl)-1,3-benzothiazole.

4-Chloro-2-amino-benzothiol (4.05 g, 25.4 mmol, 1 equiv.) and 2-chloro-1 ,1 ,1- trimethoxy ethane (5.0 ml, 37 mmol, 1.45 equiv.) were heated with stirring at 60°C for 2 h. The reaction mixture was cooled at r.t. and triturated with diethyl ether (10 ml). The undissolved solid was filtered and rinsed with Et₂O and pentane, to give 1.54 g (28% yield) of the desired product. The mother liquors were evaporated to dryness, the orange solid residue was dissolved in Et₂O (50 ml) and washed consecutively with 1 N HCI (25 ml), water (25 ml), 5% NaHCO₃ solution (25 ml) and brine (25 ml). The organic layer was dried (MgSO4) and evaporated to smaller volume, under reduced pressure. The precipitant solid was filtered and washed with Et₂O and pentane, to give a second fraction of the desired product 1.88 g (34% yield). Total yield 62%, mp 102-104⁰C, HPLC-MS (method 1): m/z 260 [M+H+CH₃CN]⁺, Rt = 4.52 min.

Examples 218-221 (Table J)

Examples 218-221 were synthesised from 2,6-difluoro-3- hydroxybenzenecarboxamide and 5-chloro-2-(chloromethyl)-1 ,3-benzothiazole according to Method B, scheme 2.

Table of names of product compounds; Examples 218-221 :

Example Compound name

218 3-[(5-Chloro-1,3-benzothiazol-2-yl)methoxy]-2,6-difluorobenzenecarboxamide

219 3-[(5-Chloro-1 ,3-benzothiazol-2-yl)methoxy]-2-fluorobenzenecarboxamide

220 6-Chloro-3-[(5-chloro-1 ,3-benzothiazol-2-yl)methoxy]-2-fluorobenzenecarboxamide

221 2-Chloro-3-[(5-chloro-1 ,3-benzothiazol-2-yl)methoxy]-6-fluorobenzenecarboxamide Scheme 24: (a) K₂CO₃, NaI, DMF, 60⁰C; (b) cone. H₂SO₄, H₂O, 40⁰C.

2-[(5-Chloro-1,3-benzothiazol-2-yl)methoxy]isonicotinonitrile.

2-Hydroxy-4-cyano-pyridine (240 mg, 2 mmol, 1 equiv.) was dissolved in DMF (6 ml), K₂CO₃ (415 mg, 3 mmol, 1.5 equiv.) and NaI (60 mg, 0.4 mmol, 0.2 equiv.) were added and the mixture was stirred at r.t. for 10 min. 5-Chloro-2-(chloromethyI)-1,3- benzothiazole (436 mg, 2 mmol, 1 equiv.) was added and the reaction mixture was stirred at 60⁰C for 3 h and at r.t. overnight. By addition of H₂O, brown solid precipitated, which was filtered, rinsed with H₂O, dried and re-crystallised from CH₃CN. Yield 280 mg (46%), mp 224-227°C, HPLC-MS (method 1): m/z 302 [M+H]⁺, Rt = 3.80 min. By ¹³C-NMR analysis it was identified to be the N-alkylated derivative (Scheme 24). The DMF-H₂O mother liquors were evaporated to dryness under reduced pressure and the residue was purified by column chromatography on silica, eluted with EtOAc/hexane (10%-100% gradient) to give 45 mg (7.5% yield) of a brown solid, HPLC-MS (method 1): m/z 302 [M+H]⁺, Rt = 4.86 min. By ¹³C-NMR analysis, it was identified to be the desired O-alkylated derivative (Scheme 24).

Example 222: 2-[(5-Chloro-1 ,3-benzothiazol-2-yl)methoxy]isonicotinamide.

2-[(5-Chloro-1,3-benzothiazol-2-yl)methoxy]isonicotinonitrile (40 mg, 0.13 mmol) was dissolved in cone. H₂SO₄ (0.36 ml) and the solution was heated at 40⁰C, under vigorous stirring. Water (50 mg) was added dropwise and the mixture was stirred at 40⁰C for 3 h. After cooling at -5°C, crushed ice (25 ml) was added quickly, with vigorous stirring, and the mixture was stirred at r.t. for two more hours. Ammonia solution was added (pH 10) and the precipitant solid was filtered, rinsed with H₂O and dried. The brown solid was purified by preparative TLC, eluted with EtOAc, to give 20 mg (47% yield), mp 220-222⁰C, HPLC-MS (method 1): m/z 320 [M+H]⁺, Rt = 3.76 min.

Scheme 25: (a) KOH, 2-methoxy-ethanol:H₂O (1 :1), reflux; (b) CICH₂C(OCH₃)₃; (c) K₂CO₃, NaI, DMF, 60⁰C.

2-(Chloromethyl)-4-ethyl-1,3-benzothiazole.

(Method J) A solution of 4-ethyl-1 ,3-benzothiazol-2-amine (1.0 g, 5.6 mmol, 1 equiv.) and KOH (7.4 g, 112.2 mmol, 20 equiv.) in 2-methoxy-ethanol (9 ml) and H₂O (9 ml), was stirred under N₂ and under reflux, for 20 h. After cooling at r.t., the mixture was poured into water (150 ml) and extracted with CH₂CI₂ (2x40 ml). The aqueous phase was neutralised with cone. HCI and extracted again with CH₂CI₂ (3x70 ml). The combined neutral extracts were washed with water (2x60 ml), dried (Na₂SO₄) and evaporated to dryness under reduced pressure. The yellow-green semi-solid residue (790 mg) was mixed with 2-chloro-1,1,1-trimethoxy ethane (1.62 g, 10.4 mmol) and the mixture was stirred, under N₂, at 6O⁰C, for 4 h. Volatiles were removed by evaporation under reduced pressure and the brown liquid residue was purified by column chromatography on silica, eluted with CH₂CI₂/hexane (10% and 50%), to give a yellow liquid (406 mg, 34% yield over two steps). HPLC-MS (method 1): m/z 212 [M+H]⁺, Rt = 5.00 min

Example 223: 3-[(4-Ethyl-1 ,3-benzothiazol-2-yl)methoxy]-2,6- difluorobenzenecarboxamide.

Synthesised from 2,6-difluoro-3-hydroxybenzenecarboxamide and 2- (chloromethyI)-4-ethyl-1,3-benzothiazole according to Method B, scheme 2. Yield 17%, mp 184-186°C, HPLC-MS (method 1): m/z 349 [M+H]\ Rt = 4.16 min.

2-(Chloromethyl)-6-methoxy-1,3-benzothiazole

Synthesised from commercially available 6-methoxy-1 ,3-benzothiazol-2-amine according to Method J, scheme 25. It was used crude on the next step.

Example 224: 2,6-Difluoro-3-[(6-methoxy-1 ,3-benzothiazol-2- yl)methoxy]benzenecarboxamide.

Synthesised from 2,6-difluoro-3-hydroxybenzenecarboxamide and 2- (chloromethyl)-6-methoxy-1,3-benzothiazole according to Method B, scheme 2. Yield 19%, mp 190-192⁰C, HPLC-MS (method 1): m/z 351 [M+H]⁺, Rt = 3.50 min. Scheme 26: (a) BrCH₂CN, K₂CO₃, NaI, DMF, 60⁰C; (b) KOH, 2-methoxy-ethanol:H₂O (1 :1), reflux.

Example 225: 3-(Cvanomethoxv)-2,6-difluorobenzenecarboxamide.

Synthesised from 2,6-difluoro-3-hydroxybenzenecarboxamide according to Method B, scheme 2. Yield 86%, mp 122-123°C, HPLC-MS (method 1): m/z 213 [M+H]\ Rt = 1.97 min.

Example 226: 3-r(4-Chloro-1,3-benzothiazol-2-yl)methoxv1-2,6- difluorobenzenecarboxamide.

(Method K) A solution of KOH (15.15 g, 270 mmol, 20 equiv.) in H₂O (25 ml) was added to a solution of 4-chloro-1 ,3-benzothiazol-2-amine (2.5 g, 13.5 mmol, 1 equiv.) in 2-methoxy-ethanol (25 ml) and the reaction mixture was heated under reflux overnight. After cooling at r.t., the mixture was diluted with H₂O (200 ml), acidified with 5N HCI solution to pH 4 and extracted with CH₂CI₂ (3x150 ml). The combined organic extracts were washed with brine (100 ml), dried (Na₂SO₄) and concentrated under reduced pressure to dryness, to give 1.5 g (70% yield). From this crude residue, 167 mg (assuming 1.05 mmol), were mixed with 3-(cyanomethoxy)-2,6- difluorobenzenecarboxamide (150 mg, 0.7 mmol) and the mixture was stirred at 12O⁰C, in a pre-heated oil bath, under N₂, for 2 h. EtOH (2 ml) was added and the reaction mixture was heated for a further 2 h. After cooling at r.t., the solid was filtered, washed with EtOH and re-crystalised from EtOAc/pentane, to give the desired product as a pale yellow solid, 62 mg (25% yield on second step). HPLC-MS (method 1): m/z 355 [M+H]⁺, Rt = 3.75 min.

Example 227: 3-[(6-Chloro-1 ,3-benzothiazol-2-yl)methoxy]-2,6- difluorobenzenecarboxamide.

Synthesised from 6-chloro-1 ,3-benzothiazol-2-amine and 3-(cyanomethoxy)-2,6- difluorobenzene carboxamide, according to Method K, scheme 26. Yield 38% (second step), mp 190-191⁰C, HPLC-MS (method 1): m/z 355 [M+H]\ Rt = 3.85 min.

Example 228: 2,6-Difluoro-3-[(4-methyI-1 ,3-benzothiazol-2- yl)methoxy]benzenecarboxamide.

Synthesised from 4-methyI-1 ,3-benzothiazol-2-amine and 3-(cyanomethoxy)-2,6- difluorobenzene carboxamide, according to Method K, scheme 26. Yield 36% (second step), mp 201-202⁰C, HPLC-MS (method 1): m/z 335 [M+H]⁺, Rt = 3.79 min.

Example 229: 2,6-Dif luoro-3-[(6-methyl-1 ,3-benzothiazol-2- yl)methoxy]benzenecarboxamide.

Synthesised from 6-methyl-1 ,3-benzothiazol-2-amine and 3-(cyanomethoxy)-2,6- difluorobenzene carboxamide, according to Method K, scheme 26. Yield 17% (second step), HPLC-MS (method 1): m/z 335 [M+H]⁺, Rt = 3.70 min.

Example 230: 2,6-Difluoro-3-[6-(trifluoromethoxy)-1 ,3-benzothiazol-2- yl]methoxybenzenecarboxamide.

Synthesised from 6-(trifluoromethoxy)-1 ,3-benzothiazol-2-amine and 3- (cyanomethoxy)-2,6-difluorobenzenecarboxamide, according to Method K, scheme 26. Yield 34% (second step), mp 174-175°C, HPLC-MS (method 1): m/z 405 [M+H]⁺, Rt = 4.14 min.

6-PropyI-1,3-benzothiazol-2-amine.

A solution of Br₂ (3.8 ml, 74 mmol, 2 equiv.) in glacial AcOH (18.5 ml) was added dropwise, at <25°C, to a stirred solution of 4-propylamine (5.0 g, 37 mmol, 1 equiv.) and ammonium thiocyanate (5.63 g, 74 mmol, 2 equiv.) in glacial AcOH (110 ml). The resulting mixture was stirred at r.t. for 2 h, diluted with H₂O (700 ml) and extracted with EtOAc (2x250 ml). The aqueous layer was alkalised with aqueous ammonia solution to pH 10 and extracted with EtOAc (3x300 ml). The combined alkaline extracts were washed with H₂O (2x200 ml), dried and evaporated to dryness under reduced pressure, to give the desired product as a white solid, 2.34 g (33% yield), mp 120-122⁰C. HPLC-MS (method 1): m/z 193 [M+H]⁺, Rt = 3.92 min. Example 231: 2,6-Difluoro-3-[(6-propvl-1.3-benzothiazol-2- yl)methoxy]benzenecarboxamide.

Synthesised from 6-propyl-1,3-benzothiazol-2-amine and 3-(cyanomethoxy)-2,6- difluorobenzenecarboxamide, according to Method K, scheme 26. Yield 18% (second step), mp 173-175°C. HPLC-MS (method 1): m/z 363 [M+H]⁺, Rt = 4.35 min.

Scheme 27: (a) NBS, α,α'-azoisobutyronitrile, CCI₄; (b) K₂CO₃, DMF, 60⁰C; (c) 4- pyridine boronic acid, Na₂CO₃, Pd(PPh₃)₄, dioxane.

5-Bromo-2-(bromomethyl)-1 ,3-benzothiazole.

N-Bromosuccinimide (4.45 g, 25 mmol, 1.4 equiv.) and subsequently α,α'~ azoisobutyronitrile (110 mg, 0.7 mmol, 0.04 equiv.) were added to a solution of 5- bromo-2-methyl-benzothiazole (4.07 g, 17.85 mmol, 1 equiv.) in CCI₄ (110 ml). The reaction mixture was stirred at reflux for 24 hrs. After cooling, succinimide was removed by filtration and was rinsed with CCI₄ (100 ml). The filtrate was evaporated to dryness under reduced pressure and the orange solid residue was purified by column chromatography on silica, eluted with CH₂CI₂/hexane (20%-70% gradient), to give the desired product as a white solid, 2.15 g (39% yield). Mp 116-117, HPLC-MS (method 1): m/z 308 [M+H]⁺, Rt = 4.84 min. The reaction gave also 1.40 g (20% yield) of the by-product 5-bromo-2-dibromomethyl-benzothiazole, as well as 0.89 g (22%) of un-reacted starting material.

3-[(5-Bromo-1,3-benzothiazoI-2-yl)methoxy]-2,6-difluorobenzenecarboxamide.

Synthesised from 2,6-difluoro-3-hydroxybenzenecarboxamide and 5-bromo-2- (bromomethyl)-1,3-benzothiazole, according to Method B, scheme 2. Yield 81%, mp 244-246°C, HPLC-MS (method 1): m/z 399, 401 [M+H]⁺, Rt = 3.98 min.

Example 232: 2,6-Dif luoro-3-[5-(4-pyιϊdinyl)-1 ,3-benzothiazol-2- yl]methoxybenzenecarboxamide.

A mixture of 3-[(5-bromo-1,3-benzothiazol-2-yl)methoxy]-2,6- difluorobenzenecarboxamide (168 mg, 0.42 mmol, 1 equiv.), 4-pyridine boronic acid (98 mg, 0.63 mmol, 1.5 equiv.) and 2M aqueous Na₂CO₃ solution (0.42 ml, 0.82 mmol, 2 equiv.) were suspended in dioxane (3.5 ml) and the mixture was degassed and flushed with N₂. Tetrakis(triphenylphosphine)palladium(0) catalyst (37 mg, 0.031 mmol, 0.075 equiv.) was added and the reaction mixture was heated under reflux for 12 h. After cooling at r.t., the mixture was diluted with H₂O and the precipitant solid was filtered and rinsed with H₂O, IMS, IMS/Et₂O and Et₂O. Re-crystallised from CH₃CN, to give the desired product as an off-white solid, 47 mg (28% yield), mp 255- 258°C. HPLC-MS: m/z 398 [M+H]⁺, Rt = 3.28 min. Examples 233-241 (Table K)

Examples 233-241 were synthesised from 2,6-difluoro-3- hydroxybenzenecarboxamide according to Method B, scheme 2.

Table of names of product compounds; Examples 233-241:

Example Compound name

233 2,6-Difluoro-3-[(2-phenyl-1 ,3-thiazol-4-yl)methoxy]benzenecarboxamide

234 3-[5-(4-Chlorophenyl)-1 ,3,4-thiadiazol-2-yl]methoxy-2,6-difluorobenzenecarboxamide

Example 242: 2,6-Difluoro-3-r3-(4-hydroxyphenyl)-1 ,2,4-oxadiazol-5- yljmethoxybenzenecarboxamide.

(Method L) Bororn tribromide solution (1.0 M in CH₂CI₂, 1.5 ml, 1.5 mmol, 2 equiv.) was added slowly, dropwise to stirred suspension of 2,6-difluoro-3-[3-(4- methoxyphenyl)-1 ,2,4-oxadiazol-5-yl]methoxybenzenecarboxamide (272 mg, 0.75 mmol, 1 equiv.) in CH₂CI₂ (5 ml), at r.t, under N₂. The reaction mixture was stirred at r.t. for 4 h and poured into water (20 ml). CH₂CI₂ (10 ml) was added and the biphasic mixture was stirred for 30 min. at r.t. The white un-dissolved solid was filtered, washed with water and Et₂O, to give 170 mg (65% yield), mp 209-210⁰C, HPLC-MS (method 1): m/z 348 [M+Hf , Rt = 3.00 min.

Examples 243-250 (Table L) Examples 243-250 were Synthesised from 2,6-difluoro-3- hydroxybenzenecarboxamide according to Method B, scheme 2.

Table of names of product compounds; Examples 243-250:

Scheme 28: (a) (BoC)₂O, Et₃N, DMAP, THF; (b) K₂CO₃, NaI, DMF, r.t; (c) 4N HCI, dioxane, r.t.

(b)

tert-Butyl ΛM-β-fchloromethyO-i^^-oxadiazol-S-yriphenylcarbamate.

To a solution of 4-[5-(chloromethyl)-1 ,2,4-oxadiazol-3-yl]aniline (950 mg, 4.53 mmoi, 1 equiv.), Et₃N (0.20 ml, 5.44 mmol, 1.2 equiv.) and dimethylaminopyridine (catalytic), Boc anhydride (1.04 g, 4.75 mmol, 1.05 equiv.) was added portionwise, and the reaction mixture was stirred at r.t. for 3 days. The solvent was evaporated under reduced pressure, the residue was triturated with Et₂O and the solid was removed by filtration. The filtrate was evaporated to dryness under reduced pressure and the residue was purified by column chromatography on silica, eluted with EtOAc/hexane (20%), to give a cream solid, 780 mg (55% yield). About 70% pure by HPLC-MS (method 1): m/z 308 [M-H]^", Rt = 4.72 min. It was used without further purification on the next step.

tert-Butyl Λ/-[4-(5-[3-(aminocarbonyl)-2,4-difluorophenoxy]methyl-1,2,4- oxadiazol-3-yl)phenyl]carbamate.

Synthesised from 2,6-difluoro-3-hydroxybenzenecarboxamide and teif-butyl tø-4- [5-(chloromethyl)-1,2,4-oxadiazol-3-yl]phenylcarbamate, according to Method B, scheme 2, at r.t. Yield 42%, mp 165-166°C, HPLC-MS (method 1): m/z AAl [M+H]⁺, Rt = 4.10 min.

Example 251: 3-[3-(4-Aminophenyl)-1 ,2,4-oxadiazol-5-yl]methoxy-2,6- difluorobenzenecarboxamide hydrochloride salt.

terf-Butyl /V-[4-(5-[3-(aminocarbonyl)-2,4-difluorophenoxy]methyl-1,2,4- oxadiazol-3-yl)phenyl]carbamate (300 mg, 0.67 mmol, 1 equiv.) was dissolved in 4N HCI in dioxane (7 ml, 28 mmol, 42 equiv.) and the reaction mixture was stirred at r.t. overnight. Volatiles were removed under reduced pressure, the residue was triturated with dry Et₂O and the solid formed was filtered and rinsed with dry Et₂O. The crude product (200 mg) was taken-up in EtOH (2 ml) and was triturated with 2N HCI in Et₂O solution (0.3 ml) and dry Et₂O. The white solid was filtered and washed with dry Et₂O, to give 110 mg of the desired product (43% yield). HPLC-MS (method 1): m/z 347 [M+H-HCI]⁺, Rt = 2.98 min.

Examples 252-266 (Table M)

Examples 252, 254-256 and 258-266 were synthesised from 2,6-difluoro-3- hydroxybenzenecarboxamide according to Method B, scheme 2. Examples 253 and 257 were synthesised from 2,6-difluoro-3-[3-(2-methoxyphenyl)-1 ,2,4-oxadiazoI-5- yl]methoxybenzene carboxamide according to Method L.

Table of names of product compounds; Examples 252-266:

Scheme 29: (a) NH₂OH-HCI, NaOH, EtOH; (b) Bromoacetyl bromide, (c) K₂CO₃, DMF

4- Chloro-N-hydroxy-benzamide

To a solution of 4-chlorobenzonitrile (10.0 g, 73.0 mmol) in EtOH (250 mL) was added hydroxylamine hydrochloride (5.03 g, 73.0 mmol) and NaOH (2.90 g, 73.0 mmol). The resulting reaction mixture was refluxed for 15h. After the completion of the reaction (TLC monitoring), the mixture was concentrated, added EtOH and filtered. The filtrate was evaporated in vacuo and used as such for the next step (crude yield 12.0 g, 66%).

5-Bromomethyl-3-(4-chloro-phenyl)-[1,2,4]oxadiazole

Bromoacetyl bromide (1.50 mL, 17.58 mmol) was added to 4-Chloro-N-hydroxy- benzamide (1.0 g, 5.86 mmol) and K₂CO₃ (3.18 g, 23.44 mmol). The reaction mixture was heated at 100⁰C for 15 min. After the completion of the reaction mixture (TLC monitoring), water (100 mL) was added and extracted with ethyl acetate (3 x 50 mL). The combined organics was washed with water, brine, dried (Na₂SO₄), filtered and concentrated. The crude residue was purified over silica gel (60-120 M, 1% EtOAc- Hexane) to get the desired product (0.44 g, 28%) as a white solid.

Examples 267-270 (Table N) The compounds of Examples 267-270 were synthesised according to the following general procedure: To a solution of 5-bromomethyl-3-(4-chloro-phenyl)- [1 ,2,4]oxadiazole (A) in 2 ml of anhydrous DMF was added reactant (B) and potassium carbonate (C). The reaction mixture was stirred at 25°C for 24 h under nitrogen atmosphere. The reaction mixture was evaporated to dryness under reduced pressure and the residue was purified by column chromatography on silica (230-400 M) using ethyl acetate/hexane (45:55) as the eluent to provide the product compound.

Scheme 30: (a) 2-BenzyIoxy-thioacetamide, DMF; (b) BBr₃, DCM, (c) PBr₃,Toluene (d) corresponding Phenols

H, X= F, Y= H, Z= Cl ; Example 271 H, X= H, Y= F, Z= Cl; Example 272 H, X= F, Y= Cl, Z= Cl; Example 273 H, X= Cl, Y= F, Z= Cl ; Example 274 N, X= H, Y= H, Z= Cl; Example 275

H, X= F, Y= F, Z= CN; Example 276

2-Benzyloxymethyl-4-(4-chloro-phenyI)-thiazole

To the solution of 2-Benzyloxy-thioacetamide (3.Og, 16.57 mmol) in 3 ml of DMF was added 2-Bromo-1-(4-chloro-phenyl)-ethanone (3.0 g, 12.87 mmol). The reaction mixture was heated at 13O⁰C for 24 h under nitrogen atmosphere. After the completion of the reaction mixture (TLC monitoring), water (25 mL) was added and extracted with ethyl acetate (3 x 50 mL). The combined organics was washed with water, brine, dried (Na₂SO₄), filtered and concentrated. The crude residue was purified over silica gel (230-400 M, 2% EtOAc-Hexane) to get the desired product (2.0 g, 49%). The corresponding cyano derivative was also prepared by the same general method.

[4-(4-Chloro-phenyl)-thiazol-2-yl]-methanol

A solution of 2-Benzyloxymethyl-4-(4-chloro-phenyl)-thiazole (2.Og, 6.34 mmol) in 25 ml of DCM was cooled to -78° C followed by addition of BBr₃ (2.38 ml, 25.3 mmol). The reaction mixture was stirred at 25⁰C for 2 h. After the completion of the reaction mixture (TLC monitoring), solution of NaHCO₃ (20 mL) was added at 0° C and extracted with ethyl acetate (3 x 50 mL). The combined organics was washed with water, brine, dried (Na₂SO₄), filtered and concentrated. The crude residue was purified over silica gel (60-120 M, 40% EtOAc-Hexane) to get the desired product (0.8g, 57%). The corresponding cyano derivative was also prepared by the same general method.

2-Bromomethyl-4-(4-chloro-phenyl)-thiazole

To the solution of [4-(4-Chloro-phenyl)-thiazol-2-yI]-methanol (0.8Og₁ 3.55 mmol) in 10 ml of toluene was added PBr₃ (0.51 ml, 5.33 mmol) and the reaction mixture was heated at 12O⁰C for 20 min under nitrogen atmosphere. After the completion of the reaction mixture (TLC monitoring), water (25 mL) was added and extracted with ethyl acetate (3 x 50 mL). The combined organics was washed with water, brine, dried (Na₂SO₄), filtered and concentrated to get the desired product (0.17 g, 17%). The corresponding cyano derivative was also prepared by the same general method.

Examples 271-276 (Table O)

The compounds of Examples 271-276 were synthesised according to the following general procedure: To a solution of reactant (A) in anhydrous DMF was added reactant (B) and potassium carbonate (C). The reaction mixture was stirred at 25°C for 24 h under nitrogen atmosphere. The reaction mixture was evaporated to dryness under reduced pressure and the residue was purified by column chromatography on silica (60-120 M) using ethyl acetate/hexane as the eluent to provide the product compound.

0.95 g,

1 H), 7.40 8.40 Hz,

Scheme 31 : (a) Thioacetamide, DMF; (b) NBS, CCI₄ (c) 2,6-difluoro-3- hydroxybenzamide, K₂CO₃, DMF (d) corresponding boronic acids, Suzuki or Stille conditions (e) CuCN, Pyridine.

R= -! — <d (Example 286) R= H; Example 287 (Example 288)

4-(4-Methoxy-phenyl)-2-methyl-thiazoIe

The mixture of thioacetamide (16.Og, 213 mmol) and 2-bromo-1-(4-methoxy-phenyl)- ethanone (4.0 g, 17.5 mmol) was heated at 14O⁰C for 24 h under nitrogen atmosphere. After the completion of the reaction mixture (TLC monitoring), water (100 ml_) was added and extracted with ethyl acetate (3 x 100 mL). The combined organics was washed with water, brine, dried (Na₂SO₄), filtered and concentrated. The crude residue was purified over silica gel (230-400 M, 1% EtOAc-Hexane) to get the desired product (2.5 g, 69%).

5-Bromo-2-bromomethyl-4-(4-methoxy-phenyl)-thiazole

To the solution 4-(4-Methoxy-phenyl)-2-methyl-thiazole (5.0, 24.3 mmol) in CCI₄ (20 mL) was added NBS (7.43 g, 41.74 mmol) and the reaction mixture was heated at 100⁰C for 2 h under nitrogen atmosphere. After the completion of the reaction mixture (TLC monitoring), the reaction mixture was evaporated to dryness under reduced pressure and the residue was purified by column chromatography on silica (230-400 M) using 1% ethyl acetate/hexane to give the desired product (3.0 g, 34%).

3-[5-Bromo-4-(4-methoxy-phenyl)-thiazol-2-ylmethoxy]-2,6-difluoro-benzamide

To a solution of 5-Bromo-2-bromomethyl-4-(4-methoxy-phenyl)-thiazole (0.50 g, 1.37 mmol) in 5 ml of anhydrous DMF was added 2,6-Difluoro-3-hydroxy-benzamide (0.23 g, 1.37 mmol) and potassium carbonate (0.75 g, 5.43 mmol). The reaction mixture was stirred at 25°C for 24 h under nitrogen atmosphere. The reaction mixture was evaporated to dryness under reduced pressure and the residue was purified by column chromatography on silica (60-120 M) using ethyl acetate/hexane (30:70) as the eluent to provide the title compound (0.30 g, 48%).

Examples 277-287 (Table P) The compounds of Examples 277-287 were synthesised according to the following general procedure: To a solution of 3-[5-bromo-4-(4-methoxy-phenyl)-thiazol-2- ylmethoxy]-2,6-difluoro-benzamide (A) in 5 ml of anhydrous DMF and water (2.5 ml) was added reactant (B) and potassium phosphate (C). The reaction mixture was degassed for 10 minutes followed by addition of dichlorobis(triphenyl phosphine) palladium (II) (D). The reaction mixture was heated at 120⁰C for 12 h under the nitrogen atmosphere. After the completion of the reaction mixture (TLC monitoring), water (25 ml.) was added and extracted with ethyl acetate (3 x 50 ml_). The combined organics was washed with water, brine, dried (Na₂SO₄), filtered and concentrated. The crude residue was purified over silica gel (45% EtOAc-Hexane) to get the desired product compound.

Table P

(m,

0.05 g,

283 284

2,6-Difluoro-3-[5-(3-amino-phenyl)-4-(4- ,6-Difluoro-3-[5-(2-amino-phenyl)-4-(4-methoxy- methoxy-phenyl)-thiazol-2-ylmethoxy]- phenyl)-thiazol-2-ylmethoxy]-benzamide benzamide

2-aminophenyl boronic acid 3-aminophenyl boronic acid

0.10 g, 0.20 mmol; 0.09 g, 0.54 mmol; 0.10 g, 0.10 g, 0.20 mmol; 0.07 g, 0.54 mmol; 0.102 0.48 mmol; 0.021 g, 0.03 mmol g, 0.48 mmol; 0.021 g, 0.03 mmol

60-120 M 60-120 M

0.042 g, 41%, light yellow solid 0.015 g, 14%, light yellow solid δ 3.72 (s, 3H), 4.94 (br s, 2H), 5.54 (s, 2H), 6.57 δ 3.74 (s, 3H), 5.25 (br s, 2H), 5.53 (s, 2H),

(t, J= 7.20 Hz, 1 H), 6.75 (d, J= 8.0 Hz, 1H), 6.84 6.44 (m, 1 H), 6.56 (m, 2H), 6.89 (m, 2H), 7.03

(m, 2H), 7.0 (m, 1 H), 7.14 (m, 2H), 7.51 (m, 3H), (m, 1 H), 7.13 (m, 1 H), 7.42 (m, 3H), 7.88 (br

7.89 (br s, 1 H) and 8.18 (br s, 1H) s,1H) and 8.17 (br s, 1H)

468.02 468.03

9, 16.70 9, 16.04

0.05 g,

Example 288: 2.6-Difluoro-3-r4'-(4-methoxv-phenvl)-r4,5'1bithiazolvl-2'- ylmethoxy]-benzamide

To a solution of 3-[5-bromo-4-(4-methoxy-phenyl)-thiazol-2-ylmethoxy]-2,6-difluoro- benzamide (0.20 g, 0.043 mmol) in 5 ml of anhydrous DMF was added A- tributylstannyl thiazole (0.16 g , 0.43 mmol) and degassed the reaction mixture for 10 minutes. Tetrakis(triphenylphosphine) palladium (0) (0.05 g, 0.043 mmol) was then added and the reaction mixture was heated at 120⁰C for 12 h under the nitrogen atmosphere. Then reaction mixture was cooled to room temperature added water (25 mL) and extracted the compound with ethyl acetate (3 x 50 mL). The combined organic layers were dried over anhydrous Na₂SO₄, and evaporated to dryness under reduced pressure. The compound was purified by column chromatography on silica (230-400 M) using ethyl acetate/ Hexane (40:60) as the eluent to provide the title compound as white solid (0.072g, 36%). ¹H NMR (DMSOd₆, 400 MHz): δ 3.80 (s, 3H), 5.56 (S, 2H), 7.01 (d, J= 8.80 Hz, 2H), 7.13 (m, 1 H), 7.41-7.50 (m, 4H), 7.90 (br s, 1H), 8.18 (br s, 1H) and 9.18 (s, 1H). MS ES+ (460.32), HPLC (method II) Rt = 16.37 min. Example 289: 3-[5-Cvano-4-(4-methoxv-phenyl)-thiazol-2-vlmethoxv1-2.β- difluoro-benzamide

To a solution of 3-[5-bromo-4-(4-methoxy-phenyl)-thiazol-2-yImethoxy]-2,6-difluoro- benzamide (0.20 g, 0.43 mmol) in pyridine (4.0 ml_) was added CuCN (0.19 g, 2.19 mmol). The reaction mixture was heated to 15O⁰C in microwave for 2 h. After the completion of the reaction, pH was adjusted to 3-4 with 1 N HCI solution and extracted with ethyl acetate (3 x 50 ml_). The combined organics was washed with water, brine, dried (Na₂SO₄), filtered and concentrated. The crude residue was purified over silica gel (60-120 M, 45% EtOAc-Hexane) to get the desired product (0.02 g, 11%) as a brown solid. ¹H NMR (DMSOd₆, 400 MHz): δ 3.79 (s, 3H), 5.67 (s, 2H), 7.16 (m, 3H), 7.42 (m, 1 H), 7.88 (br s, 1H), 8.03 (d, J= 8.80 Hz, 2H) and 8.19 (br s, 1 H). MS ES+ (402.07), HPLC (method I) Rt = 16.60 min.

Scheme 32: (a) Zn/ Acetic acid; (b) BBr₃/DCM

Ref: Scheme-3

2,6-Difluoro-3-[4-(4-methoxy-phenyl)-thiazol-2-ylmethoxy]-benzamide

To a solution of 3-[5-bromo-4-(4-methoxy-phenyl)-thiazol-2-ylmethoxy]-2,6-difluoro- benzamide (2.0 g, 4.37 mmol) in the 50 ml of acetic acid was added Zn dust (2.0 g). The reaction mixture was heated at 120⁰C for 1 h. After the completion of the reaction mixture (TLC monitoring), water (100 mL) was added and pH was adjusted to 8-9 with NaOH solution and extracted with ethyl acetate (3 x 150 mL). The combined organics was washed with water, brine, dried (Na₂SO₄), filtered and concentrated to get the desired product (0.8 g, 50%) as a white solid.

Example 290: 2,6-Difluoro-3-r4-(4-hvdroxv-phenyl)-thiazol-2-ylmethoxv]- benzamide

A solution of 2,6-Difluoro-3-[4-(4-methoxy-phenyl)-thiazol-2-ylmethoxy]-benzamide (0.20 g, 0.53 mmol) in 15 ml of DCM was cooled to -78° C followed by addition of BBr₃ (0.2ml, 2.14 mmol). The reaction mixture was stirred at 25⁰C for 2 h. After the completion of the reaction mixture (TLC monitoring), solution of NaHCO₃ (20 mL) was added at O⁰C and extracted with ethyl acetate (3 x 50 mL). The combined organics was washed with water, brine, dried (Na₂SO₄), filtered and concentrated, the residue was purified by column chromatography on silica (60-120 M) using ethyl acetate/hexane (50:50) as the eluent to provide the title compound as light yellow solid (0.06 g, 31%). ¹H NMR (DMSO-d₆, 400 MHz): δ 5.55 (s, 2H), 6.83 (d, J= 8.40 Hz, 2H), 7.13 (m, 1 H), 7.40 (m, 1 H), 7.78 (d, J= 8.80 Hz, 2H), 7.88 (br s, 1H), 7.91 (s, 1H), 8.17 (br s, 1 H) and 9.64 (br s, 1 H). MS ES+(363.25), HPLC (method I) Rt = 14.57 min.

Scheme 33: (a) Thioacetamide; (b) NBS; (c) 2,6-difluoro-3-hydroxy benzamide, K₂CO₃, DMF.

4-(2-Methyl-thiazol-4-yl)-benzonitrile

The compound was prepared following the general method as described in the preparation of 4-(4-Methoxy-phenyl)-2-methyl-thiazole (Scheme 31).

4-(5-Bromo-2-bromomethyl-thiazol-4-yl)-benzonitrile

The compound was prepared following the general method as described in the preparation of 5-Bromo-2-bromomethyl-4-(4-methoxy-phenyl)-thiazole (Scheme 31).

Example 291 : 3-[5-Bromo-4-(4-cyano-phenyl)-thiazol-2-ylmethoxy]-2,6-dif luoro- benzamide

To a solution of 4-(5-Bromo-2-bromomethyl-thiazol-4-yl)-benzonitrile (0.43 g, 1.20 mmol) in 5 ml of anhydrous DMF was added 2,6-difluoro-3-hydroxy-benzamide (0.20 g, 1.20 mmol) and potassium carbonate (0.58 g, 4.20 mmol). The reaction mixture was stirred at 25°C for 24 h under nitrogen atmosphere. The reaction mixture was evaporated to dryness under reduced pressure and the residue was purified by column chromatography on silica (60-120 M) using ethyl acetate/hexane (30:70) as the eluent to provide the title compound as a white solid (0.35 g, 66%). ¹H NMR (DMSO-d₆, 400 MHz): δ 5.57 (s, 2H), 7.13 (m, 1H), 7.44 (m, 1H), 7.89 (br s, 1H), 8.0 (d, J= 8.40 Hz, 2H), 8.10 (d, J= 8.40 Hz, 2H) and 8.17 (br s, 1H). MS ES+(450.09), HPLC (method I) Rt = 16.127min.

Scheme 34: (a) NH₂OH-HCI, NaOH, EtOH; (b) Bromoacetyl bromide, (c) K₂CO₃, DMF.

Trifluoromethoxy phenyl -N-hydroxy-benzamide

To a solution of 4-Trifluoromethoxybenzonitrile (1.0 g, 5.0 mmol) in EtOH (20 mL) was added hydroxylamine hydrochloride (0.365 g, 5.0 mmol) and NaOH (0.212 g, 5.0 mmol). The resulting reaction mixture was refluxed for 15 h. After the completion of the reaction (TLC monitoring), the mixture was concentrated, added EtOH and filtered. The filtrate was evaporated in vacuo and used as such for the next step (crude yield 12.0 g, 66%).

5-BromomethyI-3-(Tri Fluoro Methoxy phenyl)-[1,2,4]oxadiazole

Bromoacetyl bromide (2.0 mL, 23.12 mmol) was added to trifluoromethoxy-N- hydroxy-benzamide (0.40 g, 5.86 mmol) and K₂CO₃ (0.87 g, 6.0 mmol). The reaction mixture was heated at 100⁰C for 15 min. After the completion of the reaction mixture (TLC monitoring), water (100 mL) was added and extracted with ethyl acetate (3 x 50 mL). The combined organics was washed with water, brine, dried (Na₂SO₄), filtered and concentrated. The crude residue was purified over silica gel (60-120 M, 3% EtOAc-Hexane) to get the desired product (0.25 g, 43%) as a white solid.

Example 292: 3-[3-(4-Trifluoromethoxy-phenyl)-[1 ,2,4]oxadiazol-5-ylmethoxy]-2- fluoro-benzamide

To a solution of 5-Bromomethyl-3-(Trifluoromethoxy phenyl)-[1 ,2,4]oxadiazole (0.24 g, 1.0 mmol) in 2.5 ml of anhydrous DMF was added 2,6-difluoro-3-hydroxy benzamide (0.18 g, 1.0 mmol) and potassium carbonate (0.516 g, 3.7 mmol). The reaction mixture was stirred at 25°C for 24 h under nitrogen atmosphere. The reaction mixture was evaporated to dryness under reduced pressure and the residue was purified by column chromatography on silica (60-120 M) using ethyl acetate/hexane (50:50) as the eluent to provide the title compound as white solid (0.090 g, 20%). ¹H NMR (DMSO-d₆, 400 MHz): δ 5.71 (s, 2H), 7.15 (t, J= 7.60 Hz, 1H), 7.40 (m, 1H), 7.60 (d, J= 8.0 Hz, 2H), 7.91 (br s, 1 H), 8.15 (d, J= 8.40 Hz, 2H) and 8.18 (br s, 1H). MS ES+(416.28), HPLC (method I) Rt = 16.79 min.

X=CI, Example 294 X=OMe, Example 293 X= Me, Example 295

4-Chloromethyl-2-(4-methoxy-phenyl)-oxazole (General method)

To a solution of 1 ,3 dichloroacetone (0.504 g, 3.90 mmol) in toluene (5 ml) was added 4-methoxy benzamide (0.30 g, 1.90 mmol). The reaction mixture was heated at 12O⁰C for 1 h. After the completion of the reaction mixture (TLC monitoring), the reaction mixture was evaporated to dryness under reduced pressure and the residue was purified over silica gel (230-400 M, 15% EtOAc-Hexane) to get the desired product (0.37 g, 83%). Example 293: 2,6-Pifluoro-3-[2-(4-methoxy-phenyl)-oxazol-4-ylmethoxv1- benzamide

To a solution of 4-Chloromethyl-2-(4-methoxy-phenyl)-oxazole (0.100 g, 0.4mmol) in 2 ml of anhydrous DMF was added 2,6-Difluoro-3-hydroxy-benzamide (0.077 g, 0.40 mmol) and potassium carbonate (0.216 g, 1.50 mmol). The reaction mixture was stirred at 25°C for 24 h under nitrogen atmosphere. The reaction mixture was evaporated to dryness under reduced pressure and the residue was purified by column chromatography on silica (60-120 M) using ethyl acetate/hexane (50:50) as the eluent to provide the title compound as white solid (0.044 g, 27%). ¹H NMR (DMSOd₆, 400 MHz): δ 4.01 (s, 3H), 5.12 (s, 2H), 7.10 (m, 3H), 7.40 (m, 1 H), 7.85 (br s, 1H), 7.93 (d, J= 8.80 Hz, 2H), 8.13 (br s, 1 H) and 8.25 (s, 1 H). MS ES+(361.16), HPLC (method I) Rt = 15.47 min.

Example 294: 3-[2-(4-Chloro-phenyl)-oxazol-4-ylmethoxy]-2,6-difluoro- benzamide

To a solution of 4-ChIoromethyl-2-(4-chloro-phenyl)-oxazole (0.20 g, 0.87 mmol) in 2 ml of anhydrous DMF was added 2,6-Difluoro-3-hydroxy-benzamide (0.15 g, 0.78 mmol) and potassium carbonate (0.363 g, 2.60 mmol). The reaction mixture was stirred at 25°C for 24 h under nitrogen atmosphere. The reaction mixture was evaporated to dryness under reduced pressure and the residue was purified by column chromatography on silica (60-120 M) using ethyl acetate/hexane (50:50) as the eluent to provide the title compound as white solid (0.10 g, 31%). ¹H NMR (DMSOd₆, 400 MHz): δ 5.14 9s, 2H), 7.12 (t, J= 9.20 Hz, 1H), 7.40 (m, 1H), 7.63 (d, J= 8.40, 2H), 7.85 (br s, 1 H), 8.0 (d, J= 8.40 Hz, 2H), 8.13 (br s, 1 H) and 8.36 (s, 1 H). MS ES+(365.13), HPLC (method I) Rt = 16.36 min.

Example 295: 2,6-Difluoro-3-(2-p-tolyl-oxazol-4-ylmethoxy)-benzamide

To a solution of 4-Chloromethyl-2-p-tolyl-oxazole (0.10 g, 0.50 mmol) in 2 ml of anhydrous DMF was added 2,6-Difluoro-3-hydroxy-benzamide (0.08 g, 0.50 mmol) and potassium carbonate (0.233g, 1.50 mmol). The reaction mixture was stirred at 25°C for 24 h under nitrogen atmosphere. The reaction mixture was evaporated to dryness under reduced pressure and the residue was purified by column chromatography on silica (60-120 M) using ethyl acetate/hexane (50:50) as the eluent to provide the title compound as white solid (0.03 g, 18%). ¹H NMR (DMSO-d₆, 400 MHz): δ 2.37 (s, 3H), 5.13 (s, 2H), 7.11 (m, 1 H), 7.36 (d, J= 8.0 Hz, 2H), 7.41 (m, 1 H), 7.88 (m, 3H), 8.12 (br s, 1 H) and 8.29 (s, 1 H). MS ES+(345.24), HPLC (method I) Rt = 16.07 min.

Scheme 36:

1. X= OMe, Example 296 2. X= Cl, Example 297 2-(4-Methoxy-phenyl)-4,5-dimethyl-oxazole (General method)

A mixture of 3-Chloro-2-butanone (2.1g, 10.0 mmol) and 4-methoxybenzamide (0.30 g, 1.0 mmol) was heated at 115⁰C for 15 h under nitrogen atmosphere. After the completion of the reaction mixture (TLC monitoring), the reaction mixture was evaporated to dryness under reduced pressure and the residue was purified over silica gel (230-400 M, 20% EtOAc-Hexane) to get the desired product (0.17 g, 42%) as a white solid. The corresponding chloro derivative was also prepared by the same general method.

4-Bromomethyl-2-(4-methoxy-phenyl)-5-methyl-oxazole

To the solution of 4-Bromomethyl-2-(4-methoxy-phenyl)-5-methyl-oxazole (0.17 g , 0.80 mmol) in acetonitrile (4.0 mL) was added NBS (7.43 g, 41.74 mmol). The reaction mixture was stirred at 25⁰C for 1 h under nitrogen atmosphere. After the completion of the reaction mixture (TLC monitoring), the reaction mixture was cooled to O⁰C and 2 ml of water was added. The resulting precipitate was filtered and dried to give the desired product (0.11 g, 46%). The corresponding chloro derivative was also prepared by the same general method.

Example 296: 2,6-Difluoro-3-r2-(4-methoxv-phenvl)-5-methvl-oxazol-4- ylmethoxy]-benzamide

To a solution of 4-Bromomethyl-2-(4-methoxy-phenyl)-5-methyl-oxazole (0.10 g, 0.35 mmol) in 2 ml of anhydrous DMF was added 2,6-Difluoro-3-hydroxy-benzamide (0.061 g, 0.35 mmol) and potassium carbonate (0.171 g, 1.05 mmol). The reaction mixture was stirred at 25°C for 24 h under nitrogen atmosphere. The reaction mixture was evaporated to dryness under reduced pressure and the residue was purified by column chromatography on silica (60-120 M) using ethyl acetate/hexane (50:50) as the eluent to provide the title compound as white solid (0.117 g, 87%). ¹H NMR (DMSO-de, 400 MHz): δ 2.42 (s, 3H), 3.82 (s, 3H), 5.06 (s, 2H), 7.10 (m, 3H), 7.37 (m, 1 H), 7.86 (m, 3H) and 8.13 (br s, 1 H). MS ES+(375.12), HPLC (method I) Rt = 15.78 min.

Example 297: 3-[2-(4-Chloro-phenyl)-5-methyl-oxazol-4-ylmethoxy]-2,6-difluoro- benzamide

To a solution of 4-Bromomethyl-2-(4-chIoro-phenyl)-5-methyl-oxazole (0.12 g, 0.42 mmol) in 2 ml of anhydrous DMF was added 2,6-Difluoro-3-hydroxy-benzamide (0.072 g, 0.42 mmol) and potassium carbonate (0.203 g, 1.20 mmol). The reaction mixture was stirred at 25°C for 24 h under nitrogen atmosphere. The reaction mixture was evaporated to dryness under reduced pressure and the residue was purified by column chromatography on silica (60-120 M) using ethyl acetate/hexane (50:50) as the eluent to provide the title compound as white solid (0.01 g, 6%). ¹H NMR (DMSO- d₆, 400 MHz): δ 2.49 (s, 3H), 5.09 (s, 2H), 7.11 (m, 1 H), 7.38 (m, 1 H), 7.60 (d, J= 8.40 Hz, 2H), 7.85 (br s, 1H), 7.95 (d, J= 8.40 Hz, 2H) and 8.13 (br s, 1H). MS ES+(379.25), HPLC (method I) Rt = 16.71 min.

Scheme 37: (a) PBr₃; (b) SnCI₂.2H₂O; (c) 2-Benzoyloxy acetyl chloride; (d) Lawesson's reagent; (e) BBr₃; (f) 2,6-difluoro-3-hydroxy benzamide, K₂CO₃, DMF; (g) Suzuki or Stannyl conditions.

N^ .OH N_^ .Br N^ .Br N_^ ^Br OBn

.OBn

Br NO₂ Br NO₂ Br NH₂ Br NH Br

2,5-Dibromo-3-nitro-pyridine

To a solution of 5-Bromo-3-nitro-pyridin-2-ol (10.0 g, 45.66 mmol) in 70 ml of toluene and 7 ml of DMF was added PBr₃ (6.60 ml, 68.49 mmol) and the reaction mixture was heated at 12O⁰C for 20 min under nitrogen atmosphere. After the completion of the reaction mixture (TLC monitoring), water (100 mL) was added and extracted with ethyl acetate (3 x 200 mL). The combined organics was washed with water, brine, dried (Na₂SO₄), filtered and concentrated to get the desired product (10.30 g, 80.03%).

2,5-Dibromo-pyridin-3-ylamine

To the solution of 2,5-Dibromo-3-nitro-pyridine (10.30 g, 35.47 mmol) in the 100 ml of ethanol was added SnCI₂ (24.0 g, 106.42 mmol) slowly. The reaction mixture was heated at 80⁰C for 2 h. After the completion of the reaction mixture (TLC monitoring), the reaction mixture was evaporated to dryness under reduced pressure. Water (250 ml_) was added, white solid separated out, then, basified the reaction mixture with NaOH Solution. To this added the 250 ml of ethyl acetate. Filtered it and washed the residue with ethyl acetate, layers are separated, dried (Na₂SO₄), filtered, concentrated to give the desired product (6.2Og, 67.39%).

2-Benzyloxy-N-(2,5-dibromo-pyridin-3-yl)-acetamide

To the solution of 2,5-Dibromo-pyridin-3-ylamine (8.6 g, 34.12 mmol) in 50 ml of DCM was added triethylamine (5.3 ml, 37.53 mmol). Cooled the reaction mixture to

O⁰C. To this added the solution of 2-benzyloxy acetyl chloride (7.45 g, 40.95 mmol) in

35 ml of DCM. The reaction mixture was stirred at 25⁰C for 12 hr. After the completion of the reaction mixture (TLC monitoring), the reaction mixture was evaporated to dryness under reduced pressure. The residue was purified by column chromatography on silica (60-120 M) using ethyl acetate/hexane (10:90) as the eluent to provide the title compound (3.2 g, 24.17%).

2-Benzyloxymethyl-5-bromo-thiazolo[5,4-b]pyridine

To the solution of 2-Benzyloxy-N-(2,5~dibromo-pyridin-3-yl)-acetamide (2.5 g, 6.248 mmol) in 30 ml of toluene was added Lawesson's reagent (1.51g, 3.74 mmol). The reaction mixture was heated at 12O⁰C for 2 h. After the completion of the reaction mixture (TLC monitoring), the reaction mixture was evaporated to dryness under reduced pressure. The residue was purified by column chromatography on silica (60- 120 M) using ethyl acetate/hexane (5:95) as the eluent to provide the title compound (1.6O g, 76.5%). 5-bromo-2-bromomethyl-thiazolo[5,4-b]pyridine

A solution of 2-Benzyloxymethyl-5-bromo-thiazolo[5,4-b]pyridine (1.60 g, 4.77 mmol) DCM (15 ml.) was cooled to -78° C followed by addition of BBr₃ (2.27 ml, 23.86 mmol). The reaction mixture was stirred at 25⁰C for 2 h. After the completion of the reaction mixture (TLC monitoring), solution of NaHCO₃ (20 ml_) was added at 0° C and extracted with ethyl acetate (3 x 50 ml_). The combined organics was washed with water, brine, dried (Na₂SO₄), filtered and concentrated to get the desired product (2.0 g, Crude yield).

Example 298: 3-(5-bromo-thiazolor5,4-b1pvridin-2-ylmethoxv)-2,6-difluoro- benzamide

To a solution of 5-bromo-2-bromomethyl-thiazolo [5,4-b] pyridine (2.0 g, 6.493mmol) in 10 ml of anhydrous DMF was added 2,6-Difluoro-3-hydroxy-benzamide (1.01 g,

5.84 mmol) and potassium carbonate (3.09 g, 22.72 mmol). The reaction mixture was stirred at 25°C for 24 h under nitrogen atmosphere. The reaction mixture was evaporated to dryness under reduced pressure and the residue was purified by column chromatography on silica (60-120 M) using ethyl acetate/hexane (50:50) as the eluent to provide the title compound (1.80 g, 69%). ¹H NMR (DMSO-d₆, 400

MHz): δ 5.72 (s, 2H), 7.12 (t, J= 7.60 Hz, 1 H), 7.39 (m, 1 H), 7.90 (br s, 1 H), 8.18 (br s, 1H) and 8.80 (m, 2H). MS ES+(402.08), HPLC (method I) Rt = 15.50 min.

3-(5-Allyl-thiazolo[5,4-b]pyridin-2-ylmethoxy)-2,6-difluoro-benzamide

To a solution of 3-(5-bromo-thiazolo[5,4-b]pyridin-2-ylmethoxy)-2,6-difluoro- benzamide (0.15 g, 0.37 mmol) in 5 ml of anhydrous DMF was added allyl tributyltin (0.26 ml , 0.86 mmol) and degassed the reaction mixture for the 10 minutes. Tetrakis(triphenylphosphine) palladium (0) (0.007 g, 0.0056 mmol) was then added and the reaction mixture was heated at 120⁰C for 1h under the nitrogen atmosphere. Then reaction mixture was cooled to room temperature added water (25 ml.) and extracted the compound with ethyl acetate. The combined organic layers were dried over anhydrous Na₂SO₄, and evaporated to dryness under reduced pressure. The compound was purified by column chromatography on silica (100-200 M) using ethyl acetate/ Hexane (60:40) as the eluent to provide the title compound (0.10 g, 75%).

Example 299: 2.β-Difluoro-3-f5-prøpvl-thiazolor5.4-b1Pvridin-2-vlmethoxv)- benzamide

To a solution of 3-(5-Allyl-thiazolo[5,4-b]pyridin-2-ylmethoxy)-2,6-difluoro-benzamide (0.018 g, 0.049 mmol) in 5 ml of anhydrous methanol was added Pd-C (10%, 5 mg) and the reaction mixture was stirred at 25°C for 12 h under hydrogen atmosphere. The reaction mixture was filtered over the bed of celite and the filtrate was evaporated to dryness under reduced pressure to give the title compound as white solid (0.0078 g, 43%). ¹H NMR (DMSO-d₆, 400 MHz); δ 0.91 (m, 3H), 1.65 (m, 2H), 2.74 (m, 2H), 5.69 (s, 2H), 7.12 (m, 1H), 7.39 (m, 1 H), 7.90 (br s, 1 H), 8.18 (br s, 1H), 8.27 (br s, 1 H) and 8.52 (br s, 1 H). MS ES+ (364.11), HPLC (method I) Rt = 15.85 min.

Example 300: 2,6-Dif luoro-3-[5-(1 -methyl-1 H-imidazol-2-yl)-thiazolo[5,4- b]pyridin-2-ylmethoxy]-benzamide

To a solution of 3-(5-bromo-thiazolo[5,4-b]pyridin-2-ylmethoxy)-2,6-difluoro- benzamide (0.10 g, 0.24 mmol) in 5 ml of anhydrous DMF was added 1-methyl-2- tributylstannanyl-1 H-imidazole (0.120 g , 0.32 mmol) and degassed the reaction mixture for the 10 minutes. Tetrakis(triphenylphosphine) palladium (0) (0.004 g, 0.0037 mmol) was then added and the reaction mixture was heated at 120⁰C for 12 h under the nitrogen atmosphere. The reaction mixture was then cooled to room temperature, added water (25 ml_) and extracted the compound with ethyl acetate. The combined organic layers were dried over anhydrous Na₂SO₄, and evaporated to dryness under reduced pressure. The compound was purified by column chromatography on silica (230-400 M) using ethyl acetate/ hexane (40:60) as the eluent to provide the title compound as brick red solid (0.020 g, 20%). ¹H NMR (DMSOd₆, 400 MHz): δ 3.14 (s, 3H), 5.67 (s, 2H), 7.07 (m, 1H), 7.28-7.37 (m, 2H), 7.87 (m, 2H), 8.28 (s, 1H), 8.53 (s, 1H) and 8.75 (br s, 1 H). MS ES+(402.22), HPLC (method I) Rt = 12.05 min.

Example 301: 2,6-Difluoro-3-[5-(1 -methyl-1 H-pyrrol-2-yl)-thϊazolo[5,4-b]pyridin- 2-ylmethoxy]-benzamide

To a solution of 3-(5-bromo-thiazolo[5,4-b]pyridin-2-ylmethoxy)-2,6-difluoro- benzamide (0.10 g, 0.24 mmol) in 5 ml of anhydrous DMF was added 1-methyl-2- tributylstannanyl-1 H-pyrrole (0.120 g , 0.32 mmol) and degassed the reaction mixture for the 10 minutes. Tetrakis(triphenylphosphine) palladium (0) (0.004 g, 0.0037 mmol) was then added and the reaction mixture was heated at 120⁰C for 12 h under the nitrogen atmosphere. The reaction mixture was then cooled to room temperature, added water (25 ml.) and extracted the compound with ethyl acetate. The combined organic layers were dried over anhydrous Na₂SO₄, and evaporated to dryness under reduced pressure. The compound was purified by column chromatography on silica (230-400 M) using ethyl acetate/ Hexane (40:60) as the eluent to provide the title compound as yellow solid (0.032 g, 32%). ¹H NMR (DMSO-d₆, 400 MHz): δ 3.73 (s, 3H), 5.72 (s, 2H), 6.13 (br s, 1 H), 6.40 (br s, 1 H), 6.97 (s, 1 H), 7.12 (m, 1 H), 7.42 (m, 1 H), 7.90 (br s, 1 H), 8.18 (br s, 1 H), 8.48 (s, 1 H) and 8.75 (s, 1 H). MS ES+(401.26), HPLC (method I) Rt = 15.61 min. Example 302: 2,6-Difluoro-3-(5-phenyl-thiazolo[5,4-b]pyridin-2-ylmethoxy)- benzamide

To a solution of 3-(5-bromo-thiazolo[5,4-b]pyridin-2-ylmethoxy)-2,6-difluoro- benzamide (0.20 g, 0.49 mmol) in 4 ml of DMF and water (2.0 ml) was added phenyl boronic acid (0.12 g, 0.99 mmol) and potassium phosphate (0.13 g, 0.59 mmol). The reaction mixture was degassed for 10 minutes followed by addition of dichlorobis(tri phenyl phosphine) palladium (II) (0.070 g, 0.099 mmol). The reaction mixture was heated at 120⁰C for 2h under the nitrogen atmosphere. After the completion of the reaction mixture (TLC monitoring), water (25 ml.) was added and extracted with ethyl acetate (3 x 50 ml_). The combined organics was washed with water, brine, dried (Na₂SO₄), filtered and concentrated. The crude residue was purified over silica gel (100-200 M, 60% EtOAc-Hexane) to get the desired product (0.080 g, 41%) as a beige solid. ¹H NMR (DMSO-d₆, 400 MHz): δ 5.74 (s, 2H), 7.10 (m, 1H), 7.41-7.56 (m, 4H), 7.85 (m, 3H), 8.19 (m, 1 H), 8.71 (br s, 1 H) and 8.98 (br s, 1H). MS ES+ (398.09), HPLC (method I) Rt = 16.07 min.

Scheme 38:

X=Me₁Y=H; Example 303

X=OH, Y=H; Example 304

X=F, Y=H; Example 305

X=OCF3, Y=H; Example

307

X=CI, Y= H; Example 306

X=H, Y=OH; Example 308

lyl-thiazole (Representative example)

To a solution of 1 ,3 dichloroacetone (0.84 g, 6.62 mmol) in toluene (5 ml) was added 4-methylthiobenzamide (0.50 g, 3.31 mmol) and the reaction mixture was heated at 120⁰C for 1 h. After the completion of the reaction mixture (TLC monitoring), the reaction mixture was evaporated to dryness under reduced pressure and the residue was purified over silica gel (230-400 M, 15% EtOAc-Hexane) to get the desired product (0.49 g, 67%). The other derivatives were also prepared by the same general method.

3-(4-Chloromethyl-thiazol-2-yl)-phenol

To a solution of 1 ,3 dichloroacetone (0.42 g, 3.26 mmol) in toluene (5 mL) was added 3-hydroxythiobenzamide (0.25 g, 1.63 mmol) and the reaction mixture was heated at 120⁰C for 1 h. After completion of the reaction mixture (TLC monitoring), the reaction mixture was evaporated to dryness, added water and extracted with EtOAc (x 3). The combined organics was washed with water, brine, dried (Na₂SO₄), filtered and concentrated. The crude residue was purified over silica gel (230-400 M, 10% EtOAc-Hexane) to get the desired product (0.14 g, 38%).

Scheme 39: (a) 2-Benzyloxy acetamide, DMF; (b) BBr₃, DCM; (c) 2,6-Difluoro-3- hydroxybenzamide, K₂CO₃, DMF.

X=H, Example 310 X= Cl, Example 309

2-Benzyloxymethyl-4-(4-chloro-phenyl)-oxazole (Representative Procedure)

To a solution of 2-Benzyloxy-acetamide (1.40 g, 8.56 mmol) in 4 ml of DMF was added 2-Bromo-1-(4-chloro-phenyl)-ethanone (2.0 g, 8.56 mmol) and the reaction mixture was heated at 13O⁰C for 6 h under nitrogen atmosphere. After the completion of the reaction mixture (TLC monitoring), water (25 mL) was added and extracted with ethyl acetate (3 x 50 mL). The combined organics was washed with water, brine, dried (Na₂SO₄), filtered and concentrated. The crude residue was purified over silica gel (230-400 M, 10% EtOAc-Hexane) to get the desired product (1.1 g, 44%).

2-Bromomethyl-4-(4-chIoro-phenyl)-oxazole (Representative procedure)

A solution of 2-Benzyloxymethyl-4-(4-chloro-phenyl)-oxazole (1.10 g, 3.6 mmol) in 10 ml of DCM was cooled to -78° C followed by addition of BBr₃ (1.76 ml, 18.0 mmol). The reaction mixture was stirred at 25⁰C for 2 h. After the completion of the reaction mixture (TLC monitoring), solution of NaHCO₃ (20 mL) was added at O⁰C and extracted with ethyl acetate (3 x 50 mL). The combined organics was washed with water, brine, dried (Na₂SO₄), filtered and concentrated to get the desired product (0.5g, 49%, crude).

Examples 303-310 (Table Q)

The compounds of Examples 303-310 were synthesised according to the following general procedure: To a solution of reactant (A) in 2 ml of anhydrous DMF was added 2,6-Difluoro-3-hydroxy-benzamide (B) and potassium carbonate (C). The reaction mixture was stirred at 25 ⁰C for 24 h under nitrogen atmosphere. The reaction mixture was evaporated to dryness under reduced pressure and the residue was purified by column chromatography on silica (60-120 M) using ethyl acetate/hexane (50:50) as the eluent to provide the product compound.

Table Q

0.405 g,

1 H), 7.32- 7.88 (s, 1H)

Scheme 40: (a) Acetamide; (b) NBS, AIBN, CCI₄; (c) 2,6-difluoro-3-hydroxy benzamide, K₂CO₃, DMF; (d) Zn/Acetic aciid; (e) BBr₃, DCM.

4-(4-Methoxy-phenyl)-2-methyl-oxazole

Prepared as per the method mentioned in Scheme 31.

5-Bromo-2-bromomethyl-4-(4-methoxy-phenyl)-oxazole

Prepared as per the method mentioned in Scheme 31.

3-[5-Bromo-4-(4-methoxy-phenyl)-oxazol-2-ylmethoxy]-2,6-difluoro-benzamide

Prepared as per the method mentioned in Scheme 31.

Example 311 : 2,6-Difluoro-3-r4-(4-methoxy-phenyl)-oxazol-2-vlmethoxvT- benzamide

To a solution of 3-[5-Bromo-4-(4-methoxy-phenyl)-oxazol-2-ylmethoxy]-2,6-difluoro- benzamide (0.06g, 0.13 mmol) in the 5 ml of acetic acid was added 50 mg of Zn dust. Reaction mixture was heated at 120⁰C for 1 h. After the completion of the reaction mixture (TLC monitoring), water (25 mL) was added and pH was adjusted to 8-9 with NaOH solution and extracted with ethyl acetate (3 x 50 mL). The combined organics was washed with water, brine, dried (Na₂SO₄), filtered and concentrated to get the desired product (0.02 g, 40%) as a white solid. ¹H NMR (DMSO-d₆, 400 MHz): δ 3.77 (s, 3H), 5.36 (s, 2H), 6.99 (d, J= 8.40 Hz, 2H), 7.12 (m,1H), 7.37 (m, 1H), 7.71 (d, J= 8.40 Hz, 2H), 7.87 (br s, 1 H), 8.15 (br s,1 H) and 8.56 (s, 1 H). MS ES+ (361.24), HPLC (method I) Rt = 15.41 min.

Example 312: 2,6-Difluoro-3-r4-(4-hvdroxv-phenyl)-oxazol-2-vlmethoxv1- benzamide

A solution of 2,6-Difluoro-3-[4-(4-methoxy-phenyl)-oxazol-2-ylmethoxy]-benzamide (0.20 g, 0.55 mmol) in 10 ml of DCM was cooled to -78° C followed by addition of BBr₃ (0.10 ml, 2.20 mmol). The reaction mixture was stirred at 25⁰C for 2 h. After the completion of the reaction mixture (TLC monitoring), solution of NaHCO₃ (20 mL) was added at 0° C and extracted with ethyl acetate (3 x 50 mL). The combined organics was washed with water, brine, dried (Na₂SO₄), filtered and concentrated. The residue was purified by column chromatography on silica (60-120 M) using ethyl acetate/hexane (50:50) as the eluent to provide the title compound as white solid (0.012 g, 6%). ¹H NMR (DMSO-d₆, 400 MHz): δ 5.35 (s, 2H), 6.82 (d, J= 8.40 Hz, 2H), 7.14 (m, 1 H), 7.38 (m, 1 H), 7.58 (d, J= 8.40 Hz, 2H), 7.87 (br s, 1H), 8.15 (br s, 1H), 8.47 (S, 1H) and 9.63 (s, 1H). MS ES+(347.22), HPLC (method I) Rt = 14.00 min.

Scheme 41 : (a) Thioacetamide; (b) NBS, AIBN, CCI₄; (c) CuCN, Pyridine; (d) MeOH, dry HCI; (e) NBS, AIBN, CCI₄; (f) 2,6-difluoro-3-hydroxy benzamide, K₂CO₃, DMF.

4-(4-Methoxy-phenyl)-2-methyl-thiazoIe

A mixture of thioacetamide (16.0 g, 213 mmol) and 2-Bromo-1-(4-methoxy-phenyl)- ethanone (4.0 g, 17.5 mmol) was heated at 14O⁰C for 24 h under nitrogen atmosphere. After the completion of the reaction mixture (TLC monitoring), water (25 mL) was added and extracted with ethyl acetate (3 x 50 ml_). The combined organics was washed with water, brine, dried (Na₂SO₄), filtered and concentrated. The crude residue was purified over silica gel (230-400 M, 1% EtOAc-Hexane) to get the desired product (2.5 g, 69%).

5-Bromo-4-(4-methoxy-phenyl)-2-methyl-thiazole

To the solution of 5-Bromo-2-bromomethyl-4-(4-methoxy-phenyl)-thiazole (5.0 g, 24.3 mmol) in the 20 ml of CCI₄ was added NBS (4.32 g, 24.3 mmol) and AIBN ( 0.4 g, 2.43 mmol). The reaction mixture was heated at 100⁰C for 2 h under nitrogen atmosphere. After the completion of the reaction mixture (TLC monitoring), the reaction mixture was evaporated to dryness under reduced pressure and the residue was purified by column chromatography on silica (230-400 M) using 1% ethyl acetate/hexane eluent to give the desired product (4.0 g, 58%).

4-(4-Methoxy-phenyl)-2-methyl-thiazole-5-carbonitrile

To a solution of 5-Bromo-4-(4-methoxy-phenyl)-2-methyl-thiazole (2.0 g, 7.0 mmol) in 15 ml of pyridine was added CuCN (3.10 g, 35.2 mmol)and the reaction mixture was heated to 150⁰C in microwave for 2 h. After the completion of the reaction pH was adjusted to 3-4 with 1 N HCI solution and extracted with ethyl acetate (3 x 50 ml_). The combined organics was washed with water, brine, dried (Na₂SO₄), filtered and concentrated. The crude residue was purified over silica gel (60-120 M, 12% EtOAc- Hexane) to get the desired product (1.5 g, 92%) as a white solid.

4-(4-Methoxy-phenyl)-2-methyl-thiazole-5-carboxylic acid methyl ester

To a solution of 4-(4-Methoxy-phenyl)-2-methyl-thiazole-5-carbonitrile (0.50 g, 2.1 mmol) in 15 ml of methanol was passed dry HCI gas for 1 h at 0° C. The reaction mixture was stirred at 25⁰C for 24 h. After the completion of the reaction mixture (TLC monitoring), The reaction mixture was evaporated to dryness under reduced pressure. Water (50 ml) was added and pH was adjusted to 7-8 with NaHCO₃ solution and extracted with ethyl acetate (3 x 50 ml_). The combined organics was washed with water, brine, dried (Na₂SO₄), filtered and concentrated to get the desired product (0.25g, 44%) as a white solid.

2-Bromomethyl-4-(4-methoxy-phenyl)-thiazole-5-carboxylic acid methyl ester

To the solution of 4-(4-Methoxy-phenyl)-2-methyl-thiazole-5-carboxylic acid methyl ester (0.25 g, 0.94 mmol) in the 20 ml of CCI₄ was added NBS (0.16 g, 0.94 mmoi) and AIBN ( 0.015 g, 0.094 mmol). The reaction mixture was heated at 100⁰C for 2 h under nitrogen atmosphere. After the completion of the reaction mixture (TLC monitoring), the reaction mixture was evaporated to dryness under reduced pressure and the residue was purified by column chromatography on silica (230-400 M) using 10% ethyl acetate/hexane as a eluent to give the desired product (0.078 g, 24%).

Example 313: 2-(3-Carbamoyl-2,4-difluoro-phenoxvmethyl)-4-(4-methoxy- phenyl)-thiazole-5-carboxylic acid methyl ester

To a solution of 2-Bromomethyl-4-(4-methoxy-phenyl)-thiazole-5-carboxylic acid methyl ester (0.05 g, 0.14 mmol) in 2 ml of anhydrous DMF was added 2,6-Difluoro- 3-hydroxy-benzamide (0.025 g, 0.14 mmol) and potassium carbonate (0.07 g, 0.50 mmol). The reaction mixture was stirred at 25°C for 24 h under nitrogen atmosphere. The reaction mixture was evaporated to dryness under reduced pressure and the residue was purified by column chromatography on silica (60-120 M) using ethyl acetate/hexane (50:50) as the eluent to provide the title compound as white solid (0.025g, 40%). ¹H NMR (DMSO-d₆, 400 MHz): δ 3.76 (s, 3H), 3.81 (s, 3H), 5.60 (s, 2H), 7.01 (d, J= 8.40 Hz, 2H), 7.12 (m, 1 H), 7.41 (m, 1 H), 7.74 (d, J= 8.40 Hz, 2H), 7.90 (br s, 1 H) and 8.18 (br s, 1H). MS ES+(435.06), HPLC (method I) Rt = 15.86 min.

Scheme 42: (a) Triflic anhydride, pyridine, DCM; (b) 2,6-Difluoro-3-[5-(4,4,5,5- tetramethyl-[1 ,3,2]dioxaborolan-2-yl)-1 H-inden-2-ylmethoxy]-benzamide, Pd catalyst, Potassium phosphate; (c) H₂, Pd-C.

Trjfluoromethanesulfonic acid cyclohex-1-enyl ester

OTf To a solution of cyclohexanone (5.0 g, 51 mmol) in the 80 ml of DCM was added pyridine (4.48 ml, 56.0 mmol) and the resulting reaction mixture was cooled to -78⁰C. To the reaction mixture the solution of triflic anhydride (7.40 ml, 56.0 mmol) in 30 ml of DCM was added over the period of 1 h. Reaction mixture was stirred at 25° C for 24 h. After the completion of the reaction mixture (TLC monitoring), the reaction mixture was evaporated to dryness under reduced pressure. The residue was triturated with n-pentane and decanted the organic layer, dried (Na₂SO₄), filtered and concentrated to give the desired product (5.0 g, 42%).

Example 314: 3-(5-Cyclohex-1-enyl-1 H-inden-2-ylmethoxy)-2,6-difluoro- benzamide

To a solution of 2,6-Difluoro-3-[5-(4,4,5,5-tetramethyl-[1,3,2]dioxaborolan-2-yl)-1H- inden-2-ylmethoxy]-benzamide (0.10 g, 0.20 mmol) in 3 ml of anhydrous DMF and water (1.5 ml) was added trifluoromethanesulfonic acid cyclohex-1-enyl ester (0.15 g,

0.60 mmol) and potassium phosphate (0.057 g, 0.20 mmol). The reaction mixture was degassed for 10 minutes followed by addition of dichlorobis(triphenyl phosphine) palladium (II) (0.02 g, 0.03 mmol). The reaction mixture was heated at 8O⁰C for 1 h under the nitrogen atmosphere. After the completion of the reaction mixture (TLC monitoring), water (25 mL) was added and extracted with ethyl acetate (3 x 50 mL).

The combined organics was washed with water, brine, dried (Na₂SO₄), filtered and concentrated. The crude residue was purified over silica gel (230-400 M, 45%

EtOAc-Hexane) to get the desired product (0.017g, 19%) as a white solid. ¹H NMR (DMSO-d₆, 400 MHz): δ 1.63 (m, 4H), 2.20 (m, 2H), 2.45 (m, 2H), 5.67 (s, 2H), 6.29

(m, 1H), 7.12 (m, 1 H), 7.37 (m, 1 H), 7.57 (m, 2H), 7.90 (br s, 1 H), 8.03 (d, J= 8.40

Hz, 1H) and 8.18 (br s, 1H). MS ES+ (401.16), HPLC (method II) Rt = 14.13 min.

Example 315: 3-(5-Cyclohexyl-1 H-inden-2-ylmethoxy)-2,6-difluoro-benzamide

To a solution of 3-(5-Cyclohex-1-enyl-1H-inden-2-ylmethoxy)-2,6-difluoro-benzamide (0.01 g, 0.25 mmol) in 5 ml of anhydrous methanol was added Pd-C (10%, 100 mg). The reaction mixture was stirred at 25⁰C for 48 h under hydrogen atmosphere. The reaction mixture was filtered over the bed of celite and evaporated to dryness under reduced pressure to give the title compound as white solid (0.01 g, 10%). ¹H NMR (DMSO-de, 400 MHz); δ 1.40 (m, 6H), 1.72 (m, 4H), 2.63 (m, 1H), 5.66 (s, 2H), 7.09 (m, 1 H), 7.35 (m, 2H), 7.83 (br s, 1 H), 7.89 (d, J= 8.40 Hz,1 H), 7.99 (d, J= 8.40 Hz, 1 H) and 8.18 (br s, 1H). MS ES+ (403.33), HPLC (method II) Rt = 18.76 min.

Scheme 43: (a) Triflic anhydride, pyridine, DCM; (b) 2,6-Difluoro-3-[5-(4,4,5,5- tetramethyl-[1 ,3,2]dioxaborolan-2-yl)-1 H-inden-2-ylmethoxy]-benzamide, Pd catalyst, Potassium phosphate; (c) H₂, Pd-C.

Example 316 Example 317

Trifluoro-methanesulfonic acid cyclopent-1 -enyl ester

To a solution of cyclopentanone (5.0 g, 59 mmol) in the 80 ml of DCM was added pyridine (5.2 ml, 65.0 mmol) and the resulting reaction mixture was cooled to -78⁰C. To the reaction mixture the solution of triflic anhydride (9.2 ml, 65.0 mmol) in 30 ml of DCM was added over the period of 1 h. Reaction mixture was stirred at 25° C for 24 h. After the completion of the reaction mixture (TLC monitoring), the reaction mixture was evaporated to dryness under reduced pressure. The residue was triturated with n-pentane and decanted the organic layer, dried (Na₂SO₄), filtered and concentrated to give the desired product (2.4 g, 22%).

Example 316: 3-(5-Cyclopent-2-enyl-1 H-inden-2-ylmethoxy)-2,6-difluoro- benzamide

To a solution of 2,6-Difluoro-3- [5-(4,4,5,5-tetramethyl-[1 ,3,2]dioxaborolan-2-yl)-1 H- inden-2-ylmethoxy]-benzamide (0.25 g, 0.56 mmol) in 7 ml of anhydrous DMF and water (3.5 ml) was added trifluoromethanesulfonic acid cyclopent-1-enyl ester (0.37g, 1.70 mmol) and potassium phosphate (0.14 g, 0.60 mmol). The reaction mixture was degassed for 10 minutes followed by addition of dichlorobis(triphenyl phosphine) palladium (II) (0.05 g, 0.08 mmol). The reaction mixture was heated at 80⁰C for 1 h under the nitrogen atmosphere. After the completion of the reaction mixture (TLC monitoring), water (25 ml.) was added and extracted with ethyl acetate (3 x 50 ml_). The combined organics was washed with water, brine, dried (Na₂SO₄), filtered and concentrated. The crude residue was purified over silica gel (230-400 M, 45% EtOAc-Hexane) to get the desired product (0.14 g, 65%) as a white solid. ¹H NMR (DMSO-d₆, 400 MHz): δ 1.22 (m, 2H), 2.01 (m, 2H), 2.88 (m, 2H), 5.68 (s, 2H), 6.43 (s, 1 H), 7.01 (t, J= 9.20 Hz, 1 H), 7.37 (m, 1H), 7.67 (d, J= 8.40 Hz, 1 H), 7.89 (br s, 1 H), 7.94 (s, 1 H), 8.07 (d, J= 8.40 Hz, 1 H) and 8.18 (br s, 1 H). MS ES+ (387.15), HPLC (method II) Rt = 13.74 min.

Example 317: 3-f 5-Cvclopentvl-1 H-inden-2-vlmethoxv)-2.6-dif luoro-benzamide

To a solution of 3-(5-Cyclopent-1-enyl-1 H-inden-2-ylmethoxy)-2,6-difluoro-benzamide (0.05 g, 0.10 mmol) in 5 ml of anhydrous methanol was added Pd-C (10%, 100 mg). The reaction mixture was stirred at 25°C for 48 h under hydrogen atmosphere. The reaction mixture was filtered over the bed of celite and evaporated to dryness under reduced pressure to give the title compound as white solid (0.005 g, 10%). ¹H NMR (DMSO-d₆, 400 MHz); δ 1.22 (m, 2H), 1.67 (m, 4H), 1.80 (m, 2H), 2.07 (m, 2H), 3.20 (m, 1 H), 5.67 (s, 2H), 7.09 (m, 1 H), 7.37 (m, 2H), 7.87 (m, 2H), 8.0 (m, 1H) and 8.18 (br s, 1 H). MS ES+ (389.12), HPLC (method II) Rt = 18.19 min.

Examples 318 to 333 Scheme 44 (Examples 318-320): (a) Lawesson's reagent; (b) substituted bromoacetophenones; (c) LAH, THF; (d) PBr₃, Toluene; (e) 2,6-dufluoro-3-hydroxy benzamide, K₂CO₃, DMF.

X=F, Example 318 X=CF3, Example 319 X=OCF3, Example 320

Ethylthio-oxamate

O

H₂N^₍

OEt S

To the solution of ethyl oxamate (10.0 g, 85.30 mmol) in 120 ml of toluene was added Lawesson's reagent (24.15 g, 59.7 mmol) and the reaction mixture was heated at 12O⁰C for 12 h. After the completion of the reaction mixture (TLC monitoring), the reaction mixture was evaporated to dryness under reduced pressure. The residue was purified by column chromatography on silica (230-400 M) using ethyl acetate/hexane (5:95) as the eluent to provide the title compound (1.8 g, 16%).

4-(4-Trifluoromethyl-phenyl)-thiazole-2-carboxylic acid ethyl ester (Representative example)

To the solution of 2-Bromo-1-(4-trifluoromethyl-phenyl)-ethanone (0.50 g, 0.80 mmol) in 7 ml of ethanol was added ethyl thio-oxamate (0.15 g, 1.14 mmol). The reaction mixture was heated at 80⁰C for 2 h under nitrogen atmosphere. After the completion of the reaction mixture (TLC monitoring), the reaction mixture was concentrated under reduced pressure, water (25 mL) was added and extracted with ethyl acetate (3 x 50 mL). The combined organics was washed with water, brine, dried (Na₂SO₄), filtered and concentrated. The crude residue was purified over silica gel (230-400 M, 2% EtOAc-Hexane) to get the desired product (0.21 g, 76%). The other derivatives were also prepared by the same general method.

[4-(4-Trifluoromethyl-phenyI)-thiazol-2-yl]-methanol

To an ice-cold suspension of LAH (0.056 g, 1.40 mmol) the 8 ml of anhydrous THF was added dropwise a solution of 4-(4-Trifluoromethyl-phenyl)-thiazole-2-carboxylic acid ethyl ester (0.21 g, 0.71 mmol) in the 5 ml of THF. The reaction mixture was stirred at 25⁰C for 1h. After the completion of the reaction mixture (TLC monitoring), cooled the reaction mixture to O⁰C and quenched with 2.5 ml of water followed by the addition of 15% NaOH solution (2 mL) and finally 4 ml of water. The resulting solution was filtered through celite bed and the filtrate was concentrated under reduced pressure. Water (50 mL) was added and extracted with ethyl acetate (3 x 50 mL). The combined organics was washed with water, brine, dried (Na₂SO₄), filtered and concentrated to give the desired product (0.13 g, 70%). The other derivatives were also prepared by the same general method.

2-Bromomethyl-4-(4-trifluoromethyl-phenyl)-thiazole

Br,

To the solution of [4-(4-Trifluoromethyl-phenyl)-thiazol-2-yl]-methanol (0.13g, 0.50 mmol) in 2 ml of toluene was added PBr₃ (0.072 ml, 0.752 mmol) and the reaction mixture was heated at 12O⁰C for 20 min under nitrogen atmosphere. After the completion of the reaction mixture (TLC monitoring), water (25 mL) was added and extracted with ethyl acetate (3 x 50 mL). The combined organics was washed with water, brine, dried (Na₂SO₄), filtered and concentrated. The crude residue was purified over silica gel (230-400 M, 1% EtOAc-Hexane) to get the desired product (0.04 g, 25%). The other derivatives were also prepared by the same general method. Scheme 45 (Example 321): (a) MeOH, H₂SO₄; (b) SnCI₂.2H₂O, EtOH; (c) 2- Benzyloxyacetyl chloride; (d) Lawesson's reagent; (e) BBr₃, DCM; (f) PBr₃, toluene- DMF; (g) 2,6-difluoro-3-hydroxy benzamide, K₂CO₃, DMF.

4-Chloro-3-nitro-benzoic acid methyl ester

To a Solution of 4-chloro-3-nitrobenzoic acid (5.0 g, 24.81 mmol) in 50 ml of methanol was added H₂SO₄ (2 ml, 37.02 mmol) and the reaction mixture was heated at 7O⁰C for 5 h. After completion of the reaction mixture (TLC monitoring), the reaction mixture was evaporated to dryness under reduced pressure. Water (50 ml_) was added and extracted with ethyl acetate (3 x 50 ml_). The combined organics was, dried (Na₂SO₄), filtered and concentrated to give the desired product (5.04 g, 94%).

3-Amino-4-chloro-benzoic acid methyl ester

To a solution of 4-Chloro-3-nitro-benzoic acid methyl ester (5.0 g, 23.19 mmol) in 100 ml of ethanol was added SnCI₂.2H₂O (26.0 g, 115.96 mmol) and the reaction mixture was heated at 8O⁰C for 2 h. After the completion of the reaction mixture (TLC monitoring), the reaction mixture was evaporated to dryness under reduced pressure. Water (100 ml_) was added, basified the reaction mixture with NaOH solution and extracted with hot EtOAc (3 x 250 mL). The combined organics was dried over Na₂SO₄, filtered and concentrated to give the desired product (3.0 g, 69%).

3-(2-Benzyloxy-acetylamino)-4-chloro-benzoic acid methyl ester

A solution of carbonic acid monobenzyl ester (3.50 g, 21.0 mmol) in the 50 ml of DCM and 0.50 ml of DMF was cooled to -78° C followed by addition of oxalyl chloride (11.79 ml, 105 mmol). The resulting reaction mixture was stirred at room temperature for 1 h. After the completion of the reaction mixture (TLC monitoring), concentrated it to give 2-benzyloxyacetyl chloride (3.0 g, 96%). To an ice cold solution of 3-amino-4-chloro-benzoic acid methyl ester in 10 ml of DCM was added triethylamine (2.47 ml, 17.78 mmol) followed by addition of 2-benzyloxyacetyl chloride (3.0 g, 17.78 mmol) in 10 ml of DCM .The reaction mixture was stirred at 25⁰C for 12 hr. After the completion of the reaction mixture (TLC monitoring), the reaction mixture was evaporated to dryness under reduced pressure. The residue was purified by column chromatography on silica (60-120 M) using ethyl acetate/hexane (5:95) as the eluent to provide the title compound (1.70 g, 31%).

3-(2-Benzyloxy-thioacetylamino)-4-chloro-benzoic acid methyl ester

To the solution of 3-(2-Benzyloχy-acetylamino)-4-chloro-benzoic acid methyl ester (1.70 g, 5.10 mmol) in 20 ml of toluene was added Lawesson's reagent (1.03 g, 2.50 mmol) and the reaction mixture was heated at 120° C for 2 h. After the completion of the reaction mixture (TLC monitoring), the reaction mixture was evaporated to dryness under reduced pressure. The residue was purified by column chromatography on silica (60-120 M) using ethyl acetate/hexane (5:95) as the eluent to provide the title compound (1.20 g, 67%).

2-Benzyloxymethyl-benzothiazole-6-carboxylic acid methyl ester

To a solution of 3-(2-Benzyloxy-thioacetylamino)-4-chloro-benzoic acid methyl ester (1.20 g, 3.40 mmol) in the 8 ml of NMP was added NaH (0.12 g, 5.10 mmol) portion wise. The reaction mixture was heated at 16O⁰C for 3 h. After the completion of the reaction mixture (TLC monitoring), the reaction mixture was poured into 150 ml of ice-cold water and extracted with ethyl acetate (3 x 150 mL). The combined organics was dried (Na₂SO₄), filtered and concentrated to give the desired product. (1.07 g, 56%).

2-Hydroxymethyl-benzothiazole-6-carboxylic acid methyl ester

A solution of 2-Benzyloxymethyl-benzothiazole-6-carboxylic acid methyl ester (0.10 g, 0.32 mmol) in 2 ml of DCM was cooled to -78° C followed by addition of BBr₃ (0.06 ml, 0.64 mmol). The reaction mixture was stirred at 25⁰C for 2 h. After the completion of the reaction mixture (TLC monitoring), solution of NaHCO₃ (20 mL) was added at O⁰C and extracted with ethyl acetate (3 x 50 mL). The combined organics was washed with water, brine, dried (Na₂SO₄), filtered and concentrated to get the desired product (0.08 g, Crude yield).

2-Bromomethyl-benzothiazole-6-carboxylic acid methyl ester

To a solution of 2-Hydroxymethyl-benzothiazole-6-carboxylic acid methyl ester (0.08 g, 0.40 mmol) in 5 ml of toluene and 1 ml of DMF was added PBr₃ (0.06 ml, 0.60 mmol). The reaction mixture was heated at 12O⁰C for 20 min under nitrogen atmosphere. After the completion of the reaction mixture (TLC monitoring), water (25 ml_) was added and extracted with ethyl acetate (3 x 50 ml_). The combined organics was washed with water, brine, dried (Na₂SO₄), filtered and concentrated to get the desired product (0.044 g, 36%).

Scheme 46 (Example 322):

Benzo[1,3]dioxoIe-5-carbonitrile

To a solution of 3,4 Dihydroxy benzonitrile (5.0 g, 37.0 mmol) in 20 ml of DMF was added dibromomethane (19.25 g, 110.0 mmol) and potassium carbonate (25.50 g, 184.90 mmol). The reaction mixture was heated at 120⁰C for 2 h under the nitrogen atmosphere. After the completion of the reaction mixture (TLC monitoring), reaction mixture was cooled to room temperature. Water (50 ml) was added to the reaction mixture and extracted the compound with ethyl acetate (3 x 100 mL). The combined organic layers were dried over anhydrous Na₂SO₄, and evaporated to dryness under reduced pressure to give the title compound as yellow solid (5.16 g, 94.8%).

N-Hydroxy-benzo[1,3]dioxole-5-carboxamidine

To a solution of Benzo[1 ,3]dioxole-5-carbonitrile (5.0 g, 33.9 mmol) in EtOH (100 ml_) was added hydroxylamine hydrochloride (4.68 g, 67.90 mmol) and NaOH (2.71 g, 67.9 mmol). The resulting reaction mixture was refluxed for 12 h. After the completion of the reaction (TLC monitoring), the mixture was concentrated, added EtOH and filtered. The filtrate was evaporated under reduced pressure and used as such for the next step (crude yield 4.8 g, 78.68%).

3-Benzo[1 ,3]dioxol-5-yl-5-bromomethyI-[1 ,2,4]oxadiazole

Bromoacetyl bromide (0.22 g, 1.10 mmol) was added to N-Hydroxy- benzo[1 ,3]dioxole-5-carboxamidine (0.40 g, 0.55 mmol) and K₂CO₃ (0.38 g, 0.78 mmol). The reaction mixture was heated at 100⁰C for 15 min. After the completion of the reaction mixture (TLC monitoring), water (25 mL) was added and extracted with ethyl acetate (3 x 50 mL). The combined organics was washed with water, brine, dried (Na₂SO₄), filtered and concentrated. The crude residue was purified over silica gel (60-120 M, 5% EtOAc-Hexane) to get the desired product (0.05 g, 31%).

Scheme 47 (Example 323): (a) Ethyl bromopyruvate, EtOH; (b) LAH, THF; (c) PBr₃, toluene; (d) 2,6-difluoro-3-hydroxy benzamide, K₂CO₃, DMF.

2-(4-Methoxy-phenyl)-thiazole-4-carboxylic acid ethyl ester

To an ice-cold solution of 4-methoxy-thiobenzamide (0.50 g, 2.98 mmol) in ethanol (25 ml) was added triethylamine (0.41 ml, 2.98 mmol) followed by dropwise addition of ethyl bromopyruvate (0.56 ml, 4.40 mmol). The reaction mixture was heated at 65⁰C for 12 h. After the completion of the reaction mixture (TLC monitoring), the reaction mixture was evaporated to dryness under reduced pressure, water (25 mL) was added and extracted with ethyl acetate (3 x 50 mL). The combined organics was washed with water, brine, dried (Na₂SO₄), filtered and concentrated. The crude residue was purified over silica gel (60-120 M, 10% EtOAc-Hexane) to get the desired product (0.38 g, 48%).

[2-(4-Methoxy-phenyl)-thiazol-4-yl]-methanol

To an ice-cold suspension of LAH (0.08 g, 2.07 mmol) in 10 ml of anhydrous THF was added a solution of 2-(4-Methoxy-phenyl)-thiazole-4-carboxylic acid ethyl ester (0.26 g, 0.98 mmol) in 5 ml of THF. The reaction mixture was heated up to 6O⁰C for 1 h. After the completion of the reaction mixture (TLC monitoring), the reaction mixture was cooled to O⁰C, water (2.0 ml) was added followed by the addition of 15% NaOH solution (2 mL) and finally 4 ml of water. The resulting solution was filtered through celite bed and concentrated under reduced pressure; water (25 mL) was added and extracted with ethyl acetate (3 x 50 mL). The combined organics was washed with water, brine, dried (Na₂SO₄), filtered and concentrated to give the desired product (0.14 g, 64%).

4-Bromomethyl-2-(4-methoxy-phenyl)-thiazole

To a solution of [2-(4-Methoxy~phenyl)-thiazol-4~yl]-methanol (0.12 g, 0.50 mmol) in 3 ml of toluene was added PBr₃ (0.078 ml, 0.813 mmol) and the reaction mixture was heated at 12O⁰C for 20 min under nitrogen atmosphere. After the completion of the reaction mixture (TLC monitoring), water (25 mL) was added and extracted with ethyl acetate (3 x 50 mL). The combined organics was washed with water, brine, dried (Na₂SO₄), filtered and concentrated to get the desired product (0.13 g, 84%).

Scheme 48 (Example 324): (a) Propionic anhydride, K₂CO₃; (b) NBS, AIBN, CCI₄; (c) 2,6-difluoro-3-hydroxy benzamide, K₂CO₃, DMF.

3-(4-Chloro-phenyl)-5-ethyI-[1,2,4]oxadiazole

Propionic anhydride (0.75 ml_, 5.79 mmol) was added to 4-Chloro-N-hydroxy- benzamide (0.50 g, 2.89 mmol) and K₂CO₃ (2.0 g, 14.48 mmol). The reaction mixture was heated at 100⁰C for 30 min. After the completion of the reaction mixture (TLC monitoring), the reaction mixture was cooled to O⁰C, added water (25 ml_) and extracted with ethyl acetate (3 x 50 mL). The combined organics was washed with water, brine, dried (Na₂SO₄), filtered and concentrated. The crude residue was purified over silica gel (60-120 M, 5% EtOAc-Hexane) to get the desired product (0.29 g, 48%).

5-(1 -Bromo-ethyl)-3-(4-chloro-phenyl)-[1 ,2,4]oxadiazole

To a solution of 3-(4-Chloro-phenyl)-5-ethyl-[1 ,2,4]oxadiazole (0.29 g, 1.38 mmol) in CCI₄ (10 mL) was added NBS (0.24 g, 1.38 mmol) and AIBN ( 0.02 g, 0.0001 mmol). The reaction mixture was heated at 100⁰C for 2 h under nitrogen atmosphere. After the completion of the reaction mixture (TLC monitoring), the reaction mixture was evaporated to dryness under reduced pressure and the residue was purified by column chromatography on silica (230-400 M) using 1% ethyl acetate/hexane as eluent to give the desired product (0.12 g, 30%).

Scheme 49 (Example 325): (a) PBr₃, toluene; (b) 2,6-difluoro-3-hydroxy benzamide, K₂CO₃, DMF.

4-Bromomethyl-5-methyl-2-phenyI-2H-[1 ,2,3]triazole

To a solution of (5-Methyl-2-phenyl-2H-[1 ,2,3]triazol-4-yl)-methanol (0.25 g, 1.30 mmol) in 10 ml of toluene was added PBr₃ (0.53 g, 1.90 mmol) and the reaction mixture was heated at 12O⁰C for 20 min under nitrogen atmosphere. After the completion of the reaction mixture (TLC monitoring), water (25 ml_) was added and extracted with ethyl acetate (3 x 50 ml_). The combined organics was washed with water, brine, dried (Na₂SO₄), filtered and concentrated to get the desired product (0.30 g, 90%) as a yellow solid.

Scheme 50 (Example 326): (a) CuCN, Pyridine; (b) Hydroxylamine hydrochloride, ethanol; (c) chloroacetyl chloride, K₂CO₃; (d) 2,6-difluoro-3-hydroxy benzamide, K₂CO₃, DMF.

Thiazole-2-carbonitrile

-N

[T W>-^CN

To a solution of 2-bromothiazole (1.0 g, 6.09 mmol) in 4 ml of pyridine was added CuCN (1.09 g, 12.19 mmol). The reaction mixture was heated to 15O⁰C for 3 h. After the completion of the reaction, pH was adjusted to 3-4 with 1 N HCI solution and extracted with ethyl acetate (3 x 50 mL). The combined organics was washed with water, brine, dried (Na₂SO₄), filtered and concentrated to get the desired product (0.42 g, 63%). N-Hydroxy-thiazole-2-carboxamidine

To a solution of thiazole-2-carbonitrile (0.42 g, 3.80 mmol) in EtOH (20 mL) was added hydroxylamine hydrochloride (0.53 g, 7.60 mmol) and pyridine (0.27 g, 3.40 mmol). The resulting reaction mixture was refluxed for 15h. After the completion of the reaction (TLC monitoring), the mixture was concentrated, added EtOH and filtered. The filtrate was evaporated under reduced pressure and used as such for the next step (crude yield 0.50 g, 91% crude yield).

5-Chloromethyl-3-thiazol-2-yl-[1,2,4]oxadiazoIe

Chloroacetyl Chloride (5.0 mL, 44.5 mmol) was added to N-Hydroxy-thiazole-2- carboxamidine (0.50 g, 3.49 mmol) and K₂CO₃ (1.0 g, 7.20 mmol). The reaction mixture was heated at 100⁰C for 15 min. After the completion of the reaction mixture (TLC monitoring), water (25 mL) was added and extracted with ethyl acetate (3 x 50 mL). The combined organics was washed with water, brine, dried (Na₂SO₄), filtered and concentrated. The crude residue was purified over silica gel (230-400 M, 10% EtOAc-Hexane) to get the desired product (0.18 g, 25%) as a white solid.

Scheme 51 (Example 327): (a) acetyl chloride, Et₃N; (b) Lawesson's reagent; (c) Br₂, DCM; (d) NBS, AIBN, CCI₄; (e) 2,6-difluoro-3-hydroxy benzamide, K₂CO₃, DMF.

N-(4-Phenoxy-phenyl)-acetamide

To an ice-cold solution of 4-phenoxy-phenylamine (1.0 g, 5.39 mmol) in 10 ml of DCM was added triethylamine (0.90 ml, 5.93 mmol) followed by acetyl chloride (0.50 g, 6.47mmol). The reaction mixture was stirred at 25⁰C for 2 h. After the completion of the reaction mixture (TLC monitoring), water was added extracted with DCM (3 x 50 ml_). The combined organics was dried (Na₂SO₄), filtered and concentrated to get the desired product (1.20 g, crude yield).

N-(4-Phenoxy-phenyl)-thioacetamide

To a solution of N-(4-phenoxy-phenyl)-acetamide (1.20 g, 5.28 mmol) in 10 ml of toluene was added Lawesson's reagent (1.50 g, 3.70 mmol). The reaction mixture was heated at 12O⁰C for 2 h. After the completion of the reaction (TLC monitoring), the reaction mixture was evaporated to dryness under reduced pressure. The residue was purified by column chromatography on silica (60-120 M) using ethyl acetate/hexane (5:95) as the eluent to provide the title compound (0.78 g, 60.7%).

2-Methyl-6-phenoxy-benzothiazole

To an ice-cold solution of N-(4-phenoxy-phenyl)-thioacetamide (0.78 g, 3.20 mmol) in 10 ml of DCM was added Br₂ (0.32 ml, 6.40 mmol) dropwise. The reaction mixture was heated at 45⁰C for 2 h. After the completion of the reaction (TLC monitoring), the reaction mixture was evaporated under reduced pressure. The residue was basified with NH₄OH solution and extracted with ethyl acetate The combined organics were, dried, (Na₂SO₄), filtered and concentrated. The residue was purified by column chromatography on silica (230-400 M) using ethyl acetate/hexane (3:97) as the eluent to provide the title compound (0.08 g, 10.3%).

2-Bromomethyl-6-phenoxy-benzothiazole

To a solution of 2-methyl-6-phenoxy-benzothiazoIe (0.06 g, 0.24 mmol) in 5 ml of CCI₄ was added NBS (0.039 g, 0.22 mmol) and AIBN (0.004 g, 0.024 mmol). The reaction mixture was heated at 100⁰C for 2 h under nitrogen atmosphere. After the completion of the reaction mixture (TLC monitoring), the reaction mixture was evaporated to dryness under reduced pressure and the residue was purified by column chromatography on silica (230-400M) using 1% ethyl acetate/hexane eluent to give the desired product (0.005g, 6.3%).

Scheme 52 (Example 330): (a) NBS, AIBN, CCI₄; (b) 2,6-difluoro-3-hydroxy benzamide, K₂CO₃, DMF.

7-Bromomethyl-quinoline

To a solution of 7-methylquinoline (0.10 g, 0.70 mmol) in 5 ml of CCI₄ was added NBS (0.14 g, 0.77 mmol) and AIBN (0.025 g, 0.15 mmol). The reaction mixture was heated at 100⁰C for 2 h under nitrogen atmosphere. After the completion of the reaction mixture (TLC monitoring), the reaction mixture was evaporated to dryness under reduced pressure and the residue was purified by column chromatography on silica (230-400M) using 1 % ethyl acetate/hexane eluent to give the desired product (0.09Og, 58%).

Examples 318-333 (Table R) The compounds of Examples 318-333 were synthesised according to the following general procedure: To a solution of reactant (A) in anhydrous DMF was added 2,6- Difluoro-3-hydroxy-benzamide (B) and potassium carbonate (C). The reaction mixture was stirred at 25 °C for 24 h under nitrogen atmosphere. The reaction mixture was evaporated to dryness under reduced pressure and the residue was purified by column chromatography on silica using ethyl acetate/hexane as the eluent to provide the product compound. Table R

328 329

3-[3-(4-Difluoromethoxy-3-methoxy-phenyl)- 3-[3-(4-Chloro-3-nitro-phenyl)-

[1 ,2,4]oxadiazol-5-ylmethoxy]-2,6-difluoro- [1 ,2,4]oxadiazol-5-ylmethoxy]-2,6-difluoro- benzamide benzamide

(a) 2,6-difluoro-3-hydroxy benzamide, K₂CO₃, DMF (a) 2,6-difluoro-3-hydroxy benzamide, K₂CO₃, DMF

5-chloromethyl-3-(4-difluoromethoxy-3-methoxy- 5-chloromethyl-3-(4-chloro-3-nitro-phenyl)- phenyl)-[1 ,2,4]oxadiazole [1 ,2,4]oxadiazole

0.10 g, 0.34 mmol; 0.059 g, 0.34 mmol; 0.16 g, 0.15 g, 0.54 mmol; 0.085 g, 0.49 mmol; 0.26 1.20 mmol; 2 ml g, 1.90 mmol; 2 ml

50:50 50:50

60-120 M 60-120 M

0.035 g, 23%, white solid 0.06 g, 26%, white solid δ 3.92 (s, 3H), 5.70 (s, 2H), 7.14 (m, 1 H), 7.22 (s, δ 5.76 (s, 2H), 7.13 (m, 1 H), 7.40 (m, 1H), 1 H), 7.40 (m, 2H), 7.65 (m, 2H), 7.91 (br s, 1 H) 7.90 (br s, 1 H), 8.01 (d, J= 8.40 Hz, 1H), 8.18 and 8.19 (br s, 1 H) (br s, 1 H), 8.29 (m, 1 H) and 8.61 (s, 1 H) 428.27 411.15 8, 15.96 8, 16.20

Scheme 53: (a) Thioacetamide, DMF; (b) NBS, AIBN, CCI₄, (c) 2,6-difluoro-3- hydroxybenzamide, K₂CO₃, DMF (d) Zn, AcOH (e) BBr_3, DCM.

4-(3-Methoxy-phenyl)-2-methyl-thiazole

A mixture of thioacetamide (8.0 g, 106.0 mmol) and 2-bromo-1-(3-methoxy-phenyl)- ethanone (2.0 g, 8.81 mmol) was heated at 14O⁰C for 6 h under nitrogen atmosphere. After completion of the reaction mixture (TLC monitoring), water (50 ml.) was added and extracted with ethyl acetate (3 x 50 mL). The combined organics was washed with water, brine, dried (Na₂SO₄), filtered and concentrated. The crude residue was purified over silica gel (230-400 M, 2% EtOAc-Hexane) to get the desired product (1.5 g, 83%).

5-Bromo-2-bromomethyl-4-(3-methoxy-phenyl)-thiazole

To a solution 4-(3-methoxy-phenyl)-2-methyl-thiazole (1.50, 7.30 mmol) in the 20 ml of CCI₄ was added NBS (2.60 g, 14.60 mmol) and AIBN (0.12 g, 0.73 mmol). The reaction mixture was heated at 100⁰C for 2 h under nitrogen atmosphere. After completion of the reaction mixture (TLC monitoring), the reaction mixture was evaporated to dryness under reduced pressure and the residue was purified by column chromatography on silica (230-400 M) using 2% ethyl acetate/hexane as a eluent to give the desired product (1.20 g, 45%).

3-[5-Bromo-4-(3-methoxy-phenyl)-thiazol-2-ylmethoxy]-2,6-difluoro-benzamide

To a solution of 5-Bromo-2-bromomethyl-4-(3-methoxy-phenyl)-thiazole (0.80 g, 2.20 mmol) in 5 ml of anhydrous DMF was added 2,6-Difluoro-3-hydroxy-benzamide (0.38 g, 2.20 mmol) and potassium carbonate (1.06 g, 7.70 mmol). The reaction mixture was stirred at 25°C for 24 h under nitrogen atmosphere. The reaction mixture was evaporated to dryness under reduced pressure and the residue was purified by column chromatography on silica (60-120 M) using ethyl acetate/hexane (50:50) as the eluent to provide the title compound as white solid (0.50 g, 49%).

2,6-Difluoro-3-[4-(3-methoxy-phenyl)-thiazol-2-ylmethoxy]-benzamide

To the solution of 3-[5-bromo-4-(3-methoxy-phenyl)-thiazol-2-ylmethoxy]-2,6-difluoro- benzamide (0.50 g, 1.10 mmol) in the 10 ml of acetic acid was added Zn dust (0.50 g, w/w). The reaction mixture was heated at 120⁰C for 1 h. After the completion of the reaction mixture (TLC monitoring), water (50 ml.) was added and pH was adjusted to 8-9 with NaOH solution and extracted with ethyl acetate (3 x 100 mL). The combined organics was washed with water, brine, dried (Na₂SO₄), filtered and concentrated to get the desired product (0.22 g, 53%).

Example 334: 2,6-Difluoro-3-[4-(3-hydroxy-phenyl)-thiazol-2-ylmethoxy]- benzamide

A solution of 2,6-Difluoro-3-[4-(3-methoxy-phenyl)-thiazol-2-ylmethoxy]-benzamide (0.20 g, 0.53 mmol) in 15 ml of DCM was cooled to -78⁰C followed by addition of BBr₃ (0.20 ml, 2.14 mmol). The reaction mixture was stirred at 25⁰C for 2 h. After the completion of the reaction mixture (TLC monitoring), solution of NaHCO₃ (20 mL) was added at O⁰C and extracted with ethyl acetate (3 x 50 mL). The combined organics was washed with water, brine, dried (Na₂SO₄), filtered and concentrated. The residue was purified by column chromatography on silica (60-120 M) using ethyl acetate/hexane (50:50) as the eluent to provide the title compound as white solid (0.065 g, 33%). ¹H NMR (DMSO-d₆, 400 MHz): δ 5.60 (s, 2H), 6.74 (m, 1 H), 7.10 (m, 1H), 7.24 (m, 1 H), 7.37-7.45 (m, 3H), 7.90 (br s, 1H), 8.10 (s, 1 H), 8.17 (br s, 1 H) and 9.55 (s, 1 H). MS ES+(362.99), HPLC (method II) Rt = 14.95 min.

Minimum Inhibitory Concentration (MIC) Testing

Compounds of this invention were tested for antimicrobial activity by susceptibility testing in liquid media. MICs for compounds against each strain were determined by a broth microdilution method according to the National Committee for Clinical Laboratory Standards (NCCLS) guidelines. (NCCLS. 2000. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically — fifth edition. Approved standard M7-A5. NCCLS, Wayne, Pa.)

Briefly, test compounds are prepared in 100 μl of 1.6% DMSO solution in multiwell plates. Several bacterial colonies from a freshly streaked plate are transferred to an appropriate rich broth, such as Mueller Hinton. The cell suspension is adjusted to an optical density of 0.09 and further diluted 1 :100 with warm 2x broth. This cell suspension is dispensed into the wells containing compound solution so that the final volume is 200 μl. The plates are incubated overnight (16-20 hours) at 37⁰C and turbidity is scored by eye and quantified spectrophotometrically. The MIC is defined as the lowest concentration inhibiting visible growth. Compounds of the current invention were found to have antimicrobial activity in the MIC assay described above. Results

Table 1 shows the Minimal Inhibitory Concentration (MIC) of the Examples against Bacillus subtilis 168_CA. Activities were scored as 'A' if the MIC was <8 micrograms/ml, 'B' if the MIC was 16 to 64 micrograms/ml and 'C if the MIC was greater than 64 micrograms/ml.

Table 1 Bacillus subtilis MICs

Table 1 (continued) Bacillus subtilis MICs

Some of the compounds of the Examples were also tested for activity against the pathogenic organism Staphylococcus aureus ATCC29213. Table 2 shows the MICs of the Examples against Staphylococcus aureus. Activities were again scored as 'A' if the MIC was <8 micrograms/ml, 'B' if the MIC was 16 to 64 micrograms/ml and 'C if the MIC was greater than 64 micrograms/ml.

Some of the Examples were also tested for activity against other bacterial species. Table 3 shows the MICs of the Examples against various bacterial species. Activities were again scored as 'A' if the MIC was <8 micrograms/ml, 'B' if the MIC was 16 to 64 micrograms/ml and 'C if the MIC was greater than 64 micrograms/ml.

Table 3 MICs against various bacteria

Some of the Examples were also tested for activity against staphylococcal clinical isolates. Table 4 shows the MICs of the examples against various clinical isolates. Activities were again scored as 'A' if the MIC was <8 micrograms/ml, 'B' if the MIC was 16 to 64 micrograms/ml and 'C if the MIC was greater than 64 micrograms/ml.

S, susceptible; I, intermediate; R, resistant ² Van, vancomycin; LZD, linezolid; Tet, tetracycline; MI, minocycline; CC, clindamycin, SXT, trimethoprim/sulfamethoxazole; Doxy, doxycycline; iMLS, inducible macrolide-lincosamide- streptogramin B resistance; TMP, trimethoprim; Rif, rifampin

Some of the Examples were also tested for activity in a mouse Staphylococcus aureus septicaemia model of infection. Table 5 shows the survival at day 7 of infected mice treated with a single intraperitoneal dose of 100 mg/kg of each Example at 1 hour after intraperitoneal inoculation with a lethal dose of Staphylococcus aureus.

Table 5 Murine Survival

Claims

Claims:

1. The use of a compound which is a substituted benzamide or pyridylamide of formula (I) or a salt, hydrate, or solvate thereof, in the manufacture of a medicament for use in treating bacterial infection:

wherein

R represents hydrogen or 1 , 2 or 3 optional substituents;

W is =C(R0- or =N-;

R₁ is hydrogen or an optional substituent and R₂ is hydrogen, methyl, or fluorine; or R₁ and R₂ taken together are -CH₂-, -CH₂CH₂-, -O-, or, in either orientation, -0-CH₂- or -OCH₂CH₂-;

R₃ is a radical of formula -(Alk¹)_m-(Z)_p-(Alk²)_n-Q wherein

m, p and n are independently 0 or 1, provided that at least one of m, p and n is 1,

Z is -O-, -S-, -S(O)-, -S(O₂)-, -NH-, -N(CH₃)-, -N(CH₂CH₃)-,

-C(=O)-, -O-(C=O)-, -C(=O)-0-, or an optionally substituted divalent monocyclic carbocyclic or heterocyclic radical having 3 to 6 ring atoms; or an optionally substituted divalent bicyclic heterocyclic radical having 5 to 10 ring atoms;

AIk¹ and AIk² are optionally substituted C₁-C₆ alkylene, C₂-C₆ alkenylene, or C₂-C₆ alkynylene radicals, which may optionally terminate with or be interrupted by -O-, -S-, -S(O)-, -S(O₂)-, -NH-, -N(CH₃)-, Or -N(CH₂CH₃)-; and Q is hydrogen, halogen, nitrile, or hydroxyl or an optionally substituted monocyclic carbocyclic or heterocyclic radical having 3 to 6 ring atoms; or an optionally substituted bicyclic heterocyclic radical having 5 to 10 ring atoms.

2. The use as claimed in claim 1 wherein the compound has formula (IA)

wherein R₄ and R₅ are independently fluoro or chloro, or one of R₄ and R₅ is hydrogen while the other is fluoro or chloro, and R₁, R₂ and R₃ are as defined in claim 1.

3. The use as claimed in claim 1 wherein the compound has formula (IB)

wherein R₂ is hydrogen, methyl, or fluoro; and R₃ is as defined in claim 1

4. The use as claimed in any of claims 1 to 3 wherein R₁ and R₂ are hydrogen.

5. The use as claimed in any of the preceding claims wherein p is 0, and m and/or n is 1.

6. The use as claimed in any of claims 1 to 4 wherein p is 1 , and Z is an optionally substituted heteroaryl radical having 3 to 6 ring atoms or an optionally substituted bicyclic heteroaryl radical having 5 to 10 ring atoms, which is linked to the -(Alk¹)_m- part of R₃ and to the -(Alk²)_n-Q part of R₃ via ring carbon or nitrogen atoms.

7. The use as claimed in claim 6 wherein the divalent radical Z is selected from the following, optionally substituted, in either orientation:

8. The use as claimed in claim 6 wherein the divalent radical Z is selected from the following, optionally substituted, in either orientation:

9. The use as claimed in claim 6 wherein the divalent radical Z is selected from the following, optionally substituted, in either orientation:

10. The use as claimed in any of claims 1 to 4 wherein p is 1 , and Z is an optionally substituted monocyclic non-aromatic carbocyclic or heterocyclic radical having 3 to 6 ring atoms or an optionally substituted bicyclic non-aromatic carbocyclic or heterocyclic having 5 to 10 ring atoms, which is linked to the -(Alk¹)_m- part of R₃ and to the -(Alk²)_π-Q part of R₃ via ring carbon or nitrogen atoms.

11. The use as claimed in claim 10 wherein the divalent radical Z is selected from the following, optionally substituted, in either orientation:

12. The use as claimed in any of claims 6 to 11 wherein Q is hydrogen.

13. The use as claimed in any of claims 1 to 11 wherein Q is a radical selected from any of the divalent radicals specified in any of claims 6 to 9 with one of the unsatisfied valencies thereof satisfied with hydrogen or an optional substituent.

14. The use as claimed in any of claims 6 to 13 wherein n is 0.

15. The use as claimed in any of claims 6 to 14 wherein m is 0.

16. The use as claimed in any of the preceding claims wherein the length of the radical R₃ does not exceed the length of an unbranched saturated hydrocarbon chain of 14 carbon atoms.

17. The use as claimed in any of claims 1 to 15 wherein the length of the radical R₃ is equivalent to that of an unbranched saturated hydrocarbon chain of from 6 to 12, or 9 to 12 carbon atoms.

18. The use as claimed in any of the preceding claims wherein AIk¹ and AIk², when present, are optionally substituted straight chain C₁-C₆ alkylene, C₂-C₆ alkenylene, or C₂-C₆ alkynylene radicals, each of which may optionally terminate with or be interrupted by -O-, -S-, -S(O)-, -S(O₂)-, -NH-, -N(CH₃)-, or -N(CH₂CH₃)-, - C(=O)-, -0-(C=O)-, -C(=O)-O-.

19. The use as claimed in any of the preceding claims wherein any optional substituents R and any optional substituents present in AIk¹, AIk², Z and Q are selected from methyl, ethyl, cyclopropyl, oxo, hydroxyl, halogen, cyano, acetyl, amino, methylamino, dimethylamino, acetylamino, carbamate and CH₂OH.

20. The use as claimed in claim 2 wherein R₂ is hydrogen and R₃ is a radical selected from those of formulae A-H:

A B C

D F

H wherein Q is as defined in claim 1 , and wherein any unsubstituted ring carbon is optionally substituted.

21. The use as claimed in claim 20 wherein Q is hydrogen or optionally substituted phenyl.

22. The use as claimed in claim 2 wherein R₂ is hydrogen, and R₃ is optionally substituted quinolin-2-yl, benzothiazol-2-yl, thiazolopyridin-2-yl , thiazol-2-yl, thiazol- 4-yl, thiazol-5-yl, oxazol-2-yl, oxazol-4-yl, oxadiazoI-3-yl or oxadiazol-5-yl.

23 The use as claimed in claim 22 wherein R₃ is substituted by optionally substituted phenyl.

24. The use as claimed in any of claims 20 to 22 wherein any optional substituents in R₃ are selected from methyl, -OCH₃, -CF₃, -OCF₃, ethyl, cyclopropyl, oxo, hydroxyl, -F, -Cl, -Br, cyano, acetyl, amino, methylamino, dimethylamino, acetylamino, carbamate, -CONH₂, nitro, -COOH and -CH₂OH.

25. A compound which is a substituted benzamide or pyridylamide of formula (IC) or a salt, hydrate or solvate thereof:

wherein W is =C(R₁)- or =N-;

Ri is hydrogen or an optional substituent and R₂ is hydrogen, methyl, or fluoro; or R₁ and R₂ taken together are -CH₂-, -CH₂CH₂-, -O-, or, in either orientation, -O-CH₂-or -OCH₂CH₂-;

R₄ and R₅ are independently fluoro or chloro, or one of R₄ and R₅ is hydrogen while the other is fluoro or chloro; 75

D

26. A compound as claimed in claim 25 wherein W is =CH- and R₂ is hydrogen.

27. A compound as claimed in claim 25 or claim 26 wherein Q in radical R₃ is hydrogen or optionally substituted phenyl.

28 A compound as claimed in claim 25 or claim 26 wherein R₃ is optionally substituted quinolin-2-yl, benzothiazol-2-yl, thiazol-2-yl, thiazol-4-yl, thiazol-5-yl, oxadiazol-3-yl, oxadiazol-5-yl, oxazol-2-yl, oxazol-4-yl, oxazoI-5-yl or thiazolopyridin- 2-yl.

29 A compound as claimed in claim 28 wherein R₃ is substituted by optionally substituted phenyl.

30. A compound as claimed in any of claims 25 to 29 wherein any optional substituents in R₃ are selected from methyl, -OCH₃, -CF₃, -OCF₃, ethyl, cyclopropyl, oxo, hydroxyl, -F, -Cl, -Br, cyano, acetyl, amino, methylamino, dimethylamino, acetylamino, carbamate, -CONH₂, nitro, -COOH and -CH₂OH.

31. A compound which is a pyridylamide of formula (ID) or a salt, hydrate or solvate thereof:

wherein R₂ is hydrogen, methyl, or fluoro; and R₃ is as defined in claim 25.

32. A compound as claimed in claim 31 wherein R₂ is hydrogen.

33. A compound as claimed in claim 31 or claim 32 wherein Q in radical R₃ is hydrogen or optionally substituted phenyl.

34 A compound as claimed in claim 31 or claim 32 wherein R₃ is optionally substituted quinolin-2-yl, benzothiazol-2-yl, thiazol-2-yl, thiazol-4-yl, thiazol-5-yl, oxadiazol-3-yl, oxadiazol-5-yl, oxazol-2-yl, oxazol-4-yl, oxazol-5-yl or thiazolopyridin- 2-yl.

35 A compound as claimed in claim 34 wherein R₃ is substituted by optionally substituted phenyl.

36. A compound as claimed in any of claims 31 to 25 wherein any optional substituents in R₃ are selected from methyl, -OCH₃, -CF₃, -OCF₃, ethyl, cyclopropyl, oxo, hydroxyl, -F, -Cl, -Br, cyano, acetyl, amino, methylamino, dimethylamino, acetylamino, carbamate, -CONH₂, nitro, -COOH and -CH₂OH.

37. A pharmaceutical composition comprising a compound as claimed in any of claims 25 to 36, together with a pharmaceutically acceptable carrier.

38. An antibacterial composition comprising a compound as claimed in any of claims 25 to 36 in an amount effective to inhibit bacterial growth, together with a pharmaceutically acceptable carrier.

39. A compound as defined in any of claims 1 to 36, for use in a method of treatment of the human or animal body.

40. A compound as defined in any of claims 1 to 36, for use in treating bacterial infection.

41. A method of treating bacterial infection in a subject suffering such infection comprising administering to the subject an amount of a compound as defined in any of claims 1 to 36 sufficient to inhibit bacterial growth.

42. A method of treating bacterial contamination of a substrate comprising applying to the site of such contamination an amount of a compound as defined in any of claims 1 to 36 sufficient to inhibit bacterial growth.