WO2006018311A3 - Determination of nucleic acid analytes by treatment with microwaves - Google Patents

Determination of nucleic acid analytes by treatment with microwaves Download PDF

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Publication number
WO2006018311A3
WO2006018311A3 PCT/EP2005/008992 EP2005008992W WO2006018311A3 WO 2006018311 A3 WO2006018311 A3 WO 2006018311A3 EP 2005008992 W EP2005008992 W EP 2005008992W WO 2006018311 A3 WO2006018311 A3 WO 2006018311A3
Authority
WO
WIPO (PCT)
Prior art keywords
microwaves
nucleic acid
determination
treatment
acid analytes
Prior art date
Application number
PCT/EP2005/008992
Other languages
French (fr)
Other versions
WO2006018311A2 (en
Inventor
Bob Turner
Ian P Thrippleton
Original Assignee
Seapro Theranostics Internat
Bob Turner
Ian P Thrippleton
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Seapro Theranostics Internat, Bob Turner, Ian P Thrippleton filed Critical Seapro Theranostics Internat
Priority to EP05776219A priority Critical patent/EP1778870A2/en
Publication of WO2006018311A2 publication Critical patent/WO2006018311A2/en
Publication of WO2006018311A3 publication Critical patent/WO2006018311A3/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • H—ELECTRICITY
    • H05—ELECTRIC TECHNIQUES NOT OTHERWISE PROVIDED FOR
    • H05B—ELECTRIC HEATING; ELECTRIC LIGHT SOURCES NOT OTHERWISE PROVIDED FOR; CIRCUIT ARRANGEMENTS FOR ELECTRIC LIGHT SOURCES, IN GENERAL
    • H05B6/00—Heating by electric, magnetic or electromagnetic fields
    • H05B6/64—Heating using microwaves
    • H05B6/80—Apparatus for specific applications
    • H05B6/806—Apparatus for specific applications for laboratory use
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0689—Sealing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0822—Slides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/44—Sample treatment involving radiation, e.g. heat

Abstract

The present invention relates to processes and devices for determining a nucleic acid analyte in a biological sample by hybridisation, particularly by fluorescence in situ hybridisation (FISH), wherein the sample is treated with microwaves.
PCT/EP2005/008992 2004-08-19 2005-08-19 Determination of nucleic acid analytes by treatment with microwaves WO2006018311A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP05776219A EP1778870A2 (en) 2004-08-19 2005-08-19 Determination of nucleic acid analytes by treatment with microwaves

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP04019741 2004-08-19
EP04019741.0 2004-08-19

Publications (2)

Publication Number Publication Date
WO2006018311A2 WO2006018311A2 (en) 2006-02-23
WO2006018311A3 true WO2006018311A3 (en) 2006-04-20

Family

ID=34926236

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2005/008992 WO2006018311A2 (en) 2004-08-19 2005-08-19 Determination of nucleic acid analytes by treatment with microwaves

Country Status (2)

Country Link
EP (1) EP1778870A2 (en)
WO (1) WO2006018311A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9714943B2 (en) * 2010-12-30 2017-07-25 University Of South Florida Methods and materials for capture antibody targeted fluorescent in-situ hybridization (CAT-FISH)
DE102015214414B4 (en) * 2015-07-29 2020-10-22 Berthold Technologies Gmbh & Co. Kg Method and system for determining biological properties of samples

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995015671A1 (en) * 1993-12-03 1995-06-08 Inceltec Ltd Apparatus and process for performing a chemical reaction
US20020115201A1 (en) * 1999-09-16 2002-08-22 Barenburg Barbara Foley Microfluidic devices with monolithic microwave integrated circuits
WO2003093407A1 (en) * 2002-05-01 2003-11-13 Bio-Molecular Holdings Pty Limited Device for the amplification of dna, comprising a microwave energy source

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995015671A1 (en) * 1993-12-03 1995-06-08 Inceltec Ltd Apparatus and process for performing a chemical reaction
US20020115201A1 (en) * 1999-09-16 2002-08-22 Barenburg Barbara Foley Microfluidic devices with monolithic microwave integrated circuits
WO2003093407A1 (en) * 2002-05-01 2003-11-13 Bio-Molecular Holdings Pty Limited Device for the amplification of dna, comprising a microwave energy source

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
BEIMFOHR C ET AL: "IN SITU IDENTIFICATION OF LACTOCOCCI ENTEROCOCCI AND STREPTOCOCCI", SYSTEMATIC AND APPLIED MICROBIOLOGY, XX, XX, vol. 16, no. 3, November 1993 (1993-11-01), pages 450 - 456, XP000982317, ISSN: 0723-2020 *
BROETZ HEIKE ET AL: "New insights into the mechanism of action of lantibiotics: Diverse biological effects by binding to the same molecular target", JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, vol. 46, no. 1, July 2000 (2000-07-01), pages 1 - 6, XP002303204, ISSN: 0305-7453 *
CHIN S -F ET AL: "A simple and reliable pretreatment protocol facilitates fluorescent in situ hybridisation on tissue microarrays of paraffin wax embedded tumour samples.", MOLECULAR PATHOLOGY, vol. 56, no. 5, October 2003 (2003-10-01), pages 275 - 279, XP002303201, ISSN: 1366-8714 *
DE MEULEMEESTER M ET AL: "The application of microwave denaturation in comparative genomic hybridization", GENETIC ANALYSIS: BIOMOLECULAR ENGINEERING, ELSEVIER SCIENCE PUBLISHING, US, vol. 13, no. 5, 1 November 1996 (1996-11-01), pages 129 - 133, XP004063527, ISSN: 1050-3862 *
DON R H ET AL: "'TOUCHDOWN?' PCR TO CIRCUMVENT SPURIOUS PRIMING DURUNG GENE AMPLIFICATION", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 19, no. 14, 1991, pages 4008, XP002003542, ISSN: 0305-1048 *
EVANS C ET AL: "A NOTE ON THE USE OF MICROWAVES IN AN IMPROVED NON-RADIOACTIVE DNA HYBRIDIZATION PROCEDURE FOR THE DETECTION OF BACTERIA IN FOODSTUFFS", LETTERS IN APPLIED MICROBIOLOGY, vol. 10, no. 6, 1990, pages 233 - 236, XP009038988, ISSN: 0266-8254 *
GOSALVEZ J ET AL: "FISHing in the microwave: The easy way to preserve proteins. I. Colocalization of DNA probes and surface antigens in human leukocytes", CHROMOSOME RESEARCH, vol. 10, no. 2, 2002, pages 137 - 143, XP002303202, ISSN: 0967-3849 *
SHU GUOPING G ET AL: "Detection of gene expression patterns in various plant tissues using non-radioactive mRNA in situ hybridization", WORLD WIDE WEB JOURNAL OF BIOLOGY, vol. 4, no. 7, 17 April 2000 (2000-04-17), XP002364109, Retrieved from the Internet <URL:http://www.epress.com/w3jbio/vol4/shu/index.html> [retrieved on 20060124] *

Also Published As

Publication number Publication date
EP1778870A2 (en) 2007-05-02
WO2006018311A2 (en) 2006-02-23

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