WO2005094802A2 - Inhibiting proliferation of neoplastic cells by mlk inhibition - Google Patents
Inhibiting proliferation of neoplastic cells by mlk inhibition Download PDFInfo
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- WO2005094802A2 WO2005094802A2 PCT/US2005/008682 US2005008682W WO2005094802A2 WO 2005094802 A2 WO2005094802 A2 WO 2005094802A2 US 2005008682 W US2005008682 W US 2005008682W WO 2005094802 A2 WO2005094802 A2 WO 2005094802A2
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- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/535—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with at least one nitrogen and one oxygen as the ring hetero atoms, e.g. 1,2-oxazines
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- A—HUMAN NECESSITIES
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Definitions
- the present invention relates generally to the fields of medicine and cell biology. Specifically, the present invention relates to methods of preventing proliferation of cancer cells and of reducing toxicity of an anticancer compound during a cancer therapy. More specifically, the present invention relates to methods of inhibiting an activity of a multi-lineage kinase protein to prevent proliferation of cancer cells.
- Serine/threonine protein kinases belonging to the mixed-lineage kinase (MLK) family, control the activity of mitogen-activated protein (MAP) kinases and are involved primarily in regulating tissue development and apoptotic responses
- the MLK family includes MLK1-4, the dual leucine zipper-bearing kinase
- MLK proteins each contain highly conserved structural motifs that are important for protein interactions and signal transduction including a Src homology 3 (SH3) domain, a catalytic domain, two leucine zipper (LeuZip) motifs, and a Cdc42/Rac interactive binding (CRLB) motif (1).
- SH3 Src homology 3
- LeuZip leucine zipper
- CRLB Cdc42/Rac interactive binding
- MLK proteins are activated by upstream G-proteins including Cdc42 and Racl, which recruit cytoplasmic MLK proteins to the plasma membrane by interacting with the CRIB motif (3). MLK protein activity may also be regulated through auto-inhibition involving intramolecular interactions between the SH3 domain and a single proline residue between the CRIB domain and the leucine zipper region (4).
- HSP90 heat shock protein-90
- MLK3 is a potent activator of the c-Jun N-terminal kinase (JNK) MAP kinases by directly phosphorylating and activating the JNK kinases, MKK4 and MKK7 (9- 10). MLK3 may also activate the p38 MAP kinases through direct phosphorylation of MKK3 and MKK6, which are the primary p38 MAP kinase activators (10).
- MLK proteins have been proposed to play a critical role in the progression of neurodegenerative diseases, such as Parkinson's, Huntington's, and Alzheimer's diseases, through a mechanism involving MLK induced JNK activation, cyctochrome c release, and caspase activation of apoptotic pathways (6,8).
- MLK induced JNK activation e.g., IL-12 induced JNK activation
- cyctochrome c release e.g., apoptotic pathways
- caspase activation of apoptotic pathways e.g., apoptotic pathways.
- MLK proteins may also function in promoting cell proliferation.
- MLK3 MLK-like protein
- the present invention fulfills this long-standing need and desire in the art. SUMMARY OF THE INVENTION The present invention is directed to a method of inhibiting proliferation of a neoplastic cell.
- the method comprises contacting the neoplastic cell with a compound that selectively inhibits an activity of a mixed-lineage kinase (MLK). Inhibition of a MLK activity thereby inhibits proliferation of the neoplastic cell.
- the present invention is directed to a related method of inhibiting proliferation of a neoplastic cell.
- the neoplastic cell is contacted with a compound that selectively inhibits an activity of mixed lineage kinase (MLK).
- the mixed lineage kinase has a sequence selected from the group consisting of SEQ ID NO:l, SEQ ID NO:2, SEQ ID NO:3, and SEQ ID NO:4, where the inhibition of the MLK activity inhibits proliferation of the neoplasm.
- the present invention also is directed to a method of treating a cancer in a subject.
- the method comprises administering an inhibitor that selectively binds to a mixed-lineage kinase (MLK) or polypeptide thereof and inhibiting an activity of the MLK or MLK polypeptide upon binding the inhibitor to arrest proliferation of cancer cells. Arresting proliferation of the cancer cells treats the cancer.
- the method may comprise a further step of administering an anticancer drug to the individual.
- the present invention is directed to a related method of reducing toxicity of a cancer therapy in an individual in need thereof.
- the method comprises co-administering to the individual an inhibitor that selectively binds to a mixed- lineage kinase (MLK) or polypeptide thereof and an anticancer drug.
- the dosage of the anticancer drug administered with the inhibitor is lower than a dosage required when the anticancer drug is administered singly. Toxicity of the cancer therapy to the individual is thereby reduced.
- the present invention is directed further to a method of screening for a compound to inhibit mixed lineage kinase (MLK) activity and to arrest proliferation of a neoplastic cell.
- MLK mixed lineage kinase
- the level of an activity of a MLK protein or of a polypeptide thereof or polypeptide fragment having the MLK activity is measured in the presence or absence of the compound. A decrease in the level of MLK activity in the presence of the compound compared with the level of MLK activity in the absence of the compound is indicative that the compound has an ability to inhibit MLK activity.
- a culture of neoplastic cells having an activated MLK activity is contacted with the compound having an ability to inhibit the MLK activity.
- the amount of cell proliferation of the neoplastic cells in the presence of the inhibitory compound with the amount of cell proliferation of the neoplastic cells in the absence of the inhibitory compound is compared.
- a decrease in cell proliferation in the presence of the inhibitory compound compared to cell proliferation in the absence of the inhibitory compound is indicative that the inhibitory compound has the ability to prevent cell proliferation.
- the present invention is directed further still to compounds identified by the screening methods described herein. These compounds inhibit an activity of a mixed lineage kinase or polypeptide thereof and arrest proliferation of neoplastic cells.
- These compounds may be used in any of the methods of inhibiting cell proliferation of a neoplastic cell, of treating a cancer or of reducing toxicity of an anticancer drug described in the present invention. Additional embodiments of the invention are directed to the use of a compound that selectively inhibits an activity of a mixed-lineage kinase (MLK) in methods of inhibiting proliferation of neoplastic cells, including cancer cells, and/or in the treatment of cancer, and in the preparation of medicaments and pharmaceutical compositions for same.
- MLK mixed-lineage kinase
- Figure 1A shows HeLa or MH 3T3 cells grown in the absence (closed squares or circles) or presence (open squares or circles) of CEP-11004 (500 nM) for up to 72 hours. The number of cells was counted at time 0 and after 24, 48, or 72 hours in each condition.
- Figure IB shows trypsinized A549 cells (1000 per treatment) plated in the presence of varying doses of CEP-11004. After 14 days, the resulting colonies were stained with crystal violet, and counted. Similar results were obtained with HeLa and SUM152 breast cancer cells.
- Figures 2A-2D demonstrate that CEP-11004 causes G2/M-phase arrest in transformed cells.
- HeLa Figure 2A
- HEK293 Figure 2B
- Figure 2C Figure 2C
- Figure 2D MRC5 cells were treated in the absence (control) or presence of CEP-11004 (500 nM) for 15 hours and 2N or 4N DNA content in cells was measured by FACS analysis following propidium iodide staining.
- Figures 3A-3D demonstrate that CEP-11004 treatment of synchronized cells during late S-phase/early G2-phase causes G2/M-phase arrest.
- HeLa cells were synchronized at the Gl/S-phase boundary by double thymidine block, released back into the cell cycle, and treated 5 hours after release in the absence (Figure 3A) or presence (Figure 3B) of CEP-11004 (500 nM).
- FIG. 4A-4E demonstrate that CEP-11004 targets MLK3 but not DLK or MKK1 activity.
- HeLa cells were transfected with HA- tagged MLK3 wild type (WT) or a catalytically inactive MLK3 (KR) mutant.
- the MLK3 wild type transfected cells were incubated with or without CEP-11004 (500 nM) for 4 hours prior to harvesting. Wild type and inactive MLK3 were immunoprecipitated with a MLK3 antibody and incubated with MBP as a substrate in an in vitro kinase assay.
- the graph shows the relative amount of phosphate incorporation into MBP under each condition and the immunoblot shows the amount of the MLK3 proteins in the immunoprecipitates.
- HeLa cells were transfected with HA-tagged JNK1 plus MLK3 wild type or Flag-tagged DLK and then treated in the absence or presence of CEP-11004 (500 nM) for 4 hours prior to harvesting.
- Immunoblots for active JNK (pJNK), HA-JNK1, Flag-DLK, or HA-MLK3 are shown in the top to bottom panels, respectively.
- HeLa cells were transfected with wild type (WT) MLK3, inactive (KR) MLK3, or a constitutively active MKK1 mutant (CA) and then treated with or without CEP-11004 (500 nM).
- WT wild type
- KR inactive
- CA constitutively active MKK1 mutant
- CEP-11004 500 nM
- the relative level of active ERK was determined by immunoblotting with a phospho-specific ERK1/2 (ppERK)
- FIG. 4D HeLa cells were co-transfected with HA-JNK1 and GFP tagged MLK2 or HA-MLK3. Transfected cells were then treated for 1 hour with various concentrations of CEP-11004 and JNK activity was determined by immunoblotting with a phospho-specific JNK1/2 antibody (pJNK, upper panel). The expression of HA-JNK1, GFP-MLK2, or HA-MLK3 is shown in the lower panels. The upper and lower arrows in the HA-MLK3 blot indicate phosphorylated and de- phosphorylated forms of MLK3, respectively.
- Figure 4E is a graph of the relative JNK activity as quantified by measuring the ratio of pJNK to total JNK by densitometry in cells expressing MLK2 (closed squares) or MLK3 (open squares).
- Figures 5A-5B demonstrate that CEP-11004 causes cell cycle arrest in pro-metaphase. HeLa cells grown on coverslips were synchronized at the Gl/S boundary with excess thymidine and released back into the cell cycle. At 5 hours after release, cells were treated in the absence or presence of CEP-11004 (500 nM) for an additional 4 or 6 hours (9 or 11 hours cumulative time after Gl/S release).
- FIG 5A the percentage of cells in prophase (P), pro-metaphase (PM), metaphase (M), or anaphase/telophase (AT) was determined by DAPI staining of chromosomes in control (open bars) or CEP-11004 treated (closed bars) cells.
- the left and right graphs represent the percentage of mitotic cells 9 and 11 hours, respectively, after Gl/S-phase release. Data represent the average and standard error from 3 independent experiments.
- Figure 5B the number of cells in pro-metaphase were counted at 11 hours after Gl/S-phase release following treatment with increasing concentrations of CEP-11004 (added at 5 hours after Gl/S-phase release). 300-350 cells were counted at each concentration.
- Figures 6A-6C demonstrate that exogenous MLK3 inhibits CEP- 11004-induced mitotic arrest.
- HeLa cells were co-transfected with pEGFP in the presence or absence (mock) of HA-tagged MLK3 wild type and then synchronized by double thymidine block.
- the cells were treated with various concentrations of CEP-11004 (75, 100, 200 nM) for 8 hours and then harvested (13 hours post Gl/S release).
- Figure 6A shows immunoblots for cyclin BI
- top panel MLK3 (middle panel), and ⁇ -actin (lower panel) as a protein loading
- FIG. 6B synchronized HeLa cells transfected with pEGFP plus or minus MLK3 were grown on coverslips, treated with or without various concentrations of CEP-11004, fixed at 13 hours after release from Gl/S, and stained with DAPI. The number of mitotic cells (prophase, pro-metaphase, metaphase, and anaphase/telophase cells) was determined by DAPI staining in GFP positive cells (closed bars) or GFP positive cells that co-express MLK3 (open bars).
- Figure 6C shows relative Cdc2 kinase activity in lysates collected 13 hours after Gl/S release in cells that were transfected with pEGFP alone or pEGFP and MLK3.
- FIG. 7A-7B demonstrate that CEP-11004 delays nuclear histone H3 phosphorylation in early prophase. HeLa cells grown on coverslips were synchronized at Gl/S with excess thymidine and released back into the cell cycle. At 5 hours after release, cells were incubated in the absence or presence of CEP-11004 (500 nM).
- a method of inhibiting proliferation of a neoplastic cell comprising contacting the neoplastic cell with a compound that selectively inhibits an activity of a mixed-lineage kinase (MLK), thereby inhibiting proliferation of the neoplastic cell.
- the neoplastic cell may comprise a cancer.
- a cancer are a breast cancer, a lung cancer, a cervical cancer, a pancreatic cancer, a bladder cancer, a colon cancer, or any cancer having a Ras mutation.
- the compound may be an indolocarbazole molecule.
- Representative examples are CEP-11004 or CEP-1347.
- the mixed lineage kinase may be a MLK1 polypeptide having the sequence shown in SEQ ID NO:3.
- the mixed lineage kinase may be a MLK2 protein having the sequence shown in SEQ ID NO:4.
- the mixed lineage kinase may be a MLK3 protein having the sequence shown in SEQ ID NO:l or in SEQ ID NO:2.
- a method of inhibiting proliferation of a neoplastic cell comprising contacting the neoplastic cell with a compound that selectively inhibits an activity of mixed lineage kinase (MLK) having a sequence selected from the group consisting of SEQ ID NO:l, SEQ J-D NO:2, SEQ ID NO:3, and SEQ ID NO:4, where the inhibition of the MLK activity inhibits proliferation of the neoplasm.
- MLK mixed lineage kinase
- the neoplastic cell may comprise a cancer as described supra. Additionally, in all aspects the inhibitor is as described supra.
- a method of treating a cancer in a subject comprising administering an inhibitor that selectively binds to a mixed-lineage kinase (MLK) or polypeptide thereof; inhibiting an activity of the MLK or MLK polypeptide upon binding said inhibitor such that proliferation of cancer cells is arrested, thereby treating the cancer in the subject.
- the method may comprise administering an anticancer drug to the subject.
- the anticancer drug may be administered concurrently or sequentially with the MLK inhibitor.
- a dosage of the anticancer drug is lower than a dosage required when the anticancer drug is administered singly, thereby reducing toxicity of the anticancer drug to the individual.
- anticancer drugs are cisplatin, oxaliplatin, carboplatin, doxorubicin, a camptothecin, paclitaxel, methotrexate, vinblastine, etoposide, docetaxel hydroxyurea, celecoxib, fluorouracil, busulfan, imatinib mesylate, alembuzumab, aldesleukin, and cyclophosphamide. Additionally, in all aspects the types of cancer, MLK inhibitors and the MLK proteins and polypeptides thereof are as described supra.
- the present invention provides a method of reducing toxicity of a cancer therapy in an individual in need thereof, comprising administering to the individual an inhibitor that selectively binds to a mixed-lineage kinase (MLK) or polypeptide thereof and an anticancer drug, wherein a dosage of the anticancer drug administered with the inhibitor is lower than a dosage required when the anticancer drug is administered singly, thereby reducing toxicity of the cancer therapy to the individual.
- the anticancer drug may be administered concurrently or sequentially with the MLK inhibitor.
- the MLK inhibitors, the MLK proteins and polypeptides, the anticancer drugs and the types of cancer are as described supra.
- a method of screening for a compound to inhibit mixed lineage kinase (MLK) activity and to arrest proliferation of a neoplastic cell comprising a) measuring the level of an activity of a MLK protein or of a polypeptide thereof or polypeptide fragment having said MLK activity in the presence or absence of the compound; b) comparing the level of MLK activity in the presence of the compound with the level of MLK activity in the absence of the compound, wherein a decrease in MLK activity in the presence of the compound is indicative that the compound has an ability to inhibit MLK activity; c) contacting a culture of the neoplastic cells having an activated MLK activity with the compound having an ability to inhibit the MLK activity; and d) comparing the amount of cell proliferation of the neoplastic cells in the presence of the inhibitory compound with the amount of cell proliferation of the neoplastic cells in the absence of the inhibitory compound, wherein a decrease in cell proliferation in the presence of the compound
- the mixed lineage kinase is a MLK2 protein or a MLK3 protein or a MLK1 polypeptide or a polypeptide fragment having an MLK activity.
- the MLK1 polypeptide may have the sequence shown in SEQ ID NO:3.
- the MLK2 polypeptide may have the sequence shown in SEQ ID NO:4.
- the MLK3 polypeptide may have the sequence shown in SEQ J-D NO:l or in SEQ ID NO:2.
- the MLK polypeptide fragment having the MLK activity may comprise about 700, 650, 600, 550, 500, 450, 400, 350, 300, 250, 200, 150, or 100 continuous amino acids of the MLK2 or MLK3 proteins or of the MLK1 polypeptide.
- neoplastic cell and the types of cancer are as described supra.
- a compound identified by the methods of screening for an inhibitor of a mixed lineage kinase activity and of cell proliferation of a neoplastic cell are used herein.
- the term, "a” or “an” may mean one or more.
- the words “a” or “an” when used in conjunction with the word “comprising”, the words “a” or “an” may mean one or more than one.
- another may mean at least a second or more.
- the term “contacting” refers to any suitable method of bringing an inhibitory agent into contact with a MLK protein or polypeptide or fragment thereof, as described, or a cell comprising the same. In vitro or ex vivo this is achieved by exposing the MLK protein or polypeptide or fragment thereof or cells comprising the same to the inhibitory agent in a suitable medium. For in vivo applications, any known method of administration is suitable as described herein.
- Neoplasm refers to a mass of tissue or cells characterized by, inter alia, abnormal cell proliferation.
- the abnormal cell proliferation results in growth of these tissues or cells that exceeds and is uncoordinated with that of the normal tissues or cells and persists in the same excessive manner after the stimuli which evoked the change ceases or is removed.
- Neoplastic tissues or cells show a lack of structural organization and coordination relative to normal tissues or cells which usually results in a mass of tissues or cells which can be either benign or malignant.
- cancer refers to a malignant neoplasm.
- the term “transformed” or the phrase “transformed cell” refers to a cell that exhibits neoplastic growth.
- the term “treating” or the phrase “treating a cancer” or “treating a neoplasm” includes, but is not limited to, halting the growth of the neoplasm or cancer, killing the neoplasm or cancer, or reducing the size of the neoplasm or cancer. Halting the growth refers to halting any increase in the size or the number of or size of the neoplastic or cancer cells or to halting the division of the neoplasm or the cancer cells.
- Reducing the size refers to reducing the size of the neoplasm or the cancer or the number of or size of the neoplastic or cancer cells.
- the term “subject” refers to any target of the treatment.
- the term “MLK inhibitor” means a molecular entity of natural, semi-synthetic or synthetic origin that blocks, stops, inhibits, and/or suppresses an activity of a mixed lineage kinase (MLK) polypeptide, including, but not limited to, a MLK1, a MLK2 and/or a MLK3 polypeptide.
- MLK polypeptide As used herein, the term "MLK polypeptide” is used interchangeably with “MLK protein.”
- the inhibition of the human MLK3 (SEQ ID NO:l) polypeptide is a non-limiting exemplary embodiment and that the inhibition of a MLK3 polypeptide in a different mammal, such as a mouse (SEQ ID NO:2), and/or the human MLK2 polypeptide (SEQ ID NO:4) and/or the human MLK1 (SEQ ID NO:3) polypeptide are contemplated as within the spirit and scope of the invention.
- the present invention discloses that MLK proteins or polypeptides serve as an important regulator of microtubule formation during mitosis in transformed cells exhibiting neoplastic growth.
- MLK proteins or polypeptides blocks mitotic progression in a pro-metaphase like arrest due to the inability to properly form a mitotic spindle necessary for metaphase to anaphase transitions.
- Inhibition of MLK proteins may preferentially inhibit mitotic transitions and cell proliferation of transformed cells, but not in normal cells.
- the inherent abnormalities of cell cycle regulation in the transformed cells may make these cells more sensitive to undergo cell cycle arrest and apoptosis in response to MLK inhibitors.
- MLK proteins or polypeptides serve as a unique target for treatment of cancer cell proliferation without affecting normal cell function.
- MLK polypeptides as a unique target for treating a neoplasm, e.g., but not limited to, a cancer, such that neoplastic cell proliferation is arrested, stopped, blocked, or ceases to occur without affecting normal untransformed cell function.
- the present invention is drawn to MLK inhibitors that bind specific polypeptide sequences of an MLK protein and inhibit or interfere with an activity thereof to affect cancer cell proliferation.
- the MLK inhibitor may block, stop, inhibit, and or suppress an activity of an MLK polypeptide, that is, but not limited to, a full-length polypeptide such as SEQ ID NOS: 1, 2 and/or 4 or a peptide fragment thereof, such as SEQ ID NO:3.
- a polypeptide fragment also may comprise about 700, 650, 600, 550, 500, 450, 400, 350, 300, 250, 200, 150, or 100 continuous amino acids of the MLK polypeptide, including a MLK1 polypeptide of SEQ ID NO:3, a MLK2 polypeptide of SEQ ID NO: 4 or a MLK3 polypeptide of SEQ ID NOS: 1 or 2.
- the MLK inhibitor may be an indolocarbazole compound or a derivative or analog thereof or other molecule effective to interfere with an activity of an MLK protein or polypeptide, such as a kinase activity.
- Representative inhibitors are CEP-11004 or CEP-1347. It is contemplated that CEP-11004 selectively binds to MLK3.
- potential compounds may be screened for their ability to inhibit an activity of a MLK protein or polypeptide and to inhibit cell proliferation of a transformed cell.
- a cancer cell culture having activated MLK activity is contacted with a potential MLK inhibitor.
- a decrease in cell proliferation, as compared to control, may be determined by standard assays, such as trypan blue exclusion or a colony formation assay.
- Compounds effective to inhibit an activity of a MLK protein or polypeptide and to inhibit cell proliferation may be screened further by FACS analysis of DNA content to characterize the nature of the decrease in cell proliferation.
- potential inhibitors of MLKs may be screened initially by contacting the potential inhibitor with one or more MLK proteins or polypeptides described herein in the presence of a known substrate for an MLK-associated activity.
- the MLK-associated activity may be assayed in the presence of ATP and a substrate phosphorylated via MLK activity and in the presence or absence of the potential MLK inhibitor.
- a decrease in MLK activity in the presence of the potential inhibitor compared to activity in the absence of the potential inhibitor is indicative that it has an ability to inhibit an MLK activity.
- Such enzyme assays are known and standard in the art.
- the MLK polypeptide may be any of MLKl-4 or may be a fragment of thereof, as described, provided that the polypeptide fragments retain at least one activity associated with the MLKl-4 polypeptide. Subsequently, any potential inhibitor of the MLK-associated activity may be used in the cell proliferation assays as described.
- Potential inhibitors of cell proliferation of cancer cells may be natural, semi-synthetic or synthetic compounds known to inhibit or interfere with an activity of an MLK protein or may be compounds screened from chemical libraries or may be a synthetic derivative or analog compound having a structure similar to a known inhibitor. Inhibitors of MLKs identified by the methods described herein can block proliferation of cancer cells without affecting normal cell proliferation.
- Such inhibitors may be used to inhibit proliferation of neoplastic cells, to treat a cancer or to reduce the toxicity of a cancer drug to normal cells.
- the MLK inhibitors may be used to treat any subject, preferably a mammal, more preferably a human, having a pathophysiological condition characterized by the presence of transformed cells, e.g., a neoplasm, such as, but not limited, to a cancer.
- a cancer may be a breast cancer, a lung cancer, a cervical cancer, a pancreatic cancer, a bladder cancer, a colon cancer, or another cancer having a Ras mutation.
- Administration of an MLK inhibitor to a subject results in growth arrest of cancer cells without affecting the growth of a normal cell.
- the MLK inhibitors of the present invention may be used to inhibit proliferation of non- malignant neoplastic diseases and disorders.
- An anticancer drug may be administered concurrently or sequentially with the MLK inhibitor.
- the effect of co-administration with an MLK inhibitor is to lower the dosage of the anticancer drug normally required that is known to have at least a minimal pharmacological or therapeutic effect against a cancer or cancer cell, for example, the dosage required to eliminate a cancer cell.
- MLK inhibitors and anticancer drugs can be administered independently, either systemically or locally, by any method standard in the art, for example, subcutaneously, intravenously, parenterally, intraperitoneally, intradermally, intramuscularly, topically, enterally, rectally, nasally, buccally, vaginally or by inhalation spray, by drug pump or contained within transdermal patch or an implant.
- Dosage formulations of MLK inhibitors may comprise conventional non-toxic, physiologically or pharmaceutically acceptable carriers or vehicles suitable for the method of administration.
- the MLK inhibitors and anticancer drugs or pharmaceutical compositions thereof may be administered independently one or more times to achieve, maintain or improve upon a therapeutic effect. It is well within the skill of an artisan to determine dosage or whether a suitable dosage of either or both of the MLK inhibitor and anticancer drug comprises a single administered dose or multiple administered doses. An appropriate dosage depends on the subject's health, the progression or remission of the cancer, the route of administration and the formulation used.
- the following examples are given for the purpose of illustrating various embodiments of the invention and are not meant to limit the present invention in any fashion.
- EXAMPLE 1 Cell culture and reagents HeLa, MH 3T3, HEK293, A549, or MRC-5 cells were grown in a DMEM supplemented with 10% fetal bovine serum (FBS) and penicillin (100 U/ml) and streptomycin (100 ⁇ g/ml).
- cells were transfected with cDNA (1 ⁇ g) for pEGFP (BD Biosciences/Clontech, Palo Alto, CA), hemagglutinin (HA)-tagged MLK3, kinase dead mutant MLK3 (MLK3 K144R), GFP-tagged MLK2 (kindly provided by Dr. Donna Dorow), Flag-tagged DLK, or HA-tagged JNK1 using Lipofectamine® (Invitrogen, Carlsbad, CA). Transfected cells were harvested 16-24 hours after transfection.
- pEGFP BD Biosciences/Clontech, Palo Alto, CA
- HA hemagglutinin
- MLK3 kinase dead mutant MLK3
- GFP-tagged MLK2 (kindly provided by Dr. Donna Dorow)
- Flag-tagged DLK or HA-tagged JNK1 using Lipofectamine® (Invitrogen, Carlsbad, CA).
- Antibodies specific for MLK3 (C-20), JNK (C-17), phospho-JNKl/2 (G-7), Cyclin BI (GNS1), MKK2 (C-16), and Cdc2 (#17) were purchased from Santa Cruz Biotech (Santa Cruz, CA).
- Antibodies for p62 nucleoporin (N43620) and phospho-specific histone H3 (serine 10) (Cat# 07-081) were purchased from BD Biosciences (Palo Alto, CA) and Upstate Biotechnology (Charlottesville, VA), respectively.
- CEP-11004 (kindly provided by Cephalon Inc. West Chester, PA) was reconstituted at a stock concentration of 0.4 mM in DMSO.
- cells were synchronized by double thymidine block as previously described (18-19). Briefly, cells were incubated for 16 hours with 2 mM thymidine in 10% FBS containing DMEM. The excess thymidine was washed off with Hanks Buffered Saline Solution (HBSS) and cells were released back into the cell cycle with complete medium. After 8 hours, cells were treated a second time with 2 mM thymidine in 10% FBS containing DMEM for another 16 hours to arrest cells at the Gl/S-phase boundary. Synchronized cells were then washed with HBSS and released back into the cell cycle and harvested at various time points after Gl/S release for analysis.
- HBSS Hanks Buffered Saline Solution
- Proteins were transferred to PVDF membrane (Perkin Elmer Life Sciences; Boston, MA), blocked for 1-2 hours with 5 % nonfat dry milk in Tris-buffered saline (TBS) (50 mM Tris-base, pH 7.4, 0.15 M NaCl, and 0.1 % Tween-20) and incubated with the primary antibodies in TBS containing 1% BSA solution for 1 to 16 hours. Membranes were washed several times in TBS-Tween solution and incubated with HRP conjugated anti-mouse or anti-rabbit antibodies (O.l ⁇ g/ml). Immunoreactivity was detected by enhanced chemiluminescence (ECL; Amersham, Buckinghamshire, England). In some experiments, proteins were immunoprecipitated with 1 ⁇ g/ml
- p62 nucleoporin followed by incubation with fluorescein or Texas Red conjugated secondary antibodies and counterstaining for cellular DNA with 4',6-diamidino-2- phenylindole (DAPI, 0.2 ⁇ g/ml in PBS).
- DAPI 4',6-diamidino-2- phenylindole
- Cells were identified using Nikon E800 Epi- fluorescence microscope (Image Systems, Columbia, MD) and captured with a Hamamatsu CCD camera. Cell images were processed using J-Plab software (Scanalytics, Fairfax, VA).
- MBP basic protein
- Cdc2 kinase activity Cdc2 was immunoprecipitated from cell lysates by incubating with
- Mitotic index assay Cells grown on coverslips were synchronized at the Gl/S-phase boundary as described in Examples 4-5. In some cases, MLK3 was co-expressed with the pEGFP vector in order to identify transfected cells. At 5 hours after release from thymidine-induced Gl/S-phase block, cells were incubated in the presence or absence of varying concentrations of CEP-11004. At varying times after release, coverslips were fixed and cellular DNA was stained with DAPI. GFP-positive mitotic cells in prophase, prometaphase, metaphase, anaphase, or telophase were identified, counted, and expressed as a fraction of the total cells counted to determine the mitotic index.
- CEP-11004 caused a dose dependent inhibition of A549 airway epithelia carcinoma cell colony formation. A similar inhibition of colony formation was observed in HeLa cells and ER negative SUM159 breast cancer cells treated with CEP-11004 (data not shown).
- FACS analysis of DNA content was used to determine the effects of CEP-11004 on cell cycle progression. As shown, HeLa or HEK293 cells treated with CEP-11004 for 20 hours accumulated 4N DNA indicative of arrest in G2 or M-phase of the cell cycle (Figs. 2A-2B).
- CEP-11004 inhibits MLK3. but not other related kinases
- the specificity of CEP-11004 for inhibition of MLK proteins and not other related kinases was examined. These experiments focused on the MLK3 isoform. MLK3 is expressed ubiquitously and is characterized the best of the MLK proteins. Also, although MLK2 shares more than 70% sequence identify in the kinase catalytic domain and other regions with MLK3, MLK2 expression is restricted to brain, skeletal muscle, and testis (21). Additionally, the full sequence of MLK1 has yet to be identified. Finally, MLK3 has recently been implicated to be involved in G2/M-phase transitions (15). The ability for CEP-11004 to block MLK3 activity was tested first.
- HA-tagged MLK3 wild type was overexpressed in HeLa cells and treated with or without CEP-11004 for 4 hours before harvesting.
- CEP- 11004 treatment significantly inhibited expressed MLK3 activity compared to untreated cells.
- CEP-11004 on a related mixed lineage kinase, dual leucine zipper kinase (DLK) was measured in transfected CHO cells.
- DLK dual leucine zipper kinase
- MLK3 has higher activity ( ⁇ 4 fold) during G2 and M-phase transitions as compared to Gl/S phase and this activity was blocked by CEP-11004 (data not shown).
- Overexpression of MLK3 has been shown to induce ERK activation through MEK1 (13). Therefore, the effect of CEP-11004 on ERK activation following overexpression of MLK3 wild type or a constitutively active MKKl mutant was examined. Although both MLK3 and active MKKl stimulate ERK activity, only MLK3-induced ERK activation was inhibited by CEP-11004 (Fig. 4C). Lastly, the ability for CEP-11004 to inhibit MLK2 and MLK3 proteins was compared.
- MLK3 induced JNKl phosphorylation was inhibited by 80% with 100 nM CEP-11004, significant inhibition of MLK2 activation of JNKl required greater than 400 nM CEP-11004 (Fig. 4D and E). This is consistent with CEP-11004 having approximately 3 fold higher specificity towards MLK3 as compared to MLK2 (11). Thus, these data support MLK3 as the major MLK isoform targeted by CEP-11004.
- CEP-11004 does not affect the activity of other MLK-related (DLK) or non-MLK related protein kinases (MKKl). Nevertheless, others have suggested that the effects of MLK inhibitors on Ras- induced proliferation of NIH 3T3 cells may be due to simultaneous inhibition of the p21-activated kinase-1 (Pakl) (20). Pakl activity was not tested in the presence or the absenceof CEP-11004 because the doses that were used to initiate cell cycle arrest herein were approximately 10 fold less than the reported IC 5 o concentrations needed for the MLK inhibitor to block Pakl activity (20). It is contemplated that mitotic arrest occurs at concentrations as low as 100-200 nM (Figs. 6A-6C).
- CEP-1347 Another MLK inhibitor, CEP-1347, which has similar biological properties to CEP-11004 was reported to activate the ERK pathway (12). However, these experiments were done in neuronal cells and no evidence that CEP-11004 causes ERK activation in non-neuronal cells has been found. In contrast, as demonstrated, CEP-11004 treatment inhibited MLK3 induced ERK activation (Fig. 4C), which is consistent with a role for MLK3 in activating the ERK pathway in tumor cells (14). This further supports the opposing roles for MLK3 in promoting proliferation of non-neuronal tumor cells but cell death of neuronal cells.
- CEP-11004 induces mitotic arrest in pro-metaphase Since it did not appear that CEP-11004 affected progression into mitosis (Figs. 3A-3D), the nature of CEP-11004 induced M-phase arrest was characterized by examining the various stages of mitosis in synchronized cells treated in the presence or absence of CEP-11004.
- Gl/S-phase cells grown on coverslips were released back into the cell cycle for 5 hours, treated with or without 400 nM CEP- 11004 for an additional 4 or 6 hours incubation, i.e., 9 and 11 hours, respectively, after release from Gl/S block and the DNA was stained with DAPI. Chromosome morphology was examined by fluorescence microscopy and the percentage of cells in prophase, pro-metaphase, metaphase, or anaphase/telophase was determined. At 9 hours after Gl/S, CEP-11004 treated cells began to show accumulation of pro- metaphase cells compared to untreated cells (Fig. 5A, left graph). Furthermore, cells in metaphase or anaphase/telophase were not evident in CEP-11004 treated cells (Fig.
- MLK3 overexpression reverses the effect of CEP-11004 on mitotic arrest
- MLK3 was a major target of CEP- 11004
- HeLa cells were co-transfected with pEGFP, to identify transfected cells, in the presence of control vector or HA-MLK3 and then were synchronized at the Gl/S boundary using excess thymidine. At 5 hours after Gl/S release, cells were incubated with various concentrations of CEP-11004 for an additional 8 hours.
- Mitotic progression was examined by immunoblotting for cyclin BI, by determining the mitotic index and by assaying Cdc2 kinase activity. Cyclin BI levels were elevated in mock transfected cells treated with 100 or 200 nM CEP- 11004, which is consistent with a mitotic arrest (Fig. 6A). In contrast, MLK3 transfected cells had lower cyclin BI levels after CEP-1104 treatment consistent with the ability for exogenous MLK3 to prevent the mitotic arrest induced by these concentrations of CEP-11004 (Fig. 6A). Next, the mitotic index was determined by DAPI staining in mock or
- CEP-11004 delays histone H3 phosphorylation in early mitosis
- the mechanisms of CEP- 11004 induced mitotic arrest were further examined by monitoring mitosis-specific phosphorylation events. Phosphorylation of histone H3 at serine 10 is commonly used as a marker of mitotic progression (22-23).
- HeLa cells were treated with or without CEP- 11004 at 5 hours after release from Gl/S-phase thymidine block and allowed to incubate for an additional 4 hours. Cells were then fixed and processed for immunofluorescence of cyclin BI and phospho- histone H3 (pH3) to identify the mitotic cells.
- MLK3 might localize to the nucleus of mitotic cells (Fig. 7B) and support a direct phosphorylation of histone H3 by MLK3. Consistent with this is the finding that MLK3 is related to NLMA, a mitotic kinase that phosphorylates S10 on histone H3 in vitro (28). Even though MLK3 can activate ERK and p38 signaling pathways and histone H3 phosphorylation at serine 10 may be mediated by ERK and p38 MAP kinases in response to stress stimuli (29), it is yet to be determined whether these kinases contribute to histone phosphorylation during early mitosis prior to NEB.
- aurora A and B kinases are potential candidate kinases that regulate phosphorylation of histone H3 at S10 during mitosis.
- aurora B is the most likely physiological histone H3 kinase since aurora A localizes primarily to the centrosome (32). Nonetheless, aurora B depletion does not eliminate S10 phosphorylation on histone H3 indicating the involvement of other mitotic kinases (27).
- aurora kinases are regulated by phosphorylation, there is no evidence to link MLK proteins with regulation of the aurora kinases.
- DAPI staining in CEP-11004 was highly disorganized (Fig. 9A). Greater than 60% of the mitotic cells contained the aberrant microtubule organization (Fig. 9B). Examination of interphase cells treated for 4 hours with CEP-11004 showed no apparent effects on microtubules as compared to untreated cells (data not shown). Thus, the inability for CEP-11004 treated cells to progress through metaphase is likely due to defects in microtubule organization and aberrant spindle formation. Although overexpression of MLK3 was suggested recently to promote microtubule instability (15), it is not clear what effects inhibition of MLK3 activity has on microtubule organization in mitotic cells.
- MLK-regulated microtubule associated proteins are being affected.
- Expression of ectopic MLK3 was shown to destabilize microtubules in HEK293 cells through a mechanism that does not involve JNK or p38 MAP kinase activation (15). However, it is not clear whether this observation is unique to MLK3 or an over-expression artifact.
- MLK2 and active JNK have also been shown to co- localize along microtubules and interact with motor proteins (24). Whether these interactions were occurring in a cell cycle-dependent manner was not determined.
- MLK3 may have additional substrates that may help explain MLK3 functions during the cell cycle that are independent of downstream MAP kinase activation. It is contemplated that MLK3 inhibition by CEP-11004 prevents cell proliferation by disrupting microtubule events necessary for mitotic progression. This may at first appear to contrast with a previous study that cited unpublished data indicating that down-regulation of MLK3 using RNA interference (RNAi) does not affect cell proliferation or DNA content by flow cytometry (15).
- RNAi RNA interference
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NHEU T V ET AL: "THE K252A DERIVATIVES, INHIBITORS FOR THE PAK/MLP KINASE FAMILY, SELECTIVELY BLOCK THE GROWTH OF RAS TRANSFORMANTS" CANCER JOURNAL, JONES AND BARTLETT PUBLISHERS, US, vol. 8, no. 4, July 2002 (2002-07), pages 328-336, XP009035466 ISSN: 1528-9117 * |
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