WO2006085983A2 - Viral adjuvants - Google Patents
Viral adjuvants Download PDFInfo
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- WO2006085983A2 WO2006085983A2 PCT/US2005/024424 US2005024424W WO2006085983A2 WO 2006085983 A2 WO2006085983 A2 WO 2006085983A2 US 2005024424 W US2005024424 W US 2005024424W WO 2006085983 A2 WO2006085983 A2 WO 2006085983A2
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- alphavirus
- adjuvant
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/39—Medicinal preparations containing antigens or antibodies characterised by the immunostimulating additives, e.g. chemical adjuvants
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/12—Viral antigens
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/12—Viral antigens
- A61K39/145—Orthomyxoviridae, e.g. influenza virus
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
- A61K2039/525—Virus
- A61K2039/5252—Virus inactivated (killed)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
- A61K2039/525—Virus
- A61K2039/5258—Virus-like particles
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/54—Medicinal preparations containing antigens or antibodies characterised by the route of administration
- A61K2039/541—Mucosal route
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/555—Medicinal preparations containing antigens or antibodies characterised by a specific combination antigen/adjuvant
- A61K2039/55511—Organic adjuvants
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/555—Medicinal preparations containing antigens or antibodies characterised by a specific combination antigen/adjuvant
- A61K2039/55511—Organic adjuvants
- A61K2039/55544—Bacterial toxins
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/555—Medicinal preparations containing antigens or antibodies characterised by a specific combination antigen/adjuvant
- A61K2039/55511—Organic adjuvants
- A61K2039/55555—Liposomes; Vesicles, e.g. nanoparticles; Spheres, e.g. nanospheres; Polymers
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/555—Medicinal preparations containing antigens or antibodies characterised by a specific combination antigen/adjuvant
- A61K2039/55511—Organic adjuvants
- A61K2039/55561—CpG containing adjuvants; Oligonucleotide containing adjuvants
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
- C12N2740/15034—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
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- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/16011—Orthomyxoviridae
- C12N2760/16111—Influenzavirus A, i.e. influenza A virus
- C12N2760/16134—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
- C12N2770/00011—Details
- C12N2770/16011—Caliciviridae
- C12N2770/16034—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
- C12N2770/00011—Details
- C12N2770/36011—Togaviridae
- C12N2770/36111—Alphavirus, e.g. Sindbis virus, VEE, EEE, WEE, Semliki
- C12N2770/36132—Use of virus as therapeutic agent, other than vaccine, e.g. as cytolytic agent
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
- C12N2770/00011—Details
- C12N2770/36011—Togaviridae
- C12N2770/36111—Alphavirus, e.g. Sindbis virus, VEE, EEE, WEE, Semliki
- C12N2770/36141—Use of virus, viral particle or viral elements as a vector
- C12N2770/36143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
Definitions
- the present invention relates to adjuvants for enhancing immune responses; in particular, the present invention concerns viral adjuvants for achieving enhanced immune responses.
- Vaccination has long provided the most effective tool in the fight against infectious diseases.
- Many vaccination regimens exist which allow the manipulation of the type of immune response required for protection from a given pathogen.
- the use of adjuvants, or compounds co-administered with antigen which augment antigen-specific immune responses, have proven to be extremely beneficial for the induction of protective immunity.
- Many infectious agents rely on mucosal surfaces for entry into the body. Therefore, adjuvants capable of inducing immune responses and which interfere with the early stages of pathogen entry at mucosal surfaces represent powerful tools in the fight against mucosal infections. Summary of the Invention
- the present invention is based, in part, on the discovery that virus particles can act as adjuvants or immunostimulatory agents to enhance host immune responses (cellular and/or humoral). It has previously been known that recombinant viruses expressing an immunogen can serve as vaccine vectors to deliver the immunogen to a subject to induce an immune response thereto. In contrast, the inventors have surprisingly found that virus particles (e.g., replicating virus particles) can act as an adjuvant to enhance an immune response against an immunogen that is not presented on or expressed by the virus. The adjuvant can enhance mucosal and/or systemic immune responses.
- virus particles e.g., replicating virus particles
- the adjuvant can enhance mucosal and/or systemic immune responses.
- the invention provides a method of producing an immune response against an immunogen in a subject, comprising: (a) administering the immunogen to the subject in an immunogenically effective amount; and
- the invention further provides a method of producing an immune response against an immunogen in a subject, the method comprising:
- VEE propagation-defective Venezuelan Equine Encephalitis
- the invention provides an alphavirus adjuvant comprising: a modified alphavirus genomic nucleic acid that lacks sequences encoding the alphavirus structural proteins required for production of new alphavirus particles; wherein the modified alphavirus genome does not comprise a heterologous nucleic acid sequence that encodes a polypeptide of interest or a functional untranslated RNA.
- the alphavirus adjuvant is self-replicating. (i.e., the modified alphavirus genomic nucleic acid comprises sufficient coding nsP1 to nsP4 gene sequences for the genomic nucleic acid to replicate).
- the alphavirus adjuvant comprises a propagation-defective alphavirus particle that further comprises an alphavirus virion coat that packages the modified alphavirus genomic nucleic acid.
- the viral adjuvant is a VEE virus adjuvant in which the viral adjuvant comprises a virion coat comprising VEE structural proteins and/or the viral adjuvant comprises a modified VEE genomic nucleic acid.
- the VEE virus adjuvant is self-replicating.
- the modified alphavirus genomic nucleic acid is a modified VEE viral genomic nucleic acid.
- the alphavirus adjuvant comprises a propagation-defective VEE particle that further comprises a VEE virion coat that packages the modified alphavirus genomic nucleic acid (e.g., a modified VEE genomic nucleic acid).
- the invention also provides compositions comprising the inventive viral adjuvants, and optionally an immunogen, and pharmaceutical formulations comprising the viral adjuvants or compositions of the invention.
- the present invention provides a helper cell for producing an alphavirus adjuvant comprising an infectious propagation- defective alphavirus particle, comprising in an alphavirus-permissive cell:
- a modified alphavirus genomic RNA that comprises (i) sequences encoding the alphavirus nonstructural proteins, and (ii) a competent alphavirus packaging sequence, and (iii) wherein the modified alphavirus genomic nucleic acid lacks sequences encoding the alphavirus structural proteins required for production of new alphavirus particles; and further wherein the modified alphavirus genomic nucleic acid does not comprise a heterologous nucleic acid sequence that encodes a polypeptide of interest or a functional untranslated RNA;
- the helper nucleic acid(s) are RNA molecules, which are optionally transfected into the helper cell.
- the invention provides a method of making an alphavirus adjuvant comprising infectious propagation-defective alphavirus particles, comprising:
- the viral adjuvant is a virus particle (including a live virus, an attenuated live virus, an inactivated virus), a virus- like particle (i.e., a virion coat without genomic nucleic acid), a modified viral genomic nucleic acid (e.g., a replicating genomic nucleic acid), or an unassembled viral structural protein(s) (e.g., alphavirus E1 , E2 and/or capsid proteins, optionally in a liposomal delivery vehicle), or a nucleic acid encoding any of the foregoing.
- a virus particle including a live virus, an attenuated live virus, an inactivated virus
- a virus- like particle i.e., a virion coat without genomic nucleic acid
- a modified viral genomic nucleic acid e.g., a replicating genomic nucleic acid
- an unassembled viral structural protein(s) e.g., alphavirus E1 , E2 and/or capsid proteins,
- the invention is practiced to enhance the immune response to an attenuated live virus, a killed vaccine, a DNA vaccine, or a subunit protein or peptide vaccine, all of which can have the disadvantage of reduced immunogenicity.
- the viral adjuvant is a replicating entity.
- the invention provides for the use of a viral adjuvant for increasing the immune response against an immunogen. Also encompassed by the invention is the use of a virus for the preparation of an adjuvant formulation. The invention further provides the use of a viral adjuvant for the preparation of a medicament.
- the invention provides methods of administering viral particle adjuvants to induce a mucosal response against the virus particles, against an immunogen expressed by the virus particle, and/or against an immunogen that is administered to the subject but is not expressed by the virus particle.
- the virus particle adjuvant can be administered mucosally or non-mucosally.
- Figure 1 shows optical density (OD) measurements of an ELISA
- Figure 2 shows optical density (OD) measurements of an ELISA (1 :40 dilution) for IgG production by lymphoid cultures of non-mucosal tissue (spleen and serum) from mice mock-inoculated or inoculated with virus replicon particles expressing hemagglutinin (VRP), formalin-inactivated influenza virus (dflu), or a co-inoculation thereof (mix).
- VRP hemagglutinin
- dflu formalin-inactivated influenza virus
- Figure 3 shows optical density .(OD) measurements of an ELISA (1 :40 dilution) for IgG production by lymphoid cultures of gut lamina intestinal (LP AVG) and nasal epithelium (Nasal Epi), representative of mucosal effector tissue, from mice-mock inoculated or inoculated with virus replicon particles expressing hemagglutinin (VRP), formalin-inactivated influenza virus (dflu), or a co-inoculation thereof (mix).
- VRP hemagglutinin
- dflu formalin-inactivated influenza virus
- Figure 4 shows optical density (OD) measurements of an ELISA (1 :40 dilution) for IgG production by lymphoid cultures of gut lamina intestinal (LP AVG) and nasal epithelium (Nasal Epi), representative of mucosal effector tissue, from mice mock-inoculated or inoculated with virus replicon particles expressing hemagglutinin (VRP), formalin-inactivated influenza virus (dflu), or a co-inoculation thereof (mix).
- VRP hemagglutinin
- dflu formalin-inactivated influenza virus
- Figure 5 shows dimeric IgA production in the draining lymph node (DLN) and contralateral lymph node (Contra) from mice mock-inoculated or inoculated with virus replicon particles expressing hemagglutinin (VRP), formalin-inactivated influenza virus (dflu), or a co-inoculation thereof (mix).
- LDN draining lymph node
- Contra contralateral lymph node
- Figure 6 shows systemic HA-specific IgG antibody production in the spleen mice following inoculation with virus replicon particles expressing hemagglutinin (VRP), formalin-inactivated influenza virus (dflu), or a co- inoculation thereof (mix). Reciprocal dilution at which the OD is > 0.2 is shown.
- VRP hemagglutinin
- dflu formalin-inactivated influenza virus
- Mix co- inoculation thereof
- Figure 7 shows systemic HA-specific IgA antibody production in the spleen of mice following inoculation with virus replicon particles expressing hemagglutinin (VRP), formalin-inactivated influenza virus (dflu), or a co- inoculation thereof (mix). Reciprocal dilution at which the OD is > two standard deviations over mock is shown.
- VRP virus replicon particles expressing hemagglutinin
- dflu formalin-inactivated influenza virus
- Mix co- inoculation thereof
- Figure 8 shows mucosal HA-specific IgG antibody production in nasal epithelium of mice following inoculation with virus replicon particles expressing hemagglutinin (VRP), formalin-inactivated influenza virus (dflu), or a co-inoculation thereof (mix). Reciprocal dilution at which the OD is > 0.2 is shown.
- VRP hemagglutinin
- dflu formalin-inactivated influenza virus
- Mix co-inoculation thereof
- Figure 9 shows mucosal HA-specific IgA antibody production in nasal epithelium of mice following inoculation with virus replicon particles expressing hemagglutinin (VRP), formalin-inactivated influenza virus (dflu), or a co-inoculation thereof (mix). Reciprocal dilution at which the OD is > two standard deviations over mock is shown.
- VRP hemagglutinin
- dflu formalin-inactivated influenza virus
- Mix co-inoculation thereof
- Figure 10 shows HA-specific IgG antibody production in DLN of mice following inoculation with virus replicon particles expressing hemagglutinin (VRP), formalin-inactivated influenza virus (dflu), or a co-inoculation thereof (mix). Reciprocal dilution at which the OD is > 0.2 is shown.
- Figure 11 shows HA-specific IgA antibody production in DLN of mice following inoculation with virus replicon particles expressing hemagglutinin (VRP), formalin-inactivated influenza virus (dflu), or a co-inoculation thereof (mix). Reciprocal dilution at which the OD is > two standard deviations over mock is shown.
- Figure 12 shows antigen specific, dimeric IgA production in the draining lymph node (DLN) of mice mock inoculated or inoculated with virus replicon particles expressing hemagglutinin (VRP), formalin-inactivated influenza virus (dflu), or a co-inoculation thereof (mix).
- DNN draining lymph node
- Figure 13 shows optical density (OD) measurements of an ELISA for HA-specific IgG (1:40 dilution) and IgA (dilution 1 :5) in the draining lymph node (DLN) and contralateral lymph node (Contra) from mice mock-inoculated or inoculated with virus replicon particles expressing hemagglutinin (VRP), formalin-inactivated influenza virus (dflu), or a co-inoculation thereof (mix).
- OD optical density
- FIG 14A Ovalbumin (OVA)-specific antibody (Ab) production following VRP inoculation.
- Ovalbumin (OVA) Ovalbumin-specific antibody (Ab) production following VRP inoculation.
- Groups of eight Balb/c animals were vaccinated and boosted four weeks later with 10 ⁇ g of Ovalbumin (OVA), or 10 ⁇ g OVA co-inoculated with 1 x 10 infectious units (I. U.) of empty (not expressing any antigen) VRP, either in the footpad (FP) or intranasal (IN).
- FP footpad
- IN intranasal
- Three weeks post boost animals were bled and sera analyzed for the presence of OVA-specific IgG antibodies via ELISA.
- Antibody titers are presented as the reciprocal dilution which results in an optical density (OD) >0.2.
- FIG 14B Ovalbumin (OVA)-specific antibody (Ab) production following VRP inoculation.
- Ovalbumin (OVA) Ovalbumin-specific antibody (Ab) production following VRP inoculation.
- Groups of eight Balb/c animals were vaccinated and boosted four weeks later with 10 ⁇ of Ovalbumin (OVA), or 10 ⁇ g OVA co-inoculated with 1 x 10 6 infectious units (I. U.) of empty (not expressing any antigen) VRP, either in the footpad (FP) or intranasal (IN).
- FP footpad
- IN intranasal
- fecal extracts were prepared from immunized animals and analyzed for the presence of OVA-specific IgG antibodies via ELISA.
- Antibody titers are presented as the reciprocal dilution which results in an optical density (OD) >0.2.
- FIG 14C Ovalbumin (OVA)-specific antibody (Ab) production following VRP inoculation.
- Ovalbumin (OVA) Ovalbumin-specific antibody (Ab) production following VRP inoculation.
- Groups of eight Balb/c animals were vaccinated and boosted four weeks later with 10 ⁇ g of Ovalbumin (OVA), or 10 ⁇ g OVA ft co-inoculated with 1 x 10 infectious units (I. U.) of empty (not expressing any antigen) VRP, either in the footpad (FP) or intranasal (IN).
- FP footpad
- IN intranasal
- Three weeks post boost, fecal extracts were prepared from immunized animals and analyzed for the presence of OVA-specific IgA antibodies via ELISA.
- Antibody titers are presented as the reciprocal dilution which results in an optical density (OD) >0.2.
- FIG. 15A Ovalbumin (OVA)-specific antibody (Ab) production ' "following VRP inoculation.
- Ovalbumin (OVA)-specific antibody (Ab) production ' "following VRP inoculation.
- Groups of eight Balb/c animals were vaccinated and boosted four weeks later in the footpad with PBS, 10 ⁇ g of Ovalbumin (OVA), 10 ⁇ g OVA co-inoculated with 1 x 10 6 infectious units (I.U.) of empty (not expressing any antigen) VRP, 10 ⁇ g OVA co-inoculated with an equivalent dilution of the supernatant from a mock electroporation (mE), 10 ⁇ g OVA co-inoculated with 1 ⁇ g of cholera toxin (CT), or 10 ⁇ g of OVA co- inoculated with 1 x 10 I.U.
- mE mock electroporation
- CT cholera toxin
- UV-treated empty VRP Three weeks post boost, fecal extracts were prepared from immunized animals and analyzed for the presence of OVA-specific IgG antibodies via ELISA. Antibody titers are presented as the reciprocal dilution which results in an optical density (OD) >0.2.
- FIG. 15B OVA-specific antibody (Ab) production following VRP inoculation.
- Groups of eight Balb/c animals were vaccinated and boosted four weeks later in the footpad with PBS 1 10 ⁇ g of Ovalbumin (OVA), 10 ⁇ g OVA co-inoculated with 1 x 10 6 infectious units (I.U.) of empty (not expressing any antigen) VRP, 10 ⁇ g OVA co-inoculated with an equivalent dilution of the supernatant from a mock electroporation (mE), 10 ⁇ g OVA co-inoculated with 1 ⁇ g of cholera toxin (CT) 1 or 10 ⁇ g of OVA co-inoculated with 1 x 10 6 I.U.
- OVA-specific antibody (Ab) production following VRP inoculation Groups of eight Balb/c animals were vaccinated and boosted four weeks later in the footpad with PBS 1 10 ⁇ g of Ovalbumin (OVA), 10 ⁇
- UV- treated empty VRP Three weeks post boost, fecal extracts were prepared from immunized animals and analyzed for the presence of OVA-specific IgG antibodies via ELISA. Antibody titers are presented as the reciprocal dilution which results in an optical density (OD) >0.2.
- Ovalbumin (OVA)-specific antibody (Ab) production following VRP inoculation Groups of eight Balb/c animals were vaccinated and boosted four weeks later in the footpad with PBS, 10 ⁇ g of Ovalbumin (OVA), 10 ⁇ g OVA co-inoculated with 1 x 1.0 2 infectious units (I. U.) of empty (not expressing any antigen) VRP, 1 x 10 3 LU. VRP, 1 x 10 4 LU. VRP, 1 x 10 5 LU. VRP, 1 x 10 6 LU. VRP. Three weeks post boost, animals were bleed and sera analyzed for the presence of OVA-specific IgG antibodies via ELISA. Antibody titers are presented as the reciprocal dilution which results in an optical density (OD) >0.2.
- FIG. 17A Ovalbumin (OVA)-specific antibody (Ab) production following VRP inoculation.
- Ovalbumin (OVA) Ovalbumin-specific antibody (Ab) production following VRP inoculation.
- Groups of six Balb/c animals were vaccinated and boosted four weeks later in the footpad with 10 ⁇ g of Ovalbumin (OVA), 10 ⁇ OVA co-inoculated with 1 ⁇ g of cholera toxin (CT), 10 ⁇ g OVA co-inoculated with 1 x 10 4 infectious units (LU.) of empty (not expressing any antigen) VRP, 10 ⁇ g OVA co-inoculated with 1 x 10 4 LU. empty VRP treated with ultraviolet (UV) light, or 10 ⁇ g of OVA co-inoculated with 1 x 10 6 LU. empty VRP.
- CT cholera toxin
- LU. infectious units
- empty VRP empty
- UV light ultraviolet
- FIG. 17B Ovalbumin (OVA)-specific antibody (Ab) production following VRP inoculation.
- Ovalbumin (OVA) Ovalbumin-specific antibody (Ab) production following VRP inoculation.
- Groups of six Balb/c animals were vaccinated and boosted four weeks later in the footpad with 10 ⁇ g of Ovalbumin (OVA), 10 ⁇ g OVA co-inoculated with 1 ⁇ g of cholera toxin (CT), 10 ⁇ g OVA co-inoculated with 1 x 10 4 infectious units (LU.) of empty (not expressing any antigen) VRP, 10 ⁇ g OVA co-inoculated with 1 x 10 4 LU. empty VRP treated with ultraviolet (UV) light, or 10 ⁇ g of OVA co-inoculated with 1 x 10 6 LU. empty VRP.
- CT cholera toxin
- LU. infectious units
- empty VRP empty
- UV light ultraviolet
- FIG. 17C Ovalbumin (OVA)-specific antibody (Ab) production following VRP inoculation.
- Ovalbumin (OVA) Ovalbumin-specific antibody (Ab) production following VRP inoculation.
- Groups of six Balb/c animals were vaccinated and boosted four weeks later in the footpad withiO ⁇ g of Ovalbumin (OVA), 10 ⁇ g OVA co-inoculated with 1 ⁇ g of cholera toxin (CT), 10 ⁇ g OVA co-inoculated with 1 x 10 4 infectious units (I. U.) of empty (not expressing any antigen) VRP, 10 ⁇ g OVA co-inoculated with 1 x 10 4 I. U. empty VRP treated with ultraviolet (UV) light, or 10 ⁇ g of OVA co-inoculated with 1 x 10 6 LU. empty VRP.
- CT cholera toxin
- I. U. infectious units
- VRP 10 ⁇ g OVA co-ino
- FIG. 1 VRP induction of gp120-specific I FN-g-secreting cells.
- Groups of eight Balb/c animals were vaccinated and boosted four weeks later with PBS, 10 ⁇ g of SIV gp120 protein, 10 ⁇ g of gp120 co-inoculated with 1 x ; 10 6 infectious units (LU.) of empty (not expressing any antigen) VRP, or 1 x 10 6 LU. of VRP expressing full length SIV gp160.
- One week post boost animals were sacrificed and spleens were isolated from immunized animals. Single cell suspensions were prepared from isolated spleens and analyzed for the presence of IFN- ⁇ -secreting cells via IFN- ⁇ ELISPOT following in vitro stimulation with either gp120 peptides or an irrelevant HA peptide. Numbers of IFN- ⁇ -secreting cells are displayed per 5 x 1O 5 CeIIs.
- FIG 19A Norwalk Virus (NV) VLP-specific antibody (Ab) production following VRP inoculation.
- Groups of six Balb/c animals were vaccinated and boosted four weeks later with PBS, 10 ⁇ g of Norwalk virus (NV) virus-like particles (VLP), 10 ⁇ g NV VLP co-inoculated with 1 x 10 6 infectious units (LU.) of empty (not expressing any antigen) VRP, or 10 ⁇ g NV VLP co-inoculated with 1 ⁇ g of cholera toxin (CT).
- fecal extracts were prepared from immunized animals and analyzed for the presence of OVA- specific IgG antibodies via ELISA. Antibody titers are presented as the reciprocal dilution which results in an optical density (OD) >0.2.
- FIG 19B Norwalk Virus (NV) VLP-specific antibody (Ab) production following VRP inoculation.
- Groups of six Balb/c animals were vaccinated and boosted four weeks later with PBS, 10 ⁇ g of Norwalk virus (NV) virus-like particles (VLP), 10 ⁇ g NV VLP co-inoculated with 1 x 10 ⁇ infectious units (I. U.) of empty (not expressing any antigen) VRP, or 10 ⁇ g NV VLP co-inoculated with 1 ⁇ g of cholera toxin (CT).
- CT cholera toxin
- Three weeks post boost fecal extracts were prepared from immunized animals and analyzed for the presence of OVA- specific IgG antibodies via ELISA. Antibody titers are presented as the reciprocal dilution which results in an optical density (OD) >0.2.
- FIG 19C Norwalk Virus (NV) VLP-specific antibody (Ab) production following VRP inoculation.
- Groups of six Balb/c animals were vaccinated and boosted four weeks later with PBS, 10 ⁇ g of Norwalk virus (NV) virus-like ⁇ particles (VLP), 10 ⁇ g NV VLP co-inoculated with 1 x 10 6 infectious units (I. U.) of empty (not expressing any antigen) VRP, or 10 ⁇ g NV VLP co- inoculated with 1 ⁇ g of cholera toxin (CT).
- CT cholera toxin
- Three weeks post boost fecal extracts were prepared from immunized animals and analyzed for the presence of OVA-specific IgA antibodies via ELISA. Antibody titers are presented as the reciprocal dilution which results in an optical density (OD) >0.2.
- the present inventors have made the surprising discovery that virus particles can act as potent adjuvants to enhance an immune response, which effect is unrelated to their ability to act as vaccine vectors.
- the viral adjuvants of the invention can stimulate and enhance an immune response against an immunogen that is independent of the viral adjuvant, i.e., is not presented by the viral adjuvant or expressed by the viral adjuvant.
- the inventors have further made the unexpected discovery that administration of the viral adjuvants of the invention by a non-mucosal route can produce a mucosal immune response against an immunogen that is not presented by or expressed by the viral adjuvant.
- an adjuvant has its ordinary meaning as understood by those in the art.
- an adjuvant can be defined as a substance that increases the ability of an immunogen (i.e., antigen) to stimulate an immune response against the immunogen in the subject.
- the adjuvant increases the immune response against the immunogen by at least about 2, 3, 4, 5, 10, 15, 20, 30, 40, 50, 60, 75, 100, 150, 500, 1000-fold or more.
- the adjuvant reduces the amount of immunogen required to achieve a particular level of immune response (cellular and/or humoral and/or mucosal), e.g., a reduction of at least about 15%, 25%, 35%, 50%, 65%, 75%, 80%, 85%, 90%, 95%, 98% or more.
- An adjuvant can further be a substance that prolongs the time over which an immune response, optionally protective immune response, is sustained (e.g., by at least about a 2-fold, 3-fold, 5-fold, 10-fold, 20-fold longer time period or more).
- an immune response it is meant an immune response
- a mucosal immune response is accompanied by production of antigen-specific IgA molecules.
- systemic immune response it is meant an immune response (cellular and/or humoral) that is detectable in blood and/or lymphoid tissue (e.g., spleen and lymph nodes).
- polypeptide encompasses both peptides and proteins.
- polypeptide of interest is a polypeptide that is desirably introduced and/or expressed in a subject, e.g., because of its biological and/or antigenic properties and includes reporter polypeptides, therapeutic polypeptides, enzymes, growth factors, immunomodulatory polypeptides, and immunogenic polypeptides.
- an "isolated" nucleic acid means a nucleic acid separated or substantially free from at least some of the other components of the naturally occurring organism or virus, for example, the cell or viral structural components or other polypeptides or nucleic acids commonly found associated with the nucleic acid.
- an "isolated" polypeptide means a polypeptide that is separated or substantially free from at least some of the other components of the naturally occurring organism or virus, for example, the cell or viral structural components or other polypeptides or nucleic acids commonly found associated with the polypeptide.
- nucleic acid encompasses both RNA and DNA, including cDNA, genomic DNA, synthetic (e.g., chemically synthesized) DNA and chimeras of RNA and DNA.
- the nucleic acid may be double- stranded or single-stranded. Where single-stranded, the nucleic acid may be a sense strand or an antisense strand.
- the nucleic acid may be synthesized using oligonucleotide analogs or derivatives (e.g., inosine or phosphorothioate nucleotides). Such oligonucleotides can be used, for example, to prepare nucleic acids that have altered base-pairing abilities or increased resistance to nucleases.
- heterologous nucleic acid is a well-known term of art and would be readily understood by one of skill in the art to be a nucleic acid that is foreign to the nucleic acid carrier (e.g., viral or plasmid delivery vector).
- nucleic acid carrier e.g., viral or plasmid delivery vector
- a "functional untranslated RNA” includes, for example, interfering RNA (e.g., siRNA) or antisense RNA.
- heterologous nucleic acid can be associated with appropriate expression control sequences, e.g., transcription/translation control signals and polyadenylation signals.
- the promoter can be constitutive or inducible (e.g., the metalothionein promoter or a hormone inducible promoter), depending on the pattern of expression desired.
- the promoter can be native or foreign and can be a natural or a synthetic sequence. By foreign, it is intended that the promoter is not found in the virus into which the promoter is introduced.
- the promoter is generally chosen so that it will function in the target cell(s) of interest.
- the heterologous nucleotide sequence is operably associated with a promoter that provides high level expression of the heterologous nucleotide sequence, e.g., an alphavirus subgenomic 26S promoter (preferably, a VEE, Sindbis, Girdwood or TR339 26S subgenomic promoter).
- a promoter that provides high level expression of the heterologous nucleotide sequence
- an alphavirus subgenomic 26S promoter preferably, a VEE, Sindbis, Girdwood or TR339 26S subgenomic promoter.
- Inducible expression control elements can be used in those applications in which it is desirable to provide regulation over expression of the heterologous nucleic acid sequence.
- Inducible promoters/enhancer elements include tissue-specific promoter/enhancer elements, which further includes, but is not limited to, muscle specific (including cardiac, skeletal and/or smooth muscle), neural tissue specific (including brain-specific), eye (including retina-specific and cornea-specific), liver specific, bone marrow specific, pancreatic specific, spleen specific, and lung specific promoter/enhancer elements.
- inducible promoter/enhancer elements include hormone-inducible and metal-inducible elements, examples of which include but are not limited to a Tet on/off element, a RU486-inducible promoter, an ecdysone-inducible promoter, a rapamycin-inducible promoter, and a metalothionein promoter.
- specific initiation signals are generally required for efficient translation of inserted polypeptide coding sequences.
- These translational control sequences which can include the ATG initiation codon and adjacent sequences, can be of a variety of origins, both natural and synthetic.
- the transcriptional units can be operatively associated with separate promoters or with a single upstream promoter and one or more downstream internal ribosome entry site (IRES) sequences (e.g., the picomavirus EMC IRES sequence).
- IRES internal ribosome entry site
- specific initiation signals are generally required for efficient translation of inserted protein coding sequences.
- exogenous translational control sequences . which may include the ATG initiation codon and adjacent sequences, can be of a variety of origins, both natural and synthetic.
- RNA or DNA indicates that the nucleic acid sequence is transcribed and, optionally, translated.
- a nucleic acid sequence may express a polypeptide of interest or a functional untranslated RNA.
- treat By the terms “treat,” “treats,” “treating” or “treatment of,” and the like, it is intended that the severity of the patient's condition is reduced or at least partially improved or modified and that some alleviation, mitigation or decrease in at least one clinical symptom is achieved.
- the terms “treat,” “treats,” “treating,” or “treatment of also include prophylactic treatment of the subject to prevent the onset of infection, cancer, transplant rejection, or other symptoms.
- the terms "prevent,” “prevents,” and “prevention” are not meant to imply complete abolition of infection, cancer, transplant rejection or other symptoms and encompasses any type of prophylactic treatment that reduces the incidence of the condition, delays the onset and/or progression of the condition, and/or reduces the symptoms associated with the condition.
- Th ⁇ term “alphavirus” has its conventional meaning in the art, and includes Eastern Equine Encephalitis virus (EEE), Venezuelan Equine Encephalitis virus (VEE), Everglades virus, Mucambo virus, Pixuna virus, Western Encephalitis virus (WEE), Sindbis virus, South African Arbovirus No. 86 (S.A.AR86), Girdwood S.A. virus, Ockelbo virus, Semliki Forest virus,
- Middelburg virus Chikungunya virus, O'Nyong-Nyong virus, Ross River virus, Barmah Forest virus, Getah virus, Sagiyama virus, Bebaru virus, Mayaro virus, Una virus, Aura virus, Whataroa virus, Babanki virus, Kyzlagach virus, Highlands J virus, Fort Morgan virus, Ndumu virus, Buggy Creek virus, and any other virus classified by the International Committee on Taxonomy of Viruses (ICTV) as an alphavirus.
- ICTV International Committee on Taxonomy of Viruses
- Sindbis virus strains e.g., TR339
- VEE e.g., VEE
- S.A.AR86 virus Girdwood S.A: virus
- Ockelbo virus e.g., Sindbis virus strains
- the complete genomic sequences, as well as the sequences of the- various structural and non-structural proteins are known in the art for numerous alphaviruses and include without limitation: Sindbis virus genomic sequence (GenBank Accession Nos. J02363, NCBI Accession No. NC_001547), S.A.AR86 genomic sequence (GenBank Accession No. U38305), VEE genomic sequence (GenBank Accession No. L04653, NCBI Accession No.
- flavivirus has its conventional meaning in the art, and includes tick-borne encephalitis virus, Central European Encephalitis virus, Far Eastern Encephalitis virus, Kunjin virus, Murray Valley Encephalitis virus, St.
- Boyaviridae has its conventional meaning in the art, and includes viruses within the genera Bunyavirus, Hantavirus, Nairovirus and Phlebovirus including but not limited to Bunyamwera virus, Rift Valley Fever virus, La Crosse virus and any other virus classified by the International Committee on Taxonomy of Viruses (ICTV) as a bunyavirus. If the adjuvant virus is a bunyavirus, in particular embodiments, the viral adjuvant comprises only one or two of the three viral RNA segments.
- rotavirus has its conventional meaning in the art, and includes Group A, Group B, Group C, Group D, Group E, Group F and Group G rotaviruses and any other virus classified by the International Committee on Taxonomy of Viruses (ICTV) as a rotavirus.
- ICTV International Committee on Taxonomy of Viruses
- retrovirus has its conventional meaning in the art, and includes the Alpharetrovirus genus (e.g., Avian leucosis virus and Rous sarcoma virus), Betaretrovirus genus (e.g., Mouse mammary tumor virus, Mason-Pfizer monkey virus, Jaagsiekte sheep retrovirus), Gammaretrovirus genus (e.g., Murine leukemia viruses, Feline leukemia virus, Gibbon ape leukemia virus, reticuloendotheliosis virus), Deltaretrovirus genus (e.g., ⁇
- Alpharetrovirus genus e.g., Avian leucosis virus and Rous sarcoma virus
- Betaretrovirus genus e.g., Mouse mammary tumor virus, Mason-Pfizer monkey virus, Jaagsiekte sheep retrovirus
- Gammaretrovirus genus e.g., Murine leukemia viruses, Feline leukemia virus, Gibbon ape leuk
- Human T-lymphotrophic virus Bovine leukemia virus, Simian T-lymphotrophic virus
- Epsilonretrovirus genus e.g., Walleye dermal sarcoma virus, walleye epidermal hyperplasia virus 1
- lentivirus genus e.g., Human immunodeficiency virus [HIV], including HIV-1 and HIV-2, Simian immunodeficiency virus, Equine infectious anemia virus, Feline immunodeficiency virus, Caprine arthritis encephalitis virus, Visna/maedi virus
- the Spumavirus genus e.g., Human foamy virus
- ICTV International Committee on Taxonomy of Viruses
- orthomyxovirus has its conventional meaning in the art, and includes influenza A virus, influenza B virus, influenza C virus, thogotovirus and any other virus classified by the International Committee on Taxonomy of Viruses (ICTV) as an orthomyxovirus.
- ICTV International Committee on Taxonomy of Viruses
- herpesvirus has its conventional meaning in the art, and includes herpes simplex virus I and herpes simplex virus Il and any other virus classified by the International Committee on Taxonomy of Viruses (ICTV) as a herpesvirus.
- non-virus has its conventional meaning in the art, and includes but is not limited to Norwalk virus (including strains Desert Shield virus, Lordsdale virus, Mexico virus, Hawaii virus, Snow Mountain virus, and South Hampton virus) and any other virus classified by the International Committee on Taxonomy of Viruses (ICTV) as a norovirus.
- Norwalk virus including strains Desert Shield virus, Lordsdale virus, Mexico virus, Hawaii virus, Snow Mountain virus, and South Hampton virus
- ICTV International Committee on Taxonomy of Viruses
- non-viral virus has its conventional meaning in the art and refers to viruses in the Nidovirales order, and includes the family Coronoaviridae, which encompasses genera Coronavirus (e.g., avian infectious bronchitis virus, bovine coronavirus, canine coronavirus, feline infectious peritonitis virus, human coronavirus [including strains 229E and OC43], murine hepatitis virus, porcine epidemic diarrhea virus, porcine hemagglutinating encephalomyelitis virus, porcine transmissible gastroenteritis virus, rat coronavirus, turkey coronavirus and rabbit coronavirus) and Torovirus (e.g.
- Coronavirus e.g., avian infectious bronchitis virus, bovine coronavirus, canine coronavirus, feline infectious peritonitis virus, human coronavirus [including strains 229E and OC43], murine hepatitis virus, porcine epidemic diarrhea virus, porcine
- equine torovirus [Berne virus], bovine torovirus [Breda virus], human torovirus and porcine torovirus
- family Arteriviridae e.g., genus Arterivirus including equine arteritis virus, lactate dehydrogenase-elevating virus, porcine respiratory and reproductive syndrome virus, and simian hemorrhagic fever virus
- family Roniviridae e.g., genus Okavirus including gill-associated virus and yellow head virus
- ICTV International Committee on Taxonomy of Viruses
- the term "picomavirus” has its conventional meaning in the art and refers to viruses in the family Picomaviridae and includes viruses in the genera Enterovirus (e.g., bovine enterovirus 1 , bovine enterovirus 2, human enterovirus A [human coxsackievirus A 2, 3, 5, 7, 8, 10, 12, 14 and 16 and human enterovirus 71 strains], human enterovirus B [human coxsackievirus A 9 and B 1 , 2, 3, 4, 5, 6 strains and human echovirus strains], human enterovirus C virus [human coxsachievirus A 1 , 11 , 13, 15, 17, 18, 19, 20, 21 , 22, 24 strains], human enterovirus D [human enterovirus 68 and 70 strains], human enterovirus E, polioviruses [human poliovirus strains], porcine enterovirus A [porcine enterovirus 8], porcine enterovirus B [porcine enterovirus 9 and 10 strains], and simian enterovirus), Rhinovirus (e.g., human
- viral structural protein(s) refers to one or more of the proteins that are constituents of a functional virus particle.
- the alphavirus structural proteins include the capsid protein, E1 glycoprotein, E2 glycoprotein, E3 protein and 6K protein.
- the alphavirus particle comprises the alphavirus structural proteins assembled to form an enveloped nucleocapsid structure.
- alphavirus structural subunits consisting of a single viral protein, capsid, associate with themselves and with the RNA genome to form the icosahedral nucleocapsid, which is then surrounded by a lipid envelope covered with a regular array of transmembranal protein spikes, each of which consists of a heterodimeric complex of two glycoproteins, E1 and E2 (See Paredes et al., (1993) Proc. Natl. Acad. Sci. USA 90, 9095-99; Paredes et al., (1993) Virology 187, 324-32; Pedersen et al., (1974) J. Virol. 14:40).
- a "viral genomic nucleic acid” and similar terms include recombinant and other modified forms (e.g., one or more attenuating mutations, deletions, insertions or otherwise modified viral genomes).
- the viral genomic nucleic acid can be a propagation-incompetent, but replication-competent, replicon as described herein.
- An "alphavirus genomic RNA” indicates the alphavirus RNA transcript, including recombinant and other modified forms.
- the wild-type alphavirus genome is a single-stranded, messenger-sense RNA, modified at the 5'-end with a methylated cap, and at the 3'-end with a variable-length poly (A) tract.
- alphavirus genomic RNA encompasses recombinant alphavirus genomes (e.g., containing a heterologous nucleic acid sequence), viral genomes containing one or more attenuating mutations, deletions, insertions, and/or otherwise modified viral genomes.
- the "alphavirus genomic RNA” may be modified to form a double- promoter molecule or a replicon (each as described herein).
- the viral or alphavirus genomic nucleic acid can optionally comprise a packaging signal (e.g., an alphavirus or VEE packaging signal).
- a "chimeric" virus as used herein comprises structural proteins from one (or more) viruses and a genomic nucleic acid from another virus.
- the chimeric virus is a chimeric alphavirus, e.g., comprising a Sindbis genomic RNA and structural proteins from another alphavirus (e.g., VEE, GirdwoodS.A., Ockelbo, and the like).
- the chimeric alphavirus comprises Sindbis alphavirus structural proteins and a genomic RNA from another alphavirus (e.g., VEE, GirdwoodS.A., Ockelbo, and the like).
- a "chimeric virus” comprises structural proteins and/or nucleic acid from two or more viruses
- a “chimeric alphavirus” comprises structural proteins and/or nucleic acid from two or more alphaviruses (e.g., VEE and Sindbis).
- An "infectious" virus particle is one that can introduce the virus genomic nucleic acid into a permissive cell, typically by viral transduction. Upon introduction into the target cell, the genomic nucleic acid serves as a template for RNA transcription (i.e., gene expression).
- the "infectious" alphavirus particle may be "replication-competent” (i.e., can transcribe and replicate the genomic nucleic acid) and “propagation-competent” (i.e., results in a productive infection in which new virus particles are produced).
- the "infectious" virus particle is a replicon particle that can introduce the genomic nucleic acid (i.e., replicon) into a host cell, is “replication-competent” to replicate the genomic nucleic acid, but is "propagation-defective” or “propagation-incompetent” in that it is unable to produce new virus particles in the absence of helper sequences that complement the deletions or other mutations in the replicon (i.e., provide the structural proteins that are not provided by the replicon).
- a "replicating" or i'replicati ⁇ n-competent" alphavirus genomic nucleic acid or alphavirus particle refers to the ability to replicate the viral genomic nucleic acid.
- a "replication-competent" alphavirus genomic nucleic acid or alphavirus particle will comprise sufficient alphavirus non-structural protein coding sequences (i.e., nsP1 through nsP4 coding sequences) to produce functional alphavirus non-structural proteins.
- the terms “deleted” or “deletion” mean either total deletion of the specified segment or the deletion of a sufficient portion of the specified segment to render the segment inoperative or nonfunctional, in accordance with standard usage.
- viruses e.g., replicating viruses
- inventive viral adjuvants enhance the immune response of a host (e.g., cellular and/or humoral response) against an immunogen that is independent of (e.g., is not presented by or encoded by) the viral adjuvant.
- the viral adjuvant of the invention enhances mucosal immunity against the immunogen.
- the viral adjuvants of the invention can be derived from any suitable virus.
- the viral adjuvant is an RNA viral adjuvant, i.e., comprises a viral genomic RNA (typically a modified form of a viral genomic RNA) or a DNA molecule that encodes a viral genomic RNA.
- the viral adjuvant can be a viral particle adjuvant, which comprises a live, live attenuated, killed and/or chimeric virus particle.
- the viral adjuvant comprises a replicating (i.e., replication-competent) virus particle.
- the viral particle adjuvant is an arbovirus (e.g., a flavivirus, alphavirus or virus in the family Bunyaviridae), a retrovirus, a rotavirus, an orthomyxovirus, a reovirus, a herpesvirus, a nidovirus, a ' norovirus, and/or a picomavirus.
- the viral adjuvant comprises a virus particle (including replicating virus particles) that uses a mucosal surface for viral entry into the host.
- the viral adjuvant comprises components derived from any of the foregoing viruses (e.g., structural proteins and/or nucleic acids, including replicating nucleic acids), optionally in a modified form.
- the viral adjuvant is an alphavirus adjuvant, more particularly a VEE viral adjuvant.
- alphavirus adjuvant or “VEE viral adjuvant” it is meant that the viral adjuvant comprises (1 ) a viral coat comprising one, two or more alphavirus or VEE structural proteins, respectively (e.g., E1 , E2 and/or capsid), for example, all of the viral structural proteins in the viral coat can be alphavirus or VEE structural proteins (e.g., E1 , E2 and capsid), respectively; and/or (2) an alphavirus or VEE genomic RNA (e.g., a replicating alphavirus or VEE genomic RNA), respectively; and/or (3) a DNA that encodes an alphavirus or VEE genomic RNA, respectively.
- a viral coat comprising one, two or more alphavirus or VEE structural proteins, respectively (e.g., E1 , E2 and/or capsid), for example, all of the viral structural proteins in the viral coat can be alphavirus or VEE structural proteins (e.g., E1 , E2 and capsid), respectively; and
- the alphavirus or VEE genomic RNA encompasses modified genomes.
- the alphavirus adjuvant comprises a replicating alphavirus or VEE virus particle, a replicating viral particle comprising an alphavirus or VEE virion coat, or a replicating viral particle comprising an alphavirus or VEE genomic RNA.
- an alphavirus adjuvant or VEE adjuvant comprising an alphavirus or VEE virion coat can further comprise a viral nucleic acid from another virus, either alphavirus or non- alphavirus.
- an alphavirus adjuvant or VEE adjuvant comprising an alphavirus or VEE genomic RNA can further comprise a virion coat from another virus, either alphavirus or non-alphavirus.
- the viral adjuvant can comprise a wild-type virus, an attenuated live virus and/or an inactivated (i.e., killed) virus.
- the viral adjuvant can comprise a viral genomic nucleic acid or can be a nucleic acid that encodes a nucleic acid derived from viral genomic nucleic acid, for example, as a liposomal formulation.
- the viral nucleic acid is replication-competent.
- the viral adjuvant comprises structural proteins assembled into a virus-like particle that does not package a genomic nucleic acid or the unassembled viral structural protein(s) (e.g., delivered as a liposomal formulation).
- the alphavirus E1 , E2 glycoproteins and/or the capsid protein, unassembled or assembled as an virus-like particle can be administered, for example, as a liposomal formulation.
- the viral adjuvant can further comprise one or more of the structural proteins (e.g., the alphavirus or VEE E1 and/or E2 glycoproteins) from one of the viruses described above so that the viral adjuvant targets to the same cell(s) as the virus from which the structural protein(s) is derived.
- the viral adjuvant comprises a viral nucleic acid (for example, a replicating viral nucleic acid) that is derived from one of the viruses described above.
- the viral adjuvant is a chimeric virus in which the structural proteins and genomic nucleic acid are derived from different viruses (e.g., two different alphaviruses such as Sindbis and VEE).
- the viral adjuvant is replication- competent (e.g., a replication-competent virus particle or viral nucleic acid).
- the viral adjuvant is a propagation-defective virus particle that cannot produce new virus particles upon infection of host cells.
- the viral adjuvant can be replication- competent in that it can infect a host cell and replicate and transcribe the viral genome, but cannot produce new virions (e.g., the virus is a replicon particle).
- the adjuvant virus comprises nonstructural protein sequences sufficient to provide replicase and transcriptase functions.
- the viral adjuvant is both propagation and replication-incompetent (e.g., an ultraviolet light or chemically inactivated virus).
- the viral adjuvant can be propagation-defective because it is defective for expression of (i.e., is unable to produce a functional form of) at least one or all of the viral structural proteins required to assemble new virus particles (e.g., alphavirus E1 , E2 and/or capsid proteins).
- the viral adjuvant comprises a modified viral genome or a nucleic acid that encodes a modified viral genome that is defective for expression of at least one viral structural protein required for production of new virus particles.
- the viral structural protein genes can be inactivated by a mutation and/or by deletion, tin representative embodiments, the viral adjuvant cannot produce any of the viral structural proteins.
- the modified viral genome lacks all or essentially all of the sequences encoding the viral structural proteins.
- the genomic promoter that drives expression of the viral structural protein genes is inactivated or deleted.
- the promoter can be inactivated by mutation of c/s-acting sequences, for example, by mutagenesis of sequence elements within the promoter region that are required for binding to the RNA polymerase complex.
- the viral polymerase can be mutated, e.g., mutation of the viral polymerase encoded by the alphavirus nsP4 gene.
- specific mutations in the alphavirus nsP1-nsP3 proteins are associated with loss of subgenomic RNA synthesis (while retaining genomic RNA synthesis).
- Such mutations can be incorporated into the viral adjuvants of the invention to render the viral adjuvant defective for subgenomic RNA synthesis as well as production of new virus particles.
- the viral adjuvant does not necessarily present or express the immunogen, i.e., the immunogen is not presented as part of the virion structure and the genome of the adjuvant virus does not comprise a heterologous nucleic acid sequence that encodes the immunogen.
- the adjuvant virus expresses a polypeptide of interest including but not limited to another immunogen, a reporter protein (e.g., an enzyme) and/or an immunomodulatory polypeptide such as a cytokine or chemokine (e.g., ⁇ -interferon, ⁇ -interferon, ⁇ -interferon, ⁇ - interferon, ⁇ -interferon, interleukin-1 ⁇ , interleukin-1 ⁇ , interleukin-2, interleukin- 3, interleukin-4, interleukin 5, interleukin-6, interleukin-7, interleukin-8, interleukin-9, interleukin-10, interleukin-11 , interleukin 12, interleukin-13, interleukin-14, interleukin-18, B cell Growth factor, CD40 Ligand, tumor necrosis factor- ⁇ , tumor necrosis fact ⁇ r- ⁇ , monocyte chemoattractant pfotein- 1 , granulocyte-m
- the viral adjuvant comprises a
- stuffer nucleic acid typically a "spacer” inserted in place of deleted structural protein coding sequences.
- the stuffer nucleic acid does not encode a polypeptide of interest or functional untranslated RNA, and is inserted into the genome to maintain the size of the genome in the range preferred by the virus (e.g., because of deletion of one or more of the viral structural protein genes).
- the viral adjuvant comprises any other nucleic acid that is transcribed and optionally translated, but does not encode the immunogen.
- the viral adjuvant does not comprise a heterologous nucleic acid that encodes a polypeptide of interest or functional untranslated RNA (i.e., the virus does not express a heterologous or foreign polypeptide of interest or functional untranslated RNA).
- the viral adjuvant does not comprise a foreign sequence that encodes a polypeptide of interest or functional untranslated RNA.
- the viral adjuvant is an "empty" virus particle or genomic nucleic acid that does not comprise a heterologous nucleic acid sequence (e.g., in place of deleted structural protein coding sequences).
- heterologous nucleic acid it is intended a nucleic acid that is foreign or exogenous to the virus and which is transcribed, and optionally translated, to produce a polypeptide of interest or functional untranslated RNA of interest or a "stuffer" nucleic acid as described above.
- the phrase "does not comprise a heterologous nucleic acid sequence” does not exclude the presence of all other foreign sequences in the virus, for example, foreign promoter sequences, attenuating mutations, mutations or foreign sequences that affect virus tropism, immunogenicity or virus clearance and/or other modifications that are introduced to alter pathogenesis, replication, transcription and/or translation.
- Such sequences do not encode a polypeptide of interest or functional untranslated RNA, as those terms are used herein.
- the viral adjuvant comprises a viral genomic nucleic acid that lacks sequences encoding the viral structural proteins and further in which the viral promoter that is operably associated therewith is inactivated or deleted therefrom.
- the adjuvant virus does not comprise a heterologous nucleic acid sequence (as described above).
- the viral adjuvant can be a "minimal" replication-competent nucleic acid or viral particle that lacks sequences encoding the structural proteins (i.e., is propagation incompetent) and the genomic promoter associated therewith, but does not comprises a heterologous nucleic acid in the form of a sequence that encodes a polypeptide of interest or functional untranslated RNA or a stuffer RNA.
- the "minimal" nucleic acid or virus particle comprises sequences necessary for the nucleic acid or virus particle to self-replicate.
- the viral adjuvant comprises: (a) a viral coat comprising virus structural proteins; and (b) a modified viral genome that lacks sequences encoding the viral structural proteins required for production of new virus particles; wherein the modified viral genome does not comprise a heterologous nucleic acid sequence that encodes a polypeptide of interest or a functional untranslated RNA.
- the viral adjuvant comprises one, two or more alphavirus structural proteins (e.g., all of the structural proteins in the virion coat are alphavirus structural proteins).
- the modified viral genome is a modified alphavirus. genome.
- the 26S promoter is inactivated or deleted.
- the viral adjuvant comprises a nucleic acid (e.g., DNA) that encodes the modified alphavirus genome.
- the viral adjuvant is a self-replicating viral adjuvant.
- the viral adjuvant can be a VEE viral adjuvant comprising a virion coat comprising one, two or more VEE structural proteins (e.g., all of the structural proteins in the virion coat are VEE structural proteins).
- the viral adjuvant is a VEE viral adjuvant comprising a modified VEE genome that lacks the sequences encoding the VEE structural proteins required for production of new virus particles.
- the VEE viral adjuvant comprises a modified viral genome that lacks sequences encoding the viral structural proteins.
- the modified viral genome does not comprise a heterologous nucleic acid sequence that encodes a polypeptide of interest or functional untranslated RNA.
- the VEE viral adjuvant comprises a nucleic acid (e.g., DNA) that encodes the modified VEE genome.
- the VEE 26S promoter is inactivated or deleted from the modified VEE genome.
- the VEE viral adjuvant is a self-replicating VEE viral adjuvant.
- the viral adjuvant is a VEE viral adjuvant comprising: VEE structural proteins; and a modified VEE genome that lacks the genes encoding the VEE structural proteins required for production of new virus particles.
- Alphavirus Adjuvants may be practiced using alphavirus adjuvants, for example, a propagation-incompetent, replicating, alphavirus vector such as an alphavirus replicon vector (as described below), an alphavirus-like particle of assembled structural proteins, or an alphavirus nucleic acid.
- alphavirus vectors, including replicon vectors are described in U.S. Patent No. 5,505,947 to Johnston et al.; U.S. Patent No. 5,792,462 to Johnston et al.; U.S. Patent No. 6,156,558; U.S. Patent No. 6,521 ,325; U.S. Patent No. 6,531 ,135; U.S. Patent No.
- the alphavirus vector is a Sindbis (e.g., TR339) or VEE vector, a Sindbis or VEE replicon vector, a Sindbis chimeric vector comprising a Sindbis genomic RNA or Sindbis glycoproteins (i.e., E1 and E2), or a VEE chimeric vector comprising a VEE genomic RNA or VEE glycoproteins.
- Sindbis e.g., TR339
- VEE vector e.g., TR339
- Sindbis or VEE replicon vector e.g., a Sindbis or VEE replicon vector
- Sindbis chimeric vector comprising a Sindbis genomic RNA or Sindbis glycoproteins (i.e., E1 and E2)
- E1 and E2 Sindbis glycoproteins
- VEE chimeric vector comprising a VEE genomic RNA or VEE glycoproteins.
- the alphavirus adjuvants employed in the present invention may be a chimeric alphavirus particle, as that term is understood in the art and defined herein.
- the alphavirus structural proteins may be from one alphavirus (e.g., VEE or a Sindbis virus such as TR339) and a genomic RNA packaged within the virion may be from another alphavirus.
- the alphavirus coat can be assembled from structural proteins derived from more than one alphavirus.
- the viral adjuvant comprises an alphavirus double promoter vector (e.g., a viral particle or a naked genomic RNA or a nucleic acid encoding the genomic RNA).
- a double promoter vector is typically a replication and propagation competent virus that retains the sequences encoding the alphavirus structural proteins sufficient to produce an alphavirus particle.
- Double promoter vectors are described in United States Patent No. 5,185,440, 5,505,947 and 5,639,650, the disclosures of which are incorporated in their entireties by reference.
- Illustrative alphaviruses for constructing the double promoter vectors are Sindbis (e.g., TR339), Girdwood and VEE viruses.
- the double promoter vector may contain one or more attenuating mutations. Attenuating mutations are described in more detail hereinbelow.
- the double promoter vector is constructed so as to contain a second subgenomic promoter (i.e., 26S promoter) inserted 3' to the viral RNA encoding the structural proteins or between nsP4 and the native 26S promoter.
- the heterologous RNA may be inserted between the second subgenomic promoter ⁇ so as to be operatively associated therewith, and the 3' UTR of the virus genome.
- Heterologous RNA sequences of less than 3 kilobases, more preferably thoseiess than 2 kilobases, and more preferably still those less than 1 kilobase, can be inserted into the double promoter vector.
- the double promoter vector is derived from a Sindbis (e.g., TR339) genomic RNA, and the second subgenomic promoter is a duplicate of the Sindbis (e.g., TR339) subgenomic promoter.
- the double promoter vector is derived from a VEE genomic RNA (e.g., having a mutation at nt3 of the genomic RNA), and the second subgenomic promoter is a duplicate of the VEE subgenomic promoter.
- the viral adjuvant can comprise an alphavirus replicon vector (e.g., a viral particle or naked genomic RNA or a nucleic acid encoding a genomic RNA), which are infectious, propagation-defective, replicating virus vectors.
- alphavirus replicon vectors are described in more detail in WO 96/37616 to Johnston et al.; U.S. Patent No. 5,505,947 to Johnston et al.; U.S. Patent No. 5,792,462 to Johnston et al.; U.S. Patent No. 6,156,558; U.S. Patent No. 6,521 ,325; U.S. Patent No. 6,531 ,135; U.S. Patent No.
- Illustrative alphaviruses for constructing the replicon vectors according to the present invention are Sindbis (e.g., TR339), Girdwood, VEE, and chimeras thereof.
- the viral genome contains the viral sequences necessary for viral replication (e.g., the nsp1-4 genes), but is modified so that it is defective for expression of at least one viral structural protein required for production of new viral particles.
- RNA transcribed from this vector contains sufficient viral sequences (e.g., the viral nonstructural genes) responsible for RNA replication and transcription.
- the viral nonstructural genes responsible for RNA replication and transcription.
- the autonomously replicating RNA (Ae., replicon) can only be packaged into virus particles if the defective or alphavirus structural protein genes that are deleted from or defective in the replicon are provided on one or more helper molecules, which are provided to the helper cell, or by a stably transformed packaging cell:*?'
- the helper molecules do not contain the viral nonstructural genes for replication, but these functions; are provided irrtrans by the replicon molecule.
- the transcriptase functions translated from the replicon molecule transcribe the structural protein genes on the helper molecule, resulting in the synthesis of viral structural proteins and packaging of the replicon into virus-like particles.
- the helper molecules do not contain a functional alphavirus packaging signal. As the alphavirus packaging or encapsidation signal is located within the nonstructural genes, the absence of these sequences in the helper molecules precludes their incorporation into virus particles.
- the replicon molecule is "propagation defective” or “propagation incompetent,” as described hereinabove.
- the resulting alphavirus particles are propagation defective inasmuch as the replicon RNA in these particles does not encode all of the alphavirus structural proteins required for encapsidation, at least a portion of at least one of the required structural proteins being deleted therefrom, such that the replicon RNA initiates only an abortive infection; no new viral particles are produced, and there is no spread of the infection to other cells.
- the replicon RNA may comprise one or more mutations within the structural protein coding sequences or promoter driving expression of the structural protein coding sequences, which interfere(s) with the production of a functional structural protein(s).
- the replicon molecule comprises an alphavirus packaging signal.
- the replicon molecule is self-replicating. Accordingly, the replicon molecule comprises sufficient coding sequences for the alphavirus nonstructural polyprotein so as to support self-replication.
- the replicon encodes the alphavirus nsP1 , nsP2, nsP3 and nsP4 proteins.
- the replicon molecules of the invention do not encode one or more of the capsid, E1 or E2 alphavirus structural proteins.
- do(es) not encode one or more structural proteins, it is intended that the replicon molecule does not encode a functional form of the one or more structural proteins and, thus, a complementing sequence must be provided by a helper or packaging cell to produce new virus particles.
- the replicon molecule does not encode any of the alphavirus structural proteins.
- the replicon may not encode the. structural protein(s) because the coding sequence is partially or entirely deleted from the replicon molecule. Alternatively, the coding sequence is otherwise mutated so that the replicon does not express the functional protein. In embodiments of the invention, the replicon lacks all or substantially all of the coding sequence of the structural protein(s) that is not encoded by the replicon, e.g., so as to minimize recombination events with the helper sequences.
- the replicon molecule may encode at least one, but not all, of the alphavirus structural proteins.
- the alphavirus capsid protein may be encoded by the replicon molecule.
- one or both of the alphavirus glycoproteins may be encoded by the replicon molecule.
- the replicon may encode the capsid protein and either the E1 or E2 glycoprotein.
- none of the alphavirus structural proteins are encoded by the replicon molecule.
- all or substantially all of the sequences encoding the structural proteins (e.g., E1 , E2 and capsid) may be deleted from the replicon molecule.
- a composition comprising a population of replicon particles of the invention contains no detectable propagation-competent alphavirus particles.
- Propagation-competent virus may be detected by any method known in the art, e.g., by neurovirulence following intracerebral injection into suckling mice, or by passage twice on alphavirus-permissive cells (e.g., BHK cells) and evaluation for virus induced cytopathic effects.
- Replicon vectors that do not encode the alphavirus capsid protein may nonetheless comprise a capsid translational enhancer region operably associated with a heterologous sequence, or the sequences encoding the non-structural proteins and/or encoding the alphavirus structural proteins (e.g., E1 and/or E2 glycoproteins) so as to enhance expression thereof.
- a capsid translational enhancer region operably associated with a heterologous sequence, or the sequences encoding the non-structural proteins and/or encoding the alphavirus structural proteins (e.g., E1 and/or E2 glycoproteins) so as to enhance expression thereof.
- the replicon vector is an "empty" replicon vector that does not comprise a heterologous nucleic acid sequence (as described herein) or a “minimal” replicon vector in which the 26S subgenomic promoter is deleted or inactivated (also as described herein).
- the methods of the present invention may also be carried out with alphavirus genomic RNA, structural proteins, and particles including attenuating mutations.
- attenuating mutation and "attenuating amino acid,” as used herein, mean a nucleotide sequence containing a mutation, or an amino acid encoded by a nucleotide sequence containing a mutation, which mutation results in a decreased probability of causing disease in its host (i.e., reduction in virulence), in accordance with standard terminology in the art. See, e.g., B. Davis et al., MICROBIOLOGY 132 (3d ed. 1980).
- the phrase "attenuating mutation” excludes mutations or combinations of mutations that would be lethal to the virus.
- Attenuating mutations will be dependent upon the alphavirus used, and will be known to those skilled in the art.
- Exemplary attenuating mutations include, but are not limited to, those described in United States Patent No. 5,505,947 to Johnston et al., U.S. Patent No. 5,185,440 to Johnston et al., U.S. Patent No. 5,643,576 to Davis et al., U.S. Patent No. 5,792,462 to Johnston et al., and U.S. Patent No. 5,639,650 to Johnston et al., the disclosures of which are incorporated herein in their entirety by reference.
- suitable attenuating mutations may be selected from the group consisting of codons at E2 amino acid position 76 which specify an attenuating amino acid, preferably lysine, arginine, or histidine as E2 amino acid 76; codons at E2 amino acid position 120 which specify an attenuating amino acid, preferably lysine as E2 amino acid 120; codons at E2 amino acid position 209 which specify an attenuating amino acid, preferably lysine, arginine or histidine as E2 amino acid 209; codons at E1 amino acid 272 which specify an attenuating amino acid, preferably threonine or serine as E1 amino acid 272; codons at E1 amino acid 81 which specify an attenuating amino acid, preferably isoleucine or leucine as E1 amino acid 81; codons at E1 amino acid 253 which specify an attenuating amino acid, preferably serine or thre
- Another suitable attenuating mutation is an attenuating mutation at nucleotide 3 of the VEE genomic RNA, i.e., the third nucleotide following the 5' methylated cap (see, e.g., U.S. Patent No. 5,643,576 describing a G->C mutation at nt 3).
- the mutation may be a G -> A, U or C, but is preferably a G-> A mutation.
- exemplary attenuating mutations in the structural and non-structural proteins include, but are not limited to, codons at nsP1 amino acid position 538 which specify an attenuating amino acid, preferably isoleucine as nsP1 amino acid 538; codons at E2 amino acid position 304 which specify an attenuating amino acid, preferably threonine as E2 amino acid 304; codons at E2 amino acid position 314 which specify an attenuating amino acid, preferably lysine as E2 amino acid 314; codons at E2 amino acid 372 which specify an attenuating amino acid, preferably leucine, at E2 amino acid residue 372; codons at E2 amino acid position 376 which specify an attenuating amino acid, preferably alanine as E2 amino acid 376; in combination, codons at E2 amino acid residues 304, 314, 372 and 376 which
- the attenuating mutation may be an attenuating mutation at amino acid position 537 of the S.A.AR86 nsP3 protein, more preferably a substitution mutation at this position (see, e.g., Table 1 below), still more preferably a nonsense mutation that results in substitution of a termination codon.
- Translational termination (i.e., stop) codons are known in the art, and include the "opal” (UGA), "amber” (UAG) and “ochre” (UAA) termination codons.
- the attenuating mutation results in a Cys->opal substitution at S.A.AR85 nsP3 amino acid position 537.
- Attenuating mutations include an attenuating insertion mutation following amino acid 385 of the S.A.AR86 nsP3 protein.
- the insertion comprises an insertion of at least 2, 4, 6, 8, 10, 12, 14, 16 or 20 amino acids.
- the inserted amino acid sequence is rich in serine and threonine residues (e.g., comprises at least 2, 4, 6, or 8 such sites) that serve as a substrate for phosphorylation by serine/threonine kinases.
- the attenuating mutation comprises an insertion of the amino acid sequence Ile-Thr-Ser-Met-Asp-Ser-Trp-Ser-Ser-Gly-Pro- Ser-Ser-Leu-Glu-lle-Val-Asp (SEQ ID NO:1) following amino acid 385 of nsP3 (i.e., the first amino acid is designated as amino acid 386 in nsP3).
- the insertion mutation comprises insertion of a fragment of SEQ ID NO:1 that results in an attenuated phenotype.
- the fragment comprises at least 4, 6, 8, 10, 12,14 or 16 contiguous amino acids from SEQ ID NO:1. i.
- Attenuating insertion sequences comprising a fragment of the sequence set forth above, or which incorporate conservative amino acid substitutions into the sequence set forth above, may be routinely identified by those of ordinary skill in the art (as described above). While not wishing to be bound by any theory, it appears that the insertion sequence of SEQ ID NO:1 is highly phosphorylated at serine residues, which confers an attenuated phenotype. Thus, other attenuating insertion sequences which serve as substrates for serine (or threonine) phosphorylation may be identified by conventional techniques known to those skilled in the art.
- the attenuating mutation comprises a Tyr->Ser substitution at amino acid 385 of the S.A.AR86 nsP3 (i.e., just prior to the insertion sequence above). This sequence is conserved in the non- virulent Sindbis-group viruses, but is deleted from S.A.AR86.
- Attenuating mutations for S.A.AR86 include attenuating mutations at those positions that diverge between S.A.AR86 and non- neurovirulent Sindbis group viruses, including attenuating mutations at nsP2 amino acid position 256 (preferably Arg -> Ala), 648 (preferably Ne -> VaI) or 651 (preferably Lys -> GIu), attenuating mutations at nsP3 amino acid position 344 (preferably GIy -> GIu), 441 (preferably Asp -> GIy) or 445 (preferably Ne - > Met), attenuating mutations at E2 amino acid position 243 (preferably Ser -> Leu), attenuating mutations at 6K amino acid position 30 (preferably VaI -> lie), and attenuating mutations at E1 amino acid positions 112 (preferably VaI -> Ala) or 169 (preferably Leu -> Ser).
- alphavirus adjuvants comprising an alphavirus capsid protein (or a nucleic acid encoding an alphavirus capsid protein) in which there is an attenuating mutation in the capsid protease that reduces, preferably ablates, the autoprotease activity of the capsid and results, therefore, in nonviable virus.
- capsid mutations that reduce or ablate the autoprotease activity of the alphavirus capsid are known in the art, see e.g., WO 96/37616 to Johnston et al., the disclosure of which is incorporated herein in its entirety.
- the alphavirus adjuvant comprises a VEE capsid protein in which the capsid protease is reduced or ablated, e.g., by introducing an amino acid substitution at VEE capsid position 152, 174, or 226.
- the homologous positions in other alphaviruses may be altered to reduce capsid protease activity.
- the attenuating mutation may be a mutation at capsid amino acid position 215 (e.g., a Ser->Ala) that reduces capsid autoprotease activity (see, Hahn et al., (1990) J. Virology 64:3069).
- Sindbis-group virus e.g., Sindbis, TR339, S.A.AR86, GirdwoodSA, Ockelbo
- capsid amino acid position 215 e.g., a Ser->Ala
- the attenuating mutations eliminate all pathology or adverse effects associated with administration of the viral adjuvant, as long as there is some improvement or benefit (e.g., increased safety and/or reduced morbidity and/or reduced mortality) as a result of the attenuating mutation.
- the attenuating mutation is an attenuating mutation in one or more of the cleavage domains between the alphavirus nonstructural (nsp) genes, e.g., the nsP1/nsP2 cleavage region, the nsP2/nsP3 cleavage region, and/or the nsP3/nsP4 cleavage region as described in PCT Application No.
- An exemplary attenuating mutation is a mutation at S.A.AR86 nsP1 amino acid 538 (position P3), more preferably a substitution mutation at S.A.AR86 nsP1 amino acid 538, still more preferably a Thr->lle substitution at S.A.AR86 nsP1 amino acid 538.
- the attenuating mutation reduces (e.g., by at least 25%, 40%, 50%, 60%, 70%, 80%, 90%, 95% or more) the neurovirulence of the alphavirus adjuvant (e.g., as determined by intracerebral injection in weanling or adult mice).
- the neurovirulence of the alphavirus adjuvant e.g., as determined by intracerebral injection in weanling or adult mice.
- amino acid substitutions may be based on any characteristic known in the art, including the relative similarity or differences of the amino acid side-chain substituents, for example, their hydrophobicity, hydrophilicity, charge, size, and the like.
- Amino acid substitutions other than those disclosed herein may be achieved by changing the codons of the genomic RNA sequence (or a DNA sequence), according to the following codon table:
- the hydropathic index of amino acids may be considered.
- the importance of the hydropathic amino acid index in conferring interactive biologic function on a protein is generally understood in the art (see, Kyte and Doolittle, (1982) J. MoI. Biol. 157:105; incorporated herein by reference in its entirety). It is accepted that the relative hydropathic character of the amino acid contributes to the secondary structure of the resultant protein, which in turn defines the interaction of the protein with other molecules, for example, enzymes, substrates, receptors, DNA, antibodies, antigens, and the like.
- the hydropathic index of the amino acid may be considered when identifying additional attenuating mutations according to the present invention.
- hydrophilicity values have been assigned to amino acid residues: arginine (+3.0); lysine ( ⁇ 3.0); aspartate (+3.0 ⁇ 1 ); glutamate (+3.0 ⁇ 1 ); threonine (+0.3); asparagine (+0.2); glutamine (+0.2); glycine (0); threonine, ⁇ -0.4); proline (-0.5 ⁇ I); alanine (-0.5); histidine (-0.5); cysteine (-1.0); methionine (-1.3); valine (-1.5); leucine (-1.8); isoleucine (-1.8); tyrosine (-2.3); ⁇ henylalanine (-2.5); tryptophan (-3.4).
- hydrophilicity of the amino acid may be considered when identifying additional attenuating mutations according to the present invention.
- Mutations may be introduced into the alphavirus genome by any method known in the art.
- mutations may be introduced into the alphavirus RNA by performing site-directed mutagenesis on the cDNA which encodes the RNA, in accordance with known procedures (see, Kunkel, Proc. Natl. Acad. Sci. USA 82, 488 (1985), the disclosure of which is incorporated herein by reference in its entirety).
- mutations may be introduced into the RNA by replacement of homologous restriction fragments in the cDNA which encodes for the RNA in accordance with known procedures.
- FIG. 1 Further aspects of the present invention are methods and helper cells for producing the alphavirus adjuvants.
- Methods and helper cells for producing alphavirus particles including double-promoter alphaviruses and alphavirus replicon particles are known in the art. See, e.g., Patent No. 5,185,440 to Davis et al., U.S. Patent No. 5,505,947 to Johnston et al.; U.S. Patent No. 5,792,462 to Johnston et al.; U.S. Patent No. 6,156,558; U.S. Patent No. 6,521 ,325; U.S. Patent No. 6,531 ,135; U.S. Patent No. 6,541 ,010; and Pushko et al. (1997) Virol. 239:389-401 ; the disclosures of which are incorporated herein by reference in their entireties.
- the methods and helper cells are used to produce propagation-incompetent alphavirus particles, more preferably, propagation-incompetent alphavirus replicon particles.
- the helper cells contain one or more helper nucleic acid sequences (e.g., as DNA and/or RNA molecules) encoding the alphavirus structural proteins (e.g., VEE structural proteins).
- the combined expression of the replicon molecule and the one or more helper molecules in the helper cell results in the production of an assembled alphavirus 'particle comprising a replicon RNA packaged within a virion comprising alphavirus structural proteins, which is able to infect a cell, but is unable to produce a productive infection (i.e., produce new virus particles).
- the population of alphavirus particles contains no detectable propagation-competent alphavirus particles.
- Propagation- competent virus may be detected by any method known in the art, e.g., by neurovirulence following intracerebral injection into suckling mice, or by passage twice on alphavirus-permissive cells (e.g., BHK cells) and evaluation for virus induced cytopathic effects.
- the present invention provides a method of producing a recombinant alphavirus particle in vitro, comprising: providing to a cell in culture: (a) a modified alphavirus genomic RNA, comprising (i) sequences encoding the ' aiphavirus nonstructural proteins, and (ii) a competent alphavirus packaging sequence, and further (iii) wherein the genomic RNA is defective for the expression of at least one viral structural protein required for production of new virus particles; (b) sequences encoding the alphavirus structural proteins sufficient for producing an alphavirus particle; wherein the combined expression of the alphavirus replicon RNA and the sequences encoding the alphavirus structural proteins produces an assembled alphavirus particle comprising the modified alphavirus genomic RNA; and producing an alphavirus particle in the cell.
- the replicon RNA can comprise a heterologous RNA that encodes a polypeptide of interest or functional untranslated RNA.
- the replicon does not comprise a heterologous RNA that encodes a polypeptide of interest or functional untranslated RNA.
- the replicon can optionally be an "empty" replicon that does not comprise a heterologous nucleic acid (e.g., in place of the deleted structural protein genes) or, further, a "minimal" replicon in which the 26S promoter is deleted or inactivated.
- the helper cell furtherlcomprises one or more replicon molecules, as described herein.
- helper sequences will not include an alphavirus packaging sequence, whereas the replicon will contain an alphavirus packaging sequence (e.g., a VEE packaging sequence).
- alphavirus packaging sequence e.g., a VEE packaging sequence
- the helper cells are typically alphavirus-permissive cells.
- Alphavirus- permissive cells employed in the methods of the present invention are cells that, upon transfection with an alphavirus genomic RNA (or a nucleic acid encoding the same), are capable of producing viral particles.
- Preferred alphavirus- permissive cells are Sindbis permissive cells (e.g., TR339-permissive cells), VEE permissive cells, S.A.AR86-permissive cells, Girdwood-permissive cells, and Semliki Forest virus-permissive cells.
- Alphaviruses have a broad host range. Examples of suitable host cells include, but are not limited to fibroblasts, Vera cells, baby hamster kidney (BHK) cells, 293 cells, 293T cells, and chicken embryo fibroblast cells (e.g., DF- 1 cells).
- helper cells of the invention may further comprise sequences encoding the alphavirus structural proteins sufficient to produce an alphavirus particle, as described herein.
- the helper cell may comprise a replicon RNA, also as described herein.
- sequences encoding the alphavirus structural proteins are distributed among one or more helper molecules (optionally, two or three helper RNAs).
- one or more structural proteins may be encoded by the replicon RNA, provided that the replicon RNA does not encode at least one structural protein such that the resulting alphavirus particle is propagation-incompetent in the absence of the helper sequence(s).
- At least one of the alphavirus structural and/or non-structural proteins encoded by the replicon and helper molecules can contain one or more attenuating mutations, as described herein, a
- the helper sequences and/or replicon may further comprise an alphavirus capsid enhancer sequence (e.gv, a S.A.AR86 capsid enhancer sequence), as described above.
- an alphavirus capsid enhancer sequence e.gv, a S.A.AR86 capsid enhancer sequence
- a S.A.AR86 capsid enhancer sequence as described above. See, e.g., PCT Application No. PCT/U S01/27644; U.S. Patent No.i.6,224,879 to Sjoberg et al., Smerdou et al., (1999) J. Virology 73: 1092; Frolov et al., (1996) J. Virology 70:1182; and Heise et al. (2000) J. Virol. 74:9294-9299 (the disclosures of which are incorporated herein in their entireties).
- the replicon molecule encodes at least one, but not all, of the alphavirus structural proteins (e.g., the E1 and/or E2 glycoproteins and/or the capsid protein).
- the replicon can encode the capsid protein, and the E1 and E2 glycoproteins are encoded by one or more separate helper molecules.
- the glycoproteins are encoded by two separate helper molecules, so as to minimize the possibility of recombination to produce replication-competent virus.
- the replicon does not encode any of the E1 glycoprotein, the E2 glycoprotein, or the capsid protein.
- the capsid protein and alphavirus glycoproteins are encoded by one or more helper molecules, optionally two or more helper molecules.
- the replicon does not encode any of the alphavirus structural proteins, and may lack the sequences encoding the alphavirus structural proteins.
- the replicon may not encode the structural protein(s) because of a partial or complete deletion of the coding sequence(s) or otherwise contains a mutation that prevents the expression of a functional protein(s).
- all or substantially all of the coding sequences for the structural protein(s) that is not encoded by the replicon are deleted from the replicon molecule.
- the E1 and E2 glycoproteins are encoded by one helper molecule, and the capsid protein is encoded by another helper ⁇ - molecule.
- the E1 glycoprotein, E2 glycoprotein, and capsid protein are each encoded by separate helper molecules.
- the capsid protein and one of the glycoproteins are encoded by one ⁇ elper molecule; and the other glycoprotein is encoded by a second helper molecule.
- duplicate copies of the structural proteins are not present among the replicon and helper molecules. If duplicate copies of the structural proteins are present, generally other approaches are used to avoid recombination events and/or generation of replication-competent virus.
- helper and replicon sequences can be RNA molecules that are introduced into the cell, e.g., by lipofection or electroporation.
- Uptake of helper RNA and replicon RNA molecules into packaging cells in vitro can be carried out according to any suitable means known to those skilled in the art. Uptake of RNA into the cells can be achieved, for example, by treating the cells with DEAE-dextran, treating the RNA with LIPOFECTINTM before addition to the cells, or by electroporation, with electroporation being the currently preferred means. These techniques are well known in the art. See e.g., United States Patent No. 5,185,440 to Davis et al., and PCT Publication No.
- helper and/or replicon molecules are DNA molecules, which are optionally stably integrated into the genome of the helper cell or expressed from an episome (e.g., an EBV derived episome).
- the DNA molecule may be any vector known in the art, including but not limited to a non-integrating DNA vector, such as a plasmid, or a viral vector.
- the invention provides nucleic acids encoding the viral adjuvants of the invention.
- the present invention provides DNA sequences (e.g., cDNA sequences) and vectors encoding infectious modified alphavirus genomic RNA transcripts (e.g., VEE genomic transcripts) as described herein.
- alphavirus particles containing the genomic RNA transcribed from the DNA molecules are also provided.
- the present invention further provides vectors and constructs comprising a DNA sequence encoding the alphavirus genomic 'RNA transcript described above operably associated with a promoterithat drives transcription of the DNA sequence.
- promoters which are suitable for use with the DNA sequences of the present invention include, but are not limited to T3 promoters, T7 promoters, cytomegalovirus (CMV) promoters, and SP6 promoters.
- the DNA sequence may be embedded within any suitable vector known in the art, including but not limited to, plasmids, naked DNA vectors, yeast artificial chromosomes (yacs), bacterial artificial chromosomes (bacs), phage, viral vectors, and the like.
- the vector is a plasmid.
- Genomic RNA transcripts may be synthesized from the DNA template by any method known in the art.
- the RNA is synthesized from the DNA sequence in vitro using purified RNA polymerase in the presence of ribonucleotide triphosphates and cap analogs in accordance with conventional techniques.
- the RNA may be synthesized intracellular ⁇ after introduction of the DNA.
- cells containing the DNA sequences, genomic RNA transcripts, and alphavirus vectors of the invention include, but are not limited to, fibroblast cells, Vera cells, Baby Hamster Kidney (BHK) cells, Chinese Hamster Ovary (CHO) cells, macrophages, dendritic cells, and the like.
- C. Compositions Comprising the Viral Adjuvant and an Immunoqen.
- compositions including pharmaceutical compositions, comprising the viral adjuvant and an immunogen.
- the immunogen can be any immunogen known in the art and can be administered in any suitable form.
- the immunogen can be in the form of a live, attenuated live, or killed (i.e., inactivated) organism (e.g., a bacterium or protozoan) or virus, or an extract or toxoid thereof.
- the immunogen can be provided as an isolated component (e.g., a polypeptide or a peptide [e.g., from about 6 to 20 or 8 to 12 amino acids in length]).
- the jmmunogencan be administered perse or can be expressed from a nucleic acid that is administered to the host and the immunogen expressed therefrom.
- a delivery vector such as a recombinant, viral vector or a plasmid that expresses the immunogen
- the immunogen can comprise B cell and/or T cell epitopes as are known in the art.
- the immunogen can further be soluble or particulate (e.g., microspheres).
- the immunogen can be present in the organism.
- the subject fails to mount a sufficient immune response against the antigen.
- the viral adjuvants of the invention can be administered to the subject to induce an immune response against the antigen already present in the subject as a result of the infection.
- the immunogen can be an immunogen from an infectious agent, a cancer immunogen, an allergic reaction immunogen (i.e., an allergen), a transplantation immunogen, an autoantigen, and the like as are known in the art.
- a cancer immunogen i.e., an immunogen associated with cancer cells, optionally specifically associated with cancer cells
- MAGE-1 , MAGE-2, MAGE-3, MAGE-4 and MAGE-11 Members of the GAGE family include, but are not limited to, GAGE-1 , GAGE-6. See, e.g., review by Van den Eynde and van der Bruggen (1997) in Curr. Opin. Immunol. 9: 684-693, Sahin et al. (1997) in Curr. Opin..Immunol. 9: «709-716, and Shawler et al. (1997), the entire contents of which are incorporated by reference herein for their teachings of cancer antigens. ' ' ⁇ . • ' " • * ⁇ >
- the cancer immunogen can also be ⁇ but is not limited to, human epithelial cell mucin (Muc-1 ; a 20 amino acid core repeat for Muc-1 glycoprotein, present on breast cancer cells and pancreatic cancer cells), MUC-2, MUC-3, MUC-18, the Ha-ras oncogene product, carcino-embryonic antigen (CEA), the raf oncogene product, CA-125, GD2, GD3, GM2, TF, sTn, gp75, EBV-LMP 1 & 2, HPV-F4, 6, 7, prostatic serum antigen (PSA), prostate- specific membrane antigen (PSMA), alpha-fetoprotein (AFP), CO17-1A, GA733, gp72, p53, the ras oncogene product, ⁇ -HCG, gp43, HSP-70 , p17 mel, HSP-70, gp43, HMW, HOJ-1 , melanoma ganglio
- the cancer immunogen can also be an antibody produced by a B cell tumor (e.g., B cell lymphoma; B cell leukemia; myeloma; hairy cell leukemia), a fragment of such an antibody, which contains an epitope of the idiotype of the antibody, a malignant B cell antigen receptor, a malignant B cell immunoglobulin idiotype, a variable region of an immunoglobulin, a hypervariable region or complementarity determining region (CDR) of a variable region of an immunoglobulin, a malignant T cell receptor (TCR), a variable region of a TCR and/or a hypervariable region of a TCR.
- a B cell tumor e.g., B cell lymphoma; B cell leukemia; myeloma; hairy cell leukemia
- a fragment of such an antibody which contains an epitope of the idiotype of the antibody
- a malignant B cell antigen receptor e.g., a malignant
- the cancer antigen of this invention can be a single chain antibody (scFv), comprising linked VH, and VL domains, which retains the conformation and specific binding activity of the native idiotype of the antibody.
- the immunogens that can be used in accordance with the present invention are in no way limited to the cancer immunogens listed herein.
- Other cancer immunogens can be identified, isolated and cloned by methods known in the art such as those disclosed in U.S. Pat. No. 4,514,506, the entire contents of which are incorporated by reference herein.
- the cancer to be treated or immunized against (Ae., prophylactic treatment) by administration to a subject of a viral adjuvant of this invention can be, but is not limited to, B cell lymphoma, T cell lymphoma, myeloma, leukemia, hematopoietic neoplasias, thymoma, lymphoma, sarcoma, lung cancer, liver cancer, non-Hodgkins lymphoma, Hodgkins lymphoma, uterine cancer, adenocarcinoma, breast cancer, pancreatic cancer, colon cancer, lung cancer, renal cancer, bladder cancer, liver cancer, prostate cancer, ovarian cancer, primary or metastatic melanoma, squamous ceil carcinoma, basal cell carcimona, brain cancer, angiosarcoma, hemangiosarcoma, head and neck carcinoma, thyroid carcinoma, soft tissue sarcoma, bone sarcoma, testicular cancer, uter
- Infectious agent immunogens can include any immunogen suitable for protecting a subject against an infectious disease, including but not limited to microbial, bacterial, protozoal, parasitic and viral diseases.
- infectious agent immunogens can include, but are not limited to, immunogens from Hepadnaviridae including hepatitis A, B, C, D, E, F, G, etc.; Flaviviridae including human hepatitis C virus (HCV), yellow fever virus and dengue viruses; Retroviridae including human immunodeficiency viruses (HIV), simian immunodeficiency virus (SIV), and human T lymphotropic viruses (HTLV1 and HTLV2); Herpesviridae including herpes simplex viruses (HSV-1 and HSV-2), Epstein Barr virus (EBV), cytomegalovirus, varicella-zoster virus (VZV), human herpes virus 6 (HHV-6) human herpes virus 8 (HHV-8), and herpes B virus; Papovaviridae including human papilloma viruses; Rhabdoviridae including rabies virus; Paramyxoviridae including respiratory syncytial virus; Reoviridae including
- the immunogen may be an orthomyxovirus immunogen (e.g., an influenza virus immunogen, such as the influenza virus hemagglutinin (HA) surface protein, influenza neuraminidase protein, the influenza virus nucleoprotein (NP) antigen or inactivated influenza virions, or an equine influenza virus immunogen), or a lentivirus immunogen (e.g., an equine infectious anemia virus immunogen, a SIV immunogen, or a HIV immunogen, such as, e.g., HIV or SIV gp120, gp160, gp41 , or matrix/capsid protein, or the gag, pol or env gene products).
- an influenza virus immunogen such as the influenza virus hemagglutinin (HA) surface protein, influenza neuraminidase protein, the influenza virus nucleoprotein (NP) antigen or inactivated influenza virions, or an equine influenza virus immunogen
- a lentivirus immunogen
- the immunogen may also be an arenavirus immunogen (e.g., Lassa fever virus immunogen, such as the Lassa fever virus nucleocapsid protein gene and the Lassa fever envelope glycoprotein gene), a Picornavirus immunogen (e.g., a Foot and Mouth Disease virus immunogen), a poxvirus immunogen (e.g., a vaccinia immunogen, such as the vaccinia L1 or L8 genes), an Orbivirus immunogen (e.g., an African horse sickness virus immunogen), a flavivirus immunogen (e.g., a yellow fever virus immunogen, a West Nile virus immunogen, or a Japanese encephalitis virus immunogen), a filovirus immunogen (e.g., an Ebola virus immunogen, or a Marburg virus immunogen, such as NP and GP genes), a bunyavirus immunogen (e.g., RVFV, CCHP ⁇ and SFS immunogens), a norovirus immunogen (e
- the immunogen may further be a polio antigen, herpes antigen (e.g., CMV, EBV, HSV antigens) mumps antigen, measles antigen, rubella antigen, diptheria toxin or other diptheria antigen, pertussis antigen, hepatitis (e.g., hepatitis A or hepatitis B) antigen (e.g., HBsAg, HBcAg, HBeAg), or any other vaccine immunogen known in the art.
- herpes antigen e.g., CMV, EBV, HSV antigens
- mumps antigen e.g., measles antigen, rubella antigen, diptheria toxin or other diptheria antigen, pertussis antigen
- hepatitis e.g., hepatitis A or hepatitis B
- antigen e.g., HBsAg, HBcAg,
- the immunogen can be an immunogen from a pathogenic microorganism, which can include but is not limited to, Rickettsia, Chlamydia, Mycobacteria, Clostridia, Corynebacteria, Mycoplasma, Ureaplasma, Legionella, Shigella, Salmonella, pathogenic Escherichia coli species, Bordatella, Neisseria, Treponema, Bacillus, Haemophilus, Moraxella, Vibrio, Staphylococcus spp., Streptococcus spp., Campylobacter spp., Borrelia spp., Leptospira spp., Erlichia spp., Klebsiella spp., Pseudomonas spp., Helicobacter spp., and any other pathogenic microorganism now known or later identified (see, e.g., Microbiology, Davis et al, Eds.,
- microorganisms from which the immunogen of this invention can be obtained include, but are not limited to, Helicobacter pylori, Chlamydia pneumoniae, Chlamydia trachomatis, Ureaplasma urealyticum, Mycoplasma pneumoniae, Staphylococcus aureus, Streptococcus pyogenes, Streptococcus pneumoniae, Streptococcus viridans, Enterococcus faecalis, Neisseria meningitidis, Neisseria gonorrhoeae, Treponema pallidum, Bacillus anthracis, Salmonella typhi, Vibrio cholera, Pasteurella pestis, Pseudomonas aeruginosa, Campylobacter jejuni, Clostridium difficile, Clostridium botulinum, Mycobacterium tuberculosis, Borrelia burgdorferi, Haemophilus du
- the immunogen can further be an immunogen from a pathogenic protozoa, including, but not limited to, Plasmodium species (e.g., malaria antigens), Babeosis species, Schistosoma species, Trypanosoma species, Pneumocystis carnii, Toxoplasma species, Leishmania species, and any other protozoan pathogen now known or later identified.
- the immunogen can also be an immunogen from pathogenic yeast and fungi, including, but not limited to, Aspergillus species, Candida species, Cryptococcus species, Histoplasma species, Coccidioides species, and any other pathogenic fungus now known or later identified.
- immunogens include, but are not limited to, the influenza virus nucleoprotein (residues 218-226; Fu et al. (1997) J. Virol. 71 : 2715-2721 ), antigens from Senclai virus and lymphocytic choriomeningitis virus (An et al. (1997) J. Virol. 71 : 2292-2302), the B1 protein of hepatitis C virus (Bruna-Romero et al. (1997) Hepatology 25: 470-477), gp 160 of HIV (Achour et al. (1996) J. Virol.
- E7 protein (residues 21-28 and 48-55; Bauer et al. (1995) Scand. J. Immunol. 42: 317-323) of human papillomavirus type 16, the M2 protein of respiratory syncytial virus (residues 82-90 and 81-95; Hsu et al. (1995) Immunology 85: 347-350), the herpes simplex virus type 1 ribonucleotide reductase (Salvucci et al. (1995) J. Gen. Virol. 69: 1122-1131 ), the rotavirus VP7 protein (Franco et al. (1993) J. Gen.
- the immunogen can also be an immunogen from chronic or latent infective agents, which typically persist because they fail to elicit a strong immune response in the subject.
- Illustrative latent or chronic infective agents include, but are not limited to, hepatitis B, hepatitis C, Epstein-Barr Virus, herpes viruses, human immunodeficiency virus, and human papilloma viruses.
- the adjuvant is beneficial because (1) it mayinduce proinflammatory cytokines and interferon, and/or (2) it acts as an adjuvant to enhance the immune response against the antigen that is already present from the infection.
- the viral adjuvants of the invention can be used to produce an immune response to, and optionally to treat or to prevent infection (i.e., prophylactic treatment) from any infectious agent, including but not limited to those identified above.
- Suitable transplantation immunogens include, but are not limited to, different antigenic specificities of HLA-A, B and C Class I proteins. Different antigenic specificities of HLA-DR, HLA-DQ, HLA-DP and HLA-DW Class Il proteins can also be used (WHO Nomenclature Committee, lmmunogenetics 16:135 (1992); Hensen et al., in Fundamental Immunology, Paul, Ed., pp. 577-628, Raven Press, New York, 1993; NIH Genbank and EMBL data bases).
- Immunogens that are allergens are also contemplated by the present invention, which can include but are not limited to, environmental allergens such as dust mite allergens; plant allergens such as pollen, including ragweed pollen; insect allergens such as bee and ant venom; and animal allergens such as cat dander, dog dander and animal saliva allergens.
- environmental allergens such as dust mite allergens
- plant allergens such as pollen, including ragweed pollen
- insect allergens such as bee and ant venom
- animal allergens such as cat dander, dog dander and animal saliva allergens.
- the immunogen is a ragweed allergen or a grass allergen.
- Ragweed and in particular Short Ragweed (Ambrosia artemisiifolia), is clinically the most important source of seasonal aeroallergens, as it is responsible for both the majority of cases and the most severe cases of allergic rhinitis (Pollart, et al. (1989) J. Allergy Clin. Immunol. 83(5):875-82; Rosenberg, et al. (1983) J. Allergy Clin. Immunol. 71 (3):302-10; Bruce, et al. (1977) J. Allergy Clin. Immunol. 59(6): 449-59).
- Ragweed pollen also contributes significantly to exacerbation of asthma and allergic conjunctivitis.
- Grass pollen is the most frequent cause of hay fever. Although more than 1 ,000 species of grass grow in North America, only a few produce highly allergenic pollen. These include Timothy grass, Kentucky bluegrass, Johnson grass, Bermuda grass, redtop grass, orchard grass, velvet grass, rye grass and sweet vernal grass. u
- the immunogen is an allergen from ragweed or a grass species including, but not limited to, those listed Table II.
- the weed and grass allergens provided in Table Il are members of the polysaccharide lyase family 1 of proteins (e.g., Amb a 1 and Amb a 2); lipid transfer proteins (e.g., Parj 1 , Parj 2, Par o 1); profilins (e.g., HeI a 2, Mer a 1 , Che a 2, Parj 3, Cyn d 12); polcalcins (e.g., Che a 3), or proteins with homology to trypsin inhibitors (e.g., PhI p 11 ).
- proteins e.g., Amb a 1 and Amb a 2
- lipid transfer proteins e.g., Parj 1 , Parj 2, Par o 1
- profilins e.g., HeI a 2, Mer a 1 , Che a 2, Parj 3, Cyn d 12
- polcalcins e.g., Che a 3
- proteins with homology to trypsin inhibitors
- the immunogen is an allergen isolated from trees such as oak (e.g., Que a 1 ), mountain cedar (e.g, Jun a 1-Jun a 3), birch (e.g., Bet v 1-Bet v 7), ash (e.g, Fra e 1), alder (e.g., AIn g 1 ), hazel (e.g., Cor a 1 , Cor a 2, or Cor a 8-Cor a 11 ), juniper (e.g., Jun o 4), and cypress (e.g., Cup a 1 ); molds such as Alternaria alternata (e.g., Alt a 1-AIt a 4, Alt a 6, Alt a 7, or Alt a 10- Alt a 12), Cladosporium herbarum (e.g., CIa h 1-CIa h 6, or CIa h 12), Aspergillus flavus (e.g., Asp fl 13), A.
- trees such
- fumigatus e.g., Asp f 1-Asp f 13, Asp f 15-Asp f 18, Asp f 22w or Asp f 23
- A. niger e.g., Asp n 14 or Asp n 18
- A. oryzae e.g., Asp o 13 or Asp o 21
- Penicillium brevicompactum e.g., Pen ch 13, Pen ch 18, or Pen ch 20
- P. citrinum e.g., Pen c 3, Pen c 13, Pen. c 19, Pen c 22w, or Pen c 24
- oxalicum e.g., Pen o 18
- Epicoccum purpurascens e.g., Epi p 1
- Fusarium culmorum e.g., Fus c 1 or Fus c 2
- egg whites e.g., GaI d 1-GaI d 5
- milk e.g., Bos d 4-Bos d 8
- wheat e.g., Tri a 18 and Tri a 19
- cat FeI d 1-FeI d 7w
- dust mites e.g., Aca s 13, Blo t 1 , Blo t 3- Blo t 6, BIo 1 10- BIo 1 13, Blo t 19, Der f 1-Der f 3, Derf 7, Der f 10, Der f 11 , Der f 14- Der f 18w, Der m 1 , Der p 1-Der p iO, Der p 14, Eur m 2, Eur m 14, GIy d
- the immunogen can further be an autoantigen (for example, to enhance self-tolerance to an autoantigen in a subject, e.g., a subject in whom self-tolerance is impaired).
- autoantigens include, but are not limited to, myelin basic protein, islet cell antigens, insulin, collagen and human collagen glycoprotein 39, muscle acetylcholine receptor and its separate polypeptide chains and peptide epitopes, glutamic acid decarboxylase and muscle-specific receptor tyrosine kinase.
- the viral adjuvants of the invention can be used for a variety of purposes.
- the present invention finds use in methods of producing antibodies in vivo for passive immunization techniques.
- a viral adjuvant and immunogen of interest are administered to a subject, either by direct administration or ex vivo cell manipulation techniques as known in the art.
- the antibody can then be collected from the subject using routine methods known in the art.
- the invention further finds use in methods of producing antibodies against an immunogen for any other purpose, e.g., for diagnostics or for use in histological techniques.
- the viral adjuvants can further be used in therapeutic and/or prophylactic methods, for veterinary and/or medical purposes.
- the adjuvant virus of the invention can be administered to the subject as a general immune enhancer to increase both innate and adaptive immune function in the subject, for example, immunocompromised subjects such as subjects undergoing chemotherapy, radiation therapy, subjects with chronic infections (e.g., HCV and HBV) and/or subjects with HIV/AIDs.
- the invention can further be practiced to enhance the immune response to an attenuated live virus, a killed vaccine, or a DNA vaccine, all of which can have the disadvantage of reduced immunogenicity.
- the adjuvant virus of the invention can be administered to treat a chronic or latent infection to induce or enhance the immune response against the antigen(s) produced by the infection.
- Suitable subjects according to the present invention can be any animal subject (e.g., avians and mammalian subjects).
- Mammalian subjects include but are not limited to humans, non-human primates, dogs, cats, pigs, goats, sheep, cattle, horses, mice, rats and rabbits.
- Avian subjects include but are not limited to chickens, turkeys, ducks, geese, quail, and birds kept as pets (e.g., parakeets, parrots, macaws, cockatoos, and the like).
- the invention provides a method of producing an immune response against an immunogen in a subject, the 5 method comprising: (a) administering the immunogen to the subject in an immunogenically effective amount; and (b) administering the viral adjuvant to the subject in an adjuvant effective amount, wherein the viral adjuvant does not express the immunogen.
- the viral adjuvant is administered as an adjuvant to enhance the immune
- Viral adjuvants are as described hereinabove.
- the invention also provides a method of producing an immune response against an immunogen in a subject, the method comprising: (a) administering the immunogen to the subject in an immunogenically effective
- an alphavirus e.g., a propagation-defective ⁇ ⁇ . alphavirus
- an alphavirus e.g., a propagation-defective ⁇ ⁇ . alphavirus
- an alphavirus adjuvant to the subject in an adjuvant effective amount, wherein >. i . the alphavirus adjuvant does not express the immunogen.
- the invention provides a method of producing an immune response against an immunogen in a subject, the
- VEE viral adjuvant e.g., a propagation-defective VEE viral adjuvant
- the invention provides for the use of a viral adjuvant for increasing the immune response against an immunogen. Also encompassed by the invention is the use of a virus for the preparation of an adjuvant formulation. Further provided is the use of a viral adjuvant for the preparation of a medicament.
- an immunogenically effective amount is an amount of the immunogen that is sufficient to evoke an active immune response (cellular and/or humoral) in a subject that is co-administered the adjuvant.
- an immunogenically effective amount is sufficient to produce a protective immune response. The degree of protection conferred need not be complete or permanent.
- an “adjuvant effective amount” is an amount of the adjuvant virus that is sufficient to enhance or stimulate the active immune response (cellular and/or humoral) mounted by the host against the immunogen, optionally an active mucosal immune response.
- the active immune response e.g., a mucosal immune response
- an “adjuvant effective amount” is an amount !
- an "adjuvant effective amount” can refer to an amount of the viral adjuvant that accelerates the induction of the immune response in the host and/or reduces the need for booster immunizations to achieve protection.
- an “adjuvant effective amount” can be an amount that prolongs the time period over which an immune response, optionally protective immune response, is sustained (e.g., by at least about a 2-fold, 3- fold, 5-fold, 10-fold, 20-fold longer time period or more).
- An “active immune response” or “active immunity” is characterized by
- an active immune response is mounted by the host after exposure to immunogens by infection or by vaccination. Active immunity can be contrasted with passive immunity, which is acquired through the "transfer of preformed substances (antibody, transfer factor, thymic graft, interleukin-2) from an actively immunized host to a non-immune host.” Id.
- a “protective” immune response or “protective” immunity as used herein indicates that the immune response confers some benefit to the subject in that it prevents or reduces the incidence and/or severity of disease.
- a protective immune response or protective immunity may be useful in the therapeutic treatment of disease.
- Suitable dosages of the immunogen and viral adjuvant will vary depending upon the condition, age and species of the subject, the nature of the immunogen, the nature of the viral adjuvant, the level of immunogenicity and enhancement desired, and like factors, and can be readily determined by those skilled in the art.
- the dosage of the viral adjuvant is greater than about AO '2 , 10 " Vi 0, 10 2 , 10 3 , 10 4 , 10 5 or 10 6 virus particles, virus-like particles, or infectious units and/or less than about 10 2 , 10 3 10 4 , 10 5 ,10 6 , 10 7 , 10 8 , 10 9 , 10 10 , 10 1 ViO 12 , 10 13 or even 10 14 or more virus particles, virus-like particles, or infectious units.
- Those skilled in the art will appreciate that some methods of titering viruses have relatively low sensitivity giving rise to apparent titers of less than one virus particle.
- Viral titers can be assessed by any method known in the art, including cytotoxicity in cultured cells ⁇ e.g., alphavirus titers can be assessed by cytotoxicity in BHK cells). In other representative embodiments, a dosage of about 10 "1 to 10 7 , 10 to 10 6 or about 10 2 to 10 4 virus particles, virus-like particles, or infectious units are administered.
- the optimal dosage of the viral adjuvant is less than the optimal dosage that would be administered to achieve an immune response against the immunogen if presented by or expressed by the virus or virus component in the viral adjuvant (i.e., if the virus or virus component were being used as a vaccine vector rather than as an adjuvant).
- the optimal vaccine vector dosage is about 5-fold, 10-fold, 100-fold, 1000-fold, 5,000-fold, 10,000-fold, 50,000-fold, 100, 000-fold or more greater than the optimal adjuvant dosage.
- the viral adjuvants of the invention produce an enhanced cellular immune response against an independent immunogen as compared with an immunogen presented by (e.g., on the virion surface) or expressed from the viral genomic nucleic acid that is acting as a vaccine vector.
- the cellular response can be enhanced by 1.5-fold, 2-fold, 3- fold, 4-fold, 5-fold, 10-fold, 20-fold or more.
- the dosage of immunogen can vary greatly depending on the nature and form (e.g., isolated antigen versus whole organism) of the immunogen.
- the dosage of immunogen is from about 1 ng to 500 ⁇ g, from about 0.1 ⁇ g to 100 ⁇ g, or from about 1 ⁇ g to about 50 ⁇ g.
- the dosage of immunogen is from about 0.1 ng, 1 ng, 2 ng, 5 ng, 10 ng, 25 ng, 50 ng, 100 ng, 500 ng, 1 ⁇ g, 5 ⁇ g, 10 ⁇ g, 50 ⁇ g or 100 ⁇ g and up to about 10 ng, 25 ng, 50 ng, 100 ng, 500 ng, 1 ⁇ g, 5 ⁇ g, 10 ⁇ g, 50 ⁇ g or 100 ⁇ g or more.
- the amount of immunogen administered to achieve a specified level of immune response (humoral and/or cellular and/or mucosal) in the presence (i.e., co-administration) of the viral adjuvant is reduced as compared with the amount of immunogen in the absence of the viral adjuvant, e.g., a reduction of at least about 15%, 25%, 35%, 50%, 65%, 75%, 80%, 85%, 90%, 95%, 98% or more.
- Single or multiple (i.e., booster) dosages of viral adjuvant and/or immunogen can be administered.
- the immunogen and viral adjuvant can be co-administered concurrently (e.g., within hours of each other) in the same or different composition and, in the latter case, by the same or different route.
- the viral adjuvant can be administered prior to or after administration of the immunogen (e.g., about 6, 12, 24, 36, 48, 72, 96 or 120 hours or more before or after administration of the immunogen).
- the viral adjuvant is administered before the immunogen, such that the viral adjuvant virus is present in the draining lymph node when the immunogen is subsequently administered to the subject.
- the immunogen is administered before the viral adjuvant, such that the immunogen is still present in the subject (e.g., in the draining lymph node or in the systemic circulation) when the viral adjuvant is subsequently administered to the subject.
- the viral adjuvant and immunogen can each independently be administered by any route known in the art, including administration by non- mucosal routes and mucosal routes (each as described herein).
- the immunogen and viral adjuvant can be administered to induce a mucosal immune response and/or a systemic immune response.
- the viral adjuvant (and optionally, the immunogen) is administered non-mucosally and a mucosal immune response is induced.
- Methods of non-mucosal administration are known in the art and encompass methods of delivery other than to a mucosal surface. Exemplary methods of non-mucosal administration include but are not limited to intraperitoneal, intravenous, intraarterial, intramuscular, intraventricular, intrathecal, transdermal, intradermal, subcutaneous, topical administration, and foot pad administration. *'
- Methods of mucosal administration include any method known in the art for delivering compounds 1 to a mucosal surface such as the respiratory tract, the gastrointestinal tract, the urinary tract, the reproductive tract.
- the adjuvant virus can be administered to the lungs of a subject by any suitable means, for example, administering an aerosol suspension of respirable particles, which the subject inhales.
- the respirable particles may be liquid or solid. Aerosols of liquid particles may be produced by any suitable means, such as with a pressure-driven aerosol nebulizer or an ultrasonic nebulizer, as is known to those of skill in the art. See, e.g., U.S. Patent No. 4,501 ,729. Aerosols of solid particles comprising the adjuvant virus can likewise be produced with any solid particulate medicament aerosol generator, by techniques known in the pharmaceutical art.
- mucosal administration include oral, buccal ⁇ e.g., sub-lingual), intra-tracheal, rectal, vaginal and intra-ocular administration.
- the invention further provides the viral adjuvant of the invention, compositions comprising a viral adjuvant and an immunogen (or a nucleic acid encoding an immunogen, for example, a delivery vector or a liposomal formulation), and pharmaceutical compositions comprising a viral adjuvant or a pharmaceutical composition comprising a viral adjuvant and an immunogen in a pharmaceutically acceptable excipient.
- compositions comprising a viral adjuvant and an immunogen (or a nucleic acid encoding an immunogen, for example, a delivery vector or a liposomal formulation)
- pharmaceutical compositions comprising a viral adjuvant or a pharmaceutical composition comprising a viral adjuvant and an immunogen in a pharmaceutically acceptable excipient.
- Formulation of pharmaceutical compositions is well known in the pharmaceutical arts (see, e.g., Remington's Pharmaceutical Sciences, (15th Edition, Mack Publishing Company, Easton, Pa. (1975)).
- pharmaceutically acceptable it is meant a material that is not biologically or otherwise undesirable, i.e., the material can be administered to a subject without causing any undesirable biological effects such as toxicity.
- the formulations of the invention can optionally comprise additional medicinal agents, pharmaceutical agents, carriers, buffers, adjuvants, dispersing agents, diluents, and the like.
- the viral adjuvants, and optionally an immunogen, of the invention can be formulated for administration in a pharmaceutical carrier in accordance with known techniques. See, e.g., Remington, The Science And Practice of Pharmacy (9 th Ed. 1995).
- the viral adjuvant is typically admixed with, inter alia, an acceptable carrier.
- the carrier can be a solid or a liquid, or both, and is preferably formulated as a unit-dose formulation.
- One or more viral adjuvants (and, optionally, immunogens) can be incorporated in the formulations of the invention, which can be prepared by any of the well-known techniques of pharmacy.
- the formulations of the invention include those suitable for oral, rectal, topical, buccal (e.g., sub-lingual), vaginal, intra-ocular, parenteral (e.g., subcutaneous, intramuscular including skeletal muscle, cardiac muscle, diaphragm muscle and smooth muscle, intradermal, intravenous, intraperitoneal), topical ⁇ i.e., both skin and mucosal surfaces, including airway surfaces), intranasal, transmucosal, intratracheal, transdermal, intraventricular, intraarticular, intrathecal and inhalation administration, administration to the liver by intraportal delivery, as well as direct organ injection (e.g., into the liver, into the brain for delivery to the central nervous system, into the pancreas).
- the most suitable route in any given case will depend on the nature and severity of the condition being treated and on the nature of the particular viral adjuvant which is being used.
- the carrier will typically be a liquid, such as sterile pyrogen-free water, pyrogen-free phosphate-buffered saline solution, bacteriostatic water, or Cremophor EL[R] (BASF, Parsippany, N.J.).
- the carrier can be either solid or liquid.
- the viral adjuvant can be administered in solid dosage forms, such as capsules, tablets, and powders, or in liquid dosage forms, such as elixirs, syrups, and suspensions.
- the viral adjuvant can be encapsulated in gelatin capsules together with inactive ingredients and powdered carriers, such as glucose, lactose, sucrose, mannitol, starch, cellulose or cellulose derivatives, magnesium stearate, stearic acid, sodium saccharin, talcum, magnesium carbonate and the like.
- inactive ingredients and powdered carriers such as glucose, lactose, sucrose, mannitol, starch, cellulose or cellulose derivatives, magnesium stearate, stearic acid, sodium saccharin, talcum, magnesium carbonate and the like.
- additional inactive ingredients that can be added to provide desirable color, taste, stability, buffering capacity, dispersion or other known desirable features are red iron oxide, silica gel, sodium lauryl sulfate, titanium dioxide, edible white ink and the like.
- Similar diluents can be used to make compressed tablets. Both tablets and capsules can be manufactured as sustained release products to provide for continuous release of medication over a period of
- Formulations suitable for buccal (sub-lingual) administration include lozenges comprising the viral adjuvant in a flavored base, usually sucrose and acacia or tragacanth; and pastilles comprising the viral adjuvant in an inert base such as gelatin and glycerin or sucrose and acacia.
- Formulations of the present invention suitable for parenteral administration can comprise sterile aqueous and non-aqueous injection solutions of the viral adjuvant, which preparations are generally isotonic with the blood of the intended recipient. These preparations can contain antioxidants, buffers, bacteriostats and solutes, which render the formulation isotonic with the blood of the intended recipient.
- Aqueous and non-aqueous sterile suspensions can include suspending agents and thickening agents.
- the formulations can be presented in unif ⁇ dose or multi-dose containers, for example sealed ampoules and vials, and can be stored in a freeze-dried (lyophilized) condition requiring only the addition of the sterile liquid carrier, for example, saline or water-for-injection immediately prior to use.
- Extemporaneous injection solutions and suspensions can be prepared from sterile powders, granules and tablets.
- an injectable, stable, sterile composition comprising a viral adjuvant of the invention, in a unit dosage form in a sealed container.
- the viral adjuvant is provided in the form of a lyophilizate which is capable of being reconstituted with a suitable pharmaceutically acceptable carrier to form a liquid composition suitable for injection thereof into a subject.
- Formulations suitable for rectal or vaginal administration can be presented as suppositories. These can be prepared by admixing the viral adjuvant with one or more conventional excipients or carriers, for example, cocoa butter, polyethylene glycol or a suppository wax, which are solid at room temperature, but liquid at body temperature and therefore melt in the rectum or vaginal cavity and release the viral adjuvant.
- excipients or carriers for example, cocoa butter, polyethylene glycol or a suppository wax, which are solid at room temperature, but liquid at body temperature and therefore melt in the rectum or vaginal cavity and release the viral adjuvant.
- Formulations suitable for topical application to the skin can take the form of an ointment, cream, lotion, paste, gel, spray, aerosol, or oil.
- Carriers that can be used include petroleum jelly, lanoline, polyethylene glycols, alcohols, transdermal enhancers, and combinations of two or more thereof.
- Formulations suitable for transdermal administration can be presented as discrete patches adapted to remain in intimate contact with the epidermis of the recipient for a prolonged period of time.
- Formulations suitable for transdermal administration can also be delivered by iontophoresis (see, for example, Pharmaceutical Research 3 (6):318 (1986)) and typically take the form of an optionally buffered aqueous solution of the viral adjuvant.
- Suitable formulations comprise citrate or bis ⁇ tris buffer (pH 6) or ethanol/water.
- the viral adjuvant can be formulated for nasal administration or otherwise administered to the lungs of a subject by any suitable means, for example, by an aerosol suspension of respirable particles comprising the viral adjuvant, which the subject inhales.
- the respirable particles can be liquid or solid.
- aerosol includes any gas-borne suspended phase, which is capable of being inhaled into the bronchioles or nasal passages.
- aerosol includes a gas-borne suspension of droplets, as can be produced in a metered dose inhaler or nebulizer, or in a mist sprayer. Aerosol also includes a dry powder composition suspended in air or other carrier gas, which can be delivered by insufflation from an inhaler device, for example. See Ganderton & Jones, Drug Delivery to the Respiratory Tract, Ellis Horwood (1987); Gonda (1990) Critical Reviews in Therapeutic Drug Carrier Systems 6:273-313; and Raeburn et al. (1992) J. Pharmacol. Toxicol. Methods 27:143-159.
- Aerosols of liquid particles comprising the viral adjuvant can be produced by any suitable means, such as with a pressure-driven aerosol nebulizer or an ultrasonic nebulizer, as is known to those of skill in the art. See, e.g., U.S. Patent No. 4,501 ,729.;. Aerosols of solid particles comprising the viral adjuvant can likewise be produced with any solid particulate medicament aerosol generator, by techniques known in the pharmaceutical art. Alternatively, one can administer the viral adjuvant in a local rather than systemic manner, for example, in a depot or sustained-release formulation.
- the present invention provides liposomal formulations of the viral adjuvants disclosed herein.
- the technology for forming liposomal suspensions is well known in the art.
- the viral adjuvant is an aqueous- soluble salt
- the same can be incorporated into lipid vesicles.
- the lipid layer employed can be of any conventional composition and can either contain cholesterol or can be cholesterol-free.
- the liposomes can be reduced in size, as through the use of standard sonication and homogenization techniques.
- Liposomal formulations containing the viral adjuvant can be lyophilized, alone or with immunogen, to produce a lyophilizate which can be reconstituted with a pharmaceutically acceptable carrier, such as water, to regenerate a liposomal suspension.
- a pharmaceutically acceptable carrier such as water
- VEE Venezuelan Equine Encephalitis
- VRP-GFP green fluorescent protein behind the 26S promoter
- the empty replicon particles contained an RN 1 A genome which codes for all four of the viral non-structural proteins and a 26S promoter with no transgene cloned downstream in place of the structural protein genes which have been deleted from the RNA genome (see, e.g., Push ' ko et al. (1997) Virol. 239:389-401 for a description of alphavirus replicons in which the structural protein genes have been deleted. Note that Pushko replaced the deleted structural protein genes with a transgene that is operably linked to the 26S promoter).
- these particles do not express any coding sequence downstream of the viral subgenomic promoter.
- an approximately 174 nucleotide RNA (which comprises part of the 5' leader sequence of the subgenomic transcript, a multiple cloning site, and the viral 3' UTR; this sequence has two putative open reading frames that may produce peptides of 3 and 5 amino acids in length, but not a "polypeptide of interest" as defined herein) is expressed behind the 26S promoter, which is upstream of the authentic viral 3' end (including the VEE poly A tail).
- These empty VRP were used as adjuvants in all of the experiments in which the utilized antigen was ovalbumin (OVA), Norwalk virus VLPs, or SIV gp120.
- VRP-HA virus replicon particles
- HA hemagglutinin
- mice were either mock-vaccinated and boosted in the right rear footpad or vaccinated and boosted with 10 5 infectious units (IU) of VRP expressing the hemagglutinin gene from influenza virus VRP-HA, 10 ' micrograms of dflu, or co-inoculated with 10 5 IU of VRP-GFP and 10 micrograms of dflu.
- infectious units 10 5 infectious units (IU) of VRP expressing the hemagglutinin gene from influenza virus VRP-HA, 10 ' micrograms of dflu, or co-inoculated with 10 5 IU of VRP-GFP and 10 micrograms of dflu.
- Mucosal and systemic immune responses were assessed as disclosed in Examples 3-5. • >
- IgG antibody responses in mucosal effector tissues was assessed following co-inoculation of VRP-GFP and dflu. Two weeks post-boost, animals were sacrificed and the gut lamina intestinal and nasal epithelium were harvested and allowed to incubate at 37°C for 7 days in tissue culture media. The media was subsequently analyzed for HA-specific IgG antibodies via ELISA. ELISAs were diluted 1 :40 and OD was measured (Figure 3). Experiments were repeated with similar results ( Figure 4).
- Dimeric IgA production was assessed in supernatants from the draining lymph node (DLN) following co-inoculation of VRP-GFP and dflu.
- DLN draining lymph node
- DLN draining lymph node
- contralateral lymph node contralateral lymph node
- mesenteric lymph node were harvested and allowed to incubate at 37°C for 7 days in tissue culture media. Proteins in tissue culture supernatants were separated by SDS polyacrylamide gel electrophoresis (SDS-PAGE) under non-reducing conditions and probed with an anti-lgA antibody.
- SDS-PAGE SDS polyacrylamide gel electrophoresis
- Dimeric IgA also referred to as mucosal IgA, was visible as a > 300 kDa band in mice inoculated with VRP-HA or co-inoculated with VRP-GFP and dflu ( Figure 5). No bands were visible in the dflu or mock- inoculated mice or the control blot.
- Systemic HA-Specific IgG Antibody Induction of systemic HA-specific IgG antibody responses in the spleen of mice was assessed following co- inoculation of VRP-GFP and dflu. At various time points post-boost (days 3, 7, 10, 14, 18, 21 , and 28), animals were sacrificed and the spleen was harvested and allowed to incubate at 37°C for 7 days in tissue culture media. The media was subsequently analyzed for HA-specific IgG antibodies via ELISA. Figure 6 shows the reciprocal dilution at which the OD is > 0.2.
- Systemic HA-Specific IgA Antibody Induction of systemic HA-specific IgA antibody responses in the spleen of mice was assessed following co- inoculation of VRP-GFP and dflu. At various time points post-boost (days 3, 7, 10, 14, 18, 21 , and 28), animals were sacrificed and the spleen was harvested and allowed to incubate at 37 0 C for 7 days in tissue culture media. The media was subsequently analyzed for HA-specific IgA antibodies via ELISA. Figure 7 shows the reciprocal dilution at which the OD is > two standard deviations over mock.
- Mucosal HA-Specific IgG Antibody Induction of mucosal HA-specific
- IgG antibody responses in the nasal epithelium of mice was assessed following co-inoculation of VRP-GFP and dflu.
- animals were sacrificed and the nasal epithelium was harvested and allowed to incubate at 37°C for 7 days in tissue culture media. The media was subsequently analyzed for HA-specific IgG antibodies via ELISA.
- Figure 8 shows the reciprocal dilution at which the OD is > 0.2.
- Mucosal HA-Specific IgA Antibody Induction of mucosal HA-specific IgA antibody responses in the nasal epithelium of mice was assessed following co-inoculation of VRP-GFP and dflu. At various time points post- boost (days 3, 7, 10, 14, 18, 21 , and 28), animals were sacrificed and the nasal epithelium was harvested and allowed to incubate at 37°C for 7 days in tissue culture media. The media was subsequently analyzed for HA-specific IgA antibodies via ELISA. Figure 9 shows the reciprocal dilution at which the OD is > two standard deviations over mock.
- HA-Specific IgG Antibody Production in DLN Induction of HA-specific IgG antibody responses in the DLN of mice was assessed following co- inoculation of VRP-GFP and dflu. At various time points post-boost (days 3, 7, 10, 14, 18, 21 , and 28), animals were sacrificed and the DLN was harvested and allowed to incubate at 37°C for 7 days in tissue culture media. The media was subsequently analyzed for HA-specific IgG antibodies via ELISA. Figure 10 shows the reciprocal dilution at which the OD is > 0.2.
- HA-Specific IgA Antibody Production in DLN Induction of HA-specific IgA antibody responses in the DLN of mice following co-inoculation of VRP- GFP and dflu. At various time points post-boost (days 3, 7, 10, 14, 18, 21 , and 28), animals were sacrificed and the DLN was harvested and allowed to incubate at 37°C for 7 days in tissue culture media. The media was subsequently analyzed for HA-specific IgG antibodies via ELISA. Figure 11 shows the reciprocal dilution at which the OD is > two standard deviations over mock.
- HA-Specific, Dimeric IgA Antibody Production in DLN Induction of antigen-specific, dimeric IgA in supernatants from the DLN of mice was assessed following co-inoculation of VRP-GFP and dflu. Three days post- boost, animals were sacrificed and the DLN, contralateral lymph node, and mesenteric lymph node were harvested and allowed to incubate at 37°C for 7 days in tissue culture media. Tissue culture supernatants were then bound to HA-coated ELISA plates and washed with phosphate-rbuffered saline (PBS).
- PBS phosphate-rbuffered saline
- HA-specific antibodies were then eluted from the ELISA plates, separated by SDS-PAGE under non-reducing conditions, and probed with an anti-lgA antibody.
- Dimeric IgA was visible as a > 300 kDa band in mice inoculated with VRP-HA or co-inoculated with VRP-GFP and dflu ( Figure 12).
- HA-Specific IgG and IgA Production in DLN Induction of HA-specific IgG and IgA antibodies in the DLN of mice was assessed following co- inoculation of VRP-GFP and dflu. Two weeks post-boost, animals were sacrificed and the DLN and contralateral lymph node were harvested and allowed to incubate at 37°C for 7 days in tissue culture media. The media was subsequently analyzed for HA-specific IgG and IgA antibodies via ELISA. OD determinations were conducted on 1 :40 dilutions for IgG and 1 :5 dilutions for IgA ( Figure 13).
- VRP 1 possess immune-stimulating properties which are distinct from antigen production functions, and 2) VRP are capable of serving as both systemic and mucosal adjuvants to co-inoculated antigens.
- VRP adjuvant activity is effective at inducing responses to inactivated viral particles as antigens, which present ordered, repeating antigens to the immune system.
- VRP which express a transgene in this case, GFP possess adjuvant activity.
- VEE Venezuelan Equine Encephalitis
- VRP Venezuelan Equine Encephalitis replicon particles
- Antibody titers are presented as the reciprocal dilution which results in an optical density (OD) >0.2.
- OD optical density
- VRP were capable of enhancing OVA-specific serum IgG titers by approximately 64 fold following footpad delivery, and approximately 1400 fold following nasal delivery. These data demonstrate that VRP possess intrinsic immune stimulating activity and that VRP are systemic adjuvants.
- fecal extracts were prepared from immunized animals and assayed for the presence of OVA-specific IgG and IgA antibodies.
- cholera toxin CT
- groups of eight Balb/c animals were vaccinated and boosted four weeks later in the footpad with PBS, 10 ⁇ g of Ovalbumin (OVA), 10 ⁇ g OVA co- inoculated with 1 x 10 6 I.
- OVA Ovalbumin
- U. of empty VRP (not expressing any antigen) or 10 ⁇ g OVA co-inoculated with 1 ⁇ g of CT.
- fecal extracts were prepared from immunized animals and analyzed for the presence of OVA-specific antibodies via ELISA.
- Antibody titers are presented as the reciprocal dilution which results in an optical density (OD) >0.2.
- OD optical density
- Figures 15A and 15B the levels of OVA-specific IgG and IgA antibodies present in fecal extracts following co-delivery of VRP and OVA are indistinguishable from responses induced following co-delivery of OVA and CT.
- VRP Possess Adjuvant Activity at Low Doses
- groups of eight C57BL/6 animals were vaccinated and boosted four weeks later in the footpad with PBS, 10 ⁇ g of OVA, 10 ⁇ g OVA co-inoculated with 1 x 10 2 LU. of empty VRP, 1 x 10 3 IU VRP, 1 x 10 4 IU VRP, 1 x 10 5 IU VRP, 1 x 10 6 IU VRP.
- Antibody titers are presented as the reciprocal dilution which results in an optical density (OD) >0.2.
- Figure 16 demonstrates that VRP are capable of enhancing OVA-specific serum IgG titers at doses as low as 1 x 10 2 I. U.
- ds RNA double-stranded RNA
- VRP empty VRP
- OVA-specific IgG antibodies ELISA
- animals were sacrificed and single cell suspensions were prepared from spleen and nasal epithelium, and analyzed for the presence of OVA-specific antibody-secreting-cells (ASCs) via standard ASC ELISPOT assay.
- UV treatment causes the formation of dimers in the VRP RNA, which blocks both translation of the input RNA as well as RNA replication.
- VRP are capable of enhancing both systemic and mucosal humoral immune responses following both mucosal and non-mucosal delivery in mice.
- the experiment described below was carried out to assess the ability of VRP to enhance T cell-mediated immune responses following non-mucosal delivery.
- groups of eight Balb/c animals were r- vaccinated and boosted four weeks later with PBS, 10 ⁇ g of SIV gp120 protein, 10 ⁇ g of gp120 co-inoculated with 1 x 10 6 1.
- U. ofempty VRP, or 1 x 10 6 I.
- VRP VRP expressing full length SIV gp160.
- One week post boost animals were sacrificed and spleens were isolated from immunized animals. Single cell suspensions were prepared from isolated spleens and analyzed for the presence of IFN-g-secreting cells via IFN-g ELISPOT following in vitro stimulation with either gp120 peptides or an irrelevant HA peptide. Numbers of IFN-g-secreting cells are displayed per 5 x 105 cells.
- VRP were capable of enhancing the number of gp120-specific IFN-g- secreting cells following co-delivery with gp120 protein.
- VRP humoral adjuvant activity in the experiments described above has relied on the use of HA or OVA as a test antigen.
- groups of six Balb/c animals were vaccinated and boosted four weeks later with PBS, 10 ⁇ g of Norwalk virus (NV) virus-like particles (VLP), 10 ⁇ g NV VLP co-inoculated with 1 x 10 6 I.
- PBS 10 ⁇ g of Norwalk virus (NV) virus-like particles
- VLP virus-like particles
- Norwalk virus is a member of the Norovirus family and represents a significant human pathogen. Norwalk-like viruses are responsible for numerous outbreaks of infectious gastroenteritis on cruise ships which have resulted in severe diarrhea and significant economic losses.
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US8821897B2 (en) | 2014-09-02 |
EP1773403A4 (en) | 2010-01-20 |
US20110064772A1 (en) | 2011-03-17 |
WO2006085983A3 (en) | 2007-11-08 |
EP1773403A2 (en) | 2007-04-18 |
US7862829B2 (en) | 2011-01-04 |
CA2572921A1 (en) | 2006-08-17 |
CA2572921C (en) | 2017-01-03 |
AU2005327198B2 (en) | 2011-03-31 |
EP1773403B1 (en) | 2018-04-25 |
US20080279891A1 (en) | 2008-11-13 |
AU2005327198A1 (en) | 2006-08-17 |
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