WO2006052351A3 - Biological microbeads for various flow cytometric applications - Google Patents

Biological microbeads for various flow cytometric applications Download PDF

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Publication number
WO2006052351A3
WO2006052351A3 PCT/US2005/036038 US2005036038W WO2006052351A3 WO 2006052351 A3 WO2006052351 A3 WO 2006052351A3 US 2005036038 W US2005036038 W US 2005036038W WO 2006052351 A3 WO2006052351 A3 WO 2006052351A3
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WO
WIPO (PCT)
Prior art keywords
microbeads
biological
cytometric applications
detection
biological microbeads
Prior art date
Application number
PCT/US2005/036038
Other languages
French (fr)
Other versions
WO2006052351A2 (en
Inventor
Gabor Szabo
Gyoergy Lustyik
Miklos Szabo
Original Assignee
Cedars Sinai Medical Center
Gabor Szabo
Gyoergy Lustyik
Miklos Szabo
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cedars Sinai Medical Center, Gabor Szabo, Gyoergy Lustyik, Miklos Szabo filed Critical Cedars Sinai Medical Center
Priority to US11/575,859 priority Critical patent/US20080020382A1/en
Priority to EP05806051A priority patent/EP1797436A2/en
Publication of WO2006052351A2 publication Critical patent/WO2006052351A2/en
Publication of WO2006052351A3 publication Critical patent/WO2006052351A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/554—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being a biological cell or cell fragment, e.g. bacteria, yeast cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Microbiology (AREA)
  • Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The invention relates to biological microbeads, and in certain embodiments, to the use thereof in flow-cytometric applications. Biological microbeads are fixed cells that may be surface-modified with proteins or other molecules that have specific binding properties. These surface-bound proteins may bind to target compounds bearing fluorescent labels that facilitate detection by flow cytometry. Provided are compositions and methods for the use of biological microbeads as an inexpensive alternative to synthetic microbeads for in an extensive number of flow-cytometric applications, including quantitative PCR, detection of nucleases, detection of proteases, and immunoassays.
PCT/US2005/036038 2004-10-08 2005-10-07 Biological microbeads for various flow cytometric applications WO2006052351A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US11/575,859 US20080020382A1 (en) 2004-10-08 2005-10-07 Biological Microbeads for Various Flow Cytometric Applications
EP05806051A EP1797436A2 (en) 2004-10-08 2005-10-07 Biological microbeads for various flow cytometric applications

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US61699704P 2004-10-08 2004-10-08
US60/616,997 2004-10-08

Publications (2)

Publication Number Publication Date
WO2006052351A2 WO2006052351A2 (en) 2006-05-18
WO2006052351A3 true WO2006052351A3 (en) 2006-09-28

Family

ID=36336924

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/036038 WO2006052351A2 (en) 2004-10-08 2005-10-07 Biological microbeads for various flow cytometric applications

Country Status (3)

Country Link
US (1) US20080020382A1 (en)
EP (1) EP1797436A2 (en)
WO (1) WO2006052351A2 (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003012449A2 (en) * 2001-07-27 2003-02-13 Lonza Group Ag Method for selecting antibody expressing cells
US20040137532A1 (en) * 2001-03-02 2004-07-15 Gabor Szabo Antibody competition test (act) for the qualitative and conformation dependent quantitative detection of the conformation changes of p-glycoprotein and other cell surface receptors, pumps and proteins, and uses thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040137532A1 (en) * 2001-03-02 2004-07-15 Gabor Szabo Antibody competition test (act) for the qualitative and conformation dependent quantitative detection of the conformation changes of p-glycoprotein and other cell surface receptors, pumps and proteins, and uses thereof
WO2003012449A2 (en) * 2001-07-27 2003-02-13 Lonza Group Ag Method for selecting antibody expressing cells

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
NOSEK J. ET AL.: "NADH dehydrogenase subunit genes in the mitochondrial DNA of yeasts", J. BACTERIOL., vol. 176, no. 18, September 1994 (1994-09-01), pages 5622 - 5630 *
PATAKI J. ET AL.: "Biological Microbeads for Flow-Cytometric Immunoassays, Enzyme Titrations, and Quantitative PCR", CYTOMETRY PART A, vol. 68A, 2005, pages 45 - 52 *
SHANTZ E.M. ET AL.: "PANSORBIN Staphylococcus aureus cells: A Review and Bibliography of the Immunological Applications of Fixed Protein A-Bearing Staphylococcus aureus Cells", CALBIOCHEM-NOVABIOCHEM CORPORATION, 2000, pages 1 - 36 *
SZABO G. ET AL.: "Fixed cells as microbeads for various applications", CYTOMETRY, vol. 59A, no. 1, May 2004 (2004-05-01), pages 154 *
SZABO G. ET AL.: "Fluorescent Staphylococci as Microbeads", CYTOMETRY, vol. 10, 1989, pages 801 - 802 *

Also Published As

Publication number Publication date
WO2006052351A2 (en) 2006-05-18
US20080020382A1 (en) 2008-01-24
EP1797436A2 (en) 2007-06-20

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