WO2006061417A2 - Macrocyclic quinazole derivatives and their use as mtki - Google Patents

Macrocyclic quinazole derivatives and their use as mtki Download PDF

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Publication number
WO2006061417A2
WO2006061417A2 PCT/EP2005/056609 EP2005056609W WO2006061417A2 WO 2006061417 A2 WO2006061417 A2 WO 2006061417A2 EP 2005056609 W EP2005056609 W EP 2005056609W WO 2006061417 A2 WO2006061417 A2 WO 2006061417A2
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WIPO (PCT)
Prior art keywords
alkyl
het
alkyloxy
hydroxy
optionally substituted
Prior art date
Application number
PCT/EP2005/056609
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French (fr)
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WO2006061417A3 (en
Inventor
Eddy Jean Edgard Freyne
Marc Willems
Peter Ten Holte
Alexandra Papanikos
Werner Constant Johan Embrechts
Pierre Henri Storck
Virginie Sophie Poncelet
Original Assignee
Janssen Pharmaceutica N.V.
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Priority to EA200701233A priority Critical patent/EA013995B1/en
Priority to KR1020077013133A priority patent/KR101320431B1/en
Priority to NZ555496A priority patent/NZ555496A/en
Priority to JP2007544918A priority patent/JP5140431B2/en
Priority to EP05826362.5A priority patent/EP1828201B1/en
Priority to ES05826362.5T priority patent/ES2559305T3/en
Priority to CN2005800420435A priority patent/CN101072781B/en
Priority to BRPI0518394A priority patent/BRPI0518394B8/en
Priority to AU2005313350A priority patent/AU2005313350B2/en
Priority to US11/720,693 priority patent/US20100152174A1/en
Application filed by Janssen Pharmaceutica N.V. filed Critical Janssen Pharmaceutica N.V.
Priority to CA2588764A priority patent/CA2588764C/en
Priority to DK05826362.5T priority patent/DK1828201T3/en
Priority to SI200532035T priority patent/SI1828201T1/en
Priority to MX2007006820A priority patent/MX2007006820A/en
Publication of WO2006061417A2 publication Critical patent/WO2006061417A2/en
Publication of WO2006061417A3 publication Critical patent/WO2006061417A3/en
Priority to IL183705A priority patent/IL183705A/en
Priority to NO20073426A priority patent/NO339891B1/en
Priority to HK08102117.6A priority patent/HK1111416A1/en
Priority to US14/285,008 priority patent/US9688691B2/en
Priority to US15/601,014 priority patent/US10208062B2/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D498/00—Heterocyclic compounds containing in the condensed system at least one hetero ring having nitrogen and oxygen atoms as the only ring hetero atoms
    • C07D498/02—Heterocyclic compounds containing in the condensed system at least one hetero ring having nitrogen and oxygen atoms as the only ring hetero atoms in which the condensed system contains two hetero rings
    • C07D498/04—Ortho-condensed systems
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D498/00—Heterocyclic compounds containing in the condensed system at least one hetero ring having nitrogen and oxygen atoms as the only ring hetero atoms
    • C07D498/02—Heterocyclic compounds containing in the condensed system at least one hetero ring having nitrogen and oxygen atoms as the only ring hetero atoms in which the condensed system contains two hetero rings
    • C07D498/06—Peri-condensed systems
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
    • A61K31/505—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim
    • A61K31/529—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim forming part of bridged ring systems
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D487/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00
    • C07D487/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00 in which the condensed system contains two hetero rings
    • C07D487/04—Ortho-condensed systems
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D498/00—Heterocyclic compounds containing in the condensed system at least one hetero ring having nitrogen and oxygen atoms as the only ring hetero atoms
    • C07D498/02—Heterocyclic compounds containing in the condensed system at least one hetero ring having nitrogen and oxygen atoms as the only ring hetero atoms in which the condensed system contains two hetero rings
    • C07D498/08—Bridged systems

Definitions

  • the human genome encompasses some 2,000 proteins that utilize adenosine 5'- triphosphate (ATP) in one way or another and some 500 of these encode for protein kinases, i.e the protein-tyrosine and protein-serine/threonine kinases, that share a catalytic domain conserved in sequence and structure but which are notably different in how their catalysis is regulated.
  • Substrate phosphorylation by these enzymes is nature's predominant molecular way of organizing cellular signal transduction and regulating biochemical processes in general. It is not surprising, therefore, that abnormal phosphorylation of cellular proteins is a hallmark of disease and that there is a growing interest in the use of kinase inhibitors as drugs for therapeutic intervention in many disease states such as cancer, diabetes, inflammation and arthritis.
  • kinase inhibitors that are quinazoline derived macrocycles, hereinafter also referred to as multi targeting kinase inhibitors (MTKI), found to possess anti-proliferative activity, such as anti-cancer activity and which are accordingly useful in methods of treatment of the human or animal body, for example in the manufacture of medicaments for use in hyper proliferative disorders such as atherosclerosis, restenosis and cancer.
  • MTKI multi targeting kinase inhibitors
  • the invention also relates to processes for the manufacture of said quinazoline derivatives, to pharmaceutical compositions containing them and to their use in the manufacture of medicaments of use in the production of anti-proliferative effect .
  • tyrosine kinase enzymes also called tyrosine kinases.
  • Tyrosine kinases are a class of enzymes, which catalyse the transfer of the terminal phosphate of adenosine triphosphate to the phenolic hydroxy- group of a tyrosine residue present in the target protein. It is known, that several oncogenes, involved in the transformation of a cell into a malignant tumour cell, encode tyrosine kinase enzymes including certain growth factor receptors such as EGF, FGF, IGF-IR, IR, PDGF and VEGF.
  • This family of receptor tyrosine kinases and in particular the EGF family of receptor tyrosine kinases are frequently present in common human cancers such as breast cancer, non-small cell lung cancers including adenocarcinomas and squamous cell cancer of the lung, bladder cancer, oesophageal cancer, gastrointestinal cancer such as colon, rectal or stomach cancer, cancer of the prostate, leukaemia and ovarian, bronchial or pancreatic cancer, which are examples of cell proliferation disorders. Accordingly, it has been recognised that the selective inhibition of tyrosine kinases will be of value in the treatment of cell proliferation related disorders.
  • Herceptin® Trastuzumab
  • GleevecTM imatinib mesylate
  • Herceptin ® is targeted against He ⁇ 2/neu, a receptor tyrosine kinase found to be amplified up to 100-fold in about 30% of patients with invasive breast cancer.
  • Herceptin ® (Trastuzumab) proved to have anti-tumour activity against breast cancer (Review by L.K.
  • GleevecTM imatinib mesylate
  • BcR-AbI abelson tyrosine kinase
  • GleevecTM imatinib mesylate
  • imatinib mesylate showed a spectacular efficacy with minimal side effects that led to an approval within 3 months of submission.
  • the speed of passage of this agent through clinical trials and regulatory review has become a case study in rapid drug development (Drucker BJ. & Lydon N., "Lessons learned from the development of an AbI tyrosine kinase inhibitor for chronic myelogenous leukaemia.”, 2000, J.Clin. Invest. 105, 3).
  • EGF receptor tyrosine kinase inhibitors specifically attenuates the growth in athymic nude mice of transplanted carcinomas such as human mammary carcinoma or human squamous cell carcinoma (Review by T.R. Burke Jr., Drugs of the Future, 1992, 17, 119).
  • EGF receptor tyrosine kinase inhibitors specifically attenuates the growth in athymic nude mice of transplanted carcinomas such as human mammary carcinoma or human squamous cell carcinoma.
  • ErbituxTM also called C225, Cetuximab
  • EGF receptor tyrosine kinases are shown to be implicated in non-malignant proliferative disorders such as psoriasis (Elder et al., Science, 1989, 243; 811). It is therefore expected that inhibitors of EGF type receptor tyrosine kinases will be useful in the treatment of non-malignant diseases of excessive cellular proliferation such as psoriasis, benign prostatic hypertrophy, atherosclerosis and restenosis.
  • This invention concerns compounds of formula (I)
  • Z represents NH
  • Y represents -C 3 - 9 alkyl-, -C 2 . 9 alkenyl-, -C 1-5 alkyl-oxy-C 1-5 alkyl-,
  • X 2 represents a direct bond
  • R 1 represents hydrogen, cyano, halo or hydroxy, preferably halo
  • R 2 represents hydrogen, cyano, halo, hydroxy, hydroxycarbonyl-, C 1-4 alkyloxycarbonyl-, Het 16 -carbonyl-, Ci_ 4 alkyl-, C 2 . 6 alkynyl-, Ar 5 , Het 1 or dihydroxyborane
  • R 3 represents hydrogen, cyano, halo, hydroxy, formyl, C 1-6 alkoxy-, C ⁇ ⁇ alkyl-,
  • R 3 represents C 1-4 alkyl substituted with one or where possible two or more substituents selected from hydroxy or halo
  • R 4 represents Ar 4 -C 1-4 alkyloxy-, C 1-4 alkyloxy- or R 4 represents C 1-4 alkyloxy substituted with one or where possible two or more substituents selected from hydroxy-, halo,
  • C ⁇ alkylcarbonyl optionally substituted with one or more substituents selected from C 1-4 alkylsulfonyl, hydroxy- and C ⁇ alkyloxy-; or
  • R 8 represents C ⁇ 4 alkyl substituted with one or more substituents selected from
  • R 9 represents hydrogen or C 1-4 alkyl-;
  • R 10 represents Het 4 or C 1-4 alkyl- substituted with C ⁇ alkylsulfonyl-, ;
  • R 11 represents hydrogen, C 1-4 alkyl- or C 1-4 alkyl-oxy-carbonyl-;
  • R 12 represents hydrogen, C 1-4 alkyl-, C 1-6 alkyloxycarbonyl- or C 1-6 alkyloxycarbonyl- substituted with phenyl;
  • R 13 represents hydrogen, Het ⁇ C ⁇ alkyl, C]. 6 alkyloxycarbonyl optionally substituted with phenyl or R 13 represents Ar 6 -sulfonyl or Het 24 -C 1 . 4 alkylcarbonyl; in particular morpholinyl-C ⁇ alkyl; R 14 and R 15 are each independently selected from hydrogen, d- 4 alkyl, Het 15 -d. 4 alkyl- or d_ 4 a]ky]oxyd. 4 a]ky]-;
  • R 16 and R 17 each independently represents hydrogen, d- 4 alkyl or d_ 4 alkyl substituted with hydroxy-, C 3 . 6 cycloalkyl or phenyl; or R 16 and R 17 taken together with the carbon atom to which they are attached form a C 3-6 cycloalkyl;
  • R 18 represents hydrogen or d_ 4 alkyl optionally substituted with hydroxy or phenyl
  • R 19 represents hydrogen or C 1-4 alkyl, in particular hydrogen or methyl, even more particular hydrogen
  • R 20 represents hydrogen or C 1-4 alkyl, in particular hydrogen or methyl;
  • R 21 represents hydrogen, Ci -4 alkyl, Het 23 -C 1-4 alkylcarbonyl- or
  • R 21 represents mono-or di(Ci -4 alkyr)amino-d- 4 alkyl-carbonyl- optionally substituted with hydroxy, pyrimidinyl, dimethylamine or d_ 4 alkyloxy;
  • R 22 represents hydrogen or Ci. 4 alkyl optionally substituted with hydroxy or
  • Ci- 4 alkyloxy represents C 1-4 alkyl optionally substituted with hydroxy-, Ci- 4 alkyloxy- or Het 25 ; R 23 may also represent hydrogen when R 16 and R 17 taken together with the carbon atom to which they are attached form a C 3-6 cycloalkyl;
  • R 25 and R 26 each independently represent hydrogen, C 1-4 alkyl, Ci -4 alkylsulfonyl-, aminocarbonyl-, mono- or di(d_ 4 alkyl)aminocarbonyl-, C 1-4 alkylcarbonyl-, C 1-4 alkyloxycarbonyl- or d.
  • R 25 and R 26 each independently represent hydrogen, C 1-4 alkyl, C 1-4 alkylsulfonyl-, aminocarbonyl-, mono- or di(Ci -4 alkyl)aminocarbonyl- or d_ 4 alkylcarbonyl-;
  • R 27 and R 28 each independently represent hydrogen, Ci -4 alkyl, C 1-4 alkylsulfonyl-, aminocarbonyl-, mono- or diCd ⁇ alky ⁇ aminocarbonyl-, Ci -4 alkylcarbonyl-,
  • R 29 and R 30 each independently represent hydrogen, aminosulfonyl, aminocarbonyl, mono- or di(C 1-4 alkyl)aminocarbonyl-, mono- or di(Ci. 4 alkyl)aminosulfonyl-, or
  • Ci_ 4 alkyl- optionally substituted with one or more substituents selected from NR 31 R 32 , C 1-4 alkylsulfonyl, aminocarbonyloxy-, hydroxy-, d -4 alkyloxy-, aminocarbonyl- and mono- or di(Ci -4 alkyl)aminocarbonyl-, or
  • C].4alkyloxycarbonyl optionally substituted with one or more substituents selected from hydroxy, C 1-4 alkyloxy- and C 1-4 alkylsulfonyl-, or
  • C 1-4 alkylcarbonyl optionally substituted with one or more substituents selected from hydroxy-, C 1-4 alkyloxy- and C 1 _ 4 alkylsulfonyl-;
  • R 31 and R 32 each independently represent hydrogen, C 1-4 alkyl, C 1-4 alkylsulfonyl-, aminocarbonyl-, mono- or di(C 1-4 alkyl)aminocarbonyl-, C 1-4 alkylcarbonyl-,
  • R 33 represents hydrogen or C 1-4 alkyl;
  • R 34 represents Ci_ 4 alkylsulfonyl-, aminocarbonyl-, mono- or di(C 1-4 alkyl)aminocarbonyl-, C 1 . 4 alkylcarbonyl-, C 1-4 alkyloxycarbonyl- or
  • R 35 represents hydrogen or C 1-4 alkyl
  • R 36 represents C ⁇ alkylsulfonyl-, aminocarbonyl-, mono- or di(C 1-4 alkyl)aminocarbonyl-, C ⁇ alkylcarbonyl-, C 1-4 alkyloxycarbonyl- or
  • R 37 and R 38 each independently represent hydrogen, Ci ⁇ alkyl, C]- 4 alkylsulfonyl-, Het 12 or C 1-4 alkyl substituted with one or more substituents selected from
  • R 39 and R 40 each independently represent aminosulfonyl, aminocarbonyl, mono- or di(C 1-4 alkyl)aminocarbonyl-, mono- or di(C]- 4 alkyl)aminosulfonyl-, or C 1-4 alkyl- substituted with one or more substituents selected from NR 31 R 32 ,
  • C 1-4 alkyloxycarbonyl optionally substituted with one or more substituents selected from hydroxy-, C].
  • 4 alkyloxy- and C 1-4 alkylsulfonyl-, or C 1-4 alkylcarbonyl optionally substituted with one or more substituents selected from hydroxy-, Q ⁇ alkyloxy- and C 1-4 alkylsulfonyl-;
  • Het 1 represents thiazolyl or 2-bora-l,3-dioxolanyl wherein said Het 1 is optionally substituted with one or where possible two, three, four or more substituents selected from amino, C 1-4 alkyl, hydroxy-C 1-4 alkyl-, phenyl, phenyl-C].
  • Het 2 represents a heterocycle selected from tetrahydropyranyl, tetrahydrofuranyl, furanyl, 1,1-dioxothiomorpholinyl, piperazininonyl, tetrahydro-l,l-dioxido-2H- thiopyranyl, piperidinonyl, azetidinyl or 2-azetidinonyl wherein said Het 2 is optionally substituted with one or where possible two or more substituents selected from hydroxy, amino, NR 29 R 30 , aminocarbonyl, mono- or di(C 1-4 alkyl)aminocarbonyl, C 1-4 alkylsulfonyl or Ci_ 4 alkyl- optionally substituted with one or more substituents selected from
  • C 1-4 alkyloxycarbonyl optionally substituted with one or more substituents selected from hydroxy, C 1-4 alkyloxy- and Ci -4 alkylsulfonyl-, or
  • Het 2 represents a heterocycle selected from morpholinyl, piperazinyl, piperidinyl, pyrrolidinyl, thiomorpholinyl or 1,1-dioxothiomorpholinyl wherein said Het 2 is optionally substituted with one or where possible two or more substituents selected from
  • NR 27 R 28 C 1-4 alkylsulfonyl, aminocarbonyloxy-, aminocarbonyl- and mono- or dKC ⁇ alkyOaminocarbonyl-, or Ci- 4 alkyloxy- optionally substituted with Ci -4 alkyloxy-, or
  • C ⁇ alkyloxycarbonyl optionally substituted with one or more substituents selected from hydroxy, C 1-4 alkyloxy- and C 1-4 alkylsulfonyl-, or
  • Het 3 represents a heterocycle selected from tetrahydropyranyl, tetrahydrofuranyl, furanyl, 1,1-dioxothiomorpholinyl, piperazininonyl, tetrahydro-l,l-dioxido-2H- thiopyranyl, piperidinonyl, azetidinyl or 2-azetidinonyl wherein said Het 3 is optionally substituted with one or where possible two or more substituents hydroxy-, amino, C].
  • Het 4 represents a heterocycle selected from morpholinyl, piperazinyl, piperidinyl, furanyl, pyrazolyl, dioxolanyl, thiazolyl, oxazolyl, imidazolyl, isoxazolyl, oxadiazolyl, pyridinyl or pyrrolidinyl wherein said Het 4 is substituted with one or where possible two or more substituents selected from Ci_ 4 alkyl-sulfonyl- C 1-4 alkyl-, C ⁇ alkyloxy- optionally substituted with Ci -4 alkyloxy- or hydroxy;
  • Het 5 represents a heterocycle selected from furanyl, piperazinyl,
  • Het 6 and Het 7 each independently represents a heterocycle selected from piperazinyl, piperidinyl or pyrrolidinyl wherein said heterocycles are optionally substituted with one or more substituents selected from hydroxy-, amino, hydroxy-Ci -4 alkyl-, C 1-4 alkyloxy-C 1-4 alkyl- and C ⁇ alkyl-;
  • Het 8 represents a heterocycle selected from tetrahydropyranyl, tetrahydrofuranyl, 1 , 1 -dioxothiomorpholinyl, piperazininonyl, tetrahydro- 1 , l-dioxido-2H- thiopyranyl, piperidinonyl, azetidinyl or 2-azetidinonyl wherein said Het 8 is optionally substituted with aminosulfonyl, aminocarbonyl, mono- or d ⁇ Q ⁇ alkytyaminocarbonyl-, mono- or
  • Ci -4 alkylcarbonyl optionally substituted with one or more substituents selected from hydroxy, C 1-4 alkyloxy- and Ci_ 4 alkylsulfonyl-; or
  • Het 8 represents a heterocycle selected from furanyl, piperidinyl or piperazinyl wherein said Het 8 is substituted with aminocarbonyl, mono- or di(C 1-4 alkyl)aminocarbonyl-, mono- or di(C 1-4 alkyl)aminosulfonyl-, or
  • Ci_ 4 alkylsulfonyl aminocarbonyloxy-, hydroxy-, C 1-4 alkyloxy-, aminocarbonyl- and mono- or di(Ci -4 alkyl)aminocarbonyl-, or Ci -4 alkyloxycarbonyl optionally substituted with one or more substituents selected from hydroxy, Ci -4 alkyloxy- and Ci ⁇ alkylsulfonyl-, or
  • Het 9 and Het 10 each independently represents a heterocycle selected from piperazinyl, piperidinyl or pyrrolidinyl wherein said heterocycles are optionally substituted with one or more substituents selected from hydroxy-, amino, hydroxy-Ci -4 alkyl-, C 1-4 alkyloxy-C 1-4 alkyl- and C ⁇ alkyl-;
  • Het 11 represents 2-imidazolidinonyl- or ⁇ ° ;
  • Het 12 represents a heterocycle selected from morpholinyl, piperazinyl, piperidinyl or pyrrolidinyl wherein said Het 12 is optionally substituted with one or where possible two or more substituents selected from hydroxy, amino or C ⁇ alkyl-; Het 13 represents a heterocycle selected from furanyl, piperazinyl, 1,1-dioxothiomorpholinyl, piperazininonyl, piperidinyl, tetrahydro-l,l-dioxido-
  • Het 14 and Het 15 each independently represent a heterocycle selected from morpholinyl, piperazinyl, piperidinyl or pyrrolidinyl wherein said Het 14 and Het 15 are optionally substituted with one or where possible two or more substituents selected from hydroxy, amino or C 1-4 alkyl;
  • Het 16 represents a heterocycle selected from piperidinyl or pyrrolidinyl
  • Het 23 and Het 25 each independently represents a heterocycle selected from morpholinyl, pyrrolidinyl, piperazinyl or piperidinyl wherein said Het 23 is optionally substituted with one or where possible two or more substituents selected from C 1-4 alkyl, C 3-6 cycloalkyl, hydroxy-C ⁇ alkyl-, C ⁇ alkyloxyCi ⁇ alkyl or polyhydroxy- C 1-4 alkyl-;
  • Het 24 represents morpholinyl, pyrrolidinyl, piperazinyl or piperidinyl;
  • Ar 4 , Ar 5 or Ar 6 each independently represent phenyl optionally substituted with nitro, cyano, C 1-4 alkylsulfonyl-, Ci_ 4 alkylsulfonylamino-, aminosulfonylamino-, hydroxy-C 1-4 alkyl, aminosulfonyl-, hydroxy-, C 1-4 alkyloxy- or C 1-4 alkyl, preferably
  • Ar 4 or Ar 5 each independently represent phenyl optionally substituted with cyano; further characterised in that either
  • Y represents -C 1-2 alkyl-NR 23 -CO-CR 16 R 17 -NH-;
  • Het 1 represents 2-bora-l,3-dioxolanyl optionally substituted with one or where possible two, three, four or more substituents selected from amino, C h alky], hydroxy-Ci ⁇ alkyl-, phenyl, phenyl-C 1-4 alkyl-, Ci -4 alkyl-oxy-Ci- 4 alkyl-, mono- or di(Ci -4 alkyl)amino- or amino-carbonyl-; R 13 represents Ci ⁇ alkyloxycarbonyl optionally substituted with phenyl or R 13 represents Ar 6 -sulfonyl or Het 24 -C 1-4 alkylcarbonyl; or R 4 represents Ci_ 4 alkyloxy substituted with at least one substituent selected from
  • Ci -4 alkyloxycarbonyl mono- or di(Ci -4 alkyl)aminocarbonyl-; mono- or di(Ci -4 alkyl)aminocarbonyl substituted with Ci -4 alkylsulfonyl-; or
  • C 1-4 alkylcarbonyl optionally substituted with one or more substituents selected from C] -4 alkylsulfonyl, hydroxy- and Ci -4 alkyloxy-; or R 8 represents Ci -4 alkyl substituted with one or more substituents selected from hydroxy Ci -4 alkylsulfonyl-, NR 25 R 26 , aminocarbonyloxy-, C 1-4 alkylcarbonyloxy-, aminocarbonyl-, C ]-4 alkyloxy-C 1-4 alkyloxy-, and
  • Het 13 represents Q- ⁇ alkyloxycarbonyl optionally substituted with phenyl or R 13 represents Ar 6 -sulfonyl or Het 24 -Ci -4 alkylcarbonyl; in particular morpholinyl-C 1-4 alkyl; and Het 2 represents a heterocycle selected from morpholinyl, piperazinyl, piperidinyl, pyrrolidinyl or thiomorpholinyl said Het 2 substituted with one or where possible two or more substituents selected from C 1-4 alkyl- substituted with one or more substituents selected from NR 27 R 28 , Ci -4 alkylsulfonyl, aminocarbonyloxy-, aminocarbonyl- and mono- or di(Ci_ 4 alkyl)aminocarbonyl-; or
  • Ci_ 4 alkyloxycarbonyl optionally substituted with one or more substituents selected from hydroxy, Ci_ 4 alkyloxy- and C].
  • 4 alkylsulfonyl-; or C ⁇ alkylcarbonyl optionally substituted with one or more substituents selected from hydroxy-, Ci -4 alkyloxy- and C 1-4 alkylsulfonyl-.
  • - halo is generic to fluoro, chloro, bromo and iodo
  • - C 1 2 alkyl defines methyl or ethyl
  • C 1 3 alkyl defines straight and branched chain saturated hydrocarbon radicals having from 1 to 3 carbon atoms such as, for example, methyl, ethyl, propyl and the like;
  • - C 1 4 alkyl defines straight and branched chain saturated hydrocarbon radicals having from 1 to 4 carbon atoms such as, for example, methyl, ethyl, propyl, butyl, 1-methylethyl, 2-methylpropyl, 2,2-dimethylethyl and the like;
  • - C j.g alkyl defines straight and branched chain saturated hydrocarbon radicals having from 1 to 5 carbon atoms such as, for example, methyl, ethyl, propyl, butyl, pentyl, 1-methylbutyl, 2,2-dimethylpropyl, 2,2-dimethylethyl and the like;
  • - C ⁇ alkyl is meant to include C 1-5 alkyl and the higher homologues thereof having 6 carbon atoms such as, for example hexyl, 1,2-dimethylbutyl, 2-methylpentyl and the like;
  • - C 1 7 alkyl is meant to include Ci -6 alkyl and the higher homologues thereof having 7 carbon atoms such as, for example 1,2,3-dimethylbutyl, 1, 2-methylpentyl and the like;
  • - C 3 _ 9 alkyl defines straight and branched chain saturated hydrocarbon radicals having from 3 to 9 carbon atoms such as propyl, butyl, pentyl, hexyl, heptyl, octyl, nonyl and the like;
  • alkenyl defines straight and branched chain hydrocarbon radicals containing one double bond and having from 2 to 4 carbon atoms such as, for example vinyl, 2-propenyl, 3-butenyl, 2-butenyl and the like;
  • - C 3 _ 9 alkenyl defines straight and branched chain hydrocarbon radicals containing one double bond and having from 3 to 9 carbon atoms such as, for example 2-propenyl, 3-butenyl, 2-butenyl, 2-pentenyl, 3-pentenyl, 3-methyl-2-butenyl, 3-hexenyl and the like;
  • - C 2-6 alkynyl defines straight and branched chain hydrocarbon radicals containing one triple bond and having from 2 to 6 carbon atoms such as, for example, 2-propynyl, 3-butynyl, 2-butynyl, 2-pentynyl, 3-pentynyl, 3-methyl-2-butynyl, 3-hexynyl and the like;
  • - C 3 _ 6 cycloalkyl is generic to cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl;
  • - Ci_ 4 alkyloxy defines straight or branched saturated hydrocarbon radicals such as methoxy, ethoxy, propyloxy, butyloxy, 1-methylethyloxy, 2-methylpropyloxy and the like;
  • Ci_ 6 alkyloxy is meant to include Ci ⁇ alkyloxy and the higher homologues such as methoxy, ethoxy, propyl oxy, butyl oxy, 1-methylethyloxy, 2-methylpropyloxy and the like;
  • - polyhydroxy-C 1-4 alkyl is generic to a C ⁇ alkyl as defined hereinbefore, having two, three or where possible more hydroxy substituents, such as for example trifluoromethyl.
  • said radical is attached with the carbon atom to the R 3 , R 4 bearing cyclic moiety of the compounds of formula (I)
  • said radical is attached with the carbon atom to the R 1 , R 2 bearing phenyl moiety of the compounds of formula (I).
  • pyrrolyl also includes 2H-pyrrolyl; triazolyl includes 1,2,4-triazolyl and 1,3,4-triazolyl; oxadiazolyl includes 1,2,3-oxadiazolyl, 1,2,4-oxadiazolyl, 1,2,5-oxadiazolyl and 1,3,4-oxadiazolyl; thiadiazolyl includes 1,2,3-thiadiazolyl, 1,2,4-thiadiazolyl, 1,2,5-thiadiazolyl and 1,3,4-thiadiazolyl; pyranyl includes 2H-pyranyl and 4H-pyranyl.
  • heterocycles as mentioned in the above definitions and hereinafter may be attached to the remainder of the molecule of formula (I) through any ring carbon or heteroatom as appropriate.
  • the heterocycle when it is imidazolyl, it may be a 1-imidazolyl, 2-imidazolyl, 3-imidazolyl, 4-imidazolyl and 5-imidazolyl; when it is thiazolyl, it may be 2-thiazolyl, 4-thiazolyl and 5-thiazolyl; when it is triazolyl, it may be 1,2,4-triazol-l-yl, l,2,4-triazol-3-yl, l,2,4-triazol-5-yl, 1,3,4-triazol- 1-yl and l,3,4-triazol-2-yl; when it is benzothiazolyl, it may be 2-benzothiazolyl, 4-benzothiazolyl, 5-benzothiazolyl, 6-benzothiazolyl and 7-benz
  • the pharmaceutically acceptable addition salts as mentioned hereinabove are meant to comprise the therapeutically active non-toxic acid addition salt forms which the compounds of formula (I) are able to form.
  • the latter can conveniently be obtained by treating the base form with such appropriate acid.
  • Appropriate acids comprise, for example, inorganic acids such as hydrohalic acids, e.g. hydrochloric or hydrobromic acid; sulfuric; nitric; phosphoric and the like acids; or organic acids such as, for example, acetic, propanoic, hydroxyacetic, lactic, pyruvic, oxalic, malonic, succinic (i.e.
  • butane-dioic acid maleic, fumaric, malic, tartaric, citric, methanesulfonic, ethanesulfonic, benzenesulfonic, p-toluenesulfonic, cyclamic, salicylic, ⁇ -aminosalicylic, pamoic and the like acids.
  • the pharmaceutically acceptable addition salts as mentioned hereinabove are meant to comprise the therapeutically active non-toxic base addition salt forms which the compounds of formula (I) are able to form.
  • base addition salt forms are, for example, the sodium, potassium, calcium salts, and also the salts with pharmaceutically acceptable amines such as, for example, ammonia, alkylamines, benzathine, N-methyl-D-glucamine, hydrabamine, amino acids, e.g. arginine, lysine.
  • salt forms can be converted by treatment with an appropriate base or acid into the free acid or base form.
  • addition salt as used hereinabove also comprises the solvates which the compounds of formula (I) as well as the salts thereof, are able to form.
  • solvates are for example hydrates, alcoholates and the like.
  • stereochemically isomeric forms as used hereinbefore defines the possible different isomeric as well as conformational forms which the compounds of formula (I) may possess.
  • chemical designation of compounds denotes the mixture of all possible stereochemically and conformationally isomeric forms, said mixtures containing all diastereomers, enantiomers and/or conformers of the basic molecular structure.
  • All stereochemically isomeric forms of the compounds of formula (I) both in pure form or in admixture with each other are intended to be embraced within the scope of the present invention.
  • N-oxide forms of the compounds of formula (I) are meant to comprise those compounds of formula (I) wherein one or several nitrogen atoms are oxidized to the so-called iV-oxide.
  • a first group of compounds according to the present invention consists of those compounds of formula (I) wherein one or more of the following restrictions apply; Z represents NH; Y represents -C 3 . 9 alkyl-, -C 2 _ 9 alkenyl-, -Ci-salkyl-oxy-Ci.salkyl-, -Ci-ealkyl-NH-CO-, -NH-CO-Ci- ⁇ alkyl-, -CO-Ci_ 7 alkyl-, -C 1-7 alkyl-CO-, Ci. 6 alkyl-CO-Ci_ 6 alkyl, -Ci.
  • X 2 represents a direct bond, -0-, -O-Ci -2 alkyl, -CO-Ci -2 alkyl- or NR 12 -C 1-2 alkyl-;
  • R 1 represents hydrogen, cyano, halo or hydroxy, preferably halo
  • R 2 represents hydrogen, cyano, halo, hydroxy, hydroxycarbonyl-, Ci_ 4 alkyloxycarbonyl-, Het 16 -carbonyl-, C 1-4 alkyl-, C 2-6 alkynyl-, Ar 5 , Het 1 or dihydroxyborane ;
  • R 3 represents hydrogen, cyano, halo, hydroxy, formyl, C 1-6 alkoxy-, Ci -6 alkyl-,
  • Ci- ⁇ alkoxy- substituted with halo, or R 3 represents d_ 4 alkyl substituted with one or where possible two or more substituents selected from hydroxy or halo;
  • R 4 represents Ar 4 -C 1-4 alkyloxy-, Ci -4 alkyloxy- or R 4 represents Ci- 4 alkyloxy substituted with one or where possible two or more substituents selected from hydroxy-, halo, Ci_ 4 alkyloxy-, C 1-4 alkyloxy-C 1-4 alkyloxy-, NR 37 R 38 -carbonyloxy-, Het 5 - carbonyloxy-, NR 7 R 8 , NR 9 R 10 -carbonyl-, Het 3 -carbonyl-, Het 13 -oxy- or Het 2 -;
  • R 7 represents hydrogen or C 1-4 alkyl
  • R 8 represents C 3-6 cycloalkyl, Het 6 -carbonyl-, Het 7 -aminocarbonyl-, Het 8 ,
  • Ci alkylsulfonyl-, NR R , aminocarbonyloxy-, aminocarbonyl-, d. 4 alyloxy-d- 4 alkyloxy-, and Het 11 ;
  • R 9 represents hydrogen or Ci_ 4 alkyl-
  • R 10 represents Het 4 or Ci_ 4 alkyl- substituted with Ci -4 alkylsulfonyl-, ;
  • R 11 represents hydrogen, Ci -4 alkyl- or Ci- 4 alkyl-oxy-carbonyl-;
  • R 12 represents hydrogen, Ci -4 alkyl-, d ⁇ alkyloxycarbonyl- or Ci_ 6 alkyloxycarbonyl- substituted with phenyl;
  • R 13 represents hydrogen, Het 14 -C]. 4 alkyl, optionally substituted with phenyl or R 13 represents Ar 6 -sulfonyl or Het 24 -C]_ 4 alkylcarbonyl; in particular morpholinyl-C 1-4 alkyl;
  • R 14 and R 15 are each independently selected from hydrogen, Ci -4 alkyl, Het 15 -C 1-4 alkyl- or C 1 _ 4 alkyloxyC 1 . 4 alkyl-;;
  • R 16 and R 17 each independently represents hydrogen, C 1-4 alkyl or C]_ 4 alkyl substituted with hydroxy- or phenyl; or R 16 and R 17 taken together with the carbon atom to which they are attached form a C 3-6 C ycloalkyl;
  • R 18 represents hydrogen or C 1-4 alkyl optionally substituted with hydroxy or phenyl
  • R 19 represents hydrogen or C 1-4 alkyl, in particular hydrogen or methyl, even more particular hydrogen
  • R 20 represents hydrogen or Ci -4 alkyl, in particular hydrogen or methyl;
  • R 21 represents hydrogen, C 1-4 alkyl, Het 23 -C 1-4 alkylcarbonyl- or
  • R 21 represents mono-or di(C 1-4 alkyl)amino-C 1-4 alkyl-carbonyl- optionally substituted with hydroxy, pyrimidinyl, dimethylamine or C 1 4 alkyloxy;
  • R 22 represents hydrogen or C 1-4 alkyl optionally substituted with hydroxy or
  • R 23 represents C]. 4 alkyl optionally substituted with hydroxy-, C 1-4 alkyloxy- or Het 23 ;
  • R 23 may also represent hydrogen when R 16 and R 17 taken together with the carbon atom to which they are attached form a C 3-6 cycloalkyl;
  • R 25 and R 26 each independently represent hydrogen, C 1-4 alkyl, C 1 4 alkylsulfonyl-, aminocarbonyl-, mono- or di(C 1-4 alkyl)aminocarbonyl-, C]- 4 alkylcarbonyl-,
  • R 25 and R 26 each independently represent hydrogen, C 1-4 alkyl, C 1-4 alkylsulfonyl-, aminocarbonyl-, mono- or di(C 1-4 alkyl)aminocarbonyl- or C 1-4 alkylcarbonyl-;
  • R 27 and R 28 each independently represent hydrogen, C ⁇ alkyl, C]. 4 alkylsulfonyl-, aminocarbonyl-, mono- or di(C 1-4 alkyl)aminocarbonyl-, C 1-4 alkylcarbonyl-,
  • R 29 and R 30 each independently represent hydrogen, aminosulfonyl, aminocarbonyl, mono- or di(C 1-4 alkyl)aminocarbonyl-, mono- or di(C]. 4 alkyl)aminosulfonyl-, or
  • R 31 and R 32 each independently represent hydrogen, d.
  • R 33 represents hydrogen or C 1-4 alkyl
  • R 34 represents Ci -4 alkylsulfonyl-, aminocarbonyl-, mono- or di(C ⁇ 4 alkyl)aminocarbonyl-, C 1-4 alkylcarbonyl-, C 1-4 alkyloxycarbonyl- or C 1-4 alkyl substituted with one or more substituents selected from C 1-4 alkylsulfonyl-
  • R 35 represents hydrogen or C 1-4 alkyl
  • R 36 represents C 1-4 alkylsulfonyl-, aminocarbonyl-, mono- or di(Ci -4 alkyl)aminocarbonyl-, C 1-4 alkylcarbonyl-, Ci -4 alkyloxycarbonyl- or Ci. 4 alkyl substituted with one or more substituents selected from C 1-4 alkylsulfonyl-
  • R 37 and R 38 each independently represent hydrogen, d_ 4 alkyl, C 1-4 alkylsulfonyl-, Het 12 or C 1-4 alkyl substituted with one or more substituents selected from Ci- 4 alkylsulfonyl-, hydroxy- and C 1-4 alkyloxy-;
  • R 39 and R 40 each independently represent aminosulfonyl, aminocarbonyl, mono- or di(C 1-4 alkyl)aminocarbonyl-, mono- or di(C 1-4 alkyl)aminosulfonyl-, or
  • Ci_ 4 alkyloxycarbonyl optionally substituted with one or more substituents selected from hydroxy, Ci -4 alkyloxy- and Ci -4 alkylsulfonyl-, or
  • Ci ⁇ alkylcarbonyl optionally substituted with one or more substituents selected from hydroxy-, Ci -4 alkyloxy- and C 1-4 alkylsulfonyl-;
  • Het 1 represents thiazolyl or 2-bora-l,3-dioxolanyl wherein said Het 1 is optionally substituted with one or where possible two, three, four or more substituents selected from amino, Ci -4 alkyl, hydroxy-C 1-4 alkyl-, phenyl, phenyl-d ⁇ alkyl-, d- 4 alkyl-oxy-d_ 4 alkyl-, mono- or di(d- 4 alkyl)amino- or amino-carbonyl-;
  • Het 2 represents a heterocycle selected from tetrahydropyranyl, tetrahydrofuranyl, furanyl, 1,1-dioxothiomorpholinyl, piperazininonyl, tetrahydro-l,l-dioxido-2H- thiopyranyl, piperidinonyl, azetidinyl or 2-azetidinonyl wherein said Het 2 is optionally substituted with
  • NR 27 R 28 C 1-4 alkylsulfonyl, aminocarbonyloxy-, aminocarbonyl- and mono- or di(C 1-4 alkyl)aminocarbonyl-, or Ci_ 4 alkyloxy- optionally substituted with C 1-4 alkyloxy-, or
  • C 1-4 alkyloxycarbonyl optionally substituted with one or more substituents selected from hydroxy, Ci_ 4 alkyloxy- and Ci -4 alkylsulfonyl-, or
  • Het 2 represents a heterocycle selected from morpholinyl, piperazinyl, piperidinyl or pyrrolidinyl wherein said mo ⁇ holinyl, piperazinyl, piperidinyl or pyrrolidinyl are optionally substituted with one or where possible two or more substituents selected from
  • Ci -4 alkyl- optionally substituted with one or more substituents selected from NR 27 R 28 , Ci -4 alkylsulfonyl, aminocarbonyloxy-, aminocarbonyl- and mono- or di(Ci -4 alkyl)aminocarbonyl-, or
  • Het 3 represents a heterocycle selected from tetrahydropyranyl, tetrahydrofuranyl, furanyl, 1,1-dioxothiomorpholinyl, piperazininonyl, tetrahydro-l,l-dioxido-2H- thiopyranyl, piperidinonyl, azetidinyl or 2-azetidinonyl wherein said Het 3 is optionally substituted with one or where possible two or more substituents hydroxy-, amino, C 1-4 alkyl-, C ⁇ cycloalkyl-Ci ⁇ al
  • Het 3 represents a heterocycle selected from mo ⁇ holinyl, piperazinyl, piperidinyl or pyrrolidinyl wherein said Het 3 is optionally substituted with one or where possible two or more substituents selected from NR 35 R 36 , C 1-4 alkyl-sulfonyl-C 1 - 4 alkyl- or
  • Het 4 represents a heterocycle selected from mo ⁇ holinyl, piperazinyl, piperidinyl, furanyl, pyrazolyl, dioxolanyl, thiazolyl, oxazolyl, imidazolyl, isoxazolyl, oxadiazolyl, pyridinyl or pyrrolidinyl wherein said Het 4 is substituted with one or where possible two or more substituents selected from Ci -4 alkyl-sulfonyl- Ci_ 4 alkyl-, Ci -4 alkyloxy- optionally substituted with Ci -4 alkyloxy- or hydroxy
  • Het 5 represents a heterocycle selected from furanyl, piperazinyl,
  • Het 6 and Het 7 each independently represents a heterocycle selected from piperazinyl, piperidinyl or pyrrolidinyl wherein said heterocycles are optionally substituted with one or more substituents selected from hydroxy-, amino-, hydroxy-C 1-4 alkyl-,
  • Het represents a heterocycle selected from tetrahydropyranyl, tetrahydrofuranyl, 1 , 1 -dioxothiomorpholinyl, piperazininonyl, tetrahydro- 1 , 1 -dioxido-2H- thiopyranyl, piperidinonyl, azetidinyl or 2-azetidinonyl wherein said Het 8 is optionally substituted with aminosulfonyl, aminocarbonyl, mono- or di(Ci -4 alkyl)aminocarbonyl-, mono- or di(C].
  • Het represents a heterocycle selected from furanyl, piperidinyl or piperazinyl wherein said Het 8 is substituted with aminocarbonyl, mono- or di(C 1-4 alkyl)aminocarbonyl-, mono- or di(C]- 4 alkyl)aminosulfonyl-, or C 1-4 alkyl- substituted with one or more substituents selected from NR 33 R 34 , C].
  • Het 11 represents 2-imidazolidinonyl- or ⁇ ⁇ ;
  • Het 12 represents a heterocycle selected from morpholinyl, piperazinyl, piperidinyl or pyrrolidinyl wherein said Het 12 is optionally substituted with one or where possible two or more substituents selected from hydroxy-, amino or C ⁇ alkyl-;
  • Het 13 represents a heterocycle selected from furanyl, piperazinyl, 1,1- dioxothiomorpholinyl, piperazininonyl, piperidinyl, tetrahydro-l,l-dioxido-2H- thiopyranyl, piperidinonyl, morpholinyl, piperazinyl or pyrrolidinyl
  • Het 16 represents a heterocycle selected from piperidinyl or pyrrolidinyl;
  • Ar 4 , Ar 5 or Ar 6 each independently represent phenyl optionally substituted with nitro, cyano, C 1-4 alkylsulfonyl-, C 1-4 alkylsulfonylamino-, aminosulfonylamino-, hydroxy-C 1-4 alkyl, aminosulfonyl-, hydroxy-, C ⁇ alkyloxy- or C 1-4 alkyl, preferably Ar 4 or Ar 5 each independently represent phenyl optionally substituted with cyano; further characterised in that either Y represents -C 1-2 alkyl-NR 23 -CO-CR 16 R 17 -NH-;
  • Het 1 represents 2-bora-l,3-dioxolanyl optionally substituted with one or where possible two, three, four or more substituents selected from amino, C 1-4 alkyl, hydroxy-C 1-4 alkyl-, phenyl, phenyl-C 1-4 alkyl-, C 1-4 alkyl-oxy-C 1-4 alkyl-, mono- or di(C 1-4 alkyl)amino- or amino-carbonyl-;
  • R 13 represents C 1-6 alkyloxycarbonyl optionally substituted with phenyl or R 13 represents Ar 6 -sulfonyl or Het 24 -C 1-4 alkylcarbonyl; or
  • R 4 represents C ⁇ alkyloxy substituted with at least one substituent selected from C 1-4 alkyloxy-C ! _ 4 alkyloxy-, NR R -carbonyloxy-, Het -carbonyloxy-, NR 7 R 8 , NR 9 R 10 -carbonyl-, Het 3 -carbonyl-, Het 13 -oxy- or Het 2 -.
  • Another group of compounds according to the present invention consists of those compounds of formula (I) wherein one or more of the following restrictions apply; Z represents NH;
  • Y represents -C 3-9 alkyl-, -C 1-5 alkyl-NR 13 -C 1-5 alkyl-, -d- 5 alkyl-NR 14 -CO-C 1-5 alkyl-, -d_ 6 alkyl-CO-NH-, -d-ealkyl-NH-CO-, -C ⁇ alkyl-NR ⁇ -CO-CR ⁇ R ⁇ -NH-,
  • alkyl-NH-CO-Het 20 - X 1 represents a direct bond, O, -O-d_ 2 alkyl-, NR 11 , or -NR ⁇ -C 1-2 alkyl-;
  • X 2 represents a direct bond, -C 1-2 alkyl-, CO-C 1-2 alkyl or NR 12 -C 1-2 alkyl-; in particular
  • X 2 represents a direct bond, -C 1-2 alkyl- or NR 12 -C 1-2 alkyl-;
  • R 1 represents hydrogen, cyano, halo or hydroxy;
  • R 2 represents hydrogen, halo, cyano, C 2-6 alkynyl, hydroxy, hydroxycarbonyl, d_ 4 alkyloxycarbonyl- or Het 1 ; in particular R 2 represents hydrogen, halo, cyano, acetylene (-C ⁇ CH), hydroxy, hydroxycarbonyl, C 1-4 alkyloxycarbonyl- or Het 1 ; more in particular R 2 represents hydrogen, halo, cyano, hydroxy, hydroxycarbonyl,
  • R 3 represents hydrogen, cyano, halo, hydroxy, formyl, C 1-6 alkyloxy or C 1-6 alkyloxy- substituted with halo;
  • R 4 represents Ar 4 -d-4alkyloxy, C 1-4 alkyloxy-, or C 1-4 alkyloxy- substituted with one or where possible two or more substituents selected from hydroxy, d- 4 alkyloxy-,
  • R 4 represents
  • R 7 represents hydrogen, hydroxyC ⁇ alkyl- or C 1-4 alkyl
  • R 8 represents C 1-4 alkyloxycarbonyl or C 1-4 alkyl- substituted with one or more substituents selected from C 1-4 alkylsulfonyl-, C ⁇ alkylcarbonyloxy Or NR 25 R 26 ; in particular R represents C 1-4 alkyl- substituted with one or more substituents selected from C 1-4 alkylsulfonyl- or NR 25 R 26
  • R 11 represents hydrogen, C 1-4 alkyloxycarbonyl or C ⁇ alkyl; in particular R 11 represents hydrogen or C ⁇ alkyl
  • R 12 represents hydrogen or C 1-4 alkyl
  • R 13 represents d ⁇ alkyloxycarbonyl optionally substituted with phenyl or R 13 represents Ar 6 -sulfonyl or Het 24 -d_ 4 alkylcarbonyl
  • R 14 and R 15 each independently represent hydrogen or C 1-4 alkyl; in particular R 14 and
  • R 15 each independently represent hydrogen;
  • R 16 and R 17 each independently represent hydrogen or C 1-4 alkyl optionally substituted with C 3 _ 6 cycloalkyl or R 16 and R 17 taken together with the carbon atom to which they are attached form a C 3 . 6 cycloalkyl; in a particular embodiment R 16 and R 17 taken together with the carbon atom to which they are attached form a
  • R 21 represents hydrogen or C 1-4 alkyloxycarbonyl; in particular R 21 represents
  • R 23 represents C]- 4 alkyl optionally substituted with hydroxy-, C 1-4 alkyloxy- or Het 25 ;
  • R 23 may also represent hydrogen when R 16 and R 17 taken together with the carbon atom to which they are attached form a C 3-6 C ycloalkyl;
  • R 25 and R 26 each independently represent hydrogen, C 1-4 alkyl, C 1-4 alkylsulfonyl,
  • R 25 and R 26 each independently represents hydrogen or Ci -4 alkylcarbonyl;
  • R and R each independently represent hydrogen, C 1-4 alkyl, C 1-4 alkylsulfonyl, C]. 4 alkyloxycarbonyl or C ⁇ alkylc arbonyl; in particular R 27 and R 28 each independently represent hydrogen or C]- 4 alkylcarbonyl; Het 1 represents 2-bora-l,3-dioxolanyl- optionally substituted with one or where possible two, three, four or more substituents selected from amino, C 1-4 alkyl, hydroxy-C 1-4 alkyl-, phenyl, phenyl-C 1-4 alkyl, C 1-4 alkyloxyC 1-4 alkyl-, mono- or di(C 1-4 alkyl)amino- or aminocarbonyl-; Het 2 represents 1,1-dioxothiomorpholinyl optionally substituted with
  • Het 2 represents piperidinyl or piperazinyl substituted with C 1-4 alkyloxycarbonyl or -C 1-4 alkyl-NR 27 R 28 ;
  • Het 20 represents pyrrolidinyl, 2-pyrrolidinonyl, piperidinyl or hydroxy-pyrrolidinyl; in particular Het 20 represents pyrrolidinyl, piperidinyl or hydroxy-pyrrolidinyl; more in particular Het 20 represents pyrrolidinyl;
  • Het 25 represents a heterocycle selected from morpholinyl or piperazinyl wherein said heterocycle is optionally substituted with C 1-4 alkyl, hydroxy-Ci ⁇ alkyl,
  • Ar 4 , Ar 5 or Ar 6 each independently represents phenyl optionally substituted with nitro, cyano, hydroxy, hydroxyd- 4 alkyl, C 1-4 alkyl or C 1-4 alkyloxy; further characterised in that either Y represents -C 1-2 alkyl-NR 23 -CO-CR 16 R 17 -NH-; or
  • R 4 represents C ⁇ alkyloxy substituted with at least one substituent selected from Ci_ 4 alkyloxy-C M alkyloxy-, NR 7 R 8 or Het 2 .
  • Another group of compounds according to the present invention consists of those compounds of formula (I) wherein one or more of the following restrictions apply; Z represents NH;
  • Y represents -C 3-9 alkyl-, -Ci_ 5 alkyl-NR 13 -C 1-5 alkyl-, -Ci -5 alkyl-NR 14 -CO-Ci. 5 alkyl-, -Ci-ealkyl-CO-NH-, -Ci.ealkyl-NH-CO-, -Ci -2 alkyl-NR 23 -CO-CR 16 R 17 -NH-,
  • C 1-3 alkyl-NH-CO-Het 20 - X 1 represents a direct bond, O, -O-C 1-2 alkyl-, NR 1 ⁇ or -NR u -C 1-2 alkyl-;
  • X 2 represents a direct bond, -C ⁇ alkyl-, CO-Ci_ 2 alkyl or NR 12 -C 1-2 alkyl-; in particular
  • X 2 represents a direct bond, -Ci -2 alkyl- or NR 12 -Ci -2 alkyl-;
  • R 1 represents hydrogen or halo;
  • R 2 represents hydrogen, halo, C 2 _ 6 alkynyl, cyano or Het 1 ; in particular R 2 represents hydrogen, halo, C2- 6 alkynyl or Het 1 ; more in particular R 2 represents hydrogen, halo, acetylene or Het 1 ; or R 2 represents hydrogen, halo, cyano or Het 1 ; R 3 represents hydrogen; R 4 represents Ar 4 -C 1 _ 4 alkyloxy, C 1-4 alkyloxy-, or C 1-4 alkyloxy- substituted with one or where possible two or more substituents selected from hydroxy, C 1-4 alkyloxy-, C 1-4 alkyloxy-Ci -4 alkyloxy, NR 7 R 8 or Het 2 ; in particular R 4 represents
  • Ci -4 alkyloxy or NR 7 R 8 R 7 represents hydrogen or Ci_ 4 alkyl;
  • R 8 represents Ci- 4 alkyloxycarbonyl or C 1-4 alkyl- substituted with one or more substituents selected from C 1-4 alkylsulfonyl-, hydroxy, C ⁇ alkylcarbonyloxy or
  • R 8 represents Ci- 4 alkyl- substituted with one or more substituents selected from C 1-4 alkylsulfonyl- or NR 25 R 26 ;
  • R 11 represents hydrogen or C 1-4 alkyl;
  • R 12 represents hydrogen or Ci -4 alkyl;
  • R 13 represents Ar 6 -sulfonyl or Ci -6 alkyloxycarbonyl optionally substituted with phenyl;
  • R 14 and R 15 represent hydrogen
  • R 16 and R 17 each independently represent hydrogen or Ci -4 alkyl optionally substituted with C 3 . 6 cycloalkyl or R 16 and R 17 taken together with the carbon atom to which they are attached form a C 3 . 6 cycloalkyl; in a particular embodiment R 16 and R 17 taken together with the carbon atom to which they are attached form a
  • R 21 represents hydrogen or C 1-4 alkyloxycarbonyl
  • R 23 represents Ci 4 alkyl optionally substituted with hydroxy-, C ⁇ alkyloxy- or Het 25 ; R 23 may also represent hydrogen when R 16 and R 17 taken together with the carbon atom to which they are attached form a C 3-6 cycloalkyl; R 25 and R 26 each independently represent hydrogen or C 1-4 alkylcarbonyl; R 27 and R 28 each independently represent hydrogen or Ci- 4 alkylcarbonyl; Het 1 represents 2-bora-l,3-dioxolanyl-; Het 2 represents 1,1-dioxothiomorpholinyl, piperidinyl or piperazinyl wherein said Het 2 is optionally substituted with C 1-4 alkyloxycarbonyl or -C 1-4 alkyl-NR 27 R 28 ; Het 20 represents pyrrolidinyl;
  • Het 25 represents a heterocycle selected from morpholinyl or piperazinyl wherein said heterocycle is optionally substituted with C 1-4 alkyl, hydroxy-C 1-4 alkyl, C ⁇ ⁇ alkyloxy-C ⁇ ⁇ alkyl or polyhydroxy-C 1-4 alkyl;
  • Ar 4 represents phenyl;
  • Ar 5 represents phenyl; or
  • Ar 6 represents phenyl optionally substituted with nitro; further characterised in that either
  • Y represents -C 1-2 alkyl-NR 23 -CO-CR 16 R 17 -NH-;
  • R 4 represents d- 4 alkyloxy substituted with at least one substituent selected from Ci ⁇ alkyloxy-C ⁇ alkyloxy-, NR 7 R 8 or Het 2 ; in particular C ⁇ alkyloxy substituted with C ⁇ alkyloxy-C ⁇ alkyloxy- or NR 7 R 8 .
  • Y represents -C 3-9 alkyl-, -C 1-5 alkyl-NR 13 -C 1-5 alkyl-, -C 1-5 alkyl-NR 14 -CO-C 1-5 alkyl-, -C 1-2 alkyl-NR 21 -H 2 -CO-NH-C 1-3 alkyl- or -C 1-2 alkyl-NR 23 -CO-CR 16 R 17 -NH-; in particular Y represents -C 3-9 alkyl-, -C 1-5 alkyl-NR 13 -C 1-5 alkyl- or -Ci_ 2 alkyl-NR 23 -CO-CR 16 R 17 -NH- X 1 represents O or -O-Ci_ 2 alkyl-; inparticular X 1 represents O
  • X 2 represents a direct bond, C ⁇ alkyl, -CO-C 1-2 alkyl or NR 12 -Ci_ 2 alkyl; in particular X 2 represents a direct bond or NR 12 -C 1-2 alkyl-;
  • R 1 represents hydrogen or halo; in particular R 1 represents hydrogen; R 2 represents halo, C 2 _ 6 alkynyl, cyano or Het 1 ; in particular R 2 represents halo, acetylene or Het 1 ; more in particular R 2 represents halo or Het 1 ;
  • R 3 represents hydrogen;
  • R 4 represents Ar 4 -Ci_ 4 alkyloxy-, C ⁇ alkyloxy- or Ci_ 4 alkyloxy substituted with one or where possible two or more substituents selected from Het 2 , NR 7 R 8 , hydroxy and Ci_ 4 alkyloxy-Ci_ 4 alkyloxy-; in particular R 4 represents Ar 4 -C 1-4 alkyloxy-, Ci -4 alkyloxy- or Ci_ 4 alkyloxy substituted with Ci- 4 alkyloxy-C 1-4 alkyloxy-; R 7 represents hydrogen or C 1-4 alkyl;
  • R 8 represents C ⁇ alkyl substituted with NR 25 R 26 or C 1-4 alkylsulfonyl
  • R 12 represents hydrogen or C 1-4 alkyl-
  • R 13 represents Ar 6 -sulfonyl or C 1-6 alkyloxycarbonyl optionally substituted with phenyl;
  • R 16 and R 17 represents hydrogen, C 1-4 alkyl or R 16 and R 17 taken together with the carbon atom to which they are attached from a C 3 _ 6 cycloalkyl;
  • R 23 represents hydrogen or C 1-4 alkyl; in particular R 23 represents Ci_ 4 alkyl and R 23 represents hydrogen when R 16 and R 17 taken together with the carbon atom to which they are attached from a C 3-6 cycloalkyl;
  • R 25 and R 26 each independently represent hydrogen or C 1-4 alkylcarbonyl
  • R 27 and R 28 each independently represent hydrogen or C 1-4 alkylcarbonyl
  • Het 1 represents 2-bora-l,3-dioxolanyl
  • Het 2 represents piperidinyl, piperazinyl, morpholinyl, thiomorpholinyl or
  • Het 2 is optionally substituted with d- 4 alkyloxycarbonyl or NR 27 R 28 -C 1-4 alkyl; in particular Het 2 represents 1,1-dioxothiomorpholinyl; piperidinyl substituted with C 1-4 alkyloxycarbonyl; or piperazinyl substituted with Q ⁇ alkyloxycarbonyl or NR 27 R 28 -C ! _ 4 alkyl-;
  • Ar 4 represents phenyl
  • Ar 5 represents phenyl
  • Ar 6 represents phenyl optionally substituted with nitro.
  • Z represents NH
  • Y represents -C 3-9 alkyl-,-C 1-5 alkyl-NR 13 -C 1-5 alkyl-, -C 1-5 alkyl-NR 14 -CO-C 1-5 alkyl-, -C 1 _ 2 alkyl-NR 21 -CH 2 -CO-NH-C 1-3 alkyl-, C 1-6 alkyl -NH-CO- or -d_ 2 alkyl-NR 23 -
  • X 1 represents O or -O-C ⁇ alkyl-; in particular X 1 represents O X 2 represents a direct bond, C ⁇ alkyl, -CO-C ⁇ alkyl or NR 12 -C 1-2 alkyl; in particular X 2 represents -CO-C ⁇ alkyl or NR 12 -C ! _ 2 alkyl-; R 1 represents hydrogen, cyano or halo; in particular R 1 represents hydrogen or halo, more in particular R 1 represents hydrogen, fluoro or bromo;
  • R 2 represents halo, C 2-6 alkynyl, cyano or Het 1 ; in particular R 2 represents halo, acetylene or Het 1 ; more in particular R 2 represents halo or Het 1 ; R 3 represents hydrogen;
  • R 4 represents Ar 4 -Ci -4 alkyloxy-, C ⁇ alkyloxy- or C 1-4 alkyloxy substituted with one or where possible two or more substituents selected from Het 2 , NR 7 R 8 , hydroxy and C 1-4 alkyloxy-Ci -4 alkyloxy-; in particular R 4 represents Ar 4 -C 1-4 alkyloxy-, C 1-4 alkyloxy- or Ci- 4 alkyloxy substituted with one or where possible two or more substituents selected from Het 2 , NR 7 R 8 or hydroxy;
  • R 7 represents hydrogen, hydroxy-C 1-4 alkyl- or C 1-4 alkyl
  • R 8 represents Ci -4 alkylcarbonyl , C 1-4 alkyloxycarbonyl or C ⁇ alkyl substituted with hydroxy-C 1-4 alkyloxy-, NR 25 R 26 , Ci -4 alkylcarbonyloxy- or C 1-4 alkylsulfonyl;
  • R 12 represents hydrogen or C 1-4 alkyl-;
  • R 13 represents Ar 6 -sulfonyl or d. 6 alkyloxycarbonyl optionally substituted with phenyl;
  • R 16 and R 17 each independently represents hydrogen, d_ 4 alkyl or R 16 and R 17 taken together with the carbon atom to which they are attached from a C 3-6 cycloalkyl;
  • R 23 represents C ⁇ alkyl optionally substituted with Het 25 ;
  • R 23 may also represent hydrogen when R 16 and R 17 taken together with the carbon atom to which they are attached form a C 3 _ 6 cycloalkyl;
  • R 25 and R 26 each independently represent hydrogen or C 1-4 alkylcarbonyl
  • R 27 and R 28 each independently represent hydrogen or C 1-4 alkylcarbonyl
  • Het 1 represents 2-bora-l,3-dioxolanyl
  • Het 2 represents piped dinyl, piperazinyl, morpholinyl, thiomorpholinyl or 1,1 -dioxothiomorpholinyl wherein said Het 2 is optionally substituted with
  • Ci -4 alkyloxycarbonyl or NR 27 R 28 -Ci- 4 alkyl in particular Het 2 represents 1,1 -dioxothiomorpholinyl; piperidinyl substituted with Ci -4 alkyloxycarbonyl; or piperazinyl substituted with Ci -4 alkyloxycarbonyl or NR 27 R 28 -Ci- 4 alkyl-;
  • Het 25 represents morpholinyl
  • Ar 4 represents phenyl
  • Ar 5 represents phenyl
  • Ar 6 represents phenyl optionally substituted with nitro.
  • a further interesting group of compounds consists of those compounds of formula (I) wherein one or more of the following restrictions apply : Z represents NH; Y represents -C 3 . 9 alkyl-,-C 1-5 alkyl-NR 13 -C ⁇ 5 alkyl-,
  • X 1 represents O or -O-C 1-2 alkyl-;
  • X 2 represents a direct bond, C 1-2 alkyl, -CO-C,. 2 alkyl or NR 12 -C 1-2 alkyl;
  • R 1 represents hydrogen or halo
  • R 2 represents halo, acetylene or Het 1
  • R 3 represents hydrogen or cyano
  • R 4 represents Ar 4 -C 1-4 alkyloxy-, C 1-4 alkyloxy- or
  • R 12 represents hydrogen or Ci -4 alkyl-
  • R 13 represents Ar 6 -sulfonyl or
  • R 16 and R 17 represents hydrogen, C 1-4 alkyl or R 16 and R 17 taken together with the carbon atom to which they are attached from a C 3-6 cycloalkyl
  • R 23 represents C 1-4 alkyl and R 23 represents hydrogen when R 16 and R 17 taken together with the carbon atom to which they are attached from a C 3-6 cycloalkyl;
  • R 25 , R 26 , R 27 and R 28 each independently represent hydrogen or C ]-4 alkylcarbonyl;
  • Het 1 represents 2-bora-l,3-dioxolanyl;
  • Het 2 represents piperidinyl, piperazinyl, morpholinyl, thiomorpholinyl or 1,1-dioxothiomorpholinyl wherein said Het 2 is optionally substituted with C 1-4 alkyloxycarbonyl or NR 27 R 28 -C 1-4 alkyl;
  • Ar 4 and Ar 5 represents phenyl;
  • Ar 6 represents phenyl optionally substituted with nitro.
  • the X 2 substituent is at position 2', the R 1 substituent represents hydrogen or halo and is at position 4', the R 2 substituent represents halo and is at position 5', the R substituent is at position 2 and the R 4 substituent at position 7 of the structure of formula (I).
  • the X 2 substituent is at position 3', the R 1 substituent represents hydrogen or halo and is at position 4', the R 2 substituent represents halo and is at position 5', the R 3 substituent is at position 2 and the R 4 substituent at position 7 of the structure of formula (I).
  • the compounds of this invention can be prepared by any of several standard synthetic processes commonly used by those skilled in the art of organic chemistry and described for instance in the following references; "Heterocyclic Compounds” - Vol.24 (part4) p 261-304 Fused pyrimidines, Wiley - Interscience ; Chem. Pharm. Bull., VoI 41(2) 362-368 (1993); J.Chem.Soc, Perkin Trans. 1, 2001, 130-137.
  • a particular group of compounds are those compounds of formula (I) were -X 1 - represents -O- hereinafter referred to as the compounds of formula (3).
  • Said compounds are generally prepared starting from the known 6-acetoxy-4-chloro-7-methoxy quinazoline (IF) which can be prepared from commercially available veratric acid and 4-hydroxy-3-methoxy benzoic acid, respectively. Coupling of the latter with suitable substituted anilines (HI') under standard conditions, for example stirred in 2-propanol at an elevated temperature ranging form 40-100 0 C during 3-12 h, furnish the intermediate compounds (IV) (Scheme 1).
  • IF 6-acetoxy-4-chloro-7-methoxy quinazoline
  • HI' suitable substituted anilines
  • V hydrogen or a protective group such as for example, methylcarbonyl, t-butyl, methyl, ethyl, benzyl or trialkylsilyl groups
  • V hydrogen or a protective group such as for example, methylcarbonyl, t-butyl, methyl, ethyl, benzyl or trialkylsilyl groups
  • said macrocyclic compounds of formula (1) are demethylated using art known conditions such as for example provided in Schemes 3&4 hereinbelow, followed by an alkylation with an appropriate alcohol, such as for example described in Scheme 5 hereinafter. Quinazoline demethylation.
  • R represents Ar 4 -Ci. 4 alkyl-, Ci. 4 alkyl- or R represents Ci_ 4 alkyl substituted with one or where possible two or more substituents selected from hydroxy, halo, C M alkyloxy-, NR 7 R 8 or Het 2 -.
  • Ar 4 , Her 2 , R 7 and R 8 are defined as for the compounds of formula (I) hereinbefore.
  • a particular group of compounds are those compounds of formula (3) wherein R represents Ci_ 4 alkyl substituted with NR 7 R 8 or Het 2 wherein said Het 2 is attached to the remainder of the molecule through the nitrogen atom.
  • Said compounds of general formula (7) are generally made according to synthesis scheme 7 departing from the intermediate compounds of general formula (2).
  • R 7 and R 8 are defined as for the compounds of formula (I), or R 7 and R 8 taken together with the nitrogen atom to which they are attached from a heterocycle wherein said heterocycle is defined as Het 2 for the compounds of formula (I) hereinbefore.
  • R 7 and R 8 are defined as for the compounds of formula (I), or R 7 and R 8 taken together with the nitrogen atom to which they are attached from a heterocycle wherein said heterocycle is defined as Het 2 for the compounds of formula (I) hereinbefore.
  • Functional groups which are desirable to protect, include hydroxy, amino and carboxylic acid.
  • Suitable protecting groups for hydroxy include trialkylsilyl groups
  • Suitable protecting groups for amino include tert-butyloxycarbonyl or benzyloxycarbonyl.
  • Suitable protecting groups for carboxylic acid include C ( i -6) alkyl or benzyl esters.
  • the protection and deprotection of functional groups may take place before or after a reaction step.
  • ⁇ -atoms in compounds of formula (I) can be methylated by art- known methods using CH 3 -I in a suitable solvent such as, for example 2-propanone, tetrahydrofuran or dimethylformamide.
  • a suitable solvent such as, for example 2-propanone, tetrahydrofuran or dimethylformamide.
  • the compounds of formula (I) can also be converted into each other following art- known procedures of functional group transformation of which some examples are mentioned hereinafter.
  • the compounds of formula (I) may also be converted to the corresponding TV-oxide forms following art-known procedures for converting a trivalent nitrogen into its TV-oxide form.
  • Said TV-oxidation reaction may generally be carried out by reacting the starting material of formula (I) with 3-phenyl-2-(phenylsulfonyl)oxaziridine or with an appropriate organic or inorganic peroxide.
  • Appropriate inorganic peroxides comprise, for example, hydrogen peroxide, alkali metal or earth alkaline metal peroxides, e.g.
  • organic peroxides may comprise peroxy acids such as, for example, benzenecarboperoxoic acid or halo substituted benzenecarboperoxoic acid, e.g. 3-chlorobenzenecarboperoxoic acid, peroxoalkanoic acids, e.g. peroxoacetic acid, alkylhydroperoxides, e.g. t-butyl hydroperoxide.
  • Suitable solvents are, for example, water, lower alkanols, e.g. ethanol and the like, hydrocarbons, e.g. toluene, ketones, e.g. 2-butanone, halogenated hydrocarbons, e.g. dichloromethane, and mixtures of such solvents.
  • Diastereomers may be separated by physical methods such as fractional crystallization and chromatographic techniques, e.g. counter-current distribution, liquid chromatography and the like.
  • Some of the compounds of formula (I) and some of the intermediates in the present invention may contain an asymmetric carbon atom.
  • Pure stereochemically isomeric forms of said compounds and said intermediates can be obtained by the application of art-known procedures.
  • diastereoisomers can be separated by physical methods such as fractional crystallization or chromatographic techniques, e.g. counter current distribution, liquid chromatography and the like methods.
  • Enantiomers can be obtained from racemic mixtures by first converting said racemic mixtures with suitable resolving agents such as, for example, chiral acids, to mixtures of diastereomeric salts or compounds; then physically separating said mixtures of diastereomeric salts or compounds by, for example, fractional rystallization or chromatographic techniques, e.g. liquid chromatography and the like methods; and finally converting said separated diastereomeric salts or compounds into the corresponding enantiomers.
  • suitable resolving agents such as, for example, chiral acids
  • the growth inhibitory effect and anti- tumour activity of the present compounds has been demonstrated in vitro, in enzymatic assays on the receptor tyrosine kinases such as for example EGFR, AbI, Fyn, FlTl, HcK or the Sar kinase family such as for example Lyn, Yes and cSRC.
  • the growth inhibitory effect of the compounds was tested on a number of carcinamo cell lines, in particular in the ovarian carcinoma cell line SKO V3 and the squamous carcinoma cell line A431 using art known cytotoxicity assays such as MTT.
  • the present invention provides the compounds of formula (I) and their pharmaceutically acceptable JV-oxides, addition salts, quaternary amines and stereochemically isomeric forms for use in therapy. More particular in the treatment or prevention of cell proliferation mediated diseases.
  • the compounds of formula (I) and their pharmaceutically acceptable iV-oxides, addition salts, quaternary amines and the stereochemically isomeric forms may hereinafter be referred to as compounds according to the invention.
  • disorders for which the compounds according to the invention are particularly useful are atherosclerosis, restenosis, cancer and diabetic complications e.g. retinopathy.
  • Said method comprising the systemic or topical administration of an effective amount of a compound according to the invention, to animals, including humans.
  • a therapeutically effective amount of the EGFR inhibitors of the present invention is the amount sufficient to induce the growth inhibitory effect and that this amount varies inter alia, depending on the size, the type of the neoplasia, the concentration of the compound in the therapeutic formulation, and the condition of the patient.
  • an amount of EGFR inhibitor to be administered as a therapeutic agent for treating cell proliferative disorder such as atherosclerosis, restenosis and cancer will be determined on a case by case by an attending physician.
  • a suitable dose is one that results in a concentration of the EGFR inhibitor at the treatment site in the range of 0.5 nM to 200 ⁇ M, and more usually 5 nM to 10 ⁇ M.
  • a patient in need of treatment likely will be administered between 0.01 mg/kg to 300 mg/kg body weight, in particular from 10 mg/kg to 100 mg/kg body weight.
  • the above amounts may vary on a case-by-case basis.
  • the compounds according to the invention are preferably formulated prior to admission.
  • suitable pharmaceutical formulations are prepared by known procedures using well known and readily available ingredients.
  • the compounds of formula (I) as defined above are also useful to mark or identify the kinase domain within the receptor tyrosine kinase receptors.
  • the compounds of the present invention can be labelled, in particular by replacing, partially or completely, one or more atoms in the molecule by their radioactive isotopes.
  • Examples of interesting labelled compounds are those compounds having at least one halo which is a radioactive isotope of iodine, bromine or fluorine; or those compounds having at least one 1 lC-atom or tritium atom.
  • R* is a radioactive halogen atom.
  • any compound of formula (I) containing a halogen atom is prone for radiolabelling by replacing the halogen atom by a suitable isotope.
  • Suitable halogen radioisotopes to this purpose are radioactive iodides, e.g. 122 I, 123 I, 125 I, 131 I; radioactive bromides, e.g. 75 Br, 76 Br, 77 Br and 82 Br, and radioactive fluorides, e.g. 18 F.
  • radioactive halogen atom can be performed by a suitable exchange reaction or by using any one of the procedures as described hereinabove to prepare halogen derivatives of formula (I).
  • Another interesting form of radiolabelling is by substituting a carbon atom by a ⁇ C-atom or the substitution of a hydrogen atom by a tritium atom.
  • said radiolabeled compounds of formula (I) can be used in a process of specifically marking receptor sites in biological material. Said process comprises the steps of (a) radiolabelling a compound of formula (I), (b) administering this radiolabeled compound to biological material and subsequently (c) detecting the emissions from the radiolabeled compound.
  • the compounds are labeled with stable isotopes.
  • the naturally abundant isotopes of hydrogen, carbon and nitrogen 1 H, 12 C and 14 N
  • stable isotopes of these elements 2 H [deuterium], 13 C and 15 N, respectively. Labeling with stable isotopes is used for two principal purposes:
  • the term biological material is meant to comprise every kind of material which has a biological origin. More in particular this term refers to tissue samples, plasma or body fluids but also to animals, specially warm-blooded animals, or parts of animals such as organs.
  • the radiolabeled compounds are administered in an appropriate composition to an animal and the location of said radiolabeled compounds is detected using imaging techniques, such as, for instance, Single Photon Emission Computerized Tomography (SPECT) or Positron Emission Tomography (PET) and the like.
  • imaging techniques such as, for instance, Single Photon Emission Computerized Tomography (SPECT) or Positron Emission Tomography (PET) and the like.
  • SPECT Single Photon Emission Computerized Tomography
  • PET Positron Emission Tomography
  • the present invention provides the use of the compounds according to the invention in the manufacture of a medicament for treating any of the aforementioned cell proliferative disorders or indications.
  • the amount of a compound according to the present invention, also referred to here as the active ingredient, which is required to achieve a therapeutical effect will, of course, vary with the particular compound, the route of administration, the age and condition of the recipient, and the particular disorder or disease being treated.
  • a suitable daily dose would be from 0.01 mg/kg to 300 mg/kg body weight, in particular from 10 mg/kg to 100 mg/kg body weight.
  • a method of treatment may also include administering the active ingredient on a regimen of between one and four intakes per day.
  • the present invention further provides a pharmaceutical composition comprising a compound according to the present invention, together with a pharmaceutically acceptable carrier or diluent.
  • a pharmaceutically acceptable carrier or diluent must be "acceptable" in the sense of being compatible with the other ingredients of the composition and not deleterious to the recipients thereof.
  • compositions of this invention may be prepared by any methods well known in the art of pharmacy, for example, using methods such as those described in Gennaro et al. Remington's Pharmaceutical Sciences (18 th ed., Mack Publishing Company, 1990, see especially Part 8 : Pharmaceutical preparations and their
  • a therapeutically effective amount of the particular compound, in base form or addition salt form, as the active ingredient is combined in intimate admixture with a pharmaceutically acceptable carrier, which may take a wide variety of forms depending on the form of preparation desired for administration.
  • a pharmaceutically acceptable carrier which may take a wide variety of forms depending on the form of preparation desired for administration.
  • These pharmaceutical compositions are desirably in unitary dosage form suitable, preferably, for systemic administration such as oral, percutaneous or parenteral administration; or topical administration such as via inhalation, a nose spray, eye drops or via a cream, gel, shampoo or the like.
  • any of the usual pharmaceutical media may be employed, such as, for example, water, glycols, oils, alcohols and the like in the case of oral liquid preparations such as suspensions, syrups, elixirs and solutions: or solid carriers such as starches, sugars, kaolin, lubricants, binders, disintegrating agents and the like in the case of powders, pills, capsules and tablets.
  • solid carriers such as starches, sugars, kaolin, lubricants, binders, disintegrating agents and the like in the case of powders, pills, capsules and tablets.
  • the carrier will usually comprise sterile water, at least in large part, though other ingredients, for example, to aid solubility, may be included.
  • Injectable solutions may be prepared in which the carrier comprises saline solution, glucose solution or a mixture of saline and glucose solution. Injectable suspensions may also be prepared in which case appropriate liquid carriers, suspending agents and the like may be employed.
  • the carrier optionally comprises a penetration enhancing agent and/or a suitable wetting agent, optionally combined with suitable additives of any nature in minor proportions, which additives do not cause any significant deleterious effects on the skin. Said additives may facilitate the administration to the skin and/or may be helpful for preparing the desired compositions.
  • These compositions may be administered in various ways, e.g., as a transdermal patch, as a spot-on or as an ointment.
  • Dosage unit form as used in the specification and claims herein refers to physically discrete units suitable as unitary dosages, each unit containing a predetermined quantity of active ingredient calculated to produce the desired therapeutic effect in association with the required pharmaceutical carrier.
  • dosage unit forms are tablets (including scored or coated tablets), capsules, pills, powder packets, wafers, injectable solutions or suspensions, teaspoonfuls, tablespoonfuls and the like, and segregated multiples thereof.
  • 'THF' means tetrahydrofuran
  • 'DIPE' means diisopropyl ether
  • 'DMF' means N,N-dimethylformamide
  • 'NaBH(OAc) 3 ' means sodium triacetoxyborohydride
  • 'EtOAc' means ethyl acetate
  • 'EDCF means JV- (ethylcarbonimidoyl)-N,N-dimethyl- 1 ,3-propanediamine monohydrochloride
  • 'HOBT' means 1 -hydroxy- lH-benzotriazole
  • 'CDF means l,l'-carbonylbis-lH-imidazole
  • 'DIPEA' means 7V-ethyl-N-(l -methyl ethyl)- 2-propanamine
  • 'NaBH 4 ' means sodium tetrahydroborate(-l)
  • 'DMA' means dimethylacetamide
  • the desired fractions were purified again by column chromatography over silica gel (eluent: CH 2 C1 2 /(CH 3 OH/NH 3 ) 100/0 to 97/3).
  • the product fractions were collected and the solvent was evaporated.
  • the residue was purified by high-performance liquid chromatography (ammonium acetate buffer).
  • the product was extracted with CH 2 Cl 2 .
  • the separated organic layer was dried and the solvent was evaporated, yielding 0.419 g of compound (7) (S -configuration) .
  • Lithium hydroxide (0.340 g, 0.0081 mol) was added to a mixture of intermediate (44) (0.0006 mol) in CH 3 OH (25ml) and H 2 O (5ml), stirred at room temperature. The reaction mixture was stirred for one hour at 4O 0 C. The mixture was concentrated under reduced pressure to one fifth of the initial volume. The concentrate was poured into water. The mixture was stirred for 30 minutes at room temperature. The precipitate was filtered off, stirred in THF (20ml) for one hour, then the precipitate was filtered off again. The solid was dissolved in THF/CH 3 OH 1/1 (200ml). The whole was filtered and the filtrate was evaporated under reduced pressure. The residue was dried, then stirred for one hour in CH 3 CN. The precipitate was filtered off and dried, yielding 0.142g (48%) of compound (9).
  • the HPLC gradient was supplied by a Waters Alliance HT 2790 system with a column heater set at 4O 0 C. Flow from the column was split to a Waters 996 photodiode array (PDA) detector and a Waters-Micromass ZQ mass spectrometer with an electrospray ionization source operated in positive and negative ionization mode.
  • PDA photodiode array
  • Reversed phase HPLC was carried out on a Xterra MS C18 column (3.5 mm, 4.6 x 100 mm) with a flow rate of 1.6 ml/min.
  • Three mobile phases (mobile phase A 95% 25mM ammonium acetate + 5% acetonitrile; mobile phase B: acetonitrile; mobile phase C: methanol) were employed to run a gradient condition from 100 % A to 50% B and 50% C in 6.5 minutes, to 100 % B in 1 minute, 100% B for 1 minute and reequilibrate with 100 % A for 1.5 minutes.
  • An injection volume of 10 uL was used.
  • Reversed phase HPLC was carried out on a Chromolith (4.6 x 25 mm) with a flow rate of 3 ml/min.
  • Three mobile phases (mobile phase A 95% 25mM ammoniumacetate + 5% acetonitrile; mobile phase B: acetonitrile; mobile phase C: methanol) were employed to run a gradient condition from 96 % A to 2% B and 2% C in 0.9 minutes, to 49 % B and 49 % C in 0.3 minute, 100% B for 0.2 minute.
  • An injection volume of 2 uL was used.
  • Reversed phase HPLC was carried out on a Xterra MS Cl 8 column (3.5 mm, 4.6 x 100 mm) with a flow rate of 1.6 ml/min.
  • Two mobile phases (mobile phase A methanol/H2O; mobile phase B 0.1 % formic acid) were employed to run a gradient condition from 100 % B to 5 % B 12 minutes. An injection volume of 10 uL was used.
  • Reversed phase HPLC was carried out on a Xterra MS C18 column (3.5 mm, 4.6 x 100 mm) with a flow rate of 1.6 ml/min.
  • Three mobile phases (mobile phase A 95% 25mM ammonium acetate + 5% acetonitrile; mobile phase B: acetonitrile; mobile phase C: methanol) were employed to run a gradient condition from 100 % A to 30% A, 35 % B; 35 % C in 3 minutes to 50 % B and 50% C in 3.5 minutes, to 100 % B in 0.5 minute.
  • An injection volume of 10 uL was used.
  • Reversed phase HPLC was carried out on a Kromasil C18 column (3.5 mm, 4.6 x 100 mm) with a flow rate of 1 ml/min.
  • Three mobile phases (mobile phase A ammonium acetate; mobile phase B: acetonitrile; mobile phase C: formic acid) were employed to run a gradient condition from 30 % A, 40 % B, 30 % C for 1 minute to 100 % B for 5 minutes. An injection volume of 10 uL was used.
  • the activity of the kinase of interest is measured using an appropriate substrate that is incubated with the aforementioned kinase protein in the presence of ( 33 P) radiolabeled ATP.
  • 33 P Phosporylation of the substrate is subsequently measured as radioactivity bound on a glassfiber-filter.
  • kinases are pre-diluted to a 10x working concentration prior to addition into the assay.
  • the composition of the dilution buffer for each kinase is detailed below.
  • AbI human In a final reaction volume of 25 ⁇ l, AbI (h) (5-10 mU) is incubated with 8 mM MOPS pH 7.0, 0.2 mM EDTA, 50 ⁇ M EAIYAAPFAKKK, 10 mM MgAcetate and [ ⁇ - 33 P- ATP] (specific activity approx. 500 cpm/pmol, concentration as required). The reaction is initiated by the addition of the MgATP mix. After incubation for 40 minutes at room temperature, the reaction is stopped by the addition of 5 ⁇ l of a 3% phosphoric acid solution. 10 ⁇ l of the reaction is then spotted onto a P30 filtermat and washed three times for 5 minutes in 75 mM phosphoric acid and once in methanol prior to drying and scintillation counting.
  • BIk (m) (5-10 mU) is incubated with 50 mM Tris pH 7.5, 0.1 mM EGTA, 0.1 mM Na3VO4, 0.1% ⁇ -mercaptoethanol, 0.1 mg/ml poly(Glu, Tyr) 4:1, 10 mM MgAcetate and [ ⁇ -33P-ATP] (specific activity approx. 500 cpm/pmol, concentration as required).
  • the reaction is initiated by the addition of the MgATP mix. After incubation for 40 minutes at room temperature, the reaction is stopped by the addition of 5 ⁇ l of a 3% phosphoric acid solution. 10 ⁇ l of the reaction is then spotted onto a Filtermat A and washed three times for 5 minutes in 75 mM phosphoric acid and once in methanol prior to drying and scintillation counting.
  • Bmx (h) (5-10 mU) is incubated with 8 mM MOPS pH 7.0, 0.2 mM EDTA, 0.1 mg/ml poly(Glu, Tyr) 4:1, 10 mM MgAcetate and [ ⁇ -33P- ATP] (specific activity approx. 500 cpm/pmol, concentration as required).
  • the reaction is initiated by the addition of the MgATP mix. After incubation for 40 minutes at room temperature, the reaction is stopped by the addition of 5 ⁇ l of a 3% phosphoric acid solution. 10 ⁇ l of the reaction is then spotted onto a Filtermat A and washed three times for 5 minutes in 75 mM phosphoric acid and once in methanol prior to drying and scintillation counting.
  • CDK5/p35 human In a final reaction volume of 25 ⁇ l, CDK5/p35 human (5-10 mU) is incubated with 8 mM MOPS pH 7.0, 0.2 mM EDTA, 0.1 mg/ml histone Hl, 10 mM MgAcetate and [ ⁇ - 33 P-ATP] (specific activity approx. 500cpm/pmol, concentration as required).
  • the reaction is initiated by the addition of the MgATP mix. After incubation for 40 minutes at room temperature, the reaction is stopped by the addition of 5 ⁇ l of a 3% phosphoric acid solution. 10 ⁇ l of the reaction is then spotted onto a P30 filtermat and washed three times for 5 minutes in 75 mM phosphoric acid and once in methanol prior to drying and scintillation counting.
  • CDK6/cvclinD3 human In a final reaction volume of 25 ⁇ l, CDK6/cyclinD3 human (5-10 mU) is incubated with 8 mM MOPS pH 7.0, 0.2 mM EDTA, 0.1 mg/ml histone Hl, 10 mM MgAcetate and [ ⁇ - 33 P-ATP] (specific activity approx. 500 cpm/pmol, concentration as required). The reaction is initiated by the addition of the MgATP mix. After incubation for 40 minutes at room temperature, the reaction is stopped by the addition of 5 ⁇ l of a 3% phosphoric acid solution. 10 ⁇ l of the reaction is then spotted onto a P30 filtermat and washed three times for 5 minutes in 75 mM phosphoric acid and once in methanol prior to drying and scintillation counting.
  • cSRC human In a final reaction volume of 25 ⁇ l, cSRC (h) (5-10 mU) is incubated with 8 mM
  • the reaction is initiated by the addition of the MgATP mix. After incubation for 40 minutes at room temperature, the reaction is stopped by the addition of 5 ⁇ l of a 3% phosphoric acid solution.
  • EGFR (h) (5-10 mU) is incubated with 8 mM MOPS pH 7.0, 0.2 mM EDTA, 1OmM MnC12, 0.1 mg/ml poly(Glu, Tyr) 4:1, 10 mM
  • MgAcetate and [ ⁇ - P-ATP] (specific activity approx. 500 cpm/pmol, concentration as required).
  • the reaction is initiated by the addition of the MgATP mix. After incubation for 40 minutes at room temperature, the reaction is stopped by the addition of 5 ⁇ l of a 3% phosphoric acid solution. 10 ⁇ l of the reaction is then spotted onto a Filtermat A and washed three times for 5 minutes in 75 mM phosphoric acid and once in methanol prior to drying and scintillation counting.
  • ErbB4 (h) (5-10 mU) is incubated with 8 mM MOPS pH 7.0, 0.2 mM EDTA, 10 mM MnC12, 0.1 mg/ml poly(Glu, Tyr) 4:1, 10 mM MgAcetate and [ ⁇ -33P-ATP] (specific activity approx. 500 cpm/pmol, concentration as required).
  • the reaction is initiated by the addition of the MgATP mix. After incubation for 40 minutes at room temperature, the reaction is stopped by the addition of 5 ⁇ l of a 3% phosphoric acid solution. 10 ⁇ l of the reaction is then spotted onto a Filtermat A and washed three times for 5 minutes in 75 mM phosphoric acid and once in methanol prior to drying and scintillation counting.
  • Fgr human (5-10 mU) is incubated with 8 mM MOPS pH 7.0, 0.2 mM EDTA, 0.1 mg/ml poly(Glu, Tyr) 4:1, 10 mM MgAcetate and [ ⁇ - 33 P-ATP] (specific activity approx. 500 cpm/pmol, concentration as required).
  • the reaction is initiated by the addition of the MgATP mix. After incubation for 40 minutes at room temperature, the reaction is stopped by the addition of 5 ⁇ l of a 3% phosphoric acid solution. 10 ⁇ l of the reaction is then spotted onto a Filtermat A and washed three times for 5 minutes in 75 mM phosphoric acid and once in methanol prior to drying and scintillation counting.
  • Fyn human (5-10 mU) is incubated with 50 mM Tris pH 7.5, 0.1 mM EGTA, 0.1 mM Na 3 VO 4 , 250 ⁇ M KVEKIGEGTYGVVYK (Cdc2 peptide), 10 mM MgAcetate and [ ⁇ - 33 P-ATP] (specific activity approx. 500 cpm/pmol, concentration as required).
  • the reaction is initiated by the addition of the MgATP mix.
  • the reaction is stopped by the addition of 5 ⁇ l of a 3% phosphoric acid solution. 10 ⁇ l of the reaction is then spotted onto a P30 filtermat and washed three times for 5 minutes in 75 mM phosphoric acid and once in methanol prior to drying and scintillation counting.
  • Lck (h) (5-10 mU) is incubated with 50 mM Tris pH 7.5, 0.1 mM EGTA, 0.1 mM Na3VO4, 250 ⁇ M KVEKIGEGTYGVVYK (Cdc2 peptide), 10 mM MgAcetate and [ ⁇ - 33 P-ATP] (specific activity approx. 500 cpm/pmol, concentration as required). The reaction is initiated by the addition of the MgATP mix.
  • Lyn (h) (5-10 mU) is incubated with 50 mM Tris pH 7.5, 0.1 mM EGTA, 0.1 mM Na3VO4, 0.1% ⁇ -mercaptoethanol, 0.1 mg/ml poly(Glu, Tyr) 4:1, 10 mM MgAcetate and [ ⁇ - 33 P-ATP] (specific activity approx. 500 cpm/pmol, concentration as required).
  • the reaction is initiated by the addition of the MgATP mix. After incubation for 40 minutes at room temperature, the reaction is stopped by the addition of 5 ⁇ l of a 3% phosphoric acid solution. 10 ⁇ l of the reaction is then spotted onto a Filtermat A and washed three times for 5 minutes in 75 mM phosphoric acid and once in methanol prior to drying and scintillation counting.
  • Ret human (5-10 mU) is incubated with 8 mM MOPS pH 7.0, 0.2 mM EDTA, 250 ⁇ M KKKSPGEYVNIEFG, 10 mM MgAcetate and [ ⁇ - 33 P-ATP] (specific activity approx. 500 cpm/pmol, concentration as required).
  • the reaction is initiated by the addition of the MgATP mix. After incubation for 40 minutes at room temperature, the reaction is stopped by the addition of 5 ⁇ l of a 3% phosphoric acid solution. 10 ⁇ l of the reaction is then spotted onto a P30 filtermat and washed three times for 5 minutes in 75 mM phosphoric acid and once in methanol prior to drying and scintillation counting.
  • Yes (h) (5-10 mU) is incubated with 8 mM MOPS pH 7.0, 0.2 mM EDTA, 0.1 mg/ml poly(Glu, Tyr) 4:1, 10 mM MgAcetate and [ ⁇ - 33 P- ATP] (specific activity approx. 500 cpm/pmol, concentration as required).
  • the reaction is initiated by the addition of the MgATP mix. After incubation for 40 minutes at room temperature, the reaction is stopped by the addition of 5 ⁇ l of a 3% phosphoric acid solution. 10 ⁇ l of the reaction is then spotted onto a Filtermat A and washed three times for 5 minutes in 75 mM phosphoric acid and once in methanol prior to drying and scintillation counting.
  • Fltl human (5-10 mU) is incubated with 8 mM MOPS pH 7.0, 0.2 mM EDTA, 250 ⁇ M KKKSPGEYVNIEFG, 10 mM MgAcetate and [ ⁇ - 33 P-ATP] (specific activity approx. 500 cpm/pmol, concentration as required).
  • the reaction is initiated by the addition of the MgATP mix. After incubation for 40 minutes at room temperature, the reaction is stopped by the addition of 5 ⁇ l of a 3% phosphoric acid solution. 10 ⁇ l of the reaction is then spotted onto a P30 filtermat and washed three times for 5 minutes in 75 mM phospho ⁇ c acid and once in methanol p ⁇ or to drying and scintillation counting.
  • Hck human (5-10 mU) is incubated with 8 mM MOPS pH 7.0, 0.2 mM EDTA, 250 ⁇ M KVEKIGEGTYGVVYK (Cdc2 peptide), 10 mM MgAcetate and [ ⁇ - 33 P-ATP] (specific activity approx.
  • the reaction is initiated by the addition of the MgATP mix After incubation for 40 minutes at room temperature, the reaction is stopped by the addition of 5 ⁇ l of a 3% phospho ⁇ c acid solution 10 ⁇ l of the reaction is then spotted onto a P30 filtermat and washed three times for 5 minutes in 75 mM phospho ⁇ c acid and once in methanol p ⁇ or to drying and scintillation counting.
  • Score 1 10-30% inhibition
  • Score 2 30-60% inhibition
  • Score 3 60-80% inhibition
  • Score 4 > 80% inhibition.
  • Active ingredient as used throughout these examples relates to a compound of formula (I) or a pharmaceutically acceptable addition salt thereof.
  • Example D.I film-coated tablets Preparati . on . of tablet core
  • a mixture of A.I. (100 g), lactose (570 g) and starch (200 g) was mixed well and thereafter humidified with a solution of sodium dodecyl sulfate (5 g) and polyvinylpyrrolidone (10 g) in about 200ml of water.
  • the wet powder mixture was sieved, dried and sieved again.
  • microcrystalline cellulose (100 g) and hydrogenated vegetable oil (15 g) The whole was mixed well and compressed into tablets, giving 10.000 tablets, each comprising 10 mg of the active ingredient.

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Abstract

The present invention concerns kinase inhibitors formula, formula (I), the N-oxide forms, the pharmaceutically isomeric forms thereof, wherein Z represents NH and the other substituents are defined as in the claims.

Description

MTKI QUINAZOLINE DERIVATIVES
The human genome encompasses some 2,000 proteins that utilize adenosine 5'- triphosphate (ATP) in one way or another and some 500 of these encode for protein kinases, i.e the protein-tyrosine and protein-serine/threonine kinases, that share a catalytic domain conserved in sequence and structure but which are notably different in how their catalysis is regulated. Substrate phosphorylation by these enzymes is nature's predominant molecular way of organizing cellular signal transduction and regulating biochemical processes in general. It is not surprising, therefore, that abnormal phosphorylation of cellular proteins is a hallmark of disease and that there is a growing interest in the use of kinase inhibitors as drugs for therapeutic intervention in many disease states such as cancer, diabetes, inflammation and arthritis.
It is an object of the present invention to provide such kinase inhibitors, that are quinazoline derived macrocycles, hereinafter also referred to as multi targeting kinase inhibitors (MTKI), found to possess anti-proliferative activity, such as anti-cancer activity and which are accordingly useful in methods of treatment of the human or animal body, for example in the manufacture of medicaments for use in hyper proliferative disorders such as atherosclerosis, restenosis and cancer. The invention also relates to processes for the manufacture of said quinazoline derivatives, to pharmaceutical compositions containing them and to their use in the manufacture of medicaments of use in the production of anti-proliferative effect .
In particular, the compounds of the present invention were found to inhibit tyrosine kinase enzymes, also called tyrosine kinases. Tyrosine kinases are a class of enzymes, which catalyse the transfer of the terminal phosphate of adenosine triphosphate to the phenolic hydroxy- group of a tyrosine residue present in the target protein. It is known, that several oncogenes, involved in the transformation of a cell into a malignant tumour cell, encode tyrosine kinase enzymes including certain growth factor receptors such as EGF, FGF, IGF-IR, IR, PDGF and VEGF. This family of receptor tyrosine kinases and in particular the EGF family of receptor tyrosine kinases are frequently present in common human cancers such as breast cancer, non-small cell lung cancers including adenocarcinomas and squamous cell cancer of the lung, bladder cancer, oesophageal cancer, gastrointestinal cancer such as colon, rectal or stomach cancer, cancer of the prostate, leukaemia and ovarian, bronchial or pancreatic cancer, which are examples of cell proliferation disorders. Accordingly, it has been recognised that the selective inhibition of tyrosine kinases will be of value in the treatment of cell proliferation related disorders. Support for this view is provided by the development of Herceptin® (Trastuzumab) and Gleevec™ (imatinib mesylate) the first examples of target based cancer drugs. Herceptin® (Trastuzumab) is targeted against Heτ2/neu, a receptor tyrosine kinase found to be amplified up to 100-fold in about 30% of patients with invasive breast cancer. In clinical trials Herceptin® (Trastuzumab) proved to have anti-tumour activity against breast cancer (Review by L.K. Shawer et al, "Smart Drugs: Tyrosine kinase inhibitors in cancer therapy", 2002, Cancer Cell Vol.l, 117), and accordingly provided the proof of principle for therapy targeted to receptor tyrosine kinases. The second example, Gleevec™ (imatinib mesylate), is targeted against the abelson tyrosine kinase (BcR-AbI), a constitutively active cytoplasmic tyrosine kinase present in virtually all patients with chronic myelogenous leukaemia (CML) and 15% to 30% of adult patients with acute lymphoblastic leukaemia. In clinical trials Gleevec™ (imatinib mesylate) showed a spectacular efficacy with minimal side effects that led to an approval within 3 months of submission. The speed of passage of this agent through clinical trials and regulatory review has become a case study in rapid drug development (Drucker BJ. & Lydon N., "Lessons learned from the development of an AbI tyrosine kinase inhibitor for chronic myelogenous leukaemia.", 2000, J.Clin. Invest. 105, 3).
Further support is given by the demonstration that EGF receptor tyrosine kinase inhibitors, specifically attenuates the growth in athymic nude mice of transplanted carcinomas such as human mammary carcinoma or human squamous cell carcinoma (Review by T.R. Burke Jr., Drugs of the Future, 1992, 17, 119). As a consequence, to treat different cancers there has been considerable interest in the development of drugs that target the EGFR receptor. For example, several antibodies that bind to the extracellular domain of EGFR are undergoing clinical trials, including Erbitux™ (also called C225, Cetuximab), which was developed by Imclone Systems and is in Phase IU clinical trials for the treatment of several cancers. Also, several promising orally active drugs that are potent and relatively specific inhibitors of the EGFR tyrosine kinase are now well advanced in clinical trials. The AstraZeneca compound ZD 1839, which is now called IRESSA® and approved for the treatment of advanced non-small-cell lung cancer, and the OSI/Genentech/Roche compound OSI-774, which is now called Tarceva™ (erlotinib) , have shown marked efficacy against several cancers in human clinical trials (Morin MJ. , "From oncogene to drug: development of small molecule tyrosine kinase inhibitors as anti-tumour and anti-angiogenic agents, 2000, Oncogene 19, 6574). In addition to the above, EGF receptor tyrosine kinases are shown to be implicated in non-malignant proliferative disorders such as psoriasis (Elder et al., Science, 1989, 243; 811). It is therefore expected that inhibitors of EGF type receptor tyrosine kinases will be useful in the treatment of non-malignant diseases of excessive cellular proliferation such as psoriasis, benign prostatic hypertrophy, atherosclerosis and restenosis.
It is disclosed in International Patent Application WO96/33980 and in J. Med. Chem, 2002, 45, 3865 that certain 4 anilino substituted quinazoline derivatives may be useful as inhibitors of tyrosine kinase and in particular of the EGF type receptor tyrosine kinases. Unexpectedly it was found that Quinazoline derivatives of the present formula (I) that are different in structure show to have tyrosine kinase inhibitory activity.
It is accordingly an object of the present invention to provide further tyrosine kinase inhibitors useful in the manufacture of medicaments in the treatment of cell proliferative related disorders.
This invention concerns compounds of formula (I)
Figure imgf000004_0001
the N-oxide forms, the pharmaceutically acceptable addition salts and the stereochemically isomeric forms thereof, wherein
Z represents NH;
Y represents -C3-9alkyl-, -C2.9alkenyl-, -C1-5alkyl-oxy-C1-5alkyl-,
-Ci.5alkyl-NR13-Ci-5alkyl-, -C1-5alkyl-NR14-CO-C1-5alkyl-, -Ci^alkyl-NH-CO-, -NH-CO-CLealkyl-, -CO-C1-7alkyl-, -C1-7alkyl-CO-, C]-6alkyl-CO-C1-6alkyl,
-C1-2alkyl-NR ,23 -CO-CR , 1160τR-> 17 -NH-,
Figure imgf000004_0002
-C1-2alkyl-CO-NR2°-Ci.3alkyl-CO-, -Cμzalkyl-NR^-CHrCO-NH-Cμsalkyl-, -NR22-CO-Ci-3alkyl-NH-, -C1-3alkyl-NH-CO-Het20-, C1-2alkyl-CO-Het21-CO-, or
-Het22-CH2-CO-NH-Ci-3alkyl-; X1 represents O, -O-C^alkyl-, -0-N=CH-, NR11 or -NRπ-C1-2alkyl-; in a particular embodiment X1 represents O, -O-C1-2alkyl- or NRH-C1-2alkyl; X2 represents a direct bond, C].2alkyl, O, -O-Ci-2alkyl-, CO, -CO-C^alkyl-, -O-N=CH-
, NR12 or NR12-Ci_2alkyl-; in a particular embodiment X2 represents a direct bond,
-0-, -O-C1-2alkyl, -CO-C1-2alkyl- or NR12-Ci_2alkyl-; R1 represents hydrogen, cyano, halo or hydroxy, preferably halo; R2 represents hydrogen, cyano, halo, hydroxy, hydroxycarbonyl-, C1-4alkyloxycarbonyl-, Het16-carbonyl-, Ci_4alkyl-, C2.6alkynyl-, Ar5, Het1 or dihydroxyborane ; R3 represents hydrogen, cyano, halo, hydroxy, formyl, C1-6alkoxy-, Cμδalkyl-,
C1-OaIkOXy- substituted with halo, or R3 represents C1-4alkyl substituted with one or where possible two or more substituents selected from hydroxy or halo; R4 represents Ar4-C1-4alkyloxy-, C1-4alkyloxy- or R4 represents C1-4alkyloxy substituted with one or where possible two or more substituents selected from hydroxy-, halo,
C1-4alkyloxy-, C1-4alkyloxy-C1-4alkyloxy-, NR37R38-carbonyloxy-, Het5- carbonyloxy-, NR7R8, NR9R10-carbonyl-, Het3-carbonyl-, Het13-oxy- or Het2-; R7 represents hydrogen, hydroxy-C1-4alkyl- or C1-4alkyl; R8 represents C3-6cycloalkyl; Het6-carbonyl-; Het7-aminocarbonyl-; Het8;
Het9-oxycarbonyl-; Het10-sulfonyl-; C1-4alkyloxycarbonyl; mono- or di(C1-4alkyl)aminocarbonyl-; mono- or di(C1-4alkyl)aminocarbonyl substituted with d-4alkylsulfonyl-; or
C^alkylcarbonyl optionally substituted with one or more substituents selected from C1-4alkylsulfonyl, hydroxy- and C^alkyloxy-; or
R8 represents Cμ4alkyl substituted with one or more substituents selected from
C1-4alkylsulfonyl-, NR25R26, aminocarbonyloxy-, C1-4alkylcarbonyloxy-, aminocarbonyl-, hydroxy-C^alkyloxy-, C^alkyloxy-Ci^alkyloxy-, and Het11; R9 represents hydrogen or C1-4alkyl-; R10 represents Het4 or C1-4alkyl- substituted with C^alkylsulfonyl-, ; R11 represents hydrogen, C1-4alkyl- or C1-4alkyl-oxy-carbonyl-; R12 represents hydrogen, C1-4alkyl-, C1-6alkyloxycarbonyl- or C1-6alkyloxycarbonyl- substituted with phenyl;
R13 represents hydrogen, Het^C^alkyl, C].6alkyloxycarbonyl optionally substituted with phenyl or R13 represents Ar6-sulfonyl or Het24-C1.4alkylcarbonyl; in particular morpholinyl-C^alkyl; R14 and R15 are each independently selected from hydrogen, d-4alkyl, Het15-d.4alkyl- or d_4a]ky]oxyd.4a]ky]-;
R16 and R17 each independently represents hydrogen, d-4alkyl or d_4alkyl substituted with hydroxy-, C3.6cycloalkyl or phenyl; or R16 and R17 taken together with the carbon atom to which they are attached form a C3-6cycloalkyl;
R18 represents hydrogen or d_4alkyl optionally substituted with hydroxy or phenyl;
R19 represents hydrogen or C1-4alkyl, in particular hydrogen or methyl, even more particular hydrogen;
R20 represents hydrogen or C1-4alkyl, in particular hydrogen or methyl; R21 represents hydrogen, Ci-4alkyl, Het23-C1-4alkylcarbonyl- or
R21 represents mono-or di(Ci-4alkyr)amino-d-4alkyl-carbonyl- optionally substituted with hydroxy, pyrimidinyl, dimethylamine or d_4alkyloxy;
R22 represents hydrogen or Ci.4alkyl optionally substituted with hydroxy or
Ci-4alkyloxy; R23 represents C1-4alkyl optionally substituted with hydroxy-, Ci-4alkyloxy- or Het25; R23 may also represent hydrogen when R16 and R17 taken together with the carbon atom to which they are attached form a C3-6cycloalkyl;
R25 and R26 each independently represent hydrogen, C1-4alkyl, Ci-4alkylsulfonyl-, aminocarbonyl-, mono- or di(d_4alkyl)aminocarbonyl-, C1-4alkylcarbonyl-, C1-4alkyloxycarbonyl- or d.4alkyl substituted with one or more substituents selected from C1-4alkylsulfonyl-, hydroxy- and Ci-4alkyloxy-, in particular R25 and R26 each independently represent hydrogen, C1-4alkyl, C1-4alkylsulfonyl-, aminocarbonyl-, mono- or di(Ci-4alkyl)aminocarbonyl- or d_4alkylcarbonyl-;
R27 and R28 each independently represent hydrogen, Ci-4alkyl, C1-4alkylsulfonyl-, aminocarbonyl-, mono- or diCd^alky^aminocarbonyl-, Ci-4alkylcarbonyl-,
C1-4alkyloxycarbonyl- or C1-4alkyl substituted with one or more substituents selected from C1-4alkylsulfonyl-, hydroxy- and d^alkyloxy-; or for those compounds of formula (I) wherein Het2 represents a heterocycle selected from morpholinyl, piperazinyl, piperidinylypyrrolidinyl or thiomorpholinyl substituted with NR27R28-C1-4alkyl said R27 and R28 each independently represent d_4alkylsulfonyl-, aminocarbonyl-, mono- or di(d_4alkyl)aminocarbonyl-, Ci-4alkylcarbonyl-,
Figure imgf000006_0001
substituted with one or more substituents selected from C1-4alkylsulfonyl-, hydroxy- and Ci_4alkyloxy-;
R29 and R30 each independently represent hydrogen, aminosulfonyl, aminocarbonyl, mono- or di(C1-4alkyl)aminocarbonyl-, mono- or di(Ci.4alkyl)aminosulfonyl-, or
Ci_4alkyl- optionally substituted with one or more substituents selected from NR31R32, C1-4alkylsulfonyl, aminocarbonyloxy-, hydroxy-, d-4alkyloxy-, aminocarbonyl- and mono- or di(Ci-4alkyl)aminocarbonyl-, or
C].4alkyloxycarbonyl optionally substituted with one or more substituents selected from hydroxy, C1-4alkyloxy- and C1-4alkylsulfonyl-, or
C1-4alkylcarbonyl optionally substituted with one or more substituents selected from hydroxy-, C1-4alkyloxy- and C1_4alkylsulfonyl-;
R31 and R32 each independently represent hydrogen, C1-4alkyl, C1-4alkylsulfonyl-, aminocarbonyl-, mono- or di(C1-4alkyl)aminocarbonyl-, C1-4alkylcarbonyl-,
Ci^alkyloxycarbonyl- or C1-4alkyl substituted with one or more substituents selected from Q^alkylsulfonyl-, hydroxy- and C1-4alkyloxy-; R33 represents hydrogen or C1-4alkyl;
R34 represents Ci_4alkylsulfonyl-, aminocarbonyl-, mono- or di(C1-4alkyl)aminocarbonyl-, C1.4alkylcarbonyl-, C1-4alkyloxycarbonyl- or
C]-4alkyl substituted with one or more substituents selected from Ci-4alkylsulfonyl-
, hydroxy- and C1-4alkyloxy-; R35 represents hydrogen or C1-4alkyl;
R36 represents C^alkylsulfonyl-, aminocarbonyl-, mono- or di(C1-4alkyl)aminocarbonyl-, C^alkylcarbonyl-, C1-4alkyloxycarbonyl- or
C1-4alkyl substituted with one or more substituents selected from Ci-4alkylsulfonyl-
, hydroxy- and Ci_4alkyloxy-; R37 and R38 each independently represent hydrogen, Ci^alkyl, C]-4alkylsulfonyl-, Het12 or C1-4alkyl substituted with one or more substituents selected from
C1_4alkylsulfonyl-, hydroxy- and C1-4alkyloxy-; R39 and R40 each independently represent aminosulfonyl, aminocarbonyl, mono- or di(C1-4alkyl)aminocarbonyl-, mono- or di(C]-4alkyl)aminosulfonyl-, or C1-4alkyl- substituted with one or more substituents selected from NR31R32,
Ci-4alkylsulfonyl, aminocarbonyloxy-, hydroxy-, C1-4alkyloxy-, aminocarbonyl- and mono- or di(C1-4alkyl)aminocarbonyl-, or
C1-4alkyloxycarbonyl optionally substituted with one or more substituents selected from hydroxy-, C].4alkyloxy- and C1-4alkylsulfonyl-, or C1-4alkylcarbonyl optionally substituted with one or more substituents selected from hydroxy-, Q^alkyloxy- and C1-4alkylsulfonyl-; Het1 represents thiazolyl or 2-bora-l,3-dioxolanyl wherein said Het1 is optionally substituted with one or where possible two, three, four or more substituents selected from amino, C1-4alkyl, hydroxy-C1-4alkyl-, phenyl, phenyl-C].4alkyl-, Ci^alkyl-oxy-Ci^alkyl-, mono- or di(C].4alkyl)amino- or amino-carbonyl-;
Het2 represents a heterocycle selected from tetrahydropyranyl, tetrahydrofuranyl, furanyl, 1,1-dioxothiomorpholinyl, piperazininonyl, tetrahydro-l,l-dioxido-2H- thiopyranyl, piperidinonyl, azetidinyl or 2-azetidinonyl wherein said Het2 is optionally substituted with one or where possible two or more substituents selected from hydroxy, amino, NR29R30, aminocarbonyl, mono- or di(C1-4alkyl)aminocarbonyl, C1-4alkylsulfonyl or Ci_4alkyl- optionally substituted with one or more substituents selected from
NR27R28, Q^alkylsulfonyl, aminocarbonyloxy-, aminocarbonyl- and mono- or di(C1-4alkyl)aminocarbonyl-, or
C1-4alkyloxy- optionally substituted with C1-4alkyloxy-, or
C1-4alkyloxycarbonyl optionally substituted with one or more substituents selected from hydroxy, C1-4alkyloxy- and Ci-4alkylsulfonyl-, or
C1-4alkylcarbonyl optionally substituted with one or more substituents selected from hydroxy-, C^alkyloxy- and C1-4alkylsulfonyl-; or Het2 represents a heterocycle selected from morpholinyl, piperazinyl, piperidinyl, pyrrolidinyl, thiomorpholinyl or 1,1-dioxothiomorpholinyl wherein said Het2 is optionally substituted with one or where possible two or more substituents selected from
C1-4alkyl- optionally substituted with one or more substituents selected from
NR27R28, C1-4alkylsulfonyl, aminocarbonyloxy-, aminocarbonyl- and mono- or dKC^alkyOaminocarbonyl-, or Ci-4alkyloxy- optionally substituted with Ci-4alkyloxy-, or
C^alkyloxycarbonyl optionally substituted with one or more substituents selected from hydroxy, C1-4alkyloxy- and C1-4alkylsulfonyl-, or
C1-4alkylcarbonyl optionally substituted with one or more substituents selected from hydroxy-, Ci-4alkyloxy- and C1-4alkylsulfonyl-; Het3 represents a heterocycle selected from tetrahydropyranyl, tetrahydrofuranyl, furanyl, 1,1-dioxothiomorpholinyl, piperazininonyl, tetrahydro-l,l-dioxido-2H- thiopyranyl, piperidinonyl, azetidinyl or 2-azetidinonyl wherein said Het3 is optionally substituted with one or where possible two or more substituents hydroxy-, amino, C].4alkyl-, C3-6cycloalkyl-C]-4alkyl-, aminosulfonyl-, mono- or di(C1-4alkyl)aminosulfonyl-, amino-C].4alkyl-, Mono- or ditd^alkyOamino-
Ci-4alkyl, NR35R36, C1-4alkyl-sulfonyl-C1-4alkyl- or C]_4alkyloxy- optionally substituted with C1-4alkyloxy- or hydroxy; or Het3 represents a heterocycle selected from morpholinyl, piperazinyl, piperidinyl, furanyl or pyrrolidinyl wherein said Het3 is substituted with one or where possible two or more substituents selected from NR35R36, Ci-4alkyl-sulfonyl-C1-4alkyl- or
C]_4alkyloxy- optionally substituted with Ci-4alkyloxy- or hydroxy; Het4 represents a heterocycle selected from morpholinyl, piperazinyl, piperidinyl, furanyl, pyrazolyl, dioxolanyl, thiazolyl, oxazolyl, imidazolyl, isoxazolyl, oxadiazolyl, pyridinyl or pyrrolidinyl wherein said Het4 is substituted with one or where possible two or more substituents selected from Ci_4alkyl-sulfonyl- C1-4alkyl-, C^alkyloxy- optionally substituted with Ci-4alkyloxy- or hydroxy;
Het5 represents a heterocycle selected from furanyl, piperazinyl,
1,1-dioxothiomorpholinyl, piperazininonyl, piperidinyl, tetrahydro-l,l-dioxido- 2H-thiopyranyl, piperidinonyl, morpholinyl or pyrrolidinyl wherein said Het5 is optionally substituted with hydroxy, amino, mono- or di(C1-4alkyl)-amino-, C1-4alkyl,
Het6 and Het7 each independently represents a heterocycle selected from piperazinyl, piperidinyl or pyrrolidinyl wherein said heterocycles are optionally substituted with one or more substituents selected from hydroxy-, amino, hydroxy-Ci-4alkyl-, C1-4alkyloxy-C1-4alkyl- and C^alkyl-; Het8 represents a heterocycle selected from tetrahydropyranyl, tetrahydrofuranyl, 1 , 1 -dioxothiomorpholinyl, piperazininonyl, tetrahydro- 1 , l-dioxido-2H- thiopyranyl, piperidinonyl, azetidinyl or 2-azetidinonyl wherein said Het8 is optionally substituted with aminosulfonyl, aminocarbonyl, mono- or d^Q^alkytyaminocarbonyl-, mono- or di(C1-4alkyl)aminosulfonyl-, or C1-4alkyl- optionally substituted with one or more substituents selected from amino, mono- or di(C1-4alkyl)amino-, NR33R34, C^alkylsulfonyl, aminocarbonyloxy-, hydroxy-, C1-4alkyloxy-, aminocarbonyl- and mono- or di(C1-4alkyl)aminocarbonyl-, or C^alkyloxycarbonyl optionally substituted with one or more substituents selected from hydroxy, C1-4alkyloxy- and Ci_4alkylsulfonyl-, or
Ci-4alkylcarbonyl optionally substituted with one or more substituents selected from hydroxy, C1-4alkyloxy- and Ci_4alkylsulfonyl-; or
Het8 represents a heterocycle selected from furanyl, piperidinyl or piperazinyl wherein said Het8 is substituted with aminocarbonyl, mono- or di(C1-4alkyl)aminocarbonyl-, mono- or di(C1-4alkyl)aminosulfonyl-, or
C1-4alkyl- substituted with one or more substituents selected from NR33R34, Ci_4alkylsulfonyl, aminocarbonyloxy-, hydroxy-, C1-4alkyloxy-, aminocarbonyl- and mono- or di(Ci-4alkyl)aminocarbonyl-, or Ci-4alkyloxycarbonyl optionally substituted with one or more substituents selected from hydroxy, Ci-4alkyloxy- and Ci^alkylsulfonyl-, or
C].4alkylcarbonyl optionally substituted with one or more substituents selected from hydroxy, Ci-4alkyloxy- and Ci_4alkylsulfonyl-; Het9 and Het10 each independently represents a heterocycle selected from piperazinyl, piperidinyl or pyrrolidinyl wherein said heterocycles are optionally substituted with one or more substituents selected from hydroxy-, amino, hydroxy-Ci-4alkyl-, C1-4alkyloxy-C1-4alkyl- and C^alkyl-;
Het11 represents 2-imidazolidinonyl- or ^ ° ;
Het12 represents a heterocycle selected from morpholinyl, piperazinyl, piperidinyl or pyrrolidinyl wherein said Het12 is optionally substituted with one or where possible two or more substituents selected from hydroxy, amino or C^alkyl-; Het13 represents a heterocycle selected from furanyl, piperazinyl, 1,1-dioxothiomorpholinyl, piperazininonyl, piperidinyl, tetrahydro-l,l-dioxido-
2H-thiopyranyl, piperidinonyl, morpholinyl, piperazinyl or pyrrolidinyl; Het14 and Het15 each independently represent a heterocycle selected from morpholinyl, piperazinyl, piperidinyl or pyrrolidinyl wherein said Het14 and Het15 are optionally substituted with one or where possible two or more substituents selected from hydroxy, amino or C1-4alkyl;
Het16 represents a heterocycle selected from piperidinyl or pyrrolidinyl;
Het20 represents pyrrolidinyl, 2-pyrrolidinonyl, piperidinyl or hydroxy-pyrrolidinyl, preferably pyrrolidinyl or hydroxy-pyrrolidinyl; Het21 represents pyrrolidinyl or hydroxy-pyrrolidinyl; Het22 represents pyrrolidinyl, piperazinyl or piperidinyl;
Het23 and Het25 each independently represents a heterocycle selected from morpholinyl, pyrrolidinyl, piperazinyl or piperidinyl wherein said Het23 is optionally substituted with one or where possible two or more substituents selected from C1-4alkyl, C3-6cycloalkyl, hydroxy-C^alkyl-, C^alkyloxyCi^alkyl or polyhydroxy- C1-4alkyl-;
Het24 represents morpholinyl, pyrrolidinyl, piperazinyl or piperidinyl; Ar4, Ar5 or Ar6 each independently represent phenyl optionally substituted with nitro, cyano, C1-4alkylsulfonyl-, Ci_4alkylsulfonylamino-, aminosulfonylamino-, hydroxy-C1-4alkyl, aminosulfonyl-, hydroxy-, C1-4alkyloxy- or C1-4alkyl, preferably Ar4 or Ar5 each independently represent phenyl optionally substituted with cyano; further characterised in that either
Y represents -C1-2alkyl-NR23-CO-CR16R17-NH-;
Het1 represents 2-bora-l,3-dioxolanyl optionally substituted with one or where possible two, three, four or more substituents selected from amino, Chalky], hydroxy-Ci^alkyl-, phenyl, phenyl-C1-4alkyl-, Ci-4alkyl-oxy-Ci-4alkyl-, mono- or di(Ci-4alkyl)amino- or amino-carbonyl-; R13 represents Ci^alkyloxycarbonyl optionally substituted with phenyl or R13 represents Ar6-sulfonyl or Het24-C1-4alkylcarbonyl; or R4 represents Ci_4alkyloxy substituted with at least one substituent selected from
Ci.4alkyloxy-C1-4alkyloxy-, NR37R38-carbonyloxy-, Het5-carbonyloxy-, NR7R8, NR9R10-carbonyl-, Het3-carbonyl-, Het13-oxy- or Het2-; wherein R8 represents Het7-aminocarbonyl-; Het9-oxycarbonyl-; Het10-sulfonyl-;
Ci-4alkyloxycarbonyl; mono- or di(Ci-4alkyl)aminocarbonyl-; mono- or di(Ci-4alkyl)aminocarbonyl substituted with Ci-4alkylsulfonyl-; or
C1-4alkylcarbonyl optionally substituted with one or more substituents selected from C]-4alkylsulfonyl, hydroxy- and Ci-4alkyloxy-; or R8 represents Ci-4alkyl substituted with one or more substituents selected from hydroxy Ci-4alkylsulfonyl-, NR25R26, aminocarbonyloxy-, C1-4alkylcarbonyloxy-, aminocarbonyl-, C]-4alkyloxy-C1-4alkyloxy-, and
Het11;
Het13 represents Q-όalkyloxycarbonyl optionally substituted with phenyl or R13 represents Ar6-sulfonyl or Het24-Ci-4alkylcarbonyl; in particular morpholinyl-C1-4alkyl; and Het2 represents a heterocycle selected from morpholinyl, piperazinyl, piperidinyl, pyrrolidinyl or thiomorpholinyl said Het2 substituted with one or where possible two or more substituents selected from C1-4alkyl- substituted with one or more substituents selected from NR27R28, Ci-4alkylsulfonyl, aminocarbonyloxy-, aminocarbonyl- and mono- or di(Ci_4alkyl)aminocarbonyl-; or
C1-4alkyloxy- optionally substituted with C^alkyloxy-; or C1-4alkyloxycarbonyl optionally substituted with one or more substituents selected from hydroxy, Ci_4alkyloxy- and C^alkylsulfonyl-; or C^alkylcarbonyl optionally substituted with one or more substituents selected from hydroxy-, C^alkyloxy- and Ci-4alkylsulfonyl-; or Het2 represents 1,1-dioxothiomorpholinyl optionally substituted with C1-4alkyl- optionally substituted with one or more substituents selected from NR27R28, C1-4alkylsulfonyl, aminocarbonyloxy-, aminocarbonyl- and mono- or di(Ci-4alkyl)aminocarbonyl-; or C1-4alkyloxy- optionally substituted with Ci_4alkyloxy-; or
Ci_4alkyloxycarbonyl optionally substituted with one or more substituents selected from hydroxy, Ci_4alkyloxy- and C].4alkylsulfonyl-; or C^alkylcarbonyl optionally substituted with one or more substituents selected from hydroxy-, Ci-4alkyloxy- and C1-4alkylsulfonyl-.
As used in the foregoing definitions and hereinafter, - halo is generic to fluoro, chloro, bromo and iodo;
- C1 2alkyl defines methyl or ethyl;
- C1 3alkyl defines straight and branched chain saturated hydrocarbon radicals having from 1 to 3 carbon atoms such as, for example, methyl, ethyl, propyl and the like;
- C1 4alkyl defines straight and branched chain saturated hydrocarbon radicals having from 1 to 4 carbon atoms such as, for example, methyl, ethyl, propyl, butyl, 1-methylethyl, 2-methylpropyl, 2,2-dimethylethyl and the like;
- Cj.galkyl defines straight and branched chain saturated hydrocarbon radicals having from 1 to 5 carbon atoms such as, for example, methyl, ethyl, propyl, butyl, pentyl, 1-methylbutyl, 2,2-dimethylpropyl, 2,2-dimethylethyl and the like; - C^alkyl is meant to include C1-5alkyl and the higher homologues thereof having 6 carbon atoms such as, for example hexyl, 1,2-dimethylbutyl, 2-methylpentyl and the like;
- C1 7alkyl is meant to include Ci-6alkyl and the higher homologues thereof having 7 carbon atoms such as, for example 1,2,3-dimethylbutyl, 1, 2-methylpentyl and the like; - C3_9alkyl defines straight and branched chain saturated hydrocarbon radicals having from 3 to 9 carbon atoms such as propyl, butyl, pentyl, hexyl, heptyl, octyl, nonyl and the like;
- C2-4alkenyl defines straight and branched chain hydrocarbon radicals containing one double bond and having from 2 to 4 carbon atoms such as, for example vinyl, 2-propenyl, 3-butenyl, 2-butenyl and the like;
- C3_9alkenyl defines straight and branched chain hydrocarbon radicals containing one double bond and having from 3 to 9 carbon atoms such as, for example 2-propenyl, 3-butenyl, 2-butenyl, 2-pentenyl, 3-pentenyl, 3-methyl-2-butenyl, 3-hexenyl and the like; - C2-6alkynyl defines straight and branched chain hydrocarbon radicals containing one triple bond and having from 2 to 6 carbon atoms such as, for example, 2-propynyl, 3-butynyl, 2-butynyl, 2-pentynyl, 3-pentynyl, 3-methyl-2-butynyl, 3-hexynyl and the like;
- C3_6cycloalkyl is generic to cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl; - Ci_4alkyloxy defines straight or branched saturated hydrocarbon radicals such as methoxy, ethoxy, propyloxy, butyloxy, 1-methylethyloxy, 2-methylpropyloxy and the like; - Ci_6alkyloxy is meant to include Ci^alkyloxy and the higher homologues such as methoxy, ethoxy, propyl oxy, butyl oxy, 1-methylethyloxy, 2-methylpropyloxy and the like;
- polyhydroxy-C1-4alkyl is generic to a C^alkyl as defined hereinbefore, having two, three or where possible more hydroxy substituents, such as for example trifluoromethyl.
As used in the foregoing definitions and hereinafter, the term formyl refers to a radical of formula -CH(=O). When X1 represents the divalent radical -0-N=CH-, said radical is attached with the carbon atom to the R3, R4 bearing cyclic moiety of the compounds of formula (I) and when X2 represents the divalent radical -0-N=CH-, said radical is attached with the carbon atom to the R1, R2 bearing phenyl moiety of the compounds of formula (I).
The heterocycles as mentioned in the above definitions and hereinafter, are meant to include all possible isomeric forms thereof, for instance pyrrolyl also includes 2H-pyrrolyl; triazolyl includes 1,2,4-triazolyl and 1,3,4-triazolyl; oxadiazolyl includes 1,2,3-oxadiazolyl, 1,2,4-oxadiazolyl, 1,2,5-oxadiazolyl and 1,3,4-oxadiazolyl; thiadiazolyl includes 1,2,3-thiadiazolyl, 1,2,4-thiadiazolyl, 1,2,5-thiadiazolyl and 1,3,4-thiadiazolyl; pyranyl includes 2H-pyranyl and 4H-pyranyl.
Further, the heterocycles as mentioned in the above definitions and hereinafter may be attached to the remainder of the molecule of formula (I) through any ring carbon or heteroatom as appropriate. Thus, for example, when the heterocycle is imidazolyl, it may be a 1-imidazolyl, 2-imidazolyl, 3-imidazolyl, 4-imidazolyl and 5-imidazolyl; when it is thiazolyl, it may be 2-thiazolyl, 4-thiazolyl and 5-thiazolyl; when it is triazolyl, it may be 1,2,4-triazol-l-yl, l,2,4-triazol-3-yl, l,2,4-triazol-5-yl, 1,3,4-triazol- 1-yl and l,3,4-triazol-2-yl; when it is benzothiazolyl, it may be 2-benzothiazolyl, 4-benzothiazolyl, 5-benzothiazolyl, 6-benzothiazolyl and 7-benzothiazolyl.
The pharmaceutically acceptable addition salts as mentioned hereinabove are meant to comprise the therapeutically active non-toxic acid addition salt forms which the compounds of formula (I) are able to form. The latter can conveniently be obtained by treating the base form with such appropriate acid. Appropriate acids comprise, for example, inorganic acids such as hydrohalic acids, e.g. hydrochloric or hydrobromic acid; sulfuric; nitric; phosphoric and the like acids; or organic acids such as, for example, acetic, propanoic, hydroxyacetic, lactic, pyruvic, oxalic, malonic, succinic (i.e. butane-dioic acid), maleic, fumaric, malic, tartaric, citric, methanesulfonic, ethanesulfonic, benzenesulfonic, p-toluenesulfonic, cyclamic, salicylic, ^-aminosalicylic, pamoic and the like acids.
The pharmaceutically acceptable addition salts as mentioned hereinabove are meant to comprise the therapeutically active non-toxic base addition salt forms which the compounds of formula (I) are able to form. Examples of such base addition salt forms are, for example, the sodium, potassium, calcium salts, and also the salts with pharmaceutically acceptable amines such as, for example, ammonia, alkylamines, benzathine, N-methyl-D-glucamine, hydrabamine, amino acids, e.g. arginine, lysine.
Conversely said salt forms can be converted by treatment with an appropriate base or acid into the free acid or base form.
The term addition salt as used hereinabove also comprises the solvates which the compounds of formula (I) as well as the salts thereof, are able to form. Such solvates are for example hydrates, alcoholates and the like.
The term stereochemically isomeric forms as used hereinbefore defines the possible different isomeric as well as conformational forms which the compounds of formula (I) may possess. Unless otherwise mentioned or indicated, the chemical designation of compounds denotes the mixture of all possible stereochemically and conformationally isomeric forms, said mixtures containing all diastereomers, enantiomers and/or conformers of the basic molecular structure. All stereochemically isomeric forms of the compounds of formula (I) both in pure form or in admixture with each other are intended to be embraced within the scope of the present invention.
Some of the compounds of formula (I) may also exist in their tautomeric forms. Such forms although not explicitly indicated in the above formula are intended to be included within the scope of the present invention.
The N-oxide forms of the compounds of formula (I) are meant to comprise those compounds of formula (I) wherein one or several nitrogen atoms are oxidized to the so-called iV-oxide.
A first group of compounds according to the present invention consists of those compounds of formula (I) wherein one or more of the following restrictions apply; Z represents NH; Y represents -C3.9alkyl-, -C2_9alkenyl-, -Ci-salkyl-oxy-Ci.salkyl-,
Figure imgf000015_0001
-Ci-ealkyl-NH-CO-, -NH-CO-Ci-βalkyl-, -CO-Ci_7alkyl-, -C1-7alkyl-CO-, Ci.6alkyl-CO-Ci_6alkyl, -Ci.2alkyl-NR23-CO-CR16R17-NH-, -C1-2alkyl-CO-NH-CR18R19-CO-, -Ci-2alkyl-CO-NR20-C1-3alkyl-CO-, -Ci.2alkyl-NR21-CH2-CO-NH-Ci.3alkyl-,
-NR22-CO-d.3alkyl-NH-, -C1-3alkyl-NH-CO-Het20-, C,.2alkyl-CO-Het21-CO-, or -Het22-CH2-CO-NH-C1-3alkyl-;
X1 represents O, -O-C1-2alkyl-, -0-N=CH-, NR11 or -NRn-Ci_2alkyl-; in a particular embodiment X1 represents O, -O-C1-2alkyl- or NRn-Ci_2alkyl; X2 represents a direct bond, C] -2alkyl, O, -O-C1-2alkyl-, CO, -CO-d.2alkyl-, -0-N=CH- , NR12 or NR12-C1-2alkyl-; in a particular embodiment X2 represents a direct bond, -0-, -O-Ci-2alkyl, -CO-Ci-2alkyl- or NR12-C1-2alkyl-;
R1 represents hydrogen, cyano, halo or hydroxy, preferably halo;
R2 represents hydrogen, cyano, halo, hydroxy, hydroxycarbonyl-, Ci_4alkyloxycarbonyl-, Het16-carbonyl-, C1-4alkyl-, C2-6alkynyl-, Ar5, Het1 or dihydroxyborane ;
R3 represents hydrogen, cyano, halo, hydroxy, formyl, C1-6alkoxy-, Ci-6alkyl-,
Ci-βalkoxy- substituted with halo, or R3 represents d_4alkyl substituted with one or where possible two or more substituents selected from hydroxy or halo; R4 represents Ar4-C1-4alkyloxy-, Ci-4alkyloxy- or R4 represents Ci-4alkyloxy substituted with one or where possible two or more substituents selected from hydroxy-, halo, Ci_4alkyloxy-, C1-4alkyloxy-C1-4alkyloxy-, NR37R38-carbonyloxy-, Het5- carbonyloxy-, NR7R8, NR9R10-carbonyl-, Het3-carbonyl-, Het13-oxy- or Het2-;
R7 represents hydrogen or C1-4alkyl; R8 represents C3-6cycloalkyl, Het6-carbonyl-, Het7-aminocarbonyl-, Het8,
Het9-oxycarbonyl-, Het10-sulfonyl-, mono- or di(C1-4alkyl)aminocarbonyl-, mono- or di(C1-4alkyl)aminocarbonyl substituted with Ci-4alkylsulfonyl-, or Ci-4alkylcarbonyl optionally substituted with one or more substituents selected from C1-4alkylsulfonyl, hydroxy- and Ci-4alkyloxy-, or R8 represents Ci-4alkyl substituted with one or more substituents selected from
Ci-4alkylsulfonyl-, NR R , aminocarbonyloxy-, aminocarbonyl-, d.4alyloxy-d-4alkyloxy-, and Het11;
R9 represents hydrogen or Ci_4alkyl-;
R10 represents Het4 or Ci_4alkyl- substituted with Ci-4alkylsulfonyl-, ; R11 represents hydrogen, Ci-4alkyl- or Ci-4alkyl-oxy-carbonyl-;
R12 represents hydrogen, Ci-4alkyl-, d^alkyloxycarbonyl- or Ci_6alkyloxycarbonyl- substituted with phenyl; R13 represents hydrogen, Het14-C].4alkyl,
Figure imgf000016_0001
optionally substituted with phenyl or R13 represents Ar6-sulfonyl or Het24-C]_4alkylcarbonyl; in particular morpholinyl-C1-4alkyl;
R14 and R15 are each independently selected from hydrogen, Ci-4alkyl, Het15-C1-4alkyl- or C1_4alkyloxyC1.4alkyl-;;
R16 and R17 each independently represents hydrogen, C1-4alkyl or C]_4alkyl substituted with hydroxy- or phenyl; or R16 and R17 taken together with the carbon atom to which they are attached form a C3-6C ycloalkyl;
R18 represents hydrogen or C1-4alkyl optionally substituted with hydroxy or phenyl; R19 represents hydrogen or C1-4alkyl, in particular hydrogen or methyl, even more particular hydrogen;
R20 represents hydrogen or Ci-4alkyl, in particular hydrogen or methyl; R21 represents hydrogen, C1-4alkyl, Het23-C1-4alkylcarbonyl- or
R21 represents mono-or di(C1-4alkyl)amino-C1-4alkyl-carbonyl- optionally substituted with hydroxy, pyrimidinyl, dimethylamine or C1 4alkyloxy;
R22 represents hydrogen or C1-4alkyl optionally substituted with hydroxy or
C1-4alkyloxy; R23 represents C].4alkyl optionally substituted with hydroxy-, C1-4alkyloxy- or Het23;
R23 may also represent hydrogen when R16 and R17 taken together with the carbon atom to which they are attached form a C3-6cycloalkyl;
R25 and R26 each independently represent hydrogen, C1-4alkyl, C1 4alkylsulfonyl-, aminocarbonyl-, mono- or di(C1-4alkyl)aminocarbonyl-, C]-4alkylcarbonyl-,
C1-4alkyloxycarbonyl- or C1-4alkyl substituted with one or more substituents selected from C]_4alkylsulfonyl-, hydroxy- and C1-4alkyloxy-, in particular R25 and R26 each independently represent hydrogen, C1-4alkyl, C1-4alkylsulfonyl-, aminocarbonyl-, mono- or di(C1-4alkyl)aminocarbonyl- or C1-4alkylcarbonyl-; R27 and R28 each independently represent hydrogen, C^alkyl, C].4alkylsulfonyl-, aminocarbonyl-, mono- or di(C1-4alkyl)aminocarbonyl-, C1-4alkylcarbonyl-,
C1-4alkyloxycarbonyl- or C1-4alkyl substituted with one or more substituents selected from C1-4alkylsulfonyl-, hydroxy- and C1-4alkyloxy-;
R29 and R30 each independently represent hydrogen, aminosulfonyl, aminocarbonyl, mono- or di(C1-4alkyl)aminocarbonyl-, mono- or di(C].4alkyl)aminosulfonyl-, or
C1-4alkyl- optionally substituted with one or more substituents selected from
NR31R32, C].4alkylsulfonyl, aminocarbonyloxy-, hydroxy-, C1-4alkyloxy-, aminocarbonyl- and mono- or di(C].4alkyl)aminocarbonyl-, or
C^alkyloxycarbonyl optionally substituted with one or more substituents selected from hydroxy, C1-4alkyloxy- and C1-4alkylsulfonyl-, or C^alkylcarbonyl optionally substituted with one or more substituents selected from hydroxy-, C1-4alkyloxy- and C^alkylsulfonyl-; R31 and R32 each independently represent hydrogen, d.4alkyl, d-4alkylsulfonyl-, aminocarbonyl-, mono- or di(C1-4alkyl)aminocarbonyl-, C1-4alkylcarbonyl-,
Figure imgf000017_0001
or Ci_4alkyl substituted with one or more substituents selected from Ci^alkylsulfonyl-, hydroxy- and C1-4alkyloxy-; R33 represents hydrogen or C1-4alkyl; R34 represents Ci-4alkylsulfonyl-, aminocarbonyl-, mono- or di(C^4alkyl)aminocarbonyl-, C1-4alkylcarbonyl-, C1-4alkyloxycarbonyl- or C1-4alkyl substituted with one or more substituents selected from C1-4alkylsulfonyl-
, hydroxy- and C^alkyloxy-; R35 represents hydrogen or C1-4alkyl; R36 represents C1-4alkylsulfonyl-, aminocarbonyl-, mono- or di(Ci-4alkyl)aminocarbonyl-, C1-4alkylcarbonyl-, Ci-4alkyloxycarbonyl- or Ci.4alkyl substituted with one or more substituents selected from C1-4alkylsulfonyl-
, hydroxy- and Ci_4alkyloxy-; R37 and R38 each independently represent hydrogen, d_4alkyl, C1-4alkylsulfonyl-, Het12 or C1-4alkyl substituted with one or more substituents selected from Ci- 4alkylsulfonyl-, hydroxy- and C1-4alkyloxy-; R39 and R40 each independently represent aminosulfonyl, aminocarbonyl, mono- or di(C1-4alkyl)aminocarbonyl-, mono- or di(C1-4alkyl)aminosulfonyl-, or
Ci-4alkyl- substituted with one or more substituents selected from NR31R32,
C1-4alkylsulfonyl, aminocarbonyloxy-, hydroxy-, Ci-4alkyloxy-, aminocarbonyl- and mono- or di(Ci_4alkyl)aminocarbonyl-, or Ci_4alkyloxycarbonyl optionally substituted with one or more substituents selected from hydroxy, Ci-4alkyloxy- and Ci-4alkylsulfonyl-, or
Ci^alkylcarbonyl optionally substituted with one or more substituents selected from hydroxy-, Ci-4alkyloxy- and C1-4alkylsulfonyl-;
Het1 represents thiazolyl or 2-bora-l,3-dioxolanyl wherein said Het1 is optionally substituted with one or where possible two, three, four or more substituents selected from amino, Ci-4alkyl, hydroxy-C1-4alkyl-, phenyl, phenyl-d^alkyl-, d-4alkyl-oxy-d_4alkyl-, mono- or di(d-4alkyl)amino- or amino-carbonyl-; Het2 represents a heterocycle selected from tetrahydropyranyl, tetrahydrofuranyl, furanyl, 1,1-dioxothiomorpholinyl, piperazininonyl, tetrahydro-l,l-dioxido-2H- thiopyranyl, piperidinonyl, azetidinyl or 2-azetidinonyl wherein said Het2 is optionally substituted with one or where possible two or more substituents selected from hydroxy, amino, NR29R30, aminocarbonyl, mono- or
Figure imgf000018_0001
Ci 4alkylsulfonyl or
Ci-4alkyl- optionally substituted with one or more substituents selected from
NR27R28, C1-4alkylsulfonyl, aminocarbonyloxy-, aminocarbonyl- and mono- or di(C1-4alkyl)aminocarbonyl-, or Ci_4alkyloxy- optionally substituted with C1-4alkyloxy-, or
C1-4alkyloxycarbonyl optionally substituted with one or more substituents selected from hydroxy, Ci_4alkyloxy- and Ci-4alkylsulfonyl-, or
C^alkylcarbonyl optionally substituted with one or more substituents selected from hydroxy-, Ci_4alkyloxy- and Ci-4alkylsulfonyl-; or Het2 represents a heterocycle selected from morpholinyl, piperazinyl, piperidinyl or pyrrolidinyl wherein said moφholinyl, piperazinyl, piperidinyl or pyrrolidinyl are optionally substituted with one or where possible two or more substituents selected from
Ci-4alkyl- optionally substituted with one or more substituents selected from NR27R28, Ci-4alkylsulfonyl, aminocarbonyloxy-, aminocarbonyl- and mono- or di(Ci-4alkyl)aminocarbonyl-, or
C1-4alkyloxy- optionally substituted with C1-4alkyloxy-, or
C^alkyloxycarbonyl optionally substituted with one or more substituents selected from hydroxy, C1-4alkyloxy- and C1_4alkylsulfonyl-, or C1-4alkylcarbonyl optionally substituted with one or more substituents selected from hydroxy-, Ci_4alkyloxy- and C1-4alkylsulfonyl-; Het3 represents a heterocycle selected from tetrahydropyranyl, tetrahydrofuranyl, furanyl, 1,1-dioxothiomorpholinyl, piperazininonyl, tetrahydro-l,l-dioxido-2H- thiopyranyl, piperidinonyl, azetidinyl or 2-azetidinonyl wherein said Het3 is optionally substituted with one or where possible two or more substituents hydroxy-, amino, C1-4alkyl-, C^cycloalkyl-Ci^alkyl-, aminosulfonyl-, mono- or di(C1.4alkyl)aminosulfonyl-, amino-C1-4alkyl-, Mono- or di(Ci-4alkyl)amino-
C1-4alkyl, NR35R36, C1-4alkyl-sulfonyl-C1-4alkyl- or Ci-4alkyloxy- optionally substituted with C1-4alkyloxy- or hydroxy; or Het3 represents a heterocycle selected from moφholinyl, piperazinyl, piperidinyl or pyrrolidinyl wherein said Het3 is optionally substituted with one or where possible two or more substituents selected from NR35R36, C1-4alkyl-sulfonyl-C1-4alkyl- or
Ci_4alkyloxy- optionally substituted with C^alkyloxy- or hydroxy; Het4 represents a heterocycle selected from moφholinyl, piperazinyl, piperidinyl, furanyl, pyrazolyl, dioxolanyl, thiazolyl, oxazolyl, imidazolyl, isoxazolyl, oxadiazolyl, pyridinyl or pyrrolidinyl wherein said Het4 is substituted with one or where possible two or more substituents selected from Ci-4alkyl-sulfonyl- Ci_4alkyl-, Ci-4alkyloxy- optionally substituted with Ci-4alkyloxy- or hydroxy; Het5 represents a heterocycle selected from furanyl, piperazinyl,
1,1-dioxothiomorpholinyl, piperazininonyl, piperidinyl, tetrahydro-l,l-dioxido- 2H-thiopyranyl, piperidinonyl, morpholinyl or pyrrolidinyl wherein said Het5 is optionally substituted with hydroxy, amino, mono- or di(C]_4alkyl)-amino-, Ci-4alkyl,
Het6 and Het7 each independently represents a heterocycle selected from piperazinyl, piperidinyl or pyrrolidinyl wherein said heterocycles are optionally substituted with one or more substituents selected from hydroxy-, amino-, hydroxy-C1-4alkyl-,
C^alkyloxy-C^alkyl- and C1-4alkyl-;
Het represents a heterocycle selected from tetrahydropyranyl, tetrahydrofuranyl, 1 , 1 -dioxothiomorpholinyl, piperazininonyl, tetrahydro- 1 , 1 -dioxido-2H- thiopyranyl, piperidinonyl, azetidinyl or 2-azetidinonyl wherein said Het8 is optionally substituted with aminosulfonyl, aminocarbonyl, mono- or di(Ci-4alkyl)aminocarbonyl-, mono- or di(C].4alkyl)aminosulfonyl-, or C1-4alkyl- optionally substituted with one or more substituents selected from amino, mono- or di(C]_4alkyl)amino-, NR33R34, C].4alkylsulfonyl, aminocarbonyloxy-, hydroxy-, C1-4alkyloxy-, aminocarbonyl- and mono- or di(C]_4alkyl)aminocarbonyl-, or
C]_4alkyloxycarbonyl optionally substituted with one or more substituents selected from hydroxy-, C^alkyloxy- and C].4alkylsulfonyl-, or C1-4alkylcarbonyl optionally substituted with one or more substituents selected from hydroxy-, C].4alkyloxy- and C].4alkylsulfonyl-; or Het represents a heterocycle selected from furanyl, piperidinyl or piperazinyl wherein said Het8 is substituted with aminocarbonyl, mono- or di(C1-4alkyl)aminocarbonyl-, mono- or di(C]-4alkyl)aminosulfonyl-, or C1-4alkyl- substituted with one or more substituents selected from NR33R34, C].4alkylsulfonyl, aminocarbonyloxy-, hydroxy-, Ci-4alkyloxy-, aminocarbonyl- and mono- or di(C].4alkyl)aminocarbonyl-, or
C].4alkyloxycarbonyl optionally substituted with one or more substituents selected from hydroxy-, Ci.4alkyloxy- and C].4alkylsulfonyl-, or C].4alkylcarbonyl optionally substituted with one or more substituents selected from hydroxy-, Q^alkyloxy- and Ci_4alkylsulfonyl-; Het9 and Het10 each independently represents a heterocycle selected from piperazinyl, piperidinyl or pyrrolidinyl wherein said heterocycles are optionally substituted with one or more substituents selected from hydroxy-, amino, hydroxy-C1-4alkyl-, C1-4alkyloxy-Ci-4alkyl- and C1-4alkyl-;
Het11 represents 2-imidazolidinonyl- or ^ \ ;
Het12 represents a heterocycle selected from morpholinyl, piperazinyl, piperidinyl or pyrrolidinyl wherein said Het12 is optionally substituted with one or where possible two or more substituents selected from hydroxy-, amino or C^alkyl-; Het13 represents a heterocycle selected from furanyl, piperazinyl, 1,1- dioxothiomorpholinyl, piperazininonyl, piperidinyl, tetrahydro-l,l-dioxido-2H- thiopyranyl, piperidinonyl, morpholinyl, piperazinyl or pyrrolidinyl Het16 represents a heterocycle selected from piperidinyl or pyrrolidinyl;
Het20 represents pyrrolidinyl, 2-pyrrolidinonyl, piperidinyl or hydroxy-pyrrolidinyl, preferably pyrrolidinyl or hydroxy-pyrrolidinyl; Het21 represents pyrrolidinyl or hydroxy-pyrrolidinyl; Het22 represents pyrrolidinyl, piperazinyl or piperidinyl; Het23 represents a heterocycle selected from morpholinyl, pyrrolidinyl, piperazinyl or piperidinyl wherein said Het23 is optionally substituted with one or where possible two or more substituents selected from C^alkyl, C3-6C ycloalkyl, hydroxy- C1-4alkyl-, C1-4alkyloxyC1-4alkyl or polyhydroxy-C1-4alkyl-;
Ar4, Ar5 or Ar6 each independently represent phenyl optionally substituted with nitro, cyano, C1-4alkylsulfonyl-, C1-4alkylsulfonylamino-, aminosulfonylamino-, hydroxy-C1-4alkyl, aminosulfonyl-, hydroxy-, C^alkyloxy- or C1-4alkyl, preferably Ar4 or Ar5 each independently represent phenyl optionally substituted with cyano; further characterised in that either Y represents -C1-2alkyl-NR23-CO-CR16R17-NH-;
Het1 represents 2-bora-l,3-dioxolanyl optionally substituted with one or where possible two, three, four or more substituents selected from amino, C1-4alkyl, hydroxy-C1-4alkyl-, phenyl, phenyl-C1-4alkyl-, C1-4alkyl-oxy-C1-4alkyl-, mono- or di(C1-4alkyl)amino- or amino-carbonyl-; R13 represents C1-6alkyloxycarbonyl optionally substituted with phenyl or R13 represents Ar6-sulfonyl or Het24-C1-4alkylcarbonyl; or
R4 represents C^alkyloxy substituted with at least one substituent selected from C1-4alkyloxy-C!_4alkyloxy-, NR R -carbonyloxy-, Het -carbonyloxy-, NR7R8, NR9R10-carbonyl-, Het3-carbonyl-, Het13-oxy- or Het2-. Another group of compounds according to the present invention consists of those compounds of formula (I) wherein one or more of the following restrictions apply; Z represents NH;
Y represents -C3-9alkyl-, -C1-5alkyl-NR13-C1-5alkyl-, -d-5alkyl-NR14-CO-C1-5alkyl-, -d_6alkyl-CO-NH-, -d-ealkyl-NH-CO-, -C^alkyl-NR^-CO-CR^R^-NH-,
-C1-2alkyl-NR21-CH2-CO-NH-C1-3alkyl or C1-3alkyl-NH-CO-Het20-; in particular Y represents -C3-9alkyl-,
Figure imgf000021_0001
-C1-6alkyl-CO-NH-, -C1-6alkyl-NH-CO-, -C1-2alkyl-NR23-CO-CR16R17-NH- or d.3alkyl-NH-CO-Het20- X1 represents a direct bond, O, -O-d_2alkyl-, NR11, or -NRπ-C1-2alkyl-;
X2 represents a direct bond, -C1-2alkyl-, CO-C1-2alkyl or NR12-C1-2alkyl-; in particular
X2 represents a direct bond, -C1-2alkyl- or NR12-C1-2alkyl-; R1 represents hydrogen, cyano, halo or hydroxy;
R2 represents hydrogen, halo, cyano, C2-6alkynyl, hydroxy, hydroxycarbonyl, d_4alkyloxycarbonyl- or Het1; in particular R2 represents hydrogen, halo, cyano, acetylene (-C≡CH), hydroxy, hydroxycarbonyl, C1-4alkyloxycarbonyl- or Het1; more in particular R2 represents hydrogen, halo, cyano, hydroxy, hydroxycarbonyl,
C1-4alkyloxycarbonyl- or Het1
R3 represents hydrogen, cyano, halo, hydroxy, formyl, C1-6alkyloxy or C1-6alkyloxy- substituted with halo;
R4 represents Ar4-d-4alkyloxy, C1-4alkyloxy-, or C1-4alkyloxy- substituted with one or where possible two or more substituents selected from hydroxy, d-4alkyloxy-,
Cϊ^alkyloxy-Ci^alkyloxy, NR7R8 or Het2; in particular R4 represents
Ar4-C1-4alkyloxy, Q^alkyloxy-, or C1-4alkyloxy- substituted with one or where possible two or more substituents selected from C1-4alkyloxy-, C1-4alkyloxy-
C1-4alkyloxy or NR7R8
R7 represents hydrogen, hydroxyC^alkyl- or C1-4alkyl; R8 represents C1-4alkyloxycarbonyl or C1-4alkyl- substituted with one or more substituents selected from C1-4alkylsulfonyl-, C^alkylcarbonyloxy Or NR25R26; in particular R represents C1-4alkyl- substituted with one or more substituents selected from C1-4alkylsulfonyl- or NR25R26; R11 represents hydrogen, C1-4alkyloxycarbonyl or C^alkyl; in particular R11 represents hydrogen or C^alkyl; R12 represents hydrogen or C1-4alkyl; R13 represents d^alkyloxycarbonyl optionally substituted with phenyl or R13 represents Ar6-sulfonyl or Het24-d_4alkylcarbonyl; R14 and R15 each independently represent hydrogen or C1-4alkyl; in particular R14 and
R15 each independently represent hydrogen; R16 and R17 each independently represent hydrogen or C1-4alkyl optionally substituted with C3_6cycloalkyl or R16 and R17 taken together with the carbon atom to which they are attached form a C3.6cycloalkyl; in a particular embodiment R16 and R17 taken together with the carbon atom to which they are attached form a
C3_6cycloalkyl; R21 represents hydrogen or C1-4alkyloxycarbonyl; in particular R21 represents
C ] -4alkyloxyc arbonyl R23 represents C]-4alkyl optionally substituted with hydroxy-, C1-4alkyloxy- or Het25;
R23 may also represent hydrogen when R16 and R17 taken together with the carbon atom to which they are attached form a C3-6C ycloalkyl; R25 and R26 each independently represent hydrogen, C1-4alkyl, C1-4alkylsulfonyl,
C1 -4alkyloxyc arbonyl or C1-4alkylcarbonyl; in particular R25 and R26 each independently represents hydrogen or Ci-4alkylcarbonyl;
Tl 1J R
R and R each independently represent hydrogen, C1-4alkyl, C1-4alkylsulfonyl, C].4alkyloxycarbonyl or C ^alkylc arbonyl; in particular R27 and R28 each independently represent hydrogen or C]-4alkylcarbonyl; Het1 represents 2-bora-l,3-dioxolanyl- optionally substituted with one or where possible two, three, four or more substituents selected from amino, C1-4alkyl, hydroxy-C1-4alkyl-, phenyl, phenyl-C1-4alkyl, C1-4alkyloxyC1-4alkyl-, mono- or di(C1-4alkyl)amino- or aminocarbonyl-; Het2 represents 1,1-dioxothiomorpholinyl optionally substituted with
C1-4alkyloxycarbonyl or C1-4alkyl-NR27R28; or Het2 represents piperidinyl or piperazinyl substituted with C1-4alkyloxycarbonyl or -C1-4alkyl-NR27R28;
Het20 represents pyrrolidinyl, 2-pyrrolidinonyl, piperidinyl or hydroxy-pyrrolidinyl; in particular Het20 represents pyrrolidinyl, piperidinyl or hydroxy-pyrrolidinyl; more in particular Het20 represents pyrrolidinyl;
Het25 represents a heterocycle selected from morpholinyl or piperazinyl wherein said heterocycle is optionally substituted with C1-4alkyl, hydroxy-Ci^alkyl,
C^alkyloxy-C^alkyl or polyhydroxy-C1-4alkyl; or Ar4, Ar5 or Ar6 each independently represents phenyl optionally substituted with nitro, cyano, hydroxy, hydroxyd-4alkyl, C1-4alkyl or C1-4alkyloxy; further characterised in that either Y represents -C1-2alkyl-NR23-CO-CR16R17-NH-; or
R4 represents C^alkyloxy substituted with at least one substituent selected from Ci_4alkyloxy-CMalkyloxy-, NR7R8 or Het2. Another group of compounds according to the present invention consists of those compounds of formula (I) wherein one or more of the following restrictions apply; Z represents NH;
Y represents -C3-9alkyl-, -Ci_5alkyl-NR13-C1-5alkyl-, -Ci-5alkyl-NR14-CO-Ci.5alkyl-, -Ci-ealkyl-CO-NH-, -Ci.ealkyl-NH-CO-, -Ci-2alkyl-NR23-CO-CR16R17-NH-,
-C1-2alkyl-NR21-CH2-CO-NH-Ci-3alkyl or C1-3alkyl-NH-CO-Het20-; in particular Y represents -C3-9alkyl-,
Figure imgf000023_0001
-Ci-6alkyl-CO-NH-, -Ci_6alkyl-NH-CO-, -Ci-2alkyl-NR23-CO-CR16R17-NH- or
C1-3alkyl-NH-CO-Het20- X1 represents a direct bond, O, -O-C1-2alkyl-, NR1 \ or -NRu-C1-2alkyl-;
X2 represents a direct bond, -C^alkyl-, CO-Ci_2alkyl or NR12-C1-2alkyl-; in particular
X2 represents a direct bond, -Ci-2alkyl- or NR12-Ci-2alkyl-; R1 represents hydrogen or halo;
R2 represents hydrogen, halo, C2_6alkynyl, cyano or Het1; in particular R2 represents hydrogen, halo, C2-6alkynyl or Het1; more in particular R2 represents hydrogen, halo, acetylene or Het1; or R2 represents hydrogen, halo, cyano or Het1; R3 represents hydrogen; R4 represents Ar4-C1_4alkyloxy, C1-4alkyloxy-, or C1-4alkyloxy- substituted with one or where possible two or more substituents selected from hydroxy, C1-4alkyloxy-, C1-4alkyloxy-Ci-4alkyloxy, NR7R8 or Het2; in particular R4 represents
Ar4-Ci-4alkyloxy, C1-4alkyloxy-, or C^alkyloxy- substituted with one or where possible two or more substituents selected from C1-4alkyloxy-, Ci-4alkyloxy-
Ci-4alkyloxy or NR7R8 R7 represents hydrogen or Ci_4alkyl; R8 represents Ci-4alkyloxycarbonyl or C1-4alkyl- substituted with one or more substituents selected from C1-4alkylsulfonyl-, hydroxy, C^alkylcarbonyloxy or
NR25R26; in particular R8 represents Ci-4alkyl- substituted with one or more substituents selected from C1-4alkylsulfonyl- or NR25R26; R11 represents hydrogen or C1-4alkyl; R12 represents hydrogen or Ci-4alkyl;
R13 represents Ar6-sulfonyl or Ci-6alkyloxycarbonyl optionally substituted with phenyl;
R14 and R15 represent hydrogen;
R16 and R17 each independently represent hydrogen or Ci-4alkyl optionally substituted with C3.6cycloalkyl or R16 and R17 taken together with the carbon atom to which they are attached form a C3.6cycloalkyl; in a particular embodiment R16 and R17 taken together with the carbon atom to which they are attached form a
C3-6cycloalkyl; R21 represents hydrogen or C1-4alkyloxycarbonyl;
R23 represents Ci 4alkyl optionally substituted with hydroxy-, C^alkyloxy- or Het25; R23 may also represent hydrogen when R16 and R17 taken together with the carbon atom to which they are attached form a C3-6cycloalkyl; R25 and R26 each independently represent hydrogen or C1-4alkylcarbonyl; R27 and R28 each independently represent hydrogen or Ci-4alkylcarbonyl; Het1 represents 2-bora-l,3-dioxolanyl-; Het2 represents 1,1-dioxothiomorpholinyl, piperidinyl or piperazinyl wherein said Het2 is optionally substituted with C1-4alkyloxycarbonyl or -C1-4alkyl-NR27R28; Het20 represents pyrrolidinyl;
Het25 represents a heterocycle selected from morpholinyl or piperazinyl wherein said heterocycle is optionally substituted with C1-4alkyl, hydroxy-C1-4alkyl, Cϊ^alkyloxy-Cϊ^alkyl or polyhydroxy-C1-4alkyl; Ar4 represents phenyl; Ar5 represents phenyl; or
Ar6 represents phenyl optionally substituted with nitro; further characterised in that either
Y represents -C1-2alkyl-NR23-CO-CR16R17-NH-; or
R4 represents d-4alkyloxy substituted with at least one substituent selected from Ci^alkyloxy-Cϊ^alkyloxy-, NR7R8 or Het2; in particular C^alkyloxy substituted with C^alkyloxy-C^alkyloxy- or NR7R8.
An interesting group of compounds consists of those compounds of formula (I) wherein one or more of the following restrictions apply : Z represents NH;
Y represents -C3-9alkyl-, -C1-5alkyl-NR13-C1-5alkyl-, -C1-5alkyl-NR14-CO-C1-5alkyl-, -C1-2alkyl-NR21-H2-CO-NH-C1-3alkyl- or -C1-2alkyl-NR23-CO-CR16R17-NH-; in particular Y represents -C3-9alkyl-, -C1-5alkyl-NR13-C1-5alkyl- or -Ci_2alkyl-NR23-CO-CR16R17-NH- X1 represents O or -O-Ci_2alkyl-; inparticular X1 represents O
X2 represents a direct bond, C^alkyl, -CO-C1-2alkyl or NR12-Ci_2alkyl; in particular X2 represents a direct bond or NR12-C1-2alkyl-;
R1 represents hydrogen or halo; in particular R1 represents hydrogen; R2 represents halo, C2_6alkynyl, cyano or Het1; in particular R2 represents halo, acetylene or Het1 ; more in particular R2 represents halo or Het1 ;
R3 represents hydrogen; R4 represents Ar4-Ci_4alkyloxy-, C^alkyloxy- or Ci_4alkyloxy substituted with one or where possible two or more substituents selected from Het2, NR7R8, hydroxy and Ci_4alkyloxy-Ci_4alkyloxy-; in particular R4 represents Ar4-C1-4alkyloxy-, Ci-4alkyloxy- or Ci_4alkyloxy substituted with Ci-4alkyloxy-C1-4alkyloxy-; R7 represents hydrogen or C1-4alkyl;
R8 represents C^alkyl substituted with NR25R26 or C1-4alkylsulfonyl;
R12 represents hydrogen or C1-4alkyl-;
R13 represents Ar6-sulfonyl or C1-6alkyloxycarbonyl optionally substituted with phenyl;
R16 and R17 represents hydrogen, C1-4alkyl or R16 and R17 taken together with the carbon atom to which they are attached from a C3_6cycloalkyl;
R23 represents hydrogen or C1-4alkyl; in particular R23 represents Ci_4alkyl and R23 represents hydrogen when R16 and R17 taken together with the carbon atom to which they are attached from a C3-6cycloalkyl;
R25 and R26 each independently represent hydrogen or C1-4alkylcarbonyl; R27 and R28 each independently represent hydrogen or C1-4alkylcarbonyl;
Het1 represents 2-bora-l,3-dioxolanyl;
Het2 represents piperidinyl, piperazinyl, morpholinyl, thiomorpholinyl or
1,1-dioxothiomorpholinyl wherein said Het2 is optionally substituted with d-4alkyloxycarbonyl or NR27R28-C1-4alkyl; in particular Het2 represents 1,1-dioxothiomorpholinyl; piperidinyl substituted with C1-4alkyloxycarbonyl; or piperazinyl substituted with Q^alkyloxycarbonyl or NR27R28-C!_4alkyl-;
Ar4 represents phenyl;
Ar5 represents phenyl; or
Ar6 represents phenyl optionally substituted with nitro.
An interesting group of compounds consists of those compounds of formula (I) wherein one or more of the following restrictions apply :
Z represents NH;
Y represents -C3-9alkyl-,-C1-5alkyl-NR13-C1-5alkyl-, -C1-5alkyl-NR14-CO-C1-5alkyl-, -C1_2alkyl-NR21-CH2-CO-NH-C1-3alkyl-, C 1-6alkyl -NH-CO- or -d_2alkyl-NR23-
CO-CR16R17-NH-; in particular Y represents -C3-9alkyl-, C1-6alkyl-NH-CO- -C1-5alkyl-NR13-C1-5alkyl-, -C1-5alkyl-NR14-CO-C1-5alkyl-, or -C1 2alkyl-NR23-CO-CR16R17-NH-
X1 represents O or -O-C^alkyl-; in particular X1 represents O X2 represents a direct bond, C^alkyl, -CO-C^alkyl or NR12-C1-2alkyl; in particular X2 represents -CO-C^alkyl or NR12-C!_2alkyl-; R1 represents hydrogen, cyano or halo; in particular R1 represents hydrogen or halo, more in particular R1 represents hydrogen, fluoro or bromo;
R2 represents halo, C2-6alkynyl, cyano or Het1; in particular R2 represents halo, acetylene or Het1; more in particular R2 represents halo or Het1; R3 represents hydrogen;
R4 represents Ar4-Ci-4alkyloxy-, C^alkyloxy- or C1-4alkyloxy substituted with one or where possible two or more substituents selected from Het2, NR7R8, hydroxy and C1-4alkyloxy-Ci-4alkyloxy-; in particular R4 represents Ar4-C1-4alkyloxy-, C1-4alkyloxy- or Ci-4alkyloxy substituted with one or where possible two or more substituents selected from Het2, NR7R8 or hydroxy;
R7 represents hydrogen, hydroxy-C1-4alkyl- or C1-4alkyl;
R8 represents Ci-4alkylcarbonyl , C1-4alkyloxycarbonyl or C^alkyl substituted with hydroxy-C1-4alkyloxy-, NR25R26, Ci-4alkylcarbonyloxy- or C1-4alkylsulfonyl;
R12 represents hydrogen or C1-4alkyl-; R13 represents Ar6-sulfonyl or d.6alkyloxycarbonyl optionally substituted with phenyl;
R16 and R17 each independently represents hydrogen, d_4alkyl or R16 and R17 taken together with the carbon atom to which they are attached from a C3-6cycloalkyl;
R23 represents C^alkyl optionally substituted with Het25;
R23 may also represent hydrogen when R16 and R17 taken together with the carbon atom to which they are attached form a C3_6cycloalkyl;
R25 and R26 each independently represent hydrogen or C1-4alkylcarbonyl;
R27 and R28 each independently represent hydrogen or C1-4alkylcarbonyl;
Het1 represents 2-bora-l,3-dioxolanyl;
Het2 represents piped dinyl, piperazinyl, morpholinyl, thiomorpholinyl or 1,1 -dioxothiomorpholinyl wherein said Het2 is optionally substituted with
Ci-4alkyloxycarbonyl or NR27R28-Ci-4alkyl; in particular Het2 represents 1,1 -dioxothiomorpholinyl; piperidinyl substituted with Ci-4alkyloxycarbonyl; or piperazinyl substituted with Ci-4alkyloxycarbonyl or NR27R28-Ci-4alkyl-;
Het25 represents morpholinyl; Ar4 represents phenyl;
Ar5 represents phenyl; or
Ar6 represents phenyl optionally substituted with nitro.
A further interesting group of compounds consists of those compounds of formula (I) wherein one or more of the following restrictions apply : Z represents NH; Y represents -C3.9alkyl-,-C1-5alkyl-NR13-Cμ5alkyl-,
-C1-5alkyl-NR14-CO-C1-salkyl-, -d^alkyl-NR^-H^CO-NH-d.salkyl- or
-C^alkyl-NR^-CO-CR'V^NH-;
X1 represents O or -O-C1-2alkyl-; X2 represents a direct bond, C1-2alkyl, -CO-C,.2alkyl or NR12-C1-2alkyl;
R1 represents hydrogen or halo; R2 represents halo, acetylene or Het1
R3 represents hydrogen or cyano; R4 represents Ar4-C1-4alkyloxy-, C1-4alkyloxy- or
C^alkyloxy substituted with one or where possible two or more substituents selected from Het2, NR7R8, hydroxy and C]_4alkyloxy-C1-4alkyloxy-; R7 represents hydrogen or Ci-4alkyl; R8 represents C1-4alkyl substituted with NR25R26 or
C1-4alkylsulfonyl; R12 represents hydrogen or Ci-4alkyl-; R13 represents Ar6-sulfonyl or
Ci-δalkyloxycarbonyl optionally substituted with phenyl; R16 and R17 represents hydrogen, C1-4alkyl or R16 and R17 taken together with the carbon atom to which they are attached from a C3-6cycloalkyl;
R23 represents C1-4alkyl and R23 represents hydrogen when R16 and R17 taken together with the carbon atom to which they are attached from a C3-6cycloalkyl; R25, R26, R27 and R28 each independently represent hydrogen or C]-4alkylcarbonyl; Het1 represents 2-bora-l,3-dioxolanyl; Het2 represents piperidinyl, piperazinyl, morpholinyl, thiomorpholinyl or 1,1-dioxothiomorpholinyl wherein said Het2 is optionally substituted with C1-4alkyloxycarbonyl or NR27R28-C1-4alkyl; Ar4 and Ar5 represents phenyl; Ar6 represents phenyl optionally substituted with nitro.
Other special group of compounds are:
- those compounds of formula (I) wherein -X1- represents -O-;
- those compounds of formula (I) wherein -X1- represents C1-2alkyl;
- those compounds of formula (I) wherein -X1- represents -NR11-, in particular -NH-;
- those compounds of formula (I) wherein -X2- represents -NR12-C1-2alkyl, in particular -N(CH3)-C1-2alkyl-;
- those compounds of formula (I) wherein R1 is fluoro, chloro or bromo;
- those compounds of formula (I) wherein R2 is fluoro, chloro or bromo;
- those compounds of formula (I) wherein R2 is Het1, in particular 2-bora-l,3- dioxolanyl; - those compounds of formula (I) wherein R4 is at position 7 of the structure of formula (I). - those compounds of formula (I) wherein R4 represents Ci-4alkyloxy substituted with hydroxy and one substituent selected from NR7R8 or Het2-;
- those compounds of formula (I) wherein R is hydrogen or methyl and R represents aminocarbonyl-C1-4alkyl-, NR25R26, C1-4alkylsulfonyl-Ci_4alkyl-, C^alkylcarbonyloxy-C^alkyl or Hetn-C1-4alkyl-; in particular those compounds of formula (I) wherein R7 is hydrogen or methyl and R8 represents aminocarbonyl-C1-4alkyl-, NR25R26, C1-4alkylsulfonyl-C1-4alkyl- or Hetu-C1-4alkyl-
- those compounds of formula (I) wherein Het2 represent piperidinyl, 1,1-dioxothiomorpholinyl or piperazinyl and said Het2 is optionally substituted with one or where possible two or more substituents selected from NR39R40, aminocarbonyl, mono- or d^C^alkyOaminocarbonyl or C1-4alkylsulfonyl; in particular those compounds of formula (I) wherein Het2 represent piperidinyl or piperazinyl and said Het2 is optionally substituted with one or where possible two or more substituents selected from NR39R40, aminocarbonyl, mono- or di(C1-4alkyl)aminocarbonyl or C^alkylsulfonyl.
In a further embodiment of the present invention the X2 substituent is at position 2', the R1 substituent represents hydrogen or halo and is at position 4', the R2 substituent represents halo and is at position 5', the R substituent is at position 2 and the R4 substituent at position 7 of the structure of formula (I). Alternatively, the X2 substituent is at position 3', the R1 substituent represents hydrogen or halo and is at position 4', the R2 substituent represents halo and is at position 5', the R3 substituent is at position 2 and the R4 substituent at position 7 of the structure of formula (I).
The compounds of this invention can be prepared by any of several standard synthetic processes commonly used by those skilled in the art of organic chemistry and described for instance in the following references; "Heterocyclic Compounds" - Vol.24 (part4) p 261-304 Fused pyrimidines, Wiley - Interscience ; Chem. Pharm. Bull., VoI 41(2) 362-368 (1993); J.Chem.Soc, Perkin Trans. 1, 2001, 130-137.
As further exemplified in the experimental part of the description, a particular group of compounds are those compounds of formula (I) were -X1- represents -O- hereinafter referred to as the compounds of formula (3). Said compounds are generally prepared starting from the known 6-acetoxy-4-chloro-7-methoxy quinazoline (IF) which can be prepared from commercially available veratric acid and 4-hydroxy-3-methoxy benzoic acid, respectively. Coupling of the latter with suitable substituted anilines (HI') under standard conditions, for example stirred in 2-propanol at an elevated temperature ranging form 40-1000C during 3-12 h, furnish the intermediate compounds (IV) (Scheme 1).
Scheme 1
Figure imgf000029_0001
Figure imgf000029_0002
V = hydrogen or a protective group such as for example, methylcarbonyl, t-butyl, methyl, ethyl, benzyl or trialkylsilyl groups
X 7 , R 1 and R 2 are defined as for the compounds of formula (I)
Deprotection of the intermediates of formula (IV) as described in Protective Groups in Organic Synthesis by T. W. Greene and P. G. M. Wuts, 3rd edition, 1998 followed by ring closure under Mitsunobu conditions give the macrocyclic compounds (1) that are used as starting compounds in the synthesis of the final compounds of the present invention. (Scheme 2 - wherein V is defined as hereinbefore).
Figure imgf000029_0003
V = hydrogen or a protective group such as for example, methylcarbonyl, t-butyl, methyl, ethyl, benzyl or trialkylsilyl groups
9 1 9
X , R and R are defined as for the compounds of formula (I)
In brief, said macrocyclic compounds of formula (1) are demethylated using art known conditions such as for example provided in Schemes 3&4 hereinbelow, followed by an alkylation with an appropriate alcohol, such as for example described in Scheme 5 hereinafter. Quinazoline demethylation. Scheme 3:
Figure imgf000030_0001
2
A stirred suspension of 1 (1 equiv), LiCl (7 equiv.) and Na2S.9H2O (7 equiv) in DMF, was heated under microwave conditions to 140° C until completion (30 minutes). The reaction mixture was allowed to cool to ambient temperature and was then poured onto ice water. The mixture was filtered and the yellow precipitation was re-dissolved in DCM/MeOH (9:1) with some HCOOH and purified over silica gel filter (eluens: DCM/MeOH 9.5/0.5). The pure fractions were collected, evaporated and co-evaporated with toluene to give pure 2 (yield: 70 %).
Ouinazoline demethylation. Scheme 4:
Figure imgf000030_0002
To a stirred suspension of 1 (1 equiv) and KI (10 equiv) in DMA, was added HBr (48 % in H2O) while bubbling N2 through the reaction mixture. The mixture was rapidly heated to 130° C and stirred at this temperature until completion (± 2h). The reaction mixture was allowed to cool to 70° C and poured onto ice/H2O/NH3. The mixture was filtered and the yellow precipitation was re-dissolved in THF/MeOH (2:1), concentrated and co-evaporated with toluene. Crystalization from 2-propanol afford pure 2 (yield : 42 - 78 %). Quinazoline alkylation. Scheme 5
Figure imgf000031_0001
wherein R represents Ar4-Ci.4alkyl-, Ci.4alkyl- or R represents Ci_4alkyl substituted with one or where possible two or more substituents selected from hydroxy, halo, CMalkyloxy-,
Figure imgf000031_0002
NR7R8 or Het2-. Ar4, Her2, R7 and R8 are defined as for the compounds of formula (I) hereinbefore.
To a stirred suspension of 2 (1 equiv), alcohol (8 equiv) and triphenylphosphine (2 equiv) in THF, DIAD (2 equiv) was added dropwise and the mixture was stirred at room temperature for 60 min. The reaction mixture was concentrated under reduced pressure, and the crude product was triturated from acetonitrile to afford pure 3.
For those compounds of formula (3) wherein R1 or R2 represent acetylene the following synthesis scheme (Scheme 6) is generally applied. In brief, the halogenated form of the compounds of formula (3) is acetylated using trimethylsilylacetylene followed by deprotection of the acetylene group to yield the compounds of general formula (5).
Incorporation of acetylene moiety. Scheme 6
Figure imgf000031_0003
Pd(Ph3P)-CI- CuI
O ≡≡-L- , 75 "C
Figure imgf000031_0004
4
wherein R is defined as in Scheme 5 hereinbefore To a stirred solution of 3 (1 equiv) in pyrrolidine was added bis(triphenylphosphine)palladium(II)chloride (20 mol%) followed by CuI (cat). The reaction mixture was heated to 750C and trimethylsilylacetylene (2.5 equiv) was added. The mixture was stirred at this temperature until the reaction was essentially complete and was then filtered through a short pad of celite and concentrated to dryness. The residue was re-dissolved in EtOAc and was partitioned between EtOAc and water. The combined organic layers were concentrated under reduced pressure and the residue was treated with MP-TMT in acetonitrile overnight. It was then filtered, the resin was washed with acetonitrile followed by DCM and the filtrate was concentrated to afford 4.
Compound 4 and aqueous K2CO3 (sat.) in MeOH (1:1) were stirred at room temperature for 1 hour. The reaction mixture was concentrated under reduced pressure, the residue was re-dissolved in DCM and washed with water. The organic phase was separated, dried (MgSO4) and concentrated under vaccuo. The residue was purified either by column chromatography or reverse phase HPLC to afford pure 5.
A particular group of compounds are those compounds of formula (3) wherein R represents Ci_4alkyl substituted with NR7R8 or Het2 wherein said Het2 is attached to the remainder of the molecule through the nitrogen atom. Said compounds of general formula (7) are generally made according to synthesis scheme 7 departing from the intermediate compounds of general formula (2).
Scheme 7
Figure imgf000032_0001
wherein R7 and R8 are defined as for the compounds of formula (I), or R7 and R8 taken together with the nitrogen atom to which they are attached from a heterocycle wherein said heterocycle is defined as Het2 for the compounds of formula (I) hereinbefore. To a stirred suspension of 2 (1 equiv), bromopropyl alcohol (2 equiv) and triphenylphosphine (2 equiv) in THF, DIAD (2 equiv) was added dropwise and the mixture was stirred at room temperature for 60 min. The reaction mixture was concentrated under vaccuo, and the crude product was triturated from acetonitrile to afford pure 6.
To a stirred suspension of 6 (1 equiv), in acetonitrile was added the amine (20 equiv) and the mixture was stirred at room temperature overnight. The reaction mixture was concentrated under vaccuo, and the crude product was triturated from acetonitrile to afford pure 7.
Alternatively to the above, and in particular for those compounds of formula (7) wherein the C1-4alkyl moiety is further substituted with hydroxy-, said compounds are made using a nucleophilic addition reaction departing from the oxirane analog 3' (Scheme 8)
Scheme 8
Figure imgf000033_0001
Wherein R7 and R8 are defined as for the compounds of formula (I), or R7 and R8 taken together with the nitrogen atom to which they are attached from a heterocycle wherein said heterocycle is defined as Het2 for the compounds of formula (I) hereinbefore.
To a stirred suspension of 3' (1 equiv), in 2-propanol was added the amine (20 equiv) and the mixture was stirred at 70° C for 2 hours. The reaction mixture was cooled, and the product crystallized from 2-propanol to afford pure 8.
Where necessary or desired, any one or more of the following further steps in any order may be performed :
(i) removing any remaining protecting group(s); (ii) converting a compound of formula (I) or a protected form thereof into a further compound of formula (I) or a protected form thereof; (iii) converting a compound of formula (I) or a protected form thereof into a N-oxide, a salt, a quaternary amine or a solvate of a compound of formula (I) or a protected form thereof;
(iv) converting a N-oxide, a salt, a quaternary amine or a solvate of a compound of formula (I) or a protected form thereof into a compound of formula (I) or a protected form thereof;
(v) converting a JV-oxide, a salt, a quaternary amine or a solvate of a compound of formula (I) or a protected form thereof into another N-oxide, a pharmaceutically acceptable addition salt a quaternary amine or a solvate of a compound of formula
(I) or a protected form thereof; (vi) where the compound of formula (I) is obtained as a mixture of (R) and (S) enantiomers resolving the mixture to obtain the desired enantiomer.
Compounds of formula (I), N-oxides, addition salts, quaternary amines and stereochemical isomeric forms thereof can be converted into further compounds according to the invention using procedures known in the art.
It will be appreciated by those skilled in the art that in the processes described above the functional groups of intermediate compounds may need to be blocked by protecting groups.
Functional groups, which are desirable to protect, include hydroxy, amino and carboxylic acid. Suitable protecting groups for hydroxy include trialkylsilyl groups
(e.g. tert-butyldimethylsilyl, tert-butyldiphenylsilyl or trimethylsilyl), benzyl and tetrahydropyranyl. Suitable protecting groups for amino include tert-butyloxycarbonyl or benzyloxycarbonyl. Suitable protecting groups for carboxylic acid include C(i-6)alkyl or benzyl esters.
The protection and deprotection of functional groups may take place before or after a reaction step.
Additionally, the Ν-atoms in compounds of formula (I) can be methylated by art- known methods using CH3-I in a suitable solvent such as, for example 2-propanone, tetrahydrofuran or dimethylformamide. The compounds of formula (I) can also be converted into each other following art- known procedures of functional group transformation of which some examples are mentioned hereinafter.
The compounds of formula (I) may also be converted to the corresponding TV-oxide forms following art-known procedures for converting a trivalent nitrogen into its TV-oxide form. Said TV-oxidation reaction may generally be carried out by reacting the starting material of formula (I) with 3-phenyl-2-(phenylsulfonyl)oxaziridine or with an appropriate organic or inorganic peroxide. Appropriate inorganic peroxides comprise, for example, hydrogen peroxide, alkali metal or earth alkaline metal peroxides, e.g. sodium peroxide, potassium peroxide; appropriate organic peroxides may comprise peroxy acids such as, for example, benzenecarboperoxoic acid or halo substituted benzenecarboperoxoic acid, e.g. 3-chlorobenzenecarboperoxoic acid, peroxoalkanoic acids, e.g. peroxoacetic acid, alkylhydroperoxides, e.g. t-butyl hydroperoxide. Suitable solvents are, for example, water, lower alkanols, e.g. ethanol and the like, hydrocarbons, e.g. toluene, ketones, e.g. 2-butanone, halogenated hydrocarbons, e.g. dichloromethane, and mixtures of such solvents.
Pure stereochemically isomeric forms of the compounds of formula (I) may be obtained by the application of art-known procedures. Diastereomers may be separated by physical methods such as fractional crystallization and chromatographic techniques, e.g. counter-current distribution, liquid chromatography and the like.
Some of the compounds of formula (I) and some of the intermediates in the present invention may contain an asymmetric carbon atom. Pure stereochemically isomeric forms of said compounds and said intermediates can be obtained by the application of art-known procedures. For example, diastereoisomers can be separated by physical methods such as fractional crystallization or chromatographic techniques, e.g. counter current distribution, liquid chromatography and the like methods. Enantiomers can be obtained from racemic mixtures by first converting said racemic mixtures with suitable resolving agents such as, for example, chiral acids, to mixtures of diastereomeric salts or compounds; then physically separating said mixtures of diastereomeric salts or compounds by, for example, fractional rystallization or chromatographic techniques, e.g. liquid chromatography and the like methods; and finally converting said separated diastereomeric salts or compounds into the corresponding enantiomers. Pure stereochemically isomeric forms may also be obtained from the pure stereochemically isomeric forms of the appropriate intermediates and starting materials, provided that the intervening reactions occur stereospecifically.
An alternative manner of separating the enantiomeric forms of the compounds of formula (I) and intermediates involves liquid chromatography, in particular liquid chromatography using a chiral stationary phase.
Some of the intermediates and starting materials as used in the reaction procedures mentioned hereinabove are known compounds and may be commercially available or may be prepared according to art-known procedures.
As described in the experimental part hereinafter, the growth inhibitory effect and anti- tumour activity of the present compounds has been demonstrated in vitro, in enzymatic assays on the receptor tyrosine kinases such as for example EGFR, AbI, Fyn, FlTl, HcK or the Sar kinase family such as for example Lyn, Yes and cSRC. In an alternative assay, the growth inhibitory effect of the compounds was tested on a number of carcinamo cell lines, in particular in the ovarian carcinoma cell line SKO V3 and the squamous carcinoma cell line A431 using art known cytotoxicity assays such as MTT.
Accordingly, the present invention provides the compounds of formula (I) and their pharmaceutically acceptable JV-oxides, addition salts, quaternary amines and stereochemically isomeric forms for use in therapy. More particular in the treatment or prevention of cell proliferation mediated diseases. The compounds of formula (I) and their pharmaceutically acceptable iV-oxides, addition salts, quaternary amines and the stereochemically isomeric forms may hereinafter be referred to as compounds according to the invention.
Disorders for which the compounds according to the invention are particularly useful are atherosclerosis, restenosis, cancer and diabetic complications e.g. retinopathy.
In view of the utility of the compounds according to the invention, a method of treating a cell proliferative disorder such as atherosclerosis, restenosis and cancer is provided, the method comprising administering to an animal in need of such treatment, for example, a mammal including humans, suffering from a cell proliferative disorder, a therapeutically effective amount of a compound according to the present invention. Said method comprising the systemic or topical administration of an effective amount of a compound according to the invention, to animals, including humans. One skilled in the art will recognize that a therapeutically effective amount of the EGFR inhibitors of the present invention is the amount sufficient to induce the growth inhibitory effect and that this amount varies inter alia, depending on the size, the type of the neoplasia, the concentration of the compound in the therapeutic formulation, and the condition of the patient. Generally, an amount of EGFR inhibitor to be administered as a therapeutic agent for treating cell proliferative disorder such as atherosclerosis, restenosis and cancer, will be determined on a case by case by an attending physician.
Generally, a suitable dose is one that results in a concentration of the EGFR inhibitor at the treatment site in the range of 0.5 nM to 200 μM, and more usually 5 nM to 10 μM. To obtain these treatment concentrations, a patient in need of treatment likely will be administered between 0.01 mg/kg to 300 mg/kg body weight, in particular from 10 mg/kg to 100 mg/kg body weight. As noted above, the above amounts may vary on a case-by-case basis. In these methods of treatment the compounds according to the invention are preferably formulated prior to admission. As described herein below, suitable pharmaceutical formulations are prepared by known procedures using well known and readily available ingredients.
Due to their high degree of selectivity as EGFR inhibitors, the compounds of formula (I) as defined above, are also useful to mark or identify the kinase domain within the receptor tyrosine kinase receptors. To this purpose, the compounds of the present invention can be labelled, in particular by replacing, partially or completely, one or more atoms in the molecule by their radioactive isotopes. Examples of interesting labelled compounds are those compounds having at least one halo which is a radioactive isotope of iodine, bromine or fluorine; or those compounds having at least one 1 lC-atom or tritium atom.
One particular group consists of those compounds of formula (I) wherein R* is a radioactive halogen atom. In principle, any compound of formula (I) containing a halogen atom is prone for radiolabelling by replacing the halogen atom by a suitable isotope. Suitable halogen radioisotopes to this purpose are radioactive iodides, e.g. 122I, 123I, 125I, 131I; radioactive bromides, e.g. 75Br, 76Br, 77Br and 82Br, and radioactive fluorides, e.g. 18F. The introduction of a radioactive halogen atom can be performed by a suitable exchange reaction or by using any one of the procedures as described hereinabove to prepare halogen derivatives of formula (I). Another interesting form of radiolabelling is by substituting a carbon atom by a πC-atom or the substitution of a hydrogen atom by a tritium atom.
Hence, said radiolabeled compounds of formula (I) can be used in a process of specifically marking receptor sites in biological material. Said process comprises the steps of (a) radiolabelling a compound of formula (I), (b) administering this radiolabeled compound to biological material and subsequently (c) detecting the emissions from the radiolabeled compound.
Alternatively the compounds are labeled with stable isotopes. In this form of labeling the naturally abundant isotopes of hydrogen, carbon and nitrogen (1H, 12C and 14N) are replaced with stable isotopes of these elements (2H [deuterium], 13C and 15N, respectively). Labeling with stable isotopes is used for two principal purposes:
Incorporation of stable isotopes into proteins, carbohydrates and nucleic acids facilitates their structural determination at the atomic level. Metabolic studies exploiting the increased mass of compounds labeled with stable isotopes
The term biological material is meant to comprise every kind of material which has a biological origin. More in particular this term refers to tissue samples, plasma or body fluids but also to animals, specially warm-blooded animals, or parts of animals such as organs. When used in in vivo assays, the radiolabeled compounds are administered in an appropriate composition to an animal and the location of said radiolabeled compounds is detected using imaging techniques, such as, for instance, Single Photon Emission Computerized Tomography (SPECT) or Positron Emission Tomography (PET) and the like. In this manner the distribution to the particular receptor sites throughout the body can be detected and organs containing said receptor sites can be visualized by the imaging techniques mentioned hereinabove. This process of imaging an organ by administering a radiolabeled compound of formula (I) and detecting the emissions from the radioactive compound also constitutes a part of the present invention.
In yet a further aspect, the present invention provides the use of the compounds according to the invention in the manufacture of a medicament for treating any of the aforementioned cell proliferative disorders or indications.
The amount of a compound according to the present invention, also referred to here as the active ingredient, which is required to achieve a therapeutical effect will, of course, vary with the particular compound, the route of administration, the age and condition of the recipient, and the particular disorder or disease being treated. A suitable daily dose would be from 0.01 mg/kg to 300 mg/kg body weight, in particular from 10 mg/kg to 100 mg/kg body weight. A method of treatment may also include administering the active ingredient on a regimen of between one and four intakes per day.
While it is possible for the active ingredient to be administered alone, it is preferable to present it as a pharmaceutical composition. Accordingly, the present invention further provides a pharmaceutical composition comprising a compound according to the present invention, together with a pharmaceutically acceptable carrier or diluent. The carrier or diluent must be "acceptable" in the sense of being compatible with the other ingredients of the composition and not deleterious to the recipients thereof.
The pharmaceutical compositions of this invention may be prepared by any methods well known in the art of pharmacy, for example, using methods such as those described in Gennaro et al. Remington's Pharmaceutical Sciences (18th ed., Mack Publishing Company, 1990, see especially Part 8 : Pharmaceutical preparations and their
Manufacture). A therapeutically effective amount of the particular compound, in base form or addition salt form, as the active ingredient is combined in intimate admixture with a pharmaceutically acceptable carrier, which may take a wide variety of forms depending on the form of preparation desired for administration. These pharmaceutical compositions are desirably in unitary dosage form suitable, preferably, for systemic administration such as oral, percutaneous or parenteral administration; or topical administration such as via inhalation, a nose spray, eye drops or via a cream, gel, shampoo or the like. For example, in preparing the compositions in oral dosage form, any of the usual pharmaceutical media may be employed, such as, for example, water, glycols, oils, alcohols and the like in the case of oral liquid preparations such as suspensions, syrups, elixirs and solutions: or solid carriers such as starches, sugars, kaolin, lubricants, binders, disintegrating agents and the like in the case of powders, pills, capsules and tablets. Because of their ease in administration, tablets and capsules represent the most advantageous oral dosage unit form, in which case solid pharma- ceutical carriers are obviously employed. For parenteral compositions, the carrier will usually comprise sterile water, at least in large part, though other ingredients, for example, to aid solubility, may be included. Injectable solutions, for example, may be prepared in which the carrier comprises saline solution, glucose solution or a mixture of saline and glucose solution. Injectable suspensions may also be prepared in which case appropriate liquid carriers, suspending agents and the like may be employed. In the compositions suitable for percutaneous administration, the carrier optionally comprises a penetration enhancing agent and/or a suitable wetting agent, optionally combined with suitable additives of any nature in minor proportions, which additives do not cause any significant deleterious effects on the skin. Said additives may facilitate the administration to the skin and/or may be helpful for preparing the desired compositions. These compositions may be administered in various ways, e.g., as a transdermal patch, as a spot-on or as an ointment.
It is especially advantageous to formulate the aforementioned pharmaceutical compositions in dosage unit form for ease of administration and uniformity of dosage. Dosage unit form as used in the specification and claims herein refers to physically discrete units suitable as unitary dosages, each unit containing a predetermined quantity of active ingredient calculated to produce the desired therapeutic effect in association with the required pharmaceutical carrier. Examples of such dosage unit forms are tablets (including scored or coated tablets), capsules, pills, powder packets, wafers, injectable solutions or suspensions, teaspoonfuls, tablespoonfuls and the like, and segregated multiples thereof.
Experimental part
Hereinafter, the term 'THF' means tetrahydrofuran, 'DIPE' means diisopropyl ether, 'DMF' means N,N-dimethylformamide, 'NaBH(OAc)3' means sodium triacetoxyborohydride, 'EtOAc' means ethyl acetate, 'EDCF means JV- (ethylcarbonimidoyl)-N,N-dimethyl- 1 ,3-propanediamine monohydrochloride, 'HOBT' means 1 -hydroxy- lH-benzotriazole, 'CDF means l,l'-carbonylbis-lH-imidazole, 'DIPEA' means 7V-ethyl-N-(l -methyl ethyl)- 2-propanamine, 'NaBH4' means sodium tetrahydroborate(-l), 'DMA' means dimethylacetamide, 'DIAD' means bis(l- methylethyl) ester diazenedicarboxylic acid, 'HBTU' means 1- [bis(dimethylamino)methylene]-lH-Benzotriazoliumhexafluorophosphate(l-)3-oxide, 'HATU' means l-[bis(dimethylamino)methylene]-lH-l,2,3-triazolo[4,5-b]pyridinium 3-oxide, hexafluorophosphate(l-), 'HOAT' means 3-hydroxy-3H-l,2,3-triazolo[4,5- b]pyridine A. Preparation of the intermediates
Example Al a) Preparation of intermedi
Figure imgf000041_0001
A mixture of N-[(4-chloro-2-nitrophenyl)acetyl]glycine ethyl ester (0.023 mol) in THF (250ml) was hydrogenated with Pt/C (2.0 g) as a catalyst in the presence of a 4% thiophene solution in DIPE (ImI). After uptake of H2 (3 equiv.), the catalyst was filtered off and the filtrate was evaporated. The obtained residue was suspended in DIPE, then the suspension was stirred at boiling temperature, cooled and the desired product was collected by filtration, yielding 6.2g (100%) of intermediate (1). b) Preparation of intermediate (2)
Figure imgf000041_0002
A mixture of 4-chloro-7-methoxy-6-quinazolinol acetate ester (0.00050 mol) and intermediate (1) (0.00050 mol) in 2-propanol (5ml) was stirred for 16 hours in a pressure tube at 800C (oil bath temperature), then the reaction mixture was filtered and the filter residue was air-dried, yielding 0.165g (67.7%) of intermediate (2). c) Preparation of intermediate (3)
Figure imgf000041_0003
A mixture of intermediate (2) (0.0244 mol) in NH3/CH3OH (7N) (50ml) and CH3OH (100ml) was stirred overnight at room temperature and then the solvent was evaporated (Genevac.) under reduced pressure and at room temperature. Finally, the obtained residue was dried (vac.) overnight at 600C, yielding 8.2g (75%) of intermediate (3). d) Preparation of intermediate (4)
Figure imgf000042_0001
A mixture of intermediate (3) (0.0138 mol) and Cs2CO3 (0.0690 mol) in DMF (120ml) was stirred for 30 minutes at room temperature, then 1,2-dibromoethane (0.117 mol) was added and the reaction mixture was stirred overnight at room temperature. The solvent was evaporated under reduced pressure and the residue was co-evaporated with toluene. The obtained residue was stirred in DIPE and the desired product was filtered off, yielding 6.93g (91%) of intermediate (4). e) Preparation of intermediate (5)
Figure imgf000042_0002
A mixture of intermediate (4) (0.00181 mol) and 4-morpholineethanamine (0.00907 mol) in ethanol (20ml) was heated in a microwave oven for 90 minutes at 100°C and then the reaction mixture was purified by reversed-phase high-performance liquid chromatography. The product fractions were collected and the solvent was evaporated, yielding 0.39g (36%) of intermediate (5). f) Preparation of intermediate (6)
Figure imgf000042_0003
A mixture of intermediate (5) (0.00065 mol) and lithium hydroxide (0.0032 mol) in ethanol (20ml) and H2O (2ml) was stirred for 2 hours at room temperature and then the solvent was evaporated under reduced pressure, yielding intermediate (6) (quantitative yield). Example A2 a) Preparation of intermediate (7)
A mixture of 4-chloro-5-fluoro-2-nitrobenzaldehyde (0.0491 mol), N-methyl-L-alanine methyl ester hydrochloride (0.0589 mol) and titanium(4+) 2-propanol salt (0.0737 mol) in 1 ,2-dichloroethane (100ml) was stirred at room temperature for 30 minutes. NaBH(OAc)3 (0.0589 mol) was added. The mixture was stirred overnight, then diluted in CH2Cl2, quenched with aqueous (10%) K2CO3 and filtered. The organic layer was separated, dried (MgSO4), filtered, and the solvent was evaporated to dryness, yielding 16.5g (quantitative yield) of intermediate (7) (S-configuration). b) Preparation of intermediate (8)
Figure imgf000043_0002
A mixture of intermediate (7) (0.0491 mol), Fe (0.246 mol) and NH4Cl (0.491 mol) in THF/CH3OH/H2O (4/4/2; 500ml) was stirred and refluxed overnight, then cooled to room temperature and filtered. The filtrate was diluted in CH2Cl2. The organic layer was separated, dried (MgSO4), filtered and the solvent was evaporated to dryness, yielding 13g (96%) of intermediate (8) (S-configuration). c) Preparation of intermediate (9)
Figure imgf000043_0003
A mixture of 4-chloro-7-methoxy-6-quinazolinol acetate ester (0.0162 mol) and intermediate (8) (0.0162 mol) in CH3CN (150ml) was stirred and refluxed for 4 hours, then cooled back to room temperature, the solvent was evaporated in vacuo and the residue was taken up in K2CO3 (aq.) (10%) and CH2Cl2. The organic layer was separated, dried (MgSO4), filtered, and the solvent was evaporated to dryness. The residue (6.4g) was purified by column chromatography over silica gel (eluent: CH2C12/CH3OH 100/0 to 99/1 ; 15-40μm). The desired fractions were collected and the solvent was evaporated, yielding 3.09g (37%) of intermediate (9) (S-configuration). d) Preparation of intermediate (10)
Figure imgf000044_0001
A mixture of intermediate (9) (0.0061 mol) in NH3/CH3OH (7N) (20ml) and CH3OH (100ml) was stirred at room temperature for 40 hours, then evaporated to dryness. The residue was taken up in CH3CN/DIPE. The precipitate was filtered off and dried, yielding 1.93g (70%) of intermediate (10) (M.P.: 234°C; S-configuration). e) Preparation of intermediate (11)
Figure imgf000044_0002
Cs2CO3 (0.0063 mol) was added to a solution of intermediate (10) (0.0042 mol) in dry DMF (20ml). The mixture was stirred at room temperature for 1 hour. A solution of (3- bromopropyl)-l,l-dimethylethyl ester carbamic acid (0.0046 mol) in dry DMF (5ml) was added. The mixture was stirred at room temperature for 3 hours, poured into H2O and extracted with EtOAc. The organic layer was separated, dried (MgSO4), filtered, and the solvent was evaporated to dryness, yielding: 2.8g (quantitative yield) of intermediate (11) (S-configuration). f) Preparation of intermediate (12)
Figure imgf000044_0003
A mixture of intermediate (11) (0.0042 mol) in HCl (aq.) (6N) (20ml) and dioxane (100ml) was stirred at 600C for 3 hours, then cooled to room temperature and evaporated to dryness. The residue was taken up in ethanol/diethyl ether. The precipitate was filtered under N2 flow and dried in vacuo, yielding 2.24g (100%) of intermediate (12) as a hydrochloric acid salt(.3.02HCl .1.88H2O; S-configuration; M.P. 175°C). g) Preparation of intermediate (13)
Figure imgf000045_0001
Intermediate (12) (0.0018 mol) was added portionwise to a warm solution (50°C) of EDCI (0.0037 mol), HOBT (0.0037 mol) and triethylamine (0.008 mol) in CH2C12/THF (50/50; 1000ml) over a 3 hour period, under vigorous stirring at 50°C. After evaporation of the solvent, the residue was taken up in K2CO3 (aq.) (10%). The mixture was extracted with CH2Cl2. The organic layer was separated, dried (MgSO4), filtered and the solvent was evaporated to dryness. The residue (Ig) was crystallized from ethanol/DIPE. The precipitate was filtered off and dried. This fraction was crystallized again from CH3CN. The precipitate was filtered off and dried, yielding 0.2 Ig (24%) of intermediate (13) (M.P.: 270°C; S-configuration). h) Preparation of intermediate (14)
Figure imgf000045_0002
A mixture of intermediate (13) (0.0001 mol), sodium sulfide (0.001 mol) and lithium chloride (0.0011 mol) in DMF (ImI) was stirred at room temperature for 5 minutes, then heated in a microwave oven at 90°C for 15 minutes, poured into saturated NaHCO3 and extracted with diethyl ether three times. The organic layer was washed with saturated NaCl, dried (MgSO4), filtered and the solvent was evaporated to dryness. The residue (1.3g) was purified by column chromatography over silica gel (eluent: CH2C12/CH3OH 100/0 to 90/10; l5-40μm). The desired fractions were collected and the solvent was evaporated. The residue was crystallized from CH3CN. The precipitate was filtered off and dried, yielding 0.406g (84%) of intermediate (14) (M.P.: 196°C; S- configuration). i) Preparation of intermediate (15)
Figure imgf000045_0003
l-Bromo-3-chloropropane (0.0012 mol) was added to a suspension of intermediate (14) (0.0008 mol) and K2CO3 (aq.) (0.0016 mol) in CH3CN/DMF (8ml). The mixture was stirred and refluxed for 18 hours, then cooled to room temperature, poured into H2O and extracted with EtOAc. The organic layer was separated, dried (MgSO4), filtered and the solvent was evaporated to dryness. The residue (0.85g) was purified by column chromatography over silica gel (eluent: CH2C12/CH3OH 100/0 to 97/3; 15-40μm). The pure fractions were collected and the solvent was evaporated, yielding 0.24g (58%) of intermediate (15) (S-configuration).
Example A3 a) Preparation of intermediate (16)
Figure imgf000046_0001
A mixture of intermediate (15) (0.0005 mol), 1,1-dimethylethyl ester 1- piperazinecarboxylic acid (0.001 mol) and K2CO3 (aq.) (0.0005 mol) in CH3CN (3ml) was stirred and refluxed overnight. 1,1-Dimethylethyl ester 1-piperazinecarboxylic acid (0.001 mol) and K2CO3 (aq.) (0.0005 mol) were added again. The mixture was stirred and refluxed for 18 hours, cooled to room temperature, poured into H2O and extracted with CH2Cl2. The organic layer was separated, dried (MgSO4), filtered and the solvent was evaporated to dryness. The residue (0.487g) was purified by column chromatography over kromasil (eluent: CH2CI2ZCH3OHZNH4OH 99Z1Z0.05 to 90Z10/0.5; 5μm). The pure fractions were collected and the solvent was evaporated, yielding 0.165g (46%) of intermediate (16) (S-configuration; M.P.: 140°C). b) Preparation of intermediate (17)
Figure imgf000046_0002
HCl/2-propanol (0.3ml) was added to a mixture of intermediate (16) (0.0001 mol) in CH3OH (3ml). The mixture was stirred at room temperature overnight, then stirred at room temperature for 18 extra hours and evaporated to dryness. This hydrochloric acid salt was taken up in K2CO3 (aq.) (10%). The mixture was extracted with CH2Cl2. The organic layer was separated, dried (MgSO4), filtered and the solvent was evaporated to dryness, yielding: 0.095g (100%) of intermediate (17) (S-configuration). Example A4 a) Preparation of intermediate (18)
Figure imgf000047_0001
A mixture of 4-bromo-2-nitrobenzeneacetic acid (0.077 mol) and HOBT (0.077 mol) in CH2Cl2 (550ml) was stirred at room temperature. CDI (0.077 mol) was added and stirring was continued for 10 minutes. Then DIPEA (0.077 mol) was added and the reaction mixture was stirred at room temperature for 30 minutes. L-Leucine methyl ester hydrochloride (0.077 mol) was added at once and the mixture was stirred overnight at room temperature. An extra amount of HOBT (0.077 mol), CDI (0.077 mol) and DIPEA (0.077 mol) was added and the reaction mixture was stirred at room temperature over the weekend. The mixture was quenched with H2O and the layers were separated. The organic layer was washed with saturated K2CO3 (aq.) (Ix) and HCl (IN) (Ix), then dried (MgSO4), filtered and the solvent was evaporated. The red gum- like product was triturated from 2-propanol. The off-white solid was filtered off and dried, yielding 7.87g of intermediate (18) (S-configuration). b) Preparation of intermediate (19)
Figure imgf000047_0002
A mixture of intermediate (18) (0.056 mol) in toluene (219ml) was stirred (mixture (I)). A mixture Of NH4Cl (0.283 mol) in H2O (151ml) was added to mixture (1) and in a next step Fe (0.283 mol) was added. The reaction mixture was refluxed overnight. Then another portion of NH4Cl (0.283 mol) and Fe (0.283 mol) was added and the reaction mixture was refluxed for 1 hour. The mixture was cooled to room temperature and then filtered through dicalite. The layers were separated and the aqueous layer was washed with toluene. The combined organic layers were dried (MgSO4), filtered and the solvent was evaporated, yielding 20. Ig of intermediate (19) (S-configuration). c) Preparation of intermediate (20)
Figure imgf000047_0003
A solution of intermediate (19) (0.055 mol) in 2-propanol (200ml) was heated to 70°C (solution (I)). A solution of 4-chloro-7-methoxy-6-quinazolinol acetate ester (0.066 mol) in 2-propanol (200ml) was also heated to 7O0C and this solution was added to solution (1). Stirring at 7O0C was continued for 75 minutes. An extra amount of 4- chloro-7-methoxy-6-quinazolinol acetate ester (0.027 mol) in 2-ρropanol (100ml) was added and the mixture was reacted further for 2 hours. The solvent was evaporated and the residue was purified by column chromatography over silica gel (eluent: CH2C12/CH3OH 99.5/0.5 till 90/10). The product fractions were collected and the solvent was evaporated, yielding 13.82g of intermediate (20) (S-configuration). d) Preparation of intermediate (21)
Figure imgf000048_0001
A mixture of intermediate (20) (0.081 mol) in CH3OH (400ml) was stirred at room temperature. NH3/CH3OH (7N) (200ml) was added and the reaction mixture was stirred at room temperature for 95 minutes. The solvent was evaporated and the residue was triturated from 2-propanol. The pale yellow solid was filtered off and dried, yielding 43g (99.9%) of intermediate (21) (S-configuration). e) Preparation of intermediate (22)
Figure imgf000048_0002
A mixture of intermediate (21) (0.0113 mol) in DMF (300ml) was stirred. K2CO3 (aq.) (0.056 mol) was added and the reaction mixture was stirred at room temperature for 35 minutes. Then 1,3-dibromopropane (0.113 mol) was added and the reaction mixture was stirred for 40 hours at room temperature. The reaction mixture was filtered and concentrated under reduced pressure till ~20ml. The concentrate was poured into H2O and the precipitation was filtered off and dried, yielding 7.16g (97.1%) of intermediate (22) (S-configuration). f) Preparation of intermediate (23)
Figure imgf000049_0001
A mixture of intermediate (22) (0.003832 mol) and 4-morpholinepropanamine (0.0383 mol) in ethanol (40ml) was heated to 1000C for 1 hour and then purified by high performance liquid chromatography. The organic solvent was evaporated and the water layer was concentrated to ~20ml. The concentrate was made alkaline with aqueous NaOH (IN) to a pH of -10 and extracted with EtOAc. The separated organic layer was dried (MgSO4), filtered and the mixture was concentrated, yielding 9.14g of intermediate (23) (S-configuration). g) Preparation of intermediate (24)
Figure imgf000049_0002
A mixture of intermediate (23) (0.007909 mol) in CH3OH (40ml) and H2O (4ml) was stirred at room temperature until dissolution. Lithium hydroxide (0.0395 mol) was added and the reaction mixture was stirred for 85 minutes. The reaction mixture was concentrated and the residue was dried, yielding 5.53 g (99.6%) of intermediate (24) (S-configuration). h) Preparation of intermediate (25)
Figure imgf000049_0003
A mixture of HATU (0.002052 mol) and HOAT (0.00008551 mol) in DMA (50ml) was added dropwise to a mixture of intermediate (24) (0.0007126 mol) and DIPEA (0.002138 mol) in DMA (50ml). The reaction mixture was stirred overnight. H2O was added and the mixture was concentrated to -10ml. EtOAc was added to the mixture to become a solution. H2O was added and the two layers were separated. The organic layer was dried (MgSO4), filtered and the solvent was evaporated. The obtained residue was purified by high performance liquid chromatography (NH4HCO3 buffer). The product fractions were collected, the solvent was evaporated and the residue was dried, yielding intermediate (25) (S -configuration; quantitative yield), i) Preparation of intermediate (26)
Figure imgf000050_0001
A mixture of intermediate (25) (0.0007314 mol) in pyrrolidine (10ml) was stirred dichlorobis(triphenylphosphine)palladium (0.00003657 mol) and copper iodide (catalytic amount) were added and the reaction mixture was heated to 75°C.
Ethynyltrimethylsilane (0.001828 mol) was added and heating was continued for 30 minutes. Then an extra portion of dichlorobis(triphenylphosphine)palladium (0.00003657 mol) and ethynyltrimethylsilane (0.001828 mol) was added and the mixture was reacted for 270 minutes. The reaction mixture was filtered through celite and washed with CH3OH. The solvent was evaporated and the residue was redissolved in EtOAc and washed 2x with H2O. The organic layer was dried (MgSO4), filtered and the solvent was evaporated. The crude residue was redissolved in CH3CN and MP- TMT resin (0.0003657 mol) was added to scavenge any residual Pd. This mixture was stirred for 36 hours at room temperature and was then filtered. The resin was washed with CH3OH and the filtrate was evaporated, yielding 0.48 g of intermediate (26) (S- configuration).
Example A5 a) Preparation of intermediate (27)
Figure imgf000050_0002
4-Chloro-l-(chloromethyl)-2-nitrobenzene (0.81 mol) and propanedioic acid diethyl ester (0.794 mol) were suspended in hexane (300ml). K2CO3 (aq.) (0.81 mol) was added. Then, 18-crown-6 (0.008 mol) was added. The resultant reaction mixture was stirred and refluxed for 30 hours under N2 atmosphere. The reaction mixture was cooled to 20°C. This mixture was extracted with water (750ml). The layers were separated. The aqueous phase was washed with toluene. The combined organic layers were dried (Na2SO4), filtered and the solvent was evaporated, yielding 255.8g of intermediate (27). b) Preparation of intermediate (28)
Figure imgf000051_0001
Intermediate (27) (255.8g, 0.466 mol) was dissolved in acetic acid (1000ml). A 20% aqueous HCl solution (1000ml) was added and the resulting reaction mixture was stirred and refluxed for 16 hours. The reaction mixture was cooled to 20°C and the solvent was evaporated. The residue was suspended in water (500ml) and treated with a 10% aqueous NaOH solution (500ml). This mixture was stirred for one hour. This mixture was extracted with diethyl ether (3 x 500ml) and then acidified with concentrated HCl resulting in precipitation from the cooled aqueous layer. The precipitate was filtered off and dried, yielding 109g of intermediate (28) (M.P.: 109- Hl0C). c) Preparation of intermediate (29)
Figure imgf000051_0002
A mixture of intermediate (28) (0.015 mol) and HOBT (0.015 mol) in CH2Cl2 (10ml) was stirred for 30 minutes at room temperature. CDI (0.015 mol) was added and the reaction mixture was stirred for 30 minutes at room temperature. The resultant solution was added to a mixture of α-aminocyclohexanepropanoic acid methyl ester hydrochloride (0.01875 mol) and diisopropylmethylamine/resin (1 0.05 mol) in CH2Cl2 (70ml) and the reaction mixture was shaken overnight at room temperature. An excess of scavenger resins (polystyrylmethyl)trimethylammonium bicarbonate and sulfonic acid resin MP (70-90 mesh) were added and the mixture was shaken for 18 hours. The mixture was filtered. The filtrate was concentrated at room temperature, yielding intermediate (29) (S-configuration; quantitative yield), d) Preparation of intermediate (30)
Figure imgf000051_0003
A mixture of intermediate (29) (0.001 mol) in 2-propanol (20ml) was hydrogenated with 5% Pt/C (catalytic quantity) as a catalyst in the presence of vanadium oxide (q.s.) and a 4% thiophene solution in DIPE (q.s.). After uptake of H2 (3 equiv), the catalyst was filtered off and the filtrate was evaporated, yielding intermediate (30) (S- configuration; quantitative yield). e) Preparation of intermediate (31)
Figure imgf000052_0001
A mixture of 4-chloro-7-methoxy-6-quinazolinol acetate ester (0.001 mol) and intermediate (30) (1 equiv; 0.001 mol) in 2-propanol (25ml) was stirred for 6 hours at 800C. The reaction mixture was cooled to room temperature and used as such in next reaction step, yielding intermediate (31) (S-configuration; quantitative yield), f) Preparation of intermediate (32)
Figure imgf000052_0002
A mixture of intermediate (31) (0.0010 mol) in 2-propanol (25ml) and NH3/CH3OH (5ml) was stirred for 18 hours at room temperature. The solvent was evaporated under reduced pressure. The residue was purified by high-performance liquid chromatography. The product fractions were collected and the solvent was evaporated, yielding intermediate (32) (S-configuration; quantitative yield), g) Preparation of intermediate (33)
Figure imgf000052_0003
A mixture of intermediate (32) (crude) and Cs2CO3 (5 equiv.) in DMF (5ml) was stirred for 30 minutes at room temperature. (3-bromopropyl)-l,l-dimethylethyl ester carbamic acid (1.1 equiv.) was added and the reaction mixture was stirred for 18 hours at room temperature. The solvent was evaporated under reduced pressure, yielding intermediate (33) (S-configuration; quantitative yield). h) Preparation of intermediate (34)
Figure imgf000053_0001
A solution of intermediate (33) (crude) in HCl (6N) (2ml) and dioxane (2ml) was stirred for 16 hours at 60°C. The solvent was evaporated under reduced pressure, yielding of intermediate (34) (S-configuration; quantitative yield).
Example A6 a) Preparation of intermediate (35)
Figure imgf000053_0002
A solution of 4-bromo-2-nitrobenzaldehyde (0.013 mol), 5-amino-l-pentanol (0.013 mol) and titanium(4+) 2-propanol salt (0.014 mol) in ethanol (15ml) was stirred at room temperature for 1 hour, then the reaction mixture was heated to 50°C and stirred for 30 minutes. The mixture was cooled to room temperature and NaBH4 (0.013 mol) was added portionwise. The reaction mixture was stirred overnight and then poured onto ice water (50ml). The resulting mixture was stirred for 20 minutes, the formed precipitate was filtered off (giving Filtrate (I)), washed with H2O and stirred in CH2Cl2 (to dissolve the product and to remove it from the Ti-salt). The mixture was filtered and then the filtrate was dried (MgSO4) and filtered, finally the solvent was evaporated to dryness. Filtrate (I) was evaporated until ethanol was removed and the aqueous concentrate was extracted 2 times with CH2Cl2. The organic layer was separated, dried (MgSO4), filtered off and the solvent was evaporated dry, yielding 3.8 g (93%) of intermediate (35). b) Preparation of intermediate (36)
Figure imgf000053_0003
A solution of intermediate (35) (0.0047 mol), formaldehyde (0.025 mol) and titanium(4+) 2-propanol salt (0.0051 mol) in ethanol (150ml) was heated to 50°C and stirred for 1 hour, then NaBH4 (0.026 mol) was added portionwise at room temperature. The reaction mixture was stirred overnight and then quenched with water (100ml). The resulting mixture was stirred for 1 hour; the formed precipitate was filtered off and washed. The organic filtrate was concentrated, then the aqueous concentrate was extracted with CH2Cl2 and dried. The solvent was evaporated dry and the residue was filtered over silica gel (eluent: CH2C12/CH3OH from 98/2 to 95/5). The product fractions were collected and the solvent was evaporated dry, yielding 0.5g of intermediate (36). c) Preparation of intermediate (37)
Figure imgf000054_0001
A solution of intermediate (36) (0.0015 mol) and pyridine (0.015 mol) in acetic acid anhydride (8ml) was stirred overnight at room temperature, then the solvent was evaporated and co-evaporated with toluene, yielding intermediate (37) (quantitative yield), d) Preparation of intermediate (38)
Figure imgf000054_0002
A mixture of intermediate (37) (0.0015 mol) in THF (50ml) was hydrogenated with 5% Pt/C (0.5 g) as a catalyst in the presence of a 4% thiophene solution in DIPE (0.5ml). After uptake of H2 (3 equiv.), the catalyst was filtered off and the filtrate was evaporated, yielding 0.5g of intermediate (38). e) Preparation of intermediate (39)
Figure imgf000054_0003
A mixture of intermediate (38) (0.0015 mol) and 4-chloro-7-methoxy-6-quinazolinol acetate ester (0.0015 mol) in 2-propanol (30ml) was heated to 80°C and the reaction mixture was stirred for 1 day. The solvent was evaporated under reduced pressure, yielding 0.83g of intermediate (39). f) Preparation of intermediate (40)
Figure imgf000054_0004
A solution of intermediate (39) (0.0015 mol) in CH3OH (25ml) was stirred at room temperature and a solution of K2CO3 (0.003 mol) in H2O (2.5ml) was added, then the reaction mixture was heated to 60°C and stirred for 18 hours. The solvent was evaporated and H2O (20ml) was added, then the mixture was neutralised with acetic acid and the formed precipitate was filtered off. The filtrate was concentrated under reduced pressure and the concentrate was extracted with CH2Cl2, filtered, then dried (MgSO4) and the mixture was concentrated under reduced pressure, yielding 0.5g (70%) of intermediate (40). g) Preparation of intermediate (41)
Figure imgf000055_0001
A solution of intermediate (40) (0.0011 mol) in THF (50ml) was stirred at room temperature and tributylphosphine (0.0016 mol) was added, then 1,1'- (azodicarbonyl)bispiperidine (0.0016 mol) was added and the reaction mixture was stirred for 2 hours. The solvent was evaporated until 1/3 of the initial volume. The resulting precipitate was filtered off and washed. The filtrate was evaporated and the residue was purified by high-performance liquid chromatography. The product fractions were collected and the organic solvent was evaporated. The aqueous concentrate was extracted 2 times with CH2Cl2 and the organic layer was dried (MgSO4). The solvent was evaporated dry and the residue was dried (vacuum) at 50°C, yielding 0.004g (0.8 %) of intermediate (41). h) Preparation of intermediate (42)
Figure imgf000055_0002
A 48% solution of hydrobromide in water (5.5ml) was added to a suspension of intermediate (41) (0.0058 mol) and potassium iodide (0.044 mol) in DMA (55ml), stirred at room temperature under N2 flow. The reaction mixture was stirred for 2.5 hours at 13O0C. The reaction mixture was poured onto ice water. The layers were separated. The aqueous layer was neutralised with NaOH (IN) and the resulting precipitate was filtered off, then dissolved in CH2Cl2, washed with water, separated and the organic phase was dried, filtered and the solvent evaporated under reduced pressure. The residue was stirred in water, filtered off, dissolved in THF and the solvent was evaporated (toluene was added and azeotroped on the rotary evaporator), yielding 1.58g (61%) of intermediate (42). i) Preparation of intermediate (43)
Figure imgf000056_0001
Bis(l-methylethyl) ester diazenedicarboxylic acid (0.0158 mol) was added dropwise to a suspension of intermediate (42) (0.007895 mol), 2-(2-methoxyethoxy)ethanol (0.0631 mol) and triphenylphosphine (0.0158 mol) in THF (120ml), stirred at room temperature. The reaction mixture was stirred at room temperature for 20 minutes. The solvent was evaporated in vacuo. The residue was stirred for 10 minutes in CH3CN at room temperature. The precipitate was filtered off, washed with CH3CN and dried, yielding 3.37g (78%) of intermediate (43). j) Preparation of intermediate (44)
Figure imgf000056_0002
Intermediate (43) (0.0009166 mol) was stirred in pyrrolidine (10ml). Dichlorobis(triphenylphosphine)palladium (0.00004583 mol) was added, followed by addition of copper iodide (catalytic quantity). The mixture was heated to 70°C.
Ethynyltrimethylsilane (0.002292 mol) was added and the reaction mixture was stirred at 70°C for 4.75 hours. The reaction mixture was cooled to room temperature, filtered through dicalite and the filter residue was washed with CH3OH. The filtrate was concentrated. The concentrate was redissolved in EtOAc, then partitioned between water and EtOAc. The organic layer was separated, dried (MgSO4), filtered and the solvent was evaporated. The residue was redissolved in CH3CN and treated with MP- TMT resin (0.002292 mol) to scavenge any residual Pd. The mixture was stirred slowly over the weekend. The mixture was filtered. The resin was washed with CH3OH and the filtrate's solvent was evaporated, yielding 0.40Og of intermediate (44).
Example A7 a) Preparation of intermediate (45)
Figure imgf000056_0003
A solution of 2-(methylamino)ethanol (0.077 mol) in CH2Cl2 (180ml) was stirred at room temperature. Tetrakis(2-methyl-2-propanolato)titanate(l-) (0.077 mol) was added, followed by triethylamine (0.077 mol). 4-Bromo-5-fluoro-2-nitrobenzaldehyde (0.077 mol) was added and the mixture was stirred for 90 minutes. NaBH(OAc)3 (0.0847 mol) was added and the reaction mixture was stirred for 18 hours at room temperature. The mixture was poured into an aqueous NaHCO3 solution. The precipitate was filtered off. The layers were separated. The organic phase was washed with water (2 x), dried (MgSO4), filtered and the solvent was evaporated. The residue was purified by column chromatography over silica gel (eluent: CH2Cl2ZCH3OH 99/1 to 99/2). The desired fractions were collected and the solvent was evaporated, yielding 18g of intermediate (45). b) Preparation of intermediate (46)
Figure imgf000057_0001
A mixture of intermediate (45) (0.059 mol) in EtOAc (250ml) was hydrogenated at room temperature and atmospheric pressure with 5% Pt/C (2 g) as a catalyst in the presence of vanadium oxide (0.5 g) and a 4% thiophene solution in DIPE (2ml). After uptake of H2 (3 equiv), the catalyst was filtered off and the filtrate was evaporated, yielding intermediate (46) (quantitative yield), c) Preparation of intermediate (47)
Figure imgf000057_0002
A mixture of intermediate (46) (0.0396 mol) and 4-chloro-7-methoxy-6-quinazolinol acetate ester (0.0396 mol) in 2-propanol (300ml) was stirred for 1 day at 75°C. More 4- chloro-7-methoxy-6-quinazolinol acetate ester (5 g) was added and the reaction mixture was stirred again for 1 day at 75°C. The solvent was evaporated under reduced pressure, yielding intermediate (47) (quantitative yield), d) Preparation of intermediate (48)
Figure imgf000057_0003
A mixture of intermediate (47) (0.0396 mol) in NH3/CH3OH (200ml) and CH3OH (100ml) was stirred overnight at room temperature. The resulting precipitate was filtered off, washed and dried (vacuum, 600C), yielding 15.7g of intermediate (48). e) Preparation of intermediate (49)
Figure imgf000058_0001
A solution of intermediate (48) (0.0347 mol) in DMF (150ml) was stirred at room temperature and treated with K2CO3 (aq.) (0.16 mol). The reaction mixture was stirred for 45 minutes at room temperature. 1,3-Dibromopropane (0.31 mol) was added and the reaction mixture was stirred for 2 hours at room temperature. The mixture was poured onto ice/water, stirred for 10 minutes, and the resulting precipitate was filtered off, washed and dried (vacuum, 60°C). The solid was stirred in DIPE, filtered off, washed, then dried again in vacuo at 60°C, yielding 19.2g (97%) of intermediate (49). f) Preparation of intermediate (50)
Figure imgf000058_0002
A solution of intermediate (49) (0.033 mol), 2-nitrobenzenesulfonamide (0.10 mol) and triphenylphosphine (0.0495 mol) in THF (700ml) was stirred at room temperature. A solution of bis(l-methylethyl) ester diazenedicarboxylic acid (0.0495 mol) in THF
(50ml) was added dropwise and the reaction mixture was stirred overnight. The solvent was evaporated under reduced pressure. The residue was purified by column chromatography over silica gel. The product fractions were collected and the solvent was evaporated, yielding intermediate (50) (quantitative yield).
Example A8 a) Preparation of intermediate (51)
Figure imgf000058_0003
DIAD (0.005 mol) was added dropwise to a mixture of intermediate (42) (0.0017 mol), (2R)-xiranemethanol (0.0105 mol) and triphenylphosphine (0.005 mol) in THF (30ml), stirred at room temperature for 5 hours. The precipitate was filtered off, washed with THF, and dried, yielding 0.545 g (64%) of intermediate (51) (R-configuration). Example A9 a) Preparation of intermediate (52)
Figure imgf000059_0001
DIAD (0.0003 mol) was added dropwise to a solution of intermediate (42) (0.000138 mol), 3-bromo-l-propanol (0.00055 mol) and triphenylphosphine (0.0003 mol) in THF (2ml), stirred at room temperature. The reaction mixture was stirred for 1 hour at room temperature. The solvent was evaporated under a gentle flow of N2, yielding intermediate (52) (quantitative yield).
B. Preparation of the compounds
Example B 1 Preparation of compound (1)
Figure imgf000059_0002
HBTU (0.00195 mol) was added to a stirred solution of intermediate (6) (0.00069 mol) and DIPEA (0.00324 mol) in N,iV-dimethylacetamide (250ml) at room temperature, then the reaction mixture was stirred for 3 hours and the solvent was co-evaporated with toluene under reduced pressure. The obtained residue was purified by reversed- phase high-performance liquid chromatography (eluent 1: NH4OAc; eluent 2: NH4HCO3). The pure product fractions were collected and the solvent was evaporated under reduced pressure. The obtained residue (0.030 g) was crystallised from 2-propanol, then the resulting precipitate was filtered off and dried (vacuum), yielding 0.0165g of compound (1). Example B2 Preparation of compound (2) and compound (3)
Figure imgf000060_0001
compound (2) compound (3)
A mixture of intermediate (15) (0.0002 mol), 2-(methylamino)ethanol (0.0005 mol) and K2CO3 (aq.) (0.0002 mol) in CH3CN (1.5ml) was stirred and refluxed overnight, then cooled to room temperature, poured into H2O and extracted with CH2Cl2. The organic layer was separated, dried (MgSO4), filtered and the solvent was evaporated to dryness. The residue (0.16g) was purified by column chromatography over kromasil (eluent: CH2Cl2ZCH3OHyNH4OH 99/1/0.05 to 88/12/1.2; 5μm). Two fractions were collected and the solvent was evaporated, yielding 0.009g (6%) of compound (3) (S-configuration) and 0.05g (31%) of compound (2) (S-configuration).
Example B 3 Preparation of compound (4)
Figure imgf000060_0002
A mixture of intermediate (15) (0.0002 mol), iV-(2-aminoethyl)acetamide (0.0005 mol) and K2CO3 (aq.) (0.0002 mol) in CH3CN (1.5ml) was stirred and refluxed overnight. iV-(2-aminoethyl)acetamide and K2CO3 (aq.) were added again. The mixture was stirred and refluxed for 5 hours, then cooled to room temperature, poured into H2O and extracted with CH2Cl2. The organic layer was separated, dried (MgSO4), filtered and the solvent was evaporated to dryness. The residue (0.146g) was purified by column chromatography over kromasil (eluent: CH2CI2ZCH3OHZNH4OH 99/1/0.05 to 75/25/1; 5μm). The pure fractions were collected and the solvent was evaporated. The residue (0.042g, 27%) was crystallized from diethyl ether. The precipitate was filtered off and dried, yielding 0.034g (22%) of compound (4) (S-configuration; M.P.: 112°C). Example B4 Preparation of compound (5)
Figure imgf000061_0001
A mixture of intermediate (15) (0.0002 mol), ethanolamine (0.0005 mol) and K2CO3 (aq.) (0.0002 mol) in CH3CN (1.5ml) was stirred and refluxed overnight. CH3OH was added. The mixture was stirred at room temperature for 18 hours, poured into H2O and extracted with EtOAc. The organic layer was separated, dried (MgSO4), filtered and the solvent was evaporated to dryness. The residue (0.12g) was purified by column chromatography over kromasil (eluent: CH2CI2ZCH3OHZNH4OH 96Z4Z0.4 to 86Z4Z1.4; 5μm). The pure fractions were collected and the solvent was evaporated, yielding 0.048g (33%) of compound (5) (S-configuration).
Example B 5 Preparation of compound (6)
Figure imgf000061_0002
A mixture of intermediate (17) (0.0001 mol), iV-(2-chloroethyl)acetarnide (0.0001 mol), K2CO3 (aq.) (0.0003 mol) and potassium iodide (0.004g) in ethanol (3ml) was stirred and refluxed for 3 days, then cooled to room temperature, poured into H2O and extracted with CH2Cl2. The organic layer was separated, dried (MgSO4), filtered and the solvent was evaporated to dryness. The residue (0.097g) was purified by column chromatography over kromasil (eluent: CH2CI2ZCH3OHZNH4OH 96Z4Z0.5 to 90Z10Z0.5; 5μm). The pure fractions were collected and the solvent was evaporated. The residue (0.042g, 42%) was crystallized from CH3CN. The precipitate was filtered off and dried, yielding 0.032g (32%) of compound (6) (S-configuration; M.P.: 136°C). Example B 6 Preparation of compound (7)
Figure imgf000062_0001
A mixture of intermediate (26) (0.0006848 mol) in a saturated aqueous K2CO3 solution (60ml) and CH3OH (60ml) was stirred for 30 minutes at room temperature. The solvent was evaporated and the residue was dissolved in CH2C12/H2O. The layers were separated and the organic layer was dried (MgSO4), filtered and the solvent was evaporated. The crude residue was purified by flash column chromatography over silica gel (eluent: CH2C12/CH3OH 99/1 till 95/5; column was stripped with CH2C12/(CH3OH/NΗ3) 95/5). The desired fractions were purified again by column chromatography over silica gel (eluent: CH2C12/(CH3OH/NH3) 100/0 to 97/3). The product fractions were collected and the solvent was evaporated. The residue was purified by high-performance liquid chromatography (ammonium acetate buffer). The product fractions were collected, the CH3CN was evaporated and the aqueous layer was made alkaline (pH=10). The product was extracted with CH2Cl2. The separated organic layer was dried and the solvent was evaporated, yielding 0.419 g of compound (7) (S -configuration) .
Example B7 Preparation of compound (8)
Figure imgf000062_0002
A solution of HBTU (excess) and DIPEA (3 equiv) in DMF (3ml) was stirred at room temperature. A solution of intermediate (34) (crude) in DMF (2ml) was added dropwise (Zymark). The resultant reaction mixture was stirred overnight at room temperature. The solvent was evaporated. The residue was purified by high-performance liquid chromatography. The product fractions were collected and the solvent was evaporated, yielding O.Olόg of compound (8) (S-configuration).
Example B 8 Preparation of compound (9)
Figure imgf000063_0001
Lithium hydroxide (0.340 g, 0.0081 mol) was added to a mixture of intermediate (44) (0.0006 mol) in CH3OH (25ml) and H2O (5ml), stirred at room temperature. The reaction mixture was stirred for one hour at 4O0C. The mixture was concentrated under reduced pressure to one fifth of the initial volume. The concentrate was poured into water. The mixture was stirred for 30 minutes at room temperature. The precipitate was filtered off, stirred in THF (20ml) for one hour, then the precipitate was filtered off again. The solid was dissolved in THF/CH3OH 1/1 (200ml). The whole was filtered and the filtrate was evaporated under reduced pressure. The residue was dried, then stirred for one hour in CH3CN. The precipitate was filtered off and dried, yielding 0.142g (48%) of compound (9).
Example B 9 Preparation of compound (10)
Figure imgf000063_0002
To a stirred mixture Of Cs2CO3 (0.018 mol), CH3CN (100ml) and ΛWN-tributyl-l- butanaminium iodide (0.0072 mol) was added a solution of intermediate (50) (0.0036 mol) in CH3CN (300ml) at 6O0C. The reaction mixture was stirred for 4 hours at 6O0C. The solvent was evaporated under reduced pressure. The residue was purified by high-performance liquid chromatography. The product fractions were collected and the solvent was evaporated, yielding 1.4g of compound (10). Example BlO Preparation of compound (11)
Figure imgf000064_0001
A mixture of intermediate (42) (0.0017 mol), (3-hydroxypropyl)-l,l-dimethylethyl ester carbamic acid (0.0041 mol) and triphenylphosphine (0.0038 mol) in THF (20ml) was stirred at room temperature. DIAD (0.004 mol) was added dropwise and the reaction mixture was stirred for 1 hour at room temperature. The solvent was evaporated and the residue was stirred up in CH3CN (50ml). The precipitate was filtered off, washed with CH3CN and dried, yielding 0.815g (80%) of compound (11).
Example BIl Preparation of compound (12)
Figure imgf000064_0002
A mixture of intermediate (51) (0.00032 mol) and 1,1-dioxidethiomorpholine (0.00185 mol) in 2-propanol (2ml) was stirred for 2 hours at 70°C. DMF (2ml) was added and the resultant reaction mixture was stirred for 16 hours at 70°C. The reaction mixture was cooled at room temperature slowly. The precipitate was filtered off and dried, yielding 0.108g (53%) of compound (12) (R-configuration).
Example B 12 Preparation of compound (13)
Figure imgf000065_0001
Intermediate (52) (0.003190 mol) was stirred in CH3CN (20ml). N-(2-aminoethyl)acetamide (2ml) was added and the resultant reaction mixture was stirred overnight at room temperature. K2CO3 (aq.) (0.009569 mol) was added and the reaction mixture was stirred and refluxed for 2 hours, then cooled to room temperature and the solvent was evaporated in vacuo. Water was added to the residue and this mixture was stirred for 30 minutes at room temperature. The yellow precipitate was filtered off and dried. This fraction was purified by flash column chromatography over a Biotage cartridge (eluent: CH2C12/(CH3OH/NH3) 95/5 up to 80/20). The product fractions were collected and the solvent was evaporated, yielding 0.94g of compound (13).
Example B 13 Preparation of compound (14)
Figure imgf000065_0002
Intermediate (52) (0.003544 mol) was stirred in CH3CN (20ml). 2-(Methylsulfonyl)ethanamine hydrochloride (0.007088 mol) was added. K2CO3 (aq.)
(0.0106 mol) was added and the reaction mixture was stirred and refluxed overnight, then cooled to room temperature and the solvent was evaporated in vacuo. Water was added to the residue and this mixture was stirred for 10 minutes at room temperature.
The yellow precipitate was filtered off and dried. This fraction was purified by flash column chromatography over a Biotage cartridge (eluent: CH2C12/(CH3OH/NH3) from
100/0 to 94/6). The product fractions were collected and the solvent was evaporated, yielding 1.24g (58%) of compound (14). Table F-I lists the compounds that were prepared according to one of the above Examples. The following abbreviations were used in the tables : M.P. stands for the melting point.
Table F-I
Figure imgf000066_0001
Compound identification
LCMS-methods;
The HPLC gradient was supplied by a Waters Alliance HT 2790 system with a column heater set at 4O0C. Flow from the column was split to a Waters 996 photodiode array (PDA) detector and a Waters-Micromass ZQ mass spectrometer with an electrospray ionization source operated in positive and negative ionization mode.
Method 1:
Reversed phase HPLC was carried out on a Xterra MS C18 column (3.5 mm, 4.6 x 100 mm) with a flow rate of 1.6 ml/min. Three mobile phases (mobile phase A 95% 25mM ammonium acetate + 5% acetonitrile; mobile phase B: acetonitrile; mobile phase C: methanol) were employed to run a gradient condition from 100 % A to 50% B and 50% C in 6.5 minutes, to 100 % B in 1 minute, 100% B for 1 minute and reequilibrate with 100 % A for 1.5 minutes. An injection volume of 10 uL was used.
Method 2:
Reversed phase HPLC was carried out on a Chromolith (4.6 x 25 mm) with a flow rate of 3 ml/min. Three mobile phases (mobile phase A 95% 25mM ammoniumacetate + 5% acetonitrile; mobile phase B: acetonitrile; mobile phase C: methanol) were employed to run a gradient condition from 96 % A to 2% B and 2% C in 0.9 minutes, to 49 % B and 49 % C in 0.3 minute, 100% B for 0.2 minute. An injection volume of 2 uL was used.
Method 3:
Reversed phase HPLC was carried out on a Xterra MS Cl 8 column (3.5 mm, 4.6 x 100 mm) with a flow rate of 1.6 ml/min. Two mobile phases (mobile phase A methanol/H2O; mobile phase B 0.1 % formic acid) were employed to run a gradient condition from 100 % B to 5 % B 12 minutes. An injection volume of 10 uL was used.
Method 4:
Reversed phase HPLC was carried out on a Xterra MS C18 column (3.5 mm, 4.6 x 100 mm) with a flow rate of 1.6 ml/min. Three mobile phases (mobile phase A 95% 25mM ammonium acetate + 5% acetonitrile; mobile phase B: acetonitrile; mobile phase C: methanol) were employed to run a gradient condition from 100 % A to 30% A, 35 % B; 35 % C in 3 minutes to 50 % B and 50% C in 3.5 minutes, to 100 % B in 0.5 minute. An injection volume of 10 uL was used.
Method 5:
Reversed phase HPLC was carried out on a Kromasil C18 column (3.5 mm, 4.6 x 100 mm) with a flow rate of 1 ml/min. Three mobile phases (mobile phase A ammonium acetate; mobile phase B: acetonitrile; mobile phase C: formic acid) were employed to run a gradient condition from 30 % A, 40 % B, 30 % C for 1 minute to 100 % B for 5 minutes. An injection volume of 10 uL was used.
Table : retention time (RT in minutes) and molecular weight as the MH+
Figure imgf000068_0001
Figure imgf000069_0001
C. Pharmacological examples
Cl Kinase profiling
The in vitro inhibition of a panel of kinases was assessed using the glass-fiber filter technology as described by Davies, S.P. et al., Biochem J. (2000), 351; p.95-105.
In the glass-fiber filter technology the activity of the kinase of interest is measured using an appropriate substrate that is incubated with the aforementioned kinase protein in the presence of (33P) radiolabeled ATP. (33P) Phosporylation of the substrate is subsequently measured as radioactivity bound on a glassfiber-filter.
Detailed description
All kinases are pre-diluted to a 10x working concentration prior to addition into the assay. The composition of the dilution buffer for each kinase is detailed below.
Figure imgf000069_0002
All substrates are dissolved and diluted to working stocks in de-ionised water.
AbI human In a final reaction volume of 25 μl, AbI (h) (5-10 mU) is incubated with 8 mM MOPS pH 7.0, 0.2 mM EDTA, 50 μM EAIYAAPFAKKK, 10 mM MgAcetate and [γ-33P- ATP] (specific activity approx. 500 cpm/pmol, concentration as required). The reaction is initiated by the addition of the MgATP mix. After incubation for 40 minutes at room temperature, the reaction is stopped by the addition of 5 μl of a 3% phosphoric acid solution. 10 μl of the reaction is then spotted onto a P30 filtermat and washed three times for 5 minutes in 75 mM phosphoric acid and once in methanol prior to drying and scintillation counting.
BIk mouse
In a final reaction volume of 25 μl, BIk (m) (5-10 mU) is incubated with 50 mM Tris pH 7.5, 0.1 mM EGTA, 0.1 mM Na3VO4, 0.1% β-mercaptoethanol, 0.1 mg/ml poly(Glu, Tyr) 4:1, 10 mM MgAcetate and [γ-33P-ATP] (specific activity approx. 500 cpm/pmol, concentration as required). The reaction is initiated by the addition of the MgATP mix. After incubation for 40 minutes at room temperature, the reaction is stopped by the addition of 5 μl of a 3% phosphoric acid solution. 10 μl of the reaction is then spotted onto a Filtermat A and washed three times for 5 minutes in 75 mM phosphoric acid and once in methanol prior to drying and scintillation counting.
Bmx human
In a final reaction volume of 25 μl, Bmx (h) (5-10 mU) is incubated with 8 mM MOPS pH 7.0, 0.2 mM EDTA, 0.1 mg/ml poly(Glu, Tyr) 4:1, 10 mM MgAcetate and [γ-33P- ATP] (specific activity approx. 500 cpm/pmol, concentration as required). The reaction is initiated by the addition of the MgATP mix. After incubation for 40 minutes at room temperature, the reaction is stopped by the addition of 5 μl of a 3% phosphoric acid solution. 10 μl of the reaction is then spotted onto a Filtermat A and washed three times for 5 minutes in 75 mM phosphoric acid and once in methanol prior to drying and scintillation counting.
CDK5/p35 human
In a final reaction volume of 25 μl, CDK5/p35 human (5-10 mU) is incubated with 8 mM MOPS pH 7.0, 0.2 mM EDTA, 0.1 mg/ml histone Hl, 10 mM MgAcetate and [γ- 33P-ATP] (specific activity approx. 500cpm/pmol, concentration as required). The reaction is initiated by the addition of the MgATP mix. After incubation for 40 minutes at room temperature, the reaction is stopped by the addition of 5 μl of a 3% phosphoric acid solution. 10 μl of the reaction is then spotted onto a P30 filtermat and washed three times for 5 minutes in 75 mM phosphoric acid and once in methanol prior to drying and scintillation counting.
CDK6/cvclinD3 human In a final reaction volume of 25 μl, CDK6/cyclinD3 human (5-10 mU) is incubated with 8 mM MOPS pH 7.0, 0.2 mM EDTA, 0.1 mg/ml histone Hl, 10 mM MgAcetate and [γ-33P-ATP] (specific activity approx. 500 cpm/pmol, concentration as required). The reaction is initiated by the addition of the MgATP mix. After incubation for 40 minutes at room temperature, the reaction is stopped by the addition of 5 μl of a 3% phosphoric acid solution. 10 μl of the reaction is then spotted onto a P30 filtermat and washed three times for 5 minutes in 75 mM phosphoric acid and once in methanol prior to drying and scintillation counting.
cSRC human In a final reaction volume of 25 μl, cSRC (h) (5-10 mU) is incubated with 8 mM
MOPS pH 7.0, 0.2 mM EDTA, 250 μM KVEKIGEGTYGVVYK (Cdc2 peptide), 10 mM MgAcetate and [γ- P-ATP] (specific activity approx. 500 cpm/pmol, concentration as required). The reaction is initiated by the addition of the MgATP mix. After incubation for 40 minutes at room temperature, the reaction is stopped by the addition of 5 μl of a 3% phosphoric acid solution.
10 μl of the reaction is then spotted onto a P30 filtermat and washed three times for 5 minutes in 75 mM phosphoric acid and once in methanol prior to drying and scintillation counting.
EGFR human
In a final reaction volume of 25 μl, EGFR (h) (5-10 mU) is incubated with 8 mM MOPS pH 7.0, 0.2 mM EDTA, 1OmM MnC12, 0.1 mg/ml poly(Glu, Tyr) 4:1, 10 mM
MgAcetate and [γ- P-ATP] (specific activity approx. 500 cpm/pmol, concentration as required). The reaction is initiated by the addition of the MgATP mix. After incubation for 40 minutes at room temperature, the reaction is stopped by the addition of 5 μl of a 3% phosphoric acid solution. 10 μl of the reaction is then spotted onto a Filtermat A and washed three times for 5 minutes in 75 mM phosphoric acid and once in methanol prior to drying and scintillation counting.
ErbB4 human
In a final reaction volume of 25 μl, ErbB4 (h) (5-10 mU) is incubated with 8 mM MOPS pH 7.0, 0.2 mM EDTA, 10 mM MnC12, 0.1 mg/ml poly(Glu, Tyr) 4:1, 10 mM MgAcetate and [γ-33P-ATP] (specific activity approx. 500 cpm/pmol, concentration as required). The reaction is initiated by the addition of the MgATP mix. After incubation for 40 minutes at room temperature, the reaction is stopped by the addition of 5 μl of a 3% phosphoric acid solution. 10 μl of the reaction is then spotted onto a Filtermat A and washed three times for 5 minutes in 75 mM phosphoric acid and once in methanol prior to drying and scintillation counting.
Fgr human
In a final reaction volume of 25 μl, Fgr human (5-10 mU) is incubated with 8 mM MOPS pH 7.0, 0.2 mM EDTA, 0.1 mg/ml poly(Glu, Tyr) 4:1, 10 mM MgAcetate and [γ-33P-ATP] (specific activity approx. 500 cpm/pmol, concentration as required). The reaction is initiated by the addition of the MgATP mix. After incubation for 40 minutes at room temperature, the reaction is stopped by the addition of 5 μl of a 3% phosphoric acid solution. 10 μl of the reaction is then spotted onto a Filtermat A and washed three times for 5 minutes in 75 mM phosphoric acid and once in methanol prior to drying and scintillation counting.
Fyn human
In a final reaction volume of 25 μl, Fyn human (5-10 mU) is incubated with 50 mM Tris pH 7.5, 0.1 mM EGTA, 0.1 mM Na3VO4, 250 μM KVEKIGEGTYGVVYK (Cdc2 peptide), 10 mM MgAcetate and [γ-33P-ATP] (specific activity approx. 500 cpm/pmol, concentration as required). The reaction is initiated by the addition of the MgATP mix.
After incubation for 40 minutes at room temperature, the reaction is stopped by the addition of 5 μl of a 3% phosphoric acid solution. 10 μl of the reaction is then spotted onto a P30 filtermat and washed three times for 5 minutes in 75 mM phosphoric acid and once in methanol prior to drying and scintillation counting.
Lck human
In a final reaction volume of 25 μl, Lck (h) (5-10 mU) is incubated with 50 mM Tris pH 7.5, 0.1 mM EGTA, 0.1 mM Na3VO4, 250 μM KVEKIGEGTYGVVYK (Cdc2 peptide), 10 mM MgAcetate and [γ-33P-ATP] (specific activity approx. 500 cpm/pmol, concentration as required). The reaction is initiated by the addition of the MgATP mix.
After incubation for 40 minutes at room temperature, the reaction is stopped by the addition of 5 μl of a 3% phosphoric acid solution. 10 μl of the reaction is then spotted onto a P30 filtermat and washed three times for 5 minutes in 75 mM phosphoric acid and once in methanol prior to drying and scintillation counting. Lyn human
In a final reaction volume of 25 μl, Lyn (h) (5-10 mU) is incubated with 50 mM Tris pH 7.5, 0.1 mM EGTA, 0.1 mM Na3VO4, 0.1% β-mercaptoethanol, 0.1 mg/ml poly(Glu, Tyr) 4:1, 10 mM MgAcetate and [γ-33P-ATP] (specific activity approx. 500 cpm/pmol, concentration as required). The reaction is initiated by the addition of the MgATP mix. After incubation for 40 minutes at room temperature, the reaction is stopped by the addition of 5 μl of a 3% phosphoric acid solution. 10 μl of the reaction is then spotted onto a Filtermat A and washed three times for 5 minutes in 75 mM phosphoric acid and once in methanol prior to drying and scintillation counting.
Ret human
In a final reaction volume of 25 μl, Ret human (5-10 mU) is incubated with 8 mM MOPS pH 7.0, 0.2 mM EDTA, 250 μM KKKSPGEYVNIEFG, 10 mM MgAcetate and [γ-33P-ATP] (specific activity approx. 500 cpm/pmol, concentration as required). The reaction is initiated by the addition of the MgATP mix. After incubation for 40 minutes at room temperature, the reaction is stopped by the addition of 5 μl of a 3% phosphoric acid solution. 10 μl of the reaction is then spotted onto a P30 filtermat and washed three times for 5 minutes in 75 mM phosphoric acid and once in methanol prior to drying and scintillation counting.
Yes human
In a final reaction volume of 25 μl, Yes (h) (5-10 mU) is incubated with 8 mM MOPS pH 7.0, 0.2 mM EDTA, 0.1 mg/ml poly(Glu, Tyr) 4:1, 10 mM MgAcetate and [γ-33P- ATP] (specific activity approx. 500 cpm/pmol, concentration as required). The reaction is initiated by the addition of the MgATP mix. After incubation for 40 minutes at room temperature, the reaction is stopped by the addition of 5 μl of a 3% phosphoric acid solution. 10 μl of the reaction is then spotted onto a Filtermat A and washed three times for 5 minutes in 75 mM phosphoric acid and once in methanol prior to drying and scintillation counting.
Fltl human
In a final reaction volume of 25 μl, Fltl human (5-10 mU) is incubated with 8 mM MOPS pH 7.0, 0.2 mM EDTA, 250 μM KKKSPGEYVNIEFG, 10 mM MgAcetate and [γ-33P-ATP] (specific activity approx. 500 cpm/pmol, concentration as required). The reaction is initiated by the addition of the MgATP mix. After incubation for 40 minutes at room temperature, the reaction is stopped by the addition of 5 μl of a 3% phosphoric acid solution. 10 μl of the reaction is then spotted onto a P30 filtermat and washed three times for 5 minutes in 75 mM phosphoπc acid and once in methanol pπor to drying and scintillation counting.
Hck human
In a final reaction volume of 25 μl, Hck human (5-10 mU) is incubated with 8 mM MOPS pH 7.0, 0.2 mM EDTA, 250 μM KVEKIGEGTYGVVYK (Cdc2 peptide), 10 mM MgAcetate and [γ-33P-ATP] (specific activity approx. 500 cpm/pmol, concentration as required) The reaction is initiated by the addition of the MgATP mix After incubation for 40 minutes at room temperature, the reaction is stopped by the addition of 5 μl of a 3% phosphoπc acid solution 10 μl of the reaction is then spotted onto a P30 filtermat and washed three times for 5 minutes in 75 mM phosphoπc acid and once in methanol pπor to drying and scintillation counting.
The following tables provides the scores for the compounds according to the invention, obtained at a test concentration of 106 M using the above mentioned kinase assays
Score 1 = 10-30% inhibition, Score 2 = 30-60% inhibition, Score 3 = 60-80% inhibition and Score 4 = > 80% inhibition.
Figure imgf000074_0001
Figure imgf000075_0002
Figure imgf000075_0001
D. Composition examples
The following formulations exemplify typical pharmaceutical compositions suitable for systemic administration to animal and human subjects in accordance with the present invention. "Active ingredient" (A.I.) as used throughout these examples relates to a compound of formula (I) or a pharmaceutically acceptable addition salt thereof.
Example D.I : film-coated tablets Preparati.on.of tablet core A mixture of A.I. (100 g), lactose (570 g) and starch (200 g) was mixed well and thereafter humidified with a solution of sodium dodecyl sulfate (5 g) and polyvinylpyrrolidone (10 g) in about 200ml of water. The wet powder mixture was sieved, dried and sieved again. Then there was added microcrystalline cellulose (100 g) and hydrogenated vegetable oil (15 g). The whole was mixed well and compressed into tablets, giving 10.000 tablets, each comprising 10 mg of the active ingredient. Coating
To a solution of methyl cellulose (10 g) in denaturated ethanol (75ml) there was added a solution of ethyl cellulose (5 g) in DCM (150ml). Then there were added DCM (75ml) and 1,2,3-propanetriol (2.5ml). Polyethylene glycol (10 g) was molten and dissolved in dichloromethane (75ml). The latter solution was added to the former and then there were added magnesium octadecanoate (2.5 g), polyvinyl-pyrrolidone (5 g) and concentrated color suspension (30ml) and the whole was homogenated. The tablet cores were coated with the thus obtained mixture in a coating apparatus.

Claims

Claims
1. A compound having the formula
Figure imgf000077_0001
the iV-oxide forms, the pharmaceutically acceptable addition salts and the stereochemically isomeric forms thereof, wherein
Z represents NH; Y represents -Cj.galkyl-, -C2-9alkenyl-, -d.salkyl-oxy-d-salkyl-,
Figure imgf000077_0002
-NH-CO-d_6alkyl-, -CO-C1-7alkyl-, -C1-7alkyl-CO-, C1-6alkyl-CO-C1-6alkyl, -Ci.zalkyl-NR^-CO-CR^R'^NH^ -d-zalkyl-CO-NH-CR^R^-CO-, -d.zalkyl-CO-NR^-d.salkyl-CO^ -Ci^alkyl-NR^-CHz-CO-NH-CLsalkyl-, -NR22-CO-C1-3alkyl-NH-, -C1-3alkyl-NH-CO-Het20-, C1-2alkyl-CO-Het21-CO-, or
-Het22-CH2-CO-NH-C1-3alkyl-;
X1 represents O, -O-Ci-2alkyl-, -0-N=CH-, NR11 or -NRu-C1-2alkyl-; X2 represents a direct bond, d_2alkyl, O, -O-C1-2alkyl-, CO, -CO-C1-2alkyl-, -0-N=CH-
, NR12 or NR12-C1-2alkyl-; R1 represents hydrogen, cyano, halo or hydroxy, preferably halo; R2 represents hydrogen, cyano, halo, hydroxy, hydroxycarbonyl-,
C1-4alkyloxycarbonyl-, Het16-carbonyl-, d_4alkyl-, C2-6alkynyl-, Ar5, Het1 or dihydroxyborane;
R3 represents hydrogen, cyano, halo, hydroxy, formyl,
Figure imgf000077_0003
C1-6alkyl-, C1-6alkoxy- substituted with halo, or R3 represents C1-4alkyl substituted with one or where possible two or more substituents selected from hydroxy or halo; R4 represents Ar4-C1-4alkyloxy-, d-4alkyloxy- or R4 represents d.4alkyloxy substituted with one or where possible two or more substituents selected from hydroxy-, halo, Ci-4alkyloxy-, d_4alkyloxy-C1-4alkyloxy-, NR37R38-carbonyloxy-, Het5- carbonyloxy-, NR7R8, NR9R10-carbonyl-, Het3-carbonyl-, Het13-oxy- or Het2-; R7 represents hydrogen, hydroxy-C1-4alkyl- or Ci-4alkyl;
R8 represents C3_6cycloalkyl; Het6-carbonyl-; Het7-aminocarbonyl-; Het8;
Het9-oxycarbonyl-; Het10-sulfonyl-; C^alkyloxycarbonyl; mono- or diCC^alky^aminocarbonyl-; mono- or di(C1-4alkyl)aminocarbonyl substituted with Ct^alkylsulfonyl-; or
Ci-4alkylcarbonyl optionally substituted with one or more substituents selected from C^alkylsulfonyl, hydroxy- and C^alkyloxy-; or
R8 represents C^alkyl substituted with one or more substituents selected from
Ci-4alkylsulfonyl-, NR25R26, aminocarbonyloxy-, C1-4alkylcarbonyloxy-, aminocarbonyl-, hydroxy-C1-4alkyloxy-, C1-4alkyloxy-C1-4alkyloxy-, and Het11;
R9 represents hydrogen or C1-4alkyl-;
R10 represents Het4 or C1-4alkyl- substituted with C1-4alkylsulfonyl-, ; R11 represents hydrogen, C1-4alkyl- or C1-4alkyl-oxy-carbonyl-; R12 represents hydrogen, C1-4alkyl-, C^alkyloxycarbonyl- or C^alkyloxycarbonyl- substituted with phenyl;
R13 represents hydrogen, Het14-C1-4alkyl, d-όalkyloxycarbonyl optionally substituted with phenyl or R13 represents Ar6-sulfonyl or Het24-Ci-4alkylcarbonyl; R14 and R15 are each independently selected from hydrogen, C1-4alkyl,
Het15-C1.4alkyl- or C1-4alkyloxyC1-4alkyl-; R16 and R17 each independently represents hydrogen, C1-4alkyl or C1-4alkyl substituted with hydroxy-, C3-6cycloalkyl or phenyl; or R16 and R17 taken together with the carbon atom to which they are attached form a C3-6cycloalkyl; R18 represents hydrogen or Ci-4alkyl optionally substituted with hydroxy or phenyl; R19 represents hydrogen or Chalky]; R20 represents hydrogen or Chalky];
R represents hydrogen, Ci-4alkyl, Het -Ci_4alkylcarbonyl- or
R21 represents mono-or di(C1-4alkyl)amino-C1-4alkyl-carbonyl- optionally substituted with hydroxy, pyrimidinyl, dimethylamine or C1-4alkyloxy; R22 represents hydrogen or Chalky] optionally substituted with hydroxy or Ci_4alkyloxy;
R23 represents C1-4alkyl optionally substituted with hydroxy-, C1-4alkyloxy- or Het25;
R23 may also represent hydrogen when R16 and R17 taken together with the carbon atom to which they are attached form a C3-6cycloalkyl;
R25 and R26 each independently represent hydrogen, C^alkyl, Ci.4alkylsulfonyl-, aminocarbonyl-, mono- or diCCt^alkyOaminocarbonyl-, C1-4alkylcarbonyl-,
C1-4alkyloxycarbonyl- or C1-4alkyl substituted with one or more substituents selected from C1-4alkylsulfonyl-, hydroxy- and Ci-4alkyloxy-; R27 and R28 each independently represent hydrogen, C1-4alkyl, C^alkylsulfonyl-, aminocarbonyl-, mono- or di(C1-4alkyl)aminocarbonyl-, Ci_4alkylcarbonyl-, C1-4alkyloxycarbonyl- or C^alkyl substituted with one or more substituents selected from C1-4alkylsulfonyl-, hydroxy- and d_4alkyloxy-; or for those compounds of formula (I) wherein Het represents a heterocycle selected from morpholinyl, piperazinyl, piperidinyl pyrrolidinyl or thiomoφholinyl substituted with NR27R28-C1-4alkyl said R27 and R28 each independently represent Ci_4alkylsulfonyl-, aminocarbonyl-, mono- or di(C1-4alkyl)aminocarbonyl-, C1-4alkylcarbonyl-, Ci-4alkyloxycarbonyl- or C1-4alkyl substituted with one or more substituents selected from C1-4alkylsulfonyl-, hydroxy- and Q^alkyloxy-;
R29 and R30 each independently represent hydrogen, aminosulfonyl, aminocarbonyl, mono- or di(C1-4alkyl)aminocarbonyl-, mono- or di(C1-4alkyl)aminosulfonyl-, or C^alkyl- optionally substituted with one or more substituents selected from NR31R32, C^alkylsulfonyl, aminocarbonyloxy-, hydroxy-, C1-4alkyloxy-, aminocarbonyl- and mono- or di(Ci-4alkyl)aminocarbonyl-, or
C^alkyloxycarbonyl optionally substituted with one or more substituents selected from hydroxy, Ci^alkyloxy- and Ci^alkylsulfonyl-, or C1-4alkylcarbonyl optionally substituted with one or more substituents selected from hydroxy-, C1-4alkyloxy- and C^alkylsulfonyl-; R31 and R32 each independently represent hydrogen, C^alkyl, C^alkylsulfonyl-, aminocarbonyl-, mono- or di(Ci-4alkyl)aminocarbonyl-, C1-4alkylcarbonyl-, C^alkyloxycarbonyl- or C1-4alkyl substituted with one or more substituents selected from C1-4alkylsulfonyl-, hydroxy- and C^alkyloxy-; R33 represents hydrogen or C1-4alkyl; R34 represents C1-4alkylsulfonyl-, aminocarbonyl-, mono- or
Figure imgf000079_0001
Ci-4alkyloxycarbonyl- or Ci.4alkyl substituted with one or more substituents selected from C1-4alkylsulfonyl- , hydroxy- and C^alkyloxy-; R35 represents hydrogen or C^alkyl; R36 represents C1-4alkylsulfonyl-, aminocarbonyl-, mono- or
Figure imgf000079_0002
C1-4alkyloxycarbonyl- or C1-4alkyl substituted with one or more substituents selected from C^alkylsulfonyl- , hydroxy- and C1-4alkyloxy-; R37 and R38 each independently represent hydrogen, C1-4alkyl, Ci_4alkylsulfonyl-, Het12 or C1-4alkyl substituted with one or more substituents selected from
C1-4alkylsulfonyl-, hydroxy- and C1-4alkyloxy-; R39 and R40 each independently represent aminosulfonyl, aminocarbonyl, mono- or di(Ci_4alkyl)aminocarbonyl-, mono- or di(C1-4alkyl)aminosulfonyl-, or
C1-4alkyl- substituted with one or more substituents selected from NR31R32,
C1-4alkylsulfonyl, aminocarbonyloxy-, hydroxy-, C1-4alkyloxy-, aminocarbonyl- and mono- or di(C1-4alkyl)aminocarbonyl-, or
C1-4alkyloxycarbonyl optionally substituted with one or more substituents selected from hydroxy, Ci_4alkyloxy- and C1-4alkylsulfonyl-, or
C1-4alkylcarbonyl optionally substituted with one or more substituents selected from hydroxy-, C1-4alkyloxy- and C^alkylsulfonyl-; Het1 represents thiazolyl or 2-bora-l,3-dioxolanyl wherein said Het1 is optionally substituted with one or where possible two, three, four or more substituents selected from amino, C^alkyl, hydroxy-C1-4alkyl-, phenyl, phenyl-C1-4alkyl-, d^alkyl-oxy-C^alkyl- mono- or di (C1 -4alkyl) amino- or amino-carbonyl-; Het2 represents a heterocycle selected from tetrahydropyranyl, tetrahydrofuranyl, furanyl, 1,1-dioxothiomorpholinyl, piperazininonyl, tetrahydro-l,l-dioxido-2H- thiopyranyl, piperidinonyl, azetidinyl or 2-azetidinonyl wherein said Het2 is optionally substituted with one or where possible two or more substituents selected from hydroxy, amino, NR29R30, aminocarbonyl, mono- or di(C1_4alkyl)aminocarbonyl, Q^alkylsulfonyl or C1-4alkyl- optionally substituted with one or more substituents selected from
NR27R28, C1-4alkylsulfonyl, aminocarbonyloxy-, aminocarbonyl- and mono- or di(C1-4alkyl)aminocarbonyl-, or
C^alkyloxy- optionally substituted with C^alkyloxy-, or
Ci_4alkyloxycarbonyl optionally substituted with one or more substituents selected from hydroxy, C1-4alkyloxy- and C1-4alkylsulfonyl-, or
Ci-4alkylcarbonyl optionally substituted with one or more substituents selected from hydroxy-, C1-4alkyloxy- and C1-4alkylsulfonyl-; or Het2 represents a heterocycle selected from morpholinyl, piperazinyl, piperidinyl, pyrrolidinyl, thiomorpholinyl or 1,1-dioxothiomorpholinyl wherein said Het2 is optionally substituted with one or where possible two or more substituents selected from
C!_4alkyl- optionally substituted with one or more substituents selected from
NR27R28, C1-4alkylsulfonyl, aminocarbonyloxy-, aminocarbonyl- and mono- or di(C1-4alkyl)aminocarbonyl-, or C1-4alkyloxy- optionally substituted with C1-4alkyloxy-, or
C1-4alkyloxycarbonyl optionally substituted with one or more substituents selected from hydroxy, Ci-4alkyloxy- and C^alkylsulfonyl-, or Ci_4alkylcarbonyl optionally substituted with one or more substituents selected from hydroxy-, Ci-4alkyloxy- and C^alkylsulfonyl-; Het3 represents a heterocycle selected from tetrahydropyranyl, tetrahydrofuranyl, furanyl, 1,1-dioxothiomorpholinyl, piperazininonyl, tetrahydro-l,l-dioxido-2H- thiopyranyl, piped dinonyl, azetidinyl or 2-azetidinonyl wherein said Het3 is optionally substituted with one or where possible two or more substituents hydroxy-, amino, C1-4alkyl-, C3.6cycloalkyl-C1-4alkyl-, aminosulfonyl-, mono- or dKC^alkyOaminosulfonyl-, amino-C1-4alkyl-, Mono- or di(C1-4alkyl)amino- d-4alkyl, NR35R36, C^alkyl-sulfonyl-C^alkyl- or C^alkyloxy- optionally substituted with C1-4alkyloxy- or hydroxy; or
Het3 represents a heterocycle selected from morpholinyl, piperazinyl, piperidinyl, furanyl or pyrrolidinyl wherein said Het3 is substituted with one or where possible two or more substituents selected from NR35R36, C1-4alkyl-sulfonyl-C1-4alkyl- or C1-4alkyloxy- optionally substituted with C1-4alkyloxy- or hydroxy; Het4 represents a heterocycle selected from morpholinyl, piperazinyl, piperidinyl, furanyl, pyrazolyl, dioxolanyl, thiazolyl, oxazolyl, imidazolyl, isoxazolyl, oxadiazolyl, pyridinyl or pyrrolidinyl wherein said Het4 is substituted with one or where possible two or more substituents selected from Ci-4alkyl-sulfonyl- C1-4alkyl-, C1-4alkyloxy- optionally substituted with C1-4alkyloxy- or hydroxy; Het5 represents a heterocycle selected from furanyl, piperazinyl, 1,1- dioxothiomorpholinyl, piperazininonyl, piperidinyl, tetrahydro-l,l-dioxido-2H- thiopyranyl, piperidinonyl, morpholinyl or pyrrolidinyl wherein said Het5 is optionally substituted with hydroxy, amino, mono- or di(C1-4alkyl)-amino-, d.4alkyl, Het6 and Het7 each independently represents a heterocycle selected from piperazinyl, piperidinyl or pyrrolidinyl wherein said heterocycles are optionally substituted with one or more substituents selected from hydroxy-, amino, hydroxy-C1-4alkyl-, C1-4alkyloxy-C1-4alkyl- and C1-4alkyl-; Het8 represents a heterocycle selected from tetrahydropyranyl, tetrahydrofuranyl, 1,1-dioxothiomorpholinyl, piperazininonyl, tetrahydro-l,l-dioxido-2H- thiopyranyl, piperidinonyl, azetidinyl or 2-azetidinonyl wherein said Het8 is optionally substituted with aminosulfonyl, aminocarbonyl, mono- or di(C1.4alkyl)aminocarbonyl-, mono- or di(C1.4alkyl)aminosulfonyl-, or Ci-4alkyl- optionally substituted with one or more substituents selected from amino, mono- or di(C;[.4alkyl)amino-, NR33R34, C1-4alkylsulfonyl, aminocarbonyloxy-, hydroxy-, C1-4alkyloxy-, aminocarbonyl- and mono- or di(Ci-4alkyl)aminocarbonyl-, or C1-4alkyloxycarbonyl optionally substituted with one or more substituents selected from hydroxy, C^alkyloxy- and Ci_4alkylsulfonyl-, or
C1-4alkylcarbonyl optionally substituted with one or more substituents selected from hydroxy, Ci_4alkyloxy- and Ci_4alkylsulfonyl-; or Het8 represents a heterocycle selected from furanyl, piperidinyl or piperazinyl wherein said Het8 is substituted with aminocarbonyl, mono- or di(C1-4alkyl)aminocarbonyl-, mono- or di(C1.4alkyl)aminosulfonyl-, or
C1-4alkyl- substituted with one or more substituents selected from NR33R34,
C1-4alkylsulfonyl, aminocarbonyloxy-, hydroxy-, C1-4alkyloxy-, aminocarbonyl-
Figure imgf000082_0001
or
C1-4alkyloxycarbonyl optionally substituted with one or more substituents selected from hydroxy, Ci_4alkyloxy- and Ci-4alkylsulfonyl-, or d^alkylcarbonyl optionally substituted with one or more substituents selected from hydroxy, C!_4alkyloxy- and C1-4alkylsulfonyl-; Het9 and Het10 each independently represents a heterocycle selected from piperazinyl, piperidinyl or pyrrolidinyl wherein said heterocycles are optionally substituted with one or more substituents selected from hydroxy-, amino, hydroxy-C1-4alkyl-,
C1-4alkyloxy-C1-4alkyl- and C^alkyl-;
HN. .NH
Het11 represents 2-imidazolidinonyl- or ^ \ ; Het12 represents a heterocycle selected from morpholinyl, piperazinyl, piperidinyl or pyrrolidinyl wherein said Het12 is optionally substituted with one or where possible two or more substituents selected from hydroxy, amino or Q^alkyl-; Het13 represents a heterocycle selected from furanyl, piperazinyl, 1,1- dioxothiomorpholinyl, piperazininonyl, piperidinyl, tetrahydro-l,l-dioxido-2H- thiopyranyl, piperidinonyl, morpholinyl, piperazinyl or pyrrolidinyl;
Het14 and Het15 each independently represent a heterocycle selected from morpholinyl, piperazinyl, piperidinyl or pyrrolidinyl wherein said Het14 and Het15 are optionally substituted with one or where possible two or more substituents selected from hydroxy, amino or Ci_4alkyl; Het16 represents a heterocycle selected from piperidinyl or pyrrolidinyl;
Het20 represents pyrrolidinyl, 2-pyrrolidinonyl, piperidinyl or hydroxy-pyrrolidinyl, preferably pyrrolidinyl or hydroxy-pyrrolidinyl; Het21 represents pyrrolidinyl or hydroxy-pyrrolidinyl; Het22 represents pyrrolidinyl, piperazinyl or piperidinyl; Het23 and Het25 each independently represents a heterocycle selected from morpholinyl, pyrrolidinyl, piperazinyl or piperidinyl wherein said Het .23 is optionally substituted with one or where possible two or more substituents selected from C1-4alkyl, C3-6cycloalkyl, hydroxy-C1-4alkyl-, C^alkyloxyC^alkyl or polyhydroxy- Ci-4EIlCyI-;
Het24 represents morpholinyl, pyrrolidinyl, piperazinyl or piperidinyl; Ar4, Ar5 or Ar6 each independently represent phenyl optionally substituted with nitro, cyano, C1-4alkylsulfonyl-, Ci^alkylsulfonylamino-, aminosulfonylamino-, hydroxy-C1-4alkyl, aminosulfonyl-, hydroxy-, C1-4alkyloxy- or Ci-4alkyl, preferably Ar4 or Ar5 each independently represent phenyl optionally substituted with cyano; further characterised in that either
Y represents -C1.2alkyl-NR23-CO-CR16R17-NH-;
Het1 represents 2-bora-l,3-dioxolanyl optionally substituted with one or where possible two, three, four or more substituents selected from amino, C1-4alkyl, hydroxy-C1-4alkyl-, phenyl, phenyl-C1-4alkyl-,
Figure imgf000083_0001
or di(C1-4alkyl)amino- or amino-carbonyl-;
R13 represents Ci-όalkyloxycarbonyl optionally substituted with phenyl or R13 represents Ar6-sulfonyl or Het24-C1-4alkylcarbonyl; or R4 represents C1-4alkyloxy substituted with at least one substituent selected from
C1-4alkyloxy-Ci-4alkyloxy-, NR37R38-carbonyloxy-, Het5-carbonyloxy-, NR7R8, NR9R10-carbonyl-, Het3-carbonyl-, Het13-oxy- or Het2-; wherein
R8 represents Het7-aminocarbonyl-; Het9-oxycarbonyl-; Het10-sulfonyl-; C1-4alkyloxycarbonyl; mono- or di(C1-4alkyl)aminocarbonyl-; mono- or di(C1-4alkyl)aminocarbonyl substituted with C1-4alkylsulfonyl-; or C1-4alkylcarbonyl optionally substituted with one or more substituents selected from C1-4alkylsulfonyl, hydroxy- and C1-4alkyloxy-; or
R8 represents C1-4alkyl substituted with one or more substituents selected from hydroxy C1-4alkylsulfonyl-, NR25R26, aminocarbonyloxy-, Ci-4alkylcarbonyloxy-, aminocarbonyl-, Ci-4alkyloxy-d-4alkyloxy-, and Het11; Het13 represents d-βalkyloxycarbonyl optionally substituted with phenyl or
R13 represents Ar6-sulfonyl or Het24-d-4alkylcarbonyl; in particular morpholinyl-C1-4alkyl; and Het2 represents a heterocycle selected from morpholinyl, piperazinyl, piperidinyl, pyrrolidinyl or thiomorpholinyl said Het2 substituted with one or where possible two or more substituents selected from
Ci-4alkyl- substituted with one or more substituents selected from NR27R28, C1-4alkylsulfonyl, aminocarbonyloxy-, aminocarbonyl- and mono- or di(Ci-4alkyl)aminocarbonyl-; or d_4alkyloxy- optionally substituted with d_4alkyloxy-; or d-4alkyloxycarbonyl optionally substituted with one or more substituents selected from hydroxy, C1-4alkyloxy- and d.4alkylsulfonyl-; or d-4alkylcarbonyl optionally substituted with one or more substituents selected from hydroxy-, Ci-4alkyloxy- and Ci-4alkylsulfonyl-; or Het2 represents 1,1-dioxothiomorpholinyl optionally substituted with C1-4alkyl- optionally substituted with one or more substituents selected from NR27R28, Ci-4alkylsulfonyl, aminocarbonyloxy-, aminocarbonyl- and mono- or di(Ci_4alkyl)aminocarbonyl-; or
C1-4alkyloxy- optionally substituted with C1-4alkyloxy-; or C1-4alkyloxycarbonyl optionally substituted with one or more substituents selected from hydroxy, C1-4alkyloxy- and d-4alkylsulfonyl-; or C1.4alkylcarbonyl optionally substituted with one or more substituents selected from hydroxy-, C1-4alkyloxy- and Ci-4alkylsulfonyl-.
2. A compound according to claim 1 wherein;
Z represents NH;
Y represents -C3_9alkyl-, -C1-5alkyl-NR13-Ci-5alkyl-, -Ci-5alkyl-NR14-CO-Ci.5alkyl-, -d-ealkyl-CO-NH-,
Figure imgf000084_0001
-C1-2alkyl-NR23-CO-CR16R17-NH-,
-Ci_2alkyl-NR21-CH2-CO-NH-C1_3alkyl or C1-3alkyl-NH-CO-Het20-;
X1 represents a direct bond, O, -O-d-2alkyl-, NR11, or -NRπ-d.2alkyl-;
X2 represents a direct bond, -C1-2alkyl-, CO-C1-2alkyl or NR12-Ci-2alkyl-;
R1 represents hydrogen, cyano, halo or hydroxy; R2 represents hydrogen, halo, cyano, C2-6alkynyl, hydroxy, hydroxycarbonyl,
C1-4alkyloxycarbonyl- or Het1; R represents hydrogen, cyano, halo, hydroxy, formyl, Ci-6alkyloxy or C1-6alkyloxy- substituted with halo;
R4 represents Ar4-C]-4alkyloxy, Ci-4alkyloxy-, or C1-4alkyloxy- substituted with one or where possible two or more substituents selected from hydroxy, Ci_4alkyloxy-,
C1-4alkyloxy-C1-4alkyloxy, NR7R8 or Het2; R7 represents hydrogen, hydroxyC1.4alkyl- or C1-4alkyl; R8 represents d-4alkyloxycarbonyl or C1-4alkyl- substituted with one or more substituents selected from d-4alkylsulfonyl-, d-4alkylcarbonyloxy or NR25R26; in particular R8 represents Ci-4alkyl- substituted with one or more substituents selected from C1-4alkylsulfonyl- or NR25R26; R11 represents hydrogen, Ci_4alkyloxycarbonyl or d^alkyl; R12 represents hydrogen or C^alkyl;
R13 represents C1-6alkyloxycarbonyl optionally substituted with phenyl or R13 represents Ar6-sulfonyl or Het^-d^alkylcarbonyl; R14 and R15 each independently represent hydrogen or C1-4alkyl; R16 and R17 each independently represent hydrogen or Ci-4alkyl optionally substituted with C3.6cycloalkyl or R16 and R 7 taken together with the carbon atom to which they are attached form a C3-6cycloalkyl; R21 represents hydrogen or C1-4alkyloxycarbonyl;
R23 represents C^alkyl optionally substituted with hydroxy-, Ci-4alkyloxy- or Het25; R23 may also represent hydrogen when R16 and R17 taken together with the carbon atom to which they are attached form a C3_6cycloalkyl; R25 and R26 each independently represent hydrogen, C1-4alkyl, Ci_4alkylsulfonyl,
C1-4alkyloxycarbonyl or C1-4alkylcarbonyl;
R27 and R28 each independently represent hydrogen, C1-4alkyl, C1-4alkylsulfonyl, C1-4alkyloxycarbonyl or C1-4alkylcarbonyl;
Het1 represents 2-bora-l,3-dioxolanyl- optionally substituted with one or where possible two, three, four or more substituents selected from amino, d.4alkyl, hydroxy-C1-4alkyl-, phenyl, phenyl-d_4alkyl,
Figure imgf000085_0001
mono- or di(C1-4alkyl)amino- or aminocarbonyl-; Het2 represents 1,1-dioxothiomorpholinyl optionally substituted with
C1-4alkyloxycarbonyl or C1-4alkyl-NR27R28; or Het2 represents piperidinyl or piperazinyl substituted with C1-4alkyloxycarbonyl or -d-4alkyl-NR27R28; Het20 represents pyrrolidinyl, 2-pyrrolidinonyl, piperidinyl or hydroxy-pyrrolidinyl; Het25 represents a heterocycle selected from morpholinyl or piperazinyl wherein said heterocycle is optionally substituted with C1-4alkyl, hydroxy-Ci-4alkyl,
C1-4alkyloxy-C1-4alkyl or polyhydroxy-C1-4alkyl; or Ar4, Ar5 or Ar6 each independently represents phenyl optionally substituted with nitro, cyano, hydroxy, hydroxyd-4alkyl, C1-4alkyl or C1-4alkyloxy; further characterised in that either Y represents -C1-2alkyl-NR23-CO-CR16R17-NH-; or
R4 represents Ci-4alkyloxy substituted with at least one substituent selected from C1-4alkyloxy-C1-4alkyloxy-, NR7R8 or Het2.
3. A compound according to claims 1 or 2 wherein; Z represents NH; Y represents -C3.9a.kyl-, -C1_5alkyl-NR13-C1-5alkyl-,
Figure imgf000086_0001
-C^alkyl-CO-NH-, -Ci^alkyl-NH-CO-, -Ci-2alkyl-NR23-CO-CR16R17-NH-,
-Ci.2alkyl-NR21-CH2-CO-NH-Ci-3alkyl or C1-3alkyl-NH-CO-Het20-; X1 represents a direct bond, O, -O-C^alkyl-, NR11, or -NRn-C1-2alkyl-; X2 represents a direct bond, -Ci-2alkyl-, CO-C1-2alkyl or NR12-Ci_2alkyl-; R1 represents hydrogen or halo;
R2 represents hydrogen, halo, C2-6alkynyl, cyano or Het1; R3 represents hydrogen;
R4 represents Ar4-C1-4alkyloxy, Ci-4alkyloxy-, or C1-4alkyloxy- substituted with one or where possible two or more substituents selected from hydroxy, C1-4alkyloxy-,
C1-4alkyloxy-C1-4alkyloxy, NR7R8 or Het2; R7 represents hydrogen or C1-4alkyl; R8 represents C1-4alkyloxycarbonyl or C^alkyl- substituted with one or more substituents selected from C1-4alkylsulfonyl-, hydroxy, C^alkylcarbonyloxy or NR25R26;
R11 represents hydrogen or C1-4alkyl; R12 represents hydrogen or C1-4alkyl;
R13 represents Ar6-sulfonyl or C1-6alkyloxycarbonyl optionally substituted with phenyl; R14 and R15 represent hydrogen; R16 and R17 each independently represent hydrogen or C^alkyl optionally substituted with C3_6cycloalkyl or R16 and R17 taken together with the carbon atom to which they are attached form a C3-6cycloalkyl; R21 represents hydrogen or Ci-4alkyloxycarbonyl;
R23 represents C1-4alkyl optionally substituted with hydroxy-, C1-4alkyloxy- or Het25; R23 may also represent hydrogen when R16 and R17 taken together with the carbon atom to which they are attached form a C3-6C ycloalkyl; R25 and R26 each independently represent hydrogen or C1-4alkylcarbonyl; R27 and R28 each independently represent hydrogen or C^alkylcarbonyl; Het1 represents 2-bora-l,3-dioxolanyl-; Het2 represents 1,1-dioxothiomorpholinyl, piperidinyl or piperazinyl wherein said Het2 is optionally substituted with C^alkyloxycarbonyl or -C1-4alkyl-NR R ; Het20 represents pyrrolidinyl; Het25 represents a heterocycle selected from morpholinyl or piperazinyl wherein said heterocycle is optionally substituted with C1-4alkyl, hydroxy-C1-4alkyl, C^alkyloxy-C^alkyl or polyhydroxy-C].4alkyl;
Ar4 represents phenyl;
Ar5 represents phenyl; or Ar6 represents phenyl optionally substituted with nitro; further characterised in that either
Y represents -C1-2alkyl-N]R23-CO-CR16R17-NH-; or
R4 represents C^alkyloxy substituted with at least one substituent selected from Cualkyloxy-C^alkyloxy-, NR7R8 or Het2; in particular Ci_4alkyloxy substituted with C^alkyloxy-C^alkyloxy- or NR7R8.
4. A compound according to any one of claims 1 to 3 wherein;
Z represents NH; Y represents -C^alkyK-Q.salkyl-NR^-Ci.salkyl-, -C^alkyl-NR^-CO-d.salkyl-, -C1-2alkyl-NR21-H2-CO-NH-C1-3alkyl- or -Ci.2alkyl-NR23-CO-CR16R17-NH-;
X1 represents O or -O-C1-2alkyl-;
X2 represents a direct bond, C1-2alkyl, -CO-C1-2alkyl or NR^-Q.zalkyl;
R1 represents hydrogen or halo; in particular R1 represents hydrogen; R2 represents halo, acetylene or Het1; in particular R2 represents halo or Het1;
R3 represents hydrogen;
R4 represents Ar4-C1-4alkyloxy-, C1-4alkyloxy- or C1-4alkyloxy substituted with one or where possible two or more substituents selected from Het2, NR7R8, hydroxy and Q^alkyloxy-C^alkyloxy-; R7 represents hydrogen or C1-4alkyl;
R8 represents C1-4alkyl substituted with NR25R26 or Ci-4alkylsulfonyl;
R12 represents hydrogen or C1-4alkyl-;
R13 represents Ar6-sulfonyl or C^alkyloxycarbonyl optionally substituted with phenyl;
R16 and R17 represents hydrogen, C1-4alkyl or R16 and R17 taken together with the carbon atom to which they are attached from a C3-6cycloalkyl;
R23 represents hydrogen or C1-4alkyl;
R25 and R26 each independently represent hydrogen or C1-4alkylcarbonyl;
R27 and R28 each independently represent hydrogen or Ci-4alkylcarbonyl;
Het1 represents 2-bora-l,3-dioxolanyl; Het2 represents piperidinyl, piperazinyl, morpholinyl, thiomorpholinyl or
1,1-dioxothiomorpholinyl wherein said Het2 is optionally substituted with C1-4alkyloxycarbonyl or NR27R28-Ci-4alkyl;
Ar4 represents phenyl;
Ar5 represents phenyl; or Ar6 represents phenyl optionally substituted with nitro.
5. A compound according to any one of claims 1 to 3 wherein; Z represents NH;
Y represents -C^alkyl—Q.salkyl-NR^-Ci-salkyl- or
-Ci-2alkyl-NR23-CO-CR16R17-NH-; X1 represents O; X2 represents a direct bond or NR12-C1-2alkyl-;
R1 represents hydrogen; R2 represents halo or Het1; R3 represents hydrogen; R4 represents Ar4-C1-4alkyloxy-, C1-4alkyloxy- or C1-4alkyloxy substituted with
C i -4alkyloxy-C \ -4alkyloxy- ; R12 represents hydrogen or C1-4alkyl-; R13 represents Ar6-sulfonyl or C1-6alkyloxycarbonyl optionally substituted with phenyl; R16 and R17 taken together with the carbon atom to which they are attached from a
C3-6cycloalkyl;
R23 represents hydrogen or C1-4alkyl; Het1 represents 2-bora-l,3-dioxolanyl;
Ar4 represents phenyl; Ar5 represents phenyl;
Ar6 represents phenyl optionally substituted with nitro.
6. A compound according to any one of claims 1 to 5 wherein X2 substituent is at position 2', the R1 substituent represents hydrogen or halo and is at position 4', the
R2 substituent represents halo and is at position 5', the R3 substituent is at position 2 and the R4 substituent at position 7 of the structure of formula (I)
7. A kinase inhibitor of formula (I).
8. A compound as claimed in any one of claims 1 to 6 for use as a medicine.
9. Use of a compound as claimed in any one of claims 1 to 6 in the manufacture of a medicament for treating cell proliferative disorders such as atherosclerosis, restenosis and cancer.
10. A pharmaceutical composition comprising a pharmaceutically acceptable carrier and, as active ingredient, an effective kinase inhibitory amount of a compound as described in any one of the claims 1 to 6.
PCT/EP2005/056609 2004-12-08 2005-12-08 Macrocyclic quinazole derivatives and their use as mtki WO2006061417A2 (en)

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CA2588764A CA2588764C (en) 2004-12-08 2005-12-08 Mtki quinazoline derivatives
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JP2007544918A JP5140431B2 (en) 2004-12-08 2005-12-08 Macrocyclic quinazole derivatives and their use as MTKI
EP05826362.5A EP1828201B1 (en) 2004-12-08 2005-12-08 Macrocyclic quinazoline derivatives and their use as mtki
ES05826362.5T ES2559305T3 (en) 2004-12-08 2005-12-08 Macrocyclic quinazoline derivatives and their use as MTKI
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BRPI0518394A BRPI0518394B8 (en) 2004-12-08 2005-12-08 compound derived from mtki quinazoline, use thereof, kinase inhibitor and pharmaceutical composition
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US11/720,693 US20100152174A1 (en) 2004-12-08 2005-12-08 Macrocyclic quinazole derivatives and their use as mtki
EA200701233A EA013995B1 (en) 2004-12-08 2005-12-08 Mtki quinazoline derivatives
NZ555496A NZ555496A (en) 2004-12-08 2005-12-08 Macrocyclic 4-amino-quinazoline derivatives and their use as MTKI
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IL183705A IL183705A (en) 2004-12-08 2007-06-06 Macrocyclic quinazole derivatives and their use as mtki
NO20073426A NO339891B1 (en) 2004-12-08 2007-07-05 MTKI quinazoline derivatives
HK08102117.6A HK1111416A1 (en) 2004-12-08 2008-02-26 Macrocyclic quinazoline derivatives and their use as mtki
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