WO2005058913A1

WO2005058913A1 - Pyrido- and pyrimidopyrimidine derivatives as anti- proliferative agents

Info

Publication number: WO2005058913A1
Application number: PCT/EP2004/053501
Authority: WO
Inventors: Eddy Jean Edgard Freyne; Marc Willems; Pierre Henri Storck; Virginie Sophie Poncelet; Kristof Van Emelen; Peter Jacobus Johannes Antonius Buijnsters; Werner Constant Johan Embrechts; Timothy Pietro Suren Perera
Original assignee: Janssen Pharmaceutica N.V.
Priority date: 2003-12-18
Filing date: 2004-12-15
Publication date: 2005-06-30
Also published as: EA013904B1; DE602004012891D1; DE602004012891T2; HK1099293A1; NO337701B1; EP1697384A1; IL176357A0; AR046957A1; NZ547794A; KR101174672B1; MXPA06007017A; EA200601177A1; AU2004298448A1; EP1697384B1; CA2549869C; US8772272B2; AU2004298448B2; JP4936897B2; US20070078132A1; KR20060126716A

Abstract

The present invention concerns the compounds of formula (I) the N-oxide forms, the pharmaceutically acceptable addition salts and the stereochemically isomeric forms thereof, wherein a1-a2=a3-a4 represents a divalent radical selected from N-CH=CH-CH, N-CH N-CH or CH-CH=N-CH; Z represents NH; Y represents -C3-9alkyl-, -Cl-5alkyl-NR13-Cl-5alkyl-,-C1-6alkyl-NH-CO- or -CO-NH -C1-6alkyl- ; X1 represents -O- or -NR11-; X2 represents -C1-2alkyl-, -O-C1-2alkyl, -O- or -O-CH2-;R1 represents hydrogen or halo; R2 represents hydrogen, cyano, halo, hydroxycarbonyl-Cl-4 alkyloxycarbonyl-, Het16-carbonyl- or Ar5; R3 represents hydrogen; R4 represents hydroxy, C1-4alkyloxy-, Ar4-C1-4alkyloxy or R4 represents Cl-4alkyloxy substituted with one or where possible two or more substituents selected from C1-4alkyloxy- or Het2-; R11 represents hydrogen; R12 represents hydrogen, Cl-4alkyl- or Cl-4alkyl-oxy-carbonyl-; R13 represents Het14-Cl-4alkyl, in particular morpholinyl-Cl-4alkyl; Het2 represents a heterocycle selected from morpholinyl or piperidinyl optionally substituted with C1-4alkyl-, preferably methyl; Het14 represents morpholinyl; Het16 represents a heterocycle selected from morpholinyl or pyrrolidinyl; Ar4 represents phenyl; Ar5 represents phenyl optionally substituted with cyano.

Description

PYRIDO- AND PYRIMIDOPYRIMIDINE DERIVATIVES AS ANTI- PROLIFERATIVE AGENTS

This invention relates to py imidopyrimidine derived macrocycles that have been found to possess anti-proliferative activity, such as anti-cancer activity and are accordingly useful in methods of treatment of the human or animal body, for example in the manufacture of medicaments for use in hyper proliferative disorders such as atherosclerosis, restenosis and cancer. The invention also relates to processes for the manufacture of said pyrimidopyrimidine derivatives, to pharmaceutical compositions containing them and to their use in the manufacture of medicaments of use in the production of anti-proliferative effect . In particular, the compounds of the present invention were found to inhibit tyrosine kinase enzymes, also called tyrosine kinases. Tyrosine kinases are a class of enzymes, which catalyse the transfer of the terminal phosphate of adenosine triphosphate to the phenolic hydroxyl group of a tyrosine residue present in the target protein. It is known, that several oncogenes, involved in the transformation of a cell into a malignant tumour cell, encode tyrosine kinase enzymes including certain growth factor receptors such as EGF, FGF, IGF-1R, IR, PDGF and VEGF. This family of receptor tyrosine kinases and in particular the EGF family of receptor tyrosine kinases are frequently present in common human cancers such as breast cancer, non-small cell lung cεncers including adenocarcinomas and squamous cell cancer of the lung, bladder cancer, oesophageal cancer, gastrointestinal cancer such as colon, rectal or stomach cancer, cancer of the prostate, leukaemia and ovarian, bronchial or pancreatic cancer, which are examples of cell proliferation related disorders.

Accordingly, it has been recognised that the selective inhibition of tyrosine kinases will be of value in the treatment of cell proliferation related disorders. Support for this view is provided by the development of Herceptin® (Trastuzumab) and Gleevec™ (imatinib mesylate) the first examples oftarget based cancer drugs. Herceptin^® (Trastuzumab) is targeted against ΕLetl/neu, a receptor tyrosine kinase found to be amplified up to 100-fold in about 30% of patients with invasive breast cancer. In clinical trials Herceptin^® (Trastuzumab) proved to have anti-tumour activity against breast cancer (Review by L.K. Shawer et al, "Smart Drugs: Tyrosine kinase inhibitors in cancer therapy", 2002, Cancer Cell Vol.1, 117), and accordingly provided the proofofprinciple for therapy targeted to receptor lyrosine kinases. The second example, Gleevec™ (imatinib mesylate), is targeted against the abelson tyrosine kinase (BcR-Abl), a constitutively active cytoplasmic tyrosine kinase present in virtually all patients with chronic myelogenous leukaemia (CML) and 15% to 30% of adult patients with acute lymphoblastic leukaemia. In clinical trials Gleevec™ (imatinib mesylate) showed a spectacular efficacy with minimal side effects that led to an approval within 3 months of submission. The speed of passage of this agent through clinical trials and regulatory review has become a case study in rapid drug development (Drucker BJ. & Lydon N., "Lessons learned from the development of an Abl tyrosine kinase inhibitor for chronic myelogenous leukaemia.", 2000, J.Clin.Invest. 105, 3). Further support is given by the demonstration that EGF receptor tyrosine kinase inhibitors, specifically attenuates the growth in athymic nude mice of transplanted carcinomas such as human mammary carcinoma or human squamous cell carcinoma (Review by T.R. Burke Jr., Drugs of the Future, 1992, 17, 119). As a consequence, there has been considerable interest in the development of drugs to treat different cancers that target the EGFR receptor. For example, several antibodies that bind to the extra-cellular domain of EGFR are undergoing clinical trials, including Erbitux™ (also called C225, Cetuximab), which was developed by Imclone Systems and is in Phase HI clinical trials for the treatment of several cancers. Also, several promising orally active drugs that are potent and relatively specific inhibitors of the EGFR tyrosine kinase are now well advanced in clinical trials. The AstraZeneca compound ZD1839, which is now called IRESSA^® and approved for the treatment of advanced non-small-cell lung cancer, and the OSI/Genentech/Roche compound OSI-774, which is now called Tarceva™ (erlotinib) , have shown marked efficacy against several cancers in human clinical trials (Morin M. J., "From oncogene to drug: development of small molecule tyrosine kinase inhibitors as anti-tumour and anti-angiogenic agents, 2000, Oncogene 19, 6574).

In addition to the above, EGF receptor tyrosine kinases has been shown to be implicated in non-malignant proliferative disorders such as psoriasis (elder et al, Science, 1989, 243; 811). It is therefore expected that inhibitors of EGF type receptor tyrosine kinases will be useful in the treatment of non-malignant diseases of excessive cellular proliferation such as psoriasis, benign prostatic hypertrophy, atherosclerosis and restenosis. It is disclosed in International Patent Applications WO 96/07657 & WO97/32880 that pyrimidopyrimidines are useful as inhibitors of tyrosine kinase and in particular of the EGF type receptor tyrosine kinases. Unexpectedly it was found that pyrimidopyrimidine derivatives of the present formula (I) that are different in structure show to have tyrosine kinase inhibitory activity.

It is accordingly an object of the present invention to provide further tyrosine kinase inhibitors useful in the manufacture of medicaments in the treatment of cell proliferative related disorders.

This invention concerns compounds of formula (I)

the N-oxide forms, the pharmaceutically acceptable addition salts and the stereochemically isomeric forms thereof, wherein

a^x-a²=a³-a⁴ represents a divalent radical selected from Ν-CH=CH-CH, N-CH=N-CH or CH-CH=N-CH; Z represents O, NH or S; Y represents -C_3-9alkyl-, -C_3- alkenyl-, -Ci-salkyl-oxy- -salkyl-, -Ci-salkyl-NR^-Ci.salkyl-^Ci.saJJkyl-NR^-CO-Ci.salkyl-,

-Ci-ealkyl-NH-CO-, -CO-NH-Ci-ealkyl-, -NH-CO-C_1-6alkyl-, -CO-Cι_-7alkyl-, -C_1-7alkyl-CO-,

X¹ represents a direct bond, O, -O-Cι_-2alkyl-, CO, -CO- Cι_-2alkyl-, NR¹¹, -NR¹¹-C_1-2alkyl-, NR¹⁶-CO-, NR¹⁶-CO-Ci_-2alkyl-, -O-N=CH- or Cι_-2alkyl; X² represents a direct bond, O, -O-Cι_-2alkyl-, CO, -CO- Cι_-2alkyl-, NR¹², NR¹²-C_1-2alkyl-, NR¹⁷-CO-, NR¹⁷-CO-Cι_-2alkyl-, Het²⁰-Cι_-2alkyl-, -O-N=CH- or Cι_-2alkyl; R¹ represents hydrogen, cyano, halo, hydroxy, formyl, Cι-6alkoxy-, C^aUcyl-, Ci-βalkoxy- substituted with halo, Ci^alkyl substituted with one or where possible two or more substituents selecte<l from hydroxy or halo; R² represents hydrogen, cyano, halo, hydroxy, hydroxycarbonyl-, Het¹⁶-carbonyl-,

aminocarbonyl-, mono-or

Het¹, formyl,

C_2-6alkynyl-, Gj-βcycloalkyl-, C_3-6cycloalkyloxy-, Ci-βalkoxy-, Ar⁵, Ar^x-oxy-, dihydroxyborane , Ci-βalkoxy- substituted with halo, substituted with one or where possible two or more substituents selected! from halo, hydroxy or NR⁵R⁶,

wherein said

is optionally substituted with one or where possible two or more substituents selected from hydroxy or Cwalkyl-oxy-; R³ represents hydrogen,

cyano or C_1-4alkyl substituted with one or more substituents selected from halo,

amino-, mono-or di(Ci a!kyl)an ino-,

or phenyl;

R⁴ represents hydrogen, hydroxy, Ar'-oxy, Ar⁴-Cι_-4alkyloxy-,

C_2-4alkenyloxy- optionally substituted with Het¹² or R⁴ represents ^alkyloxy substituted with one or where possible two or more substituents selected from

R⁵ and R⁶ are each independently selected from hydrogen or

R⁷ andR⁸ are each independently selected from hydrogen, Ci^alkyl, Het⁸, arninosulfonyl-, mono- or di

'

hydroxycarbonyl-Cι.4alkyl-, C₃-₆Cycloalkyl, Het⁹- Het¹⁰-carbonyl-, polyhydroxy-C_1- alkyl-, Het¹¹-Cι- alkyl- o«r

R⁹ and R¹⁰ are each independently selected from hydrogen,

C_3-6cycloalkyl,

R¹¹ represents hydrogen,

optionally substituted with Het^CwaJJ laminocarbonyl-, C_2-4alkenylsulfonyl-, C₁- alkyloxyC_1- alkyl- or phenyl optionally substituted with one or where possible two or more substituents selected from hydrogen, hydroxy, amino or

R¹² represents hydrogen,

Het¹⁷,

C_2- alkenylcarbonyl- optionally substituted with

C_2-4alkenylsulfonyl-,

or phenyl optionally substituted wiCh one or where possible two or more substituents selected from hydrogen, hydroxy, amino or

R¹³ represents hydrogen,

Het¹³,

or phenyl optionally substituted with one or where possible two or more substituents selected from hydrogen, hydroxy, amino or C_1-4alkyloxy-; R¹⁴ andR¹⁵ are each independently selected from hydrogen,

R¹⁶ andR¹⁷ are each independently selected from hydrogen, C_1-4alkyl,

Het¹ represents a heterocycle selected from piperidinyl, morpholinyl, piperazinyl, furanyl, pyrazolyl, dioxolanyl, thiazolyl, oxazolyl, i idazolyl, isoxazolyl, oxadiazolyl, pyridinyl or pyrrolidinyl wherein said Het¹ is optionally substituted with one or where possible two or more substituents selected from amino, C_1-4alkyl,

phenyl,

mono- or

or amino-carbonyl-; Het² represents a heterocycle selected from moφholinyl, piperazinyl, piperidinyl, pyrrolidinyl, thiomoφholinyl or dithianyl wherein said Het² is optionally substituted with one or where possible two or more substituents selected from hydroxy, halo, amino,

aminoC_Malkyl-, mono- or

sulfonyl-, aminosulfonyl-;

Het³, Het⁴ and Het⁸ each independently represent a heterocycle selected from moφholinyl, piperazinyl, piperidinyl, furanyl, pyrazolyl, dioxolanyl, thiazolyl, oxazolyl, imidazolyl, isoxazolyl, oxadiazolyl, pyridinyl or pyrrolidinyl wherein said Het³, Het⁴ or Het⁸ is optionally substituted with one or where possible two or more substituents selected from hydroxy-, amino-, C_1-4alkyl-, aminosulfonyl-, mono- or di(C_1- alkyl)arninosulfonyl or

Het⁵ represent a heterocycle selected from pyrrolidinyl or piperidinyl wherein said heterocycle is optionally substituted with one or where possible two or more substituents selected from

C_3-6cycloalkyl,

Het⁶ and Het⁷ each independently represent a heterocycle selected from moφholinyl, pyrrolidinyl, piperazinyl or piperidinyl wherein said Het⁶ or Het⁷ is optionally substituted with one or where possible two or more substituents selected from Ca-ecycloalkyl, hydroxy-C_1-4alkyl-,

or

Het⁹ and Het¹⁰ each independently represent a heterocycle selected from furanyl, piperidinyl, moφholinyl, piperazinyl, pyrazolyl, dioxolanyl, thiazolyl, oxazolyl, imidazolyl, isoxazolyl, oxadiazolyl, pyridinyl or pyrrolidinyl wherein said Her⁹ or Het¹⁰ is optionally substituted

C3-6cycloalkyl-C_1- alkyl- or

Het¹

represents a heterocycle selected from indolyl or

Het¹² represents a heterocycle selected from moφholinyl, piperazinyl, piperidinyl, pyrrolidinyl, thiomoφholinyl or dithianyl wherein said Het¹² is optionally substituted with one or where possible two or more substituents selected from hydroxy, halo, amino,

hydroxy-Cι_₄alkyl-oxy-Cι- aIkyl-, mono- or di(Cι^alkyl)amino- or mono- or

Het¹³ represent a heterocycle selected from pyrrolidinyl or piperidinyl wherein said heterocycle is optionally substituted with one or where possible two or more substituents selected from C_1- alkyl, C_3-6cycloalkyl, hydroxy-C_1- allkyl-,

Het¹⁴ represent a heterocycle selected from moφholinyl, pyrrolidinyl, piperazinyl or piperidinyl wherein said heterocycle is optionally substituted with one or where possible two or more substituents selected from C_1- alkyl, C_3-6cycloalkyl,

Het¹⁵ and Het each independently represent a heterocycle selected from moφholinyl, pyrrolidinyl, piperazinyl or piperidinyl wherein said Het¹⁵ or Het²¹ are optionally substituted with one or where possible two or more substituents selected from Ci. alkyl, C_3-6cycloalkyl,

or polyhydroxy-

Het¹⁶ represent a heterocycle selected from moφholinyL pyrrolidinyl, piperazinyl, 1,3,2-dioxaborolane or piperidinyl wherein said heterocycle is optionally substituted with one or more substituents selected from

Het¹⁷ represent a heterocycle selected from pyrrolidinyl or piperidinyl wherein said heterocycle is optionally substituted with one or where possible two or more substituents selected from

C₃-6cycloalkyl, hydroxy-C_1-4alkyl-, C_Malkylo yC alkyl

Het¹⁸ and Het¹⁹ each independently represent a heterocycle selected from moφholinyl, pyrrolidinyl, piperazinyl or piperidinyl wherein said Het¹⁸ and Het¹⁹ are optionally substituted with one or where possible two or more substituents selected from C₃_₆cycloalkyl, hydroxy-Cι-₄alkyl-,

or

Het²⁰ represents a heterocycle selected from pyrrolidinyl, 2-pyrrolidinyl, piperidinyl, piperazinyl or pyrazoUdinyl wherein said heterocycle is optionally substituted with one or where possible two or more substituents selected from C_1- alkyl, C_3-6Cycloalkyl,

or polyhydroxy-Ci^alkyl-; and Ar¹, Ar², Ar³, Ar⁴ and Ar⁵ each independently represent phenyl optionally substituted with cyano,

aminosulfonylamino-, hydroxy-C_1- alkyl, aminosulfonyl-, hydroxy-,

As used in the foregoing definitions and hereinafter,

- halo is generic to fluoro, chloro, bromo and iodo; - C^alkyl defines methyl or ethyl;

- C_j^al yl defines straight and branched chain saturated hydrocarbon radicals having from 1 to 3 carbon atoms such as, for example, methyl, ethyl, propyl and the like;

- C_j^alkyl defines straight and branched chain saturated hydrocarbon radicals having from 1 to 4 carbon atoms such as, for example, methyl, ethyl, propyl, butyl, 1- methylethyl, 2-methylpropyl, 2,2-dimethylethyl and the like;

- C_j^alkyl defines straight and branched chain saturated hydrocarbon radicals having from 1 to 5 carbon atoms such as, for example, methyl, ethyl, propyl, buyl pentyl, 1- methylbutyl, 2,2-dimethylpropyl, 2,2-dimethylethyl and the like;

- C^alkyl is meant to include Ci-salkyl and the higher homologues thereof having 6 carbon atoms such as, for example hexyl, 1,2-dimethylbutyl, 2-methylpentyl and the like;

- C_j^al yl is meant to include Ci-βalkyl and the higher homologues the eof having 7 carbon atoms such as, for example 1,2,3-dimethylbutyl, 1, 2-methylpentyl and the like;

- C_3-9alkyl defines straight and branched chain saturated hydrocarbon radicals having from 3 to 9 carbon atoms such as propyl, butyl, pentyl, hexyl, heptyl, octyl, nonyl and the like;

- C^alkenyl defines straight and branched chain hydrocarbon radicals containing one double bond and having from 2 to 4 carbon atoms such as, for example vinyl, 2- propenyl, 3 -butenyl, 2-butenyl and the like; - C_3-9alkenyl defines straight and branched chain hydrocarbon radicals containing one double bond and having from 3 to 9 carbon atoms such as, for example 2-propenyl, 3- butenyl, 2-butenyl, 2-pentenyl, 3-pentenyl, 3-methyl-2-butenyl, 3-hexenyl and the like; - C₂-6alkynyl defines straight and branched chain hydrocarbon radicals containing one triple bond and having from 2 to 6 carbon atoms such as, for example, 2-propynyl, 3- butynyl, 2-butynyl, 2-pentynyl, 3-pentynyl, 3-methyl-2-butynyl, 3-hexynyl and the like;

- C₃-6cycloalkyl is generic to cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl; -

defines straight or branched saturated hydrocarbon radicals such as methoxy, ethoxy, propyloxy, butyloxy, 1-methylethyloxy, 2-methylpropyloxy and the like;

- Ci-βalkyloxy is meant to include C_1-4alkyloxy and the higher homologues such as methoxy, ethoxy, propyloxy, butyloxy, 1-methylethyloxy, 2-methylpropyloxy and the like;

-

as defined hereinbefore, having two, three or were possible more hydroxy substituents, such as for example trifluoromethyl.

As used in the foregoing definitions and hereinafter, the term formyl refers to a radical of formula -CH(=O). When X¹ or X² represents the divalent radical -O-N=CH-, said radical is attached with the carbon atom to the R³, R⁴ bearing cyclic moiety, respectively the R¹, R² bearing phenyl moiety of the compounds of formula (I).

The heterocycles as mentioned in the above definitions and hereinafter, are meant to include all possible isomeric forms thereof, for instance pyrrolyl also includes 2H- pyrrolyl; triazolyl includes 1,2,4-triazolyl and 1,3,4-triazolyl; oxadiazolyl includes 1,2,3-oxadiazolyl, 1,2,4-oxadiazolyl, 1,2,5-oxadiazolyl and 1,3,4-oxadiazolyl; thiadiazolyl includes 1,2,3-thiadiazolyl, 1,2,4-thiadiazolyl, 1,2,5-thiadiazolyl and 1,3,4- thiadiazolyl; pyranyl includes 2H-pyranyl and 4H-pyranyl. Further, the heterocycles as mentioned in the above definitions and hereinafter may be attached to the remainder of the molecule of formula (I) through any ring carbon or heteroatom as appropriate. Thus, for example, when the heterocycle is imidazolyl, it may be a 1 -imidazolyl, 2-imidazolyl, 3-imidazolyl, 4-imidazolyl and 5-imidazolyl; when it is thiazolyl, it may be 2-thiazolyl, 4-thiazolyl and 5-thiazolyl; when it is triazolyl, it may be 1,2,4-triazol-l-yl, l,2,4-triazol-3-yl, l,2,4-triazol-5-yl, 1,3,4-triazol- 1-yl and l,3,4-triazol-2-yl; when it is benzothiazolyl, it may be 2-benzothiazolyl, 4- benzothiazolyl, 5-benzothiazolyl, 6-benzothiazolyl and 7-benzothiazolyl.

The pharmaceutically acceptable addition salts as mentioned hereinabove are meant to comprise the therapeutically active non-toxic acid addition salt forms which the compounds of formula (I) are able to form. The latter can conveniently be obtained by treating the base form with such appropriate acid. Appropriate acids comprise, for example, inorganic acids such as hydrohalic acids, e.g. hydrochloric or hydrobromic acid; sulfuric; nitric; phosphoric and the like acids; or organic acids such as, for example, acetic, propanoic, hydroxyacetic, lactic, pyruvic, oxalic, malonic, succinic (i.e. butane-dioic acid), maleic, fumaric, malic, tartaric, citric, methanesulfonic, ethanesulfonic, benzenesulfonic,^7-toluenesulfonic, cyclamic, salicylic, ;-aminosalicyhc, pamoic and the like acids.

The pharmaceutically acceptable addition salts as mentioned hereinabove are meant to comprise the therapeutically active non-toxic base addition salt forms which the compounds of formula (I) are able to form. Examples of such base addition salt forms are, for example, the sodium, potassium, calcium salts, and also the salts with pharmaceutically acceptable amines such as, for example, ammonia, alkylamines, benzathine, N-methyl-D-glucamine, hydrabamine, amino acids, e.g. arginine, lysine.

Conversely said salt forms can be converted by treatment with an appropriate base or acid into the free acid or base form.

The term addition salt as used hereinabove also comprises the solvates which the compounds of formula (I) as well as the salts thereof, are able to form. Such solvates are for example hydrates, alcoholates and the like. . t

The term stereochemically isomeric forms as used hereinbefore defines the possible different isomeric as well as conformational forms which the compounds of formula (I) may possess. Unless otherwise mentioned or indicated, the chemical designation of compounds denotes the mixture of all possible stereochemically and conformationally isomeric forms, said mixtures containing all diastereomers, enantiomers and/or conformers of the basic molecular structure. All stereochemically isomeric forms of the compounds of formula (I) both in pure form or in admixture with each other are intended to be embraced within the scope of the present invention.

Some of the compounds of formula (I) may also exist in their tautomeric forms. Such forms although not explicitly indicated in the above formula are intended to be included within the scope of the present invention.

The N-oxide forms of the compounds of formula (I) are meant to comprise those compounds of formula (I) wherein one or several nitrogen atoms are oxidized to the so-called N-oxide. A preferred group of compounds consists of those compounds of formula Q) wherein one or more of the following restrictions apply : Z represents NH; Y represents -C_3-9alkyl-, -C_2-9alkenyl-, - .salkyl-oxy-Ci-salkyl-, -C_1-5alkyl-NR¹³-C_1-5alkyl-, -Cj-ealkyl-NH-CO-, -CO-Cι_-7alkyl-, -Cι_-7alkyl-CO- or Ci-ealkyl-CO-Ci-ealkyl; X¹ represents O, -O-C_1-2alkyl-, -O-N=CH-, NR¹¹ or -jSrR^π-Cι_-2alkyl-; in a particular embodiment X¹ represents -NR¹¹-, -O- or -O-CH₂-; X² represents a direct bond, O, -O-C_1-2alkyl-, -O-N=CH-, Cι_-2alkyl, NR¹² or NR¹²-Cι_-2alkyl-; in a particular embodiment X² represents a direct bond, -O-N=CH-, Cι_-2alkyl-, -O-Cι_-2alkyl, -O- or -O-CH₂-; R¹ represents hydrogen, cyano, halo or hydroxy, preferably halo; R² represents hydrogen, cyano, halo, hydroxy, hydroxycarbonyl-,

Het¹⁶-carbonyl-,

C2-₆alkynyl-, Ar⁵ or Het¹; In a further embodiment R² represents hydrogen, cyano, halo, hydroxy, orAr⁵; R³ represents hydrogen;

R⁴ represents hydrogen, hydroxy,

Ar⁴-Cι_-4alkyloxy or R⁴ represents

substituted with one or where possible two or more substituents selected from R¹¹ represents hydrogen, C_1- alkyl- or C alkyl-oxy-carbonyl-; R¹² represents hydrogen,

R¹³ represents

in particular moφholinyl-CMalkyl; Het¹ represents thiazolyl optionally substituted with amino, C_1- alkyl, hydroxy-C_1-4alkyl-, phenyl, phenyl-C_1-4aIkyl-,

mono- or di(Ci-4alkyι)amino- or amino-carbonyl-; Het² represents a heterocycle selected from morpholinyl, piperazinyl, piperidinyl or pyrrolidinyl wherein said Het² is optionally substituted with one or where possible two or more substituents selected from hydroxy, amino or

In a further embodiment Het² represents a heterocycle selected from moφholinyl or piperidinyl optionally substituted with

preferably methyl; Het¹⁴ represents a heterocycle selected from moφholinyl, piperazinyl, piperidinyl or pyrrolidinyl wherein said Het¹⁴ is optionally substituted with one or where possible two or more substituents selected from hydroxy, amino or

Het¹⁶ represents a heterocycle selected from piperidinyl, morpholinyl or pyrrolidinyl; Ar⁴ represents phenyl optionally substituted with cyano, hydroxy- or C_1-4alkyl; Ar⁵ represents phenyl optionally substituted with cyano, hydroxy,

r

A further group of compounds consists of those compounds of formula (I) wherein one or more of the following restrictions apply : Z represents NH;

Y represents -C_3-9alkyl-, -C_1-5alkyl-NR¹³-Cι_-5alkyl-, -Ci-ealkyl-NH-CO- or -CO-NH -Ci-ealkyl- ;

X¹ represents -O- or -NR¹¹-;

X² represents a direct bond, -Cι.2alkyl-, -O-Cι_-2alkyl, -O- or -O-CH₂-; R¹ represents hydrogen or halo;

R² represents hydrogen, cyano, halo, hydroxycarbonyl-, Cι-₄alkyloxycarbonyl-, Het¹ -carbonyl- orAr⁵;

R³ represents hydrogen;

R⁴ represents hydrogen, hydroxy,

or R⁴ represents C_1-4alkyloxy substituted with one or where possible two or more substituents selected from

R¹¹ represents hydrogen;

R¹² represents hydrogen,

R¹³ represents Het¹⁴-Cι- alkyl, in particular

Het² represents a heterocycle selected from moφholinyl, piperazinyl, piperidinyl or pyrrolidinyl wherein said Her² is optionally substituted with one or where possible two or more substituents selected from hydroxy, amino or

In a further embodiment Het² represents a heterocycle selected from moφholinyl or piperidinyl optionally substituted with C_1-4alkyl-, preferably methyl; Het¹⁴ represents moφholinyl; Het¹⁶ represents a heterocycle selected from moφholinyl or pyrroUdinyl; Ar⁴ represents phenyl; Ar⁵ represents phenyl optionally substituted with cyano.

Another group of compounds consists of those compounds of formula (I) wherein one or more of the following restrictions apply : Z represents NH; Y represents -C_3-9alkyl-, -C_2-9alkenyl-, -Ci-salkyl-oxy-Ci-salkyl-,

CO-C_1-7alkyl-, -Cι_-7alkyl-CO- or Cι^alkyl-CO-C_1-6alkyl; X¹ represents O, -O-C_1-2alkyl-, -O-N=CH-, NR¹¹ or -NR^π-Cι_-2alkyl-; in a particular embodiment X¹ represents a direct bond, Cι_-2alkyl-, -O-Cι_-2alkyl,-NR^π-, -O- or -O-CH₂-; X² represents a direct bond, O, -O-C_1-2alkyl-, -O-N=CH-, NR¹⁷-CO-, NR¹⁷-CO-Cι_-2alkyl-, Cι_-2alkyl, Het²⁰-C_1-2alkyl-, NR¹² or NR¹²-Cι_-2alkyl-; in a particular embodiment X² represents a direct bond, Cι_-2alkyl-, -O-Cι_-2alkyl, NR¹⁷-CO-, NR¹⁷-CO-Cι_-2alkyl-, Het ⁰-Cι_-2alkyl-, -O- or -O-CH₂-;

R¹ represents hydrogen, cyano, halo or hydroxy, preferably halo; R² represents hydrogen, cyano, halo, hydroxy, hydroxycarbonyl-,

C_2-6alkynyl-, Ar⁵ or Het¹; in a further embodiment R² represents hydrogen, cyano, halo, hydroxy, or Ar⁵; in a more particular embodiment R² represents hydrogen or halo;

R³ represents hydrogen; R⁴ represents hydrogen, hydroxy,

or R⁴ represents

substituted with one or where possible two or more substituents selected from C_1- alkyloxy- or Het²-;

R¹¹ represents hydrogen,

R¹² represents hydrogen, C_1- alkyl- or Ci₄alkyl-oxy-carbonyl-; R¹³ represents hydrogen or Het¹⁴-Cι- alkyl, in particular moφholinyl-C_Malkyl; R¹⁴ represents hydrogen R¹⁷ represents hydrogen,

in particular R¹⁷ represents hydrogen or C_halky!; Het¹ represents thiazolyl optionally substituted with amino, C^alkyl, hydroxy-C_1-4alkyl-, phenyl,

mono- or

or amino-carbonyl-; Het² represents a heterocycle selected from morpholinyl, piperazinyl, piperidinyl or pyrrolidinyl wherein said Het² is optionally substituted with one or where possible two or more substituents selected from hydroxy, amino or Cι-4alkyl-; In a further embodiment Het² represents a heterocycle selected from moφholinyl or piperidinyl optionally substituted with

Het¹⁶ represents a heterocycle selected from piperidinyl, moφholinyl or pyrrolidinyl; Het²⁰ represents a heterocycle selected from pyrrolidinyl, 2-pyrrolidinyl or piperidinyl; Het²¹ represents a heterocycle selected from morpholinyl, piperazinyl, piperidinyl or pyrrolidinyl wherein said Het² is optionally substituted with one or where possible two or more substituents selected from hydroxy, amino or C_1- alkyl-;

Ar⁴ represents phenyl optionally substituted with cyano, hydroxy-,

or C_1-4alkyl; Ar⁵ represents phenyl optionally substituted with cyano, hydroxy, ^alkyloxy or

Y represents -C_3-9alkyl-, -C_1-5alkyl-NR¹³-Cι_-5alkyl-, -Cι_-5alkyl-NR¹⁴-CO-C_1-5alkyl-, - C_1-6alkyl-NH-CO- or -CO-NH -C_1-6alkyl- ;

X¹ represents a direct bond, -Cι-₂alkyl-, -O-Cι_-2alkyl, -O-, - -CH₂- or -NR¹¹-; X² represents -O-, -O-Ci_-2alkyl, -NR¹²-, NR¹²-Cι_-2alkyl, -NR¹⁷-CO-, NR¹⁷-CO-Cι. ₂alkyl or He -C^alkyl-; R¹ represents hydrogen or halo; R² represents hydrogen, cyano, halo, hydroxycarbonyl-,

Het¹⁶-carbonyl- or Ar⁵; in particular R² represents hydrogen or halo; R³ represents hydrogen;

R⁴ represents hydrogen, hydroxy,

Ar⁴-Cι_-4alkyloxy or R⁴ represents

substituted with one or where possible two or more substituents selected from Cι_4alkyloxy- or Het²-;

R¹¹ represents hydrogen;

R¹² represents hydrogen,

R¹³ represents hydrogen or

in particular hydrogen or

R¹⁴ represents hydrogen; R¹⁷ represents hydrogen;

Het² represents a heterocycle selected from moφholinyl, piperazinyl, piperidinyl or pyrrolidinyl wherein said Het² is optionally substituted with one or where possible two or more substituents selected from hydroxy, amino or

In a further embodiment Het² represents a heterocycle selected from moφholinyl or piperidinyl optionally substituted with preferably methyl; Het¹⁴ represents moφholinyl; Het¹⁶ represents a heterocycle selected from moφholinyl or pyrrolidinyl;

Het²⁰ represents pyrrolidinyl or piperidinyl;

Ar⁴ represents phenyl;

Ar⁵ represents phenyl optionally substituted with cyano.

Other special group of compounds are:

- those compounds of formula (10 wherein a^a²^³^⁴ represents N-CH=CH-CH;

- those compounds of formula (I) wherein a^^-a⁴ represents N-CH=N-CH; - those compounds of formula (T) wherein a¹-a²=a³-a⁴ represents CH-CH=N-CH;

- those compounds of formula (I) wherein -X¹- represents -O-;

- those compounds of formula (I) wherein -X¹- represents -NR¹¹-, in particular -NH-;

- those compounds of formula (I) wherein -X²- represents -NR¹⁷-CO-Cι_-2alkyl-, in particular -NH-CO-Cι_-2alkyl-; - those compounds of formula (I) wherein -X²- represents represents

in particular -NH-C_1-2alkyl-;

- those compounds of formula (I) wherein -Y- represents -Ci-salkyl-NR¹⁴-CO-Ci_ ₅alkyl-, in particular -Ci-salkyl-NH-CO-Ci-salkyl-;

- those compounds of formula (I) wherein R¹ is fluoro, chloro or bromo; - those compounds of formula (I) wherein R² is fluoro, chloro or bromo;

- those compounds of formula (I) wherein R¹ and R² represent halo, in particular those compounds of formula (I) wherein R¹ represents fluoro and R² represents chloro;

- those compounds of formula (I) wherein R² is Het¹, in particular thiazolyl optionally substituted with methyl; - those compounds of formula (I) wherein R² is C₂^alkynyl-, in particular ethylyn;

- those compounds of formula (I) wherein R² is Ar⁵, in particular phenyl optionally substituted with cyano;

- those compounds of formula (I) wherein R³ is cyano;

- those compounds of formula (I) wherein R⁴ represents methoxy and wherein said methoxy is at position 7 of the structure of formula (I).

- those compounds of formula (I) wherein R⁴ represents

substituted with one substituent selected from C_1- alkyloxy- or Het²-, in particular propyloxy substituted with moφholinyl;

- those compounds of formula (T) wherein R¹² is hydrogen or

in particular methyl or wherein R¹² is

in particular t-butyl-oxy-carbonyl- those compounds of formula (I) wherein Het² represent moφholinyl optionally substituted with

preferably moφholinyl attached through the nitrogen atom to the remainder of the compounds of formula (I); those compounds of formula (I) with Het³ represent morpholinyl optionally substituted with

preferably moφholinyl attached through the nitrogen atom to the remainder of the compounds of formula (I); those compounds of formula (I) wherein Het¹² represent morpholinyl optionally substituted with C_1- alkyl, preferably moφholinyl attached through the nitrogen atom to the remainder of the compounds of formula (I).

In a further embodiment of the present invention the R¹ substituent is at position 4', the R² substituent is at position 5% the R³ substituent is at position 2 and the R⁴ substituent at position 6 of the structure of formula (I). A particular group of compounds according to the present invention are those compounds of formula (I) wherein the aniline fragment is substituted with an R² substituent at position 5' and an R¹ substituent at position 4'and wherein said R¹ substituent represents halo, in particular fluoro and wherein said R² substituent is being selected from the group consisting of halo,

Het¹⁶-carbonyl-, hydroxycarbonyl-, cyano, or Ar⁵; in particular said R² being selected from chloro, bromo, methoxycarbonyl, pyrroUdino- carbonyl, moφholino-carbonyl, hydroxycarbonyl, cyano or phenyl.

The compounds of this invention can be prepared by any of several standard synthetic processes commonly used by those skilled in the art of organic chemistry and described for instance in the following references; 'Ηeterocyclic Compounds" - Nol.24 (part4) p 261-304 Fused pyrimidines, Wiley - Interscience ; Chem. Pharm. Bull., Nol 41 (2) 362- 368 (1993); J.Chem.Soc, Perkin Trans. 1, 2001, 130-137.

In brief, for those compounds of formula (I) where -X¹- represents -ΝH- said compounds are generally prepared by reacting the 4-cUoro-6-fluoro-pyridopyrimidines or 4,6-dichloro-pyridopyrimidines of formula (H) with an appropriate aniline (HD using art known reaction conditions, such as for example using a base such as triethylamine, N-e yl-N-(l-memylemyl)-2-propaneamine (DIPEA) and alike or an inorganic base such as Νa₂CO₃, K₂CO₃ and alike in a suitable polar solvent such as propane-2-ol, 1- butanol, acetonitrile and alike at elevated temperatures (60-90°C or reflux temperatures). The thus obtained anilmopyridopyrimidens (TV) are in a further step substituted by a suitable amine of formula (VII) to give the intermediate of formula Vm. This second substitution reaction is performed under known reactions conditions, such as for example, by stirring the reagentia at an elevated temperature (70-100°C) optionally in an appropriate solvent such as propane-2-ol, 1-butanol or DMSO in the presence of abase such as for example triethylamine, N-ethyl-N-(l-methylethyl)-2- propaneamine (DIPEA) and alike. The compounds according to the invention are finally obtained after deprotection and ring closure using art known conditions. Ring closure is typically performed in the presence of a coupling reagent such as for example l,3-ώcyclohexylcarbo<iiimide (DCC), N.N-carbonylmimidazole (GDI), POCl₃, TiC , sulfur chloride fluoride (SO₂ClF) or l-(3-dimethylaminopropyl)-3-ethylcarbodiimide (EDCI) in the presence or absence of hydroxybenzotrialzole (HOBt).

Scheme 0

Pi andP₂ each independently represent optionally protected fiinctional groups, such as for example a primary or secondary amine, hydroxyl, hydroxycarbonyl, or halo (CI, Br or I), which upon reaction produce together with the Yi respectively Y₂ substituents to which they are attached, the divalent Y radical as defined for the compounds of formula (I) hereinbefore. X¹, X², R¹, R², R³ andR⁴ are defined as for the compounds of formula (I) hereinbefore.

As further exemplified in the experimental part of the description, the group of compounds of formula (I) were -X¹- represents -O-, hereinafter referred to as compounds of formula (I'), are generally prepared using the following synthesis scheme. The compounds of this invention may be prepared by coupling the known 4- cHoro-6-cUoropyrimidopyrimidine (II) with suitable substituted anilines (HI), which in their turn can be prepared according to reaction schemes 3-7, furnish the intermediate compounds (TV). Substitution under art known conditions of the 6-chloro group with an appropriate alkoxide, such as for example benzyloxide, methoxide, 2- trimethylsilylethanol, should give upon deprotection, respectively catalytic hydrogenation, TMSC1, Na₂S, TFA, the desired Mitsunobu precursor of formula (VI) (Scheme 1). Next, ring closure under Mitsunobu conditions give the target compounds (I')- Scheme 1

V = hydrogen or a protective group such as for example, methylcarbonyl, t-butyl, methyl, ethyl, benzyl or trialkylsilyl groups; R represents benzyl or memyl; and a'-a -a⁴, Y, X², R¹, R², R³ and R⁴ are defined as for the compounds of formula (I)

Those compounds of formula (I'), where X² represents -O- and a^a^a'-a⁴ represents N-C=N-C are prepared by coupling the known 8-cUoro-2(methyltWo)-pyrimido[5,4- djpyrimidine (XXVII) with 2-aminophenol derivatives of formula (XXNIH) yielding the intermediate compounds of formula (XXLX). Next, after protection of the phenol and oxidation of the methylthio, the pyrimidopyriniidine of formula (V T) is converted into the intermediate of formula (LX) using the appropriate alkoxide. Subsequent deprotection followed by ring closure under Mitsunobu conditions should give the target compounds of formula (I"). Scheme 2

(xxvm)

V = hydrogen or a protective group such as for example, methylcarbonyl, t-butyl, methyl, ethyl, benzyl or trialkylsilyl groups; and Y, X², R¹, R², R³ and R⁴ are defined as for the compounds of formula (I)

Alternatively, those compounds of formula (F), where X represents -O- and a¹-a²=^a³-a⁴ represents C-C=C-N, said compounds are prepared by coupling the known 4-c oro-6-fluoropyridopvrimidines (TT) with 2-aminophenol derivatives of formula (XXVDI) yielding the intermediate compounds of formula (VII). Next, after protection of the phenol, the pyridopyrimidine of formula (VTTT) is converted into the intermediate of formula (LX) using the appropriate alkoxide. Subsequent deprotection followed by ring closure under Mitsunobu conditions should give the target compounds of formula (I"). Scheme 3

Protection Phenol

V = hydrogen or a protective group such as for example, metiiylcarbonyl, t-butyl, methyl, ethyl, benzyl or uialkylsilyl groups; and Y, R¹, R², R³ and R⁴ are defined as for the compounds of formula (I)

For those compounds where X represents -O-, the suitable substituted anilines of formula (UP) are generally prepared from the commercially available nitro-phenols (X) and the , ω-protected halogenated alcohols (XT) under alkaline conditions in a reaction inert solvent, for example, using dimethylacetamide (DMA) in the presence of K₂CO₃. The resulting nitro-phenyl derivative (XH) is subsequently reduced according to standard conditions, for example, using iron/acetic acid, to yield the substituted anilines of formula (HI³) (Scheme 4). Scheme 4

(X) (XI) (XII)

Reduction

(πf) X represents a halogen such as for example, CI, Br and I V represents a protective group such as for example methylcarbonyl

For those compounds where X² represents -NR¹²-or -NR¹²-Cι_-2alkyl-, the suitable substituted anilines of formula (HF) are generally prepared from the commercially available 2-nitro-benzaldehydes (XIH) and the amine substituted alcohols (XIV) by reductive amination under standard conditions, for example using NaBHt and titanium(iv)isopropoxide as reducing agents in ethanol as solvent, yielding in a first step the nitro-ben2ylamines of formula (XV).

Next the primary free alcohol is protected using art known procedures, for example, using an esterification reaction with acetic anhydride in the presence of pyridine. The thus obtained intermediate of formula (XVT) is subsequently reduced according to standard conditions, for example, using iron/acetic acid to yield the substitated anilines of formula (HI^b) (Scheme 5). Scheme 5 Reductive

Amination

(xm) (XIV) (XV)

V represents a protective group such as for example methylcarbonyl m m == 00 o orr 11 a anndd n n == 11 o orr 22 For those compounds where X² represents -O-N=CH-, the suitable substitated anilines of formula (ID*) are generally prepared according to reaction scheme 5. In a first step the known 2-nitro-benzaldehydes (XTTT) are converted into the corresponding oxime (XVII) using, for example, the art known condensation reaction with hydroxylarnine.

Next said oxime of formula XVH is allowed to react for example, with an halogenated alkylacetate under alkaline conditions, for example using K2CO₃ in DMSO or with a stronger silyl protecting group like TBDMS or TBDPS, and NaH in THF for the reaction conditions, followed by reducing the nitro group, for example, with hon/ acetic acid, to provide the suitable substituted aniline of formula (HI⁰). Scheme 6

(xm) (xvn) ₀ (xvπn

X represents a halogen such as for example CI, Br or I

For those compounds where X² represents a direct bond and Y represents Ci-βalkyl- NH-CO-, the suitable substitated anilines of formula (III^d) are generally prepared according to reaction scheme 7.

In a first step the known 2-nitro-benzoic acids (XX) are amidated to the intermediates of formula (XXH) under art known conditions, for example, using a hydroxylated amine of formula (XXI) that is added dropwise to a mixture of (XX) in CH₂C1₂ in the presence of 1,1 'carbonylbis-lH-imidazole.

Next the primary free alcohol is protected using art known procedures, for example, using an esterification reaction with acetic anhydride in the presence of pyridine. The thus obtained intermediate of formula (XXIII) is subsequently reduced according to standard conditions, for example, using iron/acetic acid to yield the substituted anilines of formula (HI^d).

Reduction

V represents a protective group such as for example methylcarbonyl

For those compounds where X represents a direct bond the suitable substitated anilines of formula ( f) are generally prepared according to reaction scheme 7. In a first step the known 2-nitro-benzaldehydes ( TT) are alkenated to the intermediates of formula (XXV) under art known conditions, for example, using the Wittig Reaction with the appropriate phosphonium salt of formula (XXTV). Following esterification of the free carboxylic acid under standard conditions for example, using ethanol under acidic conditions, the intermediate of formula (XXVT) are reduced to yield the desired substituted anilines of formula (HI⁶). Scheme 8

Esterification

Reduction

(XXVT) Yj represents a C_1-7alkyl

As further exemplified in the experimental part of the description, the group of compounds of formula (!) were -X¹- represents -NR¹¹- and and a'-aW-a⁴ represents N- CH=N-ChT, hereinafter referred to as compounds of formula (F"), are generally prepared ixsing the following synthesis scheme (Scheme 9). Said compounds may be prepared by coupling the known 8-c oro-2(me1hyltMo)-pvrimido[5,4-d]pyrimidine with 2-aminophenol derivatives of formula (XXVID), yielding the intermediate compounds of formula (XXLX).

Next, the pyrintido[5,4-d]pyrimidine of formula (XXIX) is aminated using an aminated alcohol (XXX) under art known conditions, followed by ring closure under Mitsunobu conditions to give the target compounds of formula (I"'). Scheme 9

V = Protective group + R¹ N-Y— OH (X X)

Alternatively, for those compounds of formula (I) where -X¹- represents -NR¹¹- and and a¹-a²=a³-a⁴ represents N-CH=CH-CH, the compounds are prepared by coupling the known 4,6-dichloro- (XXVTF) with 2-aminophenol derivatives of formula XVDI), yielding the intermediate compounds of formula (XXLX').

Next, the pyrido[3,2-d]pyrimidine of formula (XXLX') is aminated using an aminated alcohol (X XX) under art known conditions, followed by ring closure under Mitsunobo conditions to give the target compounds of formula (I"") (Scheme 10). Scheme 10

+ R¹¹N-Y— OH (XXX)

Where necessary or desired, any one or more of the following further steps in any order may be performed :

(i) removing any remaining protecting group(s);

(ii) converting a compound of formula (I) or a protected form thereof into a further compound of formula (I) or a protected form thereof; (iii) converting a compound of formula (I) or a protected form thereof into a N-oxide, a salt, a quaternary amine or a solvate of a compound of formula (I) or a protected form thereof; (iv) converting a N-oxide, a salt, a quaternary amine or a solvate of a compound of formula (I) or a protected form thereof into a compound of formula (I) or a protected form thereof; (v) converting a N-oxide, a salt, a quaternary amine or a solvate of a compound of formula (I) or a protected form thereof into another N-oxide, a pharmaceutically acceptable addition salt a quaternary amine or a solvate of a compound of formula (I) or a protected form thereof; (vi) where the compound of formula (I) is obtained as a mixture of (R) and (S) enantiomers resolving the mixture to obtain the desired enantiomer.

Compounds of formula (I), N-oxides, addition salts, quaternary amines and stereochemical isomeric forms thereof can be converted into further compounds according to the invention using procedures known in the art. It will be appreciated by those skilled in the art that in the processes described above the functional groups of intermediate compounds may need to be blocked by protecting groups.

Functional groups, which it is desirable to protect, include hydroxy, amino and carboxylic acid. Suitable protecting groups for hydroxy include trialkylsilyl groups (e.g. tert-butyld αethylsilyl, tert-butyldiphenylsilyl or trimethylsilyl), benzyl and tetrahydropyranyl. Suitable protecting groups for amino include tert-butyloxycarbonyl or benzyloxycarbonyl. Suitable protecting groups for carboxylic acid include

or benzyl esters.

The protection and deprotection of functional groups may take place before or after a reaction step.

Additionally, the JST-atoms in compounds of formula (I) can be methylated by art- known methods using CH3-I in a suitable solvent such as, for example 2-propanone, tetrahydrofuran or dimethylformamide.

The compounds of formula (I) can also be converted into each other following art- known procedures of functional group transformation of which some examples are mentioned hereinafter.

The compounds of formula (I) may also be converted to the corresponding N-oxide forms following art-known procedures for converting a trivalent nitrogen into its N-oxide form. Said N-oxidation reaction may generally be carried out by reacting the starting material of formula (T) with 3-phenyl-2-(phenylsulfonyl)oxaziridine or with an appropriate organic or inorganic peroxide. Appropriate inorganic peroxides comprise, for example, hydrogen peroxide, alkali metal or earth alkaline metal peroxides, e.g. sodium peroxide, potassium peroxide; appropriate organic peroxides may comprise peroxy acids such as, for example, benzenecarboperoxoic acid or halo substituted benzenecarboperoxoic acid, e.g. 3-chlorobenzenecarboperoxoic acid, peroxoalkanoic acids, e.g. peroxoacetic acid, alkylhydroperoxides, e.g. t-butyl hydroperoxide. Suitable solvents are, for example, water, lower alkanols, e.g. ethanol and the like, hydrocarbons, e.g. toluene, ketones, e.g. 2-butanone, halogenated hydrocarbons, e.g. dichloromethane, and mixtures of such solvents. Pure stereochemically isomeric forms of the compounds of formula (I) maybe obtained by the application of art-known procedures. Diastereomers may be separated by physical methods such as selective crystallization and chromatographic techniques, e.g. counter-current distribution, liquid chromatography and the like.

Some of the compounds of formula (I) and some of the intermediates in the present invention may contain an asymmetric carbon atom. Pure stereochemically isomeric forms of said compounds and said intermediates can be obtained by the application of art-known procedures. For example, diastereoisomers can be separated by physical methods such as selective crystallization or chromatographic techniques, e.g. counter current distribution, liquid chromatography and the like methods. Enantiomers can be obtained from racemic mixtures by first converting said racemic mixtures with suitable resolving agents such as, for example, chiral acids, to mixtures of diastereomeric salts or compounds; then physically separating said mixtures of diastereomeric salts or compounds by, for example, selective crystallization or chromatographic techniques, e.g. liquid chromatography and the like methods; and finally converting said separated diastereomeric salts or compounds into the corresponding enantiomers. Pure stereochemically isomeric forms may also be obtained from the pure stereochemically isomeric forms of the appropriate intermediates and starting materials, provided that the intervening reactions occur stereospecifically.

An alternative manner of separating the enantiomeric forms of the compounds of formula (I) and intermediates involves liquid chromatography, in particular liquid chromatography using a chiral stationary phase.

Some of the intermediates and starting materials as used in the reaction procedures mentioned hereinabove are known compounds and may be commercially available or may be prepared according to art-known procedures. However, in the synthesis of the compounds of formula (I), the present invention further provides the intermediates of formula (HI)

(HI) the pharmaceutically acceptable addition salts and the stereochemically isomeric forms thereof, wherein N represents hydrogen or a protective group preferably selected from the group consisting of methylcarbonyl, t-butyl, methyl, ethyl, benzyl or trialkylsilyl; Y represents -C_3-9alkyl-, -C3- alkenyl-, -Cι-5alkyl-oxy-C_1-5alkyl-, -Cι_-5alkyl-r ¹³-Cι_-5alkyl-,

-C_1-5alkyl-CO-ΝR¹⁵-Cι_-5alkyl-, -Ci-ealkyl-CO-NH-, -Ci-ealkyl-NH-CO-, -Cι_-7alkyl-CO-, Ci-ealkyl-CO-Ci.ealkyl; X² represents a direct bond, O, -O-Cι_₂alkyl-, CO, -CO- C_1-2alkyl-, NR¹², -NR¹ -Ci.₂alkyl-, -CH₂-, -O-N=CH- or C_1-2alkyl; R¹ represents hydrogen, cyano, halo, hydroxy, formyl, Ci-βalkoxy-, C^alkyl-, Ci-βalkoxy- substituted with halo, substituted with one or where possible two or more substitaents selected from hydroxy or halo; and R² represents hydrogen, cyano, halo, hydroxy, hydroxycarbonyl-, Het¹⁶-carbonyl-,

aminocarbonyl-, mono-or

Het¹, formyl,

C_2-6alkynyl-, C₃-₆cycloalkyl-, C₃-₆cycloalkyloxy-, Ci-βalkoxy-, Ar⁵, Ar¹-oxy-, dihydroxyborane , Cι_₆alkoxy- substituted with halo, substituted with one or where possible two or more substitaents selected from halo, hydroxy or NR⁵R⁶,

wherein said

is optionally substitated with one or where possible two or more substitaents selected from hydroxy or

R⁵ andR⁶ are each independently selected from hydrogen or

R¹² represents hydrogen,

C₂-4alkenylcarbonyl- optionally substitated with Het¹⁹-Cι_-4alkylaminocarbonyl-,

or phenyl optionally substitated with one or where possible two or more substituents selected from hydrogen, hydroxy, amino or

R¹³ represents hydrogen, Chalky!, Het¹³,

or phenyl optionally substituted with one or where possible two or more substituents selected from hydrogen, hydroxy, amino or

R¹⁴ andR¹⁵ are each independently selected from hydrogen,

or Ci-₄alkyloxyC_1-4alkyl-; Het¹ represents a heterocycle selected from piperidinyl, moφholinyl, piperazinyl, furanyl, pyrazolyl, dioxolanyl, thiazolyl, oxazolyl, imidazolyl, isoxazolyl, oxadiazolyl, pyridinyl or pyrrolidinyl wherein said Het¹ is optionally substitated amino,

phenyl,

or amino-carbonyl-;

Het¹³ represent a heterocycle selected from pyrrolidinyl or piperidinyl wherein said heterocycle is optionally substitated with one or where possible two or more substitaents selected from C_1- alkyl, C3-6cycloalkyl,

Het¹⁴ represent a heterocycle selected from morpholinyl, pyrrolidinyl, piperazinyl or piperidinyl wherein said heterocycle is optionally substitated with one or where possible two or more substituents selected from C_1-4alkyl, C_3-6cycloalkyl,

Het¹⁵ represent a heterocycle selected from morpholinyl, pyrrolidinyl, piperazinyl or piperidinyl wherein said heterocycle is optionally substitated with one or where possible two or more substituents selected from

C_3-6Cycloalkyl,

Het¹⁶ represent a heterocycle selected from moφholinyl, pyrrolidinyl, piperazinyl, 1,3,2-dioxaborolane or piperidinyl wherein said heterocycle is optionally substitated with one or more substituents selected from

and

Het¹⁷ represent a heterocycle selected from pyrrolidinyl or piperidinyl wherein said heterocycle is optionally substitated with one or where possible two or more substitaents selected from

C₃-₆Cycloalkyl,

Ci.

or polyhydroxy-C₁. aIkyl-;

Het¹⁸ and Het¹⁹ each independently represent a heterocycle selected from moφholinyl, pyrrolidinyl, piperazinyl or piperidinyl wherein said Het¹⁸ and Het¹⁹ are optionally substituted with one or wiiere possible two or more substituents selected from Qj-ecycloalkyl,

or

Ar¹, Ar², Ar³, Ar⁴ and Ar⁵ each independently represent phenyl optionally substitated with cyano,

aminosulfonylamino-, hydroxy-C_1- alkyl, aminosulfonyl-, hydroxy-,

In particular the intermediates of formula (ID) wherein one or more of the following restrictions apply;

I) Y represents -C_3- alkyl-, -Ci-salkyl-oxy-Ci-salkyl-,

-Cwalkyl-NH-CO-; h) X² represents a direct bond, O, -O-C_1-2alkyl-, NR¹², -NR¹²-C_1-2alkyl-, -CH₂-, -O-N=CH- or Cι_-2alkyl; iii) R¹ represents hydrogen, cyano, halo or hydroxy, preferably halo; iv) R² represents hydrogen, cyano, halo, hydroxy, hydroxycarbonyl-,

Het¹⁶-carbonyl-,

C₂-ealkynyl-, Ar⁵ or Het¹; In a further embodiment R² represents hydrogen, cyano, halo, hydroxy, C2-6alkynyl- or Het¹; in particular R² represents hydrogen, cyano, halo, hydroxy, or Ar⁵; v) R¹² represents hydrogen,

or C_1- alkyloxycarbonyl; vi) R¹³ represents Het¹⁴-C_1-4alkyl, in particular

vii) Het¹ represents thiazolyl optionally substitated with amino,

hydroxy-Cι-₄alkyl-, phenyl, phenyl-Cι_₄alkyl-,

mono- or di(C_1- alkyι)amino- or amino-carbonyl-; viii) Het¹⁶ represents a heterocycle selected from piperidinyl or pyrrolidinyl.

It is also an object of the present invention to provide the use of an intermediate of formula (ID) in the synthesis of a macrocyclic kinase inhibitor such as for example compound of formula (I).

The compounds of formula (I) and the intermediates of formula (XXXI) of the present invention are useful because they possess pharmacological properties. They can therefore be used as medicines.

Accordingly, in a further aspect this invention concerns the intermediates of formula

the N-oxide forms, the pharmaceutically acceptable addition salts and the stereochemically isomeric forms thereof, wherein a¹-a²=a³-a⁴ represents a divalent radical selected from N-CH=CH-CH or N-CH=N-CH; Y represents -C_3-9alkyl-, -C_1-5alkyl-NR¹³-Cι_-5alkyl-, -C_1-6alkyl-NH-CO- or -CO-NH -Ci-ealkyl- ; R¹ represents hydrogen or halo; R² represents hydrogen, cyano, halo, hydroxycarbonyl-, Ci^alkyloxycarbonyl-, Het¹⁶-carbonyl- or Ar⁵; R⁴ represents hydroxy,

or R⁴ represents

substituted with one or where possible two or more substituents selected from C_1-4alkyloxy- or Het²-;

R¹¹ represents hydrogen; R¹³ represents

in particular

In a further embodiment Het² represents a heterocycle selected from moφholinyl or piperidinyl optionally substituted -with

preferably methyl;

Het¹⁴ represents moφholinyl;

Het¹⁶ represents a heterocycle selected from moφholinyl or pyrrolidinyl;

Ar⁴ represents phenyl;

Ar⁵ represents phenyl optionally substituted with cyano; as well as the use of an intermediate of formula (XXXI) in the synthesis of a macrocyclic kinase inhibitor such as for example the compounds of formula (I).

As described in the experimental part hereinafter, the growth inhibitory effect and anti- tumour activity of the present compounds and some of the intermediates has been demonstrated in vitro, in enzymatic assays on the receptor tyrosine kinase EGFR. In an alternative assay, the growth inhibitory effect of the compounds was tested on the ovarian carcinoma cell line SKOV3 using art known cytotoxicity assays such as LIVE/DEAD (Molecular Probes) or MTT.

Accordingly, the present invention provides the compounds of formula (I) and the intermediates of formula (XXXI) and their pharmaceutically acceptable N-oxides, addition salts, quaternary amines and stereochemically isomeric forms for use in therapy. More particular in the treatment or prevention of cell proliferation mediated diseases. The compounds of formula (I), the intermediates of formula (XXXI) and their pharmaceutically acceptable N-oxides, addition salts, quaternary amines and the stereochemically isomeric forms may hereinafter be referred to as compounds according to the invention.

Disorders for which the compounds according to the invention are particularly useful are atherosclerosis, restenosis, cancer and diabetic complications e.g. retinopathy. In view of the utility of the compounds according to the invention, there is provided a method of treating a cell proliferative disorder such as atherosclerosis, restenosis and cancer, the method comprising administering to an animal in need of such treatment, for example, a mammal including humans, suffering from a cell proliferative disorder, a therapeutically effective amount of a compound according to the present invention.

Said method comprising the systemic or topical administration of an effective amount of a compound according to the invention, to animals, including humans. One skilled in the art will recognize that a therapeutically effective amount of the EGFR inhibitors of the present invention is the amount sufficient to induce the growth inhibitory effect and that this amount varies inter alia, depending on the size, the type of the neoplasia, the concentration of the compound in the therapeutic formulation, and the condition of the patient. Generally, an amount of EGFR inhibitor to be administered as a therapeutic agent for treating cell proliferative disorder such as atherosclerosis, restenosis and cancer, will be determined on a case by case by an attending physician.

Generally, a suitable dose is one that results in a concentration of the EGFR inhibitor at the treatment site in the range of 0.5 nM to 200 μM, and more usually 5 nM to 10 μM. To obtain these treatment concentrations, a patient in need of treatment likely will be administered between 0.01 mg/kg to 30O mg/kg body weight, in particular from 10 mg/kg to 100 mg/kg body weight. As noted above, the above amounts may vary on a case-by-case basis. In these methods of treatment the compounds according to the invention are preferably formulated prior to admission. As described herein below, suitable pharmaceutical formulations are prepared by known procedures using well known and readily available ingredients .

Due to their high degree of selectivity as EGFR inhibitors, the compounds of formula (I) and the intermediates of formula (XXXI) as defined above, are also useful to mark or identify the kinase domain wilhin the receptor tyrosine kinase receptors. To this puφose, the compounds of the present invention can be labelled, in particular by replacing, partially or completely, one or more atoms in the molecule by their radioactive isotopes. Examples of interesting labelled compounds are those compounds having at least one halo which is a radioactive isotope of iodine, bromine or fluorine; or those compounds having at least one ^x lC-atom or tritium atom. One particular group consists of those compounds of formula (I) and intermediates of formula (XXXI) wherein R is a radioactive halogen atom. In principle, any compound according to the invention containing a halogen atom is prone for radiolabelling by replacing the halogen atom by a suitable isotope. Suitable halogen radioisotopes to this pmpose are radioactive iodides, e.g. ^{1 2}1, ¹²³1, ¹²⁵1, ¹³¹I; radioactive bromides, e.g. ⁷⁵Br, ⁷⁶Br, ⁷⁷Br and ⁸²Br, and radioactive fluorides, e.g. ¹⁸F. The introduction of a radioactive halogen atom can be performed by a suitable exchange reaction or by using any one of the procedures as described hereinabove to prepare halogen derivatives of formula (I). Another interesting form of radiolabelling is by substituting a carbon atom by a "C-atom or the substitution of a hydrogen atom ^"by a tritium atom. Hence, said radiolabelled compounds according to the invention can be used in a process of specifically marking receptor sites in biological material. Said process comprises the steps of (a) radiolabelling a compound according to the invention, (b) administering this radiolabelled compound to biological material and subsequently (c) detecting the emissions from the radiolabelled compound. The term biological material is meant to comprise every kind of material which has a biological origin. More in particular this term refers to tissue samples, plasma or body fluids but also to animals, specially warm-blooded animals, or parts of animals such as organs. When used in in vivo assays, the radiolabelled compounds are adrninistered in an appropriate composition to an animal and the location of said radiolabelled compounds is detected using imaging techniques, such as, for instance, Single Photon Emission Computerized Tomography (SPECT) or Positron Emission Tomography (PET) and the like. In this manner the distribution of the particular receptor sites throughout the body can be detected and organs containing said receptor sites can be visualized by the imaging techniques mentioned hereinabove. This process of imaging an organ by aa ninistering a radiolabelled compound of forrαula (T) and detecting the emissions from the radioactive compound also constitutes apart of the present invention.

In yet a further aspect, the present invention provides the use of the compounds according to the invention in the manufacture of a medicament for treating any of the aforementioned cell proliferative disorders or indications.

The amount of a compound according to the present invention, also referred to here as the active ingredient, which is required to achieve a therapeutical effect will be, of course, vary with the particular compound, the route of administration, the age and condition of the recipient, and the particular disorder or disease being treated A suitable daily dose would be from 0.01 mg/kg to 300 mg/kg body weight, in particular from 10 mg kg to 100 mg/kg body weight. A method of treatment may also include administering the active ingredient on a regimen of between one and four intakes pea- day.

While it is possible for the active ingredient to be administered alone, it is preferable to present it as a pharmaceutical composition. Accordingly, the present invention further provides a pharmaceutical composition comprising a compound according to the present invention, together with a pharmaceutically acceptable carrier or diluent. The carrier or diluent must be "acceptable" in the sense of being compatible with the other ingredients of the composition and not deleterious to the recipients thereof.

The pharmaceutical compositions of this invention may be prepared by any methods well known in the art of pharmacy, for example, using methods such as those described in Gennaro et al. Remington's Pharmaceutical Sciences (18^th ed., Mack Publishing Company, 1990, see especially Part 8 : Pharmaceutical preparations and their Manufacture). A therapeutically effective amount of the particular compound, in base form or addition salt form, as the active ingredient is combined in intimate admixture with a pharmaceutically acceptable carrier, which may take a wide variety of forms depending on the form of preparation desired for administration. These pharmaceutical compositions are desirably in unitary dosage form suitable, preferably, for systemic administration such as oral, percutaneous or parenteral administration; or topical administration such as via inhalation, a nose spray, eye drops or via a cream, gel, shampoo or the like. For example, in preparing the compositions in oral dosage form, any of the usual pharmaceutical media may be employed, such as, for example, water, glycols, oils, alcohols and the like in the case of oral liquid preparations such as suspensions, syrups, elixirs and solutions: or solid carriers such as starches, sugars, kaolin, lubricants, binders, disintegrating agents and the like in the case of powders, pills, capsules and tablets. Because of their ease in adrriiriistration, tablets and capsules represent the most advantageous oral dosage unit form, in which case solid pharmaceutical carriers are obviously employed. For parenteral compositions, the carrier will usually comprise sterile water, at least in large part, though other ingredients, for example, to aid solubility, may be included. Injectable solutions, for example, may be prepared in which the carrier comprises saline solution, glucose solution or a mixture of saline and glucose solution. Injectable suspensions may also be prepared in which case appropriate liquid carriers, suspending agents and the like may be employed. In the compositions suitable for percutaneous acirninistratioix, the carrier optionally comprises a penetration enhancing agent and/or a suitable wettable agent, optionally combined with suitable additives of any nature in minor proportions, which additives do not cause any significant deleterious effects on the skin. Said additives may facilitate the administration to the skin and/or may be helpful for preparing the desired compositions. These compositions may be administered in various ways, e.g., as a transdermal patch, as a spot-on or as an ointment.

It is especially advantageous to formulate the aforementioned pharmaceutical compositions in dosage unit form for ease of administration and uniformity of dosage. Dosage unit form as used in the specification and claims herein refers to physically discrete units suitable as unitary dosages, each unit containing a predetermined quantity of active ingredient calculated to produce the desired therapeutic effect in association with the required pharmaceutical carrier. Examples of such dosage unit forms are tablets (including scored or coated tablets), capsules, pills, powder packets, wafers, injectable solutions or suspensions, teaspoonfuls, tablespoonfuls and the like, and segregated multiples thereof.

Experimental part

Hereinafter, the term 'ADDP' means l,r-(azodicarbonyl)bis- piperidine, 'DMF' means NN-dimethylformamide, 'THF' means tetrahydrofuran, "DMSO" means dimethyl sulfoxide

A. Preparation of the intermediates ^• *

Example Al a) Preparation of phenol, 4-cUoro-2-[(6-cUoropyτido[3,2-^pyrimidin-4- yl)amino]- (intermediate 1) A mixture of 4,6-dichloro- pyrido[3,2-d]pyrimidirιe (0.00255 mol) and 4-chloro-2- aminophenol (0.00446 mol) in isopropanol (30 ml) was stirred at 50°C for 2h30, then brought to room temperature and evaporated to dryness. The residue was taken up in ether, filtered and dried, yielding lg (100%) of intermediate 1. b) Preparation of phenol, 4-c oro-2-[[6-[(6-hyότoxyhexyl)amino]pyrido[3,2- ft pyrinιidm-4-yl]amino]- (intermediate 2) A mixture of intermediate 1 (0.00255 mol) and 6-aιmno-l-hexanol (0.0255 mol) was stirred at 100°C for 3 hours, then brought to room temperature. The residue was purified by chromatography over silica gel (eluent: DCM eOH/ΝBUOH 97/3/0.1; 70- 200μm), yielding 0.71g (72%) of intermediate 2, melting point 260°C. Example A2 Preparation of phenol, 4-chloro-2-[[6-[(4-hydroxybutyl)amino]pyrido[3,2- ^pyrimidm-4-yl]amino]- (intermediate 3)

A mixture of intermediate 1 (0.0013 mol) and 4-amirιo- 1-butanol (0.026 mol) was stirred at 100°C for 4 hours, then brought to room temperature and hydrolyzed a saturated solution of sodium chloride. The mixture was extracted by DCM, decanted, dried over MgSO₄, filtered, and the solvent was evaporated till dryness. The residue (0.5g) was purified by column chromatography over silica gel (eluent:DCM/MeOH/NH₄OH 95/5/0.1; 70-200μm)„ The residue (81mg, 17%) was crystallized from acetonitrile and diethyl ether. The precipitate was filtered off and dried, yielding 69mg (15%) of intermediate 3 , melting point 227°C.

Example A3 Preparation of phenol, 4-cUoro-2-[[6-[(5-hyά oxypen1yl)amino]pyrido[3,2-

(intermediate 4)

A mixture of intermediate 1 (0.0013 mol) and 5-amino-l-ρentanol (0.0195 mol) was stirred at 100°C for 4 hours, then brought to room temperature and hydrolyzed a saturated of sodium chloride. The mixture was extracted by DCM, decanted and dried over MgSO₄, filtered, and the solvent was evaporated till dryness.The residue (0.45g) was purified by column chromatography over silica gel (eluent: DCM/MeOH/NHLtOH 95/5/0.1; 70-200μm). The residue (66mg, 14%) was crystallized from acetonitrile and diethyl ether. The precipitate was filtered off and dried, yielding 59mg (12%) of intermediate 4, melting point 240°C.

Example A4 a) Preparation of phenol, 4-cUcffo-2-[[6-(memylthio)pyrimido[5,4-^pyrimidin-4- yl]amino]- (intermediate 5)

A mixture of 8-chloro-2-(methylthio)- pyrimido[5, -d]pyrimidine (0.0047 mol) and 2- amino-4-chlorophenol (0.0094 mol) in dioxane (5 nol) was stirred at 80°C for 1 hour, then cooled to room temperature, the precipitate was filtered off, washed with water and then with diethyl ether and dried in vacuo, yielding 1.2g (80%) of intermediate 5. b) Preparation of phenol, 4-c oro-2-[[6-[(6-hydroxyhexyl)armrιo]pyrimido[5,4- ^pyrinύ(lm-4-yl]amino]- (intermediate 6)

A mixture of intermediate 1 (0.00172 mol) in 6-amino-l-hexanol (0.0022 mol) was melt at 100°C after 8 hours. The residue was purified by column chromatography over silica gel (eluent: CHaCyCHsOH NHtOH 97/3/0.1; 35-70μm) yielding 0.170g of solid Ether was added The solid was filtered off and dried in vacuo, yielding 135mg (20%) of intermediate (6).

Example A5 a) Preparation of pyrido[3,2-d]pyrimidirιe, 4,6-dichloro- (intermediate 7)

DMF (3 drops) was added to a mixture of 6-cUoro-pyrido[3,2-d]pyrintidin-4(lH)-one [171178-33-9] (0.00275 mol) and thionyl chloride (0.179 mol). The reaction niixture was stirred and refluxed (at 80°C) for 90 minutes. The solvent was evaporated Some dichloromethane was added and the solvent was evaporated The residue was dissolved in dichloromethane. The organic solution was washed with a saturated aqueous K₂CO₃ solution, then dried (MgSO₄), filtered and the solvent was evaporated, yielding 0.49g (89%) of intermediate (7). (HPLC: 85% P). b) Preparation of 4-[2-(6-C oro-pyrido[3,2-d]pyrintidm-4-ylamino)-phenoxy]- butyric acid ethyl ester (intermediate 8)

Intermediate (7) (0.00245 mol) was dissolved in 2-propanol (20 ml) (not very soluble). 4-(2-Aminophenoxy)butanoic acid ethyl ester (0.00416 mol) was added, followed by addition of NN-diethylethanamine (0.00490 mol). The reaction mixture was stirred and refluxed overnight. Then, the reaction mixture was cooled to room temperature and the solvent was evaporated The residue was taken up into diethyl ether. The precipitate was filtered off and dried (pump), yielding 1.48 g of fraction (1) (greenish solid, 92% P by HPLC-MS; presence of some starting material B). This fraction (1) was purified as described below.

The reaction was repeated.

Intermediate (7) (0.0055 mol) was dissolved in 2-propanol (40 ml) (not very soluble). 4-(2-Aminophenoxy)butanoic acid, ethyl ester (0.00935 mol) was added, followed by addition of NN-diethylethanamine (0.0110 mol). The reaction mixture was stirred and refluxed overnight. Then, the reaction mixture was cooled to room temperature and the solvent was evaporated. The residue was combined with fraction (1) and subjected to flash column chromatography over silica gel (eluent: n-hexane/EtOAc 3/1). The product fractions were collected and the solvent was evaporated, yielding 3.04g of intermediate (8)(greenish solid in quantitative yield, used in next reaction step without further purification). c) Preparation of 4-{2-[6-(3-tert-Butoxycarbonylarrnno-propylamino)-pyrido[3,2- d]pyrimidm-4-ylamino]-phenoxy} -butyric acid ethyl ester (intermediate 9)

Intermediate (8) (0.00026 mol) and (3-aminopropyl)carbamic acid 1,1-dimethylethyl ester (0.00288 mol) were mixed for 3 hours at 100°C in a closed reactor, yielding fraction (1) (57% P by HPLC + 35% of the amide).

This fraction (1) was purified as described below.

The reaction was repeated. Intermediate (8) (0.00026 mol) and (3-arninopropyl)carbamic acid 1,1-dimethylethyl ester (0.00288 mol) were mixed for 2.5 hours at 100°C in an open reaction flask (not in a closed reactor as described above). The mixture was combined with fraction (1). Purified by flash column chromatography over silica gel (eluent : n-hexane/EtOAc 3/1). The product fractions were collected and the solvent was evaporated, yielding intermediate (9) (HPLC: 92% P). d) Preparation of 4-{2-[6-(3-Ammo-propylammo)-pyrido[3,2-d]pyrimidin-4- ylaminoj-phenoxy} -butyric acid ethyl ester (intermediate 1O)

Intermediate (9) ( (0.00019 mol) was dissolved in dichloromethane (4.00 ml). Trifluoroacetic acid (0.05192 mol) was added and the reaction mixture was stirred for 2 hours at room temperature. The solvent and remaining acid were evaporated in the rotary evaporator. The resultant residue (oil) was dried (high-vacuum pump), yielding intermediate (10) (HPLC: 93% P; quantitative yield; used in next reaction step, without further purification). e) Preparation of 4-{2-[6-(3-Ammo-propylammo)-pyrido[3₃2-d]pyrmιidin-4- ylaminoj-phenoxy} -butyric acid (intermediate 11) Intermediate (10) (0.00019 mol; 1 equiv) was dissolved in tetrahydofuran (8.00 ml). Water (1.00 ml)was added. Lithium hydroxide monohydrate (0.0019 mol) was added as a solid. More Lithium hydroxide monohydrate was added until a basic pH was reached (until then it was acidic because of CF₃COOH remainders). The reaction mixture was stirred for 2 days at 65°C. The solvent was evaporated in the rotary evaporator, yielding intermediate (11).(HPLC: 78% P; quantitative yield; used in next reaction step, without further purification). Example A6 a) Preparation of 4-cUoro-6-fluoro-pyrido[3,4-d]pyrintidine, (intermediate 12)

DMF (5 drops) was added to a rnixtare of 6-Fluoro-3H-pyrido[3,4-d]pyrimidin-4-one (0.00605 mol) and thionyl chloride (0.39 mol). The reaction rnixtare was stirred and refluxed (at 80°C) for 7 hours. The solvent was evaporated, yielding 1.254 g of intermediate (12) (impure quantitative yield; used in next reaction step, without further purification). b) Preparation of 4-[2-(6-Fluoro-pyrido[3,4-d]pyrimidm-4-ylamino)-phenoxy]- butyric acid ethyl ester (intermediate 13)

Intermediate (12) (0.00605 mol) was dissolved in 2-propanol (40 ml). 4-(2- aminophenoxy)-butanoic acid, ethyl ester [112290-16-1] .hydrochloride (0.01028 mol) was added, followed by addition of N,N-die ylethanamine (0.01210 mol). The reaction mixture was stirred and refluxed overnight. Then, the reaction mixture was cooled to room temperature and the solvent was evaporated. The residue was purified by flash column chromatography over silica gel (eluent : n-hexane/EtOAc 3/1). The product fractions were collected and the solvent was evaporated, yielding 0.922 g of intermediate (13) (41% yield over two steps; yellowish solid; 97% P by HPLC). c) Preparation of 4-{2-[6-(3-tert-Butoxycarbonylarmrιo-propylarmno)-pyrido[3,4- d]pyrimidm-4-ylamino]-phenoxy} -butyric acid ethyl ester (intermediate 14)

Intermediate (13) (0.00027 mol) was dissolved in DMSO (q.s.), in a reactor. (3- Aminopropyl)carbamic acid 1,1-dimethylethyl ester [75178-96-0](0.07 ml) andN- e1hyl-N-(l-memyle l)-2-proρanamine [7087-68-5] (0.10 ml) were added. The reactor was closed and the mixture was heated for 7 days at 80°C. The reaction mixture was poured out into water and the product was extracted three times with dichloromethane. The combined organic layers were dried (MgSO ), filtered and the solvent was evaporated, yielding fraction 1 of intermediate (14).

Two other fractions of Intermediate 14 were prepared as follows :

Intermediate (13) (0.00027 mol) and (3-aminopropyl)carbamic acid 1,1-dimethylethyl ester [75178-96-0] (0.00299 mol) were mixed in a (closed) reactor and heated at 100°C for 3 hours, yielding fraction 2 of intermediate (14). ^"tntermediate (13) (0.00008 mol) and (3-aminopropyl)carbamic acid 1,1-dimethylethyl ester [75178-96-0] (0.0009 mol) were mixed in an open reaction flask and heated at 80°C for 3 days, yielding fraction 3 of intermediate (14)

Fraction 1 , 2 and 3 of intermediate 14 were combined and purified by flash column chromatography over silica gel.

Intermediate 14 was also prepared as follows : Intermediate (13) (0.00027 mol) was dissolved in DMF (3 ml). (3- Aminopropyl)carbamic acid 1,1-dimethylethyl ester [75178-96-O] (0.00040 mol) and cesium carbonate (0.00135 mol) were added and the reaction rnixtare was stirred for 4 hours at 100°C, then overnight at 115°C. Excess of cesium carbonate was removed by filtration. The filtrate was evaporated, yielding intermediate (14). d) Preparation of 4-{2-[6-(3-Armno-propylammo)-pyrido[3,4-d]ρyrimidin-4- ylaminoj-phenoxy} -butyric acid ethyl ester (intermediate 15) Intermediate (14) (0.00055 mol) was dissolved in dichloromethane (11.00 ml).

Trifluoroacetic acid (0.143 mol) was added and the reaction mixture was stirred for 2 hours at room temperature. The solvent and remaining acid were evaporated in the rotary evaporator. The resultant residue (oil) was dried (high- vacuum pump), yielding intermediate (15) (HPLC: 91% P; quantitative yield; used in next reaction step, without further purification). e) Preparation of 4-{2-[6-(3-Ammo-propylammo)-pyrido[3,4-d]pyrimidin-4- ylamino]-phenoxy} -butyric acid (intermediate 16)

Intermediate (15) (0.00055 mol) was dissolved in tefrahyάrofuran (16.00 ml). Water (2.00 ml) was added Lithium hydroxide.monohydrate (0.0055 mol) was added as a solid. More lithium hydroxide.monohydrate was added until a basic pH was reached (until then it was acidic because of CF₃COOH remainders). The reaction mixture was stirred overnight at 65°C. The solvent was evaporated in the rotary evaporator, yielding intermediate (16) (HPLC: 88% P; quantitative yield; used in next reaction step, without further purification).

Example A7 a) Preparation of Allyl-(4-c oro-5-fluoro-2-mtro-benzyl)-me1hyl-amine (intermediate 17) N-methyl-2-propen-l -amine (1.1 equiv) was added to a solution of 4-chloro-S-fluoro-2- nitro-benzaldehyde (1 equiv) in 1,2-dichloroethane (207 ml), thenMgSO₄ (2 spoons) was added and the obtained solution was stirred for 2 hours at room temperature. NaBH(OAc)₃ (3 equiv) was added in 5 portions (one portion per hour) and the reaction mixture was washed with K₂C03- After extraction with CH₂C1₂, the layers were separated. The organic layer was dried over MgSO₄, filtered and evaporated, to afford intermediate (17). b) Preparation of 2-[(Allyl-met3ιyl-ammo)-methyl]-5-c oro-4-fluoro-ρhenylarnine (intermediate 18)

A solution of the nitro derivative intermediate (17) (1 equiv) in a solution of H₂O (120 ml) and NH₄CI (5 equiv) at room temperature was dissolved in Toluene (120 ml), then iron powder (5 equiv) was slowly added and the reaction mixture was stirred and refluxed at 105 °C. The obtained crude was purified by Flash Chromatography. The desired product fractions were collected and the solvent was evaporated, to afford 4.8 g of intermediate (18). c) Preparation of {2-[(Allyl-methyl-amino)-methyl]-5-chloro-4-fluoro-phenyl} -(6- cUoro-pyrido[3,2-^pyrimidώ-4-yl)-amine (intermediate 19)

Triethylamine (3 equiv) was addled to a solution of 4,6-dichloro-pyrido[3,2- t jpyrimidine (1 equiv.) in acetonitrile (dried over Al₂COs) (9 ml). HC1 evolved and the reaction mixture was purged with N₂ for 10 to 15 minutes. Intermediate (18) was added (1.7 equiv.) and then the reaction mixture was stirred and refluxed for 5 hours. After cooling to room temperature, a slightly yellow solid precipitated from the mixture. The product was collected and dried under high vacuum, to yield desired product. EtOAc was added to the mother layer and then a white solid precipitated. After filtration, the filtrate was concentrated and the obtained concentrate was purified by Flash chromatography over silica gel (eluent: Hexane/EtOAc 9/1). The desired fractions were collected and the solvent was evaporated, to yield desired product. Both fractions of desired product were collected, to yield 0.750 g intermediate (19). d) Preparation of N⁵-Allyl-N^-{[2-[(allyl-methyl-amino)-methyl]-5-chloro-4-fluoro- phenyl}-pyrido[3,2-t ιyrimidine-4,6-diamine (intermediate 20)

A solution of intermediate (19) I equiv) in 2-propenylamine (9.8 equiv) was heated overnight in a sealed tube at 10O °C, then the resulting solution was concentrated and dried under high vacuum, to obtain 0.487 g (115 %) of a semi solid that was redissolved in CH₂C12₂. The solution was then filtered and the filtrate was concemtrated again, to afford 0.412 g (100 %) of intermediate (20). e) Preparation of 4,6-ethanediylidenepyrimido[4,5- b] [1 ,4,6, 1 l]benzotetraazacyclotetradecine,l 6-chloro- 15-fluoro-7,8, 11,12,13,18- hexahydro-12-methyl-, (9E)- (intermediate 21) A mixture of intermediate (20) and Grubbs's Catalyst second generation (0.2 equiv) in CH₂C1₂ (7 ml) was stirred and refluxed for 6 hours, then the reaction mixture was stirred for 72 hours at room temperature and refluxed again. An extra amount of B (20 %) was added and then the resulting mixture was stirred and refluxed again for 6 hours. Again extra B (20 %) was added and the mixture was refluxed again overnight. .After concentration, the obtained residue was purified by Flash chromatography over silica gel (eluent: Acetate/Hexane 1/1). The desired fraction were collected and the solvent was evaporated, to yield 0.025 g (38 %) of pure intermediate (21).

B. Preparation of the compounds Example Bl Preparation of 7H,19H-4,6-ethaneά ylidenepyrimido[4,5-b][13,l,4,6]benzoxa- triazacyclopentadecine, 17-chloro-8,9,10,ll,12,13-hexahydro- (compound 1) In two separate dropping funnels, a solution of tributylphosphine (0.00268 mol) in TΗF (20 ml) and a solution of ADDP (0.00155 mol) in TΗF (20 ml) were slowly simultaneously added to a solution of intermediate 2 (0.00103 mol) in TΗF (20 ml) and DMF (2 m) chilled at 0°C under an atmosphere of nitrogen. The reaction mixture was stirred for 4 hours at room temperature, poured out into a IN solution of aqueous hydrochloric acid and after 1 hour, the mixture was diluted with DCM. The precipitate was filtered off, the organic phase was partitioned with a 10% aqueous solution of potassium carbonate, dried (MgSO₄) and concentrated in vacuo. The solid residue was sonicated in hot isopropanol, filtered off, washed with dry ether and dried in vacuo, yielding 0.16g (44%) of compound (1).

Example B2 Preparation of 6,4-(nitrilometheno)pyrimido[4,5-b][13, 1,4,6] benzoxatriazacyclopentadecine, 17-chloro-7,8,9,10,l 1,12,13,19-octahydro- (compound 2) In two separate dropping funnels, a solution of ADDP (0.00102 mol) in TΗF (2 ml) and a solution of tributylphosphine (0.00177 mol) in TΗF (2 ml) were slowly simultaneously added to a solution of intermediate 6 (0.000681 mol) in THF (10 ml) and DMF (1.4 ml), and stirred at room temperature for 18 hours. Then, a solution of ADDP (0.000340 mol) in THF (0.7 mL) and a solution of tributylphosphine (0.000592 mol) in THF (0.7 mL) were simultaneously added at room temperature for 2 hours. The mixture was hydrolyzed and the precipitate was filtered off, wash with water then with, isopropanol and the diethyl ether, and dried in vacuo, yielding 0.124g (49%) of compound (2), melting point >260°C.

Example B3 Preparation of 7H,2 lH-4,6-ethanediylidenepyrimido[4,5-b] [ 15, 1 ,4,6, 10]benzoxa- tetraazacycloheptadecin-12(13H)-one, 8,9,10,11,14,15-hexahydro- (compound 3) 1 - [bis(dime1hylamino)methylene]-3 -oxide- lH-benzotriazolium, hexafluoro- phosphate(l-) [94790-37-1] (0.00057 mol) was dissolved in DMF (20 ml) and stirred at room temperature. Intermediate (11) (0.00019 mol) was dissolved in DMF (10 ml) and N-ethyl-N-(l-methylethyl)- 2-propanamine (0.00114 mol) was added. This solution was added slowly over a 2 hours period to the first solution. The light-green solution was stirred overnight at room temperature. The solvent (DMF) was evaporated. The residue was purified by flash column chromatography, yielding compound (3).

Example B4 Preparation of 7H,2lH-6,4-(nitrilome1heno)pyrimido[5,4-m][l,6,l0,l5]benzoxa- triazacycloheptadecin-12(13H)-one, 8,9,10,11, 14, 15-hexahydro- (compound 4) 1 -[bis((hme ylamino)methylene]-3-oxide-lH-benzotriazolium, hexafluoro- phosphate(l-) [94790-37-1] (0.00165 mol) was dissolved in DMF (40 ml) and stirred at room temperature. Intermediate (16) (0.00055 mol) was dissolved in DMF (20 ml) and N-ethyl-N-(l-methylethyl)-2-propanamine (0.0033 mol) was added. This solution was added slowly over a 2 hours period to the first solution. The light-green solution was stirred overnight at room temperature. The solvent (DMF) was evaporated, yielding compound (4).

All other compounds can be prepared according to these procedures with the remark that the cpds with Y being C₁-₅ alkyl and X²/X^J NH are cyclized under ring closing metathesis conditions using second generation Grubbs catalysts of the dienes (see example B5 hereinafter)

Example B5 Preparation of 4,6-ethanediylidenepyrirnido[4,5- b][l,4,6,l l]benzotetraazacyclotetradecine, 16-chloro-15-fluoro- 7,8,9,10,1 l,12,13,18-octahydro-12-methyl- (compound 5)

Intermediate (21) (1 equiv) was dissolved in a methanol/dioxane mixture (4/1), then catalyst Pt C (0.3 equiv) was added and the reaction mixture was stirred for 4 hours under H₂ atmosphere. The resulting mixture was filtered over a short celite pad and the filtrate was concentrated to dryness. The obtained residue was dried under high vacuum, to afford 0.029 g (60 %) of pure compound (5). Compound identification

The compounds were identified by LC/MS using a gradient elution system on a reversed phase HPLC. The compounds are identified by their specific retention time and their protonated molecular ion MET^1*" peak. The HPLC gradient was supplied by a Waters Alliance HT 2790 system with a columnheater set at 40°C. Flow from the column was split to a Waters 996 photodiode array (PDA) detector and a Waters- Micromass ZQ mass spectrometer with an electrospray ionization source operated in positive and negative ionization mode. Reversed phase HPLC ^was carried out on a Xterra MS C18 column (3.5 μm, 4.6 x 100 mm) with a flow rarte of 1.6 ml/min. Three mobile phases (mobile phase A 95% 25mM ammoniumacetate + 5% acetonitrile; mobile phase B: acetonitrile; mobile phase C: methanol) were employed to run a gradient condition from 100 % A to 50% B and 50% C in 6.5 rminutes, to 100 % B in 1 minute, 100% B for 1 minute and reequilibrate with 100 % A for 1.5 minutes. An injection volume of 10 μL was used.

Mass spectra were acquired by scanning from 100 to 1000 in 1 s using a dwell time of 0.1 s. The capillary needle voltage was 3kN and the source teioperature was maintained at 140°C . Nitrogen was used a the nebulizer gas. Cone voltage was 10 V for positive ionzation mode and 20 V for negative ionization mode. Data acquisition was performed with a Waters-Micromass MassLynx-Openlynx data system.

Table : retention time (RT in minutes) and molecular weight as the MH^""1*"

C. Pharmacological examples

Example C.l : in vitro inhibition of EGFR

The in vitro inhibition of EGFR was assessed using either the Flash Plate technology or the glass-fiber filter technology as described by Davies, S.P. et al., Biochem J. (2000), 351; p.95-105. The Flash Plate technology is generally described by B.A. Brown et al. in High Throughput Screening (1997), p.317-328. Editor(s): Devlin, John P. Pubhsher: Dekker, New York, N. Y.

In the Flash Plate EGFR kinase reaction assay, a kinase substrate consisting of biotinylated poly(L-glutamic acid-L-tyrosine) (poly(GT)biotin), is incubated with the aforementioned protein in the presence of (³³P) radiolabeled ATP. (³³P) phosporylation of the substrate is subsequently measured as light energy emitted using a streptavidin- coated Flash Plate (PerkinElmer Life Sciences) by trapping and quantifying the binding of the biotin tagged and radiolabeled substrate.

Detailed description

The EGFR kinase reaction is performed at 30°C for 60 minutes in a 96-well microtiter FlashPlate (PerkinElmer Life Sciences). For each of the tested compounds a full dose response 1.10^"°M to 1.10^"10M has been performed. IRESSA^® and Tarceva™ (erlotinib) were used as reference compounds. The 100 μl reaction volume contains 54.5 mM TrisHCI pH 8.0, 10 mM MgCI₂, lOOμM Na₃VO₄ , 5.0 μM unlabeled ATP, ImM DTT, 0.009% BSA, 0.8 μCi AT³³P, 0.35 μg/well poly(GT)biotin and 0.5 μg EGFR-kinase domain/well. The reaction is stopped by aspirating the reaction mixture and washing the plate 3x with 200 μl wash/stop buffer (PBS + 100 mM EDTA). After the final wash step 200 μl of wash/stop buffer was added to each well and the amount of phosphorylated (³³P) Poly(GT)biotin determined by counting (30 sec/well) in a microtiteφlate scintillation counter.

In the glass-fiber filter technology EGFR kinase reaction assay, a kinase substrate consisting ofpoly(L-glutamic acid-L-tyrosine) (poly(GT)), is incubated with the aforementioned protein in the presence of (³³P) radiolabeled ATP. (³³P) Phosporylation of the substrate is subsequently measured as radioactivity bound on a glassfiber-filter.

Detailed description

The EGFR kinase reaction is performed at 25°C for 10 minutes in a 96-well microtiteφlate. For each of the tested compounds a full dose response 1.1 Qf^A to 1.10^"

¹⁰M has been performed. IRESSA^® and Tarceva™ (erlotinib) were vised as reference compounds. The 25 μl reaction volume contains 60 mM TrisHCl pHC 7.5, 3 mM

MgCl₂, 3 mM Mn Cl₂ , 3 μM Na₃VO₄ , 50 μg ml PEG20000, 5.0 μM unlabeled ATP, lmM DTT, 0.1 μCi AT³³P, 62.5 ng well poly(GT) and 0.5 μg EGFR-kinase domain/well.

The reaction is stopped by adding 5 μl of a 3% phosphoric acid solution. 10 μl of the reaction mixture is then spotted onto a Filtermat A filter (Wallac) and washed 3 times for 5 min. in 75 mM phosphoric acid and 1 time for 5 min. in methaixol prior to drying and quantification on the Typhoon (Amersham) using a LE phosphorage storage screen. Example C.2: Serum starved proliferation assay on the ovarian carcinoma SKOV3 cells

The ovarian carcinoma cell line (SKOV3) was used in an epidermal growth factor stimulated cell proliferation assay, to assess the inhibitory effect of tine compounds on EGF in whole cells.

In a first step the SKOV3 cells were incubated for 24 hours in the presence of 10% FCS serum. In the second step the cells were incubated with the compounds to be tested in a serum free condition (37 °C and 5% (v/v) CO₂) and subsequently stimulated for 72 hours with EGF at a final concentration of 100 ng/ml. The effect of the compounds on the EGF stimulation was finally assessed in a standard MTT cell viability assay.

The following table provides the pIC50 values of the compounds according to the invention, obtained using the above mentioned kinase assays.

D. Composition examples

The following formulations exemplify typical pharmaceutical compositions suitable for systemic aά ninistration to animal and human subjects in accordance with the present invention. "Active ingredient" (A.I.) as used throughout these examples relates to a compound of formula (T), (XXXI) or a pharmaceutically acceptable addition salt thereof.

Example D.l : film-coated tablets

Prep_.aration_.oftøMet core

A mixture of A.I. (100 g), lactose (570 g) and starch (200 g) was mixed well and thereafter humidified with a solution of sodium dodecyl sulfate (5 g) and polyvinyl- pyrrolidone (10 g) in about 200 ml of water. The wet powder mixture was sieved, dried and sieved again. Then there was added microcrystalline cellulose (100 g) and hydrogenated vegetable oil (15 g). The whole was mixed well and compressed into tablets, giving 10.000 tablets, each comprising 10 mg of the active ingredient. Coating

To a solution of methyl cellulose (10 g) in denaturated ethanol (75 ml) there was added a solution of ethyl cellulose (5 g) in CH₂C1₂ (150 ml). Then there were added CH₂C1₂ (75 ml) and 1,2,3-propanetriol (2.5 ml). Polyethylene glycol (10 g) was molten and dissolved in dichloromethane (75 ml). The latter solution was added to the former and then there were added magnesium octødecanoate (2.5 g), polyvinyl-pyrrolidone (5 g) and concentrated color suspension (30 ml) and the whole was homogenated. The tablet cores were coated with the thus obtained mixture in a coating apparatus.

Claims

1. A compound having the formula

a¹-a²=a³-a⁴ represents a divalent radical selected from Ν-CH=CH-CH, N-CH=N-CH orCH-CH=N-CH; Z represents O, NH or S;

Y represents -C_3-9alkyl-, -C_3-9alkenyl-, -Ci-salkyl-oxy-Ci-salkyl-, -Ci-salkyl-NR^-Ci.salkyl-^Ci-salkyl-NR^-CO-Ci.salkyl-, -Ci-salkyl-CO-NR^-Ci-salkyl-^Ci^alkyl-CO-NH-, -Ci-ealkyl-NH-CO-, -CO-NH-Ci-ealkyl-, -NH-CO-C_1-6alkyl-, -CO-Cι_-7alkyl-, -Cι_-7alkyl-CO-, Ci-ealkyl-CO-Ci-ealkyl;

X¹ represents a direct bond, O, -O-Cι_-2alkyl-, CO, -CO- C_1-2alkyl-, NR¹¹, -NRⁿ-Cι.₂alkyl-, NR¹⁶-CO-, NR¹⁶-CO-Cι_-2alkyl-, -O-N=CH- or Cι_₂alkyl; X² represents a direct bond, O, -O-Cι_-2alkyl-, CO, -CO- Cι_-2alkyl-, NR¹², NR¹²-Cι_-2alkyl-, NR¹⁷-CO-, NR¹⁷-CO-Cι_-2alkyl-, Het²⁰-Cι_-2alkyl-, -O-N=CH- or C_1-2alkyl;

R¹ represents hydrogen, cyano, halo, hydroxy, formyl, Cι_₆alkoxy-,

Cι-₆alkoxy- substitated with halo, substituted with one or where possible two or more substitaents selected from hydroxy or halo; R² represents hydrogen, cyano, halo, hydroxy, hydroxycarbonyl-, Het¹⁶-carbonyl-,

aminocarbonyl-, mono-or

Het¹, formyl, C_1- alkyl-, C_2-6alkynyl-, C₃-₆cycloalkyl- C3-6cycloalkyloxy-,

Ar⁵, Ar^x-oxy-, dihydroxyborane , Ci-βalkoxy- substituted with halo, substituted with one or where possible two or more substitaents selected from halo, hydroxy or NR⁵R⁶, Ci-4alkylcarbonyl- wherein said C_1-4alkyl is optionally substituted with one or where possible two or more substitaents selected from hydroxy or

R³ represents hydrogen, CMalkyl, cyano or C_Malkyl substituted with one or more substituents selected from halo, C_halky loxy-, amino-, mono-or

or phenyl; R⁴ represents hydrogen, hydroxy, Ar³-oxy, Ar⁴-C_1-4alkyloxy-,

C_2-4alkenyloxy- optionally substitated with Het¹² or R⁴ represents

substituted with one or where possible two or more substitaents selected from

R⁵ and R⁶ are each independently selected from hydrogen or

R⁷ andR⁸ are each independently selected from hydrogen, C_Malkyl, Het⁸, arninosulfonyl-, mono- or di

C₃-₆cycloalkyl, Het⁹- carbonyl-Cι-₄a!kyl-, Het¹⁰-carbonyl-,

Het¹¹ -C_Malkyl- or

R⁹ andR¹⁰ are each independently selected from hydrogen, C_Malkyl, C₃^cycloalkyl, Het⁴,

or polyhydroxy-Cι_4alkyl-;

R¹¹ represents hydrogen, C_Malkyl, Het⁵,

C₂.₄alkenylcarbonyl- optionally substituted with

C₂-4alkenylsulfonyl-,

or phenyl optionally substitated with one or where possible two or more substitaents selected from hydrogen, hydroxy, amino or

R¹² represents hydrogen, CMalkyl, C_1- aIkyl-oxy-carbonyl-, Het¹⁷, Het¹⁸-Cι_4alkyl-, C_2- alkenylcarbonyl- optionally substituted with

or phenyl optionally substituted witto. one or where possible two or more substitaents selected from hydrogen, hydroxy, amino or C^alkyloxy-; R¹³ represents hydrogen, CMalkyl, Het¹³, Het¹⁴-C_1-4alkyl- or phenyl optionally substituted with one or where possible two or more substitaents selected from hydrogen, hydroxy, amino or Ci-4alkyloxy-; R¹⁴ andR¹⁵ are each independently selected from hydrogen,

R¹⁶ and R¹⁷ are each independently selected from hydrogen,

Het²¹-Cι- alkyl-

Het¹ represents a heterocycle selected from piperidinyl, moφholinyl, piperazinyl, furanyl, pyrazolyl, dioxolanyl, thiazolyl, oxazolyl, imidazolyl, isoxazolyl, oxadiazolyl, pyridinyl or pyrrolidinyl wherein said Het¹ is optionally substitated with one or where possible two or more substitaents selected from amino,

mono- or di(C_1- alkyl)amino- or amino-carbonyl-; Het² represents a heterocycle selected from moφholinyl, piperazinyl, piperidinyl, pyrrolidinyl, thiomoφholinyl or dithianyl wherein said Het² is optionally substitated with one or where possible two or more substitaents selected from hydroxy, halo, amino, C_halky!-,

sulfonyl-, aminosulfonyl-; Het³, Het⁴ and Het⁸ each independently represent a heterocycle selected from moφholinyl, piperazinyl, piperidinyl, furanyl, pyrazolyl, dioxolanyl, thiazolyl, oxazolyl, imidazolyl, isoxazolyl, oxadiazolyl, pyridinyl or pyrrolidinyl wherein said Het³, Het⁴ or Het⁸ is optionally substitated with one or where possible two or more substitaents selected from hydroxy-, amino-,

Cs-ecycloalkyl-C^alkyl-, aminosulfonyl-, mono- or di(Cι^alkyl)aminosulfonyl or

Het⁵ represent a heterocycle selected from pyrrolidinyl or piperidinyl wherein said heterocycle is optionally substitated with one or where possible two or more substitaents selected from

C_3-6cycloalkyl,

Het⁶ and Het⁷ each independently represent a heterocycle selected from moφholinyl, pyrrolidinyl, piperazinyl or piperidinyl wherein said Het⁶ and Het⁷ are optionally substitated with one or where possible two or more substitaents selected from C_3-6cycloalkyl, hydroxy-Cι_-4alkyl-,

or

Het⁹ and Het¹⁰ each independently represent a heterocycle selected from furanyl, piperidinyl, moφholinyl, piperazinyl, pyrazolyl, dioxolanyl, thiazolyl, oxazolyl, imidazolyl, isoxazolyl, oxadiazolyl, pyridinyl or pyrrohdinyl wherein said Het⁹ or Het¹⁰ is optionally substitated C_1-4alkyl, or

Het¹¹ represents a heterocycle selected from i

ndolyl or Het¹² represents a heterocycle selected from moφholinyl, piperazinyl, piperidinyl, pyrrolidinyl, thiomoφholinyl or dithianyl wherein said Het¹² is optionally substitated with one or where possible two or more substitaents selected from hydroxy, halo, amino,

hydroxy-Ci^alkyl-oxy-Ci^alkyl-, mono- or di(C_1- aIkyι)amino- or mono- or ch(C_1- alkyl)ammo-Cι. alkyl-; Het¹³ represent a heterocycle selected from pyrrolidinyl or piperidinyl wherein said heterocycle is optionally substitated with one or where possible two or more substitaents selected from

C_3-6cycloalkyl,

Het¹⁴ represent a heterocycle selected from moφholinyl, pyrrolidinyl, piperazinyl or piperidinyl wherein said heterocycle is optionally substitated with one or where possible two or more substitaents selected from

or polyhydroxy-Cι_₄alkyl-; Het¹⁵ and Het²¹ each independently represent a heterocycle selected from moφholinyl, pyrrohdinyl, piperazinyl or piperidinyl wherein said Het¹⁵ or Het²¹ are optionally substitated with one or where possible two or more substitaents selected from Ci. alkyl, C₃-₆Cycloalkyl,

or polyhydroxy- Cι-4alkyl-; Het¹⁶ represent a heterocycle selected from moφholinyl, pyrrolidinyl, piperazinyl, 1,3,2-dioxaborolane or piperidinyl wherein said heterocycle is optionally substitated with¹ one or more substituents selected from

^"*

Het¹⁷ represent a heterocycle selected from pyrrolidinyl or piperidinyl wherein said heterocycle is optionally substituted with one or where possible two or more substitaents selected from

Het¹⁸ and Het¹⁹ each independently represent a heterocycle selected from moφholinyl, pyrrolidinyl, piperazinyl or piperidinyl wherein said Het¹⁸ and Het¹⁹ are optionally substitated with one or where possible two or more substitaents selected from C₃.₆Cycloalkyl, hydroxy-C_1- alkyl-,

or polyhydroxy-C_Malkyl-; Het²⁰ represents a heterocycle selected from pyrrolidinyl, 2-pyrrolidinyl, piperidinyl, piperazinyl or pyrazolidinyl wherein said heterocycle is optionally substituted with one or where possible two or more substitaents selected from C_1-4alkyl, C₃-₆cycloalkyl,

or

Ar¹, Ar², Ar³, Ar⁴ and Ar⁵ each independently represent phenyl optionally substituted with cyano,

C_1- aIkylsulfonylamino-, ammosulfonylamino-,

2. A compound according to claim 1 wherein; Z represents NH; Y represents -C_3-9alkyl-, -C_2-9alkenyl-, -Ci-salkyl-oxy-Ci-salkyl-, -C_1-5alkyl-NR¹³-C_1-5alkyl-, -C_1-5alkyl-NR¹⁴-CO-Cι_-5alkyl-,

- CO-Cι_-7alkyl-, -Cι_-7alkyl-CO- or Ci-ealkyl-CO-Ci-ealkyl; X¹ represents O, -O-Cι_-2alkyl-, -O-N=CH-, NR¹¹ or -NR^π-Cι_-2alkyl-; in a particular embodiment X¹ represents a direct bond, Cι_-2alkyl-,

-O- or -O-CH₂-; X² represents a direct bond, O, -O-Cι_-2alkyl-, -O-N=CH-, NR¹⁷-CO-, NR¹⁷-CO-Cι_-2alkyl-, Cι_-2alkyl, Het²⁰-C_1-2alkyl-, NR¹² or NR¹²-Cι.₂alkyl-; in a particular embodiment X² represents a direct bond, C_halky!-, -O-C_1-2alkyl, NR¹⁷-CO-, NR¹⁷-CO-Cι_-2alkyl-, Het²⁰-Cι_-2alkyl-, -O- or-O-CH₂-; R¹ represents hydrogen, cyano, halo or hydroxy, preferably halo; R² represents hydrogen, cyano, halo, hydroxy, hydroxycarbonyl-,

Het¹⁶-carbonyl-,

C_2-6alkynyl-, Ar⁵ or Het¹; in a further embodiment R² represents hydrogen, cyano, halo, hydroxy, or Ar⁵; in a more particular embodiment R² represents hydrogen or halo; R³ represents hydrogen;

R⁴ represents hydrogen, hydroxy, Cι-4alkyloxy-, Ar⁴-Ci_-4alkyloxy or R⁴ represents Cι-4aIkyloxy substitated with one or where possible two or more substitaents selected from

R¹¹ represents hydrogen, C_1- alkyl- or Ci^alkyl-oxy-carbonyl-; R¹² represents hydrogen, C_1- alkyl- or Ci^alkyl-oxy-carbonyl-; R¹³ represents hydrogen or in particular moφholinyl-C_Malkyl; R¹⁴ represents hydrogen or

R¹⁷ represents hydrogen,

in particular R¹⁷ represents hydrogen or

Het¹ represents thiazolyl optionally substitated with arnino, C_1- alkyl, hydroxy-Ci- ₄alkyl-, phenyl,

mono- or di(Cι. ₄alkyl)amino- or amino -carbonyl- ; Het² represents a heterocycle selected from moφholinyl, piperazinyl, piperidinyl or pyrrolidinyl wherein said Het² is optionally substitated with one or where possible two or more substituents selected from hydroxy, amino or C^alkyl-; In a further embodiment Het² represents a heterocycle selected from moφholinyl or piperidinyl optionally substituted with

preferably methyl;

Het¹⁴ represents a heterocycle selected from moφholinyl, piperazinyl, piperidinyl or pyrrolidinyl wherein said Het¹⁴ is optionally substitated with one or where possible two or more substitaents selected from hydroxy, amino or

Het¹⁶ represents a heterocycle selected from piperidinyl, moφholinyl or pyrrolidinyl; Het²⁰ represents a heterocycle selected from pyrrolidinyl, 2-pyrrohdinyl or piperidinyl;

Het²¹ represents a heterocycle selected from moφholinyl, piperazinyl, piperidinyl or pyrrolidinyl wherein said Het²¹ is optionally substitated with one or where possible two or more substitaents selected from hydroxy, amino or

Ar⁴ represents phenyl optionally substituted with cyano, hydro

or CMalkyl;

Ar⁵ represents phenyl optionally substitated with cyano, hydroxy,

or

3. A compound according to claim 1 wherein; Z represents NH;

Y represents -C_3-9alkyl-,

-Cι_-5alkyl-NR¹⁴-CO-C_1-5alkyl-, - Ci-ealkyl-NH-CO- or -CO-NH -Ci-ealkyl- ; X¹ represents -O-, -NR¹¹-, -NR¹⁶-CO-, or -NR¹⁶-CO-Cι_₂alkyl-; X² represents a direct bond, -C_1-2alkyl-, -O-Cι_-2alkyl, -O-, -O-CH₂- or He -Cι_-2alkyl-; R¹ represents hydrogen or halo; R² represents hydrogen, cyano, halo, hydroxycarbonyl-,

Het¹⁶-carbonyl- or Ar⁵; in particular R² represents hydrogen or halo; R³ represents hydrogen; R⁴ represents hydrogen, hydroxy, Cι-4alkyloxy-, Ar⁴-C₁-₄alkyloxy or R⁴ represents C_1- alkyloxy substituted with one or where possible two or more substitaents selected from C_1-4alkyloxy- or Het²-;

R¹¹ represents hydrogen; R ,12 represents hydrogen, C_1- alkyl- or

R¹³ represents hydrogen or

in particular hydrogen or

preferably methyl; Het¹⁴ represents moφholinyl; Het¹⁶ represents a heterocycle selected from moφholinyl or pyrrolidinyl; Het²⁰ represents pyrrolidinyl or piperidinyl; Ar⁴ represents phenyl; Ar⁵ represents phenyl optionally substituted with cyano.

4. A compound according to claim 1 or 2 wherein the R¹ substituent is at position 4', tthhee RR²² ssuubbssttiittuueenntt iiss aatt ppoossiittiioonn 55'',, t thhee RR³³ ssuubbssttiittuueenntt iiss at position 3 and the R⁴ substituent at position 7 of the structure of formula (I).

5. A compound according to any one of claims 1 to 4 wherein a^α-a²=a³-a⁴ represents N-CH=CH-CH

6. A compound according to any one of claims 1 to 4 wherein a¹-a²=^a³-a⁴ represents N-CH=N-CH.

7. A compound according to any one of claims 1 to 4 wherein a^x-a²=^a³-a⁴ represents CH-CH=N-CH

8. An intermediate of formula

„ the N-oxide forms, the pharmaceutically acceptable addition salts and the stereochemically isomeric forms thereof, wherein a¹-a²=a³-a⁴ represents a divalent radical selected from N-CH=CH-CH or N-CH=N-CH; Y represents -C_3-9alkyl-,

-C_walkyl-NH-CO- or -CO-NH -Ci-salkyl- ; R¹ represents hydrogen or halo; R² represents hydrogen, cyano, halo, hydroxycarbonyl-,

Het¹⁶-carbonyl- or Ar⁵; R⁴ represents hydroxy, C_1- alkyloxy-,

or R⁴ represents

substituted with one or where possible two or more substitaents selected from

R • π represents hydrogen;

R¹³ represents Het¹⁴-Cι-₄alkyl;

Het² represents a heterocycle selected from moφholinyl, piperazinyl, piperidinyl or pyrrolidinyl wherein said Het² is optionally substituted with one or where possible two or more substitaents selected from hydroxy, amino or C_1- alkyl-;

Het¹⁴ represents moφholinyl;

Het¹⁶ represents a heterocycle selected from moφholinyl or pyrrolidinyl;

Ar⁴ represents phenyl;

Ar⁵ represents phenyl optionally substituted with cyano.

9. A kinase inhibitor of formula (I) or formula (XXXI).

10. A compound as claimed in any one of claims 1 to 7 for use as a medicine.

11. Use of a compound as claimed in any one of claims 1 to 7 in the manufacture of a medicament for treating cell proliferative disorders such as atherosclerosis, restenosis and cancer.

12. A pharmaceutical composition comprising a pharmaceutically acceptable carrier and, as active ingredient, an effective kinase inhibitory amount of a compound as described in any one of the claims 1 to 7.

13. An intermediate as claimed in claim 8 for use as a medicine.

14. Use of an intermediate as claimed in claim 8 in the manufacture of a medicament for treating cell proliferative disorders such as atherosclerosis, restinosis and cancer.

15. A pharmaceutical composition comprising a pharmaceutically acceptable carrier and, as active ingredient, an effective kinase inhibitory amount of an intermediate as claimed in claim 6.

16. A process for preparing a compound as claimed in claims 1 to 7, comprising; a) coupling 2-acetoxy-8-cMoropyrimido[5,4-d]pyrimidine derivatives (D) with suitable substitated anilines (ET), to furnish the intermediates of formula (IN), and deprotecting the intermediates of formula (TV) followed by ring closure under suitable conditions.

Deprotection

V = protective group

; or b) coupling the known 8-c oro-2(methylt o)-pyrimido[5,4-d]pyrimidine with 2- aminophenol derivatives of formula (XXI), yielding the intermediate compounds of formula (XXII). Next, the pyrido[3,2-d]pyrimidme of formula (XXD) is aminated using an aminated alcohol (XXID) under art known conditions, followed by ring closure under Mitsunobu conditions to give the target compounds of formula (I")

H Cx πi

17. A method of treating a cell proliferative disorder, the method comprising administering to an animal in need of such treatment a therapeutically effective amount of a compound as claimed in any one of claims 1 to 7.

18. A method of treating a cell proliferative disorder, the method comprising aclministering to an animal in need of such treatment a therapeuticaUy effective amount of an intermediate as claimed in claim 8.