WO2006063335A2 - Automated extraction and purification of samples using optical tweezers - Google Patents
Automated extraction and purification of samples using optical tweezers Download PDFInfo
- Publication number
- WO2006063335A2 WO2006063335A2 PCT/US2005/044880 US2005044880W WO2006063335A2 WO 2006063335 A2 WO2006063335 A2 WO 2006063335A2 US 2005044880 W US2005044880 W US 2005044880W WO 2006063335 A2 WO2006063335 A2 WO 2006063335A2
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- sample
- optical
- particles
- chamber
- selected particles
- Prior art date
Links
- 238000012576 optical tweezer Methods 0.000 title claims abstract description 47
- 238000000605 extraction Methods 0.000 title claims abstract description 26
- 238000000746 purification Methods 0.000 title claims abstract description 22
- 239000002245 particle Substances 0.000 claims abstract description 73
- 238000000034 method Methods 0.000 claims abstract description 69
- 238000000920 holographic laser trapping Methods 0.000 claims abstract description 56
- 239000000872 buffer Substances 0.000 claims abstract description 42
- 238000000651 laser trapping Methods 0.000 claims abstract description 29
- 230000005484 gravity Effects 0.000 claims abstract description 8
- 238000004062 sedimentation Methods 0.000 claims abstract description 3
- 239000000523 sample Substances 0.000 claims description 123
- 210000004027 cell Anatomy 0.000 claims description 43
- 238000003384 imaging method Methods 0.000 claims description 30
- 210000002919 epithelial cell Anatomy 0.000 claims description 17
- 230000003287 optical effect Effects 0.000 claims description 17
- 239000011248 coating agent Substances 0.000 claims description 10
- 238000000576 coating method Methods 0.000 claims description 10
- 238000010926 purge Methods 0.000 claims description 9
- 230000004888 barrier function Effects 0.000 claims description 8
- 239000012530 fluid Substances 0.000 claims description 8
- 238000005286 illumination Methods 0.000 claims description 8
- 230000000007 visual effect Effects 0.000 claims description 8
- 238000000926 separation method Methods 0.000 claims description 6
- 239000000126 substance Substances 0.000 claims description 6
- 230000003068 static effect Effects 0.000 claims description 3
- 238000004140 cleaning Methods 0.000 claims 1
- 239000000463 material Substances 0.000 description 19
- 108020004414 DNA Proteins 0.000 description 17
- 239000000243 solution Substances 0.000 description 9
- 238000004458 analytical method Methods 0.000 description 8
- 108091092878 Microsatellite Proteins 0.000 description 7
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- 230000008569 process Effects 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- 239000007853 buffer solution Substances 0.000 description 3
- 238000011109 contamination Methods 0.000 description 3
- 238000012864 cross contamination Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000004744 fabric Substances 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 230000001568 sexual effect Effects 0.000 description 3
- 238000000149 argon plasma sintering Methods 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 238000012252 genetic analysis Methods 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 230000002934 lysing effect Effects 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 238000000386 microscopy Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000012521 purified sample Substances 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 239000013049 sediment Substances 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- 238000003491 array Methods 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 238000000701 chemical imaging Methods 0.000 description 1
- 239000006059 cover glass Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000000799 fluorescence microscopy Methods 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 238000012632 fluorescent imaging Methods 0.000 description 1
- 238000001215 fluorescent labelling Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000001093 holography Methods 0.000 description 1
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- 230000002452 interceptive effect Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
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- 238000010030 laminating Methods 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000004973 liquid crystal related substance Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 210000000947 motile cell Anatomy 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 239000013307 optical fiber Substances 0.000 description 1
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- 210000000582 semen Anatomy 0.000 description 1
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- 230000036962 time dependent Effects 0.000 description 1
- 238000002834 transmittance Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/32—Micromanipulators structurally combined with microscopes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
-
- G01N15/1433—
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B5/00—Optical elements other than lenses
- G02B5/32—Holograms used as optical elements
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
-
- G01N15/149—
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N2001/002—Devices for supplying or distributing samples to an analysing apparatus
- G01N2001/007—Devices specially adapted for forensic samples, e.g. tamper-proofing, sample tracking
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Electro-optical investigation, e.g. flow cytometers
- G01N15/1434—Electro-optical investigation, e.g. flow cytometers using an analyser being characterised by its optical arrangement
- G01N2015/1454—Electro-optical investigation, e.g. flow cytometers using an analyser being characterised by its optical arrangement using phase shift or interference, e.g. for improving contrast
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
- Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
Landscapes
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Optics & Photonics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
Claims
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP05853728A EP1838866A4 (en) | 2004-12-10 | 2005-12-12 | Automated extraction and purification of samples using optical tweezers |
JP2007545705A JP4904286B2 (en) | 2004-12-10 | 2005-12-12 | Method and apparatus for automatic extraction and purification of samples using optical tweezers |
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US63516404P | 2004-12-10 | 2004-12-10 | |
US60/635,164 | 2004-12-10 | ||
US63498004P | 2004-12-13 | 2004-12-13 | |
US60/634,980 | 2004-12-13 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2006063335A2 true WO2006063335A2 (en) | 2006-06-15 |
WO2006063335A3 WO2006063335A3 (en) | 2009-04-16 |
Family
ID=36578664
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2005/044880 WO2006063335A2 (en) | 2004-12-10 | 2005-12-12 | Automated extraction and purification of samples using optical tweezers |
Country Status (5)
Country | Link |
---|---|
US (2) | US7875845B2 (en) |
EP (2) | EP1838866A4 (en) |
JP (1) | JP4904286B2 (en) |
TW (1) | TW200632099A (en) |
WO (1) | WO2006063335A2 (en) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2008199919A (en) * | 2007-02-16 | 2008-09-04 | Kyushu Univ | Method and apparatus for separating and collecting cell |
DE102008060332A1 (en) * | 2008-12-03 | 2010-06-10 | Albert-Ludwigs-Universität Freiburg | Microfluidic sorting device with optical tweezers |
JP2010538644A (en) * | 2007-09-13 | 2010-12-16 | アリックス インク | Method and apparatus for screening objects in forensic DNA analysis and medical diagnosis |
JP2010538645A (en) * | 2007-09-11 | 2010-12-16 | アリックス インク | Combined method and apparatus for sorting objects |
Families Citing this family (18)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2010144814A2 (en) * | 2009-06-13 | 2010-12-16 | Arryx, Inc. | Particle sorting using fluid streams |
US20110177547A1 (en) * | 2004-12-10 | 2011-07-21 | Arryx, Inc. | Particle Sorting Using Fluid Streams |
US8169600B2 (en) | 2006-09-15 | 2012-05-01 | Arryx, Inc. | Surface mapping by optical manipulation of particles in relation to a functionalized surface |
GB0618605D0 (en) * | 2006-09-21 | 2006-11-01 | Univ St Andrews | Optical sorting |
GB0813090D0 (en) * | 2008-07-17 | 2008-08-27 | Univ St Andrews | Optical trap |
WO2010101671A1 (en) | 2009-01-16 | 2010-09-10 | New York University | Automated real-time particle characterization and three-dimensional velocimetry with holographic video microscopy |
CN102753954A (en) | 2009-12-22 | 2012-10-24 | 纽约大学 | Sorting colloidal particles into multiple channels with optical forces: prismatic optical fractionation |
WO2012037642A1 (en) | 2010-08-20 | 2012-03-29 | Marksmen Cellject Inc. | System and method for automated sperm manipulation |
US8723104B2 (en) * | 2012-09-13 | 2014-05-13 | City University Of Hong Kong | Methods and means for manipulating particles |
ES2913524T3 (en) | 2014-11-12 | 2022-06-02 | Univ New York | Colloidal fingerprinting for soft materials using total holographic characterization |
DE102015116391B4 (en) | 2015-09-28 | 2017-04-27 | Marion Vollmer | Medical device for the selective separation of a biological sample |
EP3414517B1 (en) | 2016-02-08 | 2021-10-06 | New York University | Holographic characterization of protein aggregates |
US10611995B2 (en) * | 2018-08-15 | 2020-04-07 | Deepcell, Inc. | Systems and methods for particle analysis |
CN110331125B (en) * | 2019-06-28 | 2023-04-07 | 南京航空航天大学 | Method for automatically realizing pairing of multiple groups of cells |
KR102266834B1 (en) * | 2019-08-13 | 2021-06-17 | 경희대학교 산학협력단 | Microfluidic device for manipulating sperm |
US11543338B2 (en) | 2019-10-25 | 2023-01-03 | New York University | Holographic characterization of irregular particles |
US11948302B2 (en) | 2020-03-09 | 2024-04-02 | New York University | Automated holographic video microscopy assay |
CN116013575B (en) * | 2022-12-21 | 2023-08-22 | 深圳大学 | Low-temperature optical heating tweezers control system and method based on water abnormal expansion characteristics |
Family Cites Families (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA1311404C (en) | 1987-03-13 | 1992-12-15 | Kenneth H. Kortright | Automated analyzer and method for screening cells or formed bodies for enumeration of populations expressing selected characteristics |
US5392155A (en) | 1988-06-17 | 1995-02-21 | Tamari; Vladimir F. | De-diffraction methods |
US6005964A (en) | 1996-01-24 | 1999-12-21 | The Board Of Trustees Of The University Of Illinois | Automatic machine vision microscope slide inspection system and method |
US6055106A (en) * | 1998-02-03 | 2000-04-25 | Arch Development Corporation | Apparatus for applying optical gradient forces |
DE19952322C2 (en) | 1999-10-29 | 2002-06-13 | Evotec Ag | Method and device for particle separation |
US20020108859A1 (en) * | 2000-11-13 | 2002-08-15 | Genoptix | Methods for modifying interaction between dielectric particles and surfaces |
US20020121443A1 (en) * | 2000-11-13 | 2002-09-05 | Genoptix | Methods for the combined electrical and optical identification, characterization and/or sorting of particles |
GB2371858B (en) * | 2001-02-05 | 2004-10-13 | Abb Offshore Systems Ltd | Monitoring particles in a fluid flow |
WO2003008943A1 (en) * | 2001-07-19 | 2003-01-30 | Tufts University | Optical array device and methods of use thereof for screening, analysis and manipulation of particles |
US7150834B2 (en) * | 2003-07-31 | 2006-12-19 | Arryx, Inc. | Multiple laminar flow-based rate zonal or isopycnic separation with holographic optical trapping of blood cells and other static components |
EP2806427A3 (en) | 2002-07-31 | 2015-04-22 | Premium Genetics (UK) Limited | System and method of sorting materials using holographic laser steering |
DE10313760B3 (en) | 2003-03-27 | 2004-06-03 | Fresenius Kabi Deutschland Gmbh | Connector for a bag containing medical fluids, for e.g. transfusion/infusion, has a connector with a clamp section integrated into the package by an expanded base and without a connection tube |
-
2005
- 2005-12-12 WO PCT/US2005/044880 patent/WO2006063335A2/en active Application Filing
- 2005-12-12 EP EP05853728A patent/EP1838866A4/en not_active Withdrawn
- 2005-12-12 US US11/298,565 patent/US7875845B2/en not_active Expired - Fee Related
- 2005-12-12 EP EP12182488A patent/EP2573543A2/en not_active Withdrawn
- 2005-12-12 TW TW094143876A patent/TW200632099A/en unknown
- 2005-12-12 JP JP2007545705A patent/JP4904286B2/en not_active Expired - Fee Related
-
2010
- 2010-12-15 US US12/926,888 patent/US20110091932A1/en not_active Abandoned
Non-Patent Citations (2)
Title |
---|
None |
See also references of EP1838866A4 |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2008199919A (en) * | 2007-02-16 | 2008-09-04 | Kyushu Univ | Method and apparatus for separating and collecting cell |
JP2010538645A (en) * | 2007-09-11 | 2010-12-16 | アリックス インク | Combined method and apparatus for sorting objects |
JP2010538644A (en) * | 2007-09-13 | 2010-12-16 | アリックス インク | Method and apparatus for screening objects in forensic DNA analysis and medical diagnosis |
DE102008060332A1 (en) * | 2008-12-03 | 2010-06-10 | Albert-Ludwigs-Universität Freiburg | Microfluidic sorting device with optical tweezers |
WO2010063478A1 (en) * | 2008-12-03 | 2010-06-10 | Albert-Ludwigs-Universität Freiburg | Microfluidic sorting apparatus with optical tweezers |
DE102008060332B4 (en) * | 2008-12-03 | 2013-01-10 | Albert-Ludwigs-Universität Freiburg | Method for sorting at least one particle with a microfluidic sorting device with optical tweezers |
Also Published As
Publication number | Publication date |
---|---|
EP1838866A4 (en) | 2010-04-14 |
US20110091932A1 (en) | 2011-04-21 |
JP4904286B2 (en) | 2012-03-28 |
JP2008530981A (en) | 2008-08-14 |
US7875845B2 (en) | 2011-01-25 |
EP1838866A2 (en) | 2007-10-03 |
WO2006063335A3 (en) | 2009-04-16 |
EP2573543A2 (en) | 2013-03-27 |
TW200632099A (en) | 2006-09-16 |
US20060134603A1 (en) | 2006-06-22 |
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