WO2006043095A3 - Monitoring enzyme-substrate reactions - Google Patents

Monitoring enzyme-substrate reactions Download PDF

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Publication number
WO2006043095A3
WO2006043095A3 PCT/GB2005/004089 GB2005004089W WO2006043095A3 WO 2006043095 A3 WO2006043095 A3 WO 2006043095A3 GB 2005004089 W GB2005004089 W GB 2005004089W WO 2006043095 A3 WO2006043095 A3 WO 2006043095A3
Authority
WO
WIPO (PCT)
Prior art keywords
enzyme
reactions
redox
substrate
methods
Prior art date
Application number
PCT/GB2005/004089
Other languages
French (fr)
Other versions
WO2006043095A8 (en
WO2006043095A2 (en
Inventor
Richard Gilbert
Original Assignee
E2V Biosensors Ltd
Richard Gilbert
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by E2V Biosensors Ltd, Richard Gilbert filed Critical E2V Biosensors Ltd
Priority to JP2007537392A priority Critical patent/JP2008517593A/en
Priority to AU2005297094A priority patent/AU2005297094A1/en
Priority to US11/665,650 priority patent/US20080160550A1/en
Priority to EP05796141A priority patent/EP1805321A2/en
Publication of WO2006043095A2 publication Critical patent/WO2006043095A2/en
Publication of WO2006043095A3 publication Critical patent/WO2006043095A3/en
Publication of WO2006043095A8 publication Critical patent/WO2006043095A8/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/28—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving peroxidase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/30—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving catalase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/42—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/485—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Electrochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Methods of electrochemically monitoring enzyme-substrate reactions are described. The substrate is tagged with a redox-active group. Modification of the substrate by an enzyme can then be monitored electrochemically by means of the redox-active group. Such methods can be used in particular to monitor non-redox enzyme reactions, such as kinase, phosphatase, or protease reactions, although redox enzyme reactions can also be monitored by such methods. Screening assays to identify modulators of enzyme activity or new enzyme substrates are also described.
PCT/GB2005/004089 2004-10-22 2005-10-21 Monitoring enzyme-substrate reactions WO2006043095A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2007537392A JP2008517593A (en) 2004-10-22 2005-10-21 Monitoring enzyme-substrate reactions
AU2005297094A AU2005297094A1 (en) 2004-10-22 2005-10-21 Monitoring enzyme-substrate reactions
US11/665,650 US20080160550A1 (en) 2004-10-22 2005-10-21 Monitoring Enzyme-Substrate Reactions
EP05796141A EP1805321A2 (en) 2004-10-22 2005-10-21 Monitoring enzyme-substrate reactions

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0423561A GB2419880B (en) 2004-10-22 2004-10-22 Monitoring enzyme-substrate reactions
GB0423561.0 2004-10-22

Publications (3)

Publication Number Publication Date
WO2006043095A2 WO2006043095A2 (en) 2006-04-27
WO2006043095A3 true WO2006043095A3 (en) 2006-07-06
WO2006043095A8 WO2006043095A8 (en) 2006-07-27

Family

ID=33485082

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2005/004089 WO2006043095A2 (en) 2004-10-22 2005-10-21 Monitoring enzyme-substrate reactions

Country Status (8)

Country Link
US (1) US20080160550A1 (en)
EP (1) EP1805321A2 (en)
JP (1) JP2008517593A (en)
CN (1) CN101061233A (en)
AU (1) AU2005297094A1 (en)
GB (1) GB2419880B (en)
HK (1) HK1090955A1 (en)
WO (1) WO2006043095A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102703574B (en) * 2012-06-08 2015-05-13 厦门大学 Method for screening coenzyme dependant type oxidation-reduction enzyme

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0125139A2 (en) * 1983-05-05 1984-11-14 MediSense, Inc. Assay techniques utilising specific binding agents
GB2173313A (en) * 1985-02-21 1986-10-08 Genetics Int Inc Electrochemical detection of redox species produced or released from macromolecules
WO1999064847A1 (en) * 1998-06-08 1999-12-16 Xanthon, Inc. Electrochemical probes for detection of molecular interactions and drug discovery
US6110696A (en) * 1993-08-27 2000-08-29 Roche Diagnostics Corporation Electrochemical enzyme assay
DE19917052A1 (en) * 1999-04-15 2000-10-19 Wolfgang Schuhmann Chemical or biochemical assay, involving electrochemically assessing a change in diffusion coefficient of redox-active species due to a specific binding reaction, useful e.g. for determining antigens or DNA fragments

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2455083A1 (en) * 1979-04-24 1980-11-21 Jozefonvicz Marcel Electrochemical protease or anti-protease determn. - by amperometric determn. of amine released from peptide amide substrate
EP0018002B1 (en) * 1979-04-24 1983-02-09 Marcel Jozefonvicz Process for the determination of proteases and antiproteases
JP3468312B2 (en) * 1994-07-29 2003-11-17 株式会社三菱化学ヤトロン Method for detecting alkaline phosphatase
EP1157029B1 (en) * 1999-02-23 2005-12-28 Pentapharm AG Oligopeptide derivatives for the electrochemical measurement of protease activity
GB0205455D0 (en) * 2002-03-07 2002-04-24 Molecular Sensing Plc Nucleic acid probes, their synthesis and use
GB0316075D0 (en) * 2003-07-09 2003-08-13 Molecular Sensing Plc Protease detection assay

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0125139A2 (en) * 1983-05-05 1984-11-14 MediSense, Inc. Assay techniques utilising specific binding agents
GB2173313A (en) * 1985-02-21 1986-10-08 Genetics Int Inc Electrochemical detection of redox species produced or released from macromolecules
US6110696A (en) * 1993-08-27 2000-08-29 Roche Diagnostics Corporation Electrochemical enzyme assay
US20030017519A1 (en) * 1993-08-27 2003-01-23 Brown Mary E. Electrochemical enzyme assay
WO1999064847A1 (en) * 1998-06-08 1999-12-16 Xanthon, Inc. Electrochemical probes for detection of molecular interactions and drug discovery
DE19917052A1 (en) * 1999-04-15 2000-10-19 Wolfgang Schuhmann Chemical or biochemical assay, involving electrochemically assessing a change in diffusion coefficient of redox-active species due to a specific binding reaction, useful e.g. for determining antigens or DNA fragments

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
PAULINE M. ALLEN, H. ALLEN O. HILL, NICHOLAS J. WALTON: "Surface Modifiers for the promotion of Direct electrochemistry of cytochrome c", JOURNAL OF ELECTROANALYTICAL CHEMISTRY, vol. 178, 1984, pages 69 - 86, XP002377314 *

Also Published As

Publication number Publication date
GB0423561D0 (en) 2004-11-24
GB2419880B (en) 2008-04-30
US20080160550A1 (en) 2008-07-03
HK1090955A1 (en) 2007-01-05
JP2008517593A (en) 2008-05-29
AU2005297094A1 (en) 2006-04-27
WO2006043095A8 (en) 2006-07-27
CN101061233A (en) 2007-10-24
WO2006043095A2 (en) 2006-04-27
GB2419880A (en) 2006-05-10
EP1805321A2 (en) 2007-07-11

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