WO2005118849A1 - Method for stabilizing assay reagents, reagent container with stabilized assay reagents and use thereof - Google Patents
Method for stabilizing assay reagents, reagent container with stabilized assay reagents and use thereof Download PDFInfo
- Publication number
- WO2005118849A1 WO2005118849A1 PCT/FI2005/050194 FI2005050194W WO2005118849A1 WO 2005118849 A1 WO2005118849 A1 WO 2005118849A1 FI 2005050194 W FI2005050194 W FI 2005050194W WO 2005118849 A1 WO2005118849 A1 WO 2005118849A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- reagent
- pair
- reagents
- assay
- onto
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
- B01L3/50851—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates specially adapted for heating or cooling samples
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/16—Reagents, handling or storing thereof
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0829—Multi-well plates; Microtitration plates
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/16—Surface properties and coatings
Definitions
- the composition of assay reagents may be suitable for performing a qualitative or quantitative assay for an analyte that is a) a reactant in or b) an inhibitor of, or c) an activator of, or d) protein, or e) an enzyme capable of catalyzing a chemical reaction that takes place as a result of combining a sample containing said analyte with the composition of assay reagents.
- a substrate for an enzyme can be separated from at least one of the other assay reagents, at least one of the other assay reagents being, in a preferable embodiment, an enzyme capable of acting upon said substrate.
- FIG. 1 shows the instability of PCR reagents dried as a mixture.
- the figure shows the europium signal-to-noise ratios (on the y-axis) obtained at PCR cycles 21-43 (on the x-axis) measured from PCR reactions that were prepared using predried PCR reagents that had been dried as mixtures containing all PCR components, including the DNA polymerase.
- PTC 1 filled triangles
- PTC 2 filled diamonds
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Description
Claims
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US11/628,354 US7972838B2 (en) | 2004-06-04 | 2005-06-03 | Method for stabilizing assay reagents, reagent container with stabilized assay reagents and use thereof |
JP2007513990A JP4732450B2 (en) | 2004-06-04 | 2005-06-03 | Method for stabilizing assay reagent, reagent container containing stabilized assay reagent and use thereof |
DE602005010432T DE602005010432D1 (en) | 2004-06-04 | 2005-06-03 | PROCESS FOR STABILIZING TEST RAGENTS, RND USE THEREOF |
EP05746855A EP1766055B1 (en) | 2004-06-04 | 2005-06-03 | Method for stabilizing assay reagents, reagent container with stabilized assay reagents and use thereof |
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US57682004P | 2004-06-04 | 2004-06-04 | |
FI20040768 | 2004-06-04 | ||
US60/576,820 | 2004-06-04 | ||
FI20040768A FI20040768A0 (en) | 2004-06-04 | 2004-06-04 | Method for stabilizing assay reagents, reagent tank containing stabilized assay reagents and its use |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2005118849A1 true WO2005118849A1 (en) | 2005-12-15 |
Family
ID=32524448
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/FI2005/050194 WO2005118849A1 (en) | 2004-06-04 | 2005-06-03 | Method for stabilizing assay reagents, reagent container with stabilized assay reagents and use thereof |
Country Status (7)
Country | Link |
---|---|
US (1) | US7972838B2 (en) |
EP (1) | EP1766055B1 (en) |
JP (1) | JP4732450B2 (en) |
AT (1) | ATE411401T1 (en) |
ES (1) | ES2314664T3 (en) |
FI (1) | FI20040768A0 (en) |
WO (1) | WO2005118849A1 (en) |
Cited By (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN100386628C (en) * | 2006-04-21 | 2008-05-07 | 中国科学院武汉病毒研究所 | Method of detecting etiology by tacteriophage immunity PCR |
EP2128614A1 (en) * | 2008-05-14 | 2009-12-02 | Samsung Electronics Co., Ltd. | Microfluidic device containing lyophilized reagent therein and analyzing method using the same |
WO2010144683A3 (en) * | 2009-06-12 | 2011-02-03 | Micronics, Inc. | Compositions and methods for dehydrated storage of on-board reagents in microfluidic devices |
WO2012010708A1 (en) * | 2010-07-23 | 2012-01-26 | Aj Innuscreen Gmbh | Method, device and test kit for molecular-biological reactions |
CN103276087A (en) * | 2013-05-30 | 2013-09-04 | 杭州金溪生物技术有限公司 | High-sensitivity protein detection method |
US8835146B2 (en) | 2009-06-12 | 2014-09-16 | Micronics, Inc. | Rehydratable matrices for dry storage of TAQ polymerase in a microfluidic device |
US9096894B2 (en) | 2011-05-26 | 2015-08-04 | Arkray, Inc. | Dry reagent, dry reagent kit, reagent container, and method for producing dry reagent |
US10087440B2 (en) | 2013-05-07 | 2018-10-02 | Micronics, Inc. | Device for preparation and analysis of nucleic acids |
US10190153B2 (en) | 2013-05-07 | 2019-01-29 | Micronics, Inc. | Methods for preparation of nucleic acid-containing samples using clay minerals and alkaline solutions |
US10386377B2 (en) | 2013-05-07 | 2019-08-20 | Micronics, Inc. | Microfluidic devices and methods for performing serum separation and blood cross-matching |
US10436713B2 (en) | 2012-12-21 | 2019-10-08 | Micronics, Inc. | Portable fluorescence detection system and microassay cartridge |
US10518262B2 (en) | 2012-12-21 | 2019-12-31 | Perkinelmer Health Sciences, Inc. | Low elasticity films for microfluidic use |
EP3607092A4 (en) * | 2018-01-12 | 2020-12-09 | Stem Arts Projects, LLC | Reaction condition composition for circularizing oligonucleotide probes |
Families Citing this family (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20080050737A1 (en) * | 2006-05-23 | 2008-02-28 | Boaz Arieli | Ambient Temperature Stable Kits for Molecular Diagnostics |
JP5209990B2 (en) * | 2008-02-29 | 2013-06-12 | 栄研化学株式会社 | Reaction and / or detection container, and reaction and / or detection kit including the same |
JP5315079B2 (en) * | 2009-02-13 | 2013-10-16 | 大陽日酸株式会社 | Enzyme reaction reagent, enzyme reaction reagent kit, and method for storing solution for enzyme reaction |
WO2013010333A1 (en) * | 2011-07-21 | 2013-01-24 | 瑞基海洋生物科技股份有限公司 | Method of reverse transcription-polymerase chain reaction |
CN103849548A (en) | 2012-12-03 | 2014-06-11 | 三星电子株式会社 | Reagent container for amplifying nucleic acid, method of preparing the reagent container, method of storing the reagent, and microfluidic system for nucleic acid analysis |
EP2998738B1 (en) * | 2013-05-13 | 2020-02-12 | Konica Minolta, Inc. | Cell-staining method and specimen collection tube for use in method |
WO2016106113A1 (en) * | 2014-12-23 | 2016-06-30 | Ge Healthcare Uk Limited | Methods and reagents for reverse-transcription polymerase chain reaction |
WO2016106111A1 (en) | 2014-12-23 | 2016-06-30 | Ge Healthcare Uk Limited | Methods and reagents for reverse-transcription polymerase chain reaction |
US10822645B1 (en) | 2014-12-23 | 2020-11-03 | Global Life Sciences Solutions Operations UK Ltd | Methods and reagents for reverse-transcription polymerase chain reaction |
ES2973471T3 (en) | 2016-04-22 | 2024-06-20 | Becton Dickinson Co | Multiplexed polymeric dye device and methods to use the same cross-reference with related applications |
JP7058662B2 (en) | 2017-02-08 | 2022-04-22 | ベクトン・ディキンソン・アンド・カンパニー | Dry dye reagent device, and how to manufacture and use it |
US11992844B2 (en) * | 2018-11-13 | 2024-05-28 | Becton, Dickinson And Company | Dried reagent strainers and methods for making and using the same |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2674253A1 (en) * | 1991-03-19 | 1992-09-25 | Diagnostics Pasteur | Freeze-dried composition for the multiplication of nucleic acid sequences |
EP0726310A1 (en) * | 1995-02-10 | 1996-08-14 | Gen-Probe Incorporated | Stabilized enzyme compositons for nucleic acid amplification |
EP0834729A2 (en) * | 1996-09-26 | 1998-04-08 | Becton, Dickinson and Company | DNA microwell device and method |
WO2001092569A2 (en) * | 2000-05-31 | 2001-12-06 | Tepnel Medical Limited | Formulation for polymerase chain reaction and vessel containing same |
Family Cites Families (10)
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DE2333434C3 (en) * | 1973-06-30 | 1978-04-06 | Istvan D. Dr. 5024 Pulheim Bartos | Process for carrying out serological tests according to the principle of the complement fixation reaction and ready-to-use rapid test pack for this |
US4351158A (en) * | 1980-01-22 | 1982-09-28 | American Home Products Corporation | Method of producing multicomponent lyophilized product |
EP1248112A3 (en) * | 1987-04-27 | 2004-08-25 | Inverness Medical Switzerland GmbH | Immunochromatographic specific binding assay device |
US5229297A (en) * | 1989-02-03 | 1993-07-20 | Eastman Kodak Company | Containment cuvette for PCR and method of use |
CA2143365A1 (en) | 1994-03-14 | 1995-09-15 | Hugh V. Cottingham | Nucleic acid amplification method and apparatus |
WO1997010056A2 (en) * | 1995-09-12 | 1997-03-20 | Becton Dickinson And Company | Device and method for dna amplification and assay |
US6429026B1 (en) * | 1996-04-08 | 2002-08-06 | Innotrac Diagnostics Oy | One-step all-in-one dry reagent immunoassay |
US6558901B1 (en) * | 1997-05-02 | 2003-05-06 | Biomerieux Vitek | Nucleic acid assays |
CA2400207A1 (en) * | 2000-02-18 | 2001-08-23 | Aclara Biosciences, Inc. | Multiple-site reaction device and method |
US7238538B2 (en) * | 2003-09-19 | 2007-07-03 | Freitag Helmut E | Chromatographic assay device and methods |
-
2004
- 2004-06-04 FI FI20040768A patent/FI20040768A0/en not_active Application Discontinuation
-
2005
- 2005-06-03 ES ES05746855T patent/ES2314664T3/en active Active
- 2005-06-03 JP JP2007513990A patent/JP4732450B2/en active Active
- 2005-06-03 WO PCT/FI2005/050194 patent/WO2005118849A1/en active Application Filing
- 2005-06-03 AT AT05746855T patent/ATE411401T1/en not_active IP Right Cessation
- 2005-06-03 US US11/628,354 patent/US7972838B2/en active Active
- 2005-06-03 EP EP05746855A patent/EP1766055B1/en active Active
Patent Citations (5)
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FR2674253A1 (en) * | 1991-03-19 | 1992-09-25 | Diagnostics Pasteur | Freeze-dried composition for the multiplication of nucleic acid sequences |
EP0726310A1 (en) * | 1995-02-10 | 1996-08-14 | Gen-Probe Incorporated | Stabilized enzyme compositons for nucleic acid amplification |
US5834254A (en) * | 1995-02-10 | 1998-11-10 | Gen-Probe Incorporated | Stabilized enzyme compositions for nucleic acid amplification |
EP0834729A2 (en) * | 1996-09-26 | 1998-04-08 | Becton, Dickinson and Company | DNA microwell device and method |
WO2001092569A2 (en) * | 2000-05-31 | 2001-12-06 | Tepnel Medical Limited | Formulation for polymerase chain reaction and vessel containing same |
Non-Patent Citations (1)
Title |
---|
NURMI JUSSI ET AL: "High-throughput genetic analysis using time-resolved fluorometry and closed-tube detection", ANALYTICAL BIOCHEMISTRY, vol. 299, no. 2, 15 December 2001 (2001-12-15), pages 211 - 217, XP002345041, ISSN: 0003-2697 * |
Cited By (18)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN100386628C (en) * | 2006-04-21 | 2008-05-07 | 中国科学院武汉病毒研究所 | Method of detecting etiology by tacteriophage immunity PCR |
EP2128614A1 (en) * | 2008-05-14 | 2009-12-02 | Samsung Electronics Co., Ltd. | Microfluidic device containing lyophilized reagent therein and analyzing method using the same |
WO2010144683A3 (en) * | 2009-06-12 | 2011-02-03 | Micronics, Inc. | Compositions and methods for dehydrated storage of on-board reagents in microfluidic devices |
US8835146B2 (en) | 2009-06-12 | 2014-09-16 | Micronics, Inc. | Rehydratable matrices for dry storage of TAQ polymerase in a microfluidic device |
US8921085B2 (en) | 2009-06-12 | 2014-12-30 | Micronics, Inc. | Compositions and methods for dehydrated storage of on-board reagents in microfluidic devices |
US9938571B2 (en) | 2009-06-12 | 2018-04-10 | Micronics, Inc. | Compositions and methods for dehydrated storage of on-board reagents in microfluidic devices |
WO2012010708A1 (en) * | 2010-07-23 | 2012-01-26 | Aj Innuscreen Gmbh | Method, device and test kit for molecular-biological reactions |
US9096894B2 (en) | 2011-05-26 | 2015-08-04 | Arkray, Inc. | Dry reagent, dry reagent kit, reagent container, and method for producing dry reagent |
US11181105B2 (en) | 2012-12-21 | 2021-11-23 | Perkinelmer Health Sciences, Inc. | Low elasticity films for microfluidic use |
US10518262B2 (en) | 2012-12-21 | 2019-12-31 | Perkinelmer Health Sciences, Inc. | Low elasticity films for microfluidic use |
US10436713B2 (en) | 2012-12-21 | 2019-10-08 | Micronics, Inc. | Portable fluorescence detection system and microassay cartridge |
US10386377B2 (en) | 2013-05-07 | 2019-08-20 | Micronics, Inc. | Microfluidic devices and methods for performing serum separation and blood cross-matching |
US10190153B2 (en) | 2013-05-07 | 2019-01-29 | Micronics, Inc. | Methods for preparation of nucleic acid-containing samples using clay minerals and alkaline solutions |
US10087440B2 (en) | 2013-05-07 | 2018-10-02 | Micronics, Inc. | Device for preparation and analysis of nucleic acids |
US11016108B2 (en) | 2013-05-07 | 2021-05-25 | Perkinelmer Health Sciences, Inc. | Microfluidic devices and methods for performing serum separation and blood cross-matching |
CN103276087B (en) * | 2013-05-30 | 2015-03-18 | 杭州金溪生物技术有限公司 | High-sensitivity protein detection method |
CN103276087A (en) * | 2013-05-30 | 2013-09-04 | 杭州金溪生物技术有限公司 | High-sensitivity protein detection method |
EP3607092A4 (en) * | 2018-01-12 | 2020-12-09 | Stem Arts Projects, LLC | Reaction condition composition for circularizing oligonucleotide probes |
Also Published As
Publication number | Publication date |
---|---|
ATE411401T1 (en) | 2008-10-15 |
EP1766055B1 (en) | 2008-10-15 |
ES2314664T3 (en) | 2009-03-16 |
JP2008501331A (en) | 2008-01-24 |
EP1766055A1 (en) | 2007-03-28 |
US7972838B2 (en) | 2011-07-05 |
US20070243601A1 (en) | 2007-10-18 |
JP4732450B2 (en) | 2011-07-27 |
FI20040768A0 (en) | 2004-06-04 |
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