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Particle/cell separation device and compositions

Info

Publication number
WO2006023502A3
WO2006023502A3 PCT/US2005/029136 US2005029136W WO2006023502A3 WO 2006023502 A3 WO2006023502 A3 WO 2006023502A3 US 2005029136 W US2005029136 W US 2005029136W WO 2006023502 A3 WO2006023502 A3 WO 2006023502A3
Authority
WO
Grant status
Application
Patent type
Prior art keywords
device
rich
cell
separation
particle
Prior art date
Application number
PCT/US2005/029136
Other languages
French (fr)
Other versions
WO2006023502A2 (en )
Inventor
Allan Mishra
Original Assignee
Allan Mishra
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date

Links

Classifications

    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K35/00Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/14Blood; Artificial blood
    • A61K35/16Blood plasma; Blood serum
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D33/00Filters with filtering elements which move during the filtering operation
    • B01D33/0003Aspects of filters with filtering elements which move during the filtering operation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0081Purging biological preparations of unwanted cells
    • C12N5/0087Purging against subsets of blood cells, e.g. purging alloreactive T cells

Abstract

A particle / cell separation device is described which is particularly adapted for neutrophil depletion from a preparation of whole blood or platelet-rich plasma. Also described are blood and platelet rich plasma compositions produced using the device which are neutrophil-depleted.
PCT/US2005/029136 2004-08-20 2005-08-17 Particle/cell separation device and compositions WO2006023502A3 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US60324904 true 2004-08-20 2004-08-20
US60/603,249 2004-08-20

Publications (2)

Publication Number Publication Date
WO2006023502A2 true WO2006023502A2 (en) 2006-03-02
WO2006023502A3 true true WO2006023502A3 (en) 2006-06-01

Family

ID=35968112

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/029136 WO2006023502A3 (en) 2004-08-20 2005-08-17 Particle/cell separation device and compositions

Country Status (2)

Country Link
US (4) US7462268B2 (en)
WO (1) WO2006023502A3 (en)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7608258B2 (en) * 2002-04-13 2009-10-27 Allan Mishra Method for treatment of tendinosis using platelet rich plasma
US6811777B2 (en) * 2002-04-13 2004-11-02 Allan Mishra Compositions and minimally invasive methods for treating incomplete connective tissue repair
WO2005065269A3 (en) * 2003-12-29 2005-12-15 Allan Mishra Compositions and method for decreasing the appearance of skin wrinkles
WO2005065242A3 (en) * 2003-12-29 2006-06-22 Am Biosolutions Method of treating cancer using platelet releasate
WO2005065419A3 (en) * 2003-12-29 2005-10-06 Am Biosolutions Method of culturing cells
US7462268B2 (en) 2004-08-20 2008-12-09 Allan Mishra Particle/cell separation device and compositions
US20060095121A1 (en) * 2004-10-28 2006-05-04 Medtronic Vascular, Inc. Autologous platelet gel on a stent graft
US20070172472A1 (en) * 2005-06-23 2007-07-26 Asha Nayak Methods and Systems for Treating Injured Cardiac Tissue
US20070093748A1 (en) * 2005-06-23 2007-04-26 Medtronic Vascular, Inc. Methods and systems for treating injured cardiac tissue
US20070042016A1 (en) * 2005-06-23 2007-02-22 Medtronic Vascular, Inc. Methods and Systems for Treating Injured Cardiac Tissue
WO2010042658A1 (en) * 2008-10-07 2010-04-15 Bioparadox, Llc Use of platelet rich plasma composition in the treatment of cardiac conduction abnormalities
WO2010042762A1 (en) 2008-10-09 2010-04-15 Bioparadox, Llc Platelet rich plasma formulations for cardiac treatments
US20100233282A1 (en) * 2009-03-13 2010-09-16 Allan Mishra Device and methods for delivery of bioactive materials to the right side of the heart
US20120171181A1 (en) 2009-09-04 2012-07-05 Allan Mishra Compositions and minimally invasive methods for treating cancer
WO2011127071A1 (en) * 2010-04-05 2011-10-13 Allan Mishra Platelet rich plasma formulations

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4931395A (en) * 1986-12-08 1990-06-05 Dana-Farber Cancer Institute Monoclonal antibody specific to neutrophils
US5403272A (en) * 1992-05-29 1995-04-04 Baxter International Inc. Apparatus and methods for generating leukocyte free platelet concentrate
US5585007A (en) * 1994-12-07 1996-12-17 Plasmaseal Corporation Plasma concentrate and tissue sealant methods and apparatuses for making concentrated plasma and/or tissue sealant
US20020058030A1 (en) * 2000-05-03 2002-05-16 Eligix, Inc. Whole blood separator apparatus and method of use
US20030116512A1 (en) * 2001-12-05 2003-06-26 Glen Delbert Antwiler Methods and apparatus for separation of particles
US6643786B1 (en) * 1999-01-25 2003-11-04 Kabushiki Kaisha Toshiba Computer battery event managing method with option to compulsorily execute a low battery capacity operation even when a program of the computer does not respond
US6811777B2 (en) * 2002-04-13 2004-11-02 Allan Mishra Compositions and minimally invasive methods for treating incomplete connective tissue repair

Family Cites Families (89)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4081331A (en) * 1976-04-05 1978-03-28 Gunther Weiss Portable water distillation apparatus
US4414108A (en) * 1980-05-09 1983-11-08 The United States Of America As Represented By The Department Of Health And Human Services Apparatus and method for continuous countercurrent extraction and particle separation
CA1244774A (en) 1983-11-09 1988-11-15 Thomas Jefferson University Medium for storing blood platelets
US5428008A (en) * 1989-04-14 1995-06-27 Prp, Inc. Therapeutic composition of micellar structures capable of promoting hemotasis
DE3586844T2 (en) * 1984-06-22 1994-03-17 Richard L Veech Electrolyte solutions and their use (in vivo).
US5165938A (en) * 1984-11-29 1992-11-24 Regents Of The University Of Minnesota Wound healing agents derived from platelets
US5178883A (en) * 1984-11-29 1993-01-12 Regents Of The University Of Minnesota Method for promoting hair growth
EP0267286B1 (en) * 1986-03-28 1993-05-26 ASAHI MEDICAL Co., Ltd. Filter medium for selectively removing leucocytes
US5124316A (en) * 1986-11-14 1992-06-23 President And Fellows Of Harvard College Method for periodontal regeneration
US5079236A (en) * 1987-05-27 1992-01-07 Hyal Pharmaceutical Corporation Pure, sterile, pyrogen-free hyaluronic acid formulations their methods of preparation and methods of use
US5344561A (en) * 1989-05-09 1994-09-06 Pall Corporation Device for depletion of the leucocyte content of blood and blood components
WO1991004035A1 (en) 1989-09-15 1991-04-04 Curative Technologies, Inc. Selecting amounts of platelet releasate for efficacious treatment of tissue
US5599558A (en) * 1989-09-15 1997-02-04 Curative Technologies, Inc. Selecting amounts of platelet releasate for efficacious treatment of tissue
US5147776A (en) * 1990-02-26 1992-09-15 University Of Iowa Research Foundation Use of 2,5-anhydromannitol for control of pH during blood storage
DK0547064T3 (en) * 1990-07-23 1995-03-20 Zymogenetics Inc Protease Resistant PDGF and application procedure
US5336387A (en) * 1990-09-11 1994-08-09 Bioseparations, Inc. Electrical separator apparatus and method of counterflow gradient focusing
US5641622A (en) * 1990-09-13 1997-06-24 Baxter International Inc. Continuous centrifugation process for the separation of biological components from heterogeneous cell populations
US5385937A (en) * 1991-04-10 1995-01-31 Brigham & Women's Hospital Nitrosation of homocysteine as a method for treating homocysteinemia
US5474891A (en) * 1991-10-30 1995-12-12 Thomas Jefferson University Plasma-based platelet concentrate preparations with additive
US5785869A (en) * 1992-02-10 1998-07-28 Baxter International Inc. Method for creating a leukocyte rich sample from a mixed population of blood cells
US5269290B1 (en) * 1992-04-17 1996-06-11 Instratek Inc Plantar fasciotomy surgical procedure
EP1338648B1 (en) * 1992-05-29 2005-11-30 East Carolina University Pharmaceutically acceptable fixed-dried human blood platelets
US5324284A (en) * 1992-06-05 1994-06-28 Cardiac Pathways, Inc. Endocardial mapping and ablation system utilizing a separately controlled ablation catheter and method
US5494590A (en) * 1992-06-11 1996-02-27 Becton Dickinson Method of using anticoagulant solution in blood separation
US5449688A (en) * 1993-03-30 1995-09-12 The United States Of America As Represented By The Department Of Health And Human Services Method of treating chronic inflammatory diseases
EP0720658A1 (en) * 1993-09-23 1996-07-10 E.I. Du Pont De Nemours And Company An electrophoretic method for the isolation and separation of microorganisms
US5662813A (en) * 1994-10-21 1997-09-02 Bioseparations, Inc. Method for separation of nucleated fetal erythrocytes from maternal blood samples
US5789147A (en) * 1994-12-05 1998-08-04 New York Blood Center, Inc. Method for concentrating white cells from whole blood by adding a red cell sedimentation reagent to whole anticoagulated blood
US7169547B2 (en) * 1994-12-05 2007-01-30 New York Blood Center, Inc. High concentration white blood cells as a therapeutic product
US5510102A (en) * 1995-01-23 1996-04-23 The Regents Of The University Of California Plasma and polymer containing surgical hemostatic adhesives
US5614204A (en) * 1995-01-23 1997-03-25 The Regents Of The University Of California Angiographic vascular occlusion agents and a method for hemostatic occlusion
US5643786A (en) * 1995-01-27 1997-07-01 The United States Of America As Represented By The Department Of Health And Human Services Method for isolating dendritic cells
WO1996028476A1 (en) * 1995-03-13 1996-09-19 Novogen Research Ltd. Process for glucan preparation and therapeutic uses of glucan
US5913768A (en) * 1995-04-18 1999-06-22 Cobe Laboratories, Inc. Particle filter apparatus
US5814022A (en) * 1996-02-06 1998-09-29 Plasmaseal Llc Method and apparatus for applying tissue sealant
US5834418A (en) * 1996-03-20 1998-11-10 Theratechnologies, Inc. Process for the preparation of platelet growth factors extract
JP2000509307A (en) * 1996-04-30 2000-07-25 メドトロニック,インコーポレイテッド Manufacturing method of autologous fibrin sealant
WO2000062828A1 (en) * 1996-04-30 2000-10-26 Medtronic, Inc. Autologous fibrin sealant and method for making the same
US6712851B1 (en) * 1998-01-23 2004-03-30 Macropore Biosurgery, Inc. Resorbable, macro-porous non-collapsing and flexible membrane barrier for skeletal repair and regeneration
US6280473B1 (en) * 1996-08-19 2001-08-28 Macropore, Inc. Resorbable, macro-porous, non-collapsing and flexible membrane barrier for skeletal repair and regeneration
US5935850A (en) * 1996-09-30 1999-08-10 The Research Foundation Of State University Of New York Model for cell migration and use thereof
US6432119B1 (en) * 1999-03-17 2002-08-13 Angiotrax, Inc. Apparatus and methods for performing percutaneous myocardial revascularization and stimulating angiogenesis using autologous materials
US6120520A (en) * 1997-05-27 2000-09-19 Angiotrax, Inc. Apparatus and methods for stimulating revascularization and/or tissue growth
DE69931170D1 (en) 1998-08-26 2006-06-08 Neomend Inc Kit for the in situ generation of chemically combined mechanical barrier or cap structures for a puncture site in a blood vessel
JP5264026B2 (en) * 1997-06-10 2013-08-14 エルパス・インコーポレイテッドLpath, Inc. A method for the early detection of heart disease
US6098631A (en) * 1998-01-21 2000-08-08 The Regents Of The University Of Michigan Compositions and methods for treating autoimmune disease
US6183442B1 (en) * 1998-03-02 2001-02-06 Board Of Regents Of The University Of Texas System Tissue penetrating device and methods for using same
DE69903732D1 (en) 1998-07-02 2002-12-05 Stichting Skeletal Tissue Engi Bone cement with antimicrobial peptides
US6322785B1 (en) * 1999-03-02 2001-11-27 Natrex Technologies Methods and compositions for bone graft implants
US6315992B1 (en) * 1999-06-30 2001-11-13 Tissuegene Co. Generating cartilage in a mammal using fibroblasts transfected with a vector encoding TGF-β-1
US6454804B1 (en) * 1999-10-08 2002-09-24 Bret A. Ferree Engineered tissue annulus fibrosis augmentation methods and apparatus
DE19960504A1 (en) * 1999-12-15 2001-08-16 Curasan Ag regeneration means
US6592566B2 (en) * 2000-02-03 2003-07-15 Arizona Board Of Regents Method for forming an endovascular occlusion
US20020006437A1 (en) * 2000-05-01 2002-01-17 Grooms Jamie M. Non-migration tissue capsule
KR100880622B1 (en) * 2000-06-29 2003-04-07 바이오신텍 캐나다 인코포레이티드 Composition and method for the repair and regeneration of cartilage and other tissues
US20030185812A1 (en) * 2000-06-30 2003-10-02 Ferree Bret A. Method of treating dural leaks with platelet-rich plasma (PRP)
US20020032155A1 (en) * 2000-06-30 2002-03-14 Ferree Bret A. Method of treating disc herniation and disc degeneration with concentrated growth and differentiation factors
US6554801B1 (en) * 2000-10-26 2003-04-29 Advanced Cardiovascular Systems, Inc. Directional needle injection drug delivery device and method of use
US7628780B2 (en) * 2001-01-13 2009-12-08 Medtronic, Inc. Devices and methods for interstitial injection of biologic agents into tissue
US6447443B1 (en) * 2001-01-13 2002-09-10 Medtronic, Inc. Method for organ positioning and stabilization
US7740623B2 (en) * 2001-01-13 2010-06-22 Medtronic, Inc. Devices and methods for interstitial injection of biologic agents into tissue
US6942880B1 (en) * 2001-04-09 2005-09-13 Medtronic, Inc. Autologous platelet gel having beneficial geometric shapes and methods of making the same
US20030152639A1 (en) * 2001-07-03 2003-08-14 Calvin Britton Novel wound healing composition not containing bovine-derived activating reagents
JP2005500881A (en) 2001-08-09 2005-01-13 アイボクラー ヴィヴェイデント インコーポレイテッド Tissue implant, as well as methods of making and using thereof
US7241281B2 (en) * 2002-04-08 2007-07-10 Thermogenesis Corporation Blood component separation method and apparatus
US20030192554A1 (en) * 2002-04-11 2003-10-16 Ferree Bret A. Methods and apparatus for adhering musculoskeletal tissues
US7608258B2 (en) * 2002-04-13 2009-10-27 Allan Mishra Method for treatment of tendinosis using platelet rich plasma
US20030205538A1 (en) * 2002-05-03 2003-11-06 Randel Dorian Methods and apparatus for isolating platelets from blood
US20030224455A1 (en) * 2002-05-28 2003-12-04 Mariapia Abbracchio Method for the diagnosis of heart diseases
US7166133B2 (en) * 2002-06-13 2007-01-23 Kensey Nash Corporation Devices and methods for treating defects in the tissue of a living being
US6982038B2 (en) * 2002-06-14 2006-01-03 Medtronic, Inc. Centrifuge system utilizing disposable components and automated processing of blood to collect platelet rich plasma
US7674250B2 (en) * 2002-08-05 2010-03-09 Boston Scientific Scimed, Inc. Methods of delivering therapeutic agents
DE60319848T2 (en) 2002-09-07 2009-04-30 Medcell Bioscience Ltd. Medicine with mesenchymal stem cells
US20040126885A1 (en) * 2002-11-05 2004-07-01 Cines Douglas B. Delivery vehicle for recombinant proteins
US6837848B2 (en) * 2003-01-15 2005-01-04 Medtronic, Inc. Methods and apparatus for accessing and stabilizing an area of the heart
US6905612B2 (en) * 2003-03-21 2005-06-14 Hanuman Llc Plasma concentrate apparatus and method
US20040182795A1 (en) * 2003-03-21 2004-09-23 Randel Dorian Apparatus and method for concentration of plasma from whole blood
US20050064042A1 (en) * 2003-04-29 2005-03-24 Musculoskeletal Transplant Foundation Cartilage implant plug with fibrin glue and method for implantation
US20040244806A1 (en) * 2003-06-09 2004-12-09 Ferree Bret A. Treating disc herniation and other conditions with leukocytes
US7338494B2 (en) * 2003-08-19 2008-03-04 Synthes (U.S.A.) Spring-loaded awl
WO2005065269A3 (en) * 2003-12-29 2005-12-15 Allan Mishra Compositions and method for decreasing the appearance of skin wrinkles
WO2005065242A3 (en) 2003-12-29 2006-06-22 Am Biosolutions Method of treating cancer using platelet releasate
WO2005065419A3 (en) * 2003-12-29 2005-10-06 Am Biosolutions Method of culturing cells
US7462268B2 (en) 2004-08-20 2008-12-09 Allan Mishra Particle/cell separation device and compositions
US7211191B2 (en) * 2004-09-30 2007-05-01 Thermogenesis Corp. Blood component separation method and apparatus
EP1848474B1 (en) * 2005-02-07 2013-06-12 Hanuman LLC Platelet rich plasma concentrate apparatus and method
US8092475B2 (en) * 2005-04-15 2012-01-10 Integra Lifesciences (Ireland) Ltd. Ultrasonic horn for removal of hard tissue
US20070042016A1 (en) * 2005-06-23 2007-02-22 Medtronic Vascular, Inc. Methods and Systems for Treating Injured Cardiac Tissue
WO2010042762A1 (en) * 2008-10-09 2010-04-15 Bioparadox, Llc Platelet rich plasma formulations for cardiac treatments

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4931395A (en) * 1986-12-08 1990-06-05 Dana-Farber Cancer Institute Monoclonal antibody specific to neutrophils
US5403272A (en) * 1992-05-29 1995-04-04 Baxter International Inc. Apparatus and methods for generating leukocyte free platelet concentrate
US5585007A (en) * 1994-12-07 1996-12-17 Plasmaseal Corporation Plasma concentrate and tissue sealant methods and apparatuses for making concentrated plasma and/or tissue sealant
US6643786B1 (en) * 1999-01-25 2003-11-04 Kabushiki Kaisha Toshiba Computer battery event managing method with option to compulsorily execute a low battery capacity operation even when a program of the computer does not respond
US20020058030A1 (en) * 2000-05-03 2002-05-16 Eligix, Inc. Whole blood separator apparatus and method of use
US20030116512A1 (en) * 2001-12-05 2003-06-26 Glen Delbert Antwiler Methods and apparatus for separation of particles
US6811777B2 (en) * 2002-04-13 2004-11-02 Allan Mishra Compositions and minimally invasive methods for treating incomplete connective tissue repair

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
YANG J ET AL: "Dielectric properties of human leukocyte subpopulations determined by electrorotation as a cell separation criterion.", BIOPHYSICAL JOURNAL., vol. 76, 1999, pages 3307 - 3314, XP002996512 *

Also Published As

Publication number Publication date Type
US7462268B2 (en) 2008-12-09 grant
US20060127382A1 (en) 2006-06-15 application
US20090092679A1 (en) 2009-04-09 application
WO2006023502A2 (en) 2006-03-02 application
US8142993B1 (en) 2012-03-27 grant
US20120085651A1 (en) 2012-04-12 application
US20160106779A1 (en) 2016-04-21 application

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