WO2006033429A1 - 細胞凍結保存用組成物 - Google Patents
細胞凍結保存用組成物 Download PDFInfo
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- WO2006033429A1 WO2006033429A1 PCT/JP2005/017577 JP2005017577W WO2006033429A1 WO 2006033429 A1 WO2006033429 A1 WO 2006033429A1 JP 2005017577 W JP2005017577 W JP 2005017577W WO 2006033429 A1 WO2006033429 A1 WO 2006033429A1
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- WIPO (PCT)
- Prior art keywords
- cell
- composition
- sericin
- cryopreservation
- weight
- Prior art date
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
- A01N1/0221—Freeze-process protecting agents, i.e. substances protecting cells from effects of the physical process, e.g. cryoprotectants, osmolarity regulators like oncotic agents
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
- C07K14/43563—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects
- C07K14/43586—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects from silkworms
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/04—Preserving or maintaining viable microorganisms
Definitions
- the present invention relates to a composition for cell cryopreservation that can provide good cell viability.
- the cryopreservation of cultured cells is carried out for the purpose of preventing cell alteration due to passage, preventing contamination with bacteria accompanying passage, and eliminating the complexity of passage maintenance.
- the cryopreservation technique for animal cells has been to suspend the cells in a culture solution containing 5-15% dimethyl sulfoxide (DMSO) or glycerin, or bovine serum, and place this in a freezing tube or Generally, a method of sealing in ampules, cooling at a rate of about 1 ° CZ using a program freezer, and finally storing in liquid nitrogen (196 ° C) was performed.
- cryopreservation solution that does not require the use of a program freezer, for example, in Japanese Patent Publication No. 6-46840, a temperature of 80 ° C is directly applied from ice without the necessity of using a program freezer. Cryopreservation solutions that can be transferred have been proposed. However, this cryopreservation solution contained serum components.
- cryoprotective effect of these components alone is not sufficient compared with the serum components as far as the present inventors know.
- the present inventors now use a silk protein, sericin, in combination with an amino acid and Z or a saccharide, without using a serum-derived component, which is equivalent to using a serum-derived component. It has been found that an excellent cell cryoprotective effect can be exhibited. This effect is
- an object of the present invention is to provide a cell cryopreservation solution capable of freezing cells for a long period of time without using serum and serum-derived components.
- the composition for cell cryopreservation according to the present invention comprises at least sericin and one or more components selected from the group consisting of amino acids and sugars.
- the cell cryopreservation method according to the present invention comprises placing the target cell in the cell cryopreservation composition and cryopreserving it.
- the composition for cell cryopreservation according to the present invention exhibits an excellent anti-freezing effect as compared to the case of sericin alone, and also has the same strength as that of the case where a serum-derived component is used. Or it shows the cryoprotective effect beyond it.
- the composition for cell cryopreservation of the present invention does not contain serum or serum-derived components, there is little possibility of changing the properties of stored cells and there is almost no risk of contamination with unknown viruses. . Since the composition of the present invention has a clear composition, it is advantageous in terms of maintaining a stable quality with high safety and a stable supply.
- the cells can be frozen in an 80 ° C freezer directly without using a program freezer after filling the cells in a freezing tube or ampoule. Compared with, operation during freezing can be simplified.
- target cells can be stored frozen for a long period of time, and a high cell survival rate can be achieved even after cryopreservation. Thereby, the time required to grow to the target number of cells can be shortened.
- the cell cryopreservation composition according to the present invention comprises at least sericin and at least one component selected from the group consisting of amino acids and sugars. Therefore, as long as the above components are included, the composition according to the present invention may further include other cell cryoprotective components, buffer components, and other optional components.
- the composition for cell cryopreservation according to the present invention is usually used as a cryopreservation solution in an aqueous solution state by adding water. Therefore, the composition for cell cryopreservation here includes not only a solid state but also a liquid state.
- cell cryopreservation refers to freezing and storing cells for the purpose of maintaining the cells for a long period of time without being subcultured
- the freezing method and the storage method are not particularly limited. I can't. Therefore, for example, in addition to the case where cells are directly put into a freezer and frozen and stored without using a program freezer, the case where cells are frozen and stored using a program freezer is also included.
- the "cell” is not particularly limited as long as it is a cell that may be subjected to cryopreservation, and may be any of microorganisms, bacteria, animal cells, and plant cells.
- the “cell” is preferably an animal cell.
- the term “animal” as used herein includes mammals including humans, fish, birds, insects and the like.
- “animal cells” include cells established as cultured cells, established cells obtained from biological tissue strength, normal cells, transformed cells obtained by genetic engineering techniques, and the like. It ’s in the form of a gap.
- sericin is derived from a natural product, even if it is derived from natural products.
- sericin produced by rabbits, wild rabbits and the like can be used as sericin.
- sericin is highly purified and substantially free of impurities.
- Sericin in the present invention includes sericin and a hydrolyzate thereof.
- the sericin hydrolyzate can be obtained by subjecting a non-hydrolyzed sericin to hydrolysis using conventional means such as an alkali or an enzyme.
- sericin can be obtained by extracting cocoons or raw silk isotropic force by a conventional extraction method.
- sericin can be obtained by using, for example, silkworm, raw silk, or silk fabric as a raw material, and hydrolyzing and extracting it with hot water, acid, alkali, enzyme, or the like.
- the obtained sericin is purified to a high purity (for example, a total nitrogen amount of 13% or more of sericin solid) by the method described in Japanese Patent No. 30 11759 as necessary, and prepared in a solid form such as a powder. Is done.
- this solid form of sericin can be blended as it is in the cell cryopreservation composition, but if necessary, a solution in which the solid form of sericin is dissolved or suspended in water or a buffer solution. State And may be blended into a composition for cell cryopreservation.
- the average molecular weight of sericin is not particularly limited, but is preferably from 3,000 to 300,000, more preferably ⁇ is 5,000 to wind 000, and more preferably ⁇ Is from 10 to 50,000.
- the average molecular weight of sericin is 300,000 or less, it is possible to prevent the aqueous solution from gelling at a concentration of about 0.5% by weight and to prevent the use form from being limited.
- the average molecular weight of sericin being 3,000 or more is advantageous for exerting a cell cryoprotective effect.
- the content of sericin in the composition for cell cryopreservation is not particularly limited, but sericin is contained so that the final concentration is 0.01 to 10.0% by weight. More preferably, sericin is contained so that the final concentration is 0.1 to 1% by weight.
- a final concentration of sericin of 0.01% by weight or more is advantageous for achieving a sufficient cryoprotective effect, and a concentration of 10.0% by weight or less is advantageous for suppressing an increase in storage cost. It is.
- the cell cryopreservation composition contains sericin so that the final concentration is 0.01 to L0% by weight. When used as a stock solution for use, this means that the concentration of sericin in the solution is 0.01-: L0.0% by weight. The same applies to amino acids and saccharides.
- amino acids include both optical isomers, that is, D-form and L-form.
- the amino acids mentioned here include not only the 20 ⁇ -amino acids that constitute natural proteins, but also other ⁇ -amino acids, as well as j8-, ⁇ -, ⁇ -amino acids and unnatural amino acids. Etc. may be included.
- the amino acids are alan, norine, leucine, isoleucine, proline, phenylalanine, tryptophan, methionine, glycine, serine, thread nin, cysteine, glutamine, asparagine, tyrosine, lysine, arginine.
- Aspartic acid, and glutamic acid power may be at least one selected from the group consisting of.
- the amino acid is one or more selected from the group consisting of proline, glutamine, serine, threonine, glycine, and alanine. More preferably, the amino acid is one or more selected from the group consisting of proline and glutamine.
- amino acid is used in the sense of including an amino acid derivative in addition to the amino acid itself.
- amino acid derivatives include amino acid salts and amino acid solvates.
- amino acid salt for example, alkali metal or alkaline earth metal salt such as sodium salt, potassium salt or calcium salt, hydrofluoric acid salt, hydrochloric acid salt, hydrobromic acid salt, hydroiodic acid Hydrohalic acid salts such as salts, inorganic acid salts such as nitrates, perchlorates, sulfates, phosphates, fumaric acid, succinates, succinates, oxalates, maleates, acetates And organic acid salts such as lactate and ascorbate.
- solvates of amino acids include hydrates, alcohol solvates (for example, methanol solvates, ethanol solvates), and ether solvates (for example, jet ether solvates).
- the content of amino acids in the composition for cell cryopreservation is not particularly limited, but the amino acids are contained so that the final concentration is 0.01 to LO: 0% by weight. More preferably, the amino acid is contained so that the final concentration is 0.1 to 1.0% by weight. A final amino acid concentration within the above range is advantageous for exhibiting a sufficient cryoprotective effect.
- the saccharide includes monosaccharides, oligosaccharides such as disaccharides, and polysaccharides.
- examples of the saccharide include glucose, xylose, arabinose, fructose, galactose, mannose, mannitol, sorbitol, xylitolole, myo-inositol, trenorose, sucrose, ratatose, manoletos, cereal biose, lactitonole, manoletonole, Noresenorelose, canoleboxymethinorescenose, dextran, glycogen, amylose, amylopectin, inulin, sodium alginate, ethinoresenorelose, hydroxyethinoresenorelose, raffinose, stachyose, xanthan gum, darcosamine And one or more selected from the group consisting of galactosamine.
- the saccharide is a maltose type disaccharide.
- maltose-type disaccharides include, for example, maltose, cellobiose, and ratatose.
- the saccharide is maltose.
- the saccharide content in the composition for cell cryopreservation is not particularly limited, but the saccharide should be contained so that the final concentration is 0.01 to LO: 0% by weight. More preferably, sugars are included so that the final concentration is 0.1 to 1.0% by weight.
- the final concentration of the saccharide within the above range is advantageous for exhibiting a sufficient cryoprotective effect.
- the composition for cell cryopreservation according to the present invention comprises at least sericin, an amino acid, and a saccharide.
- the compounding ratio (weight ratio) of these components in the composition for cell cryopreservation is typically ⁇ , as a weight ratio of sericin: amino acid: saccharide, from 1: 0.01 to LO: 0: 0. 01 ⁇ : LO. 0, more preferably ⁇ 1: 0. 1 ⁇ 1. 0: 0. 1 ⁇ 1.0, most preferably 1: 0. 4 ⁇ 0.8: 8: 0.3 ⁇ 0.7.
- composition for cell cryopreservation according to the present invention may further comprise other cell cryoprotective components.
- the other cell cryoprotective component is, for example, selected from the group consisting of dimethyl sulfoxide (DMSO), ethylene glycol, propylene glycol and glycerol. Two or more of these may be used in combination.
- the content of other cell cryoprotective components in the composition for cell cryopreservation according to the present invention can be appropriately changed depending on the type of components used, but preferably the final concentration is 1.0.
- the amount is -400.0% by weight, more preferably 5.0-20% by weight.
- the content of other cell cryoprotective components is 1.0% by weight or more, it is advantageous for obtaining a sufficient cryoprotective effect, and when it is 40.0% by weight or less, toxicity to cells is obtained.
- the content of dimethyl sulfoxide in the composition for cell cryopreservation is preferably 5.0 to 15% by weight.
- the other cell cryoprotective component is glycerol
- the glycerol content in the cell cryopreservation composition is preferably 5.0 to 20% by weight. From the viewpoint of cell cryoprotective effect, dimethyl sulfoxide is preferable to glycerol.
- the composition for cell cryopreservation according to the present invention may further comprise a buffer component.
- the buffer component is 6.0 to 8.5, preferably 7. when the composition for cell cryopreservation is a cryopreservation solution in a solution state and the ⁇ of the preservation solution is at room temperature (for example, 25 ° C). It is desirable to be selected to be 0 to 7.5. It is advantageous that the pH range is within the above-mentioned range because the influence on cell growth can be reduced.
- the buffer component may be any type as long as the above pH condition is satisfied.
- the buffer component include phosphates (for example, PBS), BES, TES, acetamidoglycine, glycinamide, glycylglycine, TRICINE, triethanolamine, veronal, and HEPES.
- the buffer component is selected from the group consisting of PBS, TRICINE, and HEPES, more preferably HEPES.
- the concentration of the buffer component contained therein is preferably 1 to: more preferably 1 to LOOOmM. 200 mM, more preferably 5 to 200 mM, even more preferably 5 to 50 mM.
- concentration of the buffer component is within the above range, a buffer effect can be obtained in the cryopreservation solution, and at the same time, the influence on the cells can be suppressed.
- composition for cell cryopreservation according to the present invention may further comprise other optional components.
- peptides e.g., peptides, other proteins, sugar alcohols, ⁇ amino sugars, glycoproteins, and additional components such as alcohol, P H adjusting agents, humectants, preservatives, viscosity adjusting agents, etc. Is mentioned. Two or more of these may be used in combination.
- the other optional components can be appropriately selected in terms of the type and amount used in consideration of the type of cells to be frozen, the freezing method, the freezing period, and the like.
- composition for cell cryopreservation preferably contains substantially no conventional culture solution or serum-derived components, but if necessary, denies the use of all or a part thereof. It ’s not something.
- the composition for cell cryopreservation comprises components (a) to (d) in the following amounts:
- an additional cell cryoprotective component selected from the group consisting of dimethyl sulfoxide, ethylene glycol, propylene glycol, and glycerol power
- the composition for cell cryopreservation comprises components (a) to (d) in the following amounts:
- an additional cell cryoprotective component selected from the group consisting of dimethyl sulfoxide, ethylene glycol, propylene glycol, and glycerol power
- composition for cell cryopreservation according to the present invention can be obtained by mixing the above-described components into a solid state as it is, or, if necessary, by dissolving in water to obtain an aqueous solution state.
- the composition for cell cryopreservation according to the present invention is used for cryopreservation of cells. That is, when carrying out the cryopreservation processing of the cells, the target cells are suspended in the composition according to the present invention in a solution state, and the cells are kept under the conditions for cryopreservation and frozen. When the cells are needed, they can be recovered after thawing the frozen cells and the composition according to the present invention.
- the target cell is contained in the composition for cell cryopreservation according to the present invention. And a method for cryopreserving the cells, comprising cryopreserving them.
- the cell concentration is preferably 10,000 to 10 million cells / ml! /.
- the method for freezing cells is not particularly limited.
- the suspension of the target cells in the cell cryopreservation composition in a solution state according to the present invention is placed in a freezing tube and directly placed in an ultra-low temperature freezer at 80 ° C.
- the cryotubes may be slowly frozen by placing them in a program freezer. Storage of frozen cells can be performed, for example, by maintaining the cells at the frozen temperature (for example, ⁇ 80 ° C.).
- the method for thawing the cells is not particularly limited, and for example, the frozen cells may be thawed by placing them in a 37 ° C. water bath to thaw the cells.
- the combination of sericin preferably comprises at least sericin, an amino acid, and a saccharide.
- this aqueous solution was concentrated using an evaporator until the sericin concentration was about 2%, and then lyophilized to obtain a sericin powder having a purity of 90% or more and an average molecular weight of about 30,000. It was.
- the average molecular weight of the obtained sericin was measured by gel filtration chromatography (LC 9A (manufactured by Shimadzu Corporation) as a measuring instrument) and Superdex 75HR 10/30 (manufactured by Pharmacia) as a column. ).
- Sericin powder was obtained in the same manner as described above except that the extraction solvent used for extracting sericin from the silk thread was distilled water. In this case, the average molecular weight of the obtained sericin was about 100,000.
- a sericin powder was obtained in the same manner as above except that the extraction solvent used for extracting sericin from the silk thread was 0.5% aqueous sodium hydrogen carbonate solution. In this case, the average molecular weight of the obtained sericin was about 10,000.
- Table 1 below shows the relationship between the type of extraction solvent and the average molecular weight of the obtained sericin.
- Example 1 Ingredient review a (sugar town)
- cryopreservation solution a composition for cell cryopreservation in a solution state (hereinafter sometimes referred to as “cryopreservation solution” or simply “preservation solution”) according to the composition shown in Table 2 below. It was.
- sericin having an average molecular weight of about 30,000 obtained above was used.
- PBS phosphate concentration 9.5 mM
- FBS fetal bovine serum
- the test uses RPMI 1640 medium (Asahi Techno Glass Co., Ltd.) containing 10.0% FBS.
- Cells 0 were suspended in each cryopreservation solution so that the cell concentration was about 1.0 ⁇ 10 6 cells / ml, and each 1 ml was added to a freezing tube (Asahi Techno Glass Co., Ltd.). The cryotubes are then stored frozen for 30 days using a -80 ° C freezer and then placed in a 37 ° C water bath.
- the preservation solution a4 supplemented with maltose showed an excellent cryoprotective effect compared to the preservation solution containing only sericin (preservation solution a2) and other saccharides.
- Example 2 Review of ingredients b (amino acids)
- Cryopreservation solutions bl to b9 were prepared in the same manner as in Example 1 except that the composition shown in Table 4 was followed.
- the cryoprotective effect of preservation solution b 7 was comparable to that using serum (preservation solution bl).
- Example 3 Ingredients c (other fine frozen components)
- Cryopreservation solutions cl to c6 were prepared in the same manner as in Example 1 except that the composition shown in Table 6 below was followed.
- Cryopreservation test is also performed as a cryopreservation solution.
- Stock solutions (stock solutions c2 to c5) to which dimethyl sulfoxide, ethylene glycol, propylene glycol and glycerol were added showed excellent cryoprotective effects.
- Example 4 5Fe> of ⁇ d (buffer components)
- the cryopreservation test was performed in the same manner as in Example 1 except that the preservation solution dl d6 was used as the cryopreservation solution.
- the stock solution (d2 d6) supplemented with PBS HEPES and TRICINE showed excellent cryoprotective effect.
- the cryoprotective effect was excellent at any measured buffer component concentration (d4 d6).
- Example 5 Review of ingredients e (average molecular weight of sericin)
- sedicine having an average molecular weight of about 10,000, about 30,000, and about 100,000, respectively, obtained in the section “Preparation of Sericin” was used.
- the cryopreservation test was performed in the same manner as in Example 1 except that the preservation solution ele6 was used as the cryopreservation solution.
- cryopreservation solution e3 The cryopreservation solution to which sericin having an average molecular weight of 30,000 was added (preservation solution e3) showed an excellent cryoprotective effect as compared with the case of adding lower molecular weight sericin and higher molecular weight sericin.
- Example 6 The performance of cryopreservation of other fine baskets
- Example 5 The test was performed in the same manner as in the cryopreservation test of Example 1 except that the cells used were changed to other cell types and the cryopreservation solutions were the cryopreservation solutions el, e3 and e6 in Example 5.
- NHDF normal human dermal fibroblasts
- PC-12 rat adrenal medulla brown cell line
- Sf—9 obtained from Human Science Foundation
- CHO Choinese Hamster ovary
- RIKEN human epidermal keratinocytes
- HEK human epidermal keratinocytes
- RC4 primary corneal corneal cells
- NHDF is used for wound therapy and cytotoxicity studies.
- Hypridoma cells produce monoclonal antibodies, and CHO cells and Sf-9 cells are transformed by genetic engineering techniques to produce physiologically active substances. These cells are used industrially as protein production means.
- HepG2 cells are useful as models for artificial livers, and PC-12 cells are useful as materials for investigating the process of separation into neurons.
- HEK cells are used for skin irritation tests, and RC4 cells are used for eye toxicity tests for cosmetics and pesticides.
- the normal freezing and thawing operation was repeated, followed by repeated freezing and freezing operations, and then the survival rate was measured.
- cryopreservation solution (preservation solution e3) showed the same survival rate as when serum was used (preservation solution el) in cells of different origins.
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Abstract
Description
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Priority Applications (2)
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US11/663,539 US9055739B2 (en) | 2004-09-24 | 2005-09-26 | Compositions for cryopreservation of cells |
EP05785739.3A EP1820847B1 (en) | 2004-09-24 | 2005-09-26 | Composition for cell frozen-storage |
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JP2004276951 | 2004-09-24 | ||
JP2004-276951 | 2004-09-24 |
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WO2006033429A1 true WO2006033429A1 (ja) | 2006-03-30 |
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EP (1) | EP1820847B1 (ja) |
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Cited By (2)
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CN101310009B (zh) * | 2005-11-17 | 2016-01-06 | 日本全药工业株式会社 | 细胞保存用水溶液 |
US20130236960A1 (en) * | 2010-11-19 | 2013-09-12 | Yoshihiro Kunitomi | Vitrificated storage solution for cells |
CN103103124A (zh) * | 2013-02-18 | 2013-05-15 | 上海交通大学 | 丝胶肽在冷冻干燥或冷冻保藏益生菌中的应用 |
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US10314302B2 (en) * | 2015-12-16 | 2019-06-11 | Regents Of The University Of Minnesota | Cryopreservative compositions and methods |
TWI670371B (zh) | 2016-10-04 | 2019-09-01 | 全崴生技股份有限公司 | 用於細胞冷凍保存的組成物和方法 |
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Also Published As
Publication number | Publication date |
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EP1820847B1 (en) | 2014-06-11 |
US9055739B2 (en) | 2015-06-16 |
EP1820847A1 (en) | 2007-08-22 |
EP1820847A4 (en) | 2007-10-03 |
US20090197331A1 (en) | 2009-08-06 |
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