WO2007057057A1 - Pvdf membranes - Google Patents
Pvdf membranes Download PDFInfo
- Publication number
- WO2007057057A1 WO2007057057A1 PCT/EP2006/004356 EP2006004356W WO2007057057A1 WO 2007057057 A1 WO2007057057 A1 WO 2007057057A1 EP 2006004356 W EP2006004356 W EP 2006004356W WO 2007057057 A1 WO2007057057 A1 WO 2007057057A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- membrane
- antibody
- protein
- plates
- pvdf
- Prior art date
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D69/00—Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or properties; Manufacturing processes specially adapted therefor
- B01D69/02—Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or properties; Manufacturing processes specially adapted therefor characterised by their properties
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D67/00—Processes specially adapted for manufacturing semi-permeable membranes for separation processes or apparatus
- B01D67/0081—After-treatment of organic or inorganic membranes
- B01D67/0088—Physical treatment with compounds, e.g. swelling, coating or impregnation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D71/00—Semi-permeable membranes for separation processes or apparatus characterised by the material; Manufacturing processes specially adapted therefor
- B01D71/06—Organic material
- B01D71/30—Polyalkenyl halides
- B01D71/32—Polyalkenyl halides containing fluorine atoms
- B01D71/34—Polyvinylidene fluoride
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2323/00—Details relating to membrane preparation
- B01D2323/02—Hydrophilization
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2325/00—Details relating to properties of membranes
- B01D2325/36—Hydrophilic membranes
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T428/00—Stock material or miscellaneous articles
- Y10T428/31504—Composite [nonstructural laminate]
- Y10T428/3154—Of fluorinated addition polymer from unsaturated monomers
Definitions
- the invention relates to a method of treating PVDF membranes to eliminate the need for preactivation.
- the treated membranes have utility in ELISpot and other assays.
- PVDF Polyvinyl difluoride
- the membrane is treated with ethanol for a short period, followed by several rounds of washing before addition of the substance to be coated. It is essential that the membrane is not allowed to dry between the activation and the coating as it then resumes its hydrophobic low-binding properties.
- the activation procedure needs to be performed in a strictly defined manner, particularly when the membrane is used for cellular assays, since "overtreatment” (i.e. too much ethanol or too long a treatment duration) has been shown to have a deleterious effect and lead to suboptimal results.
- the present inventors have developed a method of modifying a PVDF membrane in a way that obviates the need for the user to preactivate before coating. Modifying the membrane is this way avoids the risk of "overtreatment".
- the use of modified membranes also represents a significant simplification in the preparation of assay plates because the coating substance can be added directly to the plate, without the need for prior activation. Accordingly, the present invention provides: a method of treating a polyvinyl difluoride (PVDF) membrane comprising:
- Figure 1 shows typical IFN- ⁇ ELISpot results using different conventional coating procedures.
- Figure 2 shows typical IFN- ⁇ ELISpot results using different coating procedures.
- A precoated control plates;
- B plates wetted with ethanol and dried with glycerol;
- C plates wetted with ethanol and dried with protein (2 ⁇ g irrelevant antibody); and
- D plates treated with a mixture of ethanol and trehalose and dried. In control wells without antigen the number of spots was always less than 5.
- Figure 3 shows typical IL-10 ELISpot results using different coating procedures.
- the number of spots in control well without antigen was below 5 in each plate.
- Figure 4 shows IL- 12 ELISpot results using different coating procedures.
- the number of spots in control wells without antigen was below 5 in all plates.
- Figure 5 shows IL- 13 ELISpot results using different coating procedures.
- (row 3) plates treated with a mixture of ethanol and trehalose and dried. The number of spots in each plate is indicated under each picture (n 4).
- the present invention is based on the finding that polyvinyl difluoride (PVDF) membranes can be "activated” in a permanent way if they, after or during activation with ethanol, are saturated with substances ("wetting agents") that make the membrane more hydrophilic. This way the membrane can be dried and stored long-term while still retaining its high binding properties.
- PVDF polyvinyl difluoride
- the method of the invention may also be applied to other hydrophobic membranes suitable for use in the ELISpot assay.
- the invention provides a method of treating, or activating, a PVDF membrane comprising:
- Step (b) drying said membrane.
- Step (a) may comprise contacting said membrane with a mixture of the alcohol and the wetting agent.
- the membrane may be first contacted with alcohol and then with the wetting agent.
- the membrane may be washed to remove the alcohol prior to adding the wetting agent.
- the alcohol is preferably methanol or ethanol but other alcohols such as propanol or isopropanol may be used.
- the wetting agent is any substance that makes the PVDF membrane more hydrophilic.
- the wetting agent is a hydrophilic or partly hydrophilic compound, i.e. a compound which is both hydrophilic and hydrophobic.
- the wetting agent is preferably non-toxic.
- the wetting agent typically has no effect on cell activation or cell behaviour.
- the wetting agent is generally endotoxin free.
- Suitable wetting agents include phosphate buffered saline (PBS), carbonate salts, carbohydrates, proteins and amino acids. In one embodiment, two or more, such as 3, 4, 5 or 6, different wetting agents may be used in combination.
- two or more different carbohydrates such as two or more of the sugars described herein, two or more different carbonate salts, two or more different proteins or two or more different amino acids may be used as wetting agents.
- two or more different types of wetting agent may be used in combination.
- a carbohydrate and a protein, amino acid and/or carbonate salt may be used, optionally in PBS
- a protein, amino acid and/or carbonate salt may be used, optionally in PBS or an amino acid and a carbonate salt may be used, optionally in PBS.
- the carbohydrate is typically a sugar.
- suitable sugars include lactose, glucose, sucrose, fructose, mannose, inositol, glucosamine, arabinose, xylose and trehalose.
- the protein may be any protein that does not bind to substances, such as cytokines, that the membrane may subsequently be used to detect.
- the protein may, for example, be an antibody which does not interact with any of the molecules of interest in the assay for which the membrane is intended. Suitable proteins are typically from 5 to 5000 amino acids in length. The protein is typically in purified form. The protein is generally one which is stable over time to allow long term storage of the plates.
- amino acid may be used as the wetting agent.
- the amino acid may be alanine, arginine, asparagine, aspartate, cysteine, glutamine, glutamate, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine or valine or any combination thereof.
- Glycine is a preferred amino acid.
- the time for which the membrane is contacted with the alcohol and wetting agent is not critical.
- the membrane is typically contacted with a mixture of alcohol and the wetting agent for from about 30 seconds to about 10 minutes.
- the membrane may be subjected to drying immediately after the mixture of alcohol and wetting agent is added to the membrane.
- the membrane is typically contacted with alcohol for from about 30 seconds to about 10 minutes followed by contacting the membrane with the wetting agent for from about 30 seconds to about 10 minutes.
- the wetting agent may be added immediately after the alcohol.
- the membrane may be subjected to drying immediately after the wetting agent is added.
- 70% alcohol for example 70% ethanol or 70% methanol is used in a method of the invention.
- the alcohol may also be used at other concentrations, for example from 30% to 90%.
- Suitable concentrations of the different wetting agents are as follows.
- Carbohydrates may be used at a concentration of from about 0.05% to about 5%, for example from about 0.1% to about 4% or about 0.2% to about 3%.
- the carbohydrate may be present in PBS.
- Glycerol may be used at a concentration of from about 0.1% to about 10%, for example at about 1%, 2% or 5%.
- PBS may be used at various different dilutions, such as from about 0.5 to about 10 times the normal dilution, such as at the normal dilution or twice the normal dilution.
- the normal dilution of PBS is 2OmM phosphate and 5OmM NaCl.
- amino acid or combination of amino acids, may be used at a concentration of from 0.05% to 5%, for example at 0.5%, 1% or 2%.
- a protein, such as an antibody may be used at a concentration of from about O.l ⁇ gml '1 to about lO ⁇ gml "1 , such as at about l ⁇ gmr' ⁇ gml "1 or 5 ⁇ gmr ⁇
- the membrane is typically dried in the presence of the wetting agent, and optionally the alcohol, under sterile conditions.
- the membrane may be dried in a dry climate room under a sterile flow.
- Sterile conditions may be applied by keeping the plates under a UV light.
- drying may be carried out under non-sterile conditions and the plates may be sterilized by irradiation after drying. Drying may also be carried out under a vacuum.
- the plates may be heated to aid the drying process.
- the drying time is not critical and depends on the volume of ethanol/wetting agent added and the drying conditions.
- the drying step is typically carried out in the absence of a stabilising agent, particularly in the case where the wetting agent is a protein.
- the PVDF membrane may be in any format.
- a sheet of PVDF may be treated by a method of the invention.
- the PVDF membrane may be in a format suitable for a particular assay.
- the PVDF membrane may be in a 96 well ELISpot plate. In a 96 well ELISpot plate the PVDF may be in every well, a strip of wells or a single well.
- the present invention also provides a method of treating a PVDF membrane which further comprises:
- the protein may be any protein that is capable of specifically binding to a substance to be detected in an assay.
- the protein may, for example, be an antibody or fragment thereof.
- the antibody may be any antibody or binding protein that is useful in an immunoassay.
- the antibody may be one specific for a cytokine such as IFN- ⁇ , IL-IO, IL- 12 or IL- 13.
- the invention also provides a PVDF membrane obtainable by a method of the invention.
- the PVDF membrane is typically provided in a dried form.
- the membrane of the invention does not require activation prior to its subsequent use, i.e. coating of the membrane with a binding protein.
- the membrane of the invention may be coated by contacting it directly with an antibody or other binding protein in solution.
- the invention also provides a method of coating a PVDF membrane of the invention with a protein, said method comprising:
- the present invention provides the use of a PVDF membrane according to the invention in an immunological assay.
- immunological assays include ELISpot and ELISA assays.
- the PVDF membranes of the invention may be provided together with instructions for coating the membrane with a protein, such as an antibody, and/or instructions for carrying out an immunological assay.
- the membranes may also be provided together with any components of an immunological assay, such as an antibody.
- the invention also provides an immunoassay kit comprising a membrane of the invention together with one or more of an antibody, a buffer, a cell culture medium, an enzyme-conjugated antibody, a streptavidin-enzyme conjugate, an enzyme substrate and/or assay instructions.
- the antibody may be for binding to the membrane as a capture antibody or may be an antibody for detecting a captured reagent.
- the detection antibody may be biotinylated. Where the kit comprises a biotinylated detection antibody it typically also comprises a streptavidin-enzyme conjugate. The following Examples illustrate the invention.
- IFN- ⁇ ELISpot assays were carried out using different conventional coating procedures.
- ELISpot plates Commercially available precoated ELISpot plates (Mabtech, Sweden) were used as a precoated control plate.
- the EFN- ⁇ antibodies have been coated onto ethanol activated membranes followed by drying in the presence of stabilising agents. This procedure has previously been shown to produce plates that are optionally sensitive and highly stable when stored long term under various conditions. Also, although the membrane is activated by ethanol, the treatment conditions are chosen such that the negative effects of ethanol treatment are not observed.
- PVDF ELISpot plate was prewetted with lOO ⁇ l 70% ethano I/well for 2 minutes prior to coating with anti-IFN- ⁇ antibody (IDlK) at 1.5 ⁇ g per well
- IDlK anti-IFN- ⁇ antibody
- ELISpot plates were incubated with 10 x 10 4 peripheral blood mononuclear cells (PBMC) ⁇ vell overnight in the presence of antigenic peptides (CEF peptide pool).
- PBMC peripheral blood mononuclear cells
- CEF peptide pool antigenic peptides
- SA-ALP streptavidin-alkaline phosphatase conjugate
- BC1P/NBT substrate
- Spots were counted using an automated ELISpot reader from AID, Germany.
- Figure 1 shows typical results from an ELISpot with a correctly activated well (A) and an'Overtreated" well (B). As can be seen, overtreatment leads to significantly reduced number of spots which are also less distinct and thereby more difficult to evaluate.
- Millipore To control the adverse effect of the ethanol treatment, the protocol currently supplied by the current leading manufacturer of ELISpot plates, Millipore requires a very short treatment ( ⁇ 1 min) with a small volume of ethanol or methanol (15 ⁇ l/well in a 96 well plate). However, these conditions are not always easy to follow in practice and may not be sufficient to avoid the negative effect.
- Example 1 Treatment of PVDF membranes with ethanol and a wetting agent using 2-step and 1-step procedures
- the plates were activated in a conventional way with 70% ethanol (50 ⁇ l/well for 2 min) followed by washing with distilled water. 25- 50 ⁇ l/well wetting agent was thereafter added and the plates were dried under sterile conditions.
- the plates were subjected to a mixture of ethanol and wetting agent (0.2% trehalose). 50 ⁇ l or more of this mixture was added into each well followed by drying under sterile conditions.
- wetting agent 0.2% trehalose
- 50 ⁇ l or more of this mixture was added into each well followed by drying under sterile conditions.
- Several different compounds were tested to achieve the desired wetting effect. These included salts such as PBS (phosphate buffered saline) and carbonate salts, sugars such as lactose, glucose and trehalose as well as proteins, glycerol and the amino acid glycine.
- precoated control After drying, the plates were packed with dry-packs and kept at room temperature until used.
- IFN- ⁇ ELISpot arrays were performed on the variously treated plates as follows. In the IFN- ⁇ test ELISpot assays, the 2-step procedure was carried out using
- PBMC peripheral blood mononuclear cells
- IFN- ⁇ ELISpot assays PBMC (1O x 10 4 /well) were incubated with IFN- ⁇ -coated plates in the presence of stimulating antigenic peptides (CEF peptide pool). After incubation the cells were removed and the plates developed and analysed as described above. Typical results are shown in Figure 2.
- IL-IO, IL- 12 and IL- 13 ELISpot test assays were carried out using untreated plates (no prewetting prior antibody coating), plates treated with ethanol by a conventional method (freshly prewetted with lOO ⁇ l 70% ethano I/well for 2 minutes prior to coating with antibody) and plates treated with a mixture of ethanol and trehalose according to the 1-step procedure described in Example 1 prior to coating with 15 ⁇ gml " ' of the monoclonal antibodies anti-IL-10 (9D7), anti-IL-12 (IL-12-1) and anti-IL-13 (IL-13-1), respectively.
- PBMC 125 x 10 3 /well
- PBMC 125 x 10 3 /well
- PPD purified protein derivative
- PHA phytohemagglutinin
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Inorganic Chemistry (AREA)
- Manufacturing & Machinery (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
Claims
Priority Applications (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AU2006314838A AU2006314838B2 (en) | 2005-05-09 | 2006-05-09 | PVDF membranes |
JP2008510489A JP5199069B2 (en) | 2005-05-09 | 2006-05-09 | PVDF membrane |
EP20060840907 EP1891442B1 (en) | 2005-05-09 | 2006-05-09 | Pvdf membranes |
CA 2608277 CA2608277C (en) | 2005-05-09 | 2006-05-09 | Pvdf membranes |
US11/920,033 US20090068688A1 (en) | 2005-05-09 | 2006-05-09 | PVDF membranes |
US13/308,484 US8859219B2 (en) | 2005-05-09 | 2011-11-30 | PVDF membranes |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB0509422A GB0509422D0 (en) | 2005-05-09 | 2005-05-09 | Membranes |
GB0509422.2 | 2005-05-09 |
Related Child Applications (3)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US11/920,033 A-371-Of-International US20090068688A1 (en) | 2005-05-09 | 2006-05-09 | PVDF membranes |
US12/363,948 Continuation US20090218037A1 (en) | 2003-03-18 | 2009-02-02 | Piping |
US13/308,484 Continuation US8859219B2 (en) | 2005-05-09 | 2011-11-30 | PVDF membranes |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2007057057A1 true WO2007057057A1 (en) | 2007-05-24 |
Family
ID=34685300
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/EP2006/004356 WO2007057057A1 (en) | 2005-05-09 | 2006-05-09 | Pvdf membranes |
Country Status (7)
Country | Link |
---|---|
US (2) | US20090068688A1 (en) |
EP (1) | EP1891442B1 (en) |
JP (1) | JP5199069B2 (en) |
AU (1) | AU2006314838B2 (en) |
CA (1) | CA2608277C (en) |
GB (1) | GB0509422D0 (en) |
WO (1) | WO2007057057A1 (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101439268B (en) * | 2008-12-09 | 2011-06-29 | 南京龙源环保有限公司 | Method for preparing high-intensity high-throughput polyvinylidene fluoride hollow fiber membrane |
US8859219B2 (en) | 2005-05-09 | 2014-10-14 | Mabtech Ab | PVDF membranes |
CN106334463A (en) * | 2016-10-17 | 2017-01-18 | 东华大学 | Anti-pollution polyvinylidene fluoride hollow-fiber membrane and preparation method thereof |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE102009022513A1 (en) * | 2009-05-25 | 2010-12-02 | Qiagen Gmbh | Method of protecting membranes |
RU2524631C2 (en) | 2010-01-07 | 2014-07-27 | Колгейт-Палмолив Компани | Changing of colour of chalcone-containing preparative forms for oral cavity care |
SG10201609398YA (en) * | 2012-05-11 | 2016-12-29 | Agency Science Tech & Res | A Method For Treating A Porous Membrane And Uses Thereof |
US11219869B2 (en) * | 2017-04-27 | 2022-01-11 | Unm Rainforest Innovations | Enzymatically active high-flux selectively gas-permeable membranes for enhanced oil recovery and carbon capture |
CN109224872B (en) * | 2018-09-04 | 2021-02-12 | 南京工业大学 | Amino acid ionic liquid modified hydrophilic nanofiltration membrane and preparation method thereof |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4340482A (en) * | 1978-02-21 | 1982-07-20 | Millipore Corporation | Process for grafting amino acid molecules onto preformed polymer surfaces and products prepared thereby |
EP0353460A2 (en) * | 1988-06-28 | 1990-02-07 | Millipore Corporation | Membranes for solid phase protein sequencing |
US5827650A (en) * | 1991-12-23 | 1998-10-27 | Tropix, Inc. | Membrane for chemiluminescent blotting applications |
WO2001005492A1 (en) * | 1999-07-16 | 2001-01-25 | Baxter International Inc | Polyvinylidene difluoride membranes and methods for making such membranes |
DE10225501A1 (en) * | 2002-06-10 | 2003-12-24 | Eppendorf Ag | Process for immobilizing proteins contained in a liquid sample with subsequent drying of the immobilized proteins, and solid-phase substrates which can be used in the process |
US20040214180A1 (en) * | 2003-04-28 | 2004-10-28 | Hironori Kobayashi | Method and kit for determining quantity of protein |
Family Cites Families (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5019260A (en) * | 1986-12-23 | 1991-05-28 | Pall Corporation | Filtration media with low protein adsorbability |
JPH01107812A (en) * | 1987-10-19 | 1989-04-25 | Fuji Photo Film Co Ltd | Method for making porous membrane hydrophilic |
DK438689D0 (en) | 1989-09-05 | 1989-09-05 | Danisco | HYDROPHIL MEMBRANE FOR USE BY ULTRAFILTRATION OR MICROFILTRATION AND PROCEDURES FOR PRODUCING THEREOF |
US5476590A (en) * | 1995-03-14 | 1995-12-19 | Sartorius Ag | Hydrophilic polyvinylidene fluoride membranes |
US6410252B1 (en) * | 1995-12-22 | 2002-06-25 | Case Western Reserve University | Methods for measuring T cell cytokines |
EP1419387B1 (en) | 2001-08-20 | 2012-01-04 | Proteome Systems Ltd. | Diagnostic testing process |
US6734386B1 (en) * | 2001-09-24 | 2004-05-11 | Meissner Filtration Products, Inc. | Method for rendering PVDF membrane hydrophilic |
US20040063220A1 (en) * | 2002-02-27 | 2004-04-01 | Lebrun Stewart J. | Substrate chemistry for protein immobilization on a rigid support |
AU2003903507A0 (en) * | 2003-07-08 | 2003-07-24 | U. S. Filter Wastewater Group, Inc. | Membrane post-treatment |
US7338692B2 (en) * | 2003-09-12 | 2008-03-04 | 3M Innovative Properties Company | Microporous PVDF films |
US7517581B2 (en) * | 2003-09-26 | 2009-04-14 | Parker-Hannifin Corporation | Semipermeable hydrophilic membrane |
GB0509422D0 (en) | 2005-05-09 | 2005-06-15 | Mabtech Ab | Membranes |
US8132676B2 (en) * | 2008-08-18 | 2012-03-13 | Emd Millipore Corporation | Hydrophilic, high protein binding, low fluorescence, western blotting membrane |
JP2012527738A (en) * | 2009-05-20 | 2012-11-08 | ポーラス パワー テクノロジーズ,エルエルシー | Microporous membrane treatment and adhesives |
-
2005
- 2005-05-09 GB GB0509422A patent/GB0509422D0/en not_active Ceased
-
2006
- 2006-05-09 CA CA 2608277 patent/CA2608277C/en not_active Expired - Fee Related
- 2006-05-09 JP JP2008510489A patent/JP5199069B2/en not_active Expired - Fee Related
- 2006-05-09 AU AU2006314838A patent/AU2006314838B2/en not_active Ceased
- 2006-05-09 US US11/920,033 patent/US20090068688A1/en not_active Abandoned
- 2006-05-09 EP EP20060840907 patent/EP1891442B1/en not_active Not-in-force
- 2006-05-09 WO PCT/EP2006/004356 patent/WO2007057057A1/en active Application Filing
-
2011
- 2011-11-30 US US13/308,484 patent/US8859219B2/en not_active Expired - Fee Related
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4340482A (en) * | 1978-02-21 | 1982-07-20 | Millipore Corporation | Process for grafting amino acid molecules onto preformed polymer surfaces and products prepared thereby |
EP0353460A2 (en) * | 1988-06-28 | 1990-02-07 | Millipore Corporation | Membranes for solid phase protein sequencing |
US5827650A (en) * | 1991-12-23 | 1998-10-27 | Tropix, Inc. | Membrane for chemiluminescent blotting applications |
WO2001005492A1 (en) * | 1999-07-16 | 2001-01-25 | Baxter International Inc | Polyvinylidene difluoride membranes and methods for making such membranes |
DE10225501A1 (en) * | 2002-06-10 | 2003-12-24 | Eppendorf Ag | Process for immobilizing proteins contained in a liquid sample with subsequent drying of the immobilized proteins, and solid-phase substrates which can be used in the process |
US20040214180A1 (en) * | 2003-04-28 | 2004-10-28 | Hironori Kobayashi | Method and kit for determining quantity of protein |
Non-Patent Citations (2)
Title |
---|
CHABRAOUI F ET AL: "DOT-BLOT IMMUNODETECTION OF ANTIBODIES AGAINST G MI AND OTHER GANGLIOSIDES ON PVDF-P MEMBRANES", JOURNAL OF IMMUNOLOGICAL METHODS, ELSEVIER SCIENCE PUBLISHERS B.V.,AMSTERDAM, NL, vol. 165, no. 2, 1993, pages 225 - 230, XP002065880, ISSN: 0022-1759 * |
LIN YING ET AL: "Detection of multiple cytokines by protein arrays from cell lysate and tissue lysate.", CLINICAL CHEMISTRY AND LABORATORY MEDICINE, vol. 41, no. 2, February 2003 (2003-02-01), pages 139 - 145, XP009070355, ISSN: 1434-6621 * |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8859219B2 (en) | 2005-05-09 | 2014-10-14 | Mabtech Ab | PVDF membranes |
CN101439268B (en) * | 2008-12-09 | 2011-06-29 | 南京龙源环保有限公司 | Method for preparing high-intensity high-throughput polyvinylidene fluoride hollow fiber membrane |
CN106334463A (en) * | 2016-10-17 | 2017-01-18 | 东华大学 | Anti-pollution polyvinylidene fluoride hollow-fiber membrane and preparation method thereof |
Also Published As
Publication number | Publication date |
---|---|
EP1891442A1 (en) | 2008-02-27 |
EP1891442B1 (en) | 2013-07-17 |
JP2008541073A (en) | 2008-11-20 |
CA2608277C (en) | 2015-03-17 |
US20090068688A1 (en) | 2009-03-12 |
US8859219B2 (en) | 2014-10-14 |
GB0509422D0 (en) | 2005-06-15 |
AU2006314838B2 (en) | 2012-03-29 |
AU2006314838A1 (en) | 2007-05-24 |
CA2608277A1 (en) | 2007-05-24 |
JP5199069B2 (en) | 2013-05-15 |
US20120142034A1 (en) | 2012-06-07 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US8859219B2 (en) | PVDF membranes | |
EP0221046B1 (en) | Modified polymeric surfaces and process for preparing same | |
US7771955B2 (en) | Affinity membrane for capture of a target biomolecule and formation thereof by site-directed immobilization of a capture biomolecule | |
US5071909A (en) | Immobilization of proteins and peptides on insoluble supports | |
US4923680A (en) | Test device containing an immunoassay filter with a flow-delaying polymer | |
US20070224688A1 (en) | Peptide or protein-capturing surfaces for high throughput MALDI mass spectrometry | |
ES2397680T3 (en) | Wester transfer membrane, hydrophilic, high protein binding, low fluorescence | |
CN109142697B (en) | Biological sample quantitative collector and application thereof | |
JP2016530217A5 (en) | ||
Dong et al. | Limited proteolysis in porous membrane reactors containing immobilized trypsin | |
CA2578202C (en) | Fractionation apparatus | |
JPS61145447A (en) | Immobilized enzyme membrane | |
JPS61152700A (en) | Membrane supporting immobilized protein | |
WO1987001120A1 (en) | Method of attaching biological substances to solid substrates | |
JP4432411B2 (en) | Laser desorption ionization mass spectrometry | |
JPS6161068B2 (en) | ||
AU605746B2 (en) | Method of attaching biological substances to solid substrates | |
JP2002243743A (en) | Method of detecting tested substance | |
Ahmed et al. | Orientational Control of Immobilized Biomolecules on a Membrane Surface for Specific Protein Capture | |
JP3874520B2 (en) | Carrier for immunoassay and method for preparing solid phase for immunoassay using the same | |
CN114660283A (en) | Immunoassay plate type chip based on electrical acceleration and preparation method thereof | |
WO2004113924A2 (en) | Method and system for generating and capturing peptides on a target surface | |
JPWO2006109685A1 (en) | How to collect plasma or serum | |
JPS59135360A (en) | Enzyme electrode |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
121 | Ep: the epo has been informed by wipo that ep was designated in this application | ||
WWE | Wipo information: entry into national phase |
Ref document number: 2608277 Country of ref document: CA Ref document number: 2008510489 Country of ref document: JP |
|
NENP | Non-entry into the national phase |
Ref country code: DE |
|
WWW | Wipo information: withdrawn in national office |
Country of ref document: DE |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2006840907 Country of ref document: EP |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2006314838 Country of ref document: AU |
|
ENP | Entry into the national phase |
Ref document number: 2006314838 Country of ref document: AU Date of ref document: 20060509 Kind code of ref document: A |
|
WWP | Wipo information: published in national office |
Ref document number: 2006314838 Country of ref document: AU |
|
WWE | Wipo information: entry into national phase |
Ref document number: 11920033 Country of ref document: US |
|
WWP | Wipo information: published in national office |
Ref document number: 2006840907 Country of ref document: EP |