WO2006031840A3 - Increasing the stability of recombinant adult human apohemoglobin - Google Patents

Increasing the stability of recombinant adult human apohemoglobin Download PDF

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Publication number
WO2006031840A3
WO2006031840A3 PCT/US2005/032627 US2005032627W WO2006031840A3 WO 2006031840 A3 WO2006031840 A3 WO 2006031840A3 US 2005032627 W US2005032627 W US 2005032627W WO 2006031840 A3 WO2006031840 A3 WO 2006031840A3
Authority
WO
WIPO (PCT)
Prior art keywords
rhb
apo
adult human
apohemoglobin
stability
Prior art date
Application number
PCT/US2005/032627
Other languages
French (fr)
Other versions
WO2006031840A2 (en
Inventor
John S Olson
George N Phillips Jr
Original Assignee
Univ Rice William M
Wisconsin Alumni Res Found
John S Olson
George N Phillips Jr
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Rice William M, Wisconsin Alumni Res Found, John S Olson, George N Phillips Jr filed Critical Univ Rice William M
Priority to EP05796733A priority Critical patent/EP1799839A2/en
Publication of WO2006031840A2 publication Critical patent/WO2006031840A2/en
Publication of WO2006031840A3 publication Critical patent/WO2006031840A3/en
Priority to US11/724,007 priority patent/US7803912B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/795—Porphyrin- or corrin-ring-containing peptides
    • C07K14/805—Haemoglobins; Myoglobins

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The disclosure relates to recombinant adult human apohemoglobin (apo-rHb) in which the stability has been increased by replacement of an amino acid with a counterpart from another organsim, such as a deep sea diving mammal. This mutated apo-rHb may be more stable and/or give higher production yields than unmutated adult human apo-rHb. The mutated apo-rHb may be produced in microorganisms, such as E. coli or yeast cells, or animal erythroid cells. Some apo-rHb of the present disclosure may be used as part of a blood substitute.
PCT/US2005/032627 2004-09-15 2005-09-15 Increasing the stability of recombinant adult human apohemoglobin WO2006031840A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP05796733A EP1799839A2 (en) 2004-09-15 2005-09-15 Increasing the stability of recombinant adult human apohemoglobin
US11/724,007 US7803912B2 (en) 2004-09-15 2007-03-14 Increasing the stability of recombinant adult human apohemoglobin

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US61010904P 2004-09-15 2004-09-15
US61011004P 2004-09-15 2004-09-15
US61010804P 2004-09-15 2004-09-15
US60/610,108 2004-09-15
US60/610,110 2004-09-15
US60/610,109 2004-09-15

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US11/724,007 Continuation US7803912B2 (en) 2004-09-15 2007-03-14 Increasing the stability of recombinant adult human apohemoglobin

Publications (2)

Publication Number Publication Date
WO2006031840A2 WO2006031840A2 (en) 2006-03-23
WO2006031840A3 true WO2006031840A3 (en) 2006-05-11

Family

ID=36060656

Family Applications (3)

Application Number Title Priority Date Filing Date
PCT/US2005/032627 WO2006031840A2 (en) 2004-09-15 2005-09-15 Increasing the stability of recombinant adult human apohemoglobin
PCT/US2005/033027 WO2006031992A2 (en) 2004-09-15 2005-09-15 Increasing hemoglobin and other heme protein production in bacteria by co-expression of heme transport genes
PCT/US2005/033028 WO2006031993A2 (en) 2004-09-15 2005-09-15 Enhancing recombinant hemoglobin production by co-expression with alpha hemoglobin stabilizing protein

Family Applications After (2)

Application Number Title Priority Date Filing Date
PCT/US2005/033027 WO2006031992A2 (en) 2004-09-15 2005-09-15 Increasing hemoglobin and other heme protein production in bacteria by co-expression of heme transport genes
PCT/US2005/033028 WO2006031993A2 (en) 2004-09-15 2005-09-15 Enhancing recombinant hemoglobin production by co-expression with alpha hemoglobin stabilizing protein

Country Status (3)

Country Link
US (1) US7803912B2 (en)
EP (3) EP1799839A2 (en)
WO (3) WO2006031840A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014124402A2 (en) 2013-02-11 2014-08-14 Becton, Dickinson And Company Chromophore compositions and methods of making and using the same
WO2015100235A1 (en) 2013-12-27 2015-07-02 Virginia Commonwealth University Allosteric hemoglobin modifiers with nitric oxide releasing moiety
SE2051317A1 (en) * 2020-11-11 2022-05-12 Nielsen Dina Petranovic A genetically modified yeast cell

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6022849A (en) * 1987-05-16 2000-02-08 Baxter Biotech Technology Saarl Mutant recombinant hemoglobins containing heme pocket mutations
GB8711614D0 (en) * 1987-05-16 1987-06-24 Medical Res Council Proteins
US6204009B1 (en) * 1988-05-16 2001-03-20 BAXTER BIOTECH TECHNOLOGY SàRL Nucleic acids encoding mutant recombinant hemoglobins containing heme pocket mutations
US6172039B1 (en) * 1990-04-16 2001-01-09 Apex Bioscience, Inc. Expression of recombinant hemoglobin and hemoglobin variants in yeast
US5239061A (en) * 1990-06-20 1993-08-24 Research Corporation Technologies, Inc. Modified human hemoglobin, blood substitutes containing the same, and vectors for expressing the modified hemoglobin
JP3399549B2 (en) * 1990-11-16 2003-04-21 サントリー株式会社 Microbial peroxidase gene
US5240831A (en) * 1991-01-10 1993-08-31 Board Of Regents, The University Of Texas Methods and compositions for the expression of biologically active eukaryotic cytochrome p45os in bacteria
US5744340A (en) 1995-06-09 1998-04-28 Schering Corporation Expression of human inducible nitric oxide synthase
WO1997015591A1 (en) * 1995-10-23 1997-05-01 Rice University Hemoglobin mutants that reduce heme loss
EP0979279B1 (en) * 1997-05-02 2008-03-19 Baxter Biotech Technology S.A.R.L. Hemoglobin mutants with reduced nitric oxide scavenging
EP1294868A2 (en) * 2000-06-29 2003-03-26 Incyte Genomics, Inc. Adenylyl and guanylyl cyclases
TWI253155B (en) * 2003-05-28 2006-04-11 Siliconware Precision Industries Co Ltd Thermally enhanced semiconductor package and fabrication method thereof
US20070166792A1 (en) * 2004-09-15 2007-07-19 Olson John S Increasing hemoglobin and other heme protein production in bacteria by co-expression of heme transport genes
US7642233B2 (en) * 2004-09-15 2010-01-05 William Marsh Rice University Enhancing recombinant hemoglobin production by co-expression with alpha hemoglobin stabilizing protein

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DUMOULIN A. ET AL: "The N-terminal sequence affects distant helix interactions in hemoglobin", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 273, no. 52, 25 December 1998 (1998-12-25), pages 35032 - 35038, XP002996148 *
FRONTICELLI C. ET AL: "Allosteric modulation by tertiary structure in mammalian hemoglobins", JOURNAL OF BOIOLOGICAL CHEMISTRY, vol. 270, no. 51, 22 December 1995 (1995-12-22), pages 30588 - 30592, XP002996147 *

Also Published As

Publication number Publication date
EP1799234A2 (en) 2007-06-27
EP1797193A2 (en) 2007-06-20
EP1797193A4 (en) 2009-08-26
WO2006031992A2 (en) 2006-03-23
EP1799839A2 (en) 2007-06-27
EP1799234A4 (en) 2009-02-18
WO2006031993A3 (en) 2007-01-11
WO2006031993A2 (en) 2006-03-23
US20070172924A1 (en) 2007-07-26
WO2006031992A3 (en) 2007-02-15
WO2006031840A2 (en) 2006-03-23
US7803912B2 (en) 2010-09-28
WO2006031992A9 (en) 2006-06-15

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