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WO2007011622A3 - Microfluidic methods and apparatuses for sample preparation and analysis - Google Patents

Microfluidic methods and apparatuses for sample preparation and analysis

Info

Publication number
WO2007011622A3
WO2007011622A3 PCT/US2006/027111 US2006027111W WO2007011622A3 WO 2007011622 A3 WO2007011622 A3 WO 2007011622A3 US 2006027111 W US2006027111 W US 2006027111W WO 2007011622 A3 WO2007011622 A3 WO 2007011622A3
Authority
WO
Grant status
Application
Patent type
Prior art keywords
fluid
targets
channel
carrier
portion
Prior art date
Application number
PCT/US2006/027111
Other languages
French (fr)
Other versions
WO2007011622A2 (en )
Inventor
Rudolf Gilmanshin
Jonathan W Larson
Gregory R Yantz
Original Assignee
Rudolf Gilmanshin
Jonathan W Larson
Us Genomics Inc
Gregory R Yantz
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date

Links

Classifications

    • BPERFORMING OPERATIONS; TRANSPORTING
    • B82NANOTECHNOLOGY
    • B82YSPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y10/00Nanotechnology for information processing, storage or transmission, e.g. quantum computing or single electron logic
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502776Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for focusing or laminating flows
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B82NANOTECHNOLOGY
    • B82YSPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y5/00Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06Fluid handling related problems
    • B01L2200/0636Focussing flows, e.g. to laminate flows
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06Fluid handling related problems
    • B01L2200/0647Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0663Stretching or orienting elongated molecules or particles
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/04Moving fluids with specific forces or mechanical means
    • B01L2400/0475Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics

Abstract

Microfludic channels are constructed for use in preparing and/or analyzing samples. In one embodiment, a microfluidic channel receives a carrier fluid having both non-targets and targets. The non-targets are moved from the carrier fluid by diffusion and into sheathing fluids also present in the channel before contents of the carrier fluid are analyzed. In another embodiment, a microchannel delivers multiple fluid samples from an upstream portion to a downstream portion of the channel where each of the fluid samples are separated from one another by a sheathing fluid that also travels toward the downstream portion. In another embodiment, a microchannel is constructed to create multiple elongational flows in series that can align polymers from a coiled state.
PCT/US2006/027111 2005-07-18 2006-07-13 Microfluidic methods and apparatuses for sample preparation and analysis WO2007011622A3 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
US70068905 true 2005-07-18 2005-07-18
US70048005 true 2005-07-18 2005-07-18
US70049005 true 2005-07-18 2005-07-18
US60/700,480 2005-07-18
US60/700,490 2005-07-18
US60/700,689 2005-07-18

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP20060787065 EP1904653A4 (en) 2005-07-18 2006-07-13 Microfluidic methods and apparatuses for sample preparation and analysis
JP2008522826A JP2009501938A (en) 2005-07-18 2006-07-13 The methods and microfluidic apparatus according to microfluidization for sample preparation and analysis

Publications (2)

Publication Number Publication Date
WO2007011622A2 true WO2007011622A2 (en) 2007-01-25
WO2007011622A3 true true WO2007011622A3 (en) 2007-05-24

Family

ID=37669351

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/027111 WO2007011622A3 (en) 2005-07-18 2006-07-13 Microfluidic methods and apparatuses for sample preparation and analysis

Country Status (3)

Country Link
EP (1) EP1904653A4 (en)
JP (1) JP2009501938A (en)
WO (1) WO2007011622A3 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9360476B2 (en) 2006-12-19 2016-06-07 Fio Corporation Microfluidic system and method to test for target molecules in a biological sample
US9459200B2 (en) 2008-08-29 2016-10-04 Fio Corporation Single-use handheld diagnostic test device, and an associated system and method for testing biological and environmental test samples

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2694541A1 (en) * 2007-07-16 2009-01-22 California Institute Of Technology Microfluidic devices, methods and systems for detecting target molecules
JP5628037B2 (en) 2007-10-12 2014-11-19 フィオ コーポレイション Flow focusing method and system for forming a concentrated microbeads and microbead formed on its system
EP2321810A4 (en) 2008-06-25 2014-09-17 Fio Corp Bio-threat alert system
CA2749660C (en) 2009-01-13 2017-10-31 Fio Corporation A handheld diagnostic test device and method for use with an electronic device and a test cartridge in a rapid diagnostic test
US8202486B2 (en) * 2009-08-12 2012-06-19 Caliper Life Sciences, Inc. Pinching channels for fractionation of fragmented samples
US9139426B2 (en) 2010-09-21 2015-09-22 The University Of North Carolina At Chapel Hill Methods, systems and devices for forming nanochannels
EP2812708A4 (en) * 2012-02-10 2015-09-30 Univ North Carolina Devices with fluidic nanofunnels, associated methods, fabrication and analysis systems
CA2902903A1 (en) * 2013-02-28 2014-09-04 The University Of North Carolina At Chapel Hill Nanofluidic devices with integrated components for the controlled capture, trapping, and transport of macromolecules and related methods of analysis
US9255288B2 (en) 2013-03-13 2016-02-09 The University Of North Carolina At Chapel Hill Nanofluidic devices for the rapid mapping of whole genomes and related systems and methods of analysis

Citations (8)

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Publication number Priority date Publication date Assignee Title
US4793705A (en) * 1987-10-07 1988-12-27 The United States Of America As Represented By The United States Department Of Energy Single molecule tracking
US5274240A (en) * 1990-01-12 1993-12-28 The Regents Of The University Of California Capillary array confocal fluorescence scanner and method
US5356776A (en) * 1991-09-10 1994-10-18 Hitachi, Ltd. DNA measuring method
US5674743A (en) * 1993-02-01 1997-10-07 Seq, Ltd. Methods and apparatus for DNA sequencing
US6355420B1 (en) * 1997-02-12 2002-03-12 Us Genomics Methods and products for analyzing polymers
US6790671B1 (en) * 1998-08-13 2004-09-14 Princeton University Optically characterizing polymers
US6818395B1 (en) * 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US7118910B2 (en) * 2001-11-30 2006-10-10 Fluidigm Corporation Microfluidic device and methods of using same

Family Cites Families (6)

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US6506609B1 (en) * 1999-05-17 2003-01-14 Caliper Technologies Corp. Focusing of microparticles in microfluidic systems
US6770182B1 (en) * 2000-11-14 2004-08-03 Sandia National Laboratories Method for producing a thin sample band in a microchannel device
WO2003000418A3 (en) * 2001-06-20 2003-03-13 Sebastian Boehm Microfluidic system including a virtual wall fluid interface port for interfacing fluids with the microfluidic system
US7318902B2 (en) * 2002-02-04 2008-01-15 Colorado School Of Mines Laminar flow-based separations of colloidal and cellular particles
EP1380337B1 (en) * 2002-07-12 2012-11-14 Tosoh Corporation Fine channel device and a chemically operating method for fluid using the device
JP2006522940A (en) * 2003-04-10 2006-10-05 ユー.エス. ジェノミクス, インコーポレイテッド Operation of the polymer in the microchannel

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4793705A (en) * 1987-10-07 1988-12-27 The United States Of America As Represented By The United States Department Of Energy Single molecule tracking
US5274240A (en) * 1990-01-12 1993-12-28 The Regents Of The University Of California Capillary array confocal fluorescence scanner and method
US5356776A (en) * 1991-09-10 1994-10-18 Hitachi, Ltd. DNA measuring method
US5674743A (en) * 1993-02-01 1997-10-07 Seq, Ltd. Methods and apparatus for DNA sequencing
US6355420B1 (en) * 1997-02-12 2002-03-12 Us Genomics Methods and products for analyzing polymers
US6790671B1 (en) * 1998-08-13 2004-09-14 Princeton University Optically characterizing polymers
US6818395B1 (en) * 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US6911345B2 (en) * 1999-06-28 2005-06-28 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US7118910B2 (en) * 2001-11-30 2006-10-10 Fluidigm Corporation Microfluidic device and methods of using same

Non-Patent Citations (1)

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Title
See also references of EP1904653A2 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9360476B2 (en) 2006-12-19 2016-06-07 Fio Corporation Microfluidic system and method to test for target molecules in a biological sample
US9459200B2 (en) 2008-08-29 2016-10-04 Fio Corporation Single-use handheld diagnostic test device, and an associated system and method for testing biological and environmental test samples

Also Published As

Publication number Publication date Type
WO2007011622A2 (en) 2007-01-25 application
JP2009501938A (en) 2009-01-22 application
EP1904653A4 (en) 2010-04-14 application
EP1904653A2 (en) 2008-04-02 application

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