WO2007076464A3 - Thermostable dna polymerase from thermus filiformis - Google Patents

Thermostable dna polymerase from thermus filiformis Download PDF

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Publication number
WO2007076464A3
WO2007076464A3 PCT/US2006/062534 US2006062534W WO2007076464A3 WO 2007076464 A3 WO2007076464 A3 WO 2007076464A3 US 2006062534 W US2006062534 W US 2006062534W WO 2007076464 A3 WO2007076464 A3 WO 2007076464A3
Authority
WO
WIPO (PCT)
Prior art keywords
thermus filiformis
dna polymerase
thermostable dna
polymerase
dna
Prior art date
Application number
PCT/US2006/062534
Other languages
French (fr)
Other versions
WO2007076464A2 (en
Inventor
Guolu Hu
Scott Hamilton
Carl W Fuller
Original Assignee
Ge Healthcare Bio Sciences
Guolu Hu
Scott Hamilton
Carl W Fuller
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ge Healthcare Bio Sciences, Guolu Hu, Scott Hamilton, Carl W Fuller filed Critical Ge Healthcare Bio Sciences
Publication of WO2007076464A2 publication Critical patent/WO2007076464A2/en
Publication of WO2007076464A3 publication Critical patent/WO2007076464A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Abstract

Disclosed is a thermostable DNA polymerase from Thermus filiformis. This enzyme is useful for DNA sequencing, reverse transcription and polymerase chain reaction. Included within the scope of the present invention are various mutants (deletion and substitution) that retain thermostability and the ability to replicate DNA with substantially the same efficiency as the native Thermus filiformis polymerase.
PCT/US2006/062534 2005-12-22 2006-12-22 Thermostable dna polymerase from thermus filiformis WO2007076464A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US75314705P 2005-12-22 2005-12-22
US60/753,147 2005-12-22

Publications (2)

Publication Number Publication Date
WO2007076464A2 WO2007076464A2 (en) 2007-07-05
WO2007076464A3 true WO2007076464A3 (en) 2007-08-16

Family

ID=38093293

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/062534 WO2007076464A2 (en) 2005-12-22 2006-12-22 Thermostable dna polymerase from thermus filiformis

Country Status (1)

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WO (1) WO2007076464A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9587264B2 (en) 2001-09-14 2017-03-07 Applied Biosystems, Llc Thermus scotoductus nucleic acid polymerases

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU743025B2 (en) 1997-03-12 2002-01-17 Applera Corporation DNA polymerases having improved labeled nucleotide incorporation properties
AU2002346498A1 (en) 2001-11-30 2003-06-17 Applera Corporation Thermus brockianus nucleic acid polymerases

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001014568A1 (en) * 1999-08-21 2001-03-01 Amersham Pharmacia Biotech Inc. Taq dna polymerases having an amino acid substitution at e681 and homologs thereof exhibiting improved salt tolerance
WO2003004632A2 (en) * 2001-07-06 2003-01-16 Amersham Biosciences Corp Novel dna polymerases having amino acid substitutions and homologs thereof
WO2003066804A2 (en) * 2001-09-14 2003-08-14 Applera Corporation Thermus scotoductus nucleic acid polymerases

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001014568A1 (en) * 1999-08-21 2001-03-01 Amersham Pharmacia Biotech Inc. Taq dna polymerases having an amino acid substitution at e681 and homologs thereof exhibiting improved salt tolerance
WO2003004632A2 (en) * 2001-07-06 2003-01-16 Amersham Biosciences Corp Novel dna polymerases having amino acid substitutions and homologs thereof
WO2003066804A2 (en) * 2001-09-14 2003-08-14 Applera Corporation Thermus scotoductus nucleic acid polymerases

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
GUTMAN P D ET AL: "CONSERVED SITES IN THE 5'-3' EXONUCLEASE DOMAIN OF ESCHERICHIA COLI DNA POLYMERASE", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 21, no. 18, September 1993 (1993-09-01), pages 4406 - 4407, XP000857830, ISSN: 0305-1048 *
JUNG S E ET AL: "CLONING AND ANALYSIS OF THE DNA POLYMERASE-ENCODING GENE FROM THERMUS FILIFORMIS", MOLECULES AND CELLS, SEOUL, KR, vol. 7, no. 6, 31 December 1997 (1997-12-31), pages 769 - 776, XP008053927, ISSN: 1016-8478 *
SHANDILYA H ET AL: "Thermophilic bacterial DNA polymerases with reverse-transcriptase activity", EXTREMOPHILES, SPRINGER VERLAG, TOKYO,, JP, vol. 8, no. 3, June 2004 (2004-06-01), pages 243 - 251, XP002340627, ISSN: 1431-0651 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9587264B2 (en) 2001-09-14 2017-03-07 Applied Biosystems, Llc Thermus scotoductus nucleic acid polymerases

Also Published As

Publication number Publication date
WO2007076464A2 (en) 2007-07-05

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