WO2007099556A2 - Conjunctival tissue system - Google Patents
Conjunctival tissue system Download PDFInfo
- Publication number
- WO2007099556A2 WO2007099556A2 PCT/IN2007/000075 IN2007000075W WO2007099556A2 WO 2007099556 A2 WO2007099556 A2 WO 2007099556A2 IN 2007000075 W IN2007000075 W IN 2007000075W WO 2007099556 A2 WO2007099556 A2 WO 2007099556A2
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- conjunctival
- tissue
- cells
- serum
- tissue system
- Prior art date
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Abstract
Description
Claims
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NZ570746A NZ570746A (en) | 2006-02-24 | 2007-02-23 | Conjunctival tissue system |
CA002642947A CA2642947A1 (en) | 2006-02-24 | 2007-02-23 | Conjunctival tissue system |
AU2007220122A AU2007220122B2 (en) | 2006-02-24 | 2007-02-23 | Conjunctival tissue system |
JP2008555952A JP2009527250A (en) | 2006-02-24 | 2007-02-23 | Conjunctival tissue system |
EP07736536A EP1987133A2 (en) | 2006-02-24 | 2007-02-23 | Conjunctival tissue system |
IL193597A IL193597A0 (en) | 2006-02-24 | 2008-08-21 | Conjunctival tissue system |
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IN267/MUM/2006 | 2006-02-24 | ||
IN267MU2006 | 2006-02-24 |
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WO2007099556A2 true WO2007099556A2 (en) | 2007-09-07 |
WO2007099556A3 WO2007099556A3 (en) | 2007-10-18 |
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US (1) | US8338175B2 (en) |
EP (1) | EP1987133A2 (en) |
JP (1) | JP2009527250A (en) |
KR (1) | KR20080097226A (en) |
CN (1) | CN101432031A (en) |
AU (1) | AU2007220122B2 (en) |
CA (1) | CA2642947A1 (en) |
IL (1) | IL193597A0 (en) |
NZ (1) | NZ570746A (en) |
RU (1) | RU2008137975A (en) |
WO (1) | WO2007099556A2 (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2008131973A2 (en) * | 2007-04-26 | 2008-11-06 | Medinnova As | Transplant storage |
WO2010049181A1 (en) * | 2008-10-29 | 2010-05-06 | Oslo University Hospital | Storage of conjuctival cells |
Families Citing this family (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8372437B2 (en) | 2006-08-17 | 2013-02-12 | Mimedx Group, Inc. | Placental tissue grafts |
EP3189731B1 (en) | 2007-09-07 | 2020-01-29 | MiMedx Group, Inc. | Placental tissue grafts and improved methods of preparing and using the same |
EP2309003A1 (en) * | 2009-09-18 | 2011-04-13 | Bioftalmik, S.L. | Method for the diagnosis of limbal stem cell deficiency |
US8883210B1 (en) | 2010-05-14 | 2014-11-11 | Musculoskeletal Transplant Foundation | Tissue-derived tissuegenic implants, and methods of fabricating and using same |
US9352003B1 (en) | 2010-05-14 | 2016-05-31 | Musculoskeletal Transplant Foundation | Tissue-derived tissuegenic implants, and methods of fabricating and using same |
US10130736B1 (en) | 2010-05-14 | 2018-11-20 | Musculoskeletal Transplant Foundation | Tissue-derived tissuegenic implants, and methods of fabricating and using same |
CN102266587B (en) * | 2011-07-20 | 2013-12-04 | 山东省眼科研究所 | Preparation method of recombinant eye conjunctival epithelial diaphragm containing goblet cells |
US8497124B2 (en) * | 2011-12-05 | 2013-07-30 | Factor Bioscience Inc. | Methods and products for reprogramming cells to a less differentiated state |
DK3260140T3 (en) | 2011-12-05 | 2021-04-19 | Factor Bioscience Inc | METHODS AND CELL TRANSFER PROCEDURES |
WO2014143943A1 (en) | 2013-03-15 | 2014-09-18 | NuTech Spine, Inc. | Preparations containing hepatocyte growth factor and hyaluronic acid, and methods of making and using same |
WO2016187413A1 (en) | 2015-05-21 | 2016-11-24 | Musculoskeletal Transplant Foundation | Modified demineralized cortical bone fibers |
KR101645901B1 (en) * | 2015-12-31 | 2016-08-04 | 가톨릭대학교 산학협력단 | Method for culturing limbal stem cell using amniotic membrane slide scaffold |
CN108339147B (en) * | 2017-01-24 | 2021-05-07 | 沈阳何氏眼产业集团有限公司 | Adhesive for vitreous or retinal surgical incision and adhesive system |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20040009590A1 (en) * | 2002-03-29 | 2004-01-15 | Donald Tan | Method for growth of human conjunctival tissue equivalents for research, clinical ocular surface transplantation and tissue engineering |
US20050186672A1 (en) * | 2004-01-27 | 2005-08-25 | Reliance Life Sciences Pvt. Ltd. | Tissue system with undifferentiated stem cells derived from corneal limbus |
Family Cites Families (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
IT1255657B (en) | 1992-05-29 | 1995-11-09 | DIFFERENTIATED CONJUNCTIVAL MUCOSA FABRIC CULTURES, PROCEDURES FOR THEIR PREPARATION AND SUPPORTS FOR THEIR USE. | |
US6152142A (en) | 1997-02-28 | 2000-11-28 | Tseng; Scheffer C. G. | Grafts made from amniotic membrane; methods of separating, preserving, and using such grafts in surgeries |
DE69822724T2 (en) * | 1997-08-07 | 2005-03-17 | Cancel, Richard | High purity alkyl 2-cyanoacrylates |
AU4860900A (en) | 1999-06-02 | 2000-12-18 | Lifebank Services, L.L.C. | Methods of isolation, cryopreservation, and therapeutic use of human amniotic epithelial cells |
JP4397082B2 (en) * | 1999-12-09 | 2010-01-13 | 有限会社ジャパン・オフサルミック・コンサルタンツ | Cell piece for transplantation and method for producing the same |
US20020039788A1 (en) | 2000-02-29 | 2002-04-04 | Isseroff Roslyn R. | Corneal epithelial graft composites |
US6921665B2 (en) | 2000-11-27 | 2005-07-26 | Roslin Institute (Edinburgh) | Selective antibody targeting of undifferentiated stem cells |
US7347876B2 (en) | 2001-04-25 | 2008-03-25 | Ray Jui-Fang Tsai | Method for expansion of epithelial stem cells |
ITRM20010476A1 (en) | 2001-08-03 | 2003-02-03 | Idi Irccs | HUMAN CORNEAL EPITHELIUM SHEETS RECONSTRUCTED AND METHOD FOR THEIR OBTAINING. |
WO2003030959A1 (en) | 2001-10-06 | 2003-04-17 | Btg International Limited | Corneal repair device |
ITUD20020092A1 (en) | 2002-04-30 | 2002-07-30 | Univ Degli Studi Udine | METHOD FOR THE IDENTIFICATION AND ISOLATION OF STEM CELLS OF A HUMAN FABRIC |
JP2005218585A (en) * | 2004-02-04 | 2005-08-18 | National Institute For Materials Science | Medical material and production method thereof |
JPWO2006003818A1 (en) * | 2004-06-30 | 2008-04-17 | アルブラスト株式会社 | Corneal epithelial sheet and method for producing the same |
ZA200602094B (en) | 2006-01-16 | 2007-11-28 | Reliance Life Sciences Pvt Ltd | Device for culturing and transporting cells |
US20070185014A1 (en) * | 2006-02-09 | 2007-08-09 | The Schepens Eye Research Institute, Inc. | Methods and compositions for modulating conjunctival goblet cells |
-
2007
- 2007-02-23 EP EP07736536A patent/EP1987133A2/en not_active Withdrawn
- 2007-02-23 NZ NZ570746A patent/NZ570746A/en not_active IP Right Cessation
- 2007-02-23 US US11/710,623 patent/US8338175B2/en not_active Expired - Fee Related
- 2007-02-23 WO PCT/IN2007/000075 patent/WO2007099556A2/en active Application Filing
- 2007-02-23 JP JP2008555952A patent/JP2009527250A/en active Pending
- 2007-02-23 CA CA002642947A patent/CA2642947A1/en not_active Abandoned
- 2007-02-23 RU RU2008137975/13A patent/RU2008137975A/en not_active Application Discontinuation
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- 2007-02-23 CN CNA2007800148149A patent/CN101432031A/en active Pending
-
2008
- 2008-08-21 IL IL193597A patent/IL193597A0/en unknown
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20040009590A1 (en) * | 2002-03-29 | 2004-01-15 | Donald Tan | Method for growth of human conjunctival tissue equivalents for research, clinical ocular surface transplantation and tissue engineering |
US20050186672A1 (en) * | 2004-01-27 | 2005-08-25 | Reliance Life Sciences Pvt. Ltd. | Tissue system with undifferentiated stem cells derived from corneal limbus |
Non-Patent Citations (7)
Title |
---|
ANG L P K ET AL: "The use of human serum in supporting the in vitro and in vivo proliferation of human conjunctival epithelial cells" BRITISH JOURNAL OF OPHTHALMOLOGY, vol. 89, no. 6, June 2005 (2005-06), pages 748-752, XP009088079 ISSN: 0007-1161 * |
ANG LEONARD P K ET AL: "The in vitro and in vivo proliferative capacity of serum-free cultivated human conjunctival epithelial cells." CURRENT EYE RESEARCH MAY 2004, vol. 28, no. 5, May 2004 (2004-05), pages 307-317, XP009088081 ISSN: 0271-3683 * |
KINOSHITA SHIGERU ET AL: "Transplantable cultivated mucosal epithelial sheet for ocular surface reconstruction." EXPERIMENTAL EYE RESEARCH, vol. 78, no. 3, March 2004 (2004-03), pages 483-491, XP002446322 ISSN: 0014-4835 * |
KOIZUMI N ET AL: "Cultivated corneal epithelial stem cell transplantation in ocular surface disorders" OPHTHALMOLOGY, J. B. LIPPINCOTT CO., PHILADELPHIA, PA, US, vol. 108, no. 9, 2001, pages 1569-1574, XP002988711 ISSN: 0161-6420 * |
NAKAMURA TAKAHIRO ET AL: "Successful primary culture and autologous transplantation of corneal limbal epithelial cells from minimal biopsy for unilateral severe ocular surface disease" ACTA OPHTHALMOLOGICA SCANDINAVICA, vol. 82, no. 4, August 2004 (2004-08), pages 468-471, XP002446321 ISSN: 1395-3907 * |
TAN D T ET AL: "RECONSTRUCTION OF THE OCULAR SURFACE BY TRANSPLANTATION OF A SERUM-FREE DERIVED CULTIVATED CONJUNCTIVAL EPITHELIAL EQUIVALENT" TRANSPLANTATION, WILLIAMS AND WILKINS, BALTIMORE, MD, US, vol. 77, no. 11, 15 June 2004 (2004-06-15), pages 1729-1734, XP009045642 ISSN: 0041-1337 cited in the application * |
TSAI R J-F ET AL: "Reconstruction of damaged corneas by transplantation of autologous limbal epithelial cells" NEW ENGLAND JOURNAL OF MEDICINE, THE, MASSACHUSETTS MEDICAL SOCIETY, WALTHAM, MA, US, vol. 43, no. 2, 13 July 2000 (2000-07-13), pages 86-93, XP002968878 ISSN: 0028-4793 * |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2008131973A2 (en) * | 2007-04-26 | 2008-11-06 | Medinnova As | Transplant storage |
WO2008131973A3 (en) * | 2007-04-26 | 2010-05-14 | Medinnova As | Transplant storage |
WO2010049181A1 (en) * | 2008-10-29 | 2010-05-06 | Oslo University Hospital | Storage of conjuctival cells |
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RU2008137975A (en) | 2010-03-27 |
AU2007220122A1 (en) | 2007-09-07 |
NZ570746A (en) | 2012-02-24 |
CA2642947A1 (en) | 2007-09-07 |
IL193597A0 (en) | 2011-08-01 |
CN101432031A (en) | 2009-05-13 |
WO2007099556A3 (en) | 2007-10-18 |
EP1987133A2 (en) | 2008-11-05 |
AU2007220122B2 (en) | 2014-01-16 |
KR20080097226A (en) | 2008-11-04 |
US20070218039A1 (en) | 2007-09-20 |
US8338175B2 (en) | 2012-12-25 |
JP2009527250A (en) | 2009-07-30 |
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