WO2007024264A3 - Method and system for digital image based tissue independent simultaneous nucleus, cytoplasm and membrane quantitation - Google Patents

Method and system for digital image based tissue independent simultaneous nucleus, cytoplasm and membrane quantitation Download PDF

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Publication number
WO2007024264A3
WO2007024264A3 PCT/US2006/006530 US2006006530W WO2007024264A3 WO 2007024264 A3 WO2007024264 A3 WO 2007024264A3 US 2006006530 W US2006006530 W US 2006006530W WO 2007024264 A3 WO2007024264 A3 WO 2007024264A3
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WO
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Patent type
Prior art keywords
cytoplasm
pixels
quantitation
nucleus
membrane
Prior art date
Application number
PCT/US2006/006530
Other languages
French (fr)
Other versions
WO2007024264A2 (en )
Inventor
Abhijeet S Gholap
Gauri A Gholap
Aparna Joshi
Amitabha Basu
C V K Rao
Prithviraj Jadhav
Original Assignee
Bioimagene Inc
Abhijeet S Gholap
Gauri A Gholap
Aparna Joshi
Amitabha Basu
C V K Rao
Prithviraj Jadhav
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    • GPHYSICS
    • G06COMPUTING; CALCULATING; COUNTING
    • G06TIMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00Image analysis
    • G06T7/0002Inspection of images, e.g. flaw detection
    • G06T7/0012Biomedical image inspection
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/645Specially adapted constructive features of fluorimeters
    • G01N21/6456Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458Fluorescence microscopy
    • GPHYSICS
    • G06COMPUTING; CALCULATING; COUNTING
    • G06KRECOGNITION OF DATA; PRESENTATION OF DATA; RECORD CARRIERS; HANDLING RECORD CARRIERS
    • G06K9/00Methods or arrangements for reading or recognising printed or written characters or for recognising patterns, e.g. fingerprints
    • G06K9/00127Acquiring and recognising microscopic objects, e.g. biological cells and cellular parts
    • G06K9/0014Pre-processing, e.g. image segmentation ; Feature extraction
    • GPHYSICS
    • G06COMPUTING; CALCULATING; COUNTING
    • G06TIMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00Image analysis
    • G06T7/10Segmentation; Edge detection
    • G06T7/11Region-based segmentation
    • GPHYSICS
    • G06COMPUTING; CALCULATING; COUNTING
    • G06TIMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00Indexing scheme for image analysis or image enhancement
    • G06T2207/10Image acquisition modality
    • G06T2207/10056Microscopic image
    • GPHYSICS
    • G06COMPUTING; CALCULATING; COUNTING
    • G06TIMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00Indexing scheme for image analysis or image enhancement
    • G06T2207/10Image acquisition modality
    • G06T2207/10064Fluorescence image
    • GPHYSICS
    • G06COMPUTING; CALCULATING; COUNTING
    • G06TIMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00Indexing scheme for image analysis or image enhancement
    • G06T2207/30Subject of image; Context of image processing
    • G06T2207/30004Biomedical image processing
    • G06T2207/30024Cell structures in vitro; Tissue sections in vitro

Abstract

A method and system for automatic digital image based tissue independent simultaneous nucleus, cytoplasm and membrane quantitation. Plural types of pixels comprising cell components including at least cell cytoplasm and cell membranes from a biological tissue sample to which a chemical compound has been applied and has been processed to remove background pixels and pixels including counterstained components are simultaneously identified. The identified cell components pixels are automatically classified to determine a medical conclusion such as a human breast cancer, a human prostrate cancer or an animal cancer.
PCT/US2006/006530 2005-02-23 2006-02-23 Method and system for digital image based tissue independent simultaneous nucleus, cytoplasm and membrane quantitation WO2007024264A3 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US65546505 true 2005-02-23 2005-02-23
US60/655,465 2005-02-23

Publications (2)

Publication Number Publication Date
WO2007024264A2 true WO2007024264A2 (en) 2007-03-01
WO2007024264A3 true true WO2007024264A3 (en) 2007-04-19

Family

ID=36676002

Family Applications (1)

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PCT/US2006/006530 WO2007024264A3 (en) 2005-02-23 2006-02-23 Method and system for digital image based tissue independent simultaneous nucleus, cytoplasm and membrane quantitation

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WO (1) WO2007024264A3 (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8137912B2 (en) 2006-06-14 2012-03-20 The General Hospital Corporation Methods for the diagnosis of fetal abnormalities
US8168389B2 (en) 2006-06-14 2012-05-01 The General Hospital Corporation Fetal cell analysis using sample splitting
US8195415B2 (en) 2008-09-20 2012-06-05 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuploidy by sequencing
US8921102B2 (en) 2005-07-29 2014-12-30 Gpb Scientific, Llc Devices and methods for enrichment and alteration of circulating tumor cells and other particles
US9240043B2 (en) 2008-09-16 2016-01-19 Novartis Ag Reproducible quantification of biomarker expression

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5425762B2 (en) 2007-05-14 2014-02-26 ヒストロックス,インコーポレイテッド.Historx,Inc. Compartments separated by pixel evaluation using image data clustering
CA2604317C (en) 2007-08-06 2017-02-28 Historx, Inc. Methods and system for validating sample images for quantitative immunoassays

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003030088A1 (en) * 2001-10-04 2003-04-10 Chromavision Medical Systems, Inc. Method and apparatus for scoring the uptake of markers in cells

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003030088A1 (en) * 2001-10-04 2003-04-10 Chromavision Medical Systems, Inc. Method and apparatus for scoring the uptake of markers in cells

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
TABESH A ET AL: "Automated prostate cancer diagnosis and Gleason grading of tissue microarrays", PROCEEDINGS OF THE SPIE, SPIE, BELLINGHAM, VA, US, vol. 5747, no. 1, 17 February 2005 (2005-02-17), pages 58 - 70, XP002408570, ISSN: 0277-786X *
TEVEROVSKIY M ET AL: "Improved prediction of prostate cancer recurrence based on an automated tissue image analysis system", BIOMEDICAL IMAGING: MACRO TO NANO, 2004. IEEE INTERNATIONAL SYMPOSIUM ON ARLINGTON,VA, USA APRIL 15-18, 2004, PISCATAWAY, NJ, USA,IEEE, 15 April 2004 (2004-04-15), pages 257 - 260, XP010773846, ISBN: 0-7803-8389-3 *

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8921102B2 (en) 2005-07-29 2014-12-30 Gpb Scientific, Llc Devices and methods for enrichment and alteration of circulating tumor cells and other particles
US8168389B2 (en) 2006-06-14 2012-05-01 The General Hospital Corporation Fetal cell analysis using sample splitting
US9347100B2 (en) 2006-06-14 2016-05-24 Gpb Scientific, Llc Rare cell analysis using sample splitting and DNA tags
US9273355B2 (en) 2006-06-14 2016-03-01 The General Hospital Corporation Rare cell analysis using sample splitting and DNA tags
US8372584B2 (en) 2006-06-14 2013-02-12 The General Hospital Corporation Rare cell analysis using sample splitting and DNA tags
US9017942B2 (en) 2006-06-14 2015-04-28 The General Hospital Corporation Rare cell analysis using sample splitting and DNA tags
US8137912B2 (en) 2006-06-14 2012-03-20 The General Hospital Corporation Methods for the diagnosis of fetal abnormalities
US9240043B2 (en) 2008-09-16 2016-01-19 Novartis Ag Reproducible quantification of biomarker expression
US8682594B2 (en) 2008-09-20 2014-03-25 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuploidy by sequencing
US8296076B2 (en) 2008-09-20 2012-10-23 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuoploidy by sequencing
US8195415B2 (en) 2008-09-20 2012-06-05 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuploidy by sequencing
US9353414B2 (en) 2008-09-20 2016-05-31 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuploidy by sequencing
US9404157B2 (en) 2008-09-20 2016-08-02 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuploidy by sequencing

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Publication number Publication date Type
WO2007024264A2 (en) 2007-03-01 application

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