WO2007003068A1 - Additif pour nourriture, procédé de préparation de celui-ci et application de celui-ci - Google Patents

Additif pour nourriture, procédé de préparation de celui-ci et application de celui-ci Download PDF

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Publication number
WO2007003068A1
WO2007003068A1 PCT/CN2005/000960 CN2005000960W WO2007003068A1 WO 2007003068 A1 WO2007003068 A1 WO 2007003068A1 CN 2005000960 W CN2005000960 W CN 2005000960W WO 2007003068 A1 WO2007003068 A1 WO 2007003068A1
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Prior art keywords
feed additive
feed
proanthocyanidins
oligomeric
condensed tannin
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PCT/CN2005/000960
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English (en)
Chinese (zh)
Inventor
Xin Wang
Manli Wang
Yanjun Cheng
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Hangzhou Lixin Biotechnology Co. Ltd.
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Priority to PCT/CN2005/000960 priority Critical patent/WO2007003068A1/fr
Publication of WO2007003068A1 publication Critical patent/WO2007003068A1/fr

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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/111—Aromatic compounds
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/179—Colouring agents, e.g. pigmenting or dyeing agents
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/189—Enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
    • A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry

Definitions

  • the invention relates to a feed additive and its preparation and use.
  • the feed additive is an oligomeric proanthocyanidin which can effectively reduce the infection rate and mortality of avian animals against coccidiosis at an added amount of 5-600 ppm. Background technique
  • proanthocyanidins are substances that produce anthocyanidin in the treatment of hot acid, including monomeric proanthocyanidins and polymer proanthocyanidins.
  • the monomeric proanthocyanidin corresponds to flavan-4-ol and xanthine-3,4-alcohol
  • the polymer proanthocyanidin corresponds to condensed tannin, red powder and phenolic acid.
  • Polymer proanthocyanidins are new health products and medicines that have become more and more popular in the public in recent years. It is highly resistant to oxidation and exhibits outstanding effects in terms of antiviral and anti-cancer effects.
  • Proanthocyanidins are widely found in natural plants and are the main active constituents of plant defense systems. They are also the main active components in many Chinese herbal medicines. Chinese herbal medicines such as betel nut, crane grass and pomegranate peel contain a large amount of polymer proanthocyanidins. .
  • Chinese herbal medicines such as betel nut, crane grass and pomegranate peel contain a large amount of polymer proanthocyanidins. .
  • the protective effect of grape seed tannin and tea polyphenol on the human cardiovascular system and the special physiological and biochemical activities of antibacterial, antiviral, antioxidation and anticancer have been scientifically confirmed.
  • the extraction of proanthocyanidins from plants, especially grape seed and pine bark, has resulted in large-scale production.
  • polymer proanthocyanidins Another major product of polymer proanthocyanidins is various types of tannin extracts extracted from bark, such as bayberry tannin, lozengein, black wattle, and eucalyptus.
  • These polymeric proanthocyanidins known as condensed tannins or tannins, are products of the condensation of dimeric anthocyanins with flavan-3,4-alcohol.
  • the black wattle bark tannin structural unit is the original ring thorn of the A ring which is a resorcinol type, and the average degree of polymerization is 4 to 5.
  • the structural unit of larch tannin is pyrogallol-like proanthocyanidin, and the average degree of polymerization is 9-10.
  • Maoyangmei tannin The structural unit of Maoyangmei tannin is the original vermilion, with an average degree of polymerization of 12. Condensed tannins or tannins have a high average degree of polymerization, and low levels of oligomeric proanthocyanidins are the main cause of protein precipitation and palate.
  • proanthocyanidins Common methods for taking proanthocyanidins include water extraction, organic solvent extraction, and supercritical carbon dioxide extraction. Due to the different extraction methods, the degree of polymerization of proanthocyanidins produced is different. The physiological activity of oligomeric proanthocyanidins (2 ⁇ 4mers) is much larger than that of proanthocyanidins (5mers or more), and the patent application number is 01109225. 4 and 03117429.
  • the invention patent of the invention is to use an organic solvent extraction method such as ethyl acetate to separate oligomers and polymers of proanthocyanidins from a mixture of anthocyanins, but the oligomeric proanthocyanidins obtained by the extraction method are obtained. The rate is low, and it is difficult to separate.
  • Coccidiosis is a very serious disease in poultry farming. Chicks usually have the highest incidence in 15-50 days of age, and the mortality rate in severe cases can reach more than 80%. The diseased chickens grow slowly and develop poorly, and the daily weight gain and egg production rate are affected. Prior to the present invention, a conventional method for preventing coccidiosis was to add anticoccidial drugs to feed or drinking water.
  • anticoccidial drugs include sodium salinomycin, monensin, sedummycin, and amprolium. , sulfamethoxidine, chlorpheniramine, sulfaquinoxaline, diclazuril, nicarbazin and benzophenone. These anticoccidial drugs have obvious curative effect, moderate price and convenient use, and have been widely used in the poultry farming industry. However, the main problem with the current use of anticoccidial drugs is -
  • Coccidiosis drugs produce resistance problems: Coccidia are highly resistant to drugs, making many anticoccidial drugs short-lived. According to the investigation of resistance to coccidia in Guangdong in 1997, 10 anticoccidial drugs commonly used in production (ball net, diclazuril, clopidol, chlorpyrifos, amprolium hydrochloride, chlorine chloride) Among phenylhydrazine, maduramycin, lasamycin, salinomycin, and Sindiramycin, 58% of the strains are sensitive to only one drug, and more than 80% of the strains are one or two Drugs are sensitive, and 14% of the strains are not sensitive to all drugs. It can be seen that the resistance of coccidia is very common, and the room for effective therapeutic treatment is getting smaller and smaller. This is also the coccidia in many areas. The main reason why the disease is difficult to control.
  • Coccidiosis problems in laying hens and breeders Due to the continuous use of anticoccidial drugs during the growing season and the growing period, the flocks may not have the opportunity to develop anti-coccidial immunity, but they cannot use anti-cocci during the laying period. Insecticides, which can cause severe coccidiosis in non-immune flocks.
  • Coccidiostats are generally chemical synthetic drugs or antibiotics. The residues of these drugs in the carcass affect the export of poultry products and meat safety in China.
  • the anti-coccidial effect of Chinese herbal extracts has been increasingly favored by the breeding industry.
  • the anti-coccidial effect of Chinese herbal medicine is non-toxic, no residue, no side effects and is not easy to cause coccidia resistance. It is a new feed additive that is green, environmentally friendly and safe.
  • Commonly used Chinese herbal anticoccidial drugs include verbena, Tianmingjing, Changshan, Huanghua, Sophora, and Pulsatilla.
  • Multimeric anthocyanins, tannins for the prevention and treatment of avian coccidiosis have been published in several patents (US Patent No. 5,135,746, US 6,106,838, WO 00/72858), but the proanthocyanidins involved in the above patents are multimeric flowers.
  • Natural tannins, and tannins are condensed tannins extracted directly from plants. Such condensed tannins have a higher degree of polymerization because they combine with proteins to form water-insoluble complexes.
  • the combination of tannins and protein in the feed results in the absorption and utilization of proteins in the feed by the animal, while tannins and Intestinal digestive enzyme binding reduces the activity of enzymes in the gut of animals.
  • the polymer proanthocyanidin is an active ingredient feed additive, and provides a method for rapidly and efficiently converting multimeric proanthocyanidins into oligomeric proanthocyanidins, and a method for using such feed additives in controlling animal coccidiosis.
  • Another object of the present invention is to provide the use of the feed additive for the preparation of a feed for the prevention and treatment of coccidiosis in animals.
  • a feed additive wherein the feed additive is an oligomeric proanthocyanidin, and the oligomeric proanthocyanidin is a 2 to 4 polymer.
  • the proanthocyanidins are a general term for a large class of polyphenol compounds widely found in nature.
  • the simplest proanthocyanidins are dimers of catechins, epicatechins, or catechins and epicatechins.
  • the two, three, and tetramers are called oligomers (Oligomeric).
  • Proanthocyanidins (abbreviated as 0PC), which is called a polymer in the form of a polymer.
  • the proanthocyanidins specifically referred to in the present invention are oligomeric proanthocyanidins (0PC), and are polymers of di-, tri-, tetra-mers which are specifically linked to the condensation reaction of flavan-3-ol and flavan-3,4-ol. High polymer proanthocyanidins are not included in the present invention.
  • the oligomeric proanthocyanidins to which the present invention relates can be obtained by conventional, well-reported extraction methods. These methods include water extraction, organic solvent extraction, and carbon dioxide supercritical extraction. Extracted objects include Grape Seeds, Grape Skin, Pine Bark, Pine Needles
  • the proanthocyanidins present in the grape seed extract are composed of two monomers, one is catechin and the other is epicatechin.
  • the chemical structures of the two monomers are already in the literature of phytochemistry. Widely reported.
  • Two-tetramer oligomeric proanthocyanidins formed by polymerization of these two monomers are used in the present invention.
  • the proanthocyanidins present in the pine bark extract are composed of three monomers, namely catechin, epicatechin and taxol, and the chemical structures of the three monomers are already in the literature of phytochemistry. Widely reported.
  • the di-tetramer oligomeric proanthocyanidins formed by the polymerization of these three monomers are used in the present invention.
  • the oligomeric proanthocyanidins of the present invention can also be obtained from oligocyanidins and condensed tannins by oxidative degradation.
  • the method is specifically as follows: the condensed tannin or the condensed tannin-containing extract is used as a raw material, and the condensed tannin is oxidatively degraded by using hydrogen peroxide or potassium chlorate at a concentration of 20 to 60% as an oxidizing agent, and the oxidation reaction condition is The pH is adjusted to 6 to 7, the temperature is controlled at 30 to 50 ° C, the oxidative degradation time is 15 to 120 minutes, and a yellow to brownish yellow translucent liquid is obtained, and the oligomeric proanthocyanidin is obtained by separation and purification.
  • the invention relates to a method for preparing the feed additive, that is, an oligomeric proanthocyanidin, which is prepared by using a condensed tannin or an extract containing condensed tannin as a raw material, and a hydrogen peroxide or potassium chlorate having a concentration of 20 to 60% as an oxidant.
  • the oxidation reaction is carried out at a pH of 6 to 7, at a temperature of 30 to 50 ° C for 15 to 120 minutes to obtain a yellow to brownish yellow translucent liquid, which is removed by conventional physical or biochemical methods, and then dried to obtain an oligomeric proanthocyanidin.
  • the amount of oxidizing agent added is usually from 1 to 4 times the volume of the condensed tannin or the extract containing the condensed tannin.
  • the condensed tannin is a polymer of flavanol
  • the molecular skeleton is C 6 ⁇ C 3 ⁇ C 6 , which is a polymer of 2 to 5 flavanol monomers.
  • the molecular weight distribution is between 500 and 3000 angstroms.
  • the condensed tannin may also be a tannin-rich vegetable tannin.
  • the condensed tannin may also be an extract of condensed tannin. Specifically, the method is as follows -
  • the feed additive can be applied to the prevention and treatment of coccidiosis in animals.
  • the addition of the oligomeric proanthocyanidins to animal feed can effectively reduce the infection rate and mortality of avian animals against coccidiosis, and has no negative impact on the growth and development of avian animals, so it can be used as a feed for treating coccidiosis in animals.
  • This kind of feeding additive can treat clinical symptoms such as blood in the stool and intestinal mucosal damage caused by coccidiosis infection, and reduce the mortality after coccidiosis infection.
  • the oligomeric proanthocyanidin is added in an amount of 5 to 600 ppm in a full-price material, and more preferably in an amount of 5 to 400 ppm. When used as a therapeutic drug, 5-600 ppm of the product of the present invention can be added to the feed.
  • the feed additive can also be added to 1% to 5% of the premix.
  • the amount added in the premix can be calculated based on the active ingredient of the compound feed.
  • the feed additive oligomer proanthocyanidins can be mixed with anti-coccidial drugs or other anti-stress feed additives which have been supplied in large quantities on the market to achieve the best effect against coccidia.
  • the purpose of mixing with other anticoccidial drugs is to reduce the concentration of antibiotics or chemically synthesized anticoccidial drugs in the feed to reduce the residues of antibiotics or chemically synthesized anticoccidial drugs in animal caries. .
  • Antibiotic compounds or chemically synthesized anticoccidial drugs include sedummycin, nicarbazin, salinomycin, mandeltamycin, monensin, etc., but are not limited to only a few mentioned Within the anticoccidial drugs.
  • the oligomeric proanthocyanidin is added in an amount of 5 to 400 ppm, and the amount of the other antibiotic compound or the chemical synthetic anticoccidial drug is half or less of the amount added alone.
  • the feed additive can also be used in combination with other antioxidants to enhance anti-coccidial efficacy.
  • Antioxidants include ascorbic acid, vitamin strontium, omega-3 fatty acids having antioxidant functions, and the like. 005% ⁇ 0. 1% ⁇ The amount of the oligo procyanidin is 0. 005% ⁇ 0. 1%.
  • the feed additive can also be used in combination with an enzyme preparation.
  • the enzyme preparation includes a variety of enzyme preparations conventionally used in feeds such as amylase, protease, xylanase, glucanase and the like. It also includes bromelain and the like which have anti-disease and therapeutic effects. Among them, the mixed use with bromelain is particularly effective. 001% ⁇ 1% ⁇ The amount of the oligosaccharide proanthocyanidin is 5 to 200 ppm, and the amount of the enzyme is different depending on the enzyme species and the activity level, and is generally 0.001% to 1%.
  • the feed additive can be used in the following animals: 1 chicken, 2 ducks, 3 babies, 4 pigeons, 5 free, 6 pigs, or ruminants.
  • the feed additive is for E. tenella, which is parasitic in the cecum, E. maxima, which is parasitic in the small intestine, and E. maxima, which is parasitic in the duodenum and the small intestine.
  • E. acervulina and E. mitis has both preventive and therapeutic effects.
  • the E. coli which is parasitic in the cecum it has better therapeutic and preventive effects.
  • Proanthocyanidins are plant extracts, which are widely used as antioxidants in human health foods as a feed additive for anticoccidial effects. It has the characteristics of non-toxic, no side effects, no residue in the body, and is not easy to produce drug resistance, which meets the requirements of today's society for green, environmentally friendly and pollution-free additives.
  • Proanthocyanidins are chemically clear in plant extracts. A class of compounds that have strong antioxidant and free radical scavenging functions.
  • Antioxidant and scavenging free radicals are one of the mechanisms of anti-coccidial action of natural extracts compared to other plant extracts that do not have clear chemical composition and function (Allen & Fetterer Cinical Microbiology Review 2002 15 : 58-65 ) ; 3) Compared with tannin, the oligomerization proanthocyanidins have a protein deposition capacity of almost 100% at a certain concentration, and the taste is degraded, which has no adverse effect on the amylase activity of the digestive tract and has no negative impact on animal growth and development. (4) The production cost is low, the method of use is simple, and the breeding cost is not greatly increased. detailed description
  • Example 1 Decomposition of larch gum to produce oligomeric proanthocyanidins
  • Example 2 Decomposition of black wattle bark tannin to produce oligomeric proanthocyanidins
  • Example 3 Extraction and decomposition of pine needles tannin from Pinus koraiensis. 10 kg of fresh pine needles are kneaded into small sections, adding 50 kg to 60 kg of water, and heating at 90 to 100 ° C for 30 minutes.
  • the average molecular weight of oligomeric proanthocyanidins was determined by internationally accepted thin layer chromatography (TLC). The specific method was as follows: The molecular weight was measured using a TLC60F silica gel plate manufactured by Merk. Prior to analysis, the silica gel plates were pretreated with isopropanol and then dried in a 130 ° oven for 2 hours. The oligomeric proanthocyanidins were concentrated under reduced pressure, and the solids were lyophilized, and 40 mg of the solid was dissolved in 10 ml of methanol.
  • the molecular weight of the oligomeric proanthocyanidins produced in the above tests was determined to be between 400 MW and 1000 ⁇ , and most of the degradation products had a molecular weight of about 800 MW.
  • Example 5 Effect of oligomeric proanthocyanidins on protein precipitation
  • oligomeric proanthocyanidins (example 1 0PC and grape seed 0PC) have a significantly reduced ability to precipitate proteins compared to deciduous rubber, and larch gum is a commercially available product.
  • Example 6 Effect of oligomeric proanthocyanidins (0PC) on intestinal enzyme activity in chicken
  • Example 5 20-day-old chicks were removed from the small intestine immediately after slaughter to prepare the 15% phosphoric acid buffer suspension. After centrifugation, the supernatant was taken as the enzyme solution to be tested. Another 1% soluble starch is used as a substrate.
  • Example 1 The prepared oligomeric proanthocyanidins (Example 10PC) and commercially available grape seed extract (Grape seed 0PC) containing more than 60% of 0PC were made 0.1% aqueous solution, and Example 3 was prepared to obtain Pinus massoniana condensation. 1% ⁇ The tannin extract was also formulated into a 0.1% aqueous solution.
  • test tube was added with 2 ml of substrate, 1 ml of enzyme solution, and 0, 0.02%, 0.04%, 0.06%, 0.08%, 0.1% of oligomeric proanthocyanidins (each of which is an example 10PC or Grape seed 0PC) or Example 3 prepared a solution of Pinus massoniana condensed tannin extract, which was separately mixed and then incubated in a 37 ° C water bath for 60 minutes.
  • a 1 ml sample was taken at 0 hours and 1 hour, and immediately heated in a 100 Torr water bath for 30 minutes to inactivate the enzyme, cooled, and the reducing sugar content was determined by 3,5-dinitrosalicylic acid colorimetric method.
  • the protein content was determined by Coomassie Brilliant G250 colorimetric method.
  • Amylase activity (IU) was calculated as: micromolar reducing sugar / microgram protein. min.
  • the experimental data is shown in Table 2.
  • mice 80 animals were tested and broiler chickens (chicks) were isolated from the 11-day-old weighing group, 8 rats in each group, randomized, the body weight was similar, the error between groups was ⁇ 10 g, the stool was checked before the test, and the infection was confirmed. Coccidia chickens were used as test chickens.
  • the sample to be tested was evenly mixed with the feed. The drug-containing material was fed 24 hours before inoculation, and fed for 7 days. The sample to be tested was 130 ppm of the oligomeric proanthocyanidins and salinomycin prepared in Example 1 respectively.
  • Eimeria tenella was inoculated, except for the non-attack group, each of the other groups was infected with 10 X 10 4 sporulated oocysts.
  • the spirit, appetite and feces were examined daily after infection, and feces were collected on the 7th day, oocysts were counted, and culling was performed on the 8th day to observe the cecal lesions.
  • the experimental data are shown in Table 3, and the production performance is shown in Table 4.
  • Example 8 Therapeutic effect of oligomeric proanthocyanidins on chicken coccidiosis
  • Test animals AA broiler chickens (chickens) 190 pigeons, isolation and flattening to 11-day-old weighing randomized group, 10 rats in each group, the error between groups was ⁇ 10 g, check the feces before the test, and determine that the uninfected coccidia chicken was used as the experimental chicken. .
  • the sample to be tested (see Table 5) was evenly mixed with the feed, and the medicated feed was started 24 hours before the inoculation, and fed continuously for 7 days. At 12 days of age, Eimeria tenella was inoculated (reinforced by chicken before use). Except for the non-attack group, each group of chickens was infected with 10 X 10 4 sporulated oocysts. Check mental, appetite and fecal changes daily after infection, collect feces on day 7 and perform oocyst counts,
  • Grape seed 0PC contains more than 60% oligomeric proanthocyanidins; pine bark 0PC contains about 30% oligomerization Protoplast anthocyanins, which are commercially available products.
  • the oligomeric proanthocyanidin group (Example 1 0PC, Grape Seed 0PC) was better than salinomycin, sea 'Nanthomycin, in bloody stool score, lesion value, oocyst value, and anticoccidial index (ACI). The effect of the maduramycin group was good.
  • the oligomeric proanthocyanidin group and nicarbazin (62. 5ppm) were combined with the anticoccidial index close to the total addition of nicarbazin (125ppm).
  • the daily weight gain of the oligomeric proanthocyanidin group was better than that of salinomycin, Hainanmycin, Maduramycin, and Sedummycin.
  • the oligomeric proanthocyanidins and nicarbazin (62. 5ppm) compounded daily weight gain rate close to the full addition of nicarbazin (125ppm).
  • Example 9 Therapeutic effect of oligomeric proanthocyanidins and Vc or bromelain on chicken coccidiosis test Animals AA broiler (chick) 140 pigeons, isolated and raised to 11 days old weighing random group, each group of 10, group The error was ⁇ 10 g, and the feces were checked before the test, and the chickens that were not infected with coccidia were determined as test chickens. The sample to be tested (see Table 7) was evenly mixed with the feed, and the medicated feed was started 24 hours before the inoculation, and fed continuously for 7 days. At 12 days of age, Eimeria tenella was inoculated (reinforced by chicken before use).
  • Grape seed 0PC contains more than 60% oligomeric proanthocyanidins.

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
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Abstract

La présente invention concerne un additif pour nourriture, le procédé de préparation de celui-ci et l'application de celui-ci. L'additif pour nourriture est constitué de proanthocyanidines oligomériques, les proanthocyanidines oligomériques étant des bipolymères, des terpolymères ou des tétrapolymères. Le procédé de préparation de l'additif pour nourriture consiste à : utiliser du tanin condensé ou un extrait ayant du tanin condensé comme constituant et 20-60 % de peroxyde d'hydrogène ou de chlorate de potassium en tant qu'oxydant, effectuer une réaction d'oxydation pendant 15-120 minutes dans des conditions de pH 6-7 et de température de 30-50°C pour produire un liquide presque transparent ayant une couleur jaune à brune qui est ensuite séparé et purifié pour obtenir l'additif pour nourriture. L'additif pour nourriture peut être utilisé dans une nourriture pour prévenir et traiter la coccidiose animale et sa concentration dans la nourriture est de 5-600 ppm. L'additif pour nourriture peut être utilisé dans la nourriture pour des poulets, des canards, des cailles, des pigeons, des lapins et des porcs. Il peut également être utilisé en même temps que d'autres médicaments contre la coccidiose, qu'un antioxydant ou qu'une préparation d'enzymes.
PCT/CN2005/000960 2005-06-30 2005-06-30 Additif pour nourriture, procédé de préparation de celui-ci et application de celui-ci WO2007003068A1 (fr)

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Cited By (11)

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US20120329736A1 (en) * 2011-06-24 2012-12-27 Huang Alexander L Treatment for gastrointestinal disorders using a selective, site-activated binding system
US20120328711A1 (en) * 2011-06-24 2012-12-27 Huang Alexander L Antimicrobial system for selective, site-activated binding at target sites
US20120328710A1 (en) * 2011-06-24 2012-12-27 Huang Alexander L Selective, site-activated binding of toxins at target sites
CN103250916A (zh) * 2013-06-08 2013-08-21 潘李亮 一种40～60日龄獭兔专用饲料添加剂
US8586110B2 (en) 2007-12-28 2013-11-19 Liveleaf, Inc. Therapeutic composition produced using Camellia sinensis leaves and hydrogen peroxide
US8716352B1 (en) 2012-12-23 2014-05-06 Liveleaf, Inc. Tannin formulation for treating GI spasms
US8722040B2 (en) 2011-06-24 2014-05-13 Liveleaf, Inc. Site-activated binding systems that selectively increase the bioactivity of phenolic compounds at target sites
US8734867B2 (en) 2007-12-28 2014-05-27 Liveleaf, Inc. Antibacterial having an extract of pomegranate combined with hydrogen peroxide
CN109601709A (zh) * 2019-01-04 2019-04-12 湖北建始五节酒业有限公司 一种提高动物免疫性能的饲料添加剂、其制备方法及应用
CN114176157A (zh) * 2021-11-15 2022-03-15 安佑生物科技集团股份有限公司 一种抑制畜禽球虫的绿色添加剂、制备方法及应用
CN114794312A (zh) * 2022-04-24 2022-07-29 广州市正百饲料科技有限公司 核苷酸小肽鞣酸蛋白饲料添加剂及其制备方法和应用

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4797421A (en) * 1984-07-03 1989-01-10 Kikkoman Corporation Antioxidant comprising proanthocyanidin as principal component
US5804597A (en) * 1996-06-10 1998-09-08 Kikkoman Corporation Agent for the prevention or treatment of cataracts
JP2000044472A (ja) * 1998-07-30 2000-02-15 Kikkoman Corp 糖尿病性合併症の予防または治療薬剤
CN1356994A (zh) * 1999-04-23 2002-07-03 协和发酵工业株式会社 原花色素低聚物的提纯方法
US6420572B1 (en) * 1997-10-09 2002-07-16 Mars, Incorporated Synthetic methods for preparation of protected proanthocyanidin(s)
US20040058878A1 (en) * 2002-01-18 2004-03-25 Walker Edward B. Antimicrobial and sporicidal composition

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4797421A (en) * 1984-07-03 1989-01-10 Kikkoman Corporation Antioxidant comprising proanthocyanidin as principal component
US5804597A (en) * 1996-06-10 1998-09-08 Kikkoman Corporation Agent for the prevention or treatment of cataracts
US6420572B1 (en) * 1997-10-09 2002-07-16 Mars, Incorporated Synthetic methods for preparation of protected proanthocyanidin(s)
JP2000044472A (ja) * 1998-07-30 2000-02-15 Kikkoman Corp 糖尿病性合併症の予防または治療薬剤
CN1356994A (zh) * 1999-04-23 2002-07-03 协和发酵工业株式会社 原花色素低聚物的提纯方法
US20040058878A1 (en) * 2002-01-18 2004-03-25 Walker Edward B. Antimicrobial and sporicidal composition

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
LISHUANG L.: "Research process of inartificial antioxidant oligomeric proanthocyanidin", FOOD SCIENCE, vol. 23, no. 2, 2002, pages 147 - 150 *

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US9636361B2 (en) 2007-12-28 2017-05-02 Liveleaf, Inc. Method of killing a bacteria with a plant-based biocidal solution
US8734867B2 (en) 2007-12-28 2014-05-27 Liveleaf, Inc. Antibacterial having an extract of pomegranate combined with hydrogen peroxide
US9192635B2 (en) * 2011-06-24 2015-11-24 Liveleaf, Inc. Method of treating damaged mucosal or gastrointestinal tissue by administering a composition comprising a mixture of pomegranate and green tea extracts and releasably bound hydrogen peroxide
US20120329736A1 (en) * 2011-06-24 2012-12-27 Huang Alexander L Treatment for gastrointestinal disorders using a selective, site-activated binding system
US8722040B2 (en) 2011-06-24 2014-05-13 Liveleaf, Inc. Site-activated binding systems that selectively increase the bioactivity of phenolic compounds at target sites
US20120328711A1 (en) * 2011-06-24 2012-12-27 Huang Alexander L Antimicrobial system for selective, site-activated binding at target sites
US20120328710A1 (en) * 2011-06-24 2012-12-27 Huang Alexander L Selective, site-activated binding of toxins at target sites
US9603883B2 (en) 2012-12-23 2017-03-28 Liveleaf, Inc. Methods of inhibiting a bacterial virulence in a subject
US8946304B2 (en) 2012-12-23 2015-02-03 Liveleaf, Inc. Methods of treating malnutrition
US9089596B1 (en) 2012-12-23 2015-07-28 Liveleaf, Inc. Methods of treating drug side-effects that include a gastrointestinal spasm
US8952072B2 (en) 2012-12-23 2015-02-10 Liveleaf, Inc. Tannin formulation for treating malnutrition
US9408869B2 (en) 2012-12-23 2016-08-09 Liveleaf, Inc. Methods of treating a gastrointestinal spasm associated with chemotherapy or radiation therapy
US9603871B2 (en) 2012-12-23 2017-03-28 Liveleaf, Inc. Methods of treating gastroesophageal reflux disease
US8716352B1 (en) 2012-12-23 2014-05-06 Liveleaf, Inc. Tannin formulation for treating GI spasms
US9023895B1 (en) 2012-12-23 2015-05-05 Liveleaf, Inc. Methods of treating necrotic enteritis
US9907818B2 (en) 2012-12-23 2018-03-06 Liveleaf, Inc. Methods of treating a treatment-resistant gastrointestinal spasm
US10039784B2 (en) 2012-12-23 2018-08-07 Liveleaf, Inc. Methods of treating a treatment-resistant gastrointestinal spasm with an oxidized tannin
US10493102B2 (en) 2012-12-23 2019-12-03 Liveleaf, Inc. Methods of inhibiting the virulence of a pathogen with an oxidized tannin to treat a gastrointestinal spasm
CN103250916A (zh) * 2013-06-08 2013-08-21 潘李亮 一种40～60日龄獭兔专用饲料添加剂
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