WO2006019954A3 - In vivo assays for modulation of smad-mediated signaling - Google Patents

In vivo assays for modulation of smad-mediated signaling Download PDF

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Publication number
WO2006019954A3
WO2006019954A3 PCT/US2005/025056 US2005025056W WO2006019954A3 WO 2006019954 A3 WO2006019954 A3 WO 2006019954A3 US 2005025056 W US2005025056 W US 2005025056W WO 2006019954 A3 WO2006019954 A3 WO 2006019954A3
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WO
WIPO (PCT)
Prior art keywords
smad
tgf
modulation
vivo assays
mediated signaling
Prior art date
Application number
PCT/US2005/025056
Other languages
French (fr)
Other versions
WO2006019954A2 (en
Inventor
Hung-Kam Cheung
Leona E Ling
Michael Parr
James G Barsoum
Original Assignee
Biogen Idec Inc
Hung-Kam Cheung
Leona E Ling
Michael Parr
James G Barsoum
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biogen Idec Inc, Hung-Kam Cheung, Leona E Ling, Michael Parr, James G Barsoum filed Critical Biogen Idec Inc
Priority to EP05791000A priority Critical patent/EP1781823A2/en
Publication of WO2006019954A2 publication Critical patent/WO2006019954A2/en
Publication of WO2006019954A3 publication Critical patent/WO2006019954A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The disclosure provides an assay system for evaluating compounds that modulate Smad3-mediated gene regulation, and the TGF-β/activin signaling pathway, in particular. The methods utilize a reporter construct responsive in vivo to a member of the TGF-β superfamily (e.g., TGF-β or activin). The reporter construct includes a tandem of N Smad-binding elements operably linked to a reporter gene, wherein N is greater than 12, for example, 25.
PCT/US2005/025056 2004-07-16 2005-07-14 In vivo assays for modulation of smad-mediated signaling WO2006019954A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP05791000A EP1781823A2 (en) 2004-07-16 2005-07-14 In vivo assays for modulation of smad-mediated signaling

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US58834304P 2004-07-16 2004-07-16
US60/588,343 2004-07-16

Publications (2)

Publication Number Publication Date
WO2006019954A2 WO2006019954A2 (en) 2006-02-23
WO2006019954A3 true WO2006019954A3 (en) 2006-05-18

Family

ID=35730591

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/025056 WO2006019954A2 (en) 2004-07-16 2005-07-14 In vivo assays for modulation of smad-mediated signaling

Country Status (2)

Country Link
EP (1) EP1781823A2 (en)
WO (1) WO2006019954A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2392862B1 (en) * 2011-06-02 2013-11-05 Fundación Para La Investigación Biomédica Del Hospital Universitario Ramón Y Cajal TGF-BETA AND / OR SMAD3 AS MARKERS OF DIAGNOSIS AND / OR FORECAST OF ALPHA-SINUCLEINOPATHIES
CN117677692A (en) * 2021-05-21 2024-03-08 江苏九济华生医药科技研究院有限公司 Cell strain for TGF beta detection and high-precision TGF beta detection method
CN114236137A (en) * 2021-12-17 2022-03-25 中山大学·深圳 In-vitro detection kit for transforming growth factor beta and application thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999045113A1 (en) * 1998-03-03 1999-09-10 Ludwig Institute For Cancer Research Smad binding elements and uses thereof
WO1999046384A2 (en) * 1998-03-13 1999-09-16 The Johns Hopkins University Human smad3 and smad4 are sequence-specific transcription activators

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999045113A1 (en) * 1998-03-03 1999-09-10 Ludwig Institute For Cancer Research Smad binding elements and uses thereof
WO1999046384A2 (en) * 1998-03-13 1999-09-16 The Johns Hopkins University Human smad3 and smad4 are sequence-specific transcription activators

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
THIES R S ET AL: "GDF-8 PROPEPTIDE BINDS TO GDF-8 AND ANTAGONIZES BIOLOGICAL ACTIVITY BY INHIBITING GDF-8 RECEPTOR BINDING", GROWTH FACTORS, HARWOOD ACADEMIC PUBLISHERS GMBH, vol. 18, no. 4, 2001, pages 251 - 259, XP008013942, ISSN: 0897-7194 *

Also Published As

Publication number Publication date
EP1781823A2 (en) 2007-05-09
WO2006019954A2 (en) 2006-02-23

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