WO2006106400A2 - Capsules containing seminal material for artificial insemination - Google Patents
Capsules containing seminal material for artificial insemination Download PDFInfo
- Publication number
- WO2006106400A2 WO2006106400A2 PCT/IB2006/000768 IB2006000768W WO2006106400A2 WO 2006106400 A2 WO2006106400 A2 WO 2006106400A2 IB 2006000768 W IB2006000768 W IB 2006000768W WO 2006106400 A2 WO2006106400 A2 WO 2006106400A2
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- capsules
- microcapsules
- seminal material
- cross
- suspension
- Prior art date
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/48—Reproductive organs
- A61K35/52—Sperm; Prostate; Seminal fluid; Leydig cells of testes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D19/00—Instruments or methods for reproduction or fertilisation
- A61D19/02—Instruments or methods for reproduction or fertilisation for artificial insemination
- A61D19/022—Containers for animal semen, e.g. pouches or vials ; Methods or apparatus for treating or handling animal semen containers, e.g. filling or closing
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
- A61K9/50—Microcapsules having a gas, liquid or semi-solid filling; Solid microparticles or pellets surrounded by a distinct coating layer, e.g. coated microspheres, coated drug crystals
- A61K9/5005—Wall or coating material
- A61K9/501—Inorganic compounds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
- A61K9/50—Microcapsules having a gas, liquid or semi-solid filling; Solid microparticles or pellets surrounded by a distinct coating layer, e.g. coated microspheres, coated drug crystals
- A61K9/5005—Wall or coating material
- A61K9/5021—Organic macromolecular compounds
- A61K9/5036—Polysaccharides, e.g. gums, alginate; Cyclodextrin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
- A61K9/50—Microcapsules having a gas, liquid or semi-solid filling; Solid microparticles or pellets surrounded by a distinct coating layer, e.g. coated microspheres, coated drug crystals
- A61K9/5089—Processes
Definitions
- the present invention relates to capsules containing seminal material of animal species chosen from the class consisting of: equids, buffalo, ovicaprids, canids, felids, lagomorphs, laboratory animal species chosen from mice and rats, and possibly man, suitable for artificial insemination and the relative process for preparing said capsules or microcapsules.
- animal species chosen from the class consisting of: equids, buffalo, ovicaprids, canids, felids, lagomorphs, laboratory animal species chosen from mice and rats, and possibly man, suitable for artificial insemination and the relative process for preparing said capsules or microcapsules.
- animal species chosen from the class consisting of: equids, buffalo, ovicaprids, canids, felids, lagomorphs, laboratory animal species chosen from mice and rats, and possibly man, suitable for artificial insemination and the relative process for preparing said capsules or microcapsules.
- Instrumental insemination also known as artificial insemination (Al) in zootechnics, officially tested from the second half of the 1700s, was extensively developed and applied in particular at the beginning of the 1900s.
- the species having had the most recourse to Il is the bovine species, because of its economic importance and also precise physiological controls enable the procedure to be undertaken with considerable precision and high probability of success.
- the procedure mainly comprised the use of fresh and/or refrigerated seminal material.
- Instrumental insemination was undertaken at public or private facilities also using seminal material derived from external collection centres. Over the last few years, Il techniques have considerably improved following the optimisation and wide diffusion of seminal material freezing techniques: cryopreservation and Il have enabled excellent yields in terms of fertilization and fertility to be achieved in the bovine sector.
- Nebel was advantageous in that the artificial insemination method presented some essential requisites, such as low cost, protection of seminal material from external agents, ease of handling by the operators and practicality of use.
- the principal aim was to improve birth rate results and to reduce phagocytosis of the spermatozoa by leucocytes, in the uterus.
- bovine seminal material proved to be highly complex as it comprised three stages:
- the bivalent ions diffuse towards the surface of the drop and, at the interface, cause the alginate to gel with formation of a semi-permeable membrane.
- US patent No 6,596,310 from 2003 gives the preparation of capsules for pig spermatozoa release:
- the described method concerns the preliminary dilution of seminal material in a medium that prevents premature capacitation (a process indispensable for fertilization) and subsequent encapsulation of diluted seminal material.
- premature capacitation a process indispensable for fertilization
- subsequent encapsulation of diluted seminal material At the point of II, to achieve fertilization, it is indispensable to introduce capacitating agents into the uterus together with the capsules. This process is therefore complex and in practice is not easy to use in farming.
- cryopreservation or encapsulation of seminal material have allowed the Il technique to be improved
- the use of Il in equine species is particularly problematic in that stallion seminal material is difficult to cryopreserve: once frozen, equine spermatozoa lose most of their efficiency and reproductive effectiveness.
- the spermatozoa of equine species are particularly sensitive to the addition of diluants; indeed, the response of the spermatozoa to dilution is an initial increase in cellular activity followed by a drastic reduction in motility and substantial structural changes to the plasma membrane.
- capsules or microcapsules containing live and viable seminal material can be produced, without diluting the seminal material, in a single preparative step which preserves the genetic heritage of the male animal.
- Said capsules or microcapsules can be stored either at ambient temperature or under controlled refrigeration after suspending in diluents for seminal material and used for Il operations.
- the present invention therefore provides:
- Capsules or microcapsules comprising: a) a nucleus containing seminal material or the spermatozoa of animal species chosen from the group consisting of: equids, buffalo, ovicaprids, canids, felids, lagomorphs, laboratory animal species chosen from mice and rats, and possibly man, b) a membrane of a bivalent or trivalent metal alginate.
- a particular aspect of the present invention are capsules or microcapsules containing equine seminal material.
- the gradual bioerosion of the capsules or microcapsules in the uterus (which depends on membrane thickness and characteristics) allows high concentrations of spermatozoa to be maintained within the uterus and tubes for a prolonged period of time able to cover the entire duration of oestrus, thus resulting in an increase in fertilization and fertility.
- the use of the encapsulated or microencapsulated seminal material of the present patent application allows a single Il operation to be undertaken, to considerably reduce the number of operations in that the living and viable spermatozoa are released in a technically predetermined time period which covers the entire ovulation period.
- a further aspect of the present invention is the process for preparing the aforesaid capsules or microcapsules which in particular comprise the following steps:
- step 2) the suspension from step 1) is extruded drop by drop through single or multiple needles or nozzles; the extruded drops are collected in a sodium alginate solution to obtain capsules or microcapsules of a gelatinous type which are separated by filtration and subsequently dispersed in a suitable diluent for encapsulated seminal material;
- the capsules or microcapsules obtained in the preceding step may possibly be cross-linked on the internal and/or external surface, by relative suspension in an aqueous solution of a polyamine type cross-linking agent maintained under agitation, to give rise to rigid microcapsules or capsules.
- capsules of gelatinous appearance are obtained in a single step, without dilution of the seminal material, then filtered off and washed; they can be suspended in a diluting medium, with no capacitating agent, and then used for Il in equine species.
- the described and claimed process for forming the capsules allows the morphological, functional and genetic characteristics of the contained seminal material to remain unchanged in that the membrane protects the spermatozoa contained within from external agents and from phagocytosis.
- the capsules or microcapsules of the present invention preferably contain undiluted equine seminal material.
- the aforesaid equine seminal material generally consists of spermatozoa at different stages of development.
- the seminal material can be derived from the ejaculate or withdrawn from various parts of the male genital apparatus by means of techniques known to every person skilled in the art.
- the concentration of spermatozoa in the total seminal material can be determined by direct count using a Makler camera or a B ⁇ rker camera or a cytofluorimeter or semi-automatic and automatic cell counters, and the morphological characteristics
- the nucleus (a) in addition to containing the seminal material can possibly contain a hydrophilic polymer capable of further modulating seminal fluid release at determined times.
- the polymeric material is preferably chosen from the group consisting of: glucans, scleroglucans, mannans, galactomannans, gellans, carrageenans, pectins, polyanhydrides, polyamino acids, polyamines, xanthans, cellulose and derivatives thereof, carboxymethylcellulose, ethylcellulose, methylcellulose, hydroxypropyl cellulose, hydroxypropyl methylcellulose, polyvinyl alcohols, carboxyvinyl polymers, starches, collagens, chitins, chitosans, alginic acid, hyaluronic acid.
- This hydrophilic polymer preferably constitutes between 5% and 60% by weight on the total weight of the microcapsule.
- the constituent bivalent or trivalent metal alginates of the membrane (b) are preferably chosen from those of calcium, barium, strontium, zinc and trivalents from those of aluminium, iron and chromium.
- the capsule or microcapsule membrane of the present invention is of barium alginate.
- the bivalent or trivalent metal alginate to be used for preparing the membrane (b) forms between 0.5% and 50% of the total capsule weight.
- the capsules or microcapsules are generally of spheroid shape with dimensions between 0.5 and 20 mm, preferably between 1 and 10 mm.
- the thickness of the relative membranes is generally between 0.1 and 5 mm, preferably between 0.1 and 3 mm, even more preferably between 0.2 and 1.5 mm.
- said thickness is between 0.4 and 1 mm.
- the weight of the produced capsules is generally between 5 mg and 200 mg, preferably between 20 mg and 100 mg.
- the capsules or microcapsules suspended in the medium can be stored either at ambient temperature or under controlled refrigeration.
- Hormones and biologically active substances such as agents for stimulating maturation and preserving the activity of said equine seminal material can be carried within said capsules.
- the process for preparing the capsules or microcapsules of the present invention is conducted in accordance with the following operating conditions.
- the bivalent metal ion in the form of chloride or sulfate in solution is added to the seminal material suspension of step (1) until a cation concentration of between 1.0 and 1000 mmol/L, preferably between 1 and 500 mmol/L, is obtained.
- a saturated barium chloride solution is added until the concentration of barium ion is preferably between 5.0 and 250 mmol/L, even more preferably between 5.0 and 100 mmol/L.
- Steps (1) and (2) of the process of the present invention are preferably conducted at a temperature between 5 and 40°C, even more preferably at a temperature between 20 and 30 0 C.
- step (2) the suspension of equine seminal material is then extruded through extruders, orifices, nozzles or needles, of dimensions between 50 ⁇ m and
- 50,000 ⁇ m preferably through needles with an internal diameter preferably between 300 ⁇ m and 20,000 ⁇ m, into a stirred sodium alginate solution maintained between 10 and 200 rpm, but more preferably between 20 and 100 rpm.
- the extrusion takes place by means of automatic or semi-automatic micro- encapsulators, peristaltic pumps of piston or reciprocating type, or with a syringe operated manually or by a suitable system, at a rate such as to produce between
- the ratio of volume of extruded cell suspension to alginate solution can be between 1 :1 and 1:1250, preferably between 1 :15 and 1 :50.
- the sodium alginate used in step (2) of the process of the present invention preferably presents, in a 2% aqueous solution, a viscosity between 200 cP and
- the sodium alginate solution presents a concentration of between 0.01 and 5% w/v, but preferably between 0.1 and 1.0% w/v.
- said capsules can be subjected to cross-linking by means of interface polymerisation of the alginate by using cross- linking agents of polyamine type such as: protamine sulfate or phosphate, preferably in the form of a solution at concentrations between 0.01 and 5% w/v, or poly-L-lysine hydrobromide of molecular weight between 1 ,000 and 800,000 in aqueous solution at a concentration preferably between 0.01 and 5% w/v, or polyvinylamine at a concentration between 0.01 and 5% w/v, or chitosans of molecular weight between 15,000 and 1 ,000,000 at concentrations between 0.01 and 5% w/v.
- polyamine type such as: protamine sulfate or phosphate, preferably in the form of a solution at concentrations between 0.01 and 5% w/v, or poly-L-lysine hydrobromide of molecular weight between 1 ,000 and 800,000 in aqueous solution at
- the cross-linking reaction is conducted at a temperature between 5 and 4O 0 C, preferably at 25°C for a time period between 1 minute and 120 minutes, preferably between 3 and 30 minutes.
- the capsules are recovered by filtration, then washed and suspended in a suitable maintenance medium (diluent) known to the expert of the art.
- Example 1 encapsulation of equine seminal material to obtain immediate release capsules 1a) Preparation of seminal material
- a saturated barium chloride solution is added to the ejaculate, after filtering, until the concentration of the barium ion is 5 mmol/L.
- the resulting suspension of seminal material is extruded through needles (26 G x Y 2 ", 0.45 x 13 mm) into a sodium alginate solution of medium viscosity (3500 cP) at 0.5 % w/v in a culture medium consisting of an aqueous solution containing 60 g/l of glucose and 1.2 g/l of sodium bicarbonate; the solution has a pH of 7.4.
- the ratio of seminal material to sodium alginate solution is 1:25.
- the extrusion takes place drop by drop by means of a syringe, at a temperature of 25 0 C.
- the barium ions react with the sodium alginate to form in 30 minutes a membrane of barium alginate at the interface of the single extrusion drops.
- Capsules are obtained which are collected by filtration, washed twice in a diluant for equine semen and suspended in an aliquot thereof.
- Diameter of nucleus 5.0 mm
- the encapsulated seminal material is stored at a temperature of 17°C for 72 hours.
- the viability of the encapsulated spermatozoa determined every 24 hours by the vital stain Trypan Blue, is given hereinafter (on at least 200 cells per specimen):
- the viability of the encapsulated spermatozoa is significantly higher than that of the diluted spermatozoa (see reference example 3)
- Example 2 encapsulation of equine seminal material to obtain prolonged release capsules
- a saturated barium chloride solution is added to the ejaculate until the barium ion concentration is 15 mmol/L.
- the resulting suspension is extruded through needles (26 G x V 2 ", 0.45 x 13 mm) into a sodium alginate solution of medium viscosity (3500 cP) at 0.5 % w/v in a culture medium consisting of an aqueous solution containing 60 g/l of glucose and 1.2 g/l of sodium bicarbonate (Sigma
- the extrusion takes place drop by drop by means of a syringe, at a temperature of
- the barium ions react with the sodium alginate to form in 30 minutes a membrane of barium alginate at the interface of the single extrusion drops.
- Capsules are obtained which are collected by filtration, washed twice in culture medium and suspended in an aliquot thereof.
- Diameter of nucleus 5.0 mm
- the encapsulated seminal material is stored at a temperature of 17°C for 72 hours.
- the viability of the encapsulated spermatozoa determined every 24 hours by the vital stain Trypan Blue, is given hereinafter (on at least 200 cells per specimen):
- the viability of the encapsulated spermatozoa is significantly higher than that of the diluted spermatozoa (see reference example 3).
- Reference example 3 dilution of equine seminal material according to the known art
- the ejaculate is diluted in a 1 :10 ratio with a commercially available diluent for equine semen in accordance with techniques known to the expert of the art.
- Trypan Blue is given hereinafter (on at least 200 cells per specimen):
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Abstract
Description
Claims
Priority Applications (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP06727411A EP1868588A2 (en) | 2005-04-04 | 2006-04-03 | Capsules containing seminal material for artificial insemination |
CA002603689A CA2603689A1 (en) | 2005-04-04 | 2006-04-03 | Capsules containing seminal material for artificial insemination |
MX2007012346A MX2007012346A (en) | 2005-04-04 | 2006-04-03 | Capsules containing seminal material for artificial insemination. |
US11/887,893 US20090208566A1 (en) | 2005-04-04 | 2006-04-03 | Capsules Containing Seminal Material for Artificial Insemination |
NZ562945A NZ562945A (en) | 2005-04-04 | 2006-04-03 | Capsules containing equid seminal material and metal alginate for artificial insemination |
AU2006232012A AU2006232012A1 (en) | 2005-04-04 | 2006-04-03 | Capsules containing seminal material for artificial insemination |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
IT000552A ITMI20050552A1 (en) | 2005-04-04 | 2005-04-04 | CAPSULES CONTAINING SEMINAL MATERIALS FOR ARTIFICIAL INSEMINATION |
ITMI2005A000552 | 2005-04-04 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2006106400A2 true WO2006106400A2 (en) | 2006-10-12 |
WO2006106400A3 WO2006106400A3 (en) | 2007-03-22 |
Family
ID=37027890
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/IB2006/000768 WO2006106400A2 (en) | 2005-04-04 | 2006-04-03 | Capsules containing seminal material for artificial insemination |
Country Status (8)
Country | Link |
---|---|
US (1) | US20090208566A1 (en) |
EP (1) | EP1868588A2 (en) |
AU (1) | AU2006232012A1 (en) |
CA (1) | CA2603689A1 (en) |
IT (1) | ITMI20050552A1 (en) |
MX (1) | MX2007012346A (en) |
NZ (1) | NZ562945A (en) |
WO (1) | WO2006106400A2 (en) |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2008004890A3 (en) * | 2006-07-04 | 2008-06-12 | Geno | Preservation and controlled delivery/release of spermatozoa |
FR2917291A1 (en) * | 2007-06-14 | 2008-12-19 | Cooperative Bretonne D Insemin | MICROCAPSULES CONTAINING MAMMALIAN SPERMATOZOIDS, INSEMINATION DOSE CONTAINING THEM AND A PORCEDA FOR OBTAINING SAME |
FR2953724A1 (en) * | 2009-12-14 | 2011-06-17 | Genes Diffusion | SWINE SEMINAL MATERIAL COMPOSITION FOR ARTIFICIAL INSEMINATION OF SOWS |
WO2013076232A1 (en) | 2011-11-24 | 2013-05-30 | Spermvital As | Methods for the preparation of hydrogels using lipase enzymes |
CN101487841B (en) * | 2009-02-13 | 2014-06-04 | 深圳市人民医院 | Coating carrier and its use in method for sperm maturity detection and non-invasive mature sperm separation |
CN107854450A (en) * | 2017-12-01 | 2018-03-30 | 江苏省农业科学院 | A kind of preparation method of bacterial virus catenase slow release nanometer particulate |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9320297B2 (en) | 2012-03-22 | 2016-04-26 | Lemniscate Innovations Llc | Spherification/reverse spherification automated and integrated system and method |
FR3021522A1 (en) * | 2014-05-28 | 2015-12-04 | Imv Technologies | STRAW FOR THE PRESERVATION OF A PREDETERMINED DOSE OF LIQUID-BASED SUBSTANCES, IN PARTICULAR DILUTED ANIMAL SEED; DILUTION MEDIUM FOR GIVING SUCH A SUBSTANCE; AND SYSTEM COMPRISING THEM |
CN104496264B (en) * | 2014-12-22 | 2016-04-06 | 江西农业大学 | A kind of preparation method of dry powder organosilicon moisture repellent microcapsules |
CN109044878A (en) * | 2018-10-29 | 2018-12-21 | 扬州大学 | A kind of essence submicron capsule and its preparation and application with emulsification function |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0922451A2 (en) * | 1997-11-28 | 1999-06-16 | Università di Pavia | Microcapsules containing seminal material for artificial insemination in pigs |
-
2005
- 2005-04-04 IT IT000552A patent/ITMI20050552A1/en unknown
-
2006
- 2006-04-03 NZ NZ562945A patent/NZ562945A/en not_active IP Right Cessation
- 2006-04-03 AU AU2006232012A patent/AU2006232012A1/en not_active Abandoned
- 2006-04-03 MX MX2007012346A patent/MX2007012346A/en not_active Application Discontinuation
- 2006-04-03 EP EP06727411A patent/EP1868588A2/en not_active Withdrawn
- 2006-04-03 WO PCT/IB2006/000768 patent/WO2006106400A2/en active Application Filing
- 2006-04-03 CA CA002603689A patent/CA2603689A1/en not_active Abandoned
- 2006-04-03 US US11/887,893 patent/US20090208566A1/en not_active Abandoned
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0922451A2 (en) * | 1997-11-28 | 1999-06-16 | Università di Pavia | Microcapsules containing seminal material for artificial insemination in pigs |
Non-Patent Citations (10)
Title |
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ALEXAKIS T ET AL: "Microencapsulation of DNA within alginate microspheres and crosslinked chitosan membranes for in vivo application." APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY. JAN 1995, vol. 50, no. 1, January 1995 (1995-01), pages 93-106, XP009073422 ISSN: 0273-2289 * |
BALL B A ET AL: "Osmotic tolerance of equine spermatozoa and the effects of soluble cryoprotectants on equine sperm motility, viability, and mitochondrial membrane potential." JOURNAL OF ANDROLOGY. 2001 NOV-DEC, vol. 22, no. 6, November 2001 (2001-11), pages 1061-1069, XP009073400 ISSN: 0196-3635 * |
FAUSTINI MASSIMO ET AL: "Boar spermatozoa encapsulated in barium alginate membranes: a microdensitometric evaluation of some enzymatic activities during storage at 18 degrees C." THERIOGENOLOGY. 1 JAN 2004, vol. 61, no. 1, 1 January 2004 (2004-01-01), pages 173-184, XP002402013 ISSN: 0093-691X * |
SHORE M D ET AL: "Fertility comparison between breeding at 24 hours or at 24 and 48 hours after collection with cooled equine semen." THERIOGENOLOGY. 1 OCT 1998, vol. 50, no. 5, 1 October 1998 (1998-10-01), pages 693-698, XP002402099 ISSN: 0093-691X * |
TORRE M L ET AL: "Barium alginate cell-delivery systems: correlation between technological parameters." INTERNATIONAL JOURNAL OF PHARMACEUTICS. 21 AUG 2002, vol. 242, no. 1-2, 21 August 2002 (2002-08-21), pages 389-391, XP002402015 ISSN: 0378-5173 * |
TORRE M L ET AL: "Boar semen controlled delivery system: analysis of batch seasonal variability." INTERNATIONAL JOURNAL OF PHARMACEUTICS. 21 AUG 2002, vol. 242, no. 1-2, 21 August 2002 (2002-08-21), pages 385-387, XP002402011 ISSN: 0378-5173 * |
TORRE M L ET AL: "Boar semen controlled delivery system: storage and in vitro spermatozoa release" JOURNAL OF CONTROLLED RELEASE, ELSEVIER, AMSTERDAM, NL, vol. 85, no. 1-3, 13 December 2002 (2002-12-13), pages 83-89, XP004397768 ISSN: 0168-3659 * |
TORRE M L ET AL: "Calcium alginate capsules containing a hydrophilic polymer for the encapsulation of swine spermatozoa" S.T.P. PHARMA SCIENCES 1998 FRANCE, vol. 8, no. 4, 1998, pages 233-236, XP009073273 ISSN: 1157-1489 * |
TORRE M L ET AL: "Controlled release of swine semen encapsulated in calcium alginate beads" BIOMATERIALS, ELSEVIER SCIENCE PUBLISHERS BV., BARKING, GB, vol. 21, no. 14, July 2000 (2000-07), pages 1493-1498, XP004199070 ISSN: 0142-9612 * |
VIGO DANIELE ET AL: "Boar semen controlled-delivery system: morphological investigation and in vitro fertilization test." REPRODUCTION, FERTILITY, AND DEVELOPMENT. 2002, vol. 14, no. 5-6, 2002, pages 307-314, XP009073281 ISSN: 1031-3613 * |
Cited By (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2008004890A3 (en) * | 2006-07-04 | 2008-06-12 | Geno | Preservation and controlled delivery/release of spermatozoa |
US8178130B2 (en) | 2006-07-04 | 2012-05-15 | Elisabeth Kommisrud | Preservation and controlled delivery / release of spermatozoa |
FR2917291A1 (en) * | 2007-06-14 | 2008-12-19 | Cooperative Bretonne D Insemin | MICROCAPSULES CONTAINING MAMMALIAN SPERMATOZOIDS, INSEMINATION DOSE CONTAINING THEM AND A PORCEDA FOR OBTAINING SAME |
WO2008155285A1 (en) * | 2007-06-14 | 2008-12-24 | Cooperative Bretonne D'insemination Artificielle Porcine | Microcapsules containing mammals' spermatozoids, insemination dose containing same and method for obtaining same |
CN101487841B (en) * | 2009-02-13 | 2014-06-04 | 深圳市人民医院 | Coating carrier and its use in method for sperm maturity detection and non-invasive mature sperm separation |
FR2953724A1 (en) * | 2009-12-14 | 2011-06-17 | Genes Diffusion | SWINE SEMINAL MATERIAL COMPOSITION FOR ARTIFICIAL INSEMINATION OF SOWS |
WO2011080438A1 (en) * | 2009-12-14 | 2011-07-07 | Genes Diffusion | Porcine seminal material composition for artificial insemination of sows |
WO2013076232A1 (en) | 2011-11-24 | 2013-05-30 | Spermvital As | Methods for the preparation of hydrogels using lipase enzymes |
US9578871B2 (en) | 2011-11-24 | 2017-02-28 | Spermvital As | Methods for the preparation of hydrogels using lipase enzymes |
US10041059B2 (en) | 2011-11-24 | 2018-08-07 | Spermvital As | Methods for the preparation of hydrogels |
CN107854450A (en) * | 2017-12-01 | 2018-03-30 | 江苏省农业科学院 | A kind of preparation method of bacterial virus catenase slow release nanometer particulate |
Also Published As
Publication number | Publication date |
---|---|
ITMI20050552A1 (en) | 2006-10-05 |
NZ562945A (en) | 2009-10-30 |
CA2603689A1 (en) | 2006-10-12 |
EP1868588A2 (en) | 2007-12-26 |
WO2006106400A3 (en) | 2007-03-22 |
AU2006232012A1 (en) | 2006-10-12 |
US20090208566A1 (en) | 2009-08-20 |
MX2007012346A (en) | 2008-02-19 |
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