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Hemoglobin overexpression in fungal fermentations

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Publication number
WO2006091094A3
WO2006091094A3 PCT/NL2006/050006 NL2006050006W WO2006091094A3 WO 2006091094 A3 WO2006091094 A3 WO 2006091094A3 NL 2006050006 W NL2006050006 W NL 2006050006W WO 2006091094 A3 WO2006091094 A3 WO 2006091094A3
Authority
WO
Grant status
Application
Patent type
Prior art keywords
host
fungal
oxygen
binding
proteins
Prior art date
Application number
PCT/NL2006/050006
Other languages
French (fr)
Other versions
WO2006091094A2 (en )
Inventor
Vos Willem Meindert De
Peter Jan Punt
Biesebeke Rob Te
Den Hondel Cornelis Antoni Van
Original Assignee
Vos Willem Meindert De
Dsm Ip Assets Bv
Peter Jan Punt
Biesebeke Rob Te
Den Hondel Cornelis Antoni Van
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date

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Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/795Porphyrin- or corrin-ring-containing peptides
    • C07K14/805Haemoglobins; Myoglobins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0055Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10)
    • C12N9/0057Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10) with oxygen as acceptor (1.10.3)
    • C12N9/0061Laccase (1.10.3.2)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411Amylases
    • C12N9/2414Alpha-amylase (3.2.1.1.)
    • C12N9/2417Alpha-amylase (3.2.1.1.) from microbiological source
    • C12N9/242Fungal source
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411Amylases
    • C12N9/2428Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/62Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from Aspergillus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C12P1/02Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using fungi
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GASES [GHG] EMISSION, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels
    • Y02E50/17Grain bio-ethanol

Abstract

The present invention relates to fungal host cells that are transformed with a nucleic acid construct encoding a fungal oxygen-binding proteins or fragments thereof that comprise the oxygen-binding domain. Upon transformation of the host cell with the construct, the oxygen-binding protein confers to the host cell improved fermentation characteristics as compared to untransformed host cells. These characteristics include e.g. increases in oxygen uptake rates, biomass densities, volumetric productivities and/or product yields. The invention further relates to fermentation processes in which the host cells are used and to fungal oxygen binding proteins, in particular fungal flavohemoglobins and hemoglobin domains, and to nucleotide sequences encoding these proteins.
PCT/NL2006/050006 2005-01-12 2006-01-12 Hemoglobin overexpression in fungal fermentations WO2006091094A3 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP05075097 2005-01-12
EP05075097.5 2005-01-12

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP20060700803 EP1841872A2 (en) 2005-01-12 2006-01-12 Hemoglobin overexpression in fungal fermentations
US11795043 US20080193969A1 (en) 2005-01-12 2006-01-12 Hemoglobin Overexpression in Fungal Fermentations

Publications (2)

Publication Number Publication Date
WO2006091094A2 true WO2006091094A2 (en) 2006-08-31
WO2006091094A3 true true WO2006091094A3 (en) 2007-07-12

Family

ID=34937994

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/NL2006/050006 WO2006091094A3 (en) 2005-01-12 2006-01-12 Hemoglobin overexpression in fungal fermentations

Country Status (3)

Country Link
US (1) US20080193969A1 (en)
EP (1) EP1841872A2 (en)
WO (1) WO2006091094A3 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102382863B (en) * 2010-09-03 2015-04-22 中国科学院青岛生物能源与过程研究所 Method for improving exocytosis capacity of engineering bacteria fatty acid
EP2633027B1 (en) * 2010-10-28 2014-06-04 Total Research & Technology Feluy SA Process for polylactic acid production using monascus
CN104140933B (en) * 2014-08-06 2016-06-08 浙江省农业科学院 Aspergillus zrv2011f5 soil and its application

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003250590A (en) * 2001-12-27 2003-09-09 National Institute Of Advanced Industrial & Technology Aspergillus oryzae flh2 gene
EP1384782A1 (en) * 2001-03-30 2004-01-28 National Food Research Institute Method of detecting the expression of aspergillus gene
EP1609867A1 (en) * 2004-06-25 2005-12-28 Feng Chia University Nucleic acid construct, expression vector and method for enhancing the production of recombinant protein

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5908764A (en) * 1997-05-22 1999-06-01 Solidago Ag Methods and compositions for increasing production of erythromycin
US7163819B2 (en) * 2005-04-14 2007-01-16 E. I. Du Pont De Nemours And Company Bacterial hemoglobin genes and their use to increase carotenoid production

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1384782A1 (en) * 2001-03-30 2004-01-28 National Food Research Institute Method of detecting the expression of aspergillus gene
JP2003250590A (en) * 2001-12-27 2003-09-09 National Institute Of Advanced Industrial & Technology Aspergillus oryzae flh2 gene
EP1609867A1 (en) * 2004-06-25 2005-12-28 Feng Chia University Nucleic acid construct, expression vector and method for enhancing the production of recombinant protein

Non-Patent Citations (12)

* Cited by examiner, † Cited by third party
Title
BHAVE SANJAY L ET AL: "Expression of Vitreoscilla hemoglobin improves growth and levels of extracellular enzyme in Yarrowia lipolytica.", BIOTECHNOLOGY AND BIOENGINEERING, vol. 84, no. 6, 20 December 2003 (2003-12-20), pages 658 - 666, XP002332564, ISSN: 0006-3592 *
DATABASE EMBL [online] 1 March 2005 (2005-03-01), "Aspergillus oryzae hmb1 gene for putative haemoglobin", XP002403044, retrieved from EBI accession no. EM_PRO:AJ628839 Database accession no. AJ628839 *
DATABASE EMBL [online] 28 July 2004 (2004-07-28), "Aspergillus fumigatus mRNA for putative flavohaemoglobin (fh gene)", XP002332571, retrieved from EBI accession no. EM_FUN:AFU578496 Database accession no. AFU578496 *
DATABASE Geneseq [online] 28 March 2003 (2003-03-28), "Aspergillus oryzae polynucleotide SEQ ID NO 4117.", XP002332570, retrieved from EBI accession no. GSN:ABZ55004 Database accession no. ABZ55004 *
DEMODENA J A ET AL: "The production of cephalosporin C by Acremonium chrysogenum is improved by the intracellular expression of a bacterial hemoglobin", BIO/TECHNOLOGY, NATURE PUBLISHING CO. NEW YORK, US, no. 11, August 1993 (1993-08-01), pages 926 - 929, XP002076831, ISSN: 0733-222X *
FREY ALEXANDER D ET AL: "Bacterial hemoglobins and flavohemoglobins: Versatile proteins and their impact on microbiology and biotechnology.", FEMS MICROBIOLOGY REVIEWS, vol. 27, no. 4, October 2003 (2003-10-01), pages 525 - 545, XP002332565, ISSN: 0168-6445 *
LIN YU-HUI ET AL: "Intracellular expression of Vitreoscilla hemoglobin in Aspergillus terreus to alleviate the effect of a short break in aeration during culture.", BIOTECHNOLOGY LETTERS, vol. 26, no. 13, July 2004 (2004-07-01), pages 1067 - 1072, XP002332563, ISSN: 0141-5492 *
MIZUTANI OSAMU ET AL: "Disordered cell integrity signaling caused by disruption of the kexB gene in Aspergillus oryzae", EUKARYOTIC CELL, vol. 3, no. 4, August 2004 (2004-08-01), pages 1036 - 1048, XP002332567, ISSN: 1535-9778 *
PUNT P J ET AL: "Filamentous fungi as cell factories for heterologous protein production", TRENDS IN BIOTECHNOLOGY, ELSEVIER PUBLICATIONS, CAMBRIDGE, GB, vol. 20, no. 5, 1 May 2002 (2002-05-01), pages 200 - 206, XP004347070, ISSN: 0167-7799 *
TE BIESEBEKE R ET AL: "Different control mechanisms regulate glucoamylase and protease gene transcription in Aspergillus oryzae in solid-state and submerged fermentation.", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. APR 2005, vol. 67, no. 1, April 2005 (2005-04-01), pages 75 - 82, XP002332568, ISSN: 0175-7598 *
TE BIESEBEKE ROB: "Molecular analysis and improvement of protein production by Aspergillus oryzae grown on solid substrates", 29 March 2005, THESIS WAGENINGEN UNIVERSITY, WAGENINGEN, ISBN: 90-8504-144-9, XP002332569 *
ZHU H ET AL: "YEAST FLAVOHEMOGLOBIN IS AN ANCIENT PROTEIN RELATED TO GLOBINS AND A REDUCTASE FAMILY", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 89, no. 11, 1992, pages 5015 - 5019, XP002332566, ISSN: 0027-8424 *

Also Published As

Publication number Publication date Type
US20080193969A1 (en) 2008-08-14 application
WO2006091094A2 (en) 2006-08-31 application
EP1841872A2 (en) 2007-10-10 application

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