WO2006047912A1

WO2006047912A1 - A centrifugal tube for cleaning microarray chips

Info

Publication number: WO2006047912A1
Application number: PCT/CN2004/001342
Authority: WO
Inventors: Feijun Xian; Yimin Sun; Xiaoxiang Zhu; Wanli Xing; Yan Wang; Jing Cheng
Original assignee: Capitalbio Corporation; Tsinghua University
Priority date: 2004-11-05
Filing date: 2004-11-23
Publication date: 2006-05-11
Also published as: CN2773687Y

Abstract

A centrifugal tube for cleaning microarray chips includes a tube body. A couple of slots are mounted on the inner wall of such tube body for positioning chips. At least a ridge on which protrudes from the inner surface in order to support chips is set inside the bottom of such tube body.

Description

Microarray chip cleaning, centrifuge tube

The invention relates to a microarray chip cleaning and a centrifuge tube.

Background technique

Microarray Chips are widely used in biological, medical, food safety and related fields as a fast and efficient inspection tool. Microarray chips are used to place and fix a variety of probes (such as nucleic acids, proteins, or tissues) in a regular row and column arrangement. The current substrate is mostly standard slides (size is about 25). Mmx75mmx'l mm). The basic steps for detection and analysis using microarray chips include: sample preparation, biochemical reactions, and results detection. In the process of biochemical reaction of the microarray chip, chip cleaning and centrifugation are often required.

At present, the cleaning method of the microarray chip is generally carried out in a cleaning container (such as a common plastic box, a beaker) on a shaker. The specific operation process is as follows: Place the chip in a cleaning container containing the cleaning liquid, place the cleaning container on the shaker, turn on the power of the shaker for oscillation cleaning, and remove the chip from the cleaning container after the cleaning is completed, and perform centrifugation.

The commonly used microarray chip centrifugation methods mainly have the following two methods: First, the cleaned chip (standard slide) is inserted into a 50 ml ordinary plastic centrifuge tube and centrifuged on a conventional centrifuge; Second, the application of TeleChem (Http: //www.array it com) Developed a microarray chip centrifuge for centrifugation of a standard slide. The cleaned slides are inserted into the slide holder on the centrifuge and centrifuged.

For the first method, when these 50ml centrifuge tubes are used for centrifugation of the chip, the following problems exist: 1) The 50 ml centrifuge tube is generally long and has a certain taper (the smaller the cross-sectional diameter of the tube toward the bottom), causing chip centrifugation. After the card is stuck at the bottom of the centrifuge tube, it is not easy to take out; 2) When the chip width tolerance is large (that is, the width is wide), the centrifuge tube is often not inserted smoothly; 3) The cleaning and centrifugation of the chip are completed in different containers, the whole process The need to manually transfer the chip is not only cumbersome to operate, but also has security risks during the transfer process.

For the second method, a special centrifuge is required, which is costly; and the centrifugal efficiency is low, and only one chip can be centrifuged at a time; at the same time, the cleaning and centrifugation of the chip are also completed in different containers, and the whole process requires manual transfer of the chip. There are also problems such as cumbersome operations.

Invention disclosure The object of the present invention is a microarray chip cleaning and centrifuge tube capable of both cleaning and centrifuging functions. In order to achieve the above object, the present invention uses the following technical solution: a microarray chip cleaning, a centrifuge tube, comprising a tube body, the inner side wall of the tube body is provided with a pair of chip positioning grooves, the tube of the tube body The bottom inner side is provided with at least one chip support rib protruding from the inner surface.

In order to facilitate the pick-and-place chip, the portion of the chip positioning groove near the nozzle is configured as a bell mouth. In order to ensure the watertightness of the chip when used for cleaning, and the hermeticity when used for storing the chip, the microarray chip cleaning and centrifuge tube further includes a tube cover, the tube cover and the tube body Connected by thread. A sealing ring is also disposed in the tube cover.

As an improvement of the present invention, the longitudinal section of the tubular body near the bottom portion of the tube is trapezoidal, forming a tapered liquid collecting space, which ensures the centrifugal effect of the chip.

In the present invention, the tubular body surface of the tubular body is a flat surface or a curved surface; and the tubular body is preferably cylindrical.

In order to prevent adhesion of biological small molecules, the inner wall of the tube body is a smooth surface, a calender surface or a sand surface; wherein, in the light surface, an auroral surface is preferred.

In order to facilitate the cleaning of the chip, a fixed volume of cleaning liquid is added, and the outer wall of the tube body is further provided with a volume scale mark.

In use, put the chip in the tube, add the cleaning solution, screw in the tube cap with the sealing ring, put it on the horizontal shaker for cleaning, pour off the cleaning solution after cleaning, and then centrifuge with a conventional centrifuge to complete Chip cleaning, centrifugation.

The invention has the following advantages due to adopting the above technical solutions: 1) chip support ribs and chip sets

The setting of the position groove and the chip guiding groove facilitates the loading and unloading operation of the chip, and the position of the chip is unchanged in the tube before and after centrifugation, thereby avoiding the phenomenon of "sinking" of the chip when the ordinary 50 ml centrifuge tube is used for centrifugation. Cleaning and centrifuging in one tube; 2) The setting of the tube cover and the sealing ring makes the invention have good sealing performance, which can be used not only for chip cleaning, centrifugation, but also for chip storage; 3) The invention can be operated by a conventional centrifuge without using a dedicated centrifugal device and is easy to operate.

DRAWINGS

1A is a schematic structural view of the present invention;

Figure 1B is a schematic view showing the internal structure of the present invention;

Figure 1C is a cross-sectional view taken along line A-A of Figure 1A; 2A is a schematic structural view of the present invention after loading a chip;

Figure 2B is a cross-sectional view taken along line B-B of Figure 2A;

Figure 3A is a schematic view of the structure of the present invention with a tube cover and a sealing ring;

Figure 3B is a cross-sectional view taken along line C-C of Figure 3A;

4A is a schematic structural view of a cleaning tube having a cylindrical body;

4B is a schematic view showing the internal structure of a cleaning tube having a cylindrical body;

Figure 5A is a result scan of a chip using conventional cleaning and centrifugation;

5B is a result scan of a chip for performing chip cleaning and centrifugation using the present invention; FIG. 5C is a comparison diagram of signal values of scan results;

Figure 5D is a comparison of the coefficient of variation values.

The best way to implement the invention

Example 1. Microarray chip cleaning, centrifuge tube

1A, FIG. 1B and FIG. 1C, in the embodiment, the microarray chip cleaning and the centrifuge tube comprise a tube body 1. The inner wall of the tube bottom is provided with a chip supporting rib 2, and the side wall of the tube body has a chip positioning groove 3, the chip The support rib 2 and the chip positioning groove 3 are arranged to ensure that the position of the chip is unchanged in the tube before and after centrifugation, thereby avoiding the phenomenon of "sinking" of the chip when the ordinary 50 ml centrifuge tube is used for centrifugation; The side wall of the nozzle also has a chip guiding groove 4 with a certain curvature. The chip guiding groove 4 has a "horn" shape and is integrated with the chip positioning groove 3; in order to ensure the centrifugal effect of the chip, the tube body is close to The longitudinal section of the bottom portion of the tube is trapezoidal, and a liquid collecting space 5 having a certain taper is formed at the bottom of the tube.

A volume scale mark 6 is arranged on the outer wall of the pipe body 1 to facilitate adding a certain volume of cleaning liquid when cleaning the chip; the shape of the surface 8 of the pipe body and the lower surface 9 of the pipe body may be a flat shape, which is convenient for cleaning the pipe. It can also be used to mark and paste labels. The surface of the inner wall of the pipe body 1 may be an auroral surface, a glossy surface, a calender surface, or a sand surface, etc. In order to prevent adhesion of biological small molecules, the surface of the inner wall is preferably an auroral surface.

The material of the tube body 1 may be polypropylene, polycarbonate, plexiglass, polystyrene, ABS, polyethylene, polyvinyl chloride, polyoxymethylene, etc., and the light transmittance may be opaque, translucent, or transparent, and加工Processing by conventional injection molding or blow molding processes.

2A and 2B are schematic views showing the structure of the cleaning and centrifugation tube loading chip 10. The chip 10 is placed in the chip positioning groove 3, and one end thereof is in contact with the chip supporting rib 2. The cleaning tube is not only It can be used to load ordinary chips, and can load multi-sample fence type chips, which can be centrifuged on a conventional centrifuge.

3A and 3B are schematic views showing the structure of the cleaning tube of the present invention equipped with a tube cover and a sealing ring. In order to ensure the watertightness when cleaning the chip 10 and the hermeticity of the memory chip 10, the present invention may also be provided with a tube cover 11 and a sealing ring 12, and the tube cover 11 and the tube body 1 are connected by a thread 13, and the sealing ring 12 is mounted in the tube cover 11.

The material of the tube cover 11 can be the same as that of the tube body 1, such as polypropylene, polycarbonate, plexiglass, polystyrene, ABS, polyethylene, polyvinyl chloride, polyoxymethylene, etc., and the light transmittance can be opaque. , translucent, or transparent, can be processed by conventional injection molding or blow molding processes.

The sealing ring 12 can be processed by using silicone rubber, natural rubber, nitrile rubber, urethane rubber, neoprene rubber, fluororubber rubber, ethylene propylene rubber or the like. The common processing techniques include vulcanization or stamping, and the light transmittance is opaque. Translucent, 'or transparent, etc.

4A and 4B are schematic views showing the structure of a cleaning tube having a cylindrical body. The tube body surface 8 of the tube has a cylindrical surface shape similar to the outer surface of the 50 ml centrifuge tube.

Example 2, the application of the invention in the field of protein chip detection

The detection of veterinary drug residues (enrofloxacin, neomycin, sulfonamide, streptomycin) in animal-derived foods using microarray protein chips based on standard slides, chip fabrication, detection, etc. Conventional methods are performed in the art. During the test, the same chip format (10-hole fence chip and fence chip cover), the same sample, the same probe, the same immune reaction conditions, and the same scanning parameters were used. Two different cleaning and centrifugation methods:

1) Conventional chip cleaning and centrifugation method: place the chip in a common plastic box containing the cleaning solution, place the plastic box on a horizontal shaker for cleaning, remove the chip from the plastic box after cleaning, and put it in 50ral cleaning Tube, centrifuge with a conventional centrifuge (flying pigeon TDL-5).

2) The method of chip cleaning and centrifugation by using the invention: placing the chip on the microarray chip cleaning tube body, adding the cleaning liquid, screwing into the tube cover with the sealing ring, and placing it on the horizontal shaker for cleaning, after the cleaning is completed Pour off the cleaning solution and centrifuge with a conventional centrifuge (flying pigeon TDL-5).

5A is a result scan of a chip using conventional cleaning and centrifugation, FIG. 5B is a result scan of a chip for performing chip cleaning and centrifugation using the present invention, FIG. 5C is a comparison chart of signal values of the scan result, and FIG. 5D is a coefficient of variation value. Comparison chart. As can be seen from the figure, with conventional chip cleaning, centrifugation Compared with the method, the difference between the probe signal intensity value and the coefficient of variation of the chip after cleaning and centrifugation according to the present invention is within an acceptable error range, indicating that the present invention can be used for cleaning, centrifuging, and microchip chip. It is very convenient to pick and place from the cleaning tube.

Industrial application

Microarray chip cleaning and centrifugal tube structure are simple, convenient and practical, and will be widely used in cleaning and centrifugation of microarray chips.

Claims

Extremely demanding book

A microarray chip cleaning and centrifuge tube comprising a tube body, wherein: the inner side wall of the tube body is provided with a pair of chip positioning grooves, and the inner side of the tube body of the tube body is provided with at least one protrusion Chip support ribs on the inner surface.

2. The microarray chip cleaning and centrifuge tube according to claim 1, wherein: the portion of the chip positioning groove adjacent to the nozzle is flared.

The microarray cleaning and centrifugation tube according to claim 1 or 2, wherein: the microarray chip cleaning and the centrifuge tube further comprise a tube cover, and the tube cover is screwed to the tube body. .

4. The microarray chip cleaning and centrifuge tube according to claim 3, wherein: the tube cover is further provided with a sealing ring.

The microarray chip cleaning and centrifuge tube according to claim 1 or 2, wherein the longitudinal section of the tube body near the bottom portion of the tube is trapezoidal.

6. The microarray chip cleaning and centrifuge tube according to claim 5, wherein: the surface of the tube body is a flat surface or a curved surface.

7. The microarray chip cleaning and centrifuge tube according to claim 6, wherein: the tube body has a cylindrical shape.

The microarray cleaning and centrifuge tube according to claim 1 or 2, wherein the inner wall of the tube body is a smooth surface, a calender surface or a sand surface.

The microarray cleaning and centrifugation tube according to claim 1 or 2, wherein the outer wall of the tube body is further provided with a volume scale mark.