WO2006059105A3 - Non-cytotoxic protein conjugates - Google Patents

Non-cytotoxic protein conjugates Download PDF

Info

Publication number
WO2006059105A3
WO2006059105A3 PCT/GB2005/004598 GB2005004598W WO2006059105A3 WO 2006059105 A3 WO2006059105 A3 WO 2006059105A3 GB 2005004598 W GB2005004598 W GB 2005004598W WO 2006059105 A3 WO2006059105 A3 WO 2006059105A3
Authority
WO
WIPO (PCT)
Prior art keywords
protease
sensory afferent
nociceptive sensory
afferent cell
fragment
Prior art date
Application number
PCT/GB2005/004598
Other languages
French (fr)
Other versions
WO2006059105A8 (en
WO2006059105A2 (en
Inventor
Keith Foster
John Chaddock
Charles Penn
K Roger Aoki
Joseph Francis
Lance Steward
Original Assignee
Health Prot Agency
Allergan Inc
Keith Foster
John Chaddock
Charles Penn
K Roger Aoki
Joseph Francis
Lance Steward
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0426394A external-priority patent/GB0426394D0/en
Priority to CA2588292A priority Critical patent/CA2588292C/en
Priority to AU2005311098A priority patent/AU2005311098B2/en
Priority to PL05810711T priority patent/PL1877073T3/en
Priority to DK05810711T priority patent/DK1877073T3/en
Priority to EP05810711.1A priority patent/EP1877073B1/en
Priority to ES05810711T priority patent/ES2431119T3/en
Priority to SI200531796T priority patent/SI1877073T1/en
Priority to US11/791,979 priority patent/US8187834B2/en
Application filed by Health Prot Agency, Allergan Inc, Keith Foster, John Chaddock, Charles Penn, K Roger Aoki, Joseph Francis, Lance Steward filed Critical Health Prot Agency
Priority to JP2007543908A priority patent/JP5174464B2/en
Publication of WO2006059105A2 publication Critical patent/WO2006059105A2/en
Publication of WO2006059105A3 publication Critical patent/WO2006059105A3/en
Priority to US11/829,118 priority patent/US7658933B2/en
Publication of WO2006059105A8 publication Critical patent/WO2006059105A8/en
Priority to US13/072,865 priority patent/US9139635B2/en
Priority to US13/212,868 priority patent/US8512984B2/en
Priority to US13/343,892 priority patent/US8603779B2/en
Priority to US13/418,453 priority patent/US8778634B2/en
Priority to US13/944,427 priority patent/US9012195B2/en
Priority to US14/300,746 priority patent/US9474807B2/en
Priority to US15/258,282 priority patent/US10619146B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/33—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Clostridium (G)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/04—Centrally acting analgesics, e.g. opioids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Pain & Pain Management (AREA)
  • Veterinary Medicine (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Public Health (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Neurosurgery (AREA)
  • Neurology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Rheumatology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

A non-cytotoxic protein conjugate for inhibition or reduction of exocytic fusion in a nociceptive sensory afferent cell, comprising: (i) a Targeting Moiety (TM), wherein said TM is an agonist of a receptor present on said nociceptive sensory afferent cell, and wherein said receptor undergoes endocytosis to be incorporated into an endosome within the nociceptive sensory afferent cell; (ii) a non-cytotoxic protease or a fragment thereof, wherein the protease or protease fragment is capable of cleaving a protein of the exocytic fusion apparatus of said nociceptive sensory afferent cell; and (iii) a Translocation Domain, wherein the Translocation Domain translocates the protease or protease fragment from within the endosome, across the endosomal membrane, and into the cytosol of the nociceptive sensory afferent cell. Nucleic acid sequences encoding the protein conjugates, methods of preparing same and uses thereof are also described.
PCT/GB2005/004598 2004-12-01 2005-12-01 Non-cytotoxic Protein Conjugates WO2006059105A2 (en)

Priority Applications (17)

Application Number Priority Date Filing Date Title
JP2007543908A JP5174464B2 (en) 2004-12-01 2005-12-01 Non-cytotoxic protein conjugate
AU2005311098A AU2005311098B2 (en) 2004-12-01 2005-12-01 Non-cytotoxic protein conjugates
PL05810711T PL1877073T3 (en) 2004-12-01 2005-12-01 Non-cytotoxic protein conjugates
DK05810711T DK1877073T3 (en) 2004-12-01 2005-12-01 Non-cytotoxic protein conjugates
EP05810711.1A EP1877073B1 (en) 2004-12-01 2005-12-01 Non-cytotoxic protein conjugates
CA2588292A CA2588292C (en) 2004-12-01 2005-12-01 Non-cytotoxic protein conjugates
ES05810711T ES2431119T3 (en) 2004-12-01 2005-12-01 Non-cytotoxic protein conjugates
SI200531796T SI1877073T1 (en) 2004-12-01 2005-12-01 Non-cytotoxic protein conjugates
US11/791,979 US8187834B2 (en) 2004-12-01 2005-12-01 Non-cytotoxic protein conjugates
US11/829,118 US7658933B2 (en) 2004-12-01 2007-07-27 Non-cytotoxic protein conjugates
US13/072,865 US9139635B2 (en) 2004-12-01 2011-03-28 Non-cytotoxic protein conjugates
US13/212,868 US8512984B2 (en) 2004-12-01 2011-08-18 Non-cytotoxic protein conjugates
US13/343,892 US8603779B2 (en) 2004-12-01 2012-01-05 Non-cytotoxic protein conjugates
US13/418,453 US8778634B2 (en) 2004-12-01 2012-03-13 Non-cytotoxic protein conjugates
US13/944,427 US9012195B2 (en) 2004-12-01 2013-07-17 Non-cytotoxic protein conjugates
US14/300,746 US9474807B2 (en) 2004-12-01 2014-06-10 Non-cytotoxic protein conjugates
US15/258,282 US10619146B2 (en) 2004-12-01 2016-09-07 Non-cytotoxic protein conjugates

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
GB0426394A GB0426394D0 (en) 2004-12-01 2004-12-01 Fusion proteins
GB0426394.3 2004-12-01
GB0504964A GB0504964D0 (en) 2004-12-01 2005-03-10 Fusion proteins
GB0504966.3 2005-03-10
GB0504966A GB0504966D0 (en) 2004-12-01 2005-03-10 Re-targeted toxin conjugates
GB0504964.8 2005-03-10

Related Child Applications (7)

Application Number Title Priority Date Filing Date
US11/791,979 A-371-Of-International US8187834B2 (en) 2004-12-01 2005-12-01 Non-cytotoxic protein conjugates
US11/829,118 Continuation US7658933B2 (en) 2004-12-01 2007-07-27 Non-cytotoxic protein conjugates
US13/072,865 Continuation US9139635B2 (en) 2004-12-01 2011-03-28 Non-cytotoxic protein conjugates
US13/212,868 Continuation-In-Part US8512984B2 (en) 2004-12-01 2011-08-18 Non-cytotoxic protein conjugates
US13/343,892 Continuation-In-Part US8603779B2 (en) 2004-12-01 2012-01-05 Non-cytotoxic protein conjugates
US13/418,453 Continuation-In-Part US8778634B2 (en) 2004-12-01 2012-03-13 Non-cytotoxic protein conjugates
US13/418,453 Continuation US8778634B2 (en) 2004-12-01 2012-03-13 Non-cytotoxic protein conjugates

Publications (3)

Publication Number Publication Date
WO2006059105A2 WO2006059105A2 (en) 2006-06-08
WO2006059105A3 true WO2006059105A3 (en) 2006-08-31
WO2006059105A8 WO2006059105A8 (en) 2007-11-08

Family

ID=35735383

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2005/004598 WO2006059105A2 (en) 2004-12-01 2005-12-01 Non-cytotoxic Protein Conjugates

Country Status (5)

Country Link
EP (1) EP1877073B1 (en)
AU (1) AU2005311098B2 (en)
CA (1) CA2588292C (en)
PL (1) PL1877073T3 (en)
WO (1) WO2006059105A2 (en)

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GB9617671D0 (en) * 1996-08-23 1996-10-02 Microbiological Res Authority Recombinant toxin fragments
US7192596B2 (en) 1996-08-23 2007-03-20 The Health Protection Agency Ipsen Limited Recombinant toxin fragments
EP2267010B1 (en) 1999-08-25 2014-05-07 Allergan, Inc. Activatable recombinant neurotoxins
US7138127B1 (en) 2000-01-19 2006-11-21 Allergan, Inc. Clostridial toxin derivatives and methods for treating pain
AU2005279741B2 (en) 2004-09-01 2011-10-06 Allergan, Inc. Degradable clostridial toxins
US8603779B2 (en) 2004-12-01 2013-12-10 Syntaxin, Ltd. Non-cytotoxic protein conjugates
US8399400B2 (en) 2004-12-01 2013-03-19 Syntaxin, Ltd. Fusion proteins
US8512984B2 (en) 2004-12-01 2013-08-20 Syntaxin, Ltd. Non-cytotoxic protein conjugates
GB0426397D0 (en) 2004-12-01 2005-01-05 Health Prot Agency Fusion proteins
GB0426394D0 (en) 2004-12-01 2005-01-05 Health Prot Agency Fusion proteins
US8778634B2 (en) 2004-12-01 2014-07-15 Syntaxin, Ltd. Non-cytotoxic protein conjugates
WO2006101809A1 (en) 2005-03-15 2006-09-28 Allergan, Inc. Modified clostridial toxins with enhanced targeting capabilities for endogenous clostridial toxin receptor systems
US8021859B2 (en) 2005-03-15 2011-09-20 Allergan, Inc. Modified clostridial toxins with altered targeting capabilities for clostridial toxin target cells
DK2154151T3 (en) 2005-09-19 2011-09-05 Allergan Inc Clostridium toxin inactivated clostridium toxins
AU2011202225B2 (en) * 2005-12-01 2015-08-20 Allergan, Inc. Non-cytotoxic protein conjugates
GB0610868D0 (en) * 2006-06-01 2006-07-12 Syntaxin Ltd Treatment of pain
GB0610867D0 (en) 2006-06-01 2006-07-12 Syntaxin Ltd Treatment of pain
EP2038298A2 (en) 2006-07-11 2009-03-25 Allergan, Inc. Modified clostridial toxins with enhanced translocation capabilities and altered targeting activity for clostridial toxin target cells
AU2007347781B2 (en) 2006-07-11 2013-10-03 Allergan, Inc. Modified clostridial toxins with enhanced translocation capability and enhanced targeting activity
CN101918021A (en) 2007-10-23 2010-12-15 阿勒根公司 Methods of treating urogenital-neurological disorders using modified clostridial toxins
JP5514399B2 (en) * 2007-11-02 2014-06-04 国立大学法人 岡山大学 Method for producing inorganic sulfur compound hydrolase
GB0725321D0 (en) * 2007-12-31 2008-02-06 Syntaxin Ltd Delivery vehicles
CA2746425C (en) 2008-12-10 2016-05-03 Allergan, Inc. Clostridial toxin pharmaceutical compositions
EP2406629B9 (en) 2009-03-13 2014-03-26 Allergan, Inc. Immuno-based retargeted endopeptidase activity assays
US20100303757A1 (en) 2009-05-29 2010-12-02 Allergan, Inc. Methods of Treating Chronic Neurogenic Inflammation Using Interleukin Retargeted Endopepidases
US20100303794A1 (en) 2009-05-29 2010-12-02 Allergan, Inc. Methods of Treating Urogenital-Neurological Disorders Using Glucagon Like Hormone Retargeted Endopepidases
SG10201406930UA (en) 2009-07-27 2014-11-27 Nocicepta Llc Methods For Treatment Of Pain
US20110064713A1 (en) * 2009-08-14 2011-03-17 Allergan, Inc. Methods of Treating Cancer Using Opioid Retargeted Endopepidases
AU2010282274A1 (en) 2009-08-14 2012-03-15 Allergan, Inc. Methods of treating cancer using growth factor retargeted endopeptidases
EP2464364A2 (en) 2009-08-14 2012-06-20 Allergan, Inc. Methods of treating cancer using galanin retargeted endpeptidases
US20110070215A1 (en) 2009-08-14 2011-03-24 Allergan, Inc. Methods of treating cancer using neurotrophin retargeted endopeptidases
CA2771298A1 (en) 2009-08-14 2011-02-17 Allergan, Inc. Methods of treating cancer using glucagon-like hormone retargeted endopeptidases
EP2464365A2 (en) 2009-08-14 2012-06-20 Allergan, Inc. Methods of treating cancer using tachykinin retargeted endopeptidases
US20120207704A1 (en) 2011-02-14 2012-08-16 Allergan, Inc. Inhibiting Aberrant Blood Vessel Formation Using Growth Factor Retargeted Endopeptidases
WO2012112426A1 (en) 2011-02-14 2012-08-23 Allergan, Inc. Arginine vasopressin retargeted clostridial endopeptidases for use in treating benign prostatic hyperplasia
WO2012112434A1 (en) 2011-02-14 2012-08-23 Allergan, Inc. Inhibiting aberrant blood vessel formation using retargeted endopeptidases
WO2012112432A1 (en) 2011-02-14 2012-08-23 Allergan, Inc. Methods of inhibiting aberrant blood vessel formation using opioid retargeted endopeptidases
US9803004B2 (en) 2011-11-11 2017-10-31 Ucb Biopharma Sprl Albumin binding antibodies and binding fragments thereof
US20140056870A1 (en) 2012-08-27 2014-02-27 Allergan, Inc. Fusion proteins
SI3242676T1 (en) 2015-01-07 2024-02-29 Tonix Pharma Limited Magnesium-containing oxytocin formulations and methods of use
MX2019002835A (en) 2016-09-13 2019-09-04 Allergan Inc Stabilized non-protein clostridial toxin compositions.
TW201814045A (en) 2016-09-16 2018-04-16 英商艾普森生物製藥有限公司 Method for producing di-chain clostridial neurotoxins
JP7118055B2 (en) 2016-09-29 2022-08-15 イプセン バイオファーム リミテッド hybrid neurotoxin
EP3312290A1 (en) 2016-10-18 2018-04-25 Ipsen Biopharm Limited Cellular vamp cleavage assay
GB202007441D0 (en) 2020-05-19 2020-07-01 Autolus Ltd Polypeptide
EP3915580A1 (en) 2020-05-29 2021-12-01 Numab Therapeutics AG Multispecific antibody
EP3988568A1 (en) 2020-10-21 2022-04-27 Numab Therapeutics AG Combination treatment
IL303295A (en) 2020-12-07 2023-07-01 UCB Biopharma SRL Multi-specific antibodies and antibody combinations
CA3208781A1 (en) 2021-02-02 2022-08-11 Bithi CHATTERJEE Multispecific antibodies having specificity for ror1 and cd3
EP4183800A1 (en) 2021-11-19 2023-05-24 Medizinische Hochschule Hannover Novel sars-cov-2 neutralizing antibodies

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US5989545A (en) * 1995-04-21 1999-11-23 The Speywood Laboratory Ltd. Clostridial toxin derivatives able to modify peripheral sensory afferent functions
WO2004024909A2 (en) * 2002-09-12 2004-03-25 Health Protection Agency Recombinant colstridium neurotoxin fragments
EP1422240A2 (en) * 2002-09-24 2004-05-26 ITALFARMACO S.p.A. Analogs of nociceptin
WO2005023309A2 (en) * 2003-09-11 2005-03-17 Health Protection Agency Design of re-targeted toxin conjugates

Family Cites Families (1)

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WO2003093293A2 (en) * 2002-04-29 2003-11-13 Saint Louis University Peptide antagonists of tgf-beta family members and therapeutic uses thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5989545A (en) * 1995-04-21 1999-11-23 The Speywood Laboratory Ltd. Clostridial toxin derivatives able to modify peripheral sensory afferent functions
WO2004024909A2 (en) * 2002-09-12 2004-03-25 Health Protection Agency Recombinant colstridium neurotoxin fragments
EP1422240A2 (en) * 2002-09-24 2004-05-26 ITALFARMACO S.p.A. Analogs of nociceptin
WO2005023309A2 (en) * 2003-09-11 2005-03-17 Health Protection Agency Design of re-targeted toxin conjugates

Also Published As

Publication number Publication date
EP1877073A2 (en) 2008-01-16
AU2005311098A1 (en) 2006-06-08
CA2588292C (en) 2019-01-15
EP1877073B1 (en) 2013-09-25
WO2006059105A8 (en) 2007-11-08
AU2005311098B2 (en) 2011-08-11
CA2588292A1 (en) 2006-06-08
PL1877073T3 (en) 2014-03-31
WO2006059105A2 (en) 2006-06-08

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