WO2007042286A1 - Egfr dependent modulation of chemokine expression and influence on therapy and diagnosis of tumors and side effects thereof - Google Patents
Egfr dependent modulation of chemokine expression and influence on therapy and diagnosis of tumors and side effects thereof Download PDFInfo
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- G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
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- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6863—Cytokines, i.e. immune system proteins modifying a biological response such as cell growth proliferation or differentiation, e.g. TNF, CNF, GM-CSF, lymphotoxin, MIF or their receptors
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- G01N2800/52—Predicting or monitoring the response to treatment, e.g. for selection of therapy based on assay results in personalised medicine; Prognosis
Definitions
- the invention relates to diagnosis and therapy of tumors utilizing the epidermal growth factor (EGFR) by means of chemical inhibitors or monoclonal antibodies.
- EGFR epidermal growth factor
- the invention relates also to skin irritations, preferably skin rash, in conjunction and associated with the treatment of tumors that utilize EGF receptor with anticancer agents.
- the invention is also directed to methods of predicting the efficiency of a tumor therapy / tumor response in a patient based on the treatment with EGFR inhibitors, especially anti-EGFR antibodies.
- the invention further relates to a method of determining the optimum dose of an anti-cancer agent in EGFR related tumor therapy.
- the invention further relates to methods of early stage monitoring of the efficiency of EGFR related cancer therapy by means of EGFR inhibitors, and of the likelihood of occurrence of skin rash as side effect disease in conjunction with said therapy.
- the invention is directed to the use of chemokines, which are up- or down-regulated during cancer treatment by means of an anti-cancer agent, as a diagnostic marker or as leads for the identification of noval targets for tumor therapeutics.
- EGFR encoded by the erbB1 gene
- EGFR has been causally implicated in human malignancy.
- increased expression of EGFR has been observed in breast, bladder, lung, head, neck and stomach cancer as well as glioblastomas.
- Increased EGFR receptor expression is often associated with increased production of the EGFR ligand, transforming growth factor alpha (TGF-a), by the same tumor cells resulting in receptor activation by an autocrine stimulatory pathway (Baselga and Mendelsohn, Pha ⁇ nac. Ther. 64:127 (1994)).
- TGF-a transforming growth factor alpha
- the EGF receptor is a transmembrane glycoprotein which has a molecular weight of 170.000, and is found on many epithelial cell types.
- EGFR ligands which upon receptor binding protect tumor cells from apoptosis stimulate cell proliferation and tumor cell invasiveness. These growth factors do not bind to HER2, HER3 and HER4 the other three members of the EGFR family which can engage with the EGFR in forming heterodimers (Riese and Stern. ⁇ /oassays 20: 41-48 (1998); Kochupurakkal J Biol. Chem. 280:8503- 8512 (2005))
- the HER receptor network might integrate not only its own inputs but also heterologous signals, including hormones, lymphokines, neurotransmitters and stress inducers.
- tyrosine kinase antagonist/inhibitor refers according to this invention to natural or synthetic agents that are enabled to inhibit or block tyrosine kinases, receptor tyrosine kinases included. Thus, the term includes per se ErbB receptor antagonists / inhibitors as defined above. With exception of the anti-ErbB receptor antibodies mentioned above and below, more preferable tyrosine kinase antagonist agents under this definition are chemical compounds which have shown efficacy in mono- drug therapy for eg breast and prostate cancer.
- Suitable indolocarbazole- type tyrosine kinase inhibitors can be obtained using information found in documents such as US patents 5,516,771 ; 5,654,427; 5,461 ,146; 5,650,407. US patents 5,475,110; 5,591 ,855; 5,594,009 and WO 96/11933 disclose pyrrolocarbazole-type tyrosine kinase inhibitors and prostate cancer.
- gefitinib IRESSA ® , Astra Zeneca
- a number of agents that target this receptor are in use or in development, including monoclonal antibodies (such as cetuximab) and tyrosine kinase inhibitors (such as erlotinib and gefitinib).
- monoclonal antibodies such as cetuximab
- tyrosine kinase inhibitors such as erlotinib and gefitinib.
- EGFR inhibitors tyrosine kinase inhibitors
- the most common adverse effect common to EGFR inhibitors is an acne-form rash, usually on the face and upper torso. Skin rash occurs in 45-100% of patients, with a rapid onset in the majority of patients, detectable after approximately 7-10 days of treatment and reaching a maximum after 2-3 weeks (Robert et al., Lancet Oncol. 491, 2005).
- EGFR is primarily expressed in proliferating, undifferentiated keratinocytes of the basal layer of the epidermis and the outer root sheet of the hair follicle (Nanney et al., J. Invest. Dermatol. 83:385, 1984). Alterations in EGFR expression and activity have been linked to abnormal epidermal growth and differentiation (Murillas R et al., EMBO J 1995; Sibilia M et al.,Cell 2000; King LE et al., J Invest Dermatol. 1990).
- Keratinocytes are stratified, squamous, epithelial cells which comprise skin and mucosa, including oral, esophageal, corneal, conjunctival, and genital epithelia. Keratinocytes provide a barrier between the host and the environment. They prevent the entry of toxic substances from the environment and the loss of important constituents from the host. Keratinocytes differentiate as they progress from the basal layer to the skin surface. The normal turnover time for keratinocytes is around 30 days but epidermal turnover may be accelerated in some skin diseases such as psoriasis.
- gefitinib a small chemical compound (Iressa® ) causes up-regulation of growth arrest- and maturation- marker in the basal layer of the epidermis. Therefore, it may be possible that cell cycle arrest and maturation of keratinocyte cause skin rash since altered differentiation of keratinocytes may lead to follicular occlusions as observed in patients (Albanell et al., J. Clin. Oncol. 20:110, 2002).
- Chemokines are a family of structurally related glycoproteins with potent leukocyte activation and/or chemotactic activity. They are 70 to 90 amino acids in length and approximately 8 to 10 kDa in molecular weight. Most of them fit into two subfamilies with four cysteine residues. These subfamilies are base on whether the two amino terminal cysteine residues are immediately adjacent or separated by one amino acid.
- the chemokines also known as CXC chemokines, contain a single amino acid between the first and second cysteine residues; ⁇ , or CC, chemokines have adjacent cysteine residues.
- CXC chemokines are chemoattractants for neutrophils whereas CC chemokines generally attract monocytes, lymphocytes, basophils, and eosinophils.
- the C group has one member (lymphotactin). It lacks one of the cysteines in the four-cysteine motif, but shares homology at its carboxyl terminus with the C-C chemokines. The C chemokine seems to be lymphocyte specific.
- the fourth subgroup is the C-X3-C subgroup.
- the C-X3-C chemokine (fractalkine/neurotactin) has three amino acid residues between the first two cysteine. It is tethered directly to the cell membrane via a long mucin stalk and induces both adhesion and migration of leukocytes.
- the invention is based on the principal finding that the treatment of EGFR related tumors by means of anti-cancer agent, preferably EGFR inhibitors, causes specific modulations of the chemokine pattern in the skin tissue as well as in the respective tumor tissue or in serum of a patient.
- the chemokines in said tissue or serum may be down- or up-regulated dependent on the nature and quantity of the anti-cancer agent used in the therapy.
- modulated chemokine expression patterns in the skin represent useful markers to predict skin rash in patients at early time points during the anti-cancer treatment, enabling clinicians to counteract rash before it can be seen.
- modulated chemokine expression patterns in the skin are more reliable surrogate markers of effective target inhibition (and thereby possibly also clinical outcome) than skin rash, as in addition to chemokine modulation, skin rash depends on the patient's individual immune system.
- patients can be analyzed within the 1 st week of treatment to pinpoint patients unlikely to benefit from anti-EGFR therapy and thereby enabling clinicians to change to alternative therapies.
- chemokine receptor blocking agents that interfere with chemokine mediated chemoattraction induced by EGFR blockade may represent novel therapeutics to manage skin disease side effects of EGFR related tumor therapy. These agents should preferentially used topical as their effects on the skin may be mirrored in the tumor.
- a method of predicting outbreak and intensity of a skin irritation, preferably skin rash, associated or correlated with cancer therapy in a patient comprising: (i) determining in a first skin tissue probe the expression pattern of chemokines with standard methods, wherein the probe is taken from a patient before starting treatment with an anti-cancer agent, which is directed against tumor cells that utilize epidermal growth factor receptor (EGFR), (ii) determining in a second skin probe derived from said patient (preferably from the same skin area) the expression pattern of chemokines, wherein the probe is taken at a time after having started the treatment with said anticancer agent (preferably 1 - 10 days, more preferably 1 - 7 days, and most preferably 5 - 7 days),
- EGFR epidermal growth factor receptor
- step (iii) optionally determining in a third and further skin probe the chemokine expression pattern, wherein the probe is taken from the patient at a later time than the respective precursor probe of step (ii),
- tissue probes of step (ii) optionally determining in a third and further tissue probe the chemokine expression pattern, wherein the probe is taken from the patient at a later time than the respective precursor probe of step (ii), (iv) comparing the respective chemokine expression patterns of the tissue probes of step (ii) and optionally (iii) with the expression pattern of the tissue probe of step (i), and determining thereof which chemokines have been changed in quality and/ or quantity in probe (ii) and (iii) relative to the cemokine pattern of the reference probe of (i) or the respective precursor probe; (v) predicting from the changes in the chemokine pattern of said tissue probes the likelihood and intensity of the tumoral response of the patient to the treatment with said anti-cancer agent.
- the chemokine pattern and its relative change, respectively, not only in the tumor tissue but also in the skin tissue of the patient is correlated to the tumor response.
- a method of determining the optimum dose of an anti-cancer agent for the treatment of cancer in a patient comprising:
- the further treatment with the anticancer agent is either obsolete or, alternatively, the dosis should be increased until an effect in the chemokine pattern can be observed.
- step (ii) is taken within 1 - 10 days after onset of the treatment with said anti-cancer agent.
- step (ii) is taken within 2 - 7 days after onset of the treatment with said anti-cancer agent.
- the anti-cancer agent is an EGFR inhibitor.
- the anti-EGFR antibody is Mab c225 (cetuximab) or Mab h425 (EMD72000, matuzumab).
- chemokines • A corresponding method, wherein at least one of the following chemokines are involved: IL-8, MCP-1 , RANTES and IP-10.
- EGFR inhibitor more preferably an anti-EGFR antibody, such as Mab c225 (cetuximab) or Mab h425 (EMD72000, matuzumab), wherein preferably at least one of the following chemokines are involved: IL-8, MCP-1 , RANTES and IP-10.
- an anti-EGFR antibody such as Mab c225 (cetuximab) or Mab h425 (EMD72000, matuzumab
- chemokines are involved: IL-8, MCP-1 , RANTES and IP-10.
- chemokines which are up- or down-regulated in-vivo during cancer treatment with an anti-cancer agent, as a diagnostic marker for determining the efficiency of said treatment, and / or the likelihood of the occurrence of skin irritations, preferably skin rash, accompanied by said treatment, wherein said cancer utilizes / overexpresses EGFR and said anti-cancer agent is an EGFR inhibitor, preferably an anti-EGFR antibody, such as Mab c225
- cetuximab or Mab h425 (EMD72000, matuzumab).
- chemokines which are up- or down-regulated in-vivo during cancer treatment with an anti-cancer agent, for identification of a target upstream of said chemokine expression, suitable for the development and manufacture of a drug targeting said target for the treatment of cancer that utilizes / overexpresses EGFR in the patient solely or in combination with said anticancer agent, wherein said anti-cancer agent is an EGFR inhibitor, preferably an anti-EGFR antibody, such as Mab c225 (cetuximab) or Mab h425 (EMD72000, matuzumab).
- an anti-EGFR inhibitor preferably an anti-EGFR antibody, such as Mab c225 (cetuximab) or Mab h425 (EMD72000, matuzumab).
- the current invention has shown by experimental work that blocking EGF receptor, for example, with monoclonal antibodies, such as cetuximab (mAb c225) or mAb h425 (matuzumab) or tyrosine kinase inhibitors (gefitinib, Iressa®), interferes with EGFR-dependent signaling cascades in primary keratinocytes.
- monoclonal antibodies such as cetuximab (mAb c225) or mAb h425 (matuzumab) or tyrosine kinase inhibitors (gefitinib, Iressa®)
- keratinocytes were treated with different concentrations of anti-EGFR inhibitors followed by treatment with or without TGF alpha or TNF alpha for, approximately, 24 h.
- chemokines were evaluated in conditioned medium of keratinocytes that had been treated with anti-EGFR agents for 24h. Evaluations were carried out with the Luminex bead technology. In these experiments keratinocytes were treated with anti-EGFR inihibitors followed by treatment with or without TGF alpha or TNF alpha.
- IL-8 was consistently down-regulated in response to EGFR blockade ( Figure 3), whereas RANTES and IP-10 were up-regulated ( Figure 4-5).
- IL-8 is a pro-angiogenic factor suggesting that its down-regulation interferes with blood vessel formation in the skin.
- RANTES and IP-10 have been described as chemoattractive factors for leucocytes suggesting that enhanced expression (and possibly also others chemokines) induces infiltration of leucocytes into the skin which cause inflammation and eventually skin rash.
- modulated chemokine expression pattern in response to EGFR blockade in keratinocytes and tumor tissue results in migration / chemoattraction of leucocytes that can be inhibited by agents/ drugs interfering with these chemokines.
- Experiments include chemotaxis assays in which conditioned medium of keratinocyte is collected after 24 h of treatment with EGFR inhibitors and stimulation with or without TGF alpha or TNF alpha. The conditioned medium is placed into the lower chamber and freshly islated PBMC or granulocytes are placed into the upper chamber of a Boyden chamber.
- Chemotaxis of blood cells is then monitored by measuring the amount of PBMC or granulocytes in the lower chamber after speficic times.
- PBMC or granulocytes are activated in vitro beforehand to enhance the migratory activity of the cells.
- the chemokines within the conditioned medium cause chemotaxis of PBMC and granulocytes and that this represents part of the biological reaction seen in skin rash.
- specific inhibitors to chemokine receptors are added to the conditioned medium into the lower chamber of the Boyden chamber and chemotaxis is evaluated in comparison to conditioned medium only.
- chemotaxis is induced by chemokine / chemokine receptor interaction and that blockade of this interaction by chemokine receptor anatagonists interferes with chemotaxis of blood cells.
- Modulated chemokine expression in response to EGFR blockade results in migration/chemoattraction of leucocytes into the skin of mice. Furthermore, leucocyte infiltration can be analyzed and correlated with chemokine expression. It has been furthermore shown according to the invention that chemokine levels within the skin of mice are modulated following treatment with anti-EGFR inhibitors, and that this modulation is accompanied by leucocyte infiltration. Systemic administration of chemokine receptor antagonists have been shown to interfere with leucocyte infiltration into the skin of animals treated with anti-EGFR therapy, and thus reduces/abolishes development of skin rash.
- individuals can be treated with anti-EGFR agents until first signs of skin toxcitity can bee seen and then the affected diseased skin can be treated topically with anti-chemokine receptor agents to interfere with leucocyte infiltration and reduce skin toxicity.
- Topical administration of chemokine receptor antagonists onto the skin reduces leucocyte infiltration and skin toxicity. It is suggested that these agents could be used in clinical practice to treat skin rash of patients undergoing anti-EGFR therapy.
- chemokines levels in the skin of patients can be analyzed within the first week or first ten days following treatment with anti-EGFR antagonists to find out whether chemokines expression is modulated in response to EGFR blockade. This can be done by analyzing skin biopsies prior and on- treatment. Chemokine levels are modulated in response to anti-EGFR therapy and the degree of modulation can be taken as a diagnostic measure to predict if the patient will respond to the treatment. It is suggested that patients that have no or little chemokine modulation are treated at non-optimal doses with anti-EGFR inhibitors, and that doses should be elevated until a modulation is seen.
- patients without chemokine modulation could be transferred to a different therapy.
- the modulation discovererd by the inventors plays an important role in the development of skin rash in this context and could therefore be used as:
- the present invention suggests that the inhibition of EGFR signaling in carcinomas causes changes in the carcinoma chemokine milieu and affects the inflammatory status of the tumor with tumor growth inhibition as consequence.
- chemokines are regulated in a similar way in tumor cells and primary keratinocytes in vitro.
- keratinocytes experiments were conducted that showed that blocking EGF receptor with monoclonal antibodies, such as cetuximab or EMD72000 or tyrosine kinase inhibitors (gefitinib, Iressa®) interferes with EGFR-dependent signaling cascades in various tumor cell lines such as A431 representing different tumor indications.
- tumor cell lines such as A431 modulates chemokine expression in vitro.
- Tumor cells were treated with anti- EGFR agents and this was followed by treatment with or without TGF alpha or TNF alpha.
- Secreted chemokines were evaluated in conditioned medium of tumor cells obtained after 24 h. Evaluation was carried out with the Luminex bead technology. Similar to the data obtained with primary keratinocytes, several chemokines were modulated in response to EGFR blockade in tumor cells. IL-8 was consistently down-regulated ( Figure 6), whereas RANTES and IP-10 were up-regulated in tumor cells that were treated with EGFR inhibitors ( Figure 7-8).
- IL-8 Modulation of IL-8 was evaluated in a panel of different tumor cell lines and was found to be consistently down-regulated in response to EGFR blockade (Figure 9). This suggests that levels of IL-8 are a biomarker of an effective anti-EGFR therapy. It is anticipated to evaluate levels of IL-8 in blood of patients undergoing anti-EGFR therapy, and to use decreased levels as a diagnostic measure to monitor pharmacodynamic effects of anti-EGFR agents.
- modulated chemokine expression pattern in response to EGFR blockade in tumor tissue results in migration/chemoattraction of leucocytes.
- chemotaxis assays in which conditioned medium of tumor cells is collected after 24 h of treatment with EGFR inhibitors and stimulation with or without TGF alpha or TNF alpha.
- the conditioned medium is placed into the lower chamber and freshly islated PBMC or granulocytes are placed into the upper chamber of a Boyden chamber.
- Chemotaxis of blood cells is then monitored by measuring the amount of PBMC or granulocytes in the lower chamber after specific times.
- PBMC or granulocytes are activated in vitro beforehand to enhance the migratory activity of the cells. These experiments show that the chemokines within the conditioned medium cause chemotaxis of PBMC and granulocytes.
- chemokines which are responsible for the chemotactic events
- specific inhibitors to chemokine receptors are added to the conditioned medium into the lower chamber of the Boyden chamber and chemotaxis is evaluated in comparison to conditioned medium only.
- Chemotaxis is induced by chemokine/chemokine receptor interaction and, blockade of this interaction by chemokine receptor anatagonists interferes with chemotaxis of blood cells.
- Keratinocytes were treated with EGFR inhibitors (Cetuximab, Matuzumab or Iressa), and stimulated with or without different growth factors (TGFa or TNFa).
- EGFR inhibitors Cetuximab, Matuzumab or Iressa
- TGFa or TNFa different growth factors
- Iressa and stimulated with or without different growth factors (TGFa or TNFa).
- Keratinocytes were treated with EGFR inhibitors (Cetuximab, Matuzumab or
- Iressa and stimulated with or without different growth factors (TGFa or TNFa).
- Keratinocytes were treated with EGFR inhibitors (Cetuximab, Matuzumab or
- Iressa and stimulated with or without different growth factors (TGFa or TNFa).
- Keratinocytes were treated with EGFR inhibitors (Cetuximab, Matuzumab or Iressa), and stimulated with or without different growth factors (TGFa or TNFa). After 24 h, cellular supernatants were collected and levels of IP-10 were quantified using the Luminex technology. Analyses revealed that treatment with EGFR inhibitors up-regulated IP-10 in a dose-dependent manner.
- A431 were treated with EGFR inhibitors (Cetuximab, Matuzumab or Iressa), and stimulated with or without different growth factors (TGFa or TNFa). After 24 h, cellular supernatants were collected and levels of IL-8 were quantified using the Luminex technology. Analyses revealed that treatment with EGFR inhibitors down-regulated IL-8 in a dose-dependent manner.
- A431 were treated with EGFR inhibitors (Cetuximab, Matuzumab or Iressa), and stimulated with or without different growth factors (TGFa or TNFa). After 24 h, cellular supernatants were collected and levels of RANTES were quantified using the Luminex technology. Analyses revealed that treatment with EGFR inhibitors up-regulated RANTES in a dose-dependent manner.
- A431 were treated with EGFR inhibitors (Cetuximab, Matuzumab or Iressa), and stimulated with or without different growth factors (TGFa or TNFa). After 24 h, cellular supernatants were collected and levels of IP-10 were quantified using the Luminex technology. Analyses revealed that treatment with EGFR inhibitors up- regulated IP-10 in a dose-dependent manner.
- IL-8 Modulation of secreted IL-8 levels in different tumor cell lines.
- Different tumor cell lines DiFi, HT29, A431, MCF-7, PC-3 and U87MG
- EGFR inhibitors Cetuximab or Matuzumab
- TGFa TGFa
- cellular supernatants were collected and levels of IL-8 were quantified using the Luminex technology. Analyses revealed that treatment with EGFR inhibitors down-regulated IL-8 in all tumor cell lines investigated in a dose- dependent manner.
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Priority Applications (9)
Application Number | Priority Date | Filing Date | Title |
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JP2008534930A JP2009511524A (en) | 2005-10-11 | 2006-10-11 | EGFR-dependent regulation of chemokine expression and the effect of chemokine expression regulation on the treatment and diagnosis of tumors and their side effects |
US12/089,819 US20080199850A1 (en) | 2005-10-11 | 2006-10-11 | Egfr Dependent Modulation of Chemokine Expression and Influence on Therapy and Diagnosis of Tumors and Side Effects Thereof |
AU2006301518A AU2006301518B2 (en) | 2005-10-11 | 2006-10-11 | EGFR dependent modulation of chemokine expression and influence on therapy and diagnosis of tumors and side effects thereof |
EP06806198A EP1934599A1 (en) | 2005-10-11 | 2006-10-11 | Egfr dependent modulation of chemokine expression and influence on therapy and diagnosis of tumors and side effects thereof |
EA200800963A EA014433B1 (en) | 2005-10-11 | 2006-10-11 | Egfr dependent modulation of chemokine expression and influence on therapy and diagnosis of tumors and side effects thereof |
CA002625291A CA2625291A1 (en) | 2005-10-11 | 2006-10-11 | Egfr dependent modulation of chemokine expression and influence on therapy and diagnosis of tumors and side effects thereof |
BRPI0617236-9A BRPI0617236A2 (en) | 2005-10-11 | 2006-10-11 | egfr-dependent modulation of chemokine expression and influence on tumor therapy and diagnosis and side effects |
IL190537A IL190537A (en) | 2005-10-11 | 2008-03-31 | Egfr dependent modulation of chemokine expression and influence on therapy and diagnosis of tumors and side effects thereof |
US12/986,490 US20110244506A1 (en) | 2005-10-11 | 2011-01-07 | Egfr dependent modulation of chemokine expression and influence on therapy and diagnosis of tumors and side effects thereof |
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Cited By (2)
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WO2008132608A2 (en) * | 2007-04-26 | 2008-11-06 | University Of Memphis Research Foundation | Combined use of egf pathway inhibitors and differentiation promoting compounds |
JP2009538607A (en) * | 2006-06-02 | 2009-11-12 | グラクソスミスクライン バイオロジカルズ ソシエテ アノニム | How to identify whether a patient is a responder to immunotherapy |
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EP2335694B1 (en) | 2003-09-15 | 2018-06-13 | NanoPharmaceuticals LLC | Thyroid hormone analogs and methods of use |
US8668926B1 (en) | 2003-09-15 | 2014-03-11 | Shaker A. Mousa | Nanoparticle and polymer formulations for thyroid hormone analogs, antagonists, and formulations thereof |
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US10130686B2 (en) * | 2005-09-15 | 2018-11-20 | Nanopharmaceuticals Llc | Method and composition of thyroid hormone analogues and nanoformulations thereof for treating inflammatory disorders |
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US10980420B2 (en) | 2016-01-26 | 2021-04-20 | Stryker European Operations Limited | Configurable platform |
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CA3026504A1 (en) | 2016-06-07 | 2017-12-14 | Nanopharmaceuticals, Llc | Non-cleavable polymer conjugated with .alpha..nu..beta.3 integrin thyroid antagonists |
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JP6931705B2 (en) | 2017-02-10 | 2021-09-08 | ノバダック テクノロジーズ ユーエルシー | Open Field Handheld Fluorescence Imaging Systems and Methods |
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US10961204B1 (en) | 2020-04-29 | 2021-03-30 | Nanopharmaceuticals Llc | Composition of scalable thyrointegrin antagonists with improved blood brain barrier penetration and retention into brain tumors |
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- 2006-10-11 CN CNA2006800377266A patent/CN101283275A/en active Pending
- 2006-10-11 EP EP10008588A patent/EP2251688A1/en not_active Withdrawn
- 2006-10-11 WO PCT/EP2006/009837 patent/WO2007042286A1/en active Application Filing
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Cited By (4)
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JP2009538607A (en) * | 2006-06-02 | 2009-11-12 | グラクソスミスクライン バイオロジカルズ ソシエテ アノニム | How to identify whether a patient is a responder to immunotherapy |
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WO2008132608A2 (en) * | 2007-04-26 | 2008-11-06 | University Of Memphis Research Foundation | Combined use of egf pathway inhibitors and differentiation promoting compounds |
WO2008132608A3 (en) * | 2007-04-26 | 2009-04-30 | Univ Memphis Res Foundation | Combined use of egf pathway inhibitors and differentiation promoting compounds |
Also Published As
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BRPI0617236A2 (en) | 2011-07-19 |
IL190537A0 (en) | 2008-11-03 |
KR20080068848A (en) | 2008-07-24 |
ZA200804006B (en) | 2009-03-25 |
EA200800963A1 (en) | 2008-10-30 |
EA014433B1 (en) | 2010-12-30 |
IL190537A (en) | 2011-04-28 |
JP2009511524A (en) | 2009-03-19 |
CN101283275A (en) | 2008-10-08 |
AU2006301518B2 (en) | 2012-05-17 |
US20110244506A1 (en) | 2011-10-06 |
EP2251688A1 (en) | 2010-11-17 |
EP1934599A1 (en) | 2008-06-25 |
CA2625291A1 (en) | 2007-04-19 |
US20080199850A1 (en) | 2008-08-21 |
AU2006301518A1 (en) | 2007-04-19 |
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