WO2005040100A1 - Tetrahydro-naphthalene and urea derivatives - Google Patents

Tetrahydro-naphthalene and urea derivatives Download PDF

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Publication number
WO2005040100A1
WO2005040100A1 PCT/EP2004/011008 EP2004011008W WO2005040100A1 WO 2005040100 A1 WO2005040100 A1 WO 2005040100A1 EP 2004011008 W EP2004011008 W EP 2004011008W WO 2005040100 A1 WO2005040100 A1 WO 2005040100A1
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WO
WIPO (PCT)
Prior art keywords
amino
halogen
alkyl
hydroxy
alkoxycarbonyl
Prior art date
Application number
PCT/EP2004/011008
Other languages
French (fr)
Inventor
Axel Bouchon
Nicole Diedrichs
Achim Hermann
Klemens Lustig
Heinrich Meier
Josef Pernerstorfer
Elke Reissmüller
Muneto Mogi
Takeshi Yura
Hiroshi Fujishima
Masanori Seki
Yuji Koriyama
Kayo Yasoshima
Keiko Misawa
Masaomi Tajimi
Noriyuki Yamamoto
Klaus Urbahns
Fumihiko Hayashi
Yasuhiro Tsukimi
Jang Gupta
Original Assignee
Bayer Healthcare Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bayer Healthcare Ag filed Critical Bayer Healthcare Ag
Priority to US10/575,027 priority Critical patent/US20080045546A1/en
Priority to CA002542494A priority patent/CA2542494A1/en
Priority to JP2006534634A priority patent/JP2007509846A/en
Priority to EP04765763A priority patent/EP1678123A1/en
Publication of WO2005040100A1 publication Critical patent/WO2005040100A1/en

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    • C07C275/00—Derivatives of urea, i.e. compounds containing any of the groups, the nitrogen atoms not being part of nitro or nitroso groups
    • C07C275/28—Derivatives of urea, i.e. compounds containing any of the groups, the nitrogen atoms not being part of nitro or nitroso groups having nitrogen atoms of urea groups bound to carbon atoms of six-membered aromatic rings of a carbon skeleton
    • C07C275/32—Derivatives of urea, i.e. compounds containing any of the groups, the nitrogen atoms not being part of nitro or nitroso groups having nitrogen atoms of urea groups bound to carbon atoms of six-membered aromatic rings of a carbon skeleton being further substituted by singly-bound oxygen atoms
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    • A61K31/16—Amides, e.g. hydroxamic acids
    • A61K31/165—Amides, e.g. hydroxamic acids having aromatic rings, e.g. colchicine, atenolol, progabide
    • A61K31/167—Amides, e.g. hydroxamic acids having aromatic rings, e.g. colchicine, atenolol, progabide having the nitrogen of a carboxamide group directly attached to the aromatic ring, e.g. lidocaine, paracetamol
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    • A61K31/17—Amides, e.g. hydroxamic acids having the group >N—C(O)—N< or >N—C(S)—N<, e.g. urea, thiourea, carmustine
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    • C07D213/04—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom
    • C07D213/24—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom with substituted hydrocarbon radicals attached to ring carbon atoms
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    • C07D213/04—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom
    • C07D213/24—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom with substituted hydrocarbon radicals attached to ring carbon atoms
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    • C07D213/89—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members with hetero atoms directly attached to the ring nitrogen atom
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    • C07D239/72—Quinazolines; Hydrogenated quinazolines
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    • C07D261/10—Heterocyclic compounds containing 1,2-oxazole or hydrogenated 1,2-oxazole rings not condensed with other rings having two or more double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
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    • C07C2602/04—One of the condensed rings being a six-membered aromatic ring
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    • C07C2602/10—One of the condensed rings being a six-membered aromatic ring the other ring being six-membered, e.g. tetraline

Definitions

  • the present invention relates to a tetrahydro-naphthalene or an urea derivative which is useful as an active ingredient of pharmaceutical preparations.
  • the tetrahydro-naphthalene and urea derivatives of the present invention have vanilloid receptor (VR1) antagonistic activity, and can be used for the prophylaxis and treatment of diseases associated with VR1 activity, in particular for the treatment of urological diseases or disorders, such as detrusor overactivity (overactive bladder), urinary incontinence, neurogenic detrusor oeractivity (detrusor hyperflexia), idiopathic detrusor overactivity (detrusor instability), benign prostatic hype ⁇ lasia, and lower urinary tract symptoms; chronic pain, neuropathic pain, postoperative pain, rheumatoid arthritic pain, neuralgia, neuropathies, algesia, nerve injury, ischaemia, neurodegeneration, stroke, and inflammatory disorders such as asthma and chronic obstructive pulmonary (or
  • Vanilloid compounds are characterized by the presence of vanillyl group or a functionally equivalent group.
  • vanilloid compounds or vanilloid receptor modulators are vanillin (4-hydroxy-3-methoxy-benzaldehyde), guaiacol (2-methoxy-phenol), zingerone (4-/4-hydroxy-3- methoxyphenyl/-2-butanon), eugenol (2-methoxy4-/2-propenyl/phenol), and capsaicin (8-methy-N- vanillyl-6-noneneamide).
  • capsaicin the main pungent ingredient in "hot” chili peppers, is a specific neurotoxin that desensitizes C-f ⁇ ber afferent neurons.
  • Capsaicin interacts with vanilloid receptors (VR1), which are predominantly expressed in cell bodies of dorsal root ganglia (DRG) or nerve endings of afferent sensory fibers including C-fiber nerve endings [Tominaga M, Caterina MJ, Malmberg AB, Rosen TA, Gilbert H, Skinner K, Raumann BE, Basbaum Al, Julius D: The cloned capsaicin receptor integrates multiple pain-producing stimuli. Neuron. 21: 531-543, 1998].
  • VR1 vanilloid receptors
  • the VR1 receptor was recently cloned [Caterina MJ, Schumacher MA, Tominaga M, Rosen TA, Levine JD, Julius D: Nature 389: 816-824, (1997)] and identified as a nonselective cation channel with six transmembrane domains that is structurally related to the TRP (transient receptor potential) channel family. Binding of capsaicin to VRl allows sodium, calcium and possibly potassium ions to flow down their concentration gradients, causing initial depolarization and release of neurotransmitters from the nerve terminals. VRl can therefore be viewed as a molecular integrator of chemical and physical stimuli that elicit neuronal signals in pathological conditions or diseases.
  • antagonists of the VRl receptor can be used for prophylaxis and treatment of the conditions and diseases including chronic pain, neuropathic pain, postoperative pain, rheumatoid arthritic pain, neuralgia, neuropathies, algesia, nerve injury, ischaemia, neuro- degeneration, stroke, inflammatory disorders, urinary incontinence (UI) such as urge urinary incontinence (UUI), and/or overactive bladder.
  • UI urinary incontinence
  • UUI urge urinary incontinence
  • UUI is the involuntary loss of urine.
  • UUI is one of the most common types of UI together with stress urinary incontinence (SUI) which is usually caused by a defect in the urethral closure mechanism.
  • UUI is often associated with neurological disorders or diseases causing neuronal damages such as dementia, Parkinson's disease, multiple sclerosis, stroke and diabetes, although it also occurs in individuals with no such disorders.
  • One of the usual causes of UUI is overactive bladder (OAB) which is a medical condition refening to the symptoms of frequency and urgency derived from abnormal contractions and instability of the detrusor muscle.
  • OAB overactive bladder
  • Orally active anticholinergic drugs which are commonly prescribed, such as propantheline (ProBanthine), tolterodine tartrate (Detrol) and oxybutynin (Difropan), have serious drawbacks such as unacceptable side effects such as dry mouth, abnormal visions, constipation, and central nervous system disturbances. These side effects lead to poor compliance. Dry mouth symptoms alone are responsible for a 70% non-compliance rate with oxybutynin. The inadequacies of present therapies highlight the need for novel, efficacious, safe, orally available drugs that have fewer side effects.
  • WO03/014064 discloses the compounds represented by the general formula:
  • X represents C 3-8 cycloalkyl optionally fused by benzene, optionally substituted naphthyl, optionally substituted phenyl, optionally substituted phenyl C I-6 straight alkyl, phenyl fused by cycloalykyl, etc;
  • Q aa represents CH or N
  • R aa represents hydrogen or methyl
  • R b represents hydrogen or methyl
  • Y represents substituted naphthyl
  • WO03/022809 discloses the compounds having vanilloid receptor antagonist activity represented by the general formula:
  • P and P' independently represent aryl or heteroaryl
  • R and R independently represent hydrogen, alkoxy, hydroxy, etc;
  • WO03/053945 discloses the compounds having vanilloid receptor antagonist activity represented by the general formula:
  • R represents hydrogen, alkoxy, hydroxy, etc.
  • R represents
  • WO03/070247 discloses the compounds having vanilloid receptor antagonist activity represented by the general formula:
  • Xc represents N or CR cl ;
  • Xc 2 represents N or CR c2 ;
  • Xc 3 represents N, NR c3 or CR c3 ;
  • Xc 4 represents a bond, N or CR° 4 ;
  • Xc 5 represents N or C; provided that at least one of Xci, Xc 2 , Xc 3 and Xc 4 is N;
  • Zc_ represents O, NH or S;
  • Zc 2 represents a bond, NH or S;
  • L c represents alkylene, cycloalkylene, etc;
  • R cl , R c2 , R c3 , R c4 , R c5 , R c6 , R c7 , R c8a R cSb are defined in the application; and
  • R c9 represents hydrogen, aryl, cycloalkyl, and heterocylcle.
  • WO03/080578 discloses the compounds having vanilloid receptor antagonist activity represented by the general formula:
  • a d , B d , D and E d are each C or N with the proviso that one or more are N;
  • Y ⁇ is an aryl, heteroaryl, carbocyclyl or tased-carbocyclyl;
  • n is 0, 1, 2 or 3; and R", R" , R , R a4 ,
  • R and R > d6 are defined in the application.
  • This invention is to provide a compound of the formula (A), their tautomeric and stereoisomeric form, and salts thereof:
  • This invention is to provide an urea derivative of the formula (I), their tautomeric and stereo- isomeric form, and salts thereof:
  • n represents an integer of 0 to 6;
  • Q] and Q independently represent direct bond or methylene
  • Chemical bond between Q 2 __r__rQ 3 is selected from the group consisting of a single bond and a double bond; when Q 2. __z_ .
  • Q 3 is a single bond, Q 2 represents CHR 2 , or CO, and Q 3 represents CHR 3 , when Q, — Q, is a double bond, Q 2 represents CR 2 and Q 3 represents CR 3 ;
  • R 2 represents hydrogen, hydroxy, C ⁇ . 6 alkoxy or C ⁇ -6 alkanoyloxy
  • R 3 represents hydrogen, hydroxy, C 1-6 alkoxy, C ⁇ -6 alkanoyloxy, or C 1-6 alkyl optionally substituted by hydroxy, C_. 6 alkoxy or C__ 6 alkanoyloxy, with the proviso that Qj and Q can not be direct bond at the same time;
  • R 2 and R 3 can not be hydrogen at the same time; when Qi and Q 4 are both methylene and R 3 is hydroxy, R 2 is hydroxy, C__ 6 alkoxy or C ⁇ - 6 alkanoyloxy; when Q . is direct bond, R 2 is hydroxy, C ⁇ . 6 alkoxy or C_ -6 alkanoyloxy; and when Q 4 is direct bond, R 2 is hydrogen, C_ -6 alkoxy or Cj._ 6 alkanoyloxy; and R 4 represents aryl optionally having one or two substitaents selected from the group consisting of halogen, hydroxy, .
  • the urea derivatives of formula (I) are those wherein; Qi and Q 4 represent methylene;
  • Q 2 represents CHR 2 , or CO, wherein
  • R 2 represents hydroxy, C ⁇ -6 alkoxy or C 1-6 alkanoyloxy; and Q 3 represents CHR 3 , wherein
  • R 3 represents hydrogen, hydroxy, C ⁇ . 6 alkoxy or C ⁇ -6 alkanoyloxy.
  • urea derivatives of formula (I) are those wherein;
  • Q] represents methylene; Q 4 represents direct bond;
  • Q 2 represents CHR 2 or CO, wherein
  • R 2 represents hydrogen, C ⁇ . 6 alkoxy or C_.6 alkanoyloxy; and Q 3 represents CHR 3 , wherein
  • R 3 represents hydrogen, hydroxy, C 1-6 alkoxy or C ⁇ -6 alkanoyloxy, with the proviso that R 2 and R 3 can not be hydrogen at the same time.
  • urea derivatives of formula (I) are those wherein; Qi represents direct bond;
  • Q 4 represents methylene; O — 0 ⁇ is a single bond; Q 2 represents CHR 2 or CO, wherein R 2 represents hydroxy, C ⁇ -6 alkoxy or C ⁇ _ 6 alkanoyloxy;
  • Q 3 represents CHR 3 , wherein
  • R 3 represents hydrogen, hydroxy, C ⁇ _ 6 alkoxy or C_ -6 alkanoyloxy.
  • the urea derivatives of formula (T) are those wherein; Qi and Q represent methylene;
  • Q- — Q- is a double bond
  • Q 2 represents CR 2 , wherein
  • R 2 represents C ⁇ _ 6 alkoxy or C ⁇ -6 alkanoyloxy
  • Q 3 represents CR 3 , wherein R 3 represents hydrogen, C ⁇ -6 alkoxy or C_. 6 alkanoyloxy.
  • the urea derivatives of formula (I) are those wherein;
  • Q?— -0? is a single bond or a double bond; when Q 2 r . z_ . Q 3 is a single bond, Q 2 represents CH 2 and Q 3 represents CHR 3 , and when Q 2 _ _rQ 3 is a double bond, Q 2 represents CH and Q 3 represents CR 3 , wherein
  • R 3 represents C_ -6 alkyl optionally substituted by hydroxy.
  • the urea derivatives of formula (I) are those wherein; n represents an integer of 0 to 1; and R 4 represents phenyl optionally substitated with one or more substitaents selected from the group consisting of chloro, bro o, fluoro, nitro, mthoxy, trifluoromethyl and trifluoromethoxy.
  • said urea derivative of the formula (I) is selected from the group consisting of:
  • This invention is to provide a hydroxy-tefrahydro-naphthalene derivatives of the formula (I), their tautomeric and stereoisomeric form, and salts thereof:
  • n represents an integer of 0 to 6;
  • R 1 represents C 3 _ 8 cycloalkyl optionally fused by aryl, wherein said aryl is optionally substitated with one or more substitaents selected from the group consisting of halogen, hydroxy, carboxy, nitro, cyano, amino, C ⁇ _ 6 alkylamino, di(C ⁇ -6 alkyl)amino, C_ -6 alkoxycarbonyl, C__ 6 alkanoyl, C ⁇ -6 alkanoylamino, carbamoyl, C ⁇ -6 alkylcarbamoyl, C ⁇ -6 alkyl optionally substituted by cyano, C_ -6 alkoxycarbonyl, or mono-, di-, or tri- halogen, C .
  • .6 alkoxy optionally substitated by mono-, di-, or tri- halogen and C].
  • 6 alkylthio optionally substitated by mono-, di-, or tri- halogen; phenyl substitated by heteroaryl, or heteroaryloxy, wherein said heteroaryl and heteroaryloxy are optionally substituted with one or more substitaents selected from the group consisting of halogen, hydroxy, carboxy, nitro, cyano, amino, C_.6 alkylamino, di(C_ -6 alkyl)amino, C_. 6 alkoxycarbonyl, Q.
  • hydroxy-tetrahydro-naphthalenylurea derivatives of formula (I) can be those wherein; n represents an integer of 0 or 1 ; and
  • R 1 represents C 5- 6cycloalkyl optionally fused by benzene, pyridine, or pyrimidine, wherein said benzene, pyridine, and pyrimidine are optionally substitated by halogen, nitro, or C ⁇ - 6 alkyl optionally substitated by mono-, di-, or tri-halogen.
  • hydroxy-tefrahydro-naphthalenylurea derivatives of formula (I) can be those wherein; n represents an integer of 0 or 1; and
  • R 1 represents phenyl substituted by thienyl, furyl, pynolyl, thiazolyl, oxazolyl, isoxazolyl, imidazolyl, pyridyl, pyrimidyl, friazolthiadiazolyl, thienyloxy, furyloxy, pynolyl, thiazolyloxy, oxazolyloxy, isoxazolyloxy, imidazolyloxy, pyridyloxy or pyrimidyloxy, wherein said thienyl, furyl, pynolyl, thiazolyl, oxazolyl, isoxazolyl, imidazolyl, pyridyl, pyrimidyl, friazolthiadiazolyl, thienyloxy, furyloxy, pynolyl, thiazolyloxy, oxazolyloxy, isoxazolyloxy, imid
  • hydroxy-tetrahydro-naphthalenylurea derivatives of formula (T) can be those wherein; n represents an integer of 0 or 1 ; and
  • R 1 represents phenyl fused with thiophene, furan, pyrrole, thiazole, oxazole, isoxazole, imidazole, pyridine, pyrimidine, 1,3-dioxalane, tetrahydrofuran. pynolidine, piperidine, or mo ⁇ holine.
  • thiophene, furan, pyrrole, thiazole, oxazole, isoxazole, imidazole, pyridine and pyrimidine are optionally substitated with one or more substitaents selected from the group consisting of halogen, nitro, C ⁇ -6 alkyl optionally substitated by mono-, di-, or tri-halogen, - ⁇ alkoxy optionally substitated by mono-, di-, or tri- halogen, and C ⁇ -6 alkylthio optionally substitated by mono-, di-, or tri- halogen.
  • hydroxy-tetrahydro-naphthalenylurea derivatives of formula (I) can be those wherein; n represents an integer of 0 or 1 ; and
  • R 1 represents phenyl fused with 1 ,3-dioxalane or tetrahydrofuran.
  • the hydroxy-tetrahydro-naphthalenylurea derivatives of formula (I) can be those wherein; n represents an integer of 0 or 1 ;
  • R 1 represents thienyl, furyl, pynolyl, thiazolyl, oxazolyl, isoxazolyl, imidazolyl, pyridyl or pyrimidyl, wherein said thienyl, furyl, pyrrolyl, thiazolyl, oxazolyl, isoxazolyl, imidazolyl, pyridyl and pyrimidyl are optionally substitated with one or more substituents selected from the group consisting of halogen, nitro, C ⁇ -6 alkyl optionally substitated by mono-, di-, or tri-halogen, . 6 alkoxy optionally substitated by mono-, di-, or tri- halogen, and C ⁇ - 6 alkylthio optionally substitated by mono-, di-, or tri- halogen.
  • hydroxy-tefrahydro-naphthalene derivative of formula (I) are those wherein; n represents an integer of 0 or 1 ; and
  • R 1 represents pyridyl or isoxazolyl, wherein said pyridyl and oxazolyl are optionally substitated with one or more substituents selected from the group consisting of halogen, nitro, C_. 6 alkyl optionally substitated by mono-, di-, or tri-halogen, C_ -6 alkoxy optionally substituted by mono-, di-, or tri- halogen, and C 1-6 alkylthio optionally substitated by mono-, di-, or tri- halogen.
  • said hydroxy-tefrahydro-naphthalene derivative of the formula (I) is selected from the group consisting of:
  • This invention is to provide a hydroxy-tefrahydro-naphthalene derivatives of the formula (I), their tautomeric and stereoisomeric form, and salts thereof:
  • R 1 represents aryl or heteroaryl, wherein said aryl and heteroaryl are optionally substitated with one or more substitaents selected from the group consisting of halogen, nitro, hydroxy, carboxy, amino, C_ -6 alkylamino, di(C__ 6 alkyl)amino, C 3 _ 8 cycloalkylamino, C_. 6 alkoxycarbonyl, phenyl (which phenyl is optionally substituted by halogen, trifluoromethyl, trifluoromethoxy, nitro, hydroxy, carboxy, amino, C__ 6 alkylamino, di(Cj . .
  • R 11 represents aryl or heteroaryl, wherein said aryl and heteroaryl are optionally substitated with one or more substitaents selected from the group consisting of halogen, nitro, hydroxy, carboxy, amino, C ⁇ -6 alkylamino, di(C ⁇ _ 6 alkyl)amino, C 3-8 cycloalkylamino, C ⁇ -6 alkoxycarbonyl, phenyl (which phenyl is optionally substituted by halogen, nitro, hydroxy, carboxy, amino, C ⁇ -6 alkylamino, di(C ⁇ -6 alkyl)amino, C 3 _ 8 cycloalkylamino, or C_ -6 alkoxycarbonyl), benzyl (in which phenyl moiety is optionally substitated by halogen, nitro, hydroxy, carboxy, amino, C .
  • R 12 represents aryl, heteroaryl, or C . .6 alkyl optionally substituted by aryl or heteroaryl, wherein said aryl and heteroaryl are optionally substitated with one or more substitaents selected from the group consisting of halogen, nitro, hydroxy, carboxy, amino, C ⁇ _ 6 alkylamino, di(C ⁇ _ 6 alkyl)amino, C 3-8 cycloalkylamino, C_.
  • R 13 represents hydrogen, or C 1-6 alkyl
  • said aryl is optionally substitated with one or more substituents selected from the group consisting of halogen, nitro, hydroxy, carboxy, amino, C ⁇ -6 alkylamino, di(C_. 6 alkyl)amino,
  • hydroxy-tetrahydro-naphthalenylurea derivatives of formula (I) can be those wherein;
  • R 1 represents phenyl, naphthyl, pyridyl, pyrimidyl, indolyl, benzofuranyl, benzo- thiophenyl, quinolinyl or isoquinolinyl, wherein said phenyl, naphthyl, pyridyl, pyrimidyl, indolyl, benzofuranyl, benzothiophenyl, quinolinyl and isoquinolinyl are optionally substitated with one or more substituents selected from the group consisting of halogen, nitro, hydroxy, carboxy, amino, C__ 6 alkylamino, di(C 1-6 alkyl)amino, C 3-8 cycloalkylamino, C ⁇ -6 alkoxycarbonyl, phenyl (which phenyl is optionally substituted by halogen, nitro, hydroxy, carboxy, amino, - ⁇ alkylamino, di(C_.
  • the hydroxy-tetrahydro-naphthalenylurea derivatives of formula (I) can be those wherein; R 1 represents phenyl, pyridyl, or pyrimidyl, wherein said phenyl, pyridyl, and pyrimidyl are optionally substitated by one or more of substitaents selected from the group consisting of halogen, nitro, C_ -6 alkyl (which alkyl is optionally substitated by cyano, nitro, or mono-, di-, or tri-halogen), and C ⁇ -6 alkoxy optionally substitated by mono-, di-, or tri- halogen.
  • R 1 represents phenyl, pyridyl, or pyrimidyl, wherein said phenyl, pyridyl, and pyrimidyl are optionally substitated by one or more of substitaents selected from the group consisting of hal
  • hydroxy-tetrahydro-naphthalenylurea derivatives of formula (I) can be those wherein; represents C 1-6 alkyl optionally substitated by R n , OR 12 , SR 12 or N(R 12 )(R 13 ),
  • R 11 represents phenyl, naphthyl, pyridyl or pyrimidyl, wherein said phenyl, naphthyl, pyridyl and pyrimidyl are optionally substitated with one or more substitaents selected from the group consisting of halogen, nitro, hydroxy, carboxy, amino, C_ -6 alkylamino, di(C ⁇ -6 alkylamino, C 3 _s cycloalkylamino, C ⁇ _ 6 alkoxycarbonyl, benzyl (in which phenyl moiety is optionally substitated by halogen, nitro, hydroxy, carboxy, amino, C ⁇ -6 alkylamino, di(C_ -6 alkyl), amino, C 3 .
  • .6 alkoxy (which alkoxy is optionally substituted by mono-, di-, or tri- halogen), phenoxy (in which phenyl moiety is optionally substituted by halogen, nitro, hydroxy, carboxy, amino, C__6 alkylamino, di(C ⁇ .6 alkyl)amino, C 3 _ 8 cycloalkylamino, or Cj.6 alkoxycarbonyl or C ⁇ _ 6 alkyl), _6 alkylthio (which alkylthio is optionally substituted by mono-, di-, or tri- halogen), C 3-8 cycloalkyl, and heterocycle;
  • R 12 represents pheny, naphthyl, pyridyl, pyrimidyl, or C ⁇ _ 6 alkyl optionally substituted by phenyl, naphthyl, pyridyl or pyrimidyl, wherein said phenyl, naphthyl, pyridyl and pyrimidyl are optionally substitated with one or more substituents selected from the group consisting of halogen, nitro, hydroxy, carboxy, amino, C 1-6 alkylamino, di(C_ -6 alkylamino, C 3-8 cycloalkylamino, C_.6 alkoxycarbonyl, phenyl (which phenyl is optionally substituted by halogen, nitro, hydroxy, carboxy, amino, C_ -6 alkylamino, di(C_ -6 alkyl)amino, C 3-8 cycloalkylamino, or C 1-6 alkoxy- carbonyl), benz
  • hydroxy-tetrahydro-naphthalenylurea derivatives of formula (I) can be those wherein;
  • R 1 represents C_. alkyl optionally substitated by phenyl (which phenyl is optionally substitated with one or more substitaents selected from the group consisting of halogen, nitro, Cj -6 alkyl optionally substitated by cyano, C 1-6 alkoxycarbonyl or mono-, di-, or tri-halogen, and C ⁇ -6 alkoxy optionally substitated by mono-, di-, or tri- halogen), or N(R 12 )(R 13 ),
  • R 12 represents phenyl or C_ -2 alkyl optionally substitated by phenyl, wherein said phenyl is optionally substitated with one or more substitaents selected from the group consisting of halogen, nitro, C ⁇ _ 6 alkyl optionally substituted by mono-, di-, or tri-halogen, and Cj. 6 alkoxy optionally substitated by mono-, di-, or tri- halogen; and
  • R 13 represents hydrogen, or C_. 6 alkyl.
  • hydroxy-tefrahydro-naphthalene derivative of formula (I) are those wherein;
  • R 1 represents C 3 . 8 cycloalkyl optionally fused by phenyl, wherein said phenyl is optionally substitated with one or more substitaents selected from ' the group consisting of halogen, nitro, C ⁇ . 6 alkyl optionally substituted by mono-, di-, or tri-halogen, and C . 6 alkoxy optionally substitated by mono-, di-, or tri- halogen.
  • said hydroxy-tefrahydro-naphthalene derivative of the formula (I) is selected from the group consisting of: N-(7-hydroxy-5,6,7,8-tetrahydronaphthalen-l-yl)-2-methoxybenzamide;
  • This invention is to provide an urea derivatives of the formula (I), their tautomeric and stereoisomeric form, and salts thereof:
  • -X- represents a bond, -O- or -NQR. 1 )- (wherein R 1 is hydrogen or C__ 6 allcyl); with the proviso that when m is 0, -X- represents a bond.
  • Qi, Q 2 and Q 3 independently represent ⁇ or CH, with the proviso that at least one of Q_, Q2 and Q 3 is ⁇ ;
  • R represents aryl or heteroaryl, wherein said aryl and heteroaryl are optionally substitated with one or more substituents independently selected from the group consisting of halogen, nitro, hydroxy, carboxy, amino, C ⁇ -6 alkylamino, di(C ⁇ -6 alkyl)amino, C 3 .
  • urea derivatives of formula (I) can be those wherein;
  • n 0, 1, 2, or 3 ;
  • p 0 or 1
  • -X- represents a bond, -O- or -N ⁇ 1 )- (wherein R 1 is hydrogen or C ⁇ -6 alkyl); with the proviso that when m is 0, -X- represents a bond.
  • Qi, Q 2 and Q 3 independently represent N or CH, with the proviso that at least one of Q_, Q 2 and Q 3 is N;
  • R represents phenyl, naphthyl, pyridyl, or pyrimidyl, wherein said phenyl, naphthyl, pyridyl and pyrimidyl are optionally substituted with one or more substituents independently selected from the group consisting of halogen, nitro, hydroxy, carboxy, amino, .6 alkylamino, di(C 1-6 alkyl)amino, C 3-8 cyclo- alkylamino, C ⁇ -6 alkoxycarbonyl, phenyl (which phenyl is optionally substituted by halogen, nitro, hydroxy, carboxy, amino, C ⁇ -6 alkylamino, di(C ⁇ -6 alkyl)amino, C 3-8 cycloalkylamino, or C ⁇ -6 alkoxycarbonyl), benzyl (in which phenyl moiety is optionally substitated by halogen, nitro, hydroxy, carboxy, amino, C ⁇
  • the urea derivatives of formula (I) can be those wherein; m represents 0, 1, 2, or 3 ; p represents 0 or 1;
  • -X- represents a bond, -O- or -N(R ] )- (wherein R 1 is hydrogen or C ⁇ -6 alkyl); with the proviso that when m is 0, -X- represents a bond.
  • R represents phenyl, naphthyl, pyridyl, or pyrimidyl, wherein said phenyl, naphthyl, pyridyl and pyrimidyl are optionally substitated with one or more substitaents independently selected from the group consisting of halogen, nitro, hydroxy, carboxy, amino, C ⁇ -6 alkylamino, di(C ⁇ -6 alkyl)amino, C 3 .
  • the urea derivatives of formula (I) can be those wherein; m represents 0, 1, 2, or 3; p represents 0 or 1;
  • -X- represents a bond, -O- or -N(R')- (wherein R 1 is hydrogen or C ⁇ -6 allcyl); with the proviso that when m is 0, -X- represents a bond.
  • R represents phenyl, naphthyl, pyridyl, or pyrimidyl, wherein said phenyl, naphthyl, pyridyl and pyrimidyl are optionally substitated with one or more substituents independently selected from the group consisting of halogen, nitro, hydroxy, carboxy, amino, C ⁇ _ 6 alkylamino, di(C ⁇ . 6 alkyl)amino, C 3-8 cycloalkylamino, Ci.6 alkoxycarbonyl, phenyl (which phenyl is optionally substituted by halogen, nitro, hydroxy, carboxy, amino, C ⁇ -6 alkylamino, di(C ⁇ .
  • n 0, 1, 2, or 3 ;
  • p 0 or 1
  • -X- represents a bond, -O- or -N(R')- (wherein R 1 is hydrogen or C ⁇ -6 alkyl); with the proviso that when m is 0, -X- represents a bond.
  • R represents phenyl, naphthyl, pyridyl, or pyrimidyl, wherein said phenyl, naphthyl, pyridyl and pyrimidyl are optionally substitated with one or more substitaents independently selected from the group consisting of halogen, nitro, hydroxy, carboxy, amino, C 1-6 alkylamino, di(C ⁇ -6 alkyl)amino, C 3-8 cycloalkylamino, C ⁇ -6 alkoxycarbonyl, phenyl (which phenyl is optionally substitated by halogen, nitro, hydroxy, carboxy, amino, Cj.
  • the urea derivatives of formula (I) can be those wherein; m represents 0, 1, 2, or 3 ; p represents 0 or 1;
  • -X- represents a bond, -O- or -N ⁇ 1 )- (wherein R 1 is hydrogen or C__ 6 allcyl); with the proviso that when m is 0, -X- represents a bond.
  • Q 2 represents CH;
  • Q 3 represents ⁇ ;
  • R represents phenyl, naphthyl, pyridyl, or pyrimidyl, wherein said phenyl, naphthyl, pyridyl and pyrimidyl are optionally substituted with one or more substituents independently selected from the group consisting of halogen, nitro, hydroxy, carboxy, amino, Cj.6 alkylamino, di(C_.
  • urea derivative of formula (I) are those wherein; m represents 0; p represents 0 or 1;
  • Q_, Q 2 and Q 3 independently represent N or CH, with the proviso that at least one of Q b Q 2 and Q 3 is N;
  • R represents phenyl, naphthyl, pyridyl, or pyrimidyl, wherein said phenyl, naphthyl, pyridyl and pyrimidyl are optionally substituted with one or more substitaents independently selected from the group consisting of halogen, nitro, hydroxy, carboxy, amino, C 1-6 alkylamino, di(C ⁇ .6 alkyl)amino, C 3-8 cycloalkylamino, .
  • the urea derivative of formula (T) are those wherein; m represents 0, 1, 2, or 3 ; p represents 0 or 1;
  • -X- represents a bond, -O- or -N(R')- (wherein R 1 is hydrogen or C ⁇ _ 6 alkyl); with the proviso that when m is 0, -X- represents a bond.
  • Qi, Q 2 and Q 3 independently represent N or CH, with the proviso that at least one of Qi, Q 2 and Q 3 is N;
  • R represents phenyl, naphthyl, pyridyl, or pyrimidyl, wherein said phenyl, naphthyl, pyridyl, or pyrimidyl is optionally substitated by one or more of substituents selected from the group consisting of chloro, bromo, fluoro, nitro, methoxy, trifluoromethyl, trifluoromethoxy and C_. ⁇ alkanoylamino.
  • said urea derivative of the formula (I) is selected from the group consisting of:
  • the compounds of the present invention are, therefore suitable especially for the prophylaxis and freatment of diseases associated with VRl activity, in particular for the treatment of .
  • urological diseases or disorders such as detrusor overactivity (overactive bladder), urinary incontinence, neurogenic detrusor oeractivity (detrusor hyperflexia), idiopathic detrusor overactivity (detrusor instability), benign prostatic hype ⁇ lasia, and lower urinary tract symptoms.
  • the compounds of the present invention are also effective for treating or preventing a disease selected from the group consisting of chronic pain, neuropathic pain, postoperative pain, rheumatoid arthritic pain, neuralgia, neuropathies, algesia, nerve injury, ischaemia, neuro- degeneration and/or stroke, as well as inflammatory diseases such as asthma and COPD since the diseases also relate to VRl activity.
  • a disease selected from the group consisting of chronic pain, neuropathic pain, postoperative pain, rheumatoid arthritic pain, neuralgia, neuropathies, algesia, nerve injury, ischaemia, neuro- degeneration and/or stroke, as well as inflammatory diseases such as asthma and COPD since the diseases also relate to VRl activity.
  • the compounds of the present invention are also useful for the treatment and prophylaxis of neuropathic pain, which is a form of pain often associated with he ⁇ es zoster and post-he ⁇ etic neuralgia, painful diabetic neuropathy, neuropathic low back pain, postfraumatic and postoperative neuralgia, neuralgia due to nerve compression and other neuralgias, phantom pain, complex regional pain syndromes, infectious or parainfectious neuropathies like those associated with HTV infection, pain associated with central nervous system disorders like multiple sclerosis or Parkinson disease or spinal cord injury or traumatic brain injury, and post-stroke pain.
  • neuropathic pain which is a form of pain often associated with he ⁇ es zoster and post-he ⁇ etic neuralgia, painful diabetic neuropathy, neuropathic low back pain, postfraumatic and postoperative neuralgia, neuralgia due to nerve compression and other neuralgias, phantom pain, complex regional pain syndromes, infectious or parainfectious neuropath
  • the compounds of the present invention are useful for the freatment of musculoskeletal pain, forms of pain often associated with osteoarthritis or rheumatoid arthritis or other forms of arthritis, and back pain.
  • the compounds of the present invention are useful for the treatment of pain associated with cancer, including visceral or neuropathic pain associated with cancer or cancer treatment.
  • the compounds of the present invention are furthermore useful for the treatment of visceral pain, e.g. pain associated with obstruction of hollow viscus like gallstone colik, pain associated with irritable bowel syndrome, pelvic pain, vulvodynia, orchialgia or prostatodynia, pain associated with inflammatory lesions of joints, skin, muscles or nerves, and orofascial pain and headache, e.g. migraine or tension-type headache.
  • visceral pain e.g. pain associated with obstruction of hollow viscus like gallstone colik
  • pain associated with irritable bowel syndrome pelvic pain
  • vulvodynia orchialgia or prostatodynia
  • pain associated with inflammatory lesions of joints, skin, muscles or nerves e.g. migraine or tension-type headache.
  • the present invention provides a medicament, which includes one of the compounds, described above and optionally pharmaceutically acceptable excipients.
  • Alkyl per se and "alk” and "alkyl” in alkenyl, alkynyl, alkoxy, alkanoyl, alkylamino, alkylamino- carbonyl, alkylaminosulfonyl, alkylsulfonylamino, alkoxycarbonyl, alkoxycarbonylamino and alkanoylamino represent a linear or branched alkyl radical having generally 1 to 6, preferably 1 to 4 and particularly preferably 1 to 3 carbon atoms, representing illustratively and preferably methyl, ethyl, n-propyl, isopropyl, tert-butyl, n-pentyl and n-hexyl.
  • Alkoxy illustratively and preferably represents methoxy, ethoxy, n-propoxy, isopropoxy, tert- butoxy, n-pentoxy and n-hexoxy.
  • Alkylamino illustratively and preferably represents an alkylamino radical having one or two (independently selected) allcyl substituents, illustratively and preferably representing methylamino, ethylamino, n-propylamino, isopropylamino, tert-butylamino, n-pentylamino, n-hexylamino, N,N- dimethylamino, N ⁇ -diethylamino, ⁇ -ethyl- ⁇ -methylamino, N-methyl- ⁇ -n-propylamino, N- isopropyl- ⁇ -n-propylamino, ⁇ -t-butyl-N-methylamino, N-ethyl-N-n-pentylamino and N-n-hexyl- ⁇ -methylamino.
  • Aryl per se and in arylamino and in arylcarbonyl represents a mono- to tricyclic aromatic carbocyclic radical having generally 6 to 14 carbon atoms, illustratively and preferably representing phenyl, naphthyl and phenanthrenyl.
  • Cycloalkyl per se and in cycloalkylamino and in cycloalkylcarbonyl represents a cycloalkyl group having generally 3 to 8 and preferably 5 to 7 carbon atoms, illustratively and preferably representing cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl and cycloheptyl.
  • Heteroaryl per se and the heteroaryl portion of the heteroaralkyl, heteroaryloxy, heteroaralkyloxy, or heteroarylcarbamoyl represent an aromatic mono- or bicyclic radical having generally 5 to 10 and preferably 5 or 6 ring atoms and up to 5 and preferably up to 4 hetero atoms selected from the group consisting of S, O and N, illustratively and preferably representing thienyl, furyl, pynolyl, thiazolyl, oxazolyl, imidazolyl, pyridyl, pyrimidyl, pyridazinyl, indolyl, isoindolino, indazolyl, benzofuranyl, benzothiophenyl, quinolinyl, isoquinolinyl, tetrazolyl, and triazolyl.
  • Heterocyclyl per se and in heterocyclylcarbonyl represents a mono- or polycyclic, preferably mono- or bicyclic, nonaromatic heterocyclic radical having generally 4 to 10 and preferably 5 to 8 ring atoms and up to 3 and preferably up to 2 hetero atoms and/or hetero groups selected from the group consisting of ⁇ , O, S, SO and S0 2 .
  • the heterocyclyl radicals can be saturated or partially unsatarated.
  • CHAPTER I EMBODIMENT OF THE INVENTION
  • the compound of the formula (I) of the present invention can be, but not limited to be, prepared by combining various known methods.
  • one or more of the substitaents, such as amino group, carboxyl group, and hydroxyl group of the compounds used as starting materials or intermediates are advantageously protected by a protecting group known to those skilled in the art. Examples of the protecting groups are described in "Protective Groups in Organic Synthesis (3rd Edition)" by Greene and Wuts, John Wiley and Sons, New York 1999.
  • the compound of the formula (T) of the present invention can be, but not limited to be, prepared by the Method [A], [B], [C], [D], [E] or [F] below.
  • the compound of the formula (I) (wherein n, Q_, Q , Q 3 , Q and R 4 are the same as defined above) can be prepared by the reaction of the compound of the formula (II) (wherein Qj, Q 2 , Q 3 and Q are the same as defined above) and the compound of the formula (HI) (wherein n and R 4 are the same as defined above).
  • the reaction may be carried out in a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane; ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; aromatic hydrocarbons such as benzene, toluene and xylene; nitriles such as acetonitrile; amides such as N, N- dimethylformamide (DMF), ⁇ , ⁇ -dimethylacetamide (DMAC) and ⁇ -methylpynolidone (NMP); urea such as l,3-dimethyl-2-imidazolidinone (DMI); sulfoxides such as dimethylsulfoxide (DMSO); and others.
  • a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-d
  • the reaction can be carried out in the presence of organic base such as pyridine or triethylamine.
  • the reaction temperature can be optionally set depending on the compounds to be reacted.
  • the reaction temperatare is usually, but not limited to, about room temperatare to 100°C.
  • the reaction may be conducted for, usually, 30 minutes to 48 hours and preferably 1 to 24 hours.
  • the compound (II) and (HI) can be prepared by the use of known techniques or are commercially available.
  • the compound of the formula (I) (wherein n, Qi, Q 2 , Q 3 , Q 4 and R are the same as defined above) can be prepared by reacting the compound of the formula (IT) (wherein Q_, Q 2 , Q 3 and Q 4 are the same as defined above) with phosgene, diphosgene, triphosgene, 1,1-carbonyldiimidazole (CDI), or l,r-carbonyldi(l,2,4-triazole)(CDT), and then adding the compound of the formula (IV) (wherein n and R 4 are the same as defined above) to the reaction mixture.
  • the reaction may be carried out in a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane; ethers such as diethyl ether, iso- propyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; aromatic hydrocarbons such as benzene, toluene and xylene; nitriles such as acetonitrile; amides such as N, N- dimethylformamide (DMF), N, N-dimefhylacetamide (DMAC) and N-methylpynolidone (NMP); urea such as l,3-dimethyl-2-imidazolidinone (DMI); sulfoxides such as dimethylsulfoxide (DMSO); and others.
  • a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-d
  • the reaction temperatare can be optionally set depending on the compounds to be reacted.
  • the reaction temperatare is usually, but not limited to, about 20°C to 50°C.
  • the reaction may be conducted for, usually, 30 minutes to 10 hours and preferably 1 to 24 hours.
  • the compound (IV) is commercially available or can be prepared by the use of known techniques and phosgene, diphosgene, triphosgene, CDI, and CDT are commercially available and . [Method C]
  • the compound of the formula (I) (wherein n, Q ls Q 2 , Q 3 , Q and R 4 are the same as defined above) can be prepared by reacting the compound of the formula (IT) (wherein Q., Q 2 , Q 3 and Q 4 are the same as defined above) with the compound of the formula (V) (wherein L_ represents halogen atom such as chlorine, bromine, or iodine atom) and then adding the compound of the formula (IV) (wherein n and R 4 are the same as defined above) to the reaction mixture.
  • the reaction may be carried out in a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane; ethers such as diethyl ether, iso- propyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; aromatic hydrocarbons such as benzene, toluene and xylene; nitriles such as acetonifrile; amides such as N, N- dimethylformamide (DMF), N, N-dimethylacetamide (DMAC) and N-methylpynolidone (NMP); urea such as l,3-dimethyl-2-imidazolidinone (DMI); sulfoxides such as dimethylsulfoxide (DMSO); and others.
  • a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichlor
  • the reaction temperature can be optionally set depending on the compounds to be reacted.
  • the reaction temperature is usually, but not limited to, about 20°C to 50°C.
  • the reaction may be conducted for, usually, 30 minutes to 10 hours and preferably 1 to 24 hours.
  • the reaction can be advantageously carried out in the presence of a base including, for instance, organic amines such as pyridine, friethylamine and N,N-diisopropylethylamine, dimethylaniline, diethylaniline, 4-dimethylaminopyridine, and others.
  • a base including, for instance, organic amines such as pyridine, friethylamine and N,N-diisopropylethylamine, dimethylaniline, diethylaniline, 4-dimethylaminopyridine, and others.
  • the compound of the formula (I) (wherein n, Q Q 2 , Q 3 , Q and R 4 are the same as defined above) can be prepared by reacting the compound of the formula (IV) (wherein n and R 4 are the same as defined above) with phosgene, diphosgene, triphosgene, 1,1-carbonyldiimidazole (CDI), or 1,1'- carbonyldi(l,2,4-triazole)(CDT) and then adding the compound of the formula (B) (wherein Q_, Q 2 , Q 3 and Q 4 are the same as defined above) to the reaction mixture.
  • the reaction may be carried out in a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane; ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; aromatic hydrocarbons such as benzene, toluene and xylene; nitriles such as acetonitrile; amides such as N, N- dimethylformamide (DMF), N, N-dimethylacetamide (DMAC) and N-methylpynolidone (NMP); urea such as l,3-dimethyl-2-imidazolidinone (DMI); sulfoxides such as dimethylsulfoxide (DMSO); and others.
  • a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloro
  • the reaction temperature can be optionally set depending on the compounds to be reacted.
  • the reaction temperatare is usually, but not limited to, about 20°C to 50°C.
  • the reaction may be conducted for, usually, 30 minutes to 10 hours and preferably 1 to 24 hours.
  • the compound of the formula (I) (wherein n, Qi, Q 2 , Q 3 , Q 4 and R 4 are the same as defined above) can be prepared by reacting the compound of the formula (TV) (wherein n and R 4 are the same as defined above) with the compound of the formula (V) (wherein Lj is the same as defined above) and then adding the compound of the formula (TT) (wherein Q ls Q 2 , Q 3 and Q are the same as defined above) to the reaction mixture.
  • Qi, Q 2 , Q 3 and Q 4 and R 4 can be prepared by reacting the compound of the formula (TV) (wherein n and R 4 are the same as defined above) with the compound of the formula (V) (wherein Lj is the same as defined above) and then adding the compound of the formula (TT) (wherein Q ls Q 2 , Q 3 and Q are the same as defined above) to the reaction mixture.
  • the reaction may be carried out in a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane; ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; aromatic hydrocarbons such as benzene, toluene and xylene; nitriles such as acetonitrile; amides such as N, N- dimethylformamide (DMF), N, N-dimethylacetamide (DMAC) and N-methylpynolidone (NMP); urea such as l,3-dimethyl-2-imidazolidinone (DMT); sulfoxides such as dimethylsulfoxide (DMSO); and others.
  • a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloro
  • the reaction temperature can be optionally set depending on the compounds to be reacted.
  • the reaction temperatare is usually, but not limited to, about 20°C to 50°C.
  • the reaction may be conducted for, usually, 30 minutes to 10 hours and preferably 1 to 24 hours.
  • the reaction can be advantageously canied out in the presence of a base including, for instance, organic amines such as pyridine, friethylamine and NN-diisopropylethylamine, dimethylaniline, diethylaniline, 4-dimethylaminopyridine, and others.
  • a base including, for instance, organic amines such as pyridine, friethylamine and NN-diisopropylethylamine, dimethylaniline, diethylaniline, 4-dimethylaminopyridine, and others.
  • the compound of the formula (I-a) (wherein n and R 4 are the same as defined above) can be prepared in the similar manner as described in Method [A], [B], [C], [D] or [E] for the preparation of the compound of the formula (I) by using a compound of the formula (VT) instead of the compound of the formula (IT).
  • the compound of the formula (I-b) (wherein n and R 4 are the same as defined above) can be prepared by reacting the compound of the formula (I-a) (wherein n and R 4 are the same as defined above) with an acid such as hydrochloric acid.
  • the reaction may be carried out in a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane; ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; alcohols such as methanol, ethanol; water and others.
  • halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane
  • ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane
  • alcohols such as methanol, ethanol; water and others.
  • two or more of the solvents selected from the listed above can be mixed and used.
  • the reaction temperature can be optionally set depending on the compounds to be reacted.
  • the reaction temperature is usually, but not limited to, about 20°C to 100°C.
  • the reaction may be conducted for, usually, 30 minutes to 10 hours and preferably 1 to 24 hours.
  • the compound of the formula (I-c) (wherein n and R 4 are the same as defined above) can be prepared by reacting the compound of the formula (I-b) (wherein n and R 4 are the same as defined above) with reducing agent such as sodium borohydride or lithium aluminum hydride.
  • the reaction may be carried out in a solvent including, for instance, ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; alcohols such as methanol, ethanol, isopropanol, and others.
  • ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane
  • alcohols such as methanol, ethanol, isopropanol, and others.
  • two or more of the solvents selected from the listed above can be mixed and used.
  • the reaction temperature can be optionally set depending on the compounds to be reacted.
  • the reaction temperature is usually, but not limited to, about 20°C to 50°C.
  • the reaction may be conducted for, usually, 30 minutes to 10 hours and preferably 1 to 24 hours.
  • the compound of the formula (I) of the present invention can be, but not limited to be, prepared by combining various known methods.
  • one or more of the substitaents, such as amino group, carboxyl group, and hydroxyl group of the compounds used as starting materials or intermediates are advantageously protected by a protecting group known to those skilled in the art. Examples of the protecting groups are described in "Protective Groups in Organic Synthesis (3rd Edition)" by Greene and Wuts, John Wiley and Sons, New York 1999.
  • the compound of the formula (I) of the present invention can be, but not limited to be, prepared by the Method [A] below.
  • the compound of the formula (I) of the present invention can be, but not limited to be, prepared by the Method [A], [B], [C], [D], [E], [F], [G] or [H] below.
  • the compound of the formula (I) (wherein n and R 1 are the same as defined above) can be prepared by reacting the compound of the formula (II) and the compound of the formula (DI) (wherein Lj represents halogen atom such as chlorine, bromine, or iodine atom) and then adding the compound of the formula (TV) (wherein n, R 1 are the same as defined above) to the reaction mixtare.
  • the reaction may be carried out in a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane; ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; aromatic hydrocarbons such as benzene, toluene and xylene; nitriles such as acetonitrile; amides such as N, N- dimethylformamide (DMF), N, N-dimethylacetamide (DMAC) and N-methylpynolidone (NMP); urea such as l,3-dimethyl-2-imidazolidinone (DMI); sulfoxides such as dimethylsulfoxide (DMSO); and others.
  • halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane
  • ethers
  • reaction temperature can be optionally set depending on the compounds to be reacted.
  • the reaction temperatare is usually, but not limited to, about 20°C to 50°C.
  • the reaction may be conducted for, usually, 30 minutes to 10 hours and preferably 1 to 24 hours.
  • the reaction can be advantageously carried out in the presence of a base including, for instance, organic amines such as pyridine, triethylamine and NN-diisopropylethylamine, dimethylaniline, diethylaniline, 4-dimethylaminopyridine, and others.
  • a base including, for instance, organic amines such as pyridine, triethylamine and NN-diisopropylethylamine, dimethylaniline, diethylaniline, 4-dimethylaminopyridine, and others.
  • the compound (DI) and (IV) are commercially available or can be prepared by the use of known techniques.
  • the compound of the formula (I) (wherein n and R 1 are the same as defined above) can be prepared by the reaction of the compound of the formula (II) and the compound of the formula (V) (wherein n and R 1 are the same as defined above).
  • the reaction may be carried out in a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane; ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; aromatic hydrocarbons such as benzene, toluene and xylene; nitriles such as acetonitrile; amides such as N, N- dimethylformamide (DMF), N, N-dimethylacetamide (DMAC) and N-methylpynolidone (NMP); urea such as l,3-dimethyl-2-imidazolidinone (DMI); sulfoxides such as dimethylsulfoxide (DMSO); and others.
  • a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloro
  • the reaction can be carried out in the presence of organic base such as pyridine or friethylamine.
  • the reaction temperatare can be optionally set depending on the compounds to be reacted.
  • the reaction temperatare is usually, but not limited to, about room temperature to 100°C.
  • the reaction may be conducted for, usually, 30 minutes to 48 hours and preferably 1 to 24 hours.
  • the compound (V) can be prepared by the use of known techniques or are commercially available. [Method C]
  • the compound of the formula (I) (wherein n and R 1 are the same as defined above) can be prepared by reacting the compound of the formula (TT) with phosgene, diphosgene, triphosgene, 1,1-carbonyldiimidazole (CDI), or l, -carbonyldi(l,2,4-triazole)(CDT), and then adding the compound of the formula (IV) (wherein n and R 1 are the same as defined above) to the reaction mixture.
  • the reaction may be ca ied out in a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane; ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; aromatic hydrocarbons such as benzene, toluene and xylene; nitriles such as acetonitrile; amides such as N, N- dimethylformamide (DMF), N, N-dimethylacetamide (DMAC) and N-methylpynolidone (NMP); urea such as l,3-dimethyl-2-imidazolidinone (DMI); sulfoxides such as dimethylsulfoxide (DMSO); and others.
  • halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane
  • the reaction temperature can be optionally set depending on the compounds to be reacted.
  • the reaction temperatare is usually, but not limited to, about 20°C to 50°C.
  • the reaction may be conducted for, usually, 30 minutes to 10 hours and preferably 1 to 24 hours.
  • Phosgene, diphosgene, triphosgene, CDI, and CDT are commercially available.
  • the compound of the formula (I) (wherein n and R 1 are the same as defined above) can be prepared by reacting the compound of the formula (IV) (wherein n and R 1 are the same as defined above) with phosgene, diphosgene, triphosgene, 1,1-carbonyldiimidazole (CDI), or 1,1'- carbonyldi(l,2,4-triazole)(CDT) and then adding the compound of the formula (IT) (wherein R 1 is the same as defined above) to the reaction mixture.
  • the reaction may be carried out in a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane; ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; aromatic hydrocarbons such as benzene, toluene and xylene; nitriles such as acetonitrile; amides such as N, N- dimethylformamide (DMF), N, ⁇ -dimethylacetamide (DMAC) and ⁇ -methylpynolidone ( ⁇ MP); urea such as l,3-dimethyl-2-imidazolidinone (DMI); sulfoxides such as dimethylsulfoxide (DMSO); and others.
  • a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dich
  • the reaction temperature can be optionally set depending on the compounds to be reacted.
  • the reaction temperature is usually, but not limited to, about 20°C to 50°C.
  • the reaction may be conducted for, usually, 30 minutes to 10 hours and preferably 1 to 24 hours.
  • the compound of the formula (I) (wherein n and R 1 are the same as defined above) can be prepared by reacting the compound of the formula (IV) (wherein n and R 1 are the same as defined above) and the compound of the formula (ITT) (wherein , ⁇ is the same as defined above), and then adding the compound of the formula (II) to the reaction mixture.
  • the reaction may be carried out in a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane; ethers such as diethyl ether, iso- propyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; aromatic hydrocarbons such as benzene, toluene and xylene; nitriles such as acetonitrile; amides such as N, N- dimethylformamide (DMF), N, N-dimethylacetamide (DMAC) and N-methylpynolidone (NMP); urea such as l,3-dimethyl-2-imidazolidinone (DMI); sulfoxides such as dimethylsulfoxide (DMSO); and others.
  • a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichlor
  • the reaction temperature can be optionally set depending on the compounds to be reacted.
  • the reaction temperatare is usually, but not limited to, about 20°C to 50 °C.
  • the reaction may be conducted for, usually, 30 minutes to 10 hours and preferably 1 to 24 hours.
  • the reaction can be advantageously carried out in the presence of a base including, for instance, organic amines such as pyridine, friethylamine and N,N-diisopropylethylamine, dimethylaniline, diethylaniline, 4-dimethylaminopyridine, and others.
  • a base including, for instance, organic amines such as pyridine, friethylamine and N,N-diisopropylethylamine, dimethylaniline, diethylaniline, 4-dimethylaminopyridine, and others.
  • the compound of the formula (VET) (wherein n and R 1 are the same as defined above) can be prepared by reacting the compound of the formula (VI) with the compound of the formula (V) (wherein n and R 1 are the same as defined above) in a similar manner described in Method B for the preparation of the compound of the formula (I).
  • the compound of the formula (VIII) (wherein n and R 1 are the same as defined above) can be prepared by reacting the compound of the formula (VET) (wherein n and R 1 are the same as defined above) with an acid such as hydrochloric acid.
  • the reaction may be carried out in a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane; ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; alcohols such as methanol, ethanol; water and others.
  • halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane
  • ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane
  • alcohols such as methanol, ethanol; water and others.
  • two or more of the solvents selected from the listed above can be mixed and used.
  • the reaction temperatare can be optionally set depending on the compounds to be reacted.
  • the reaction temperatare is usually, but not limited to, about 20°C to 100°C.
  • the reaction may be conducted for, usually, 30 minutes to 10 hours and preferably 1 to 24 hours.
  • the compound of the formula (I) (wherein n and R 1 are the same as defined above) can be prepared by reacting the compound of the formula (VDI) (wherein n and R 1 are the same as defined above) with reducing agent such as sodium borohydride or lithium aluminum hydride.
  • the reaction may be carried out in a solvent including, for instance, ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; alcohols such as methanol, ethanol, isopropanol, and others.
  • ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane
  • alcohols such as methanol, ethanol, isopropanol, and others.
  • two or more of the solvents selected from the listed above can be mixed and used.
  • the reaction temperatare can be optionally set depending on the compounds to be reacted.
  • the reaction temperatare is usually, but not limited to, about 20°C to 50°C.
  • the reaction may be conducted for, usually, 30 minutes to 10 hours and preferably 1 to 24 hours.
  • the compound (VI) is commercially available or can be prepared by the use of known techniques.
  • the stereoisomeric form of the compound (I), R form (I-a) (wherein n and R 1 are the same as defined above) can be prepared in the similar manner as described in Method [A], [B], [C], [D], or [E] for the preparation of the compound of the formula (I) by using a compound of the formula (II- a) instead of the compound of the formula (II).
  • stereoisomeric form of the compound (I), S form (I-a') (wherein n and R 1 are the same as defined above) can be prepared in the similar manner as described in Method [A], [B], [C], [D], or [E] for the preparation of the compound of the formula (I) by using a compound of the formula (II- a') instead of the compound of the formula (IT).
  • the compound (U-a) or (ll-a') can be prepared by the use of known techniques.
  • the compound of the formula (T) of the present invention can be, but not limited to be, prepared by combining various known methods.
  • one or more of the substituents, such as amino group, carboxyl group, and hydroxyl group of the compounds used as starting materials or intermediates are advantageously protected by a protecting group known to those skilled in the art. Examples of the protecting groups are described in "Protective Groups in Organic Synthesis (3rd Edition)" by Greene and Wuts, John Wiley and Sons, New York 1999.
  • the compound of the formula (I) of the present invention can be, but not limited to be, prepared by the Method [A] below. [Method A] o
  • the compound of the formula (1) (wherein R 1 is the same as defined above) can be prepared by the reaction of the compound of the formula (IT) with the compound of the formula (HI) (wherein R 1 is the same as defined above and Li represents a leaving group including, for instance, hydroxy, halogen atom such as chlorine, bromine, or iodine atom, or azole such as imidazole or triazole.).
  • the reaction may be carried out in a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane; ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; aromatic hydro- carbons such as benzene, toluene and xylene; nitriles such as acetonitrile; amides such as N,N- dimethylformamide (DMF), N,N-dimethylacetamide (DMAC) and N-methylpynolidone (NMP); ureas such as l,3-dimethyl-2-imidazolidinone (DMI); sulfoxides such as dimethylsulfoxide (DMSO); and others.
  • a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dich
  • the reaction temperatare can be optionally set depending on the compounds to be reacted.
  • the reaction temperatare is usually, but not limited to, about 0°C to 50°C.
  • the reaction may be conducted for, usually, 30 minutes to 24 hours and preferably 1 to 10 hours.
  • the reaction can be advantageously carried out in the presence of a base including, for instance, organic amines such as pyridine, friethylamine and N,N-diisopropylethylamine, dimethylaniline, diethylaniline, 4-dimethylaminopyridine, and others.
  • a base including, for instance, organic amines such as pyridine, friethylamine and N,N-diisopropylethylamine, dimethylaniline, diethylaniline, 4-dimethylaminopyridine, and others.
  • the reaction can be advantageously carried out using coupling agent including, for instance, hydroxybenzotriazole, carbodiimides such as N, N-dicyclohexylcarbodi- imide and l-(3-dimethyl-aminopropyl)-3-ethylcarbodiimide; carbonyldiazoles such as 1,1'- carbonyldi(l,3-imiazole)(CDI) and l,l'-carbonyldi(l,2,4-triazole)(CDT), and the like.
  • the compound (B) and (IH) are commercially available or can be prepared by the use of known techniques.
  • the compound of the formula (I-a) (wherein n is 1 to 6; and Xj is OR 12 , SR 12 or N(R 12 )(R 13 ) (in which R 12 and R 13 are the same as defined above)) can be, but not limited to be, prepared by the following procedures.
  • Step B-1 the compound of the formula (V) (wherein n is 1 to 6; L represents a leaving group including, for instance, hydroxy, halogen atom such as chlorine, bromine, or iodine atom, or azole such as imidazole or triazole; and L 2 represents a leaving group including, for instance, halogen atom such as chlorine, bromine, or iodine atom) can be prepared in a similar manner as described in Method [A] by using a compound of the formula (IV) (wherein n, L ⁇ and L 2 are the same as defined above) instead of the compound of the formula (ITT).
  • Step B-2 the compound of the formula (I-a) (wherein n and X_ are the same as defined above) can be, but not limited to be, prepared by the reaction of the compound of the formula (V) (wherein n and L 2 are the same as defined above) with the compound of the formula (VI) (wherein Xi is the same as defined above).
  • the reaction may be carried out in a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane; ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; aromatic hydrocarbons such as benzene, toluene and xylene; nitriles such as acetonitrile; amides such as N,N- dimethylformamide (DMF), NN-dimethylacetamide (DMAC) and N-methylpynolidone (NMP); ureas such as l,3-dimethyl-2-imidazolidinone (DMI); sulfoxides such as dimethylsulfoxide (DMSO); and others.
  • halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane
  • ethers
  • reaction temperature can be optionally set depending on the compounds to be reacted.
  • the reaction temperatare is usually, but not limited to, about 0°C to 50°C.
  • the reaction may be conducted for, usually, 30 minutes to 24 hours and preferably 1 to 10 hours.
  • the reaction can be advantageously canied out in the presence of a base including, for instance, organic amines such as pyridine, triethylamine and NN-diisopropylethylamine, dimethylaniline, diethylaniline, 4-dimethylaminopyridine, and others.
  • a base including, for instance, organic amines such as pyridine, triethylamine and NN-diisopropylethylamine, dimethylaniline, diethylaniline, 4-dimethylaminopyridine, and others.
  • the compound (IV) and (Vf) are commercially available or can be prepared by the use of known techniques.
  • the compound of the formula (I) of the present invention can be, but not limited to be, prepared by combining various known methods.
  • one or more of the substitaents, such as amino group, carboxyl group, and hydroxyl group of the compounds used as starting materials or intermediates are advantageously protected by a protecting group known to those skilled in the art. Examples of the protecting groups are described in "Protective Groups in Organic Synthesis (3rd Edition)" by Greene and Wuts, John Wiley and Sons, New York 1999.
  • the compound of the formula (I) of the present invention can be, but not limited to be, prepared by the Method [A], [B], [C], [D], or [E] below.
  • the compound of the formula (I) (wherein m, p, Q b Q 2 , Q 3 , R and X are the same as defined above) can be prepared by reacting the compound of the formula ( T) (wherein Q Q 2 and Q 3 are the same as defined above) and the compound of the formula (DI) (wherein Li represents a leaving group including halogen atom such as chlorine, bromine, or iodine atom) and then adding the compound of the formula (TV) (wherein m, p, R and X are the same as defined above) to the reaction mixture.
  • the reaction may be carried out in a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane; ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; aromatic hydrocarbons such as benzene, toluene and xylene; nitriles such as acetonitrile; amides such as N, N-dimethylformamide (DMF), N, N-dimethylacetamide (DMAC) and ⁇ -methylpyrrolidone ( ⁇ MP); urea such as l,3-dimethyl-2-imidazolidinone (DMI); sulfoxides such as dimethylsulfoxide (DMSO); and others.
  • a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichlor
  • the reaction temperatare can be optionally set depending on the compounds to be reacted.
  • the reaction temperatare is usually, but not limited to, about 20°C to 50 °C.
  • the reaction may be conducted for, usually, 30 minutes to 24 hours and preferably 1 to 10 hours.
  • the reaction can be advantageously canied out in the presence of a base including, for instance, organic amines such as pyridine, triethylamine and N ⁇ -diisopropylethylamine, dimethylaniline, diethylaniline, 4-dimethylaminopyridine, and others.
  • a base including, for instance, organic amines such as pyridine, triethylamine and N ⁇ -diisopropylethylamine, dimethylaniline, diethylaniline, 4-dimethylaminopyridine, and others.
  • the compound of the formula (DI) and (IV) are commercially available or can be prepared by the use of known techniques.
  • the compound of the formula (I) (wherein m, p, Qi, Q 2 , Q 3 , R and X are the same as defined above) can be prepared by the reaction of the compound of the formula (II) (wherein Q_, Q 2 and Q 3 are the same as defined above) and the compound of the formula (V) (wherein m, p, R and X are the same as defined above).
  • the reaction may be carried out in a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane; ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; aromatic hydrocarbons such as benzene, toluene and xylene; nitriles such as acetonitrile; amides such as N, N- dimethylformamide (DMF), N, N-dimethylacetamide (DMAC) and ⁇ -methylpynolidone ( ⁇ MP); urea such as l,3-dimethyl-2-imidazolidinone (DMT); sulfoxides such as dimethylsulfoxide (DMSO); and others.
  • a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichlor
  • the reaction can be carried out in the presence of organic base such as pyridine or triethylamine.
  • the reaction temperatare can be optionally set depending on the compounds to be reacted.
  • the reaction temperatare is usually, but not limited to, about room temperature to 100°C.
  • the reaction may be conducted for, usually, 30 minutes to 24 hours and preferably 1 to 10 hours.
  • the compound (V) can be prepared by the use of known techniques or are commercially available.
  • the compound of the formula (I) (wherein m, p, Qj, Q 2 , Q 3 , R and X are the same as defined above) can be prepared by reacting the compound of the formula (IT) (wherein Qi, Q 2 and Q 3 are the same as defined above) with phosgene, diphosgene, triphosgene, 1,1-carbonyldiimidazole (CDI), or l,r-carbonyldi(l,2,4-triazole)(CDT), and then adding the compound of the formula (IV) (wherein m, p, R and X are the same as defined above) to the reaction mixtare.
  • the reaction may be canied out in a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane; ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; aromatic hydrocarbons such as benzene, toluene and xylene; nitriles such as acetonitrile; amides such as N, N- dimethylformamide (DMF), N, N-dimethylacetamide (DMAC) and N-methylpynolidone (NMP); urea such as l,3-dimethyl-2-imidazolidinone (DMI); sulfoxides such as dimethylsulfoxide (DMSO); and others.
  • a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichlor
  • the reaction temperatare can be optionally set depending on the compounds to be reacted.
  • the reaction temperature is usually, but not limited to, about 20°C to 50°C.
  • the reaction may be conducted for, usually, 30 minutes to 24 hours and preferably 1 to 10 hours.
  • the compound of the formula (I) (wherein m, p, Qi, Q 2 , Q 3 , R and X are the same as defined above) can be prepared by reacting the compound of the formula (IV) (wherein m, p, R and X are the same as defined above) with phosgene, diphosgene, triphosgene, 1,1-carbonyldiimidazole (CDI), or l, -carbonyldi(l,2,4-triazole)(CDT) and then adding the compound of the formula (TT) (wherein Qj, Q 2 and Q 3 are the same as defined above) to the reaction mixtare.
  • the reaction may be carried out in a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane; ethers such as diethyl ether, isopropyl ether, dioxane and tefrahydrofuran (THF) and 1,2-dimethoxyethane; aromatic hydrocarbons such as benzene, toluene and xylene; nitriles such as acetonitrile; amides such as N, N- dimethylformamide (DMF), N, N-dimethylacetamide (DMAC) and N-methylpynolidone (NMP); urea such as l,3-dimethyl-2-imidazolidinone (DMI); sulfoxides such as dimethylsulfoxide (DMSO); and others.
  • a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dich
  • the reaction temperatare can be optionally set depending on the compounds to be reacted.
  • the reaction temperatare is usually, but not limited to, about 20°C to 50°C.
  • the reaction may be conducted for, usually, 30 minutes to 24 hours and preferably 1 to 10 hours.
  • the compound of the formula (I) (wherein m, p, Qi, Q 2 , Q 3 , R and X are the same as defined above) can be prepared by reacting the compound of the formula (TV) (wherein m, p, R and X are the same as defined above) and the compound of the formula (DT) (wherein Li is the same as defined above), and then adding the compound of the formula (D) (wherein Q Q 2 and Q 3 are the same as defined above) to the reaction mixture.
  • the reaction may be carried out in a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane; ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; aromatic hydrocarbons such as benzene, toluene and xylene; nitriles such as acetonitrile; amides such as N, N- dimethylformamide (DMF), N, N-dimethylacetamide (DMAC) and N-methylpynolidone (NMP); urea such as l,3-dimethyl-2-imidazolidinone (DMI); sulfoxides such as dimethylsulfoxide (DMSO); and others.
  • a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloro
  • the reaction temperatare can be optionally set depending on the compounds to be reacted.
  • the reaction temperature is usually, but not limited to, about 20°C to 50 °C.
  • the reaction may be conducted for, usually, 30 minutes to 24 hours and preferably 1 to 10 hours.
  • the reaction can be advantageously carried out in the presence of a base including, for instance, organic amines such as pyridine, triethylamine and NN-diisopropylethylamine, dimethylaniline, diethylaniline, 4-dimethylaminopyridine, and others.
  • a base including, for instance, organic amines such as pyridine, triethylamine and NN-diisopropylethylamine, dimethylaniline, diethylaniline, 4-dimethylaminopyridine, and others.
  • the compound of the formula (VH) (wherein Q_, Q 2 and Q 3 are the same as defined above and P . represents alkyl such as methyl or ethyl) can be prepared by the reduction of the compound of the formula (VI) (wherein Pi, Qi, Q 2 and Q 3 are the same as defined above and P represents amino or nitro).
  • the reduction can be carrid out by using the agent including, for instance, metal such as lithium, sodium, and the like.
  • the reaction can be carried out in a solvent including, for instance, liquid ammonia; alkylamine such as methylamine, ethylamine, and ethylenediamine (EDA); and alcohols such as methanol, ethanol, isopropanol, tert-butanol and others.
  • a solvent including, for instance, liquid ammonia; alkylamine such as methylamine, ethylamine, and ethylenediamine (EDA); and alcohols such as methanol, ethanol, isopropanol, tert-butanol and others.
  • a solvent including, for instance, liquid ammonia; alkylamine such as methylamine, ethylamine, and ethylenediamine (EDA); and alcohols such as methanol, ethanol, isopropanol, tert-butanol and others.
  • two or more of the solvents selected from the listed above can be mixed and used.
  • Solvent including, for instance, ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane can be used as a co-solvent.
  • ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane
  • THF tetrahydrofuran
  • 1,2-dimethoxyethane 1,2-dimethoxyethane
  • the reaction temperature can be optionally set depending on the compounds to be reacted.
  • the reaction temperature is usually, but not limited to, about -78°C to 50 °C.
  • the reaction may be conducted for, usually, 30 minutes to 24 hours and preferably 1 to 10 hours.
  • the compound of the formula (VIII) (wherein Qi, Q 2 and Q 3 are the same as defined above) can be prepared by the reaction of the compound of the formula (VH) (wherein Pi, Qi, Q 2 and Q 3 are the same as defined above are the same as defined above) with an acid such as hydrochloric acid.
  • the reaction may be canied out in a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane; ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; alcohols such as methanol, ethanol; water and others.
  • halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane
  • ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane
  • alcohols such as methanol, ethanol; water and others.
  • two or more of the solvents selected from the listed above can be mixed and used.
  • the reaction temperature can be optionally set depending on the compounds to be reacted.
  • the reaction temperature is usually, but not limited to, about 20°C to 100°C.
  • the reaction may be conducted for, usually, 30 minutes to 24 hours and preferably 1 to 10 hours.
  • the compound of the formula (II) (wherein Qi, Q 2 and Q 3 are the same as defined above) can be prepared by reacting the compound of the formula (VDT) (wherein Q 1 ⁇ Q 2 and Q 3 are the same as defined above) with a reducing agent such as sodium borohydride or lithium aluminum hydride.
  • a reducing agent such as sodium borohydride or lithium aluminum hydride.
  • the reaction may be carried out in a solvent including, for instance, ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; alcohols such as methanol, ethanol, isopropanol, and others.
  • ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane
  • alcohols such as methanol, ethanol, isoprop
  • the reaction temperature can be optionally set depending on the compounds to be reacted.
  • the reaction temperatare is usually, but not limited to, about 20°C to 50°C.
  • the reaction may be conducted for, usually, 30 minutes to 24 hours and preferably 1 to 10 hours.
  • the compound (VI) is commercially available or can be prepared by the use of known techniques.
  • the compound of the formula (VDT) can also be prepared by the following procedures.
  • the compound of the formula (X) (wherein Q Q 2 and Q 3 are the same as defined above) can be prepared by the nitration of the compound of the formula (IX) (wherein Qi, Q 2 and Q 3 are the same as defined above.) using the agent including, for instance, nitroric acid, potassium nitrate, a combination agent of dinifrogen pentoxide and sulphur dioxide, a combination agent of dinifrogen pentoxide, nitromethane and sodium bisulfonate, a combination agent of dimethylsulfoxide, acetic anhydride.
  • the agent including, for instance, nitroric acid, potassium nitrate, a combination agent of dinifrogen pentoxide and sulphur dioxide, a combination agent of dinifrogen pentoxide, nitromethane and sodium bisulfonate, a combination agent of dimethylsulfoxide, acetic anhydride.
  • the reaction can be carried out without solvent or in a solvent including, for instance, acid such as acetic acid, sulfonic acid, trifluoroacetic acid.
  • acid such as acetic acid, sulfonic acid, trifluoroacetic acid.
  • two or more of the solvents selected from the listed above can be mixed and used.
  • the reaction temperature can be optionally set depending on the compounds to be reacted.
  • the reaction temperature is usually, but not limited to, about -15°C to 100°C.
  • the reaction may be conducted for, usually, 30 minutes to 24 hours and preferably 1 to 10 hours.
  • the compound of the formula (XI) (wherein Q' ⁇ , Q' 2 and Q' 3 independently represent N, N ' -O " or CH, with the proviso that at least one of Q Q 2 and Q 3 is N-O " ) can be prepared by the oxydation of the compound of the formula (DC) (wherein Q_, Q 2 and Q 3 are the same as defined above) using an agent including, for instance, hydrogen peroxide, m-chloro- perbenzoic acid, dimethyldioxirane and the like.
  • the reaction can be carried out in a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane; acid such as acetic acid, and water.
  • a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane; acid such as acetic acid, and water.
  • halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane
  • acid such as acetic acid
  • the reaction temperature can be optionally set depending on the compounds to be reacted.
  • the reaction temperature is usually, but not limited to, about -15°C to 100°C.
  • the reaction may be conducted for, usually, 30 minutes to 24 hours and preferably 1 to 10 hours.
  • the compound of the formula (XII) (wherein Q' ⁇ , Q' 2 and Q' 3 are the same as defined above) can be prepared by the nitration of the compound of the formula (XT) (wherein Q'j, Q' 2 and Q' 3 are the same as defined above) in a similar manner as described for the preparation of the compound of the formula (X).
  • the compound of the formula (X) (wherein Qi, Q 2 and Q 3 are the same as defined above) can be prepared by the reduction of the compound of the formula (XH) (wherein Q'j, Q' 2 and Q' 3 are the same as defined above) using the agent including, for instance, triphenyl phosphine, xriethyl phosphite, trimethyl phosphite, methanesulfonyl chloride, a combination agent of lithium chloride and sodium borohydride, and the like.
  • the agent including, for instance, triphenyl phosphine, xriethyl phosphite, trimethyl phosphite, methanesulfonyl chloride, a combination agent of lithium chloride and sodium borohydride, and the like.
  • the reaction can be carried out in a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane; ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; aromatic hydrocarbons such as benzene, toluene and xylene, and the like.
  • halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane
  • ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane
  • aromatic hydrocarbons such as benzene, toluene and xylene, and the like.
  • two or more of the solvents selected from the listed above can be mixed and used.
  • the reaction temperature can be
  • the compound of the formula (VIH) (wherein Qi, Q 2 and Q 3 are the same as defined above) can be prepared by reducing nitro group of the compound of the formula (X) (wherein Q Q 2 and Q 3 are the same as defined above.) using an agent including, for instance, metals such as zinc and iron in the presence of acid including, for instance, hydrochloric acid and acetic acid and stannous chloride, or by hydrogenation using a catalyst including, for instance, palladium on carbon and platinum on carbon.
  • an agent including, for instance, metals such as zinc and iron in the presence of acid including, for instance, hydrochloric acid and acetic acid and stannous chloride, or by hydrogenation using a catalyst including, for instance, palladium on carbon and platinum on carbon.
  • the reaction can be carried out in a solvent including, for instance, ethers such as diethyl ether, isopropyl ether, dioxane, tetrahydrofuran (THF) and 1,2-dimethoxyethane, aromatic hydrocarbons such as benzene, toluene and xylene, alcohols such as methanol, ethanol, 1-propanol, isopropanol and tert-butanol, water and others.
  • ethers such as diethyl ether, isopropyl ether, dioxane, tetrahydrofuran (THF) and 1,2-dimethoxyethane
  • aromatic hydrocarbons such as benzene, toluene and xylene
  • alcohols such as methanol, ethanol, 1-propanol, isopropanol and tert-butanol, water and others.
  • the reaction temperature can be optionally set depending on the compounds to be reacted.
  • the reaction temperatare is usually, but not limited to, about 20°C to 120°C.
  • the reaction may be conducted for, usually, 30 minutes to 24 hours and preferably 1 to 10 hours.
  • the compound of the formula (VHT) (wherein Qi, Q 2 and Q 3 are the same as defined above) can be prepared by reduction of the compound of the formula (XIT) (wherein Q'_, Q' 2 and Q' 3 are the same as defined above) as shown in the Step ii-3.
  • the reduction can be carried out using an agent including, for instance, metals such as titanium and iron, and sodium hypophosphite together with a catalyst including, for instance, palladium on carbon and platinum on carbon.
  • an agent including, for instance, metals such as titanium and iron, and sodium hypophosphite together with a catalyst including, for instance, palladium on carbon and platinum on carbon.
  • the reaction can be canied out in a solvent including, for instance, ethers such as diethyl ether, isopropyl ether, dioxane, tetrahydrofuran (THF) and 1,2-dimethoxyethane, aromatic hydrocarbons such as benzene, toluene and xylene, alcohols such as methanol, ethanol, 1-propanol, isopropanol and tert-butanol, acid such as acetic acid, water and others.
  • ethers such as diethyl ether, isopropyl ether, dioxane, tetrahydrofuran (THF) and 1,2-dimethoxyethane
  • aromatic hydrocarbons such as benzene, toluene and xylene
  • alcohols such as methanol, ethanol, 1-propanol, isopropanol and tert-butanol
  • acid such as acetic acid, water and others.
  • the reaction temperature can be optionally set depending on the compounds to be reacted.
  • the reaction temperature is usually, but not limited to, about 20°C to 120°C.
  • the reaction may be conducted for, usually, 30 minutes to 24 hours and preferably 1 to 10 hours.
  • the compound (IX) is commercially available or can be prepared by the use of known techniques.
  • the compound of the formula (VIH) (wherein Qi, Q 2 and Q 3 are the same as defined above) can be prepared by the reaction of the compound of the formula (XHI) (wherein Q Q 2 and Q 3 are the same as defined above.) using the agent including, for instance, p- toluenesulfonyl isocyanate.
  • the reaction can be carried in a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane; ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; aromatic hydrocarbons such as benzene, toluene and xylene and others.
  • halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane
  • ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane
  • aromatic hydrocarbons such as benzene, toluene and xylene and others.
  • two or more of the solvents selected from the listed above can be mixed and used.
  • the reaction temperature can be optionally set depending on the compounds to be reacted.
  • the reaction temperatare is usually, but not limited to, about 20 °C to 100 °C.
  • the reaction may be conducted for, usually, 30 minutes to 24 hours and preferably 1 to 10 hours.
  • the compound of the formula (XIV) (wherein Q_, Q 2 and Q 3 are the same as defined above and L 2 represents a leaving group including halogen atom such as chlorine, bromine, or iodine atom; and alkylsulfonyloxy such as frifluoromethylsulfonyloxy) can be prepared by the reaction of the compound of the formula (XHI) (wherein Q 1 ⁇ Q 2 and Q 3 are the same as defined above.) using the agent including, for instance, halogenating reagent such as POCl 3 , POBr 3 , PC1 5 and the like; or sulfonyl chloride such as xrifluoromethylsulfonyl chloride.
  • halogenating reagent such as POCl 3 , POBr 3 , PC1 5 and the like
  • sulfonyl chloride such as xrifluoromethylsulfonyl chloride.
  • the reaction may be carried out in a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and l,2-dichloroethane; such as ethers such as dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; aromatic hydrocarbons such as benzene, toluene, and xylene, and others.
  • halogenated hydrocarbons such as dichloromethane, chloroform and l,2-dichloroethane
  • ethers such as dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane
  • aromatic hydrocarbons such as benzene, toluene, and xylene, and others.
  • two or more of the solvents selected from the listed above can be mixed and used.
  • the reaction can be advantageously conducted in the presence of a base, including, for instance, such as pyridine, triethylamine and NN-diisopropylethylamine, dimethylaniline, diethylaniline, and others.
  • a base including, for instance, such as pyridine, triethylamine and NN-diisopropylethylamine, dimethylaniline, diethylaniline, and others.
  • the reaction temperatare is usually, but not limited to, about 40°C to 200°C and preferably about 20°C to 180°C.
  • the reaction may be conducted for, usually, 30 minutes to 24 hours and preferably 2 hours to 10 hours.
  • the compound of the formula (XIV) (wherein L 2 , Q_, Q 2 and Q 3 are the same as defined above) can be prepared by the reaction of the compound of the formula (Xlfl) (wherein Qi, Q 2 and Q 3 are the same as defined above) using the agent including, for instance, ammonia.
  • the reaction can be advantageously conducted in the presence of a catalyst including, for instance, copper(T) oxide, copper(D) sulfate and the like.
  • a catalyst including, for instance, copper(T) oxide, copper(D) sulfate and the like.
  • the reaction may be canied out in a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane; such as ethers such as dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; aromatic hydrocarbons such as benzene, toluene, and xylene, and others.
  • halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane
  • ethers such as dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane
  • aromatic hydrocarbons such as benzene, toluene, and xylene, and others.
  • two or more of the solvents selected from the listed above can be mixed and used.
  • the reaction temperature is usually, but not limited to, about 40°C to 200°C and preferably about 20°C to 180°C.
  • the reaction may be conducted for, usually, 30 minutes to 24 hours and preferably 2 hours to 12 hours.
  • the compound of the formula (VIH) can also be prepared by the following procedures.
  • the compound of the formula (XVI) (wherein Qi, Q 2 and Q 3 are the same as defined above; and P 3 represents aralkyl such as benzyl, 4-methoxybenzyl, 3,4-dimethoxybenzyl) can be prepared by the reaction of the compound of the formula (XIV) (wherein L 2 , Qi, Q 2 and Q 3 are the same as defined above) with the compound of the formula (XV) (wherein P 3 is the same as defined above).
  • the reaction can be carried out in the presence of a palladium catalyst such as tetrakis- (triphenylphosphine)palladium or a combination of a phosphine ligand and a palladium catalyst such as tri-o-tolylphosphine and palladium (H) acetate.
  • a palladium catalyst such as tetrakis- (triphenylphosphine)palladium or a combination of a phosphine ligand and a palladium catalyst such as tri-o-tolylphosphine and palladium (H) acetate.
  • the reaction can be advantageously carried out in the presence of a base including, for instance, cesium carbonate, sodium carbonate, potassium carbonate, barium hydroxide sodium methoxide, sodium ethoxide, potassium tert-butoxide and the like.
  • This reaction can be carried out in a solvent including, for instance, alcohol such as methanol, ethanol, 1-propanol, isopropanol and tert-butanol; ethers, such as dioxane, isopropyl ether, diethyl ether, 1,2-dimethoxyethane and tetrahydrofuran (THF); aromatic hydrocarbons such as benzene, toluene and xylene; nitriles such as acetonitrile; amides such as dimethylformamide (DMF) N, ⁇ - dimethylacetamide and ⁇ -methylpynolidone; sulfoxides such as dimethylsulfoxide (DMSO); water and others.
  • a solvent including, for instance, alcohol such as methanol, ethanol, 1-propanol, isopropanol and tert-butanol; ethers, such as dioxane, isopropyl ether, diethyl
  • the reaction temperature can be optionally set depending on the compounds to be reacted.
  • the reaction temperature is usually, but not limited to, about 10°C to 200°C and preferably about 50°C to 150°C.
  • the reaction may be conducted for, usually, 30 minutes to 24 hours and preferably 1 to 12 hours.
  • the compound of the formula (VIH) (wherein Qi, Q 2 and Q 3 are the same as defined above) can be prepared by the removal of P 3 of the compound of the formula (XVI) (wherein P 3 , Qi, Q 2 and Q 3 are the same as defined above).
  • the removal of P 3 can be done by hydrogenation using a catalyst including, for instance, palladium on carbon and palladium hydroxide. Also, the removal can be done by using a reagent including, for instance, trifluoroacetic acid, eerie ammonium nitrate (CAN) or 2,3-dichloro-5,6-dicyano-l,4- benzoquinone (DDQ), when P 3 is 4-methoxybenzyl or 3,4-dimethoxybenzyl.
  • a catalyst including, for instance, palladium on carbon and palladium hydroxide.
  • a reagent including, for instance, trifluoroacetic acid, eerie ammonium nitrate (CAN) or 2,3-dichloro-5,6-dicyano-l,4- benzoquinone (DDQ), when P 3 is 4-methoxybenzyl or 3,4-dimethoxybenzyl.
  • This reaction can be carried out in a solvent including, for instance, alcohol such as methanol, ethanol, 1-propanol, isopropanol and tert-butanol; ethers, such as dioxane, isopropyl ether, diethyl ether, 1,2-dimethoxyethane and tetrahydrofuran (THF); aromatic hydrocarbons such as benzene, toluene and xylene; ester such as ethyl acetate; water and others.
  • a solvent including, for instance, alcohol such as methanol, ethanol, 1-propanol, isopropanol and tert-butanol; ethers, such as dioxane, isopropyl ether, diethyl ether, 1,2-dimethoxyethane and tetrahydrofuran (THF); aromatic hydrocarbons such as benzene, toluene and xylene; ester such as e
  • the compound of the formula (I) (wherein m, p, Qi, Q 2 , Q 3 , R and X are the same as defined above) can alternatively be prepared by the following procedures in three steps;
  • the compound of the formula (XVH) (wherein m, p, Pi, Q l5 Q 2 , Q 3 , R and X are the same as defined above) can be prepared in a similar manner as described in Method [A], [B], [C], [D] or [E] for the preparation of the compound of the formula (I) by using a compound of the formula (VH) (wherein Pi, Q Q 2 and Q 3 are the same as defined above) instead of the compound of the formula (H).
  • the compound of the formula (XVHI) (m, p, Qi, Q 2 , Q 3 , R and X are the same as defined above) can be prepared by reacting the compound of the formula (XVH) (m, p, P b Qi, Q 2 , Q 3 , R and X are the same as defined above) with an acid such as hydrochloric acid.
  • the reaction may be canied out in a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane; ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; alcohols such as methanol, ethanol; water and others.
  • halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane
  • ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane
  • alcohols such as methanol, ethanol; water and others.
  • two or more of the solvents selected from the listed above can be mixed and used.
  • the reaction temperatare can be optionally set depending on the compounds to be reacted.
  • the reaction temperatare is usually, but not limited to, about 20°C to 100°C.
  • the reaction may be conducted for, usually, 30 minutes to 24 hours and preferably 1 to 10 hours.
  • the compound of the formula (I) (wherein m, p, Q_, Q 2 , Q 3 , R and X are the same as defined above) can be prepared by reacting the compound of the formula (XVIH) (wherein m, p, Qi. Q 2 . Q 3. and X are the same as defined above) with reducing agent such as sodium borohydride or lithium aluminum hydride.
  • the reaction may be carried out in a solvent including, for instance, ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; alcohols such as methanol, ethanol, isopropanol, and others.
  • ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane
  • alcohols such as methanol, ethanol, isopropanol, and others.
  • two or more of the solvents selected from the listed above can be mixed and used.
  • the reaction temperatare can be optionally set depending on the compounds to be reacted.
  • the reaction temperature is usually, but not limited to, about 20°C to 50°C.
  • the reaction may be conducted for, usually, 30 minutes to 24 hours and preferably 1 to 10 hours.
  • the compound of the formula (XVHI) (m, p, Qi, Q 2 , Q 3 , R and X are the same as defined above) can alternatively be prepared in a similar manner as described in Method [A], [B], [C], [D] or [E] for the preparation of the compound of the formula (I) by using a compound of the formula (VHT) (wherein Qi, Q 2 and Q 3 are the same as defined above) instead of the compound of the formula (H).
  • the compound of the formula (I-a) (wherein m, p, Qi, Q 2 , Q 3 and R are the same as defined above and X' is -O-, or N(R J )-) can be prepared by the following procedures.
  • the compound of the formula (XXI) (wherein m, Qi, Q 2 and Q 3 are the same as defined above and L 3 represents leaving group including, for instance, halogen atom such as chlorine, bromine, or iodine atom) can be prepared in a similar manner as described in Method [A], [B], [C], [D] or [E] for the preparation of the compound of the formula (I) by using a compound of the formula (XIX) (wherein m and L 3 are the same as defined above) instead of the compound of the formula (IV), or using a compound of the formula (XX) (wherein m and L 3 are the same as defined above) instead of the compound of the formula (V).
  • halogen atom such as chlorine, bromine, or iodine atom
  • the compound of the formula (I-a) (wherein m, p, Qi, Q 2 , Q 3 , R and X' are the same as defined above) can be prepared by reacting the compound of the formula (XXI) (wherein m, L 3 , Qi, Q 2 and Q 3 are the same as defined above) and the compound of the formula (XXII) (wherein p, R and X' are the same as defined above).
  • the reaction may be carried out in a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane; ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; aromatic hydrocarbons such as benzene, toluene and xylene; nitriles such as acetonitrile; amides such as N,N- dimethylformamide (DMF), NN-dimethylacetamide (DMAC) and N-methylpynolidone (NMP); ureas such as l,3-dimethyl-2-imidazolidinone (DMI); sulfoxides such as dimethylsulfoxide (DMSO); and others.
  • a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloro
  • the reaction temperature can be optionally set depending on the compounds to be reacted.
  • the reaction temperatare is usually, but not limited to, about 0°C to 50°C.
  • the reaction may be conducted for, usually, 30 minutes to 24 hours and preferably 1 to 10 hours.
  • the reaction can be advantageously carried out in the presence of a base including, for instance, organic amines such as pyridine, triethylamine and NN-diisopropylethylamine, dimethylaniline, diethylaniline, 4-dimethylaminopyridine, and others.
  • a base including, for instance, organic amines such as pyridine, triethylamine and NN-diisopropylethylamine, dimethylaniline, diethylaniline, 4-dimethylaminopyridine, and others.
  • the stereoisomeric form of the compound (I), R form (I-a) (wherein m, p, Qi, Q 2 , Q 3 , R and X are the same as defined above) can be prepared in a similar manner as described in Method [A], [B], [C], [D], or [E] for the preparation of the compound of the formula (I) by using a compound of the formula (H-a) (wherein Q l9 Q 2 and Q 3 are the same as defined above) instead of the compound of the formula (H).
  • the stereoisomeric form of the compound (I), S form (I-a') (wherein m, p, Qi, Q 2 , Q 3 , R and X are the same as defined above) can be prepared in the similar manner as described in Method [A], [B], [C], [D], or [E] for the preparation of the compound of the formula (I) by using a compound of the formula (H-a') (wherein Qi, Q 2 and Q 3 are the same as defined above) instead of the compound of the formula (IT).
  • the compound (H-a) or (H-a') can be prepared by the use of known techniques.
  • Typical salts of the compound shown by the formula (A) include salts prepared by reaction of the compounds of the present invention with a mineral or organic acid, or an organic or inorganic base. Such salts are known as acid addition and base addition salts, respectively.
  • Acids to form acid addition salts include inorganic acids such as, without limitation, sulfuric acid, phosphoric acid, hydrochloric acid, hydrobromic acid, hydriodic acid and the like, and organic acids, such as, without limitation, p-toluenesulfonic acid, methanesulfonic acid, oxalic acid, p- bromophenylsulfonic acid, carbonic acid, succinic acid, citric acid, benzoic acid, acetic acid, and the like.
  • inorganic acids such as, without limitation, sulfuric acid, phosphoric acid, hydrochloric acid, hydrobromic acid, hydriodic acid and the like
  • organic acids such as, without limitation, p-toluenesulfonic acid, methanesulfonic acid, oxalic acid, p- bromophenylsulfonic acid, carbonic acid, succinic acid, citric acid, benzoic acid, acetic acid, and the like.
  • Base addition salts include those derived from inorganic bases, such as, without limitation, ammonium hydroxide, alkaline metal hydroxide, alkaline earth metal hydroxides, carbonates, bicarbonates, and the like, and organic bases, such as, without limitation, ethanolamine, triethylamine, tris(hydroxymethyl)aminomethane, and the like.
  • inorganic bases include sodium hydroxide, potassium hydroxide, potassium carbonate, sodium carbonate, sodium bicarbonate, potassium bicarbonate, calcium hydroxide, calcium carbonate, and the like.
  • the compound of the present invention or a salt thereof, depending on its substituents, may be modified to form lower alkylesters or known other esters; and/or hydrates or other solvates. Those esters, hydrates, and solvates are included in the scope of the present invention.
  • the compound of the present invention may be administered in oral forms, such as, without limitation normal and enteric coated tablets, capsules, pills, powders, granules, elixirs, tinctures, solution, suspensions, syrups, solid and liquid aerosols and emulsions. They may also be administered in parenteral forms, such as, without limitation, intravenous, intraperitoneal, subcutaneous, intramuscular, and the like forms, well-known to those of ordinary skill in the pharmaceutical arts.
  • the compounds of the present invention can be administered in infranasal form via topical use of suitable infranasal vehicles, or via transdermal routes, using transdermal delivery systems well-known to those of ordinary skilled in the art.
  • the dosage regimen with the use of the compounds of the present invention is selected by one of ordinary skill in the arts, in view of a variety of factors, including, without limitation, age, weight, sex, and medical condition of the recipient, the severity of the condition to be treated, the route of administration, the level of metabolic and excretory function of the recipient, the dosage form employed, the particular compound and salt thereof employed.
  • the compounds of the present invention are preferably formulated prior to administration together with one or more pharmaceutically-acceptable excipients.
  • Excipients are inert substances such as, without limitation carriers, diluents, flavoring agents, sweeteners, lubricants, solubilizers, suspending agents, binders, tablet disintegrating agents and encapsulating material.
  • compositions of the present invention are pharmaceutical formulation comprising a compound of the invention and one or more pharmaceutically-acceptable excipients that are compatible with the other ingredients of the formulation and not deleterious to the recipient thereof.
  • Pharmaceutical formulations of the invention are prepared by combining a therapeutically effective amount of the compounds of the invention together with one or more pharmaceutically- acceptable excipients therefore.
  • the active ingredient may be mixed with a diluent, or enclosed within a carrier, which may be in the form of a capsule, sachet, paper, or other container.
  • the carrier may serve as a diluent, which may be solid, semi-solid, or liquid material which acts as a vehicle, or can be in the form of tablets, pills powders, lozenges, elixirs, suspensions, emulsions, solutions, syrups, aerosols, ointments, containing, for example, up to 10% by weight of the active compound, soft and hard gelatin capsules, suppositories, sterile injectable solutions and sterile packaged powders.
  • a diluent which may be solid, semi-solid, or liquid material which acts as a vehicle, or can be in the form of tablets, pills powders, lozenges, elixirs, suspensions, emulsions, solutions, syrups, aerosols, ointments, containing, for example, up to 10% by weight of the active compound, soft and hard gelatin capsules, suppositories, sterile injectable solutions and sterile packaged powders.
  • the active ingredient may be combined with an oral, and non-toxic, pharmaceutically-acceptable carrier, such as, without limitation, lactose, starch, sucrose, glucose, sodium carbonate, mannitol, sorbitol, calcium carbonate, calcium phosphate, calcium sulfate, methyl cellulose, and the like; together with, optionally, disintegrating agents, such as, without limitation, maize, starch, methyl cellulose, agar bentonite, xanthan gum, alginic acid, and the like; and optionally, binding agents, for example, without limitation, gelatin, natural sugars, beta- lactose, com sweeteners, natural and synthetic gums, acacia, tragacanth, sodium alginate, carboxymethylcellulose, polyethylene glycol, waxes, and the like; and, optionally, lubricating agents, for example, without limitation, magnesium stearate, sodium stearate, stearic acid, sodium oleate, sodium benzoate
  • the carrier may be a finely divided solid which is in admixture with the finely divided active ingredient.
  • the active ingredient may be mixed with a carrier having binding properties in suitable proportions and compacted in the shape and size desired to produce tablets.
  • the powders and tablets preferably contain from about 1 to about 99 weight percent of the active ingredient which is the novel composition of the present invention.
  • Suitable solid carriers are magnesium carboxymethyl cellulose, low melting waxes, and cocoa butter.
  • Sterile liquid formulations include suspensions, emulsions, syrups and elixirs.
  • the active ingredient can be dissolved or suspended in a pharmaceutically acceptable earners, such as sterile water, sterile organic solvent, or a mixture of both sterile water and sterile organic solvent.
  • the active ingredient can also be dissolved in a suitable organic solvent, for example, aqueous propylene glycol.
  • a suitable organic solvent for example, aqueous propylene glycol.
  • Other compositions can be made by dispersing the finely divided active ingredient in aqueous starch or sodium carboxymethyl cellulose solution or in a suitable oil.
  • the formulation may be in unit dosage form, which is a physically discrete unit containing a unit dose, suitable for administration in human or other mammals.
  • a unit dosage form can be a capsule or tablets, or a number of capsules or tablets.
  • a "unit dose" is a predetermined quantity of the active compound of the present invention, calculated to produce the desired therapeutic effect, in association with one or more excipients.
  • the quantity of active ingredient in a unit dose may be varied or adjusted from about 0.1 to about 1000 milligrams or more according to the particular treatment involved.
  • Typical oral dosages of the present invention when used for the indicated effects, will range from about O.Olmg /kg/day to about 100 mg/kg/day, preferably from 0.1 mg/kg/day to 30 mg/kg/day, and most preferably from about 0.5 mg/kg/day to about 10 mg/kg/day.
  • parenteral administration it has generally proven advantageous to administer quantities of about 0.001 to lOOmg /kg/day, preferably from 0.01 mg/kg/day to 1 mg/kg/day.
  • the compounds of the present invention may be administered in a single daily dose, or the total daily dose may be administered in divided doses, two, three, or more times per day. Where delivery is via transdermal forms, of course, administration is continuous.
  • Instrument MS Micromass ZQ
  • instrument HPLC Waters Alliance 2795
  • eluent A water + 500 ⁇ l 50% aqueous formic acid / 1
  • eluent B acetonifrile + 500 ⁇ l 50% aqueous formic acid / 1
  • oven temp. 35°C
  • flow rate 0.0 min 1.0 m_/min- 3.0 min 3.0 ml/min- 4.0 min 3.0 ml/min
  • UV-detection 210 nm.
  • Instrument MS Micromass ZQ; instrument HPLC: HP 1100 Series; UV DAD; column: Grom-Sil 120 ODS-4 HE 50 mm x 2 mm, 3.0 ⁇ m; eluent A: water + 500 ⁇ l 50% aqueous formic acid / 1, eluent B: acetonifrile + 500 ⁇ l 50% aqueous formic acid / 1; gradient: 0.0 min 0%>B - 2.9 min 70%B -> 3.1 min 90%B -» 4.5 min 90%B; oven temp.: 50 °C; flow rate: 0.8 ml min; UV- detection: 210 nm.
  • Preparative HPLC purifications are performed on a GROM-SIL 120 ODS-4 HE 10 ⁇ m, 250 mm x 30 mm column with acetonitrile/water gradients.
  • H NMR spectra were recorded using either Bruker DRX-300 (300 MHz for H) spectrometer or Bracker 500 UlfraShieledTM (500 MHz for IH). Chemical shifts are reported in parts per million (ppm) with tetramethylsilane (TMS) as an internal standard at zero ppm. Coupling constant (J) are given in hertz and the abbreviations s, d, t, q, m, and br refer to singlet, doblet, triplet, quartet, multiplex, and broad, respectively.
  • TLC was performed on a precoated silica gel plate (Merck silica gel 60 F-254).
  • Silica gel WAKO- gel C-200 (75-150 ⁇ m) was used for all column chromatography separations. All chemicals were reagent grade and were purchased from Sigma-Aldrich, Wako pure chemical industries, Ltd., Great Britain, Tokyo kasei kogyo Co., Ltd., Nacalai tesque, Inc., Watanabe Chemical Ind. Ltd., Maybridge pic, Lancaster Synthesis Ltd., Merck KgaA, Germany, or Kanto Chemical Co., Ltd.
  • Human vanilloid receptor (hVRl) cDNA was cloned from libraries of axotomized dorsal root ganglia (WO 00/29577). The cloned hVRl cDNA was constructed with pcDNA3 vector and transfected into a CH01uc9aeq cell line. The cell line contains aequorin and CRE-luciferase reporter genes as read-out signals.
  • the fransfectants were cloned by limiting dilution in selection medium (DMEM/F12 medium (Gibco BRL) supplemented with 10% FCS, 1.4 mM Sodium pyruvate, 20 mM HEPES, 0.15% Sodium bicarbonate, 100 U/ml penicillin, 100 ⁇ g/ml streptomycin, 2 mM glutamine, non-essential amino acids and 2 mg/ml G418). Ca 2+ influx was examined in the capsaicin-stimulated clones. A high responder clone was selected and used for further experiments in the project.
  • the human VRl-CH01uc9aeq cells were maintained in the selection medium and passaged every 3-4 days at l-2.5xl0 5 cells/flask (75 mm 2 ).
  • Human VRl-CH01uc9aeq cells were suspended in a culture medium which is the same as the selection medium except for G418 and seeded at a density of 1,000 cells per well into 384-well plates (black walled clear-base / ⁇ alge ⁇ unc International). Following the culture for 48 hrs the medium was changed to 2 ⁇ M Fluo-3 AM (Molecular Probes) and 0.02% Puronic F-127 in assay buffer (Hank's balanced salt solution (HBSS), 17 mM HEPES ( ⁇ H7.4), 1 mM Probenecid, 0.1% BSA) and the cells were incubated for 60 min at 25°C. After washing twice with assay buffer the cells were incubated with a test compound or vehicle for 20 min at 25°C.
  • assay buffer Hort's balanced salt solution (HBSS), 17 mM HEPES ( ⁇ H7.4), 1 mM Probenecid, 0.1% BSA
  • DRG dorsal root ganglia
  • DRG was incubated with 0.1% trypsin (Gibco BRL) in PBS(-) (Gibco BRL) for 30 min at 37°C, then a half volume of fetal calf serum (FCS) was added and the cells were spun down.
  • FCS fetal calf serum
  • the DRG neuron cells were resuspended in Ham F12/5% FCS/5% horse serum (Gibco BRL) and dispersed by repeated pipetting and passing through 70 ⁇ m mesh (Falcon). The culture plate was incubated for 3 hours at 37°C to remove contaminating Schwann cells.
  • ⁇ on-adherent cells were recovered and further cultured in laminin-coated 384 well plates ( ⁇ unc) at lxlO 4 cells/50 ⁇ l well for 2 days in the presence of 50 ng/ml recombinant rat NGF (Sigma) and 50 ⁇ M 5-fluorodeoxyuridine (Sigma).
  • Human P2X1 -transfected CH01uc9aeq cell line was established and maintained in Dulbecco's modified Eagle's medium (DMEM/F12) supplemented with 7.5% FCS, 20 mM HEPES-KOH (pH 7.4), 1.4 mM sodium pyruvate, 100 U/ml penicillin, 100 ⁇ g/ml streptomycin, 2 mM glutamine (Gibco BRL) and 0.5 Units/ml apyrase (grade I, Sigma).
  • the suspended cells were seeded in each well of 384-well optical bottom black plates (Nalge Nunc International) at 3 x 10 3 / 50 ⁇ l / well. The cells were cultured for following 48 hrs to adhere to the plates.
  • P2X1 receptor agonist-mediated increases in cytosolic Ca 2+ levels were measured using a fluorescent Ca 2+ chelating dye, Fluo-3 AM (Molecular Probes).
  • the plate-attached cells were washed twice with washing buffer (HBSS, 17 mM HEPES-KOH (pH 7.4), 0.1% BSA and 0.5 units/ml apyrase), and incubated in 40 ⁇ l of loading buffer (1 ⁇ M Fluo-3 AM, 1 mM probenecid, 1 ⁇ M cyclosporin A, 0.01% pluronic (Molecular Probes)in washing buffer) for 1 hour in a dark place.
  • Rats were anesthetized by intraperitoneal adminisfration of urethane (Sigma) at 1.2 g/kg.
  • the abdomen was opened through a midline incision, and a polyethylene catheter (BECTON DICKINSON, PE50) was implanted into the bladder through the dome.
  • a polyethylene catheter (Hibiki, size 5) filled with 2 IU / ml of heparin ( ⁇ ovo Heparin, Aventis Pharma) in saline (Otsuka) was inserted into a common iliac artery.
  • the bladder catheter was connected via T-tube to a pressure transducer (Viggo- Spectramed Pte Ltd, DT-XXAD) and a microinjection pump (TERUMO). Saline was infused at room temperature into the bladder at a rate of 2.4 ml/hr. fritravesical pressure was recorded continuously on a chart pen recorder (Yokogawa). At least three reproducible mictarition cycles, conesponding to a 20-minute period, were recorded before a test compound administration and used as baseline values.
  • the saline infusion was stopped before administrating compounds.
  • a testing compound dissolved in the mixture of ethanol, Tween 80 (IC ⁇ Biomedicals Inc.) and saline (1 : 1 : 8, v/v/v) was administered mtraarterially at 10 mg/kg. 2min after the administration of the compound 10 ⁇ g of capsaicin ( ⁇ acalai Tesque) dissolved in ethanol was administered mtraarterially.
  • Cyclo- phosphamide (CYP) dissolved in saline was administered infraperitoneally at 150 mg/kg 48 hours before experiment.
  • Rats were anesthetized by intraperitoneal administration of urethane (Sigma) at 1.25 g/kg. The abdomen was opened through a midline incision, and a polyethylene catheter (BECTON DICKINSON PE50) was implanted into the bladder through the dome. In parallel, the inguinal region was incised, and a polyethylene catheter (BECTON DICKINSON, PE50) filled with saline (Otsuka) was inserted into a femoral vein. After the bladder was emptied, the rats were left for 1 hour for recovery from the operation.
  • urethane Sigma
  • the bladder catheter was connected via T-tabe to a pressure transducer (Viggo- Spectramed Pte Ltd, DT-XXAD) and a microinjection pump (TERUMO). Saline was infused at room temperature into the bladder at a rate of 3.6 ml/hr for 20 min. Intravesical pressure was recorded continuously on a chart pen recorder (Yokogawa). At least three reproducible micturition cycles, conesponding to a 20-minute period, were recorded before a test compound administration.
  • a testing compound dissolved in the mixture of ethanol, Tween 80 (ICN Biomedicals Inc.) and saline (1 : 1 : 8, v/v/v) was administered intravenously at 0.05 mg/kg, 0.5 mg/kg or 5 mg/kg.
  • saline Nacalai Tesque was infused at room temperatare into the bladder at a rate of 3.6 ml/hr.
  • the cystometry parameters were analyzed as described previously [ Lecci A et al: Eur. J. Pharmacol. 259: 129-135, 1994].
  • the micturition frequency calculated from micturition interval and the bladder capacity calculated from a volume of infused saline until the first mictarition were analyzed from the cystometry data.
  • the testing compounds-mediated inhibition of the frequency and the testing compounds-mediated increase of bladder capacity were evaluated using unpaired Student's t-test. A probability levels less than 5% was accepted as significant difference. Data were analyzed as the mean ⁇ SEM from 4 - 7 rats.
  • Acute pain is measured on a hot plate mainly in rats.
  • Two variants of hot plate testing are used: In the classical variant animals are put on a hot surface (52 to 56 °C) and the latency time is measured until the animals show nociceptive behavior, such as stepping or foot licking.
  • the other variant is an increasing temperature hot plate where the experimental animals are put on a surface of neutral temperature. Subsequently this surface is slowly but constantly heated until the animals begin to lick a hind paw. The temperature which is reached when hind paw licking begins is a measure for pain threshold.
  • Compounds are tested against a vehicle treated control group. Substance application is performed at different time points via different application routes (i.v., i.p., p.o., i. , i.c.v., s.c, intradermal, transdermal) prior to pain testing.
  • application routes i.v., i.p., p.o., i. , i.c.v., s.c, intradermal, transdermal
  • Persistent pain is measured with the formalin or capsaicin test, mainly in rats.
  • a solution of 1 to 5% formalin or 10 to 100 ⁇ g capsaicin is injected into one hind paw of the experimental animal.
  • the animals show nociceptive reactions like flinching, licking and biting of the affected paw.
  • the number of nociceptive reactions within a time frame of up to 90 minutes is a measure for intensity of pain.
  • Compounds are tested against a vehicle treated control group. Substance application is performed at different time points via different application routes (i.v., i.p., p.o., i.t., i.c.v., s.c, intradermal, transdermal) prior to formalin or capsaicin administration.
  • application routes i.v., i.p., p.o., i.t., i.c.v., s.c, intradermal, transdermal
  • Neuropathic pain is induced by different variants of unilateral sciatic nerve injury mainly in rats.
  • the operation is performed under anesthesia.
  • the first variant of sciatic nerve injury is produced by placing loosely constrictive ligatures around the common sciatic nerve (Bennett and Xie, Pain 33 (1988): 87-107).
  • the second variant is the tight ligation of about the half of the diameter of the common sciatic nerve (Seltzer et al., Pain 43 (1990): 205-218).
  • a group of models is used in which tight ligations or fransections are made of either the L5 and L6 spinal nerves, or the L5 spinal nerve only (KIM SH; CHUNG JM, AN EXPERIMENTAL-MODEL FOR PERIPHERAL NEUROPATHY PRODUCED BY SEGMENTAL SPINAL NERVE LIGATION IN THE RA, PAIN 50 (3) (1992): 355-363).
  • the fourth variant involves an axotomy of two of the three terminal branches of the sciatic nerve (tibial and common peroneal nerves) leaving the remaining sural nerve intact whereas the last variant comprises the axotomy of only the tibial branch leaving the sural and common nerves uninjured. Confrol animals are treated with a sham operation.
  • the nerve injured animals develop a chronic mechanical allodynia, cold allodynia, as well as a thermal hyperalgesia.
  • Mechanical allodynia is measured by means of a pressure transducer (electronic von Frey Anesthesiometer, HTC fric.-Life Science Instruments, Woodland Hills, SA, USA; Electronic von Frey System, Somedic Sales AB, H ⁇ rby, Sweden).
  • Thermal hyperalgesia is measured by means of a radiant heat source (Plantar Test, Ugo Basile, Comerio, Italy), or by means of a cold plate of 5 to 10°C where the nocifensive reactions of the affected hind paw are counted as a measure of pain intensity.
  • a further test for cold induced pain is the counting of nocifensive reactions, or duration of nocifensive responses after plantar adminisfration of acetone to the affected hind limb.
  • Chronic pain in general is assessed by registering the circadanian rhytms in activity (Surjo and Amdt, Universitat zu K ⁇ ln, Cologne, Germany), and by scoring differences in gait (foot print patterns; FOOTPPTNTS program, Klapdor et al., 1997. A low cost method to analyse footprint patterns. J. Neurosci. Methods 75, 49-54).
  • Substance application is performed at different time points via different application routes (i.v., i.p., p.o., i.t., i.c.v., s.c, intradermal, transdermal) prior to pain testing.
  • application routes i.v., i.p., p.o., i.t., i.c.v., s.c, intradermal, transdermal
  • Inflammatory pain is induced mainly in rats by injection of 0.75 mg carrageenan or complete Freund's adjuvant into one hind paw.
  • the animals develop an edema with mechanical allodynia as well as thermal hyperalgesia.
  • Mechanical allodynia is measured by means of a pressure transducer (electronic von Frey Anesthesiometer, HTC Lie-Life Science Instruments, Woodland Hills, SA, USA).
  • Thermal hyperalgesia is measured by means of a radiant heat source (Plantar Test, Ugo Basile, Comerio, Italy, Paw thermal stimulator, G. Ozaki, University of California, USA).
  • Plant Test Ugo Basile, Comerio, Italy
  • Paw thermal stimulator G. Ozaki, University of California, USA
  • Compoxmds are tested against uninflamed as well as vehicle treated control groups. Substance application is performed at different time points via different application routes (i.v., i.p., p.o., i.t., i.c.v., s.c, intradermal, transdermal) prior to pain testing.
  • application routes i.v., i.p., p.o., i.t., i.c.v., s.c, intradermal, transdermal
  • Mechanical allodynia is measured by means of a pressure transducer (electronic von Frey Anesthesiometer, HTC Lie-Life Science Instruments, Woodland Hills, SA, USA).
  • Compounds are tested against diabetic and non-diabetic vehicle treated control groups. Substance application is performed at different time points via different application routes (i.v., i.p., p.o., i.t., i.c.v., s.c, intradermal, transdermal) prior to pain testing.
  • application routes i.v., i.p., p.o., i.t., i.c.v., s.c, intradermal, transdermal
  • the compoxmds of the present invention also show excellent selectivity, and strong activity in other assays 2-5 and assays for pain described above.
  • Example 1-1 compounds in Example 1-2 to 1-3 as shown in Table 1 were synthesized.
  • Example 2-4 to 2-9 as shown in Table 2 were synthesized.
  • a mixtare of benzyl [7-(hydroxymethyl)-5,6,7,8-tetrahydronaphthalen-l-yl]carbamate (32.0 mg, 0.10 mmol) and palladium carbon (30 mg) in ethanol (2 ml) was stined under hydrogen at room temperatare for 16 hours.
  • the resulting mixtare was filtered through a pad of celite, and the filfrate was concentrated under reduced pressure.
  • 2,2-Difluoro-l,3-benzodioxole-5-carbonitrile 1000 mg, 5.46 mol
  • ethanol 100 ml
  • Pd/C 200 mg
  • the catalyst is filtered off.
  • the solvent is removed under reduced pressure and the crade mixture is treated with diethyl ether.
  • the resulting crystals are separated from the solvent via a glass filter.
  • Phenyl-[(7R)-7-hydroxy-5,6,7,8-tefrahydronaphthalen-l-yl]carbamate (100 mg, 0.35 mmol) and 1- [2,2-difluoro-l,3-benzodioxol-5-yl]methanamine (66 mg, 0.35 mmol) are dissolved in dimethylsulfoxide (2.00 ml) and stined at room temperatare for lh. The raw material is purified via HPLC.
  • Phenyl-[(7R)-7-hydroxy-5,6,7,8-tefrahydronaphthalen-l-yl]carbamate 300 mg, 1.06 mmol
  • l-[3- chloro-5-(trifluoromethyl)pyridin-2-yl]methanamine hydrochloride 261 mg, 1.06 mmol
  • N, ⁇ - diisopropylethylamine (191 mg, 1.48 mmol) are dissolved in dimethylsulfoxide (2.00 ml).
  • the mixture is reacted at 60 °C for 3h, partitioned between ethyl acetate and water, the organic layer is dried over magnesium sulfate and evaporated to dryness in vacuo.
  • the raw material is triturated with diethyl ether, filtered and dried.
  • Example 1-1 compounds in Example 1-2 to 1- 4 as shown in Table 1 were synthesized.
  • N-Methyl-4-trifluoromethoxyaniline 100 mg, 0.52 mmol
  • ethyl bromoacetate 262 mg, 1.57 mmol
  • sodium carbonate 166 mg, 1.57 mmol
  • the reaction mixtare is partitioned between ethyl acetate and water, the organic layer is dried over magnesium sulfate and evaporated to dryness in vacuo.
  • the raw material is purified by preparative HPLC with an acetonitrile/water gradient.
  • Ethyl- ⁇ -methyl- ⁇ -[4-(trifluoromethoxy)phenyl]glycinate 200 mg, 0.72 mmol
  • potassium hydroxide 81 mg, 1.44 mmol
  • the organic extracts are dried over magnesium sulfate and evaporated to dryness in vacuo.
  • the raw material is purified by preparative chromatography on silica (eluent: ethyl acetate/methanol, 1:0 - 5:1).
  • the compound is obtained accordingly to the procedure for starting compound H from 5-bromo- pyridine-2-carboxylic acid (93 mg, 0.46 mmol) and [4-(frifluoromethyl)phenyl]boronic acid (105 mg, 0.55 mmol).
  • (2R)-8-Amino-l,2,3,4-tefrahydro-naphthalen-2-ol (21 mg, 0.13 mmol), N'-(3-dimethylamino- propyl)-N-ethylcarbodiimide hydrochloride (32 mg, 0.17 mmol), 1 -hydroxy- IH-benzotriazole (21 mg, 0.15 mmol) and N-methyl-N-[4-(trifluoromethoxy)phenyl] glycine (35 mg, 0.14 mmol) are dissolved in dimethylacetamide (3 ml).
  • the reaction mixture is stined over night at room temperature, partitioned between ethyl acetate and water, dried over magnesium sulfate and evaporated to dryness in vacuo.
  • the raw material is purified by chromatography on silica (eluent: cyclohexane/ethyl acetate, 1:1).
  • (2R)-8-amino-l,2,3,4-tefrahydro-naphthalen-2-ol 150 mg, 0.92 mmol
  • N'-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride 229 mg, 1.19 mmol
  • 1-hydroxy-lH-benzotriazole 149 mg, 1.10 mmol
  • (4-chlorophenoxy)acetic acid 189 mg, 1.01 mmol
  • Example 3-8 The compound is obtained accordingly to the procedure for Example 3-8 (omitting the first chromatography over silica gel) from (2R)-8-amino-l,2,3,4-tetrahydro-naphthalen-2-ol (100 mg, 0.61 mmol) and [4-(trifluoromethyl)phenoxy]acetic acid (148 mg, 0.67 mmol).
  • Example 3-8 The compound is obtained accordingly to the procedure for Example 3-8 (the crude reaction mixture is applied to reversed phase HPLC purification directly) from (2R)-8-amino-l, 2,3,4- tefrahydro-naphthalen-2-ol (25 mg, 0.15 mmol) and 5-[4-(trifluoromethoxy)phenyl]pyridine-2- carboxylic acid (50 mg, 0.18 mmol).
  • Example 3-8 The compound is obtained accordingly to the procedure for Example 3-8 (the crude reaction mixtare is applied to reversed phase HPLC purification directly) from (2R)-8-amino-l, 2,3,4- tefrahydro-naphthalen-2-ol (42 mg, 0.26 mmol) and 5-[4-(trifluoromethyl)phenyl]pyridine-2- carboxylic acid (75 mg, 0.28 mmol).
  • Example 3-8 The compound is obtained accordingly to the procedure for Example 3-8 (the crade reaction mixture is applied to reversed phase HPLC purification directly) from (2R)-8-amino-l,2,3,4- tefrahydro-naphthalen-2-ol (117 mg, 0.71 mmol) and 6-[4-(trifluoromethyl)phenyl]nicotinic acid (210 mg, 0.79 mmol).
  • Example 3-8 The compound is obtained accordingly to the procedure for Example 3-8 (the crade reaction mixture is applied to reversed phase HPLC purification directly) from (2R)-8-amino-l,2,3,4- tefrahydro-naphthalen-2-ol (37 mg, 0.23 mmol) and 6-[4-(trifluoromethoxy)phenyl]nicotinic acid (71 mg, 0.25 mmol).
  • a mixture of 4-amino-5,6,7,8-tetrahydroquinolin-6-ol and 4-chloro-3-trifluoromethylphenyl iso- cyanate in tetrahydrofuran is stined at 50°C for 5 hours. After removing the solvent, the resulting residue is purified by silica gel column chromatography to provide N-[4-chloro-3-(trifluoro- methyl)phenyl]-N l -(6-hydroxy-5,6,7,8-tefrahydroquinolin-4-yl)urea.
  • Example 1-2 to 1- 8 as shown in Table 1 are synthesized.
  • Example 2-1 to 2-8 as shown in Table 2 Example 3-1 to 3-8 as shown in Table 3, and Example 4-1 to 4-8 as shown in Table 4 are synthesized in a similar maimer as as described in Example 1-1.

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Abstract

This invention relates to tetrahydro-naphthalene and urea derivatives and salts thereof which are useful as active ingredients of pharmaceutical preparations. The tetrahydro-naphthalene and urea derivatives of the present invention have vanilloid receptor (VR1) antagonistic activity, and can be used for the prophylaxis and treatment of diseases associated with VR1 activity, in particular for the treatment of urological diseases or disorders, such as detrusor overactivity (overactive bladder), urinary incontinence, neurogenic detrusor oeractivity (detrusor hyperflexia), idiopathic detrusor overactivity (detrusor instability), benign prostatic hyperplasia, and lower urinary tract symptoms; chronic pain, neuropathic pain, postoperative pain, rheumatoid arthritic pain, neuralgia, neuropathies, algesia, nerve injury, ischaemia, neurodegeneration, stroke, and inflammatory disorders such as asthma and chronic obstructive pulmonary (or airways) disease (COPD). BHC 03 2 001-Foreign-Countries - 66 - BHC 03 2 001-Foreign-Countries - 65 -

Description

TETRAHYDRO-NAPHTHALENE AND UREA DERIVATIVES
DETAILED DESCRIPTION OF INVENTION
TECHNICAL FIELD
The present invention relates to a tetrahydro-naphthalene or an urea derivative which is useful as an active ingredient of pharmaceutical preparations. The tetrahydro-naphthalene and urea derivatives of the present invention have vanilloid receptor (VR1) antagonistic activity, and can be used for the prophylaxis and treatment of diseases associated with VR1 activity, in particular for the treatment of urological diseases or disorders, such as detrusor overactivity (overactive bladder), urinary incontinence, neurogenic detrusor oeractivity (detrusor hyperflexia), idiopathic detrusor overactivity (detrusor instability), benign prostatic hypeφlasia, and lower urinary tract symptoms; chronic pain, neuropathic pain, postoperative pain, rheumatoid arthritic pain, neuralgia, neuropathies, algesia, nerve injury, ischaemia, neurodegeneration, stroke, and inflammatory disorders such as asthma and chronic obstructive pulmonary (or airways) disease (COPD).
BACKGROUND ART
Vanilloid compounds are characterized by the presence of vanillyl group or a functionally equivalent group. Examples of several vanilloid compounds or vanilloid receptor modulators are vanillin (4-hydroxy-3-methoxy-benzaldehyde), guaiacol (2-methoxy-phenol), zingerone (4-/4-hydroxy-3- methoxyphenyl/-2-butanon), eugenol (2-methoxy4-/2-propenyl/phenol), and capsaicin (8-methy-N- vanillyl-6-noneneamide).
Among others, capsaicin, the main pungent ingredient in "hot" chili peppers, is a specific neurotoxin that desensitizes C-fϊber afferent neurons. Capsaicin interacts with vanilloid receptors (VR1), which are predominantly expressed in cell bodies of dorsal root ganglia (DRG) or nerve endings of afferent sensory fibers including C-fiber nerve endings [Tominaga M, Caterina MJ, Malmberg AB, Rosen TA, Gilbert H, Skinner K, Raumann BE, Basbaum Al, Julius D: The cloned capsaicin receptor integrates multiple pain-producing stimuli. Neuron. 21: 531-543, 1998]. The VR1 receptor was recently cloned [Caterina MJ, Schumacher MA, Tominaga M, Rosen TA, Levine JD, Julius D: Nature 389: 816-824, (1997)] and identified as a nonselective cation channel with six transmembrane domains that is structurally related to the TRP (transient receptor potential) channel family. Binding of capsaicin to VRl allows sodium, calcium and possibly potassium ions to flow down their concentration gradients, causing initial depolarization and release of neurotransmitters from the nerve terminals. VRl can therefore be viewed as a molecular integrator of chemical and physical stimuli that elicit neuronal signals in pathological conditions or diseases.
There is abundant direct or indirect evidence that shows the relation between VRl activity and diseases such as pain, ischaemia, and inflammatory disorders (e.g., WO 99/00115 and 00/50387). Further, it has been demonstrated that VRl transduces reflex signals that are involved in the overactive bladder of patients who have damaged or abnormal spinal reflex pathways [De Groat WC: A neurologic basis for the overactive bladder. Urology 50 (6A Suppl): 36-52, 1997]. Desensitisation of the afferent nerves by depleting neurotransmitters using VRl agonists such as capsaicin has been shown to give promising results in the treatment of bladder dysfunction associated with spinal cord injury and multiple sclerosis [(Maggi CA: Therapeutic potential of capsaicin-like molecules - Studies in animals and humans. Life Sciences 51: 1777-1781, 1992) and (DeRidder D; Chandiramani V; Dasgupta P; VanPoppel H; Baert L; Fowler CJ: Intravesical capsaicin as a treatment for refractory detrusor hypeneflexia: A dual center study with long-term followup. J. Urol. 158: 2087-2092, 1997)].
It is anticipated that antagonism of the VRl receptor would lead to the blockage of neuro- transmitter release, resulting in prophylaxis and treatment of the conditions and diseases associated with VRl activity.
It is therefore expected that antagonists of the VRl receptor can be used for prophylaxis and treatment of the conditions and diseases including chronic pain, neuropathic pain, postoperative pain, rheumatoid arthritic pain, neuralgia, neuropathies, algesia, nerve injury, ischaemia, neuro- degeneration, stroke, inflammatory disorders, urinary incontinence (UI) such as urge urinary incontinence (UUI), and/or overactive bladder.
UI is the involuntary loss of urine. UUI is one of the most common types of UI together with stress urinary incontinence (SUI) which is usually caused by a defect in the urethral closure mechanism. UUI is often associated with neurological disorders or diseases causing neuronal damages such as dementia, Parkinson's disease, multiple sclerosis, stroke and diabetes, although it also occurs in individuals with no such disorders. One of the usual causes of UUI is overactive bladder (OAB) which is a medical condition refening to the symptoms of frequency and urgency derived from abnormal contractions and instability of the detrusor muscle.
There are several medications for urinary incontinence on the market today mainly to help treating UUI. Therapy for OAB is focused on drugs that affect peripheral neural control mechanisms or those that act directly on bladder detrusor smooth muscle contraction, with a major emphasis on development of anticholinergic agents. These agents can inhibit the parasympafhetic nerves which control bladder voiding or can exert a direct spasmolytic effect on the detrusor muscle of the bladder. This results in a decrease in infravesicular pressure, an increase in capacity and a reduction in the frequency of bladder contraction. Orally active anticholinergic drugs which are commonly prescribed, such as propantheline (ProBanthine), tolterodine tartrate (Detrol) and oxybutynin (Difropan), have serious drawbacks such as unacceptable side effects such as dry mouth, abnormal visions, constipation, and central nervous system disturbances. These side effects lead to poor compliance. Dry mouth symptoms alone are responsible for a 70% non-compliance rate with oxybutynin. The inadequacies of present therapies highlight the need for novel, efficacious, safe, orally available drugs that have fewer side effects.
WO03/014064 discloses the compounds represented by the general formula:
Figure imgf000004_0001
wherein
X represents C3-8 cycloalkyl optionally fused by benzene, optionally substituted naphthyl, optionally substituted phenyl, optionally substituted phenyl CI-6 straight alkyl, phenyl fused by cycloalykyl, etc;
Qaa represents CH or N;
Raa represents hydrogen or methyl;
R b represents hydrogen or methyl; and
Y represents substituted naphthyl,
as a vanilloid receptor antagonist.
WO03/022809 discloses the compounds having vanilloid receptor antagonist activity represented by the general formula:
Figure imgf000005_0001
wherein
P and P' independently represent aryl or heteroaryl;
R and R independently represent hydrogen, alkoxy, hydroxy, etc;
n is 0, 1, 2 or 3; p and q are independently 0,1, 2, 3 or 4; r is 1, 2 or 3; and s is 0, 1 or 2.
WO03/053945 discloses the compounds having vanilloid receptor antagonist activity represented by the general formula:
Figure imgf000005_0002
wherein represents phenyl, naphthyl or heterocyclyl;
n is 2, 3, 4, 5 or 6; p is independently 0,1, 2, 3 or 4;
R represents hydrogen, alkoxy, hydroxy, etc; and
R represents
Figure imgf000005_0003
wherein X is a bond, C, O, or NR ; and r, q, R b3 , r R»b4 a.re defined in the application, WO03/070247 discloses the compounds having vanilloid receptor antagonist activity represented by the general formula:
Figure imgf000006_0001
wherein
Xc, represents N or CRcl; Xc2 represents N or CRc2; Xc3 represents N, NRc3 or CRc3; Xc4 represents a bond, N or CR°4; Xc5 represents N or C; provided that at least one of Xci, Xc2, Xc3 and Xc4 is N; Zc_ represents O, NH or S; Zc2 represents a bond, NH or S; Lc represents alkylene, cycloalkylene, etc; Rcl, Rc2, Rc3, Rc4, Rc5, Rc6, Rc7, Rc8a RcSb are defined in the application; and Rc9 represents hydrogen, aryl, cycloalkyl, and heterocylcle.
WO03/080578 discloses the compounds having vanilloid receptor antagonist activity represented by the general formula:
Figure imgf000006_0002
wherein
Ad, Bd, D and Ed are each C or N with the proviso that one or more are N; Xd is an O, S or =NCN; Yα is an aryl, heteroaryl, carbocyclyl or tased-carbocyclyl; n is 0, 1, 2 or 3; and R", R" , R , Ra4,
R and R > d6 are defined in the application.
The development of a compound which has effective VRl antagonistic activity and can be used for the prophylaxis and treatment of diseases associated with VRl activity, in particular for the treatment of urinary incontinence, urge urinary incontinence, overactive bladder as well as pain, and/or inflammatory diseases such as asthma and COPD has been desired. SUMMARY OF THE INVENTION
This invention is to provide a compound of the formula (A), their tautomeric and stereoisomeric form, and salts thereof:
Figure imgf000007_0001
wherein represents the formula
Figure imgf000007_0002
wherein represents the connection position to the molecule and Qi, Q2, Q3 and Q , are defined below,
and represents the formula
Figure imgf000007_0003
wherein
# represents the connection position to the molecule and n, m, p, X, R, R1 and R4 are defined below. CHAPTER I (SUMMARY OF THE INVENTION)
This invention is to provide an urea derivative of the formula (I), their tautomeric and stereo- isomeric form, and salts thereof:
Figure imgf000008_0001
wherein n represents an integer of 0 to 6;
Q] and Q independently represent direct bond or methylene;
Chemical bond between Q2__r__rQ3 is selected from the group consisting of a single bond and a double bond; when Q2.__z_.Q3 is a single bond, Q2 represents CHR2, or CO, and Q3 represents CHR3, when Q, — Q, is a double bond, Q2 represents CR2 and Q3 represents CR3; wherein
R2 represents hydrogen, hydroxy, Cι.6 alkoxy or Cι-6 alkanoyloxy;
R3 represents hydrogen, hydroxy, C1-6 alkoxy, Cι-6 alkanoyloxy, or C1-6 alkyl optionally substituted by hydroxy, C_.6 alkoxy or C__6 alkanoyloxy, with the proviso that Qj and Q can not be direct bond at the same time;
R2 and R3 can not be hydrogen at the same time; when Qi and Q4 are both methylene and R3 is hydroxy, R2 is hydroxy, C__6 alkoxy or Cι-6 alkanoyloxy; when Q. is direct bond, R2 is hydroxy, Cι.6 alkoxy or C_-6 alkanoyloxy; and when Q4 is direct bond, R2 is hydrogen, C_-6 alkoxy or Cj._6 alkanoyloxy; and R4 represents aryl optionally having one or two substitaents selected from the group consisting of halogen, hydroxy, .6 alkylamino, di(Cι-6 alkyl)amino, C3-8 cycloalkylamino, C1-6 alkoxycarbonyl, phenyl, benzyl, sulfonamide, C_-6 alkanoyl, C1-6 alkanoylamino, carbamoyl, Cι-6 alkylcarbamoyl, cyano, C_.6 alkyl optionally substituted by cyano, Cι-6 alkoxycarbonyl, or mono-, di-, or tri-halogen, C..6 alkoxy optionally substitated by mono-, di-, or tri- halogen, phenoxy optionally substitated by halogen or C__6 alkyl, and .6 alkylthio optionally substitated by mono-, di-, or tri- halogen.
In another embodiment, the urea derivatives of formula (I) are those wherein; Qi and Q4 represent methylene;
Q- — Q-, ls a single bond;
Q2 represents CHR2, or CO, wherein
R2 represents hydroxy, Cι-6 alkoxy or C1-6 alkanoyloxy; and Q3 represents CHR3, wherein
R3 represents hydrogen, hydroxy, Cι.6 alkoxy or Cι-6 alkanoyloxy.
In another embodiment, the urea derivatives of formula (I) are those wherein;
Q] represents methylene; Q4 represents direct bond;
Q. — p. js a single bond;
Q2 represents CHR2 or CO, wherein
R2 represents hydrogen, Cι.6 alkoxy or C_.6 alkanoyloxy; and Q3 represents CHR3, wherein
R3 represents hydrogen, hydroxy, C1-6 alkoxy or Cι-6 alkanoyloxy, with the proviso that R2 and R3 can not be hydrogen at the same time.
In another embodiment, the urea derivatives of formula (I) are those wherein; Qi represents direct bond;
Q4 represents methylene; O — 0^ is a single bond; Q2 represents CHR2 or CO, wherein R2 represents hydroxy, Cι-6 alkoxy or Cι_6 alkanoyloxy;
Q3 represents CHR3, wherein
R3 represents hydrogen, hydroxy, Cι_6 alkoxy or C_-6 alkanoyloxy.
In another embodiment, the urea derivatives of formula (T) are those wherein; Qi and Q represent methylene;
Q- — Q- is a double bond; Q2 represents CR2, wherein
R2 represents Cι_6 alkoxy or Cι-6 alkanoyloxy; and Q3 represents CR3, wherein R3 represents hydrogen, Cι-6 alkoxy or C_.6 alkanoyloxy. In another embodiment, the urea derivatives of formula (I) are those wherein;
Qi and Q4 represent methylene;
Q?— -0? is a single bond or a double bond; when Q2r.z_.Q3 is a single bond, Q2 represents CH2 and Q3 represents CHR3, and when Q2_ _rQ3 is a double bond, Q2 represents CH and Q3 represents CR3, wherein
R3 represents C_-6 alkyl optionally substituted by hydroxy.
Preferably, the urea derivatives of formula (I) are those wherein; n represents an integer of 0 to 1; and R4 represents phenyl optionally substitated with one or more substitaents selected from the group consisting of chloro, bro o, fluoro, nitro, mthoxy, trifluoromethyl and trifluoromethoxy.
More preferably, said urea derivative of the formula (I) is selected from the group consisting of:
N-[4-Chloro-3 -(trifluoromethyl)phenyl] -N-(6,7-dihydroxy-5 ,6,7, 8-tetrahydronaphthalen- 1 - yl)urea;
N-[4-Chloro-3-(trifluoromethyl)phenyl]-N'-(7-hydroxy-6-methoxy-5,6,7,8- tetrahydronaphthalen- 1 -yl)urea
N-[4-Chloro-3-(trifluoromethyl)phenyl]-N-(6-hydroxy-7-methoxy-5,6,7,8- tetrahydronaphthalen- 1 -yl)urea; 4-[({[4-Chloro-3-(trifluoromethyl)phenyl]amino}carbonyl)amino]-2,3-dihydro-lH-inden- 2-yl acetate;
4-[({[4-(Trifluoromethyl)benzyl]amino}carbonyl)amino]-2,3-dihydro-lH-inden-2-yl acetate;
N-[4-Chloro-3-(1rifluoromemyl)phenyl]-N-(l-hydroxy-2,3-dihydro-lH-inden-4-yl)urea; N-[4-Chloro-3-(trifluoromethyl)phenyl]-N'-(6-hydroxy-5,6,7,8-tetrahydronaphthalen-l- yl)urea; and N-(6-Hydroxy-5,6,7,8-tefrahydronaphthalen-l-yl)-N'-[4-(trifluoromethyl)benzyl]urea.
CHAPTER B (SUMMARY OF THE INVENTION)
This invention is to provide a hydroxy-tefrahydro-naphthalene derivatives of the formula (I), their tautomeric and stereoisomeric form, and salts thereof:
Figure imgf000012_0001
wherein n represents an integer of 0 to 6; and
R1 represents C3_8cycloalkyl optionally fused by aryl, wherein said aryl is optionally substitated with one or more substitaents selected from the group consisting of halogen, hydroxy, carboxy, nitro, cyano, amino, Cι_6 alkylamino, di(Cι-6 alkyl)amino, C_-6 alkoxycarbonyl, C__6 alkanoyl, Cι-6 alkanoylamino, carbamoyl, Cι-6 alkylcarbamoyl, Cι-6 alkyl optionally substituted by cyano, C_-6 alkoxycarbonyl, or mono-, di-, or tri- halogen, C..6 alkoxy optionally substitated by mono-, di-, or tri- halogen and C].6 alkylthio optionally substitated by mono-, di-, or tri- halogen; phenyl substitated by heteroaryl, or heteroaryloxy, wherein said heteroaryl and heteroaryloxy are optionally substituted with one or more substitaents selected from the group consisting of halogen, hydroxy, carboxy, nitro, cyano, amino, C_.6 alkylamino, di(C_-6 alkyl)amino, C_.6 alkoxycarbonyl, Q.6 alkanoyl, C__6 alkanoylamino, carbamoyl, Cι-6 alkylcarbamoyl, Cι-6 alkyl optionally substituted by cyano, C_.6 alkoxycarbonyl, or mono-, di-, or xri-halogen, C__6 alkoxy optionally substitated by mono-, di-, or tri- halogen, and C1-6 alkylthio optionally substituted by mono-, di-, or tri- halogen; phenyl fused with heteroaryl, or heterocyclyl, wherein said heteroaryl is optionally substitated ■ with one or more substitaents selected from the group consisting of halogen, hydroxy, carboxy, nitro, cyano, amino, C]_6 alkylamino, di(Cι.β alkyl)amino, Cι_6 alkoxycarbonyl, C,-6 alkanoyl, Cι-6 alkanoylamino, carbamoyl, Cι-6 alkylcarbamoyl, Cι_6 alkyl optionally substitated by cyano, C1-6 alkoxycarbonyl, or mono-, di-, or tri-halogen, C1-6 alkoxy optionally substitated by mono-, di-, or tri- halogen and .6 alkylthio optionally substitated by mono-, di-, or tri- halogen; or heteroaryl optionally substitated with one or more substituents selected from the group consisting of halogen, hydroxy, carboxy, nitro, cyano, amino, phenyl, benzyl, C_-6 alkylamino, di(Cι-6 alkyl)amino, C1-6 alkoxycarbonyl, Cχ.6 alkanoyl, Cι_6 alkanoylamino, carbamoyl, C__6 alkylcarbamoyl, Cι-6 alkyl optionally substituted by cyano, C1-6 alkoxycarbonyl, or mono-, di-, or tri-halogen, C_-6 alkoxy optionally substituted by mono-, di-, or tri- halogen and C_.6 alkylthio optionally substitated by mono-, di-, or tri- halogen.
In another embodiment, the hydroxy-tetrahydro-naphthalenylurea derivatives of formula (I) can be those wherein; n represents an integer of 0 or 1 ; and
R1 represents C5-6cycloalkyl optionally fused by benzene, pyridine, or pyrimidine, wherein said benzene, pyridine, and pyrimidine are optionally substitated by halogen, nitro, or Cι-6 alkyl optionally substitated by mono-, di-, or tri-halogen.
In another embodiment, the hydroxy-tefrahydro-naphthalenylurea derivatives of formula (I) can be those wherein; n represents an integer of 0 or 1; and
R1 represents phenyl substituted by thienyl, furyl, pynolyl, thiazolyl, oxazolyl, isoxazolyl, imidazolyl, pyridyl, pyrimidyl, friazolthiadiazolyl, thienyloxy, furyloxy, pynolyl, thiazolyloxy, oxazolyloxy, isoxazolyloxy, imidazolyloxy, pyridyloxy or pyrimidyloxy, wherein said thienyl, furyl, pynolyl, thiazolyl, oxazolyl, isoxazolyl, imidazolyl, pyridyl, pyrimidyl, friazolthiadiazolyl, thienyloxy, furyloxy, pynolyl, thiazolyloxy, oxazolyloxy, isoxazolyloxy, imidazolyloxy, pyridyloxy and pyrimidyloxy are optionally substitated with one or more substituents selected from the group consisting of halogen, nitro, Cι-6 alkyl optionally substituted by mono-, di-, or tri- halogen, Cι_6 alkoxy optionally substituted by mono-, di-, or tri- halogen, and Cι_6 alkylthio optionally substituted by mono-, di-, or tri- halogen.
In another embodiment, the hydroxy-tetrahydro-naphthalenylurea derivatives of formula (T) can be those wherein; n represents an integer of 0 or 1 ; and
R1 represents phenyl fused with thiophene, furan, pyrrole, thiazole, oxazole, isoxazole, imidazole, pyridine, pyrimidine, 1,3-dioxalane, tetrahydrofuran. pynolidine, piperidine, or moφholine. wherein said thiophene, furan, pyrrole, thiazole, oxazole, isoxazole, imidazole, pyridine and pyrimidine are optionally substitated with one or more substitaents selected from the group consisting of halogen, nitro, Cι-6 alkyl optionally substitated by mono-, di-, or tri-halogen, -β alkoxy optionally substitated by mono-, di-, or tri- halogen, and Cι-6 alkylthio optionally substitated by mono-, di-, or tri- halogen.
Preferably, the hydroxy-tetrahydro-naphthalenylurea derivatives of formula (I) can be those wherein; n represents an integer of 0 or 1 ; and
R1 represents phenyl fused with 1 ,3-dioxalane or tetrahydrofuran. In another embodiment, the hydroxy-tetrahydro-naphthalenylurea derivatives of formula (I) can be those wherein; n represents an integer of 0 or 1 ;
R1 represents thienyl, furyl, pynolyl, thiazolyl, oxazolyl, isoxazolyl, imidazolyl, pyridyl or pyrimidyl, wherein said thienyl, furyl, pyrrolyl, thiazolyl, oxazolyl, isoxazolyl, imidazolyl, pyridyl and pyrimidyl are optionally substitated with one or more substituents selected from the group consisting of halogen, nitro, Cι-6 alkyl optionally substitated by mono-, di-, or tri-halogen, .6 alkoxy optionally substitated by mono-, di-, or tri- halogen, and Cι-6 alkylthio optionally substitated by mono-, di-, or tri- halogen.
Preferably, the hydroxy-tefrahydro-naphthalene derivative of formula (I) are those wherein; n represents an integer of 0 or 1 ; and
R1 represents pyridyl or isoxazolyl, wherein said pyridyl and oxazolyl are optionally substitated with one or more substituents selected from the group consisting of halogen, nitro, C_.6 alkyl optionally substitated by mono-, di-, or tri-halogen, C_-6 alkoxy optionally substituted by mono-, di-, or tri- halogen, and C1-6 alkylthio optionally substitated by mono-, di-, or tri- halogen.
More preferably, said hydroxy-tefrahydro-naphthalene derivative of the formula (I) is selected from the group consisting of:
N-(5-tert-butylisoxazol-3-yl)-N'-(7-hydroxy-5,6,7,8-tetrahydronaphthalen-l-yl)urea;
N-(2,3-dihydro-lH-inden-l-yl)-N'-(7-hydroxy-5,6,7,8-tetrahydronaphthalen-l-yl)urea; N-(7-hydroxy-5,6,7,8-tetrahydronaphthalen-l-yl)-N-[4-(pyridin-4-yloxy)phenyl]urea;
N-(7-hydroxy-5,6,7,8-tetrahydronaphthalen-l-yl)-N'-(l,2,3,4-tetrahydronaphthalen-l- yl)urea; N-(7-hydroxy-5,6,7,8-tefrahydronaphthalen-l-yl)-N'-[4-(l,2,3-thiadiazol-4-yl)benzyl]urea;
Ν-(l,3-benzodioxol-5-ylmethyl)-Ν'-(7-hydroxy-5,6,7,8-tetrahydronaphthalen-l-yl)urea;
N-(7-hydroxy-5,6,7,8-tetrahydronaphthalen-l-yl)-N'-(3-pyridin-4-ylphenyl)urea; and
Ν-(7-hydroxy-5,6,7,8-tetrahydronaphthalen-l-yl)-Ν'-{[6-(trifluoromethyl)pyridin-3- yl]methyl}urea.
CHAPTER UI (SUMMARY OF THE INVENTION)
This invention is to provide a hydroxy-tefrahydro-naphthalene derivatives of the formula (I), their tautomeric and stereoisomeric form, and salts thereof:
Figure imgf000016_0001
wherein
R1 represents aryl or heteroaryl, wherein said aryl and heteroaryl are optionally substitated with one or more substitaents selected from the group consisting of halogen, nitro, hydroxy, carboxy, amino, C_-6 alkylamino, di(C__6 alkyl)amino, C3_8 cycloalkylamino, C_.6 alkoxycarbonyl, phenyl (which phenyl is optionally substituted by halogen, trifluoromethyl, trifluoromethoxy, nitro, hydroxy, carboxy, amino, C__6 alkylamino, di(Cj..6 alkyl)amino, C3-8 cycloalkylamino, or Cι-6 alkoxycarbonyl), benzyl (in which phenyl moiety is optionally substitated by halogen, nitro, hydroxy, carboxy, amino, C_.6 alkylamino, di(Cι-6 alkyl), amino, C3-8 cycloalkylamino, or Cι-6 alkoxycarbonyl), heterocycle, sulfonamide, Cι.6 alkanoyl, C_.6 alkanoylamino, carbamoyl, .6 alkylcarbamoyl, cyano, Cι-6 alkyl (which alkyl is optionally substitated by cyano, nitro, hydroxy, carboxy, amino, C1-6 alkoxycarbonyl or mono-, di-, or tri-halogen), Cι.6 alkoxy (which alkoxy is optionally substitated by mono-, di-, or tri- halogen), phenoxy (in which phenyl moiety is optionally substitated by halogen, nitro, hydroxy, carboxy, amino, Cι-6 alkylamino, di(C_-6 alkyl)amino, C3.8 cycloalkylamino, or C_-6 alkoxycarbonyl or Cι-6 alkyl), Cι_6 alkylthio (which alkylthio is optionally substitated by mono-, di-, or tri- halogen),
C_-8 cycloalkyl, and heterocycle;
C1-6 alkyl optionally substitated by R11, OR12, SR12 or N(R12)(R13),
wherein
R11 represents aryl or heteroaryl, wherein said aryl and heteroaryl are optionally substitated with one or more substitaents selected from the group consisting of halogen, nitro, hydroxy, carboxy, amino, Cι-6 alkylamino, di(Cι_6 alkyl)amino, C3-8 cycloalkylamino, Cι-6 alkoxycarbonyl, phenyl (which phenyl is optionally substituted by halogen, nitro, hydroxy, carboxy, amino, Cι-6 alkylamino, di(Cι-6 alkyl)amino, C3_8 cycloalkylamino, or C_-6 alkoxycarbonyl), benzyl (in which phenyl moiety is optionally substitated by halogen, nitro, hydroxy, carboxy, amino, C.-β alkylamino, di(C__6 alkyl), amino, C3-8 cycloalkylamino, or Cι_6 alkoxycarbonyl), heterocycle, sulfonamide, C__6 alkanoyl, Cι_6 alkanoylamino, carbamoyl, Cι-6 alkylcarbamoyl, cyano, C_-6 alkyl (which alkyl is optionally substitated by cyano, nitro, hydroxy, carboxy, amino, Cι-6 alkoxycarbonyl or mono-, di-, or tri-halogen), Cι_6 alkoxy (which alkoxy is optionally substitated by mono-, di-, or tri- halogen), phenoxy (in which phenyl moiety is optionally substitated by halogen, nitro, hydroxy, carboxy, amino, Cι-6 alkylamino, di(Cι-6 alkyl)amino, C3_8 cycloalkylamino, or C_-6 alkoxycarbonyl or C]-6 alkyl), C]_6 alkylthio (which alkylthio is optionally substitated by mono-, di-, or tri- halogen), C3-8 cycloalkyl, and heterocycle;
R12 represents aryl, heteroaryl, or C..6 alkyl optionally substituted by aryl or heteroaryl, wherein said aryl and heteroaryl are optionally substitated with one or more substitaents selected from the group consisting of halogen, nitro, hydroxy, carboxy, amino, Cι_6 alkylamino, di(Cι_6 alkyl)amino, C3-8 cycloalkylamino, C_.6 alkoxycarbonyl, phenyl (which phenyl is optionally substitated by halogen, nitro, hydroxy, carboxy, amino, Cι-6 alkylamino, di(C1-6 alkylamino, C3-8 cycloalkylamino, or C_-6 alkoxycarbonyl), benzyl (in which phenyl moiety is optionally substitated by halogen, nitro, hydroxy, carboxy, amino, Cι-6 alkylamino, di(Cι-6 alkyl), amino, C3-8 cycloalkyl- amino, or .6 alkoxycarbonyl), heterocycle, sulfonamide, Cι_6 alkanoyl, Cι_6 alkanoylamino, carbamoyl, Cι-6 alkylcarbamoyl, cyano, Cι-6 alkyl (which alkyl is optionally substitated by cyano, nitro, hydroxy, carboxy, amino, C1-6 alkoxycarbonyl or mono-, di-, or tri-halogen), Cι_6 alkoxy (which alkoxy is optionally substituted by mono-, di-, or tri- halogen), phenoxy (in which phenyl moiety is optionally substituted by halogen, nitro, hydroxy, carboxy, amino, .6 alkylamino, di(Cι_6 alkyl)amino, C3_8 cycloalkylamino, or Cι.6 alkoxycarbonyl or Cι-6 alkyl), Cι.6 alkylthio (which alkylthio is optionally substitated by mono-, di-, or tri- halogen), C3-8 cycloalkyl, and heterocycle; and R13 represents hydrogen, or C1-6 alkyl;
or
C3-8cycloalkyl optionally fused by aryl,
wherein
said aryl is optionally substitated with one or more substituents selected from the group consisting of halogen, nitro, hydroxy, carboxy, amino, Cι-6 alkylamino, di(C_.6 alkyl)amino,
C3-8 cycloalkylamino, C_-6 alkoxycarbonyl, phenyl (which phenyl is optionally substitated by halogen, nitro, hydroxy, carboxy, amino, C1-6 alkylamino, di(Cι-6 alkyl)amino, C3.8 cycloalkylamino, or C__β alkoxycarbonyl), benzyl (in which phenyl moiety is optionally substitated by halogen, nitro, hydroxy, carboxy, amino, C..6 alkylamino, di(Cι.6 alkyl), amino, C3-8 cycloalkylamino, or Cι-6 alkoxycarbonyl), heterocycle, sulfonamide, C]-6 alkanoyl, Cι-6 alkanoylamino, carbamoyl, C_.6 alkylcarbamoyl, cyano, C)-6 alkyl (which alkyl is optionally substituted by cyano, nitro, hydroxy, carboxy, amino, C .6 alkoxycarbonyl or mono-, di-, or tri-halogen), -β alkoxy (which alkoxy is optionally substitated by mono-, di-, or tri- halogen), phenoxy (in which phenyl moiety is optionally substitated by halogen, nitro, hydroxy, carboxy, amino, C_-6 alkylamino, di(Cι.6 alkyl)amino, C3-8 cycloalkylamino, or C1-6 alkoxycarbonyl or C1-6 alkyl), C_-6 alkylthio (which alkylthio is optionally substitated by mono-, di-, or tri- halogen), C3-8 cycloalkyl, and heterocycle. In another embodiment, the hydroxy-tetrahydro-naphthalenylurea derivatives of formula (I) can be those wherein;
R1 represents phenyl, naphthyl, pyridyl, pyrimidyl, indolyl, benzofuranyl, benzo- thiophenyl, quinolinyl or isoquinolinyl, wherein said phenyl, naphthyl, pyridyl, pyrimidyl, indolyl, benzofuranyl, benzothiophenyl, quinolinyl and isoquinolinyl are optionally substitated with one or more substituents selected from the group consisting of halogen, nitro, hydroxy, carboxy, amino, C__6 alkylamino, di(C1-6 alkyl)amino, C3-8 cycloalkylamino, Cι-6 alkoxycarbonyl, phenyl (which phenyl is optionally substituted by halogen, nitro, hydroxy, carboxy, amino, -β alkylamino, di(C_.6 alkyl)amino, C3.8 cycloalkylamino, or Cι-6 alkoxycarbonyl), benzyl (in which phenyl moiety is optionally substituted by halogen, nitro, hydroxy, carboxy, amino, C]-6 alkylamino, di(C_-6 alkyl), amino, C3.8 cycloalkylamino, or Cι_6 alkoxycarbonyl), heterocycle, sulfon- amide, Cι-6 alkanoyl, C]-6 alkanoylamino, carbamoyl, Cι-6 alkylcarbamoyl, cyano, C1-6 alkyl (which alkyl is optionally substituted by cyano, nitro, hydroxy, carboxy, amino, Cι_6 alkoxycarbonyl or mono-, di-, or tri-halogen), Cι-6 alkoxy (which alkoxy is optionally substitated by mono-, di-, or tri- halogen), phenoxy (in which phenyl moiety is optionally substitated by halogen, nitro, hydroxy, carboxy, amino, C_-6 alkylamino, di(Cι_6 alkyl)amino, C3.8 cycloalkylamino, or C__6 alkoxycarbonyl or Cι-6 alkyl), C]-6 alkylthio (which alkylthio is optionally substitated by mono-, di-, or tri- halogen), C3.8 cycloalkyl, and heterocycle.
In another embodiment, the hydroxy-tetrahydro-naphthalenylurea derivatives of formula (I) can be those wherein; R1 represents phenyl, pyridyl, or pyrimidyl, wherein said phenyl, pyridyl, and pyrimidyl are optionally substitated by one or more of substitaents selected from the group consisting of halogen, nitro, C_-6 alkyl (which alkyl is optionally substitated by cyano, nitro, or mono-, di-, or tri-halogen), and Cι-6 alkoxy optionally substitated by mono-, di-, or tri- halogen.
In another embodiment, the hydroxy-tetrahydro-naphthalenylurea derivatives of formula (I) can be those wherein; represents C1-6 alkyl optionally substitated by Rn, OR12, SR12 or N(R12)(R13),
wherein
R11 represents phenyl, naphthyl, pyridyl or pyrimidyl, wherein said phenyl, naphthyl, pyridyl and pyrimidyl are optionally substitated with one or more substitaents selected from the group consisting of halogen, nitro, hydroxy, carboxy, amino, C_-6 alkylamino, di(Cι-6 alkylamino, C3_s cycloalkylamino, Cι_6 alkoxycarbonyl, benzyl (in which phenyl moiety is optionally substitated by halogen, nitro, hydroxy, carboxy, amino, Cι-6 alkylamino, di(C_-6 alkyl), amino, C3.8 cycloalkylamino, or C_-6 alkoxycarbonyl), heterocycle, sulfonamide, Cι-6 alkanoyl, .6 alkanoylamino, carbamoyl, C_-6 alkylcarbamoyl, cyano, C_-6 alkyl (which alkyl is optionally substitated by cyano, nitro, hydroxy, carboxy, amino, C_-6 alkoxycarbonyl or mono-, di-, or tri-halogen), C..6 alkoxy (which alkoxy is optionally substituted by mono-, di-, or tri- halogen), phenoxy (in which phenyl moiety is optionally substituted by halogen, nitro, hydroxy, carboxy, amino, C__6 alkylamino, di(Cι.6 alkyl)amino, C3_8 cycloalkylamino, or Cj.6 alkoxycarbonyl or Cι_6 alkyl), _6 alkylthio (which alkylthio is optionally substituted by mono-, di-, or tri- halogen), C3-8 cycloalkyl, and heterocycle;
R12 represents pheny, naphthyl, pyridyl, pyrimidyl, or Cι_6 alkyl optionally substituted by phenyl, naphthyl, pyridyl or pyrimidyl, wherein said phenyl, naphthyl, pyridyl and pyrimidyl are optionally substitated with one or more substituents selected from the group consisting of halogen, nitro, hydroxy, carboxy, amino, C1-6 alkylamino, di(C_-6 alkylamino, C3-8 cycloalkylamino, C_.6 alkoxycarbonyl, phenyl (which phenyl is optionally substituted by halogen, nitro, hydroxy, carboxy, amino, C_-6 alkylamino, di(C_-6 alkyl)amino, C3-8 cycloalkylamino, or C1-6 alkoxy- carbonyl), benzyl (in which phenyl moiety is optionally substituted by halogen, nitro, hydroxy, carboxy, amino, C_-6 alkylamino, di(Cι-6 alkyl), amino, C3_8 cycloalkylamino, or Cι-6 alkoxycarbonyl), heterocycle, sulfon- amide, C_.6 alkanoyl, Cι_6 alkanoylamino, carbamoyl, Cι_6 alkylcarbamoyl, cyano, C1-6 alkyl (which alkyl is optionally substitated by cyano, nitro, hydroxy, carboxy, amino, C_.6 alkoxycarbonyl or mono-, di-, or tri- halogen), Cι-6 alkoxy (which alkoxy is optionally substitated by mono-, di-, or tri- halogen), phenoxy (in which phenyl moiety is optionally substitated by halogen, nitro, hydroxy, carboxy, amino, C1-6 alkylamino, di(C1-6 alkyl)amino, C3-8 cycloalkylamino, or Cj..6 alkoxycarbonyl or C1-6 alkyl), Cι_6 alkylthio (which alkylthio is optionally substitated by mono-, di-, or tri- halogen), C3.8 cycloalkyl, and heterocycle; and R13 represents hydrogen, or Cι-6 alkyl.
Preferably, the hydroxy-tetrahydro-naphthalenylurea derivatives of formula (I) can be those wherein;
R1 represents C_. alkyl optionally substitated by phenyl (which phenyl is optionally substitated with one or more substitaents selected from the group consisting of halogen, nitro, Cj-6 alkyl optionally substitated by cyano, C1-6 alkoxycarbonyl or mono-, di-, or tri-halogen, and Cι-6 alkoxy optionally substitated by mono-, di-, or tri- halogen), or N(R12)(R13),
R12 represents phenyl or C_-2 alkyl optionally substitated by phenyl, wherein said phenyl is optionally substitated with one or more substitaents selected from the group consisting of halogen, nitro, Cι_6 alkyl optionally substituted by mono-, di-, or tri-halogen, and Cj.6 alkoxy optionally substitated by mono-, di-, or tri- halogen; and
R13 represents hydrogen, or C_.6 alkyl.
In another embodiment, the hydroxy-tefrahydro-naphthalene derivative of formula (I) are those wherein;
R1 represents C3.8cycloalkyl optionally fused by phenyl, wherein said phenyl is optionally substitated with one or more substitaents selected from 'the group consisting of halogen, nitro, Cι.6 alkyl optionally substituted by mono-, di-, or tri-halogen, and C .6 alkoxy optionally substitated by mono-, di-, or tri- halogen.
More preferably, said hydroxy-tefrahydro-naphthalene derivative of the formula (I) is selected from the group consisting of: N-(7-hydroxy-5,6,7,8-tetrahydronaphthalen-l-yl)-2-methoxybenzamide;
N-(7-hydroxy-5,6,7,8-tetrahydronaphthalen-l-yl)-4-(trifluoromethyl)benzamide;
5-chloro-Ν-(7-hydroxy-5,6,7,8-tefrahydronaphthalen-l-yl)-lH-indole-2-carboxamide;
2-(3-bromophenyl)-N-(7-hydroxy-5,6,7,8-tetrahydronaphthalen-l-yl)acetamide; and
N2-[4-chloro-3-(trifluoromethyl)phenyl]-Nl-(7-hydroxy-5,6,7,8-tetrahydronaphthalen-l- yl)glycinamide.
CHAPTER IV (SUMMARY OF THE INVENTION)
This invention is to provide an urea derivatives of the formula (I), their tautomeric and stereoisomeric form, and salts thereof:
Figure imgf000022_0001
wherein m represents 0, 1, 2, or 3; p represents 0 or 1;
-X- represents a bond, -O- or -NQR.1)- (wherein R1 is hydrogen or C__6 allcyl); with the proviso that when m is 0, -X- represents a bond. Qi, Q2 and Q3 independently represent Ν or CH, with the proviso that at least one of Q_, Q2 and Q3 is Ν; R represents aryl or heteroaryl, wherein said aryl and heteroaryl are optionally substitated with one or more substituents independently selected from the group consisting of halogen, nitro, hydroxy, carboxy, amino, Cι-6 alkylamino, di(Cι-6 alkyl)amino, C3.8 cycloalkyl- amino, C_.6 alkoxycarbonyl, phenyl (which phenyl is optionally substitated by halogen, nitro, hydroxy, carboxy, amino, Cι-6 alkylamino, di(C__6 alkyl)amino, C3_8 cycloalkylamino, or .6 alkoxycarbonyl), benzyl (in which phenyl moiety is optionally substitated by halogen, nitro, hydroxy, carboxy, amino, .6 alkylamino, di(C_.6 alkyl)amino, C3-8 cycloalkylamino, or Cι-6 alkoxycarbonyl), sulfonamide, Cι_6 alkanoyl, .6 alkanoylamino, carbamoyl, C__6 alkylcarbamoyl, cyano, C.-6 alkyl (which alkyl is optionally substitated by cyano, nitro, hydroxy, carboxy, amino, Ci.6 alkoxycarbonyl or mono-, di-, or tri-halogen), Cι-6 alkoxy (which alkoxy is optionally substituted by mono-, di-, or tri- halogen), phenoxy (in which phenyl moiety is optionally substitated by halogen, nitro, hydroxy, carboxy, amino, Cι.6 alkylamino, di(Cι-6 alkyl)amino, C3.8 cycloalkylamino, or Cι-6 alkoxycarbonyl or Cι_6 alkyl), C__6 alkylthio (which alkylthio is optionally substitated by mono-, di-, or tri- halogen), C3-8 cycloalkyl, and heterocycle. er embodiment, the urea derivatives of formula (I) can be those wherein;
m represents 0, 1, 2, or 3 ;
p represents 0 or 1;
-X- represents a bond, -O- or -N^1)- (wherein R1 is hydrogen or Cι-6 alkyl); with the proviso that when m is 0, -X- represents a bond.
Qi, Q2 and Q3 independently represent N or CH, with the proviso that at least one of Q_, Q2 and Q3 is N;
R represents phenyl, naphthyl, pyridyl, or pyrimidyl, wherein said phenyl, naphthyl, pyridyl and pyrimidyl are optionally substituted with one or more substituents independently selected from the group consisting of halogen, nitro, hydroxy, carboxy, amino, .6 alkylamino, di(C1-6 alkyl)amino, C3-8 cyclo- alkylamino, Cι-6 alkoxycarbonyl, phenyl (which phenyl is optionally substituted by halogen, nitro, hydroxy, carboxy, amino, Cι-6 alkylamino, di(Cι-6 alkyl)amino, C3-8 cycloalkylamino, or Cι-6 alkoxycarbonyl), benzyl (in which phenyl moiety is optionally substitated by halogen, nitro, hydroxy, carboxy, amino, Cι-6 alkylamino, di(C1-6 alkyl)amino, C3-8 cycloalkylamino, or C1-6 alkoxycarbonyl), sulfonamide, Cj-6 alkanoyl, Cι_6 alkanoylamino, carbamoyl, Cι-6 alkylcarbamoyl, cyano, Cι-6 alkyl (which alkyl is optionally substitated by cyano, nitro, hydroxy, carboxy, amino, Cι_6 alkoxycarbonyl or mono-, di-, or tri-halogen), Cι-6 alkoxy (which alkoxy is optionally substituted by mono-, di-, or tri- halogen), phenoxy (in which phenyl moiety is optionally substitated by halogen, nifro, hydroxy, carboxy, amino, -β alkylamino, di(C__6 alkyl)amino, C3-8 cycloalkylamino, or Cι.6 alkoxycarbonyl or C .6 alkyl), C1-6 alkylthio (which alkylthio is optionally substitated by mono-, di-, or tri- halogen), C3-8 cycloalkyl, and heterocycle.
In another embodiment, the urea derivatives of formula (I) can be those wherein; m represents 0, 1, 2, or 3 ; p represents 0 or 1;
-X- represents a bond, -O- or -N(R])- (wherein R1 is hydrogen or Cι-6 alkyl); with the proviso that when m is 0, -X- represents a bond.
Qi represents N;
Q2 represents CH; Q3 represents CH;
R represents phenyl, naphthyl, pyridyl, or pyrimidyl, wherein said phenyl, naphthyl, pyridyl and pyrimidyl are optionally substitated with one or more substitaents independently selected from the group consisting of halogen, nitro, hydroxy, carboxy, amino, Cι-6 alkylamino, di(Cι-6 alkyl)amino, C3.8 cycloalkylamino, C__6 alkoxycarbonyl, phenyl (which phenyl is optionally substituted by halogen, nitro, hydroxy, carboxy, amino, .6 alkylamino, di(Cι_6 alkyl)amino, C3_8 cycloalkylamino, or .6 alkoxycarbonyl), benzyl (in which phenyl moiety is optionally substituted by halogen, nifro, hydroxy, carboxy, amino, C_-6 alkylamino, di(Cι-6 alkyl)amino, C3_8 cycloalkylamino, or C_-6 alkoxycarbonyl), sulfonamide, . Cι-6 alkanoyl, C1-6 alkanoylamino, carbamoyl, Cι-6 alkylcarbamoyl, cyano, Cι_6 alkyl (which alkyl is optionally substituted by cyano, nitro, hydroxy, carboxy, amino, Cι-6 alkoxycarbonyl or mono-, di-, or tri-halogen), Cι_6 alkoxy (which alkoxy is optionally substitated by mono-, di-, or tri- halogen), phenoxy (in which phenyl moiety is optionally substitated by halogen, nifro, hydroxy, carboxy, amino, C__6 alkylamino, di(C!.6 alkyl)amino, C3-8 cycloalkylamino, or Cι-6 alkoxycarbonyl or Cι_6 alkyl), Cι-6 alkylthio (which alkylthio is optionally substituted by mono-, di-, or tri- halogen), C3_8 cycloalkyl, and heterocycle.
In another embodiment, the urea derivatives of formula (I) can be those wherein; m represents 0, 1, 2, or 3; p represents 0 or 1;
-X- represents a bond, -O- or -N(R')- (wherein R1 is hydrogen or Cι-6 allcyl); with the proviso that when m is 0, -X- represents a bond.
Qi represents CH; Q2 represents CH;
Q3 represents N;
R represents phenyl, naphthyl, pyridyl, or pyrimidyl, wherein said phenyl, naphthyl, pyridyl and pyrimidyl are optionally substitated with one or more substituents independently selected from the group consisting of halogen, nitro, hydroxy, carboxy, amino, Cι_6 alkylamino, di(Cι.6 alkyl)amino, C3-8 cycloalkylamino, Ci.6 alkoxycarbonyl, phenyl (which phenyl is optionally substituted by halogen, nitro, hydroxy, carboxy, amino, Cι-6 alkylamino, di(Cι.6 alkyl)amino, C3.8 cycloalkylamino, or Cι-6 alkoxycarbonyl), benzyl (in which phenyl moiety is optionally substituted by halogen, nitro, hydroxy, carboxy, amino, C..6 alkylamino, di(C].6 alkyl)amino, C3-8 cycloalkylamino, or C..6 alkoxycarbonyl), sulfonamide, C1-6 alkanoyl, Cι-6 alkanoylamino, carbamoyl, C_-6 alkylcarbamoyl, cyano, Cι-6 alkyl (which alkyl is optionally substitated by cyano, nitro, hydroxy, carboxy, amino, Cι-6 alkoxycarbonyl or mono-, di-, or tri-halogen), Cι_6 alkoxy (which alkoxy is optionally substitated by mono-, di-, or tri- halogen), phenoxy (in which phenyl moiety is optionally substituted by halogen, nitro, hydroxy, carboxy, amino, C__6 alkylamino, di(C_-5 alkyl)amino, C3-8 cycloalkylamino, or C)-6 alkoxycarbonyl or Cι-6 alkyl), Cι-6 alkylthio (which alkylthio is optionally substitated by mono-, di-, or tri- halogen), C3-8 cycloalkyl, and heterocycle. er embodiment, the urea derivatives of formula (I) can be those wherein;
m represents 0, 1, 2, or 3 ;
p represents 0 or 1;
-X- represents a bond, -O- or -N(R')- (wherein R1 is hydrogen or Cι-6 alkyl); with the proviso that when m is 0, -X- represents a bond.
Qi represents CH;
Q2 represents N;
Q3 represents CH;
R represents phenyl, naphthyl, pyridyl, or pyrimidyl, wherein said phenyl, naphthyl, pyridyl and pyrimidyl are optionally substitated with one or more substitaents independently selected from the group consisting of halogen, nitro, hydroxy, carboxy, amino, C1-6 alkylamino, di(Cι-6 alkyl)amino, C3-8 cycloalkylamino, Cι-6 alkoxycarbonyl, phenyl (which phenyl is optionally substitated by halogen, nitro, hydroxy, carboxy, amino, Cj.6 alkylamino, di(C.-6 alkyl)amino, C3.8 cycloalkylamino, or C1-6 alkoxycarbonyl), benzyl (in which phenyl moiety is optionally substitated by halogen, nitro, hydroxy, carboxy, amino, C__6 alkylamino, di(C1-6 alkyl)amino, C3-8 cycloalkylamino, or Cι-6 alkoxycarbonyl), sulfonamide, Cι_6 alkanoyl, C1-6 alkanoylamino, carbamoyl, .6 alkylcarbamoyl, cyano, C__6 alkyl (which alkyl is optionally substituted by cyano, nitro, hydroxy, carboxy, amino, Cι-6 alkoxycarbonyl or mono-, di-, or tri-halogen), C]-6 alkoxy (which alkoxy is optionally substitated by mono-, di-, or tri- halogen), phenoxy (in which phenyl moiety is optionally substitated by halogen, nitro, hydroxy, carboxy, amino, Cι-6 alkylamino, di(Cι-6 alkyl)amino, C3-8 cycloalkylamino, or C1-6 alkoxycarbonyl or C.-6 allcyl), Cι-6 alkylthio (which alkylthio is optionally substitated by mono-, di-, or tri- halogen), C3-8 cycloalkyl, and heterocycle.
In another embodiment, the urea derivatives of formula (I) can be those wherein; m represents 0, 1, 2, or 3 ; p represents 0 or 1;
-X- represents a bond, -O- or -N^1)- (wherein R1 is hydrogen or C__6 allcyl); with the proviso that when m is 0, -X- represents a bond.
Qj represents Ν;
Q2 represents CH; Q3 represents Ν;
R represents phenyl, naphthyl, pyridyl, or pyrimidyl, wherein said phenyl, naphthyl, pyridyl and pyrimidyl are optionally substituted with one or more substituents independently selected from the group consisting of halogen, nitro, hydroxy, carboxy, amino, Cj.6 alkylamino, di(C_.6 alkyl)amino, C3-8 cycloalkylamino, C^ alkoxycarbonyl, phenyl (which phenyl is optionally substitated by halogen, nitro, hydroxy, carboxy, amino, Cι-6 alkylamino, di(Cι-6 alkyl)amino, C3-8 cycloalkylamino, or .6 alkoxycarbonyl), benzyl (in which phenyl moiety is optionally substitated by halogen, nitro, hydroxy, carboxy, amino, Cι.6 alkylamino, di(Cι_6 alkyl)amino, C3-8 cycloalkylamino, or C]-6 alkoxycarbonyl), sulfonamide, Cι-6 alkanoyl, Cι_6 alkanoylamino, carbamoyl, Cι-6 alkylcarbamoyl, cyano, Cι-6 alkyl (which alkyl is optionally substitated by cyano, nitro, hydroxy, carboxy, amino, .6 alkoxycarbonyl or mono-, di-, or tri-halogen), Ci.6 alkoxy (which alkoxy is optionally substituted by mono-, di-, or tri- halogen), phenoxy (in which phenyl moiety is optionally substituted by halogen, nitro, hydroxy, carboxy, amino, Cι.β alkylamino, di(Cι_6 alkyl)amino, C3-8 cycloalkylamino, or Cι_6 alkoxycarbonyl or Cι-6 alkyl), C_-6 alkylthio (which alkylthio is optionally substituted by mono-, di-, or tri- halogen), C3-8 cycloalkyl, and heterocycle. Preferably, the urea derivative of formula (I) are those wherein; m represents 0; p represents 0 or 1;
-X- represents a bond; Q_, Q2 and Q3 independently represent N or CH, with the proviso that at least one of Qb Q2 and Q3 is N; R represents phenyl, naphthyl, pyridyl, or pyrimidyl, wherein said phenyl, naphthyl, pyridyl and pyrimidyl are optionally substituted with one or more substitaents independently selected from the group consisting of halogen, nitro, hydroxy, carboxy, amino, C1-6 alkylamino, di(Cι.6 alkyl)amino, C3-8 cycloalkylamino, .6 alkoxycarbonyl, phenyl (which phenyl is optionally substitated by halogen, nitro, hydroxy, carboxy, amino, Cι-6 alkylamino, di(C1_6 alkyl)amino, C3_8 cycloalkylamino, or Cι-6 alkoxycarbonyl), benzyl (in which phenyl moiety is optionally substituted by halogen, nitro, hydroxy, carboxy, amino, Cι_6 alkylamino, di(C]-6 alkyl)amino, C3-8 cycloalkylamino, or C1-6 alkoxycarbonyl), sulfonamide, C]-6 alkanoyl, Cι-6 alkanoylamino, carbamoyl, Cι-6 alkylcarbamoyl, cyano, C__6 alkyl (which alkyl is optionally substitated by cyano, nitro, hydroxy, carboxy, amino, Cι-6 alkoxycarbonyl or mono-, di-, or tri-halogen), Cι_6 alkoxy (which alkoxy is optionally substituted by mono-, di-, or tri- halogen), phenoxy (in which phenyl moiety is optionally substituted by halogen, nitro, hydroxy, carboxy, amino, C_.6 alkylamino, di(Cι-6 alkyl)amino, C3-8 cycloalkylamino, or Ci.6 alkoxycarbonyl or C_-6 alkyl), C_-6 alkylthio (which alkylthio is optionally substituted by mono-, di-, or tri- halogen), C3_8 cycloalkyl, and heterocycle.
Preferably, the urea derivative of formula (T) are those wherein; m represents 0, 1, 2, or 3 ; p represents 0 or 1;
-X- represents a bond, -O- or -N(R')- (wherein R1 is hydrogen or Cι_6 alkyl); with the proviso that when m is 0, -X- represents a bond. Qi, Q2 and Q3 independently represent N or CH, with the proviso that at least one of Qi, Q2 and Q3 is N;
R represents phenyl, naphthyl, pyridyl, or pyrimidyl, wherein said phenyl, naphthyl, pyridyl, or pyrimidyl is optionally substitated by one or more of substituents selected from the group consisting of chloro, bromo, fluoro, nitro, methoxy, trifluoromethyl, trifluoromethoxy and C_.β alkanoylamino.
More preferably, said urea derivative of the formula (I) is selected from the group consisting of:
N-[4-chloro-3-(xrifluoromethyl)phenyl]-N-(6-hydroxy-5,6,7,8-tetrahydroquinolin-4-yl)urea;
N-(6-hydroxy-5 ,6,7, 8-tetrahydroquinolin-4-yl)-N'-[4-(xrifluoromethyl)benzyl]urea;
N-biphenyl-3-yl-N'-(6-hydroxy-5,6,7,8-tetrahydroquinolin-4-yl)urea;
N-[4-chloro-3-(1rifluoromethyl)phenyl]-N'-(7-hydroxy-5,6,7,8-tetrahydroisoquinolin-l-yl)urea;
N-(7-hydroxy-5,6,7,8-tefrahydroisoquinolin-l-yl)-N-[4-(trifluoromethyl)benzyl]urea;
N-biphenyl-3-yl-N'-(7-hydroxy-5,6,7,8-tetrahydroisoquinolin-l-yl)urea;
N-[4-chloro-3-(trifluoromethyl)phenyl]-N'-(6-hydroxy-5,6,7,8-tetrahydroisoquinolin-4-yl)urea;
N-(6-hydroxy-5,6,7,8-tetrahydroisoquinolin-4-yl)-N-[4-(trifluoromethyl)benzyl]urea;
N-biphenyl-3-yl-N-(6-hydroxy-5,6,7,8-tefrahydroisoquinolin-4-yl)urea;
Ν-[4-chloro-3-(trifluoromethyl)phenyl]-Ν'-(6-hydroxy-5,6,7,8-tetrahydroquinazolin-4-yl)urea;
N-(6-hydroxy-5,6,7,8-tefrahyc_roquinazolin-4-yl)-N'-[4-(trifluoromethyl)benzyl]urea; and
N-biphenyl-3-yl-N'-(6-hydroxy-5,6,7,8-tetrahydroquinazolin-4-yl)urea.
DEFINITIONS
The compounds of the present invention, their tautomeric and stereoisomeric form, and salts thereof sm risingly show excellent VRl antagonistic activity. They are, therefore suitable especially for the prophylaxis and freatment of diseases associated with VRl activity, in particular for the treatment of . urological diseases or disorders, such as detrusor overactivity (overactive bladder), urinary incontinence, neurogenic detrusor oeractivity (detrusor hyperflexia), idiopathic detrusor overactivity (detrusor instability), benign prostatic hypeφlasia, and lower urinary tract symptoms.
The compounds of the present invention are also effective for treating or preventing a disease selected from the group consisting of chronic pain, neuropathic pain, postoperative pain, rheumatoid arthritic pain, neuralgia, neuropathies, algesia, nerve injury, ischaemia, neuro- degeneration and/or stroke, as well as inflammatory diseases such as asthma and COPD since the diseases also relate to VRl activity.
The compounds of the present invention are also useful for the treatment and prophylaxis of neuropathic pain, which is a form of pain often associated with heφes zoster and post-heφetic neuralgia, painful diabetic neuropathy, neuropathic low back pain, postfraumatic and postoperative neuralgia, neuralgia due to nerve compression and other neuralgias, phantom pain, complex regional pain syndromes, infectious or parainfectious neuropathies like those associated with HTV infection, pain associated with central nervous system disorders like multiple sclerosis or Parkinson disease or spinal cord injury or traumatic brain injury, and post-stroke pain.
Furthermore, the compounds of the present invention are useful for the freatment of musculoskeletal pain, forms of pain often associated with osteoarthritis or rheumatoid arthritis or other forms of arthritis, and back pain.
In addition, the compounds of the present invention are useful for the treatment of pain associated with cancer, including visceral or neuropathic pain associated with cancer or cancer treatment.
The compounds of the present invention are furthermore useful for the treatment of visceral pain, e.g. pain associated with obstruction of hollow viscus like gallstone colik, pain associated with irritable bowel syndrome, pelvic pain, vulvodynia, orchialgia or prostatodynia, pain associated with inflammatory lesions of joints, skin, muscles or nerves, and orofascial pain and headache, e.g. migraine or tension-type headache.
Further, the present invention provides a medicament, which includes one of the compounds, described above and optionally pharmaceutically acceptable excipients.
Alkyl per se and "alk" and "alkyl" in alkenyl, alkynyl, alkoxy, alkanoyl, alkylamino, alkylamino- carbonyl, alkylaminosulfonyl, alkylsulfonylamino, alkoxycarbonyl, alkoxycarbonylamino and alkanoylamino represent a linear or branched alkyl radical having generally 1 to 6, preferably 1 to 4 and particularly preferably 1 to 3 carbon atoms, representing illustratively and preferably methyl, ethyl, n-propyl, isopropyl, tert-butyl, n-pentyl and n-hexyl. Alkoxy illustratively and preferably represents methoxy, ethoxy, n-propoxy, isopropoxy, tert- butoxy, n-pentoxy and n-hexoxy.
Alkylamino illustratively and preferably represents an alkylamino radical having one or two (independently selected) allcyl substituents, illustratively and preferably representing methylamino, ethylamino, n-propylamino, isopropylamino, tert-butylamino, n-pentylamino, n-hexylamino, N,N- dimethylamino, NΝ-diethylamino, Ν-ethyl-Ν-methylamino, N-methyl-Ν-n-propylamino, N- isopropyl-Ν-n-propylamino, Ν-t-butyl-N-methylamino, N-ethyl-N-n-pentylamino and N-n-hexyl- Ν-methylamino.
Aryl per se and in arylamino and in arylcarbonyl represents a mono- to tricyclic aromatic carbocyclic radical having generally 6 to 14 carbon atoms, illustratively and preferably representing phenyl, naphthyl and phenanthrenyl.
Cycloalkyl per se and in cycloalkylamino and in cycloalkylcarbonyl represents a cycloalkyl group having generally 3 to 8 and preferably 5 to 7 carbon atoms, illustratively and preferably representing cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl and cycloheptyl.
Heteroaryl per se and the heteroaryl portion of the heteroaralkyl, heteroaryloxy, heteroaralkyloxy, or heteroarylcarbamoyl represent an aromatic mono- or bicyclic radical having generally 5 to 10 and preferably 5 or 6 ring atoms and up to 5 and preferably up to 4 hetero atoms selected from the group consisting of S, O and N, illustratively and preferably representing thienyl, furyl, pynolyl, thiazolyl, oxazolyl, imidazolyl, pyridyl, pyrimidyl, pyridazinyl, indolyl, isoindolino, indazolyl, benzofuranyl, benzothiophenyl, quinolinyl, isoquinolinyl, tetrazolyl, and triazolyl.
Heterocyclyl per se and in heterocyclylcarbonyl represents a mono- or polycyclic, preferably mono- or bicyclic, nonaromatic heterocyclic radical having generally 4 to 10 and preferably 5 to 8 ring atoms and up to 3 and preferably up to 2 hetero atoms and/or hetero groups selected from the group consisting of Ν, O, S, SO and S02. The heterocyclyl radicals can be saturated or partially unsatarated. Preference is given to 5- to 8-membered monocyclic saturated heterocyclyl radicals having up to two hetero atoms selected from the group consisting of O, Ν and S, such as illusfratively and preferably 1,3-dioxalanyl, tefrahydrofuran-2-yl, pynolidin-2-yl, pynolidin-3-yl, pynolinyl, piperidinyl, moφholinyl, perhydroazepinyl. CHAPTER I (EMBODIMENT OF THE INVENTION)
The compound of the formula (I) of the present invention can be, but not limited to be, prepared by combining various known methods. In some embodiments, one or more of the substitaents, such as amino group, carboxyl group, and hydroxyl group of the compounds used as starting materials or intermediates are advantageously protected by a protecting group known to those skilled in the art. Examples of the protecting groups are described in "Protective Groups in Organic Synthesis (3rd Edition)" by Greene and Wuts, John Wiley and Sons, New York 1999.
The compound of the formula (T) of the present invention can be, but not limited to be, prepared by the Method [A], [B], [C], [D], [E] or [F] below.
[Method A]
Figure imgf000032_0001
(ID (III) (I)
The compound of the formula (I) (wherein n, Q_, Q , Q3, Q and R4 are the same as defined above) can be prepared by the reaction of the compound of the formula (II) (wherein Qj, Q2, Q3 and Q are the same as defined above) and the compound of the formula (HI) (wherein n and R4 are the same as defined above).
The reaction may be carried out in a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane; ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; aromatic hydrocarbons such as benzene, toluene and xylene; nitriles such as acetonitrile; amides such as N, N- dimethylformamide (DMF), Ν, Ν-dimethylacetamide (DMAC) and Ν-methylpynolidone (NMP); urea such as l,3-dimethyl-2-imidazolidinone (DMI); sulfoxides such as dimethylsulfoxide (DMSO); and others. Optionally, two or more of the solvents selected from the listed above can be mixed and used.
The reaction can be carried out in the presence of organic base such as pyridine or triethylamine. The reaction temperature can be optionally set depending on the compounds to be reacted. The reaction temperatare is usually, but not limited to, about room temperatare to 100°C. The reaction may be conducted for, usually, 30 minutes to 48 hours and preferably 1 to 24 hours.
The compound (II) and (HI) can be prepared by the use of known techniques or are commercially available.
[Method B]
Figure imgf000033_0001
The compound of the formula (I) (wherein n, Qi, Q2, Q3, Q4 and R are the same as defined above) can be prepared by reacting the compound of the formula (IT) (wherein Q_, Q2, Q3 and Q4 are the same as defined above) with phosgene, diphosgene, triphosgene, 1,1-carbonyldiimidazole (CDI), or l,r-carbonyldi(l,2,4-triazole)(CDT), and then adding the compound of the formula (IV) (wherein n and R4 are the same as defined above) to the reaction mixture.
The reaction may be carried out in a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane; ethers such as diethyl ether, iso- propyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; aromatic hydrocarbons such as benzene, toluene and xylene; nitriles such as acetonitrile; amides such as N, N- dimethylformamide (DMF), N, N-dimefhylacetamide (DMAC) and N-methylpynolidone (NMP); urea such as l,3-dimethyl-2-imidazolidinone (DMI); sulfoxides such as dimethylsulfoxide (DMSO); and others. Optionally, two or more of the solvents selected from the listed above can be mixed and used.
The reaction temperatare can be optionally set depending on the compounds to be reacted. The reaction temperatare is usually, but not limited to, about 20°C to 50°C. The reaction may be conducted for, usually, 30 minutes to 10 hours and preferably 1 to 24 hours.
The compound (IV) is commercially available or can be prepared by the use of known techniques and phosgene, diphosgene, triphosgene, CDI, and CDT are commercially available and . [Method C]
Figure imgf000034_0001
The compound of the formula (I) (wherein n, Qls Q2, Q3, Q and R4 are the same as defined above) can be prepared by reacting the compound of the formula (IT) (wherein Q., Q2, Q3 and Q4 are the same as defined above) with the compound of the formula (V) (wherein L_ represents halogen atom such as chlorine, bromine, or iodine atom) and then adding the compound of the formula (IV) (wherein n and R4 are the same as defined above) to the reaction mixture.
The reaction may be carried out in a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane; ethers such as diethyl ether, iso- propyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; aromatic hydrocarbons such as benzene, toluene and xylene; nitriles such as acetonifrile; amides such as N, N- dimethylformamide (DMF), N, N-dimethylacetamide (DMAC) and N-methylpynolidone (NMP); urea such as l,3-dimethyl-2-imidazolidinone (DMI); sulfoxides such as dimethylsulfoxide (DMSO); and others. Optionally, two or more of the solvents selected from the listed above can be mixed and used.
The reaction temperature can be optionally set depending on the compounds to be reacted. The reaction temperature is usually, but not limited to, about 20°C to 50°C. The reaction may be conducted for, usually, 30 minutes to 10 hours and preferably 1 to 24 hours.
The reaction can be advantageously carried out in the presence of a base including, for instance, organic amines such as pyridine, friethylamine and N,N-diisopropylethylamine, dimethylaniline, diethylaniline, 4-dimethylaminopyridine, and others.
The compound (V) is commercially available or can be prepared by the use of known techniques. [Method D]
r N
Figure imgf000035_0001
The compound of the formula (I) (wherein n, Q Q2, Q3, Q and R4 are the same as defined above) can be prepared by reacting the compound of the formula (IV) (wherein n and R4 are the same as defined above) with phosgene, diphosgene, triphosgene, 1,1-carbonyldiimidazole (CDI), or 1,1'- carbonyldi(l,2,4-triazole)(CDT) and then adding the compound of the formula (B) (wherein Q_, Q2, Q3 and Q4 are the same as defined above) to the reaction mixture.
The reaction may be carried out in a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane; ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; aromatic hydrocarbons such as benzene, toluene and xylene; nitriles such as acetonitrile; amides such as N, N- dimethylformamide (DMF), N, N-dimethylacetamide (DMAC) and N-methylpynolidone (NMP); urea such as l,3-dimethyl-2-imidazolidinone (DMI); sulfoxides such as dimethylsulfoxide (DMSO); and others. Optionally, two or more of the solvents selected from the listed above can be mixed and used.
The reaction temperature can be optionally set depending on the compounds to be reacted. The reaction temperatare is usually, but not limited to, about 20°C to 50°C. The reaction may be conducted for, usually, 30 minutes to 10 hours and preferably 1 to 24 hours.
[Method E]
Figure imgf000035_0002
The compound of the formula (I) (wherein n, Qi, Q2, Q3, Q4 and R4 are the same as defined above) can be prepared by reacting the compound of the formula (TV) (wherein n and R4 are the same as defined above) with the compound of the formula (V) (wherein Lj is the same as defined above) and then adding the compound of the formula (TT) (wherein Qls Q2, Q3 and Q are the same as defined above) to the reaction mixture. Qi, Q2, Q3 and Q4 and R4.
The reaction may be carried out in a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane; ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; aromatic hydrocarbons such as benzene, toluene and xylene; nitriles such as acetonitrile; amides such as N, N- dimethylformamide (DMF), N, N-dimethylacetamide (DMAC) and N-methylpynolidone (NMP); urea such as l,3-dimethyl-2-imidazolidinone (DMT); sulfoxides such as dimethylsulfoxide (DMSO); and others. Optionally, two or more of the solvents selected from the listed above can be mixed and used.
The reaction temperature can be optionally set depending on the compounds to be reacted. The reaction temperatare is usually, but not limited to, about 20°C to 50°C. The reaction may be conducted for, usually, 30 minutes to 10 hours and preferably 1 to 24 hours.
The reaction can be advantageously canied out in the presence of a base including, for instance, organic amines such as pyridine, friethylamine and NN-diisopropylethylamine, dimethylaniline, diethylaniline, 4-dimethylaminopyridine, and others.
[Method F]
Figure imgf000036_0001
The compound of the formula (I-a), (I-b) and (I-c) (wherein n and R4 are the same as defined above) can be prepared by the following procedures.
In the Step F-l, the compound of the formula (I-a) (wherein n and R4 are the same as defined above) can be prepared in the similar manner as described in Method [A], [B], [C], [D] or [E] for the preparation of the compound of the formula (I) by using a compound of the formula (VT) instead of the compound of the formula (IT).
In the Step F-2, the compound of the formula (I-b) (wherein n and R4 are the same as defined above) can be prepared by reacting the compound of the formula (I-a) (wherein n and R4 are the same as defined above) with an acid such as hydrochloric acid.
The reaction may be carried out in a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane; ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; alcohols such as methanol, ethanol; water and others. Optionally, two or more of the solvents selected from the listed above can be mixed and used.
The reaction temperature can be optionally set depending on the compounds to be reacted. The reaction temperature is usually, but not limited to, about 20°C to 100°C. The reaction may be conducted for, usually, 30 minutes to 10 hours and preferably 1 to 24 hours.
In the Step F-3, the compound of the formula (I-c) (wherein n and R4 are the same as defined above) can be prepared by reacting the compound of the formula (I-b) (wherein n and R4 are the same as defined above) with reducing agent such as sodium borohydride or lithium aluminum hydride.
The reaction may be carried out in a solvent including, for instance, ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; alcohols such as methanol, ethanol, isopropanol, and others. Optionally, two or more of the solvents selected from the listed above can be mixed and used.
The reaction temperature can be optionally set depending on the compounds to be reacted. The reaction temperature is usually, but not limited to, about 20°C to 50°C. The reaction may be conducted for, usually, 30 minutes to 10 hours and preferably 1 to 24 hours.
The compound of the formula (VI) is commercially available or can be prepared by the use of known techniques. CHAPTER B (EMBODIMENT OF THE INVENTION)
The compound of the formula (I) of the present invention can be, but not limited to be, prepared by combining various known methods. In some embodiments, one or more of the substitaents, such as amino group, carboxyl group, and hydroxyl group of the compounds used as starting materials or intermediates are advantageously protected by a protecting group known to those skilled in the art. Examples of the protecting groups are described in "Protective Groups in Organic Synthesis (3rd Edition)" by Greene and Wuts, John Wiley and Sons, New York 1999.
The compound of the formula (I) of the present invention can be, but not limited to be, prepared by the Method [A] below.
The compound of the formula (I) of the present invention can be, but not limited to be, prepared by the Method [A], [B], [C], [D], [E], [F], [G] or [H] below.
[Method A]
Figure imgf000038_0001
The compound of the formula (I) (wherein n and R1 are the same as defined above) can be prepared by reacting the compound of the formula (II) and the compound of the formula (DI) (wherein Lj represents halogen atom such as chlorine, bromine, or iodine atom) and then adding the compound of the formula (TV) (wherein n, R1 are the same as defined above) to the reaction mixtare.
The reaction may be carried out in a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane; ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; aromatic hydrocarbons such as benzene, toluene and xylene; nitriles such as acetonitrile; amides such as N, N- dimethylformamide (DMF), N, N-dimethylacetamide (DMAC) and N-methylpynolidone (NMP); urea such as l,3-dimethyl-2-imidazolidinone (DMI); sulfoxides such as dimethylsulfoxide (DMSO); and others. Optionally, two or more of the solvents selected from the listed above can be mixed and used. The reaction temperature can be optionally set depending on the compounds to be reacted. The reaction temperatare is usually, but not limited to, about 20°C to 50°C. The reaction may be conducted for, usually, 30 minutes to 10 hours and preferably 1 to 24 hours.
The reaction can be advantageously carried out in the presence of a base including, for instance, organic amines such as pyridine, triethylamine and NN-diisopropylethylamine, dimethylaniline, diethylaniline, 4-dimethylaminopyridine, and others.
The compound (DI) and (IV) are commercially available or can be prepared by the use of known techniques.
[Method B]
Figure imgf000039_0001
The compound of the formula (I) (wherein n and R1 are the same as defined above) can be prepared by the reaction of the compound of the formula (II) and the compound of the formula (V) (wherein n and R1 are the same as defined above).
The reaction may be carried out in a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane; ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; aromatic hydrocarbons such as benzene, toluene and xylene; nitriles such as acetonitrile; amides such as N, N- dimethylformamide (DMF), N, N-dimethylacetamide (DMAC) and N-methylpynolidone (NMP); urea such as l,3-dimethyl-2-imidazolidinone (DMI); sulfoxides such as dimethylsulfoxide (DMSO); and others. Optionally, two or more of the solvents selected from the listed above can be mixed and used.
The reaction can be carried out in the presence of organic base such as pyridine or friethylamine.
The reaction temperatare can be optionally set depending on the compounds to be reacted. The reaction temperatare is usually, but not limited to, about room temperature to 100°C. The reaction may be conducted for, usually, 30 minutes to 48 hours and preferably 1 to 24 hours.
The compound (V) can be prepared by the use of known techniques or are commercially available. [Method C]
Figure imgf000040_0001
(I)
The compound of the formula (I) (wherein n and R1 are the same as defined above) can be prepared by reacting the compound of the formula (TT) with phosgene, diphosgene, triphosgene, 1,1-carbonyldiimidazole (CDI), or l, -carbonyldi(l,2,4-triazole)(CDT), and then adding the compound of the formula (IV) (wherein n and R1 are the same as defined above) to the reaction mixture.
The reaction may be ca ied out in a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane; ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; aromatic hydrocarbons such as benzene, toluene and xylene; nitriles such as acetonitrile; amides such as N, N- dimethylformamide (DMF), N, N-dimethylacetamide (DMAC) and N-methylpynolidone (NMP); urea such as l,3-dimethyl-2-imidazolidinone (DMI); sulfoxides such as dimethylsulfoxide (DMSO); and others. Optionally, two or more of the solvents selected from the listed above can be mixed and used.
The reaction temperature can be optionally set depending on the compounds to be reacted. The reaction temperatare is usually, but not limited to, about 20°C to 50°C. The reaction may be conducted for, usually, 30 minutes to 10 hours and preferably 1 to 24 hours.
Phosgene, diphosgene, triphosgene, CDI, and CDT are commercially available.
[Method D]
H2N
Figure imgf000040_0002
The compound of the formula (I) (wherein n and R1 are the same as defined above) can be prepared by reacting the compound of the formula (IV) (wherein n and R1 are the same as defined above) with phosgene, diphosgene, triphosgene, 1,1-carbonyldiimidazole (CDI), or 1,1'- carbonyldi(l,2,4-triazole)(CDT) and then adding the compound of the formula (IT) (wherein R1 is the same as defined above) to the reaction mixture.
The reaction may be carried out in a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane; ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; aromatic hydrocarbons such as benzene, toluene and xylene; nitriles such as acetonitrile; amides such as N, N- dimethylformamide (DMF), N, Ν-dimethylacetamide (DMAC) and Ν-methylpynolidone (ΝMP); urea such as l,3-dimethyl-2-imidazolidinone (DMI); sulfoxides such as dimethylsulfoxide (DMSO); and others. Optionally, two or more of the solvents selected from the listed above can be mixed and used.
The reaction temperature can be optionally set depending on the compounds to be reacted. The reaction temperature is usually, but not limited to, about 20°C to 50°C. The reaction may be conducted for, usually, 30 minutes to 10 hours and preferably 1 to 24 hours.
[Method E]
Figure imgf000041_0001
The compound of the formula (I) (wherein n and R1 are the same as defined above) can be prepared by reacting the compound of the formula (IV) (wherein n and R1 are the same as defined above) and the compound of the formula (ITT) (wherein ,χ is the same as defined above), and then adding the compound of the formula (II) to the reaction mixture.
The reaction may be carried out in a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane; ethers such as diethyl ether, iso- propyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; aromatic hydrocarbons such as benzene, toluene and xylene; nitriles such as acetonitrile; amides such as N, N- dimethylformamide (DMF), N, N-dimethylacetamide (DMAC) and N-methylpynolidone (NMP); urea such as l,3-dimethyl-2-imidazolidinone (DMI); sulfoxides such as dimethylsulfoxide (DMSO); and others. Optionally, two or more of the solvents selected from the listed above can be mixed and used.
The reaction temperature can be optionally set depending on the compounds to be reacted. The reaction temperatare is usually, but not limited to, about 20°C to 50 °C. The reaction may be conducted for, usually, 30 minutes to 10 hours and preferably 1 to 24 hours.
The reaction can be advantageously carried out in the presence of a base including, for instance, organic amines such as pyridine, friethylamine and N,N-diisopropylethylamine, dimethylaniline, diethylaniline, 4-dimethylaminopyridine, and others.
[Method F]
Figure imgf000042_0001
The compound of the formula (I) (wherein n and R1 are the same as defined above) can be prepared by the following procedures in three steps;
In the Step F-1, the compound of the formula (VET) (wherein n and R1 are the same as defined above) can be prepared by reacting the compound of the formula (VI) with the compound of the formula (V) (wherein n and R1 are the same as defined above) in a similar manner described in Method B for the preparation of the compound of the formula (I). In the Step F-2, the compound of the formula (VIII) (wherein n and R1 are the same as defined above) can be prepared by reacting the compound of the formula (VET) (wherein n and R1 are the same as defined above) with an acid such as hydrochloric acid.
The reaction may be carried out in a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane; ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; alcohols such as methanol, ethanol; water and others. Optionally, two or more of the solvents selected from the listed above can be mixed and used.
The reaction temperatare can be optionally set depending on the compounds to be reacted. The reaction temperatare is usually, but not limited to, about 20°C to 100°C. The reaction may be conducted for, usually, 30 minutes to 10 hours and preferably 1 to 24 hours.
In the Step F-3, the compound of the formula (I) (wherein n and R1 are the same as defined above) can be prepared by reacting the compound of the formula (VDI) (wherein n and R1 are the same as defined above) with reducing agent such as sodium borohydride or lithium aluminum hydride.
The reaction may be carried out in a solvent including, for instance, ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; alcohols such as methanol, ethanol, isopropanol, and others. Optionally, two or more of the solvents selected from the listed above can be mixed and used.
The reaction temperatare can be optionally set depending on the compounds to be reacted. The reaction temperatare is usually, but not limited to, about 20°C to 50°C. The reaction may be conducted for, usually, 30 minutes to 10 hours and preferably 1 to 24 hours.
The compound (VI) is commercially available or can be prepared by the use of known techniques.
[Method G]
Figure imgf000044_0001
(ll-a) (I-a) similar procedure described d [A] -[E], using (ll-a')
Figure imgf000044_0003
Figure imgf000044_0002
(ll-a1) (I-a1)
The stereoisomeric form of the compound (I), R form (I-a) (wherein n and R1 are the same as defined above) can be prepared in the similar manner as described in Method [A], [B], [C], [D], or [E] for the preparation of the compound of the formula (I) by using a compound of the formula (II- a) instead of the compound of the formula (II).
The stereoisomeric form of the compound (I), S form (I-a') (wherein n and R1 are the same as defined above) can be prepared in the similar manner as described in Method [A], [B], [C], [D], or [E] for the preparation of the compound of the formula (I) by using a compound of the formula (II- a') instead of the compound of the formula (IT).
The compound (U-a) or (ll-a') can be prepared by the use of known techniques.
CHAPTER DI (EMBODIMENT OF THE INVENTION)
The compound of the formula (T) of the present invention can be, but not limited to be, prepared by combining various known methods. In some embodiments, one or more of the substituents, such as amino group, carboxyl group, and hydroxyl group of the compounds used as starting materials or intermediates are advantageously protected by a protecting group known to those skilled in the art. Examples of the protecting groups are described in "Protective Groups in Organic Synthesis (3rd Edition)" by Greene and Wuts, John Wiley and Sons, New York 1999.
The compound of the formula (I) of the present invention can be, but not limited to be, prepared by the Method [A] below. [Method A] o
Figure imgf000045_0001
(ii) (i)
The compound of the formula (1) (wherein R1 is the same as defined above) can be prepared by the reaction of the compound of the formula (IT) with the compound of the formula (HI) (wherein R1 is the same as defined above and Li represents a leaving group including, for instance, hydroxy, halogen atom such as chlorine, bromine, or iodine atom, or azole such as imidazole or triazole.).
The reaction may be carried out in a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane; ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; aromatic hydro- carbons such as benzene, toluene and xylene; nitriles such as acetonitrile; amides such as N,N- dimethylformamide (DMF), N,N-dimethylacetamide (DMAC) and N-methylpynolidone (NMP); ureas such as l,3-dimethyl-2-imidazolidinone (DMI); sulfoxides such as dimethylsulfoxide (DMSO); and others. Optionally, two or more of the solvents selected from the listed above can be mixed and used.
The reaction temperatare can be optionally set depending on the compounds to be reacted. The reaction temperatare is usually, but not limited to, about 0°C to 50°C. The reaction may be conducted for, usually, 30 minutes to 24 hours and preferably 1 to 10 hours.
The reaction can be advantageously carried out in the presence of a base including, for instance, organic amines such as pyridine, friethylamine and N,N-diisopropylethylamine, dimethylaniline, diethylaniline, 4-dimethylaminopyridine, and others.
When Li is hydroxy, the reaction can be advantageously carried out using coupling agent including, for instance, hydroxybenzotriazole, carbodiimides such as N, N-dicyclohexylcarbodi- imide and l-(3-dimethyl-aminopropyl)-3-ethylcarbodiimide; carbonyldiazoles such as 1,1'- carbonyldi(l,3-imiazole)(CDI) and l,l'-carbonyldi(l,2,4-triazole)(CDT), and the like. The compound (B) and (IH) are commercially available or can be prepared by the use of known techniques.
[Method B]
Figure imgf000046_0001
(II) V) (I-a)
The compound of the formula (I-a) (wherein n is 1 to 6; and Xj is OR12, SR12 or N(R12)(R13) (in which R12 and R13 are the same as defined above)) can be, but not limited to be, prepared by the following procedures.
In Step B-1, the compound of the formula (V) (wherein n is 1 to 6; L represents a leaving group including, for instance, hydroxy, halogen atom such as chlorine, bromine, or iodine atom, or azole such as imidazole or triazole; and L2 represents a leaving group including, for instance, halogen atom such as chlorine, bromine, or iodine atom) can be prepared in a similar manner as described in Method [A] by using a compound of the formula (IV) (wherein n, LΪ and L2 are the same as defined above) instead of the compound of the formula (ITT).
In Step B-2, the compound of the formula (I-a) (wherein n and X_ are the same as defined above) can be, but not limited to be, prepared by the reaction of the compound of the formula (V) (wherein n and L2 are the same as defined above) with the compound of the formula (VI) (wherein Xi is the same as defined above).
The reaction may be carried out in a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane; ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; aromatic hydrocarbons such as benzene, toluene and xylene; nitriles such as acetonitrile; amides such as N,N- dimethylformamide (DMF), NN-dimethylacetamide (DMAC) and N-methylpynolidone (NMP); ureas such as l,3-dimethyl-2-imidazolidinone (DMI); sulfoxides such as dimethylsulfoxide (DMSO); and others. Optionally, two or more of the solvents selected from the listed above can be mixed and used. The reaction temperature can be optionally set depending on the compounds to be reacted. The reaction temperatare is usually, but not limited to, about 0°C to 50°C. The reaction may be conducted for, usually, 30 minutes to 24 hours and preferably 1 to 10 hours.
The reaction can be advantageously canied out in the presence of a base including, for instance, organic amines such as pyridine, triethylamine and NN-diisopropylethylamine, dimethylaniline, diethylaniline, 4-dimethylaminopyridine, and others.
The compound (IV) and (Vf) are commercially available or can be prepared by the use of known techniques.
CHAPTER TV (EMBODIMENT OF THE INVENTION)
The compound of the formula (I) of the present invention can be, but not limited to be, prepared by combining various known methods. In some embodiments, one or more of the substitaents, such as amino group, carboxyl group, and hydroxyl group of the compounds used as starting materials or intermediates are advantageously protected by a protecting group known to those skilled in the art. Examples of the protecting groups are described in "Protective Groups in Organic Synthesis (3rd Edition)" by Greene and Wuts, John Wiley and Sons, New York 1999.
The compound of the formula (I) of the present invention can be, but not limited to be, prepared by the Method [A], [B], [C], [D], or [E] below.
[Method A]
Figure imgf000047_0001
The compound of the formula (I) (wherein m, p, Qb Q2, Q3, R and X are the same as defined above) can be prepared by reacting the compound of the formula ( T) (wherein Q Q2 and Q3 are the same as defined above) and the compound of the formula (DI) (wherein Li represents a leaving group including halogen atom such as chlorine, bromine, or iodine atom) and then adding the compound of the formula (TV) (wherein m, p, R and X are the same as defined above) to the reaction mixture. The reaction may be carried out in a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane; ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; aromatic hydrocarbons such as benzene, toluene and xylene; nitriles such as acetonitrile; amides such as N, N-dimethylformamide (DMF), N, N-dimethylacetamide (DMAC) and Ν-methylpyrrolidone (ΝMP); urea such as l,3-dimethyl-2-imidazolidinone (DMI); sulfoxides such as dimethylsulfoxide (DMSO); and others. Optionally, two or more of the solvents selected from the listed above can be mixed and used.
The reaction temperatare can be optionally set depending on the compounds to be reacted. The reaction temperatare is usually, but not limited to, about 20°C to 50 °C. The reaction may be conducted for, usually, 30 minutes to 24 hours and preferably 1 to 10 hours.
The reaction can be advantageously canied out in the presence of a base including, for instance, organic amines such as pyridine, triethylamine and NΝ-diisopropylethylamine, dimethylaniline, diethylaniline, 4-dimethylaminopyridine, and others.
The compound of the formula (DI) and (IV) are commercially available or can be prepared by the use of known techniques.
[Meth od B]
Figure imgf000048_0001
The compound of the formula (I) (wherein m, p, Qi, Q2, Q3, R and X are the same as defined above) can be prepared by the reaction of the compound of the formula (II) (wherein Q_, Q2 and Q3 are the same as defined above) and the compound of the formula (V) (wherein m, p, R and X are the same as defined above).
The reaction may be carried out in a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane; ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; aromatic hydrocarbons such as benzene, toluene and xylene; nitriles such as acetonitrile; amides such as N, N- dimethylformamide (DMF), N, N-dimethylacetamide (DMAC) and Ν-methylpynolidone (ΝMP); urea such as l,3-dimethyl-2-imidazolidinone (DMT); sulfoxides such as dimethylsulfoxide (DMSO); and others. Optionally, two or more of the solvents selected from the listed above can be mixed and used.
The reaction can be carried out in the presence of organic base such as pyridine or triethylamine.
The reaction temperatare can be optionally set depending on the compounds to be reacted. The reaction temperatare is usually, but not limited to, about room temperature to 100°C. The reaction may be conducted for, usually, 30 minutes to 24 hours and preferably 1 to 10 hours.
The compound (V) can be prepared by the use of known techniques or are commercially available.
[Method C]
Figure imgf000049_0001
The compound of the formula (I) (wherein m, p, Qj, Q2, Q3, R and X are the same as defined above) can be prepared by reacting the compound of the formula (IT) (wherein Qi, Q2 and Q3 are the same as defined above) with phosgene, diphosgene, triphosgene, 1,1-carbonyldiimidazole (CDI), or l,r-carbonyldi(l,2,4-triazole)(CDT), and then adding the compound of the formula (IV) (wherein m, p, R and X are the same as defined above) to the reaction mixtare.
The reaction may be canied out in a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane; ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; aromatic hydrocarbons such as benzene, toluene and xylene; nitriles such as acetonitrile; amides such as N, N- dimethylformamide (DMF), N, N-dimethylacetamide (DMAC) and N-methylpynolidone (NMP); urea such as l,3-dimethyl-2-imidazolidinone (DMI); sulfoxides such as dimethylsulfoxide (DMSO); and others. Optionally, two or more of the solvents selected from the listed above can be mixed and used.
The reaction temperatare can be optionally set depending on the compounds to be reacted. The reaction temperature is usually, but not limited to, about 20°C to 50°C. The reaction may be conducted for, usually, 30 minutes to 24 hours and preferably 1 to 10 hours.
Phosgene, diphosgene, triphosgene, CDI, and CDT are commercially available. [Method D]
Figure imgf000050_0001
The compound of the formula (I) (wherein m, p, Qi, Q2, Q3, R and X are the same as defined above) can be prepared by reacting the compound of the formula (IV) (wherein m, p, R and X are the same as defined above) with phosgene, diphosgene, triphosgene, 1,1-carbonyldiimidazole (CDI), or l, -carbonyldi(l,2,4-triazole)(CDT) and then adding the compound of the formula (TT) (wherein Qj, Q2 and Q3 are the same as defined above) to the reaction mixtare.
The reaction may be carried out in a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane; ethers such as diethyl ether, isopropyl ether, dioxane and tefrahydrofuran (THF) and 1,2-dimethoxyethane; aromatic hydrocarbons such as benzene, toluene and xylene; nitriles such as acetonitrile; amides such as N, N- dimethylformamide (DMF), N, N-dimethylacetamide (DMAC) and N-methylpynolidone (NMP); urea such as l,3-dimethyl-2-imidazolidinone (DMI); sulfoxides such as dimethylsulfoxide (DMSO); and others. Optionally, two or more of the solvents selected from the listed above can be mixed and used.
The reaction temperatare can be optionally set depending on the compounds to be reacted. The reaction temperatare is usually, but not limited to, about 20°C to 50°C. The reaction may be conducted for, usually, 30 minutes to 24 hours and preferably 1 to 10 hours.
[Method E]
Figure imgf000050_0002
The compound of the formula (I) (wherein m, p, Qi, Q2, Q3, R and X are the same as defined above) can be prepared by reacting the compound of the formula (TV) (wherein m, p, R and X are the same as defined above) and the compound of the formula (DT) (wherein Li is the same as defined above), and then adding the compound of the formula (D) (wherein Q Q2 and Q3 are the same as defined above) to the reaction mixture.
The reaction may be carried out in a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane; ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; aromatic hydrocarbons such as benzene, toluene and xylene; nitriles such as acetonitrile; amides such as N, N- dimethylformamide (DMF), N, N-dimethylacetamide (DMAC) and N-methylpynolidone (NMP); urea such as l,3-dimethyl-2-imidazolidinone (DMI); sulfoxides such as dimethylsulfoxide (DMSO); and others. Optionally, two or more of the solvents selected from the listed above can be mixed and used.
The reaction temperatare can be optionally set depending on the compounds to be reacted. The reaction temperature is usually, but not limited to, about 20°C to 50 °C. The reaction may be conducted for, usually, 30 minutes to 24 hours and preferably 1 to 10 hours.
The reaction can be advantageously carried out in the presence of a base including, for instance, organic amines such as pyridine, triethylamine and NN-diisopropylethylamine, dimethylaniline, diethylaniline, 4-dimethylaminopyridine, and others.
Preparation of compound of the formula (DT)
The compound of the formula (II) (wherein Qi, Q2 and Q3 are the same as defined above) can be prepared by the following procedures.
Figure imgf000051_0001
(VII) (VI)
Figure imgf000051_0002
(VIII) (II) In the Step i-l, the compound of the formula (VH) (wherein Q_, Q2 and Q3 are the same as defined above and P. represents alkyl such as methyl or ethyl) can be prepared by the reduction of the compound of the formula (VI) (wherein Pi, Qi, Q2 and Q3 are the same as defined above and P represents amino or nitro).
The reduction can be carrid out by using the agent including, for instance, metal such as lithium, sodium, and the like.
The reaction can be carried out in a solvent including, for instance, liquid ammonia; alkylamine such as methylamine, ethylamine, and ethylenediamine (EDA); and alcohols such as methanol, ethanol, isopropanol, tert-butanol and others. Optionally, two or more of the solvents selected from the listed above can be mixed and used.
Solvent including, for instance, ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane can be used as a co-solvent.
The reaction temperature can be optionally set depending on the compounds to be reacted. The reaction temperature is usually, but not limited to, about -78°C to 50 °C. The reaction may be conducted for, usually, 30 minutes to 24 hours and preferably 1 to 10 hours.
In the Step i-2, the compound of the formula (VIII) (wherein Qi, Q2 and Q3 are the same as defined above) can be prepared by the reaction of the compound of the formula (VH) (wherein Pi, Qi, Q2 and Q3 are the same as defined above are the same as defined above) with an acid such as hydrochloric acid.
The reaction may be canied out in a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane; ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; alcohols such as methanol, ethanol; water and others. Optionally, two or more of the solvents selected from the listed above can be mixed and used.
The reaction temperature can be optionally set depending on the compounds to be reacted. The reaction temperature is usually, but not limited to, about 20°C to 100°C. The reaction may be conducted for, usually, 30 minutes to 24 hours and preferably 1 to 10 hours.
In the Step i-3, the compound of the formula (II) (wherein Qi, Q2 and Q3 are the same as defined above) can be prepared by reacting the compound of the formula (VDT) (wherein Q1} Q2 and Q3 are the same as defined above) with a reducing agent such as sodium borohydride or lithium aluminum hydride. The reaction may be carried out in a solvent including, for instance, ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; alcohols such as methanol, ethanol, isopropanol, and others. Optionally, two or more of the solvents selected from the listed above can be mixed and used.
The reaction temperature can be optionally set depending on the compounds to be reacted. The reaction temperatare is usually, but not limited to, about 20°C to 50°C. The reaction may be conducted for, usually, 30 minutes to 24 hours and preferably 1 to 10 hours.
The compound (VI) is commercially available or can be prepared by the use of known techniques.
Alternative preparation method of compound of the formula (Vπi)
The compound of the formula (VDT) can also be prepared by the following procedures.
Figure imgf000053_0001
(IX) (X) (VIII)
Figure imgf000053_0002
(XI) (XII)
In the Step ii-1, the compound of the formula (X) (wherein Q Q2 and Q3 are the same as defined above) can be prepared by the nitration of the compound of the formula (IX) (wherein Qi, Q2 and Q3 are the same as defined above.) using the agent including, for instance, nitroric acid, potassium nitrate, a combination agent of dinifrogen pentoxide and sulphur dioxide, a combination agent of dinifrogen pentoxide, nitromethane and sodium bisulfonate, a combination agent of dimethylsulfoxide, acetic anhydride.
The reaction can be carried out without solvent or in a solvent including, for instance, acid such as acetic acid, sulfonic acid, trifluoroacetic acid. Optionally, two or more of the solvents selected from the listed above can be mixed and used. The reaction temperature can be optionally set depending on the compounds to be reacted. The reaction temperature is usually, but not limited to, about -15°C to 100°C. The reaction may be conducted for, usually, 30 minutes to 24 hours and preferably 1 to 10 hours.
The compound of the formula (X) (wherein Qi, Q2 and Q3 are the same as defined above) can alternatively be prepared by the following procedures.
In the Step ii-a, the compound of the formula (XI) (wherein Q'ι, Q'2 and Q'3 independently represent N, N'-O" or CH, with the proviso that at least one of Q Q2 and Q3 is N-O") can be prepared by the oxydation of the compound of the formula (DC) (wherein Q_, Q2 and Q3 are the same as defined above) using an agent including, for instance, hydrogen peroxide, m-chloro- perbenzoic acid, dimethyldioxirane and the like.
The reaction can be carried out in a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane; acid such as acetic acid, and water. Optionally, two or more of the solvents selected from the listed above can be mixed and used.
The reaction temperature can be optionally set depending on the compounds to be reacted. The reaction temperature is usually, but not limited to, about -15°C to 100°C. The reaction may be conducted for, usually, 30 minutes to 24 hours and preferably 1 to 10 hours.
In the Step ii-b, the compound of the formula (XII) (wherein Q'ι, Q'2 and Q'3 are the same as defined above) can be prepared by the nitration of the compound of the formula (XT) (wherein Q'j, Q'2 and Q'3 are the same as defined above) in a similar manner as described for the preparation of the compound of the formula (X).
In the Step ii-c, the compound of the formula (X) (wherein Qi, Q2 and Q3 are the same as defined above) can be prepared by the reduction of the compound of the formula (XH) (wherein Q'j, Q'2 and Q'3 are the same as defined above) using the agent including, for instance, triphenyl phosphine, xriethyl phosphite, trimethyl phosphite, methanesulfonyl chloride, a combination agent of lithium chloride and sodium borohydride, and the like.
The reaction can be carried out in a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane; ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; aromatic hydrocarbons such as benzene, toluene and xylene, and the like. Optionally, two or more of the solvents selected from the listed above can be mixed and used. The reaction temperature can be optionally set depending on the compounds to be reacted. The reaction temperature is usually, but not limited to, about 0°C to 100°C. The reaction may be conducted for, usually, 30 minutes to 24 hours and preferably 1 to 10 hours.
In the Step ii-2, the compound of the formula (VIH) (wherein Qi, Q2 and Q3 are the same as defined above) can be prepared by reducing nitro group of the compound of the formula (X) (wherein Q Q2 and Q3 are the same as defined above.) using an agent including, for instance, metals such as zinc and iron in the presence of acid including, for instance, hydrochloric acid and acetic acid and stannous chloride, or by hydrogenation using a catalyst including, for instance, palladium on carbon and platinum on carbon.
The reaction can be carried out in a solvent including, for instance, ethers such as diethyl ether, isopropyl ether, dioxane, tetrahydrofuran (THF) and 1,2-dimethoxyethane, aromatic hydrocarbons such as benzene, toluene and xylene, alcohols such as methanol, ethanol, 1-propanol, isopropanol and tert-butanol, water and others.
The reaction temperature can be optionally set depending on the compounds to be reacted. The reaction temperatare is usually, but not limited to, about 20°C to 120°C. The reaction may be conducted for, usually, 30 minutes to 24 hours and preferably 1 to 10 hours.
Alternatively, the compound of the formula (VHT) (wherein Qi, Q2 and Q3 are the same as defined above) can be prepared by reduction of the compound of the formula (XIT) (wherein Q'_, Q'2 and Q'3 are the same as defined above) as shown in the Step ii-3.
The reduction can be carried out using an agent including, for instance, metals such as titanium and iron, and sodium hypophosphite together with a catalyst including, for instance, palladium on carbon and platinum on carbon.
The reaction can be canied out in a solvent including, for instance, ethers such as diethyl ether, isopropyl ether, dioxane, tetrahydrofuran (THF) and 1,2-dimethoxyethane, aromatic hydrocarbons such as benzene, toluene and xylene, alcohols such as methanol, ethanol, 1-propanol, isopropanol and tert-butanol, acid such as acetic acid, water and others.
The reaction temperature can be optionally set depending on the compounds to be reacted. The reaction temperature is usually, but not limited to, about 20°C to 120°C. The reaction may be conducted for, usually, 30 minutes to 24 hours and preferably 1 to 10 hours.
The compound (IX) is commercially available or can be prepared by the use of known techniques.
The compound of the formula (VIH) can also be prepared by the following procedures.
Figure imgf000056_0001
(XIV) Step iii-4 (XVI)
In the Step iii-1, the compound of the formula (VIH) (wherein Qi, Q2 and Q3 are the same as defined above) can be prepared by the reaction of the compound of the formula (XHI) (wherein Q Q2 and Q3 are the same as defined above.) using the agent including, for instance, p- toluenesulfonyl isocyanate.
The reaction can be carried in a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane; ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; aromatic hydrocarbons such as benzene, toluene and xylene and others. Optionally, two or more of the solvents selected from the listed above can be mixed and used.
The reaction temperature can be optionally set depending on the compounds to be reacted. The reaction temperatare is usually, but not limited to, about 20 °C to 100 °C. The reaction may be conducted for, usually, 30 minutes to 24 hours and preferably 1 to 10 hours.
In the Step iii-2, the compound of the formula (XIV) (wherein Q_, Q2 and Q3 are the same as defined above and L2 represents a leaving group including halogen atom such as chlorine, bromine, or iodine atom; and alkylsulfonyloxy such as frifluoromethylsulfonyloxy) can be prepared by the reaction of the compound of the formula (XHI) (wherein Q1} Q2 and Q3 are the same as defined above.) using the agent including, for instance, halogenating reagent such as POCl3, POBr3, PC15 and the like; or sulfonyl chloride such as xrifluoromethylsulfonyl chloride.
The reaction may be carried out in a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and l,2-dichloroethane;such as ethers such as dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; aromatic hydrocarbons such as benzene, toluene, and xylene, and others. Optionally, two or more of the solvents selected from the listed above can be mixed and used.
The reaction can be advantageously conducted in the presence of a base, including, for instance, such as pyridine, triethylamine and NN-diisopropylethylamine, dimethylaniline, diethylaniline, and others.
The reaction temperatare is usually, but not limited to, about 40°C to 200°C and preferably about 20°C to 180°C. The reaction may be conducted for, usually, 30 minutes to 24 hours and preferably 2 hours to 10 hours.
In the Step iii-3, the compound of the formula (XIV) (wherein L2, Q_, Q2 and Q3 are the same as defined above) can be prepared by the reaction of the compound of the formula (Xlfl) (wherein Qi, Q2 and Q3 are the same as defined above) using the agent including, for instance, ammonia.
The reaction can be advantageously conducted in the presence of a catalyst including, for instance, copper(T) oxide, copper(D) sulfate and the like.
The reaction may be canied out in a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane; such as ethers such as dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; aromatic hydrocarbons such as benzene, toluene, and xylene, and others. Optionally, two or more of the solvents selected from the listed above can be mixed and used.
The reaction temperature is usually, but not limited to, about 40°C to 200°C and preferably about 20°C to 180°C. The reaction may be conducted for, usually, 30 minutes to 24 hours and preferably 2 hours to 12 hours.
The compound of the formula (VIH) can also be prepared by the following procedures.
In the Step iii-4, the compound of the formula (XVI) (wherein Qi, Q2 and Q3 are the same as defined above; and P3 represents aralkyl such as benzyl, 4-methoxybenzyl, 3,4-dimethoxybenzyl) can be prepared by the reaction of the compound of the formula (XIV) (wherein L2, Qi, Q2 and Q3 are the same as defined above) with the compound of the formula (XV) (wherein P3 is the same as defined above).
The reaction can be carried out in the presence of a palladium catalyst such as tetrakis- (triphenylphosphine)palladium or a combination of a phosphine ligand and a palladium catalyst such as tri-o-tolylphosphine and palladium (H) acetate. The reaction can be advantageously carried out in the presence of a base including, for instance, cesium carbonate, sodium carbonate, potassium carbonate, barium hydroxide sodium methoxide, sodium ethoxide, potassium tert-butoxide and the like.
This reaction can be carried out in a solvent including, for instance, alcohol such as methanol, ethanol, 1-propanol, isopropanol and tert-butanol; ethers, such as dioxane, isopropyl ether, diethyl ether, 1,2-dimethoxyethane and tetrahydrofuran (THF); aromatic hydrocarbons such as benzene, toluene and xylene; nitriles such as acetonitrile; amides such as dimethylformamide (DMF) N, Ν- dimethylacetamide and Ν-methylpynolidone; sulfoxides such as dimethylsulfoxide (DMSO); water and others. Optionally, two or more of the solvents selected from the listed above can be mixed and used.
The reaction temperature can be optionally set depending on the compounds to be reacted. The reaction temperature is usually, but not limited to, about 10°C to 200°C and preferably about 50°C to 150°C. The reaction may be conducted for, usually, 30 minutes to 24 hours and preferably 1 to 12 hours.
In the Step iii-5, the compound of the formula (VIH) (wherein Qi, Q2 and Q3 are the same as defined above) can be prepared by the removal of P3 of the compound of the formula (XVI) (wherein P3, Qi, Q2 and Q3 are the same as defined above).
The removal of P3 can be done by hydrogenation using a catalyst including, for instance, palladium on carbon and palladium hydroxide. Also, the removal can be done by using a reagent including, for instance, trifluoroacetic acid, eerie ammonium nitrate (CAN) or 2,3-dichloro-5,6-dicyano-l,4- benzoquinone (DDQ), when P3 is 4-methoxybenzyl or 3,4-dimethoxybenzyl.
This reaction can be carried out in a solvent including, for instance, alcohol such as methanol, ethanol, 1-propanol, isopropanol and tert-butanol; ethers, such as dioxane, isopropyl ether, diethyl ether, 1,2-dimethoxyethane and tetrahydrofuran (THF); aromatic hydrocarbons such as benzene, toluene and xylene; ester such as ethyl acetate; water and others. Optionally, two or more of the solvents selected from the listed above can be mixed and used.
The compound (XTH) and (XV) are commercially available or can be prepared by the use of known techniques. [Method F]
Figure imgf000059_0001
(XVIII)
The compound of the formula (I) (wherein m, p, Qi, Q2, Q3, R and X are the same as defined above) can alternatively be prepared by the following procedures in three steps;
In the Step F-1, the compound of the formula (XVH) (wherein m, p, Pi, Ql5 Q2, Q3, R and X are the same as defined above) can be prepared in a similar manner as described in Method [A], [B], [C], [D] or [E] for the preparation of the compound of the formula (I) by using a compound of the formula (VH) (wherein Pi, Q Q2 and Q3 are the same as defined above) instead of the compound of the formula (H).
In the Step F-2, the compound of the formula (XVHI) (m, p, Qi, Q2, Q3, R and X are the same as defined above) can be prepared by reacting the compound of the formula (XVH) (m, p, Pb Qi, Q2, Q3, R and X are the same as defined above) with an acid such as hydrochloric acid.
The reaction may be canied out in a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane; ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; alcohols such as methanol, ethanol; water and others. Optionally, two or more of the solvents selected from the listed above can be mixed and used.
The reaction temperatare can be optionally set depending on the compounds to be reacted. The reaction temperatare is usually, but not limited to, about 20°C to 100°C. The reaction may be conducted for, usually, 30 minutes to 24 hours and preferably 1 to 10 hours. In the Step F-3, the compound of the formula (I) (wherein m, p, Q_, Q2, Q3, R and X are the same as defined above) can be prepared by reacting the compound of the formula (XVIH) (wherein m, p, Qi. Q2. Q3. and X are the same as defined above) with reducing agent such as sodium borohydride or lithium aluminum hydride.
The reaction may be carried out in a solvent including, for instance, ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; alcohols such as methanol, ethanol, isopropanol, and others. Optionally, two or more of the solvents selected from the listed above can be mixed and used.
The reaction temperatare can be optionally set depending on the compounds to be reacted. The reaction temperature is usually, but not limited to, about 20°C to 50°C. The reaction may be conducted for, usually, 30 minutes to 24 hours and preferably 1 to 10 hours.
Alternative preparation method of compound of the formula (XVHT)
Figure imgf000060_0001
The compound of the formula (XVHI) (m, p, Qi, Q2, Q3, R and X are the same as defined above) can alternatively be prepared in a similar manner as described in Method [A], [B], [C], [D] or [E] for the preparation of the compound of the formula (I) by using a compound of the formula (VHT) (wherein Qi, Q2 and Q3 are the same as defined above) instead of the compound of the formula (H).
[Method G]
Figure imgf000061_0001
Figure imgf000061_0002
The compound of the formula (I-a) (wherein m, p, Qi, Q2, Q3 and R are the same as defined above and X' is -O-, or N(RJ)-) can be prepared by the following procedures.
In the Step G-1, the compound of the formula (XXI) (wherein m, Qi, Q2 and Q3 are the same as defined above and L3 represents leaving group including, for instance, halogen atom such as chlorine, bromine, or iodine atom) can be prepared in a similar manner as described in Method [A], [B], [C], [D] or [E] for the preparation of the compound of the formula (I) by using a compound of the formula (XIX) (wherein m and L3 are the same as defined above) instead of the compound of the formula (IV), or using a compound of the formula (XX) (wherein m and L3 are the same as defined above) instead of the compound of the formula (V).
In the Step G-2, the compound of the formula (I-a) (wherein m, p, Qi, Q2, Q3, R and X' are the same as defined above) can be prepared by reacting the compound of the formula (XXI) (wherein m, L3, Qi, Q2 and Q3 are the same as defined above) and the compound of the formula (XXII) (wherein p, R and X' are the same as defined above).
The reaction may be carried out in a solvent including, for instance, halogenated hydrocarbons such as dichloromethane, chloroform and 1,2-dichloroethane; ethers such as diethyl ether, isopropyl ether, dioxane and tetrahydrofuran (THF) and 1,2-dimethoxyethane; aromatic hydrocarbons such as benzene, toluene and xylene; nitriles such as acetonitrile; amides such as N,N- dimethylformamide (DMF), NN-dimethylacetamide (DMAC) and N-methylpynolidone (NMP); ureas such as l,3-dimethyl-2-imidazolidinone (DMI); sulfoxides such as dimethylsulfoxide (DMSO); and others. Optionally, two or more of the solvents selected from the listed above can be mixed and used.
The reaction temperature can be optionally set depending on the compounds to be reacted. The reaction temperatare is usually, but not limited to, about 0°C to 50°C. The reaction may be conducted for, usually, 30 minutes to 24 hours and preferably 1 to 10 hours.
The reaction can be advantageously carried out in the presence of a base including, for instance, organic amines such as pyridine, triethylamine and NN-diisopropylethylamine, dimethylaniline, diethylaniline, 4-dimethylaminopyridine, and others.
The compound (XIX), (XX) and (XXIT) are commercially available or can be prepared by the use of known techniques.
[Method H]
Figure imgf000062_0001
in instead
Figure imgf000062_0002
Figure imgf000062_0003
(ll-a1) (I-a1)
The stereoisomeric form of the compound (I), R form (I-a) (wherein m, p, Qi, Q2, Q3, R and X are the same as defined above) can be prepared in a similar manner as described in Method [A], [B], [C], [D], or [E] for the preparation of the compound of the formula (I) by using a compound of the formula (H-a) (wherein Ql9 Q2 and Q3 are the same as defined above) instead of the compound of the formula (H).
The stereoisomeric form of the compound (I), S form (I-a') (wherein m, p, Qi, Q2, Q3, R and X are the same as defined above) can be prepared in the similar manner as described in Method [A], [B], [C], [D], or [E] for the preparation of the compound of the formula (I) by using a compound of the formula (H-a') (wherein Qi, Q2 and Q3 are the same as defined above) instead of the compound of the formula (IT).
The compound (H-a) or (H-a') can be prepared by the use of known techniques.
SALTS AND FORMULATIONS
When the compound shown by the formula (A) or a salt thereof has an asymmetric carbon in the structure, their optically active compounds and racemic mixtures are also included in the scope of the present invention.
Typical salts of the compound shown by the formula (A) include salts prepared by reaction of the compounds of the present invention with a mineral or organic acid, or an organic or inorganic base. Such salts are known as acid addition and base addition salts, respectively.
Acids to form acid addition salts include inorganic acids such as, without limitation, sulfuric acid, phosphoric acid, hydrochloric acid, hydrobromic acid, hydriodic acid and the like, and organic acids, such as, without limitation, p-toluenesulfonic acid, methanesulfonic acid, oxalic acid, p- bromophenylsulfonic acid, carbonic acid, succinic acid, citric acid, benzoic acid, acetic acid, and the like.
Base addition salts include those derived from inorganic bases, such as, without limitation, ammonium hydroxide, alkaline metal hydroxide, alkaline earth metal hydroxides, carbonates, bicarbonates, and the like, and organic bases, such as, without limitation, ethanolamine, triethylamine, tris(hydroxymethyl)aminomethane, and the like. Examples of inorganic bases include sodium hydroxide, potassium hydroxide, potassium carbonate, sodium carbonate, sodium bicarbonate, potassium bicarbonate, calcium hydroxide, calcium carbonate, and the like.
The compound of the present invention or a salt thereof, depending on its substituents, may be modified to form lower alkylesters or known other esters; and/or hydrates or other solvates. Those esters, hydrates, and solvates are included in the scope of the present invention.
The compound of the present invention may be administered in oral forms, such as, without limitation normal and enteric coated tablets, capsules, pills, powders, granules, elixirs, tinctures, solution, suspensions, syrups, solid and liquid aerosols and emulsions. They may also be administered in parenteral forms, such as, without limitation, intravenous, intraperitoneal, subcutaneous, intramuscular, and the like forms, well-known to those of ordinary skill in the pharmaceutical arts. The compounds of the present invention can be administered in infranasal form via topical use of suitable infranasal vehicles, or via transdermal routes, using transdermal delivery systems well-known to those of ordinary skilled in the art. The dosage regimen with the use of the compounds of the present invention is selected by one of ordinary skill in the arts, in view of a variety of factors, including, without limitation, age, weight, sex, and medical condition of the recipient, the severity of the condition to be treated, the route of administration, the level of metabolic and excretory function of the recipient, the dosage form employed, the particular compound and salt thereof employed.
The compounds of the present invention are preferably formulated prior to administration together with one or more pharmaceutically-acceptable excipients. Excipients are inert substances such as, without limitation carriers, diluents, flavoring agents, sweeteners, lubricants, solubilizers, suspending agents, binders, tablet disintegrating agents and encapsulating material.
Yet another embodiment of the present invention is pharmaceutical formulation comprising a compound of the invention and one or more pharmaceutically-acceptable excipients that are compatible with the other ingredients of the formulation and not deleterious to the recipient thereof. Pharmaceutical formulations of the invention are prepared by combining a therapeutically effective amount of the compounds of the invention together with one or more pharmaceutically- acceptable excipients therefore. In making the compositions of the present invention, the active ingredient may be mixed with a diluent, or enclosed within a carrier, which may be in the form of a capsule, sachet, paper, or other container. The carrier may serve as a diluent, which may be solid, semi-solid, or liquid material which acts as a vehicle, or can be in the form of tablets, pills powders, lozenges, elixirs, suspensions, emulsions, solutions, syrups, aerosols, ointments, containing, for example, up to 10% by weight of the active compound, soft and hard gelatin capsules, suppositories, sterile injectable solutions and sterile packaged powders.
For oral administration, the active ingredient may be combined with an oral, and non-toxic, pharmaceutically-acceptable carrier, such as, without limitation, lactose, starch, sucrose, glucose, sodium carbonate, mannitol, sorbitol, calcium carbonate, calcium phosphate, calcium sulfate, methyl cellulose, and the like; together with, optionally, disintegrating agents, such as, without limitation, maize, starch, methyl cellulose, agar bentonite, xanthan gum, alginic acid, and the like; and optionally, binding agents, for example, without limitation, gelatin, natural sugars, beta- lactose, com sweeteners, natural and synthetic gums, acacia, tragacanth, sodium alginate, carboxymethylcellulose, polyethylene glycol, waxes, and the like; and, optionally, lubricating agents, for example, without limitation, magnesium stearate, sodium stearate, stearic acid, sodium oleate, sodium benzoate, sodium acetate, sodium chloride, talc, and the like.
In powder forms, the carrier may be a finely divided solid which is in admixture with the finely divided active ingredient. The active ingredient may be mixed with a carrier having binding properties in suitable proportions and compacted in the shape and size desired to produce tablets. The powders and tablets preferably contain from about 1 to about 99 weight percent of the active ingredient which is the novel composition of the present invention. Suitable solid carriers are magnesium carboxymethyl cellulose, low melting waxes, and cocoa butter.
Sterile liquid formulations include suspensions, emulsions, syrups and elixirs. The active ingredient can be dissolved or suspended in a pharmaceutically acceptable earners, such as sterile water, sterile organic solvent, or a mixture of both sterile water and sterile organic solvent.
The active ingredient can also be dissolved in a suitable organic solvent, for example, aqueous propylene glycol. Other compositions can be made by dispersing the finely divided active ingredient in aqueous starch or sodium carboxymethyl cellulose solution or in a suitable oil.
The formulation may be in unit dosage form, which is a physically discrete unit containing a unit dose, suitable for administration in human or other mammals. A unit dosage form can be a capsule or tablets, or a number of capsules or tablets. A "unit dose" is a predetermined quantity of the active compound of the present invention, calculated to produce the desired therapeutic effect, in association with one or more excipients. The quantity of active ingredient in a unit dose may be varied or adjusted from about 0.1 to about 1000 milligrams or more according to the particular treatment involved.
Typical oral dosages of the present invention, when used for the indicated effects, will range from about O.Olmg /kg/day to about 100 mg/kg/day, preferably from 0.1 mg/kg/day to 30 mg/kg/day, and most preferably from about 0.5 mg/kg/day to about 10 mg/kg/day. In the case of parenteral administration, it has generally proven advantageous to administer quantities of about 0.001 to lOOmg /kg/day, preferably from 0.01 mg/kg/day to 1 mg/kg/day. The compounds of the present invention may be administered in a single daily dose, or the total daily dose may be administered in divided doses, two, three, or more times per day. Where delivery is via transdermal forms, of course, administration is continuous.
EXAMPLES
The present invention will be described as a form of examples, but they should by no means be construed as defining the metes and bounds of the present invention.
In the examples below, all quantitative data, if not stated otherwise, relate to percentages by weight.
Liquid Chromatography - Mass specfroscopy (LC-MS)
Micromass Platform LC with Shimadzu Phenomenex ODS column(4.6 mm X 30 mm) flushing a mixture of acetonitrile-water (9:1 to 1:9) at 1 ml/min of the flow rate. Mass spectra were obtained using elecfrospray (ES) ionization techniques.
High Pressure Liquid Chromatography (HPLC) : Method A
Instrument: Hewlett Packard series; Column Temperatare: 40°C; Mobile Phase: Water and Acetonitrile (each of them contains 10 mM ammonium acetate); Column: Phenomenex Luna 3u C18(2) (4.6 mm X 30 mm); Flow Rate: 1.0 mL/min; Gradient : Time (minutes) : (Water / Acetonitrile) 0 min : 9 / 1, O.lmin : 9 / 1, 1.5min : 1 / 9, 3.5min :1 / 9, 4.5 min: 9 / 1.
High Pressure Liquid Chromatography (HPLC) : Method B
Instrument: HP 1100 with DAD-detection; column: Kromasil RP-18, 60 mm x 2 mm, 3.5 μm; eluent A: 5 ml HClOyi water, eluent B: acetonitrile; gradient: 0 min 2%B, 0.5 min 2%B, 4.5 min 90%B, 6.5 min 90%B; flow rate: 0.75 ml/min; oven temp.: 30°C; UV-detection: 210 nm.
Liquid Chromatography - Mass specfroscopy (LC-MS : Method C
Instrument: Micromass Platform ZQ with HPLC Waters Alliance 2795; Column: Phenomenex Synergi 2μ Hydro-RP Mercury 20 mm x 4 mm; eluent A: 1 1 water + 0.5 ml 50% aqueous formic acid, eluent B: 1 1 acetonitrile + 0.5 ml aqueous formic acid; gradient: 0.0 min 90%A -> 2.5 min 30%A - 3.0 min 5%A - 4.5 min 5%A; flow rate: 0.0 min 1 ml/min, 2.5 min/3.0 min/4.5 min 2 ml/min; oven temp.: 50°C; UV-detection: 210 nm.
High Pressure Liquid Chromatography (HPLC) : Method D
Instrument: HP 1100 with DAD-Detection; column: Kromasil 100 RP-18, 60 mm x 2.1 mm, 3.5μm; eluent A: 5ml HC104 / 1 water, eluent B: acetonitrile; Gradient: 0 min 2%B; 0.5 min 2%B; 4.5 min 90%B; 9 min 90%B; 9.2 min 2%B; 10 min 2%B; flow rate: 0.75 ml/min; oven temp.: 30°C; UV-detection: 210 nm. Liquid Chromatography - Mass specfroscopy (LC-MS): Method E
Instrument: Micromass Platform LCZ with HPLC Agilent Series 1100; Column: Phenomenex Synergi 2μ Hydro-RP Mercury 20 mm x 4 mm; eluent A: 1 1 water + 0.5 ml 50% aqueous formic acid, eluent B: 1 1 acetonitrile + 0.5 ml aqueous formic acid; gradient: 0.0 min 90%A - 2.5 min 30%A -> 3.0 min 5%A ■» 4.5 min 5%A; flow rate: 0.0 min 1 ml/min, 2.5 min/3.0 min/4.5 min 2 ml/min; oven temp.: 50°C; UV-detection: 210 nm.
Liquid Chromatography - Mass specfroscopy (LC-MS): Method F
Instrument MS: Micromass ZQ; instrument HPLC: Waters Alliance 2795; Column: Merck Chromolith SpeedROD RP-18e 50 mm x 4.6 mm; eluent A: water + 500 μl 50% aqueous formic acid / 1; eluent B: acetonifrile + 500 μl 50% aqueous formic acid / 1; gradient: 0.0 min 10%B-^ 3.0 min 95%B-> 4.0 min 95%B; oven temp.: 35°C; flow rate: 0.0 min 1.0 m_/min- 3.0 min 3.0 ml/min- 4.0 min 3.0 ml/min; UV-detection: 210 nm.
Liquid Chromatography - Mass specfroscopy (LC-MS') : Method G
Instrument MS: Micromass ZQ; instrument HPLC: HP 1100 Series; UV DAD; column: Grom-Sil 120 ODS-4 HE 50 mm x 2 mm, 3.0 μm; eluent A: water + 500 μl 50% aqueous formic acid / 1, eluent B: acetonifrile + 500 μl 50% aqueous formic acid / 1; gradient: 0.0 min 0%>B - 2.9 min 70%B -> 3.1 min 90%B -» 4.5 min 90%B; oven temp.: 50 °C; flow rate: 0.8 ml min; UV- detection: 210 nm.
Preparative HPLC purifications are performed on a GROM-SIL 120 ODS-4 HE 10 μm, 250 mm x 30 mm column with acetonitrile/water gradients.
Mass determination
The mass determinations were carried out by MAT95 (Finnigan MAT).
Melting points are unconected.
!H NMR spectra were recorded using either Bruker DRX-300 (300 MHz for H) spectrometer or Bracker 500 UlfraShieled™ (500 MHz for IH). Chemical shifts are reported in parts per million (ppm) with tetramethylsilane (TMS) as an internal standard at zero ppm. Coupling constant (J) are given in hertz and the abbreviations s, d, t, q, m, and br refer to singlet, doblet, triplet, quartet, multiplex, and broad, respectively.
TLC was performed on a precoated silica gel plate (Merck silica gel 60 F-254). Silica gel (WAKO- gel C-200 (75-150 μm)) was used for all column chromatography separations. All chemicals were reagent grade and were purchased from Sigma-Aldrich, Wako pure chemical industries, Ltd., Great Britain, Tokyo kasei kogyo Co., Ltd., Nacalai tesque, Inc., Watanabe Chemical Ind. Ltd., Maybridge pic, Lancaster Synthesis Ltd., Merck KgaA, Germany, or Kanto Chemical Co., Ltd.
All starting materials are commercially available or can be prepared using methods cited in the literature.
ASSAYS AND PHARMACOLOGICAL TESTS
The effect of the present compounds was examined by the following assays and pharmacological tests.
[Measurement of capsaicin-induced Ca2+ influx in the human VRl -transfected CHO cell line] (Assay 1)
(1) Establishment of the human VRl -CH01uc9aeq cell line
Human vanilloid receptor (hVRl) cDNA was cloned from libraries of axotomized dorsal root ganglia (WO 00/29577). The cloned hVRl cDNA was constructed with pcDNA3 vector and transfected into a CH01uc9aeq cell line. The cell line contains aequorin and CRE-luciferase reporter genes as read-out signals. The fransfectants were cloned by limiting dilution in selection medium (DMEM/F12 medium (Gibco BRL) supplemented with 10% FCS, 1.4 mM Sodium pyruvate, 20 mM HEPES, 0.15% Sodium bicarbonate, 100 U/ml penicillin, 100 μg/ml streptomycin, 2 mM glutamine, non-essential amino acids and 2 mg/ml G418). Ca2+ influx was examined in the capsaicin-stimulated clones. A high responder clone was selected and used for further experiments in the project. The human VRl-CH01uc9aeq cells were maintained in the selection medium and passaged every 3-4 days at l-2.5xl05 cells/flask (75 mm2).
(2) Measurement of Ca2+ influx using FDSS-3000
Human VRl-CH01uc9aeq cells were suspended in a culture medium which is the same as the selection medium except for G418 and seeded at a density of 1,000 cells per well into 384-well plates (black walled clear-base / Νalge Νunc International). Following the culture for 48 hrs the medium was changed to 2 μM Fluo-3 AM (Molecular Probes) and 0.02% Puronic F-127 in assay buffer (Hank's balanced salt solution (HBSS), 17 mM HEPES (ρH7.4), 1 mM Probenecid, 0.1% BSA) and the cells were incubated for 60 min at 25°C. After washing twice with assay buffer the cells were incubated with a test compound or vehicle for 20 min at 25°C. Mobilization of cytoplasmic Ca2+ was measured by FDSS-3000 (λex=488nm, λem=540nm / Hamamatsu Photonics) for 60 sec after the stimulation with 10 nM capsaicin. Integral R was calculated and compared with controls.
[Measurement of the capsaicin-induced Ca2+ influx in primary cultured rat dorsal root ganglia neurons] (Assay 2)
(1) Preparation of rat dorsal root ganglia neurons
New bom Wister rats (5-11 days) were sacrificed and dorsal root ganglia (DRG) was removed. DRG was incubated with 0.1% trypsin (Gibco BRL) in PBS(-) (Gibco BRL) for 30 min at 37°C, then a half volume of fetal calf serum (FCS) was added and the cells were spun down. The DRG neuron cells were resuspended in Ham F12/5% FCS/5% horse serum (Gibco BRL) and dispersed by repeated pipetting and passing through 70 μm mesh (Falcon). The culture plate was incubated for 3 hours at 37°C to remove contaminating Schwann cells. Νon-adherent cells were recovered and further cultured in laminin-coated 384 well plates (Νunc) at lxlO4 cells/50 μl well for 2 days in the presence of 50 ng/ml recombinant rat NGF (Sigma) and 50 μM 5-fluorodeoxyuridine (Sigma).
(2) Ca2+ mobilization assay
DRG neuron cells were washed twice with HBSS supplemented with 17 mM HEPES φH 7.4) and 0.1% BSA. After incubating with 2 μM fluo-3AM (Molecular Probe), 0.02% PF127 (Gibco BRL) and 1 mM probenecid (Sigma) for 40 min at 37°C, cells were washed 3 times. The cells were incubated with VRl antagonists or vehicle (dimethylsulfoxide) and then with 1 μM capsaicin in FDSS-6000 (λex=480nm, λem=520nm / Hamamatsu Photonics). The fluorescence changes at 480nm were monitored for 2.5 min. Integral R was calculated and compared with controls.
[Organ bath assay to measure the capsaicin-induced bladder contraction] (Assay 3)
Male Wistar rats (10 week old) were anesthetized with ether and sacrificed by dislocating the necks. The whole urinary bladder was excised and placed in oxygenated Modified Rrebs-Henseleit solution (pH 7.4) of the following composition (112 mM ΝaCl, 5.9 mM KCI, 1.2 mM MgCl2, 1.2 mM ΝaH2P0 , 2 mM CaCl2, 2.5 mM NaHC03, 12 mM glucose). Confractile responses of the urinary bladder were studied as described previously [Maggi CA et al: Br.J.Pharmacol. 108: 801- 805, 1993]. Isometric tension was recorded under a load of 1 g using longitudinal strips of rat detrusor muscle. Bladder strips were equilibrated for 60 min before each stimulation. Contractile response to 80 mM KCI was determined at 15 min intervals until reproducible responses were obtained. The response to KCI was used as an internal standard to evaluate the maximal response to capsaicin. The effects of the compounds were investigated by incubating the strips with compounds for 30 min prior to the stimulation with 1 μM capsaicin (vehicle: 80% saline, 10% EtOH, and 10% Tween 80). One of the preparations made from the same animal was served as a confrol while the others were used for evaluating compounds. Ratio of each capsaicin-induced confraction to the internal standard (i.e. KCl-induced contraction) was calculated and the effects of the test compounds on the capsaicin-induced confraction were evaluated.
[Measurement of Ca2+ influx in the human P2X1 -transfected CHO cell line]
(1) Preparation of the human P2X1 -transfected CH01uc9aeq cell line
Human P2X1 -transfected CH01uc9aeq cell line was established and maintained in Dulbecco's modified Eagle's medium (DMEM/F12) supplemented with 7.5% FCS, 20 mM HEPES-KOH (pH 7.4), 1.4 mM sodium pyruvate, 100 U/ml penicillin, 100 μg/ml streptomycin, 2 mM glutamine (Gibco BRL) and 0.5 Units/ml apyrase (grade I, Sigma). The suspended cells were seeded in each well of 384-well optical bottom black plates (Nalge Nunc International) at 3 x 103 / 50 μl / well. The cells were cultured for following 48 hrs to adhere to the plates.
(2) Measurement of the intracellular Ca2+ levels
P2X1 receptor agonist-mediated increases in cytosolic Ca2+ levels were measured using a fluorescent Ca2+ chelating dye, Fluo-3 AM (Molecular Probes). The plate-attached cells were washed twice with washing buffer (HBSS, 17 mM HEPES-KOH (pH 7.4), 0.1% BSA and 0.5 units/ml apyrase), and incubated in 40 μl of loading buffer (1 μM Fluo-3 AM, 1 mM probenecid, 1 μM cyclosporin A, 0.01% pluronic (Molecular Probes)in washing buffer) for 1 hour in a dark place. The plates were washed twice with 40 μl washing buffer and 35 μl of washing buffer were added in each well with 5 μl of test compounds or 2 ',3 '- o-(2,4,6-trinifrophenyl) adenosine 5'-triphpsphate (Molecular Probes) as a reference. After further incubation for 10 minutes in dark 200 nM α, β-methylene ATP agonist was added to initiate the Ca2+ mobilization. Fluorescence intensity was measured by FDSS-6000 (λex=410nm, λem=510nm / Hamamatsu Photonics) at 250 msec intervals. Integral ratios were calculated from the data and compared with that of a control.
[Measurement of capsaicin-induced bladder confraction in anesthetized rats] (Assay 4)
(1) Animals
Female Sprague-Dawley rats (200-250 g / Charles River Japan) were used. (2) Catheter implantation
Rats were anesthetized by intraperitoneal adminisfration of urethane (Sigma) at 1.2 g/kg. The abdomen was opened through a midline incision, and a polyethylene catheter (BECTON DICKINSON, PE50) was implanted into the bladder through the dome. In parallel, the inguinal region was incised, and a polyethylene catheter (Hibiki, size 5) filled with 2 IU / ml of heparin (Νovo Heparin, Aventis Pharma) in saline (Otsuka) was inserted into a common iliac artery.
(3) Cystometric investigation
The bladder catheter was connected via T-tube to a pressure transducer (Viggo- Spectramed Pte Ltd, DT-XXAD) and a microinjection pump (TERUMO). Saline was infused at room temperature into the bladder at a rate of 2.4 ml/hr. fritravesical pressure was recorded continuously on a chart pen recorder (Yokogawa). At least three reproducible mictarition cycles, conesponding to a 20-minute period, were recorded before a test compound administration and used as baseline values.
(4) Administration of test compounds and stimulation of bladder with capsaicin
The saline infusion was stopped before administrating compounds. A testing compound dissolved in the mixture of ethanol, Tween 80 (ICΝ Biomedicals Inc.) and saline (1 : 1 : 8, v/v/v) was administered mtraarterially at 10 mg/kg. 2min after the administration of the compound 10 μg of capsaicin (Νacalai Tesque) dissolved in ethanol was administered mtraarterially.
(5) Analysis of cystometry parameters
Relative increases in the capsaicin-induced intravesical pressure were analyzed from the cystometry data. The capsaicin-induced bladder pressures were compared with the maximum bladder pressure during mictarition without the capsaicin stimulation. The testing compounds-mediated inhibition of the increased bladder pressures was evaluated using Student's t-test. A probability level less than 5% was accepted as significant difference. [Measurement of over active bladder in anesthetized cystitis rats] (Assay 5)
(1) Animals
Female Sprague-Dawley rats (180-250 g / Charles River Japan) were used. Cyclo- phosphamide (CYP) dissolved in saline was administered infraperitoneally at 150 mg/kg 48 hours before experiment.
(2) Catheter implantation
Rats were anesthetized by intraperitoneal administration of urethane (Sigma) at 1.25 g/kg. The abdomen was opened through a midline incision, and a polyethylene catheter (BECTON DICKINSON PE50) was implanted into the bladder through the dome. In parallel, the inguinal region was incised, and a polyethylene catheter (BECTON DICKINSON, PE50) filled with saline (Otsuka) was inserted into a femoral vein. After the bladder was emptied, the rats were left for 1 hour for recovery from the operation.
(3) Cystometric investigation
The bladder catheter was connected via T-tabe to a pressure transducer (Viggo- Spectramed Pte Ltd, DT-XXAD) and a microinjection pump (TERUMO). Saline was infused at room temperature into the bladder at a rate of 3.6 ml/hr for 20 min. Intravesical pressure was recorded continuously on a chart pen recorder (Yokogawa). At least three reproducible micturition cycles, conesponding to a 20-minute period, were recorded before a test compound administration.
(4) Administration of test compounds
A testing compound dissolved in the mixture of ethanol, Tween 80 (ICN Biomedicals Inc.) and saline (1 : 1 : 8, v/v/v) was administered intravenously at 0.05 mg/kg, 0.5 mg/kg or 5 mg/kg. 3min after the adminisfration of the compound, saline (Nacalai Tesque) was infused at room temperatare into the bladder at a rate of 3.6 ml/hr.
(5) Analysis of cystometry parameters
The cystometry parameters were analyzed as described previously [ Lecci A et al: Eur. J. Pharmacol. 259: 129-135, 1994]. The micturition frequency calculated from micturition interval and the bladder capacity calculated from a volume of infused saline until the first mictarition were analyzed from the cystometry data. The testing compounds-mediated inhibition of the frequency and the testing compounds-mediated increase of bladder capacity were evaluated using unpaired Student's t-test. A probability levels less than 5% was accepted as significant difference. Data were analyzed as the mean ± SEM from 4 - 7 rats.
[Measurement of Acute Pain]
Acute pain is measured on a hot plate mainly in rats. Two variants of hot plate testing are used: In the classical variant animals are put on a hot surface (52 to 56 °C) and the latency time is measured until the animals show nociceptive behavior, such as stepping or foot licking. The other variant is an increasing temperature hot plate where the experimental animals are put on a surface of neutral temperature. Subsequently this surface is slowly but constantly heated until the animals begin to lick a hind paw. The temperature which is reached when hind paw licking begins is a measure for pain threshold.
Compounds are tested against a vehicle treated control group. Substance application is performed at different time points via different application routes (i.v., i.p., p.o., i. , i.c.v., s.c, intradermal, transdermal) prior to pain testing.
[Measurement of Persistent Pain]
Persistent pain is measured with the formalin or capsaicin test, mainly in rats. A solution of 1 to 5% formalin or 10 to 100 μg capsaicin is injected into one hind paw of the experimental animal. After formalin or capsaicin application the animals show nociceptive reactions like flinching, licking and biting of the affected paw. The number of nociceptive reactions within a time frame of up to 90 minutes is a measure for intensity of pain.
Compounds are tested against a vehicle treated control group. Substance application is performed at different time points via different application routes (i.v., i.p., p.o., i.t., i.c.v., s.c, intradermal, transdermal) prior to formalin or capsaicin administration.
[Measurement of Neuropathic Pain]
Neuropathic pain is induced by different variants of unilateral sciatic nerve injury mainly in rats. The operation is performed under anesthesia. The first variant of sciatic nerve injury is produced by placing loosely constrictive ligatures around the common sciatic nerve (Bennett and Xie, Pain 33 (1988): 87-107). The second variant is the tight ligation of about the half of the diameter of the common sciatic nerve (Seltzer et al., Pain 43 (1990): 205-218). In the next variant, a group of models is used in which tight ligations or fransections are made of either the L5 and L6 spinal nerves, or the L5 spinal nerve only (KIM SH; CHUNG JM, AN EXPERIMENTAL-MODEL FOR PERIPHERAL NEUROPATHY PRODUCED BY SEGMENTAL SPINAL NERVE LIGATION IN THE RA, PAIN 50 (3) (1992): 355-363). The fourth variant involves an axotomy of two of the three terminal branches of the sciatic nerve (tibial and common peroneal nerves) leaving the remaining sural nerve intact whereas the last variant comprises the axotomy of only the tibial branch leaving the sural and common nerves uninjured. Confrol animals are treated with a sham operation.
Postoperatively, the nerve injured animals develop a chronic mechanical allodynia, cold allodynia, as well as a thermal hyperalgesia. Mechanical allodynia is measured by means of a pressure transducer (electronic von Frey Anesthesiometer, HTC fric.-Life Science Instruments, Woodland Hills, SA, USA; Electronic von Frey System, Somedic Sales AB, Hδrby, Sweden). Thermal hyperalgesia is measured by means of a radiant heat source (Plantar Test, Ugo Basile, Comerio, Italy), or by means of a cold plate of 5 to 10°C where the nocifensive reactions of the affected hind paw are counted as a measure of pain intensity. A further test for cold induced pain is the counting of nocifensive reactions, or duration of nocifensive responses after plantar adminisfration of acetone to the affected hind limb. Chronic pain in general is assessed by registering the circadanian rhytms in activity (Surjo and Amdt, Universitat zu Kδln, Cologne, Germany), and by scoring differences in gait (foot print patterns; FOOTPPTNTS program, Klapdor et al., 1997. A low cost method to analyse footprint patterns. J. Neurosci. Methods 75, 49-54).
Compounds are tested against sham operated and vehicle treated control groups. Substance application is performed at different time points via different application routes (i.v., i.p., p.o., i.t., i.c.v., s.c, intradermal, transdermal) prior to pain testing.
[Measurement of Inflammatory Pain]
Inflammatory pain is induced mainly in rats by injection of 0.75 mg carrageenan or complete Freund's adjuvant into one hind paw. The animals develop an edema with mechanical allodynia as well as thermal hyperalgesia. Mechanical allodynia is measured by means of a pressure transducer (electronic von Frey Anesthesiometer, HTC Lie-Life Science Instruments, Woodland Hills, SA, USA). Thermal hyperalgesia is measured by means of a radiant heat source (Plantar Test, Ugo Basile, Comerio, Italy, Paw thermal stimulator, G. Ozaki, University of California, USA). For edema measurement two methods are being used. In the first method, the animals are sacrificed and the affected hindpaws sectioned and weighed. The second method comprises differences in paw volume by measuring water displacement in a plethysmometer (Ugo Basile, Comerio, Italy).
Compoxmds are tested against uninflamed as well as vehicle treated control groups. Substance application is performed at different time points via different application routes (i.v., i.p., p.o., i.t., i.c.v., s.c, intradermal, transdermal) prior to pain testing. [Measurement of Diabetic Neuropathic Pain]
Rats freated with a single intraperitoneal injection of 50 to 80 mg/kg sfreptozotocin develop a profound hyperglycemia and mechanical allodynia within 1 to 3 weeks. Mechanical allodynia is measured by means of a pressure transducer (electronic von Frey Anesthesiometer, HTC Lie-Life Science Instruments, Woodland Hills, SA, USA).
Compounds are tested against diabetic and non-diabetic vehicle treated control groups. Substance application is performed at different time points via different application routes (i.v., i.p., p.o., i.t., i.c.v., s.c, intradermal, transdermal) prior to pain testing.
Results in capsaicin-induced Ca2+ influx assay in the human VRl -transfected CHO cell line (Assay 1) are shown in Examples and tables of the Examples below. For practical reasons, the compounds are grouped in four classes based on activity as follows:
IC50= A (< or =) O.lμM < B (< or =) 0.5 μM < C (< or =) 1 μM < D
The compoxmds of the present invention also show excellent selectivity, and strong activity in other assays 2-5 and assays for pain described above.
CHAPTER I (EXAMPLES^)
Preparing method of starting compounds
4- Amino-2,3-dihydro-lH-inden-2-yl acetate
Figure imgf000076_0001
Ac20, Pyridine Toluene
Figure imgf000076_0002
To a solution of 2-nitrobenzyl bromide (1.00 g, 4.63 mmol) and diethyl malonate (0.741 g, 4.63 mmol) in 30 ml of hexane was added potassium carbonate (0.640 g, 4.63 mmol) and 18- Crown-6 (0.012 g, 0.05 mmol). After stined at 80 °C for 18 hours, the mixtare was diluted with water and was extracted with ethyl acetate. The organic layer was washed with water, then with brine, and concentrated under reduced pressure to obtain crude diethyl (2-nitrobenzyl)malonate.
A solution of crude diethyl (2-nifrobenzyl)malonate in 6N aqueous HCl (15 ml) and acetic acid (15 ml) was stined at refluxing temperature for 48 hours. After cooled to ambient temperature, the mixtare was concentrated under reduced pressure. To the residue was added 10% aqueous NaOH solution and washed with ethyl acetate. The aqueous layer was acidified with aqueous HCl solution, and the mixture was extracted with ethyl acetate. The organic layer was dried over MgS04, filtered, and concentrated under reduced pressure to obtain 3-(2-nitrophenyl)propanoic acid.
Η NMR (CDC13) δ 2.79 (t, J= 7.6 Hz, 2H), 3.24 (t, J= 7.6 Hz, 2H), 7.38-7.44 (m, 2H), 7.55 (dt, J= 7.6, 1.6 Hz, IH), 7.96 (dd, J= 7.6, 1.6 Hz, IH).
A solution of 3-(2-nitrophenyl)propanoic acid (1.20 g, 6.15 mmol) and thionyl chloride (0.878 g, 7.38 mmol) in dichloromethane (5 ml) was stined and heated to reflux for 2 hours. The mixture was concentrated under reduced pressure to obtain 3-(2-nitrophenyl)propanoyl chloride. To the obtained crude 3-(2-nitrophenyl)propanoyl chloride (1.31 g, 6.15 mmol) was added CS2, and aluminum trichloride (1.07 g, 8.0 mmol) was added portionwise at 0°C. The mixture was stined at 70°C for 3 hours, and after cooled to ambient temperatare, water was added and exfracted with ethyl acetate. The organic layer was dried over MgS0 , filtered, and concentrated under reduced pressure. The obtained residue was purified by silica gel column chromatography (hexane:ethylacetate 10:1) to afford 4-nifroindan-l-one (0.44 g).
JH NMR (CDC13) δ 2.79-2.82 (m, 2H), 3.64-3.66 (m, 2H), 7.62 (t, J= 7.9 Hz, IH), 8.09 (d, J= 7.6 Hz, IH), 8.47 (d, J= 8.2 Hz, IH).
To a solution of 4-nitroindan-l-one (0.381 g, 2.15 mmol) in ethanol (5 ml) was added sodium borohydride (0.048 g, 1.29 mmol) at 0 °C, and the mixture was stined at room temperatare for 3 hours. Aqueous solution of ammonium chloride was added to the mixture, and extracted with ethyl acetate. The organic layer was dried over MgS04, filtered, and concentrated under reduced pressure to obtain 4-nitroindan-l-ol.
Η NMR (CDC13) δ 1.90 (d, J= 6.5 Hz, IH), 2.00-2.07 (m, IH), 2.56-2.63 (m, IH), 3.25-3.33 (m, IH), 3.54-3.60 (m, IH), 5.30-5.35 (m, IH), 7.44 (t, J= 8.2 Hz, IH), 7.72 (d, J= 7.6 Hz, IH), 8.12 (d, J= 8.2 Hz, IH).
A solution of 4-nitroindan-l-ol (0.385 g, 2.15 mmol) and />-toiuenesulfonic acid (5.0 mg, 0.03 mmol) in toluene (30 ml) was stined and heated to reflux for 16 hours. After cooled to ambient temperature, the mixtare was washed with aqueous sodium bicarbonate solution. The organic layer was dried over MgS04, filtered, and concentrated under reduced pressure. The obtained residue was purified by preparatory TLC (hexane:ethylacetate 3:1) to afford 7-nifro-lH- indene (0.289 g).
JH NMR (CDC13) δ 3.94 (s, 2H), 6.75 (dt, J= 5.7, 1.9 Hz, IH), 6.93 (dt, J= 5.7, 1.6 Hz, IH), 7.45 (t, J= 8.2 Hz, IH), 7.68 (d, J= 7.6 Hz, IH), 8.05 (d, J= 8.2 Hz, IH). To a solution of 2,3-dimethyl-2-butene (21.5 mg, 0.31 mmol) in THF (2 ml) at 0°C was added borane-THF (0.307 ml, 0.31 mmol) dropwise. After stined for lhour at 0°C, 7-nitro-lH-indene (45.0 mg, 0.28 mmol) in THF (5 ml) was added dropwise, and the mixture was stined for 2 hours at ambient temperature. The mixture was cooled to 0°C, and water (0.15 ml), 4N aqueous sodium hydroxide (0.45 ml), and 30% H202 (0.45ml) were added. The mixture was then warmed to room temperature and poured into water, extracted with ethyl acetate and washed with brine. The organic layer was dried over MgS0 , filtered, and concentrated under reduced pressure. To the obtained mixture in toluene (1 ml) was added acetic anhydride (40.8 mg, 0.40 mmol) and pyridine (0.4 ml), and then stined for 16 hours at room temperature. The mixture was concentrated under reduced pressure, and the obtained residue was purified by preparatory TLC (hexane: ethylacetate 2:1) to obtain 4-nifro-2,3-dihydro-lH-inden-2-yl acetate (16.0 mg).
Η NMR (CDC13) δ 2.03 (s, 3H), 3.12 (dd, J= 17.5, 1.6 Hz, IH), 3.40 (dd, J= 17.5, 6.3 Hz, IH), 3.60 (dd, J= 19.2, 2.2 Hz, IH), 3.74 (dd, J= 19.2, 6.6 Hz, IH), 5.58-5.62 (m, IH), 7.39 (t, J= 7.9 Hz, IH), 7.54 (d, J= 7.3 Hz, IH), 8.06 (d, J= 8.2 Hz, IH).
To a mixture of 4-nitro-2,3-dihydro-lH-inden-2-yl acetate (100 mg, 0.45 mmol) and ammonium chloride (100 mg) in ethanol (6 ml) and water (3 ml) was added iron powder (300 mg) portionwise at room temperatare. The mixture was stined at 90 °C for 1 hour, and after cooled to room temperature, the mixture was diluted with ethylacetate. The mixture was filtered through a pad of celite, and the filtrate was washed with brine, dried over MgS04, filtered, and concentrated under reduced pressure to obtain 4-amino-2,3-dihydro-lH-inden-2-yl acetate.
*H NMR (CDC13) δ 2.03 (s, 3H), 2.81 (dd, J= 16.4, 2.8 Hz, IH), 3.00 (dd, J= 16.7, 2.8 Hz, IH), 3.14 (dd, J= 16.4, 6.6 Hz, IH), 3.29 (dd, J= 16.7, 6.6 Hz, IH), 3.58 (br.s, 2H), 5.51-5.56 (m, IH), 6.54 (d, J= 7.9 Hz, IH), 6.69 (d, J= 7.3 Hz, IH), 7.04 (t, J= 7.9 Hz, IH).
Example 1-1
4-[({[4-CMoro-3-(trifluoromethyl)phenyl]amino}carbonyl)amino]-2,3-dihydro-lH-inden-2-yl acetate
Figure imgf000079_0001
A mixture of 4-amino-2,3-dihydro-lH-inden-2-yl acetate (86.4 mg, 0.45 mmol) and 4-chloro-3- xrifluoromethylphenyl isocyanate (110 mg, 0.50 mmol) in 1,4-dioxane (2 m) was stined at 50°C for 15 hours. The mixture was concentrated under reduced pressure, and to the obtained residue was added diisopropyl ether. The precipitate was collected to afford 4-[({[4-chloro-3-(trifluoro- methyl)phenyl]amino}carbonyl)amino]-2,3-dihydro-lH-inden-2-yl acetate (128 mg).
Η NMR (DMSO-Je) δ 1.98 (s, 3H), 2.91 (ddd, J= 19.6, 17.1, 1.9 Hz, 2H), 3.21-3.30 (m, ITT), 5.40-5.45 (m, IH), 6.96 (d, J= 7.3 Hz, IH), 7.15 (t, J= 7.9 Hz, IH), 7.62 (s, 2H), 7.71 (d, J= 8.2 Hz, IH), 8.10 (s, IH), 8.25 (s, IH), 9.34 (s, IH);
Molecular weight : 412.80
MS (M+H): 413
Mp 207-209°C;
Activity class: C
L the similar manner as described in Example 1-1, compounds in Example 1-2 to 1-3 as shown in Table 1 were synthesized.
Table 1
Figure imgf000080_0002
Starting material
(6-Ethoxy-5,8-dihydronaphthalen-l-yl)amine
Figure imgf000080_0001
A mixture of 5-amino-2-naphthol (4.78 g, 30.0 mmol), benzaldehyde (3.50 g, 33.0 mmol), and magnesium sulfate (10.0 g) in THF (100 ml) was heated to reflux for 16 hours. After cooled to ambient temperature, the mixture was filtered through a pad of celite and the filtrate was concentrated under reduced pressure. The obtained residue was recrystallized with diethylether to afford 5-{[phenylmethylene]amino}-2-naphthol (7.40 g).
!H NMR (CDC13) δ 5.06 (br.s, IH), 6.92 (d, J= 6.6 Hz, IH), 7.10-7.17 (m, 2H), 7.42-7.49 (dd, J= 6.6 Hz, IH), 7.45-7.55 (m, 4H), 8.00-8.02 (m, TT), 8.27 (d, J= 9.0 Hz, IH), 8.56 (s, IH)
Molecular weight : 247.30
MS (M+H): 248
To a solution of 5-{(phenylmethylene)amino}-2-naphthol (2.00 g, 8.09 mmol) in DMF (50 ml) was added ethyl iodide (1.39 g, 8.90 mmol) at room temperature and stined at 50°C for 2 hours. After cooled to ambient temperatare, water was added and the mixture was exfracted with ethyl acetate. The organic layer was washed with water and brine, dried over MgS04, filtered, and concentrated under reduced pressure. The obtained residue was purified by silica gel column chromatography (hexanexthylacetate 15:1) to afford (6-ethoxy-l-naphthyl)(phenylmethylene)amine (1.54 g).
Η NMR (CDCI3) δ 1.49 (3H, t, J = 6.8 Hz), 4.17 (2H, q, J = 6.8 Hz), 6.91 (IH, dd, J = 1.1, 7.5 Hz), 7.14-7.18 (2H, m), 7.41 (IH, dd, J= 1.1, 7.2 Hz), 7.50-7.61 (4H, m), 7.99-8.03 (2H, m), 8.25 (IH, d, J= 8.7 Hz), 8.55 (IH, s);
Molecular weight : 275.35
MS (M+H): 276
A mixture of (6-ethoxy-l-naphthyl)(phenylmethylene)amine (0.600 g, 2.18 mmol) and Pd/C (0.900 g) in ethyl acetate (15 ml) was stined under argon at room temperatare for 48 hours. The mixtare was filtered through a pad of celite, and the filtrate was concentrated under reduced pressure. The obtained residue was purified by silica gel column chromatography (hexane: ethylacetate 4:1) to provide (6-ethoxy-l-naphthyl)amine (2.78 g).
Η NMR (CDC13) δ 1.46 (3H, t, J= 6.8 Hz), 4.06 (2H, brs), 4.13 (2H, q, J= 6.8 Hz), 6.62 (IH, dd, J= 1.5, 6.8 Hz), 7.08-7.12 (2H, m), 7.16-7.25 (2H, m), 7.70 (IH, d, J= 9.8 Hz)
Molecular weight : 187.24
MS (M+H): 188
To a mixture of (6-ethoxy-l-naphthyl)amine (300 mg, 1.60 mmol) and tert-buthanol (641 mg, 8.65 mmol) in THF (4 ml) and liquid ammonia (55 ml) at -78°C was added lithium (96.8 mg, 13.94 mmol) portionwise. After the mixture was stined for 30 minutes at -78°C, methanol (9 ml) and water were added. Ammonia was removed at room temperature, and the resulted mixture was extracted with ethyl acetate. The organic layer was dried over MgS0 , filtered, and concenfrated under reduced pressure. The obtained residue was purified by silica gel column chromatography (hexane:ethylacetate 4: 1) to afford (6-ethoxy-5,8-dihydronaphthalen-l-yl)amine (248 mg).
Η NMR (CDC13) δ 1.33 (3H, t, J= 6.8 Hz), 3.17 (IH, dd, J= 3.4, 5.1 Hz), 3.20 (IH, dd, J= 3.4, 5.1 Hz), 3.42 (IH, d, J= 5.1 Hz), 3.43 (IH, d, J= 5.1Hz), 3.57 (2H, brs), 3.81 (2H, q, J= 6.8 Hz), 4.77 (IH, t, J= 3.4 Hz), 6.52 (IH, d, J= 7.9 Hz), 6.58 (lH,d, J= 7.5 Hz), 6.98 (IH, dd, J= 7.5, 7.9 Hz).
Molecular weight : 189.26
MS (M+H): 190
Example 2-1
N-[4-ChIoro-3-(trifluoromethyl)phenyl]-N'-(6-ethoxy-5,8-dihydronaphthalen-l-yl)urea
Figure imgf000082_0001
Next, to a solution of (6-ethoxy-5,8-dihydronaphthalen-l-yl)amine (108 mg, 0.57 mmol) in THF (5 ml) was added 4-chloro-3-trifluoromethyl isocyanate (139 mg, 0.63 mmol), and the mixture was stined for 13 hours. Saturated aqueous solution of sodium carbonate was added and the mixture was extracted with ethylacetate. The organic layer was dried over MgS04, filtered, and concentrated under reduced pressure to obtain N-[4-chloro-3-(trifluoromethyl)phenyl]-N'-(6- ethoxy-5,8-dihydronaphthalen-l-yl)urea (234 mg).
Η NMR (DMSO-Jβ) δ 1.26 (3H, t, J= 6.8 Hz), 3.29-3.38 (4H, m), 3.80 (2H, q, J= 6.8 Hz), 6.91 (IH, d, J = 7.5 Hz), 7.14 (IH, dd, J= 7.5, 7.9 Hz), 7.59-7.61 (2H, m), 8.01 (lH,s), 8.10 (IH, s), 9.45 (lH,s)
Molecular weight : 410.82 MS (M+H): 411
Mp 216°C;
Activity class: B
Example 2-2
N- [4-Chloro-3-(trifluoromethyl)phenyl] -N'-(6-oxo-5,6,7,8-tetr ahydronaphthalen-l-yϊ)urea
Figure imgf000083_0001
To a solution of N-[4-chloro-3-(trifluoromethyl)phenyl]-N'-(6-ethoxy-5,8-dihydronaphthalen-l-yl)- urea (50.0 mg, 0.12 mmol) was added aqueous IN HCl solution at room temperatare. After stined for 20 minutes, satarated aqueous solution of sodium carbonate was added and the mixture was extracted with ethylacetate. The organic layer was dried over MgS04, filtered, and concentrated under reduced pressure. The obtained residue was purified by silica gel column chromatography (hexane:ethylacetate 1:2) to afford N-[4-chloro-3-(trifluoromethyl)phenyl]-N'-(6-oxo-5,6,7,8- tefrahydronaphthalen-l-yl)urea (41.7 mg).
Η NMR (Acetone-J6) δ 2.44 (2H, t, J = 6.4 Hz), 3.06 (2H, t, J = 6.4 Hz), 3.57 (2H, s), 6.98 (lH.d, J = 7.2 Hz), 7.19 (IH, dd, J= 7.2, 7.5 Hz), 7.51-7.52 (2H, m), 7.74 (IH, dd, J= 2.6, 8.7 Hz), 7.87 (IH, brs), 7.14 (IH, d, J= 2.6 Hz), 8.69 (, IH, brs);
Molecular weight : 382.77
MS (M+H): 383
Mp 219°C;
Activity class: A Example 2-3
N-[4-Chloro-3-(trifluoromethyI)phenyl]-N'-(6-hydroxy-5,6,7,8-tetrahydronaphthalen-l- yl)urea
Figure imgf000084_0001
To a solution of N-[4-chloro-3-(trifluoromethyl)phenyl]-N'-(6-oxo-5,6,7,8-tefrahydronaphthalen-l- yl)urea (70.0 mg, 0.18 mmol) in methanol (3 ml) was added sodium borohydride (7.61 mg, 0.20 mmol) at 0 °C. After stined for 30 minutes, the mixture was concentrated under reduced pressure and water was added. The mixture was extracted with ethylacetate, and the organic layer was dried over MgS0 , filtered, and concentrated under reduced pressure to obtain N-[4-chloro-3- (trifluoromethyl)phenyl]-N-(6-hydroxy-5,6,7,8-tetrahydronaphthalen-l-yl)urea (70.0 mg).
Η NMR (Acetone-^) δ 1.75 (lH,m), 2.04 (IH, m), 2.59-3.04 (4H, m), 4.02 (IH, m), 6.84 (IH, d, J= 7.2 Hz), 7.09 (IH, dd, J= 7.2, 7.5 Hz), 7.50-7.53 (2H, m). 7.67 (IH, d, J= 7.5 Hz), 7.72 (IH, dd, J= 2.6, 8.7 Hz), 8.13 (IH, d, J= 2.6 Hz), 8.77 (IH, s);
Molecular weight : 384.79
MS (M+H): 385
Mp 216°C
Activity class: A
Li the similar manner as described in Example 2-1, 2-2, or 2-3, compounds in Example 2-4 to 2-9 as shown in Table 2 were synthesized.
Figure imgf000085_0001
Figure imgf000086_0001
Starting material
(7-Methyl-5,6,7,8-tetrahydronaphthalen-l-yl)amine
Figure imgf000086_0002
A mixture of 8-amino-3,4-dihydronaphthalen-2(lH)-one (1.61 g, 9.99 mmol), benzyl bromide (1.88 g, 11.0 mmol), and potassium carbonate (2.07 g, 15.0 mmol) in acetone (50 mL) was stined at refluxing temperature for 16 hours. After the mixture was cooled to ambient temperatare, it was filtered through a pad of Celite, and the filfrate was concentrated under reduced pressure. The obtained residue was purified by silica gel column chromatography (eluent: ethylacetate / hexane = 1 / 10) to provide 8-(benzylamino)-3,4-dihydronaphthalen-2(lH)-one (1.87 g).
Η NMR (CDC13) δ 2.65 (dd, J= 12.9 Hz, 6.6 Hz, 2H), 3.10 (dd, J= 12.9 Hz, 6.6 Hz, 2H), 3.28 (s, 2H), 3.71 (brs, IH), 4.31 (s, 2H), 6.55 (d, J= 8.1 Hz, IH), 6.60 (d, J= 8.1 Hz, IH), 7.12 (t, J= 8.1 Hz, IH), 7.23 - 7.40 (m, 5H);
Molecular weight : 251.33
MS (M+H): 252 To a suspension of methyltriphenylphosphonium iodide (2.12 g, 5.25 mmol) in tetrahydrofuran (100 ml) was added sodium tert-butoxide (0.56 g, 5.83 mmol) at 0°C. After the mixtare was stined for 30 minutes, a solution of 8-(benzylamino)-3,4-dihydronaphthalen-2(lH)-one (0.66 g, 2.63 mmol) in tetrahydrofuran (10 ml) was added at room temperature and then stined at 100 °C for 13 hours. The mixtare was cooled to ambient temperatare and was poured into water. The mixture was extracted with ethylacetate, and the organic layer was dried over Na2S0 , filtered, and concentrated under reduced pressure to obtain N-benzyl-7-methylene-5,6,7,8-tetrahydro- naphthalen-1 -amine (0.367 g).
Η NMR (CDC13) δ 2.45 (dd, J= 12.9 Hz, 6.6 Hz, 2H), 2.86 (dd, 7= 12.9 Hz, 6.6 Hz, 2H), 3.20 (s, 2H), 3.80 (brs, IH), 4.37 (s, 2H), 4.86 - 4.90 (m, ITT), 6.50 (d, J= 8.1 Hz, IH), 6.55 (d, J= 8.1 Hz, IH), 7.05 (t, J= 8.1 Hz, IH), 7.29 - 7.41 (m, 5H);
Molecular weight : 249.36
MS (M+H): 250
To a solution of N-benzyl-7-methylene-5,6,7,8-tetrahydronaphthalen-l -amine (0.50 g, 2.00 mmol) in tetrahydrofuran (5 ml) was added 0.5 M tetrahydrofuran solution of 9-borabicyclo[3.3.1]nonane dimer (8.20 ml, 4.10 mmol) at 0°C and then stined at room temperature for 8 hours. To the resulting mixtare was added 3N aqueous solution of sodium hydroxide 82 ml) followed by aqueous 33% hydrogen peroxide solution (2 ml), and the mixtare was stined at room temperatare for 6 hours. The mixtare was extracted with ethylacetate, and the organic layer was washed with brine, dried over Na2S04, filtered, and concentrated under reduced pressure. The obtained residue was purified by silica gel column chromatography (eluent: ethylacetate / hexane = 1 / 4) to provide N-benzyl-7-methyl-5,6,7,8-tetrahydronaphthalen-l-amine (0.069 g).
Η NMR (CDC13) δ 1.11 (d, J= 6.9 Hz, 3H), 1.22 - 1.24 (m, IH), 1.55 - 1.65 (m, IH), 1.80 - 2.05 (m, 2H), 2.74 - 2.76 (m, 2H), 4.36 (s, 2H), 4.45 (brs, IH), 5.50 (brs, IH), 6.49 (m, 2H), 7.05 (t, J= 9.0 Hz, IH), 7.28 - 7.40 (m, 5H).
A mixture of N-benzyl-7-methyl-5,6,7,8-tefrahydronaphthalen-l-amine (90.0 mg, 0.346 mmol) and palladium carbon (10.0 mg) in ethylacetate (10 ml) was stined under hydrogen for 1 hour. The mixture was filtered through a pad of celite, and the filfrate was concentrated under reduced pressure. The obtained residue was purified by column chromatography (eluent: ethylacetate / hexane = 1 / 3) to provide (7-methyl-5,6,7,8-tetrahydronaphthalen-l-yl)amine (46.0 mg).
Η NMR (CDC13) δ 1.05 (d, J= 6.0 Hz, 3H), 1.09 - 1.19 (m, IH), 1.46 - 1.84 (m, 3H), 2.63- 2.69 (m, 2H), 4.25 (brs, 2H), 4.36 (brs, IH), 6.45 - 6.49 (m, 2H), 6.93 (t, J= 6.0 Hz, IH); Molecular weight : 161.25
MS (M+H): 162
Example 3-1
N-[4-Chloro-3-(trifluoromethyl)phenyl]-N'-(7-methyl-5,6,7,8-tetrahydronaphthalen-l- yl)urea
Figure imgf000088_0001
A mixture of (7-methyl-5,6,7,8-tetrahydronaphthalen-l-yl)amine (30.0 mg, 0.186 mmol) and 4- chloro-3-trifluoromethyl isocyanate (50.0 mg, 0.220 mmol) in tetrahydrofuran (10 ml) was stined at room temperature for 16 hours. After the mixture was concenfrated under reduced pressure, the obtained residue was purified by silica gel column chromatography (eluent: ethylacetate / hexane = 1 / 3) to provide N-[4-chloro-3-(trifluoromethyl)phenyl]-N'-(7-methyl-5,6,7,8-tefrahydro- naphthalen-l-yl)urea (42.0 mg).
TJ NMR (MeOD-da) δ 1.10 (d, J= 6.0 Hz, 3H), 1.35 - 1.67 (m, IH), 1.70 - 1.92 (m, IH), 1.93 - 2.15 (m, 3H), 2.61 - 2.70 (m, 2H), 3.88 - 3.92 (m, IH), 4.39 (d, J= 6.0 Hz, IH), 6.86 (d, J= 9.0 Hz, IH), 7.15 (d, J= 9.0 Hz, IH), 7.48 (d, J= 9.0 Hz, IH), 7.59 - 7.66 (m, 2H), 8.00 (s, IH).
Molecular weight : 382.82
MS (M+H): 383
Activity Class : A
Starting material
(8-Amino-l,2,3,4-tetrahydronaphthalen-2-yl)methanol
Figure imgf000089_0001
Figure imgf000089_0002
To a solution of 8-amino-3,4-dihydronaphthalen-2(lH)-one (5.00 g, 31.0 mmol) and pyridine (3.68 g, 46.5 mmol) in tetrahydrofuran (60 ml) was added benzyl chloroformate (6.35 g, 37.2 mmol) at 0°C. After the mixture was stined at room temperature for 1 hour, it was poured into water and extracted with ethylacetate. The organic layer was washed with brine, dried over MgS0 , filtered, and concentrated under reduced pressure. The obtained residue was washed with diethylether to provide benzyl (7-oxo-5,6,7,8-tefrahydronaphthalen-l-yl)carbamate (6.52 g).
Η NMR (CDCU) δ 2.58 (t, J = 6.8 Hz, 2H), 3.08 (t, J = 6.8 Hz, 2H), 3.47 (s, 2H), 5.19 (s, 2H), 6.37 (brs, IH), 7.07 (d, J= 7.3 Hz, IH), 7.22 (t, J= 7.9 Hz, IH), 7.33 - 7.50 (m, 6H).
To 2.6 M solution of n-butyllithium in hexane (1.72 ml) cooled at 0 C was added diisopropylamine (452 mg, 4.47 mmol) dropwise. After the mixture was stined at room temperatare for 15 minutes, a solution of benzyl (7-oxo-5,6,7,8-tefrahydronaphthalen-l-yl)carbamate (600 mg, 2.03 mmol) in tetrahydrofuran (1 ml) at -78°C and stined for 1 hour. A solution of methoxymethyl(diphenyl)- phosphine (550 mg, 2.23 mmol) in tetrahydrofuran (1 mL) was added to the reaction mixture at - 78°C and then stined for 16 hours at room temperature. The resulting mixtare was poured into water and extracted with ethylacetate. The organic layer was washed with brine, dried over MgS04, filtered, and concentrated under reduced pressure. The obtained residue was purified by silica gel column chromatography (eluent: hexane / ethylacetate = 10 / 1) to provide benzyl [(7E)- 7-(methoxymethylene)-5,6,7,8-tefrahydronaphthalen-l-yl]carbamate (109 mg). Η NMR (CDC13) δ 2.25 (t, J= 6.3 Hz, 2H), 2.79 (t, J= 6.3 Hz, ITT), 3.33 (s, 2H), 3.60 (s, 3H),
5.21 (s, 2H), 5.95 (s, IH), 6.49 (brs, IH), 6.85 (d, J= 7.6 Hz, IH), 7.12 (t, J= 7.9 Hz, IH), 7.33 -
7.43 (m, 5H), 7.73 (brs, IH).
A solution of benzyl [(7E)-7-(methoxymethylene)-5,6,7,8-tetrahydronaphthalen-l-yl]carbamate (51.0 mg, 0.16 mmol) in a mixture of tetrahydrofuran (3 ml) and 2N aqueous HCl (6 ml) was stined at room temperature for 2 hours, and then extracted with ethylacetate. The organic layer was washed with brine, dried over MgS04, filtered, and concentrated under reduced pressure. The obtained residue was dissolved in ethanol (2 ml) and sodium borohydride (5.97 mg, 0.16 mmol) was added at room temperature. After stined for 2 hours, the mixture was poured into water and exfracted with diethylether. The organic layer was washed with brine, dried over MgS04, filtered, and concentrated under reduced pressure. The obtained residue was purified by silica gel column chromatography (eluent: hexane / ethylacetate = 4 / 1) to provide benzyl [7-(hydroxymethyl)- 5,6,7,8-tetrahydronaphthalen-l-yl]carbamate (34.0 mg).
Η NMR (CDC13) δ 1.39 (m, IH), 1.48 (brs, IH), 1.95 - 1.98 (m, ITT), 2.24 (dd, J= 16.1 Hz, 10.1 Hz, IH), 2.72 (dd, J= 16.1 Hz, 5.2 Hz, IH), 2.77 - 2.88 (m, 3H), 3.63 - 3.65 (m, 2H), 5.20 (s, ITT), 6.90 (d, J= 7.6 Hz, IH), 7.13 (d, J= 7.7 Hz, IH), 7.32 - 7.42 (m, 5H), 7.62 (brs, IH).
A mixtare of benzyl [7-(hydroxymethyl)-5,6,7,8-tetrahydronaphthalen-l-yl]carbamate (32.0 mg, 0.10 mmol) and palladium carbon (30 mg) in ethanol (2 ml) was stined under hydrogen at room temperatare for 16 hours. The resulting mixtare was filtered through a pad of celite, and the filfrate was concentrated under reduced pressure. The obtained residue was purified by silica gel column chromatography (eluent: hexane / ethylacetate = 4 / 1) to provide (8-amino-l,2,3,4- tefrahydronaphthalen-2-yl)methanol (11.0 mg).
Η NMR (CDCI3) δ 1.37 - 1.44 (m, 2H), 1.94 - 2.04 (m, 2H), 2.14 (dd, J= 16.1 Hz, 10.4 Hz, IH), 2.64 (dd, J= 15.7 Hz, 15.3 Hz, IH), 2.79 - 2.82 (m, 2H), 3.58 (brs, 2H), 3.69 (d, J= 15.3 Hz, 2H), 6.53 (d, J= 7.9 Hz, IH), 6.57 (d, J= 7.5 Hz, IH), 6.95 (t, J= 15.3 Hz, IH). Example 4-1
N-[4-Chloro-3-(trifluoromethyl)phenyl]-N'-[7-(hydroxymethyl)-5,6,7,8-tetrahydro- naphthalen-l-yl]urea
Figure imgf000091_0001
A mixtare of (8-amino-l,2,3,4-tetrahydronaphthalen-2-yl)methanol (11.0 mg, 0.06 mmol) and 4- chloro-3-xrifluoromethylphenyl isocyanate (13.7 mg, 0.06 mmol) in 1,4-dioxane (2 ml) was stined for 2 hours at 50°C. The resulting mixtare was concentrated under reduced pressure, and the obtained residue was washed with diisopropyl ether to provide N-[4-chloro-3-(trifluoro- methyl)phenyl]-N'-[7-(hydroxymethyl)-5,6,7,8-tetrahydronaphthalen-l-yl]urea (14.0 mg).
Η NMR DMSO-d6) δ. 1.31 (m, IH), 1.75-1.83 (m, IH), 1.85 - 1.91 (m, IH), 2.21 (dd, J= 16.4 Hz, 10.4 Hz, IH), 2.70-2.81 (m, 3H), 3.44 (t, J= 5.7 Hz, IH), 4.67 (t, J= 5.1 Hz, IH), 6.83 (d, J = 7.5 Hz, IH), 7.05 (t, J= 7.8 Hz, IH), 7.56 - 7.64 (m, 3H), 7.94 (s, IH), 8.10 (d, J= 2.2 Hz, IH), 9.49 (s, IH).
mp l94 - 196°C;
Molecular weight : 398.81
MS (M+H): 399
Activity Class : A
CHAPTER A (EXAMPLES')
Preparing method of starting compounds
[Starting compound A]
7-ethoxy-5,8-dihydronaphthaIen-l-ylamine
Figure imgf000092_0001
To a stined solution of 8-amino-2-naphthol (50.0 g, 314 mmol) in tetrahydrofuran (1000 mL) was added di-t-butyldicarbonate (68.6 g, 314 mmol). The mixtare was stined at 70°C for 18 hours. After the mixture was cooled to room temperatare, solvent was removed under reduced pressure.
To the residue was added ethylacetate, and washed with saturated aqueous solution of sodium carbonate and then with water. The extracted organic layer was dried over Na2S04, filtered, and concentrated under reduced pressure. To the obtained residue was added diisopropyl ether, and the precipitate was filtered and dried to afford N-t-butoxycarbonyl-8-amino-2-naphthol (64.2 g, 79 % yield).
Next, to a mixture of N-t-butoxycarbonyl-8-amino-2-naphthol (64.0 g, 247 mmol) and Cesium carbonate (161 g, 493 mmol) in 300 mL anhydrous DMF was added iodoethane (42.3 g, 272 mmol) at room temperature. The mixture was stined at 60°C for 2 hours. Water was added to the mixture, and the product was exfracted with ethylacetate. The organic layer was washed with water and brine, dried over Na2S04, filtered, and concentrated under reduced pressure. To the obtained residue was added diisopropyl ether and the precipitate was collected and dried to afford (7-ethoxy-naphthalen-l-yl)-carbamic acid t-butyl ester (47.9 g, 67.5 % yield).
Next, to a (7-ethoxy-naphthalen-l-yl)-carbamic acid t-butyl ester (47.9 g, 167 mmol) in 100 mL anhydrous 1,4-dioxane was added 4N HCl in 1,4-dioxane (100 mL) at 0°C. The mixtare was stined at room temperature for 2 hours. Diisopropyl ether was added to the reaction mixture and the precipitate was filtered. To the obtained solid was added saturated sodium bicarbonate and the product was extracted with ethylacetate. The organic layer was dried over Na2S04, filtered, and concentrated under reduced pressure to afford 7-ethoxy-naphthalen-l-ylamine (27.0 g, 86.3 % yield).
Next, to a flask containing a mixture of 7-ethoxy-naphthalen-l-ylamine (1.80 g, 9.61 mmol) and t- buthanol (2.13 g, 28.8 mmol) in tetrahydrofuran (20 mL) was collected liquid ammonia (300 mL) at -78°C. To the mixture was added lithium (0.200 g, 28.8 mmol) over 30 minutes and stined at - 78°C for 1 hour. Methanol and water was added, and the mixture was stined at room temperature for 16 hours to allow ammonia to evaporate. To the obtained residue was added ethylacetate. The organic layer was washed with water, dried over Na2S04, filtered, and concenfrated under reduced pressure to afford 7-ethoxy-5,8-dihydronaphthalen-l-ylamine (1.37 g, 76 % yield).
[Starting compound B]
8-amino-1.2,3,4-tetrahydro-naphthalen-2-ol
Figure imgf000093_0001
To a stined solution of 7-ethoxy-5,8-dihydronaphthalen-l-ylamine (1.07 g, 5.65 mmol) in tetra- hydrofuran (30 mL) was added solution of aqueous 2N HCl (10 mL), and stiired at 40°C for 1 hour. The mixture was neutralized with addition of sodium bicorbonate, and the product was extracted with ethylacetate. The organic layer was washed with water, dried over Na2S04, filtered, and concentrated under reduced pressure to afford 8-amino-3,4-dihydro-lH-naphthalen-2-one (0.71 g, 78 % yield).
Next, to 8-amino-3,4-dihydro-lH-naphthalen-2-one (0.050 g, 0.318 mmol) in methanol (10 mL) was added sodium borohydride (0.030 g, 0.175 mmol) at 0°C, and the mixtare was stined for 1 hour. The mixture was poured into water, and the product was extracted with ethylacetate. The organic layer was dried over Na2S04, filtered, and concentrated under reduced pressure to afford 8- amino-l,2,3,4-tetrahydro-naphthalen-2-ol (0.037 g, 71 % yield). [Starting compound C]
8-Amino-l,2,3,4-tetrahydro-naphthalen-2-ol (enantiomer)
Figure imgf000094_0001
To a stined solution of benzeneruthenium(H) chloride dimer (3.10 mg, 0.006 mmol) and (IS, 2R)- (-)-cis-l-amino-2-indanol (3.7 mg, 0.025 mmol) in degaussed isopropanol was heated at 80°C for 20 minutes under argon. The mixture was added to the solution of 8-amino-3,4-dihydro-lH- naphthalen-2-one (50 mg, 0.310 mmol) in isopropanol (3 mL) at room temperature. A solution of potassium hydroxide (3.48 mg, 0.062 mmol) in isopropanol (1 mL) was added, and the mixtare was stiired at 45°C for 1 hour. The mixture was passed through silica gel and washed with ethylacetate. The filtrate was concentrated under reduced pressure to afford 8-amino-l,2,3,4- tefrahydro-naphthalen-2-ol enantiomer (33.0 mg, 65 % yield).
The other enantiomer of 8-amino-l,2,3,4-tetrahydronaphthalen-2-ol was obtained in the same fashion replacing (lS,2R)-(-)-cis-l-amino-2-indanol with (lR,2S)-(+)-cis-l-amino-2-indanol.
[Starting compound D]
(7-hydroxy-5,6,7,8-tetrahydro-naphthalen-l-yl)-carbamic acid phenyl ester
Figure imgf000094_0002
To a stined solution of 8-amino-l,2,3,4-tefrahydro-naphthalen-2-ol (30.0 mg, 0.18 mmol) and pyridine (21.8 mg, 0.28 mmol) in 1.0 mL THF was added phenyl chloroformate (30.2 mg, 0.19 mmol), and the mixtare was stined for 1 hour at room temperature. To the product mixture was added water and extracted with ethylacetate. The organic layer was washed with brine, dried over Na2S04, filtered and concentrated under reduced pressure. The obtained residue was triturated with ethylacetate and hexane to afford (7-hydroxy-5,6,7,8-tefrahydro-naphfhalen-l-yl)- carbamic acid phenyl ester (25.2 mg, 48 % yield). Example 1-1
N-l,3-benzodioxol-5-yl-N'-(7-hydroxy-5,6,7,8-tetrahydronaphthaIen-l-yl)urea
Figure imgf000095_0001
To a solution of phenyl l,3-benzodioxol-5-ylcarbamate (51.5 mg, 0.20 mmol) in dimethylsulfoxide (1 mL) was added 8-amino-l,2,3,4-tefrahydronaphthalen-2-ol (32.6 mg, 0.20 mmol) at room temperature. The mixture was stined at 100°C for 1.5 hours, then the mixtare was concenfrated under reduced pressure. The resulting residue was purified by preparatory TLC (hexane / ethylacetate = 1 / 1) to obtain N-1, 3-benzodioxol-5-yl-N-(7-hydroxy-5,6,7,8-tetrahydronaphthalen- l-yl)urea (7.10 mg).
TJ NMR (DMSO-Je) δ 1.55-1.66 (m, IH), 1.82-1.94 (m, IH), 2.38 (dd, J = 16.8, 8.1 Hz, IH), 2.79-2.91 (m, 3H), 3.89-3.99 (m, IH), 4.88 (d, J= 4.2 Hz, IH), 5.96 (s, 2H), 6.73 (dd, J= 2.1, 8.4 Hz, IH), 6.77 (d, J= 7.8 Hz, IH), 6.83 (d, J= 8.4 Hz, IH), 7.03 (t, J= 8.1 Hz, IH), 7.22 (d, J= 2.1 Hz, IH), 7.64 (d, J= 7.8 Hz, IH), 7.72 (s, IH), 8.93 (s, IH);
Molecular weight : 326.36
MS (M+H) : 327
Mp 209-211°C;
Activity grade: C
Li the similar manner as described in Example 1-1, compounds in Example 1-2 to 1- 13 as shown in Table 1 were synthesized. Table 1
Figure imgf000096_0001
Figure imgf000097_0001
Figure imgf000098_0001
Starting material l-[2,2-difluoro-l,3-benzodioxol-5-yl]methanamine
Figure imgf000098_0002
2,2-Difluoro-l,3-benzodioxole-5-carbonitrile (1000 mg, 5.46 mol) in ethanol (100 ml) is freated in the presence of Pd/C (200 mg) under a hydrogen atmosphere of 3 bar for lh. The catalyst is filtered off. The solvent is removed under reduced pressure and the crade mixture is treated with diethyl ether. The resulting crystals are separated from the solvent via a glass filter.
Yield: 650 mg (64 %) 'H NMR (300 MHz, DMSO-d6) δ 3.79 (s, 2H), 7.19 (d, IH), 7.35 (d, IH), 7.42 (s, IH).
LC-MS (EST): 188 (M+H)+; Retention time: 0.93 min (methode C)
Example 2-1
N-{[2,2-difluoro-l,3-benzodioxol-5-yl]methyl}-N'-[(7R)-7-hydroxy-5,6,7,8-tetrahydronaphthalen- l-yl]urea
Figure imgf000099_0001
Phenyl-[(7R)-7-hydroxy-5,6,7,8-tefrahydronaphthalen-l-yl]carbamate (100 mg, 0.35 mmol) and 1- [2,2-difluoro-l,3-benzodioxol-5-yl]methanamine (66 mg, 0.35 mmol) are dissolved in dimethylsulfoxide (2.00 ml) and stined at room temperatare for lh. The raw material is purified via HPLC.
Yield: 47 mg (35 %)
'H NMR (300 MHz, DMSO-d6) δ 1.52-1.64 (m, IH), 1.84-1.89 (m, IH), 2.34 (dd, IH), 2.64-2.87 (m, 3H), 3.91-3.92 (m, IH), 4.29 (d, 2H), 4.82 (d, IH), 6.72 (d, IH), 6.98 (t, IH), 7.05 (t, IH), 7.15 (dd, IH), 7.33-7.37 (m, 2H), 7.60-7.62 (m, 2H).
LC-MS (ESI*): 377.1 (M+H)+; Retention time: 2.00 min (method C)
Example 2-2
N-{[3-chloro-5-(frifluoromethyl)ρyridin-2-yl]methyl}-N'-[(7R)-7-hydroxy-5,6,7,8-tetrahydro- naphthalen- 1 -yl]urea
Figure imgf000099_0002
Phenyl-[(7R)-7-hydroxy-5,6,7,8-tefrahydronaphthalen-l-yl]carbamate (300 mg, 1.06 mmol), l-[3- chloro-5-(trifluoromethyl)pyridin-2-yl]methanamine hydrochloride (261 mg, 1.06 mmol) and N,Ν- diisopropylethylamine (191 mg, 1.48 mmol) are dissolved in dimethylsulfoxide (2.00 ml). The mixture is reacted at 60 °C for 3h, partitioned between ethyl acetate and water, the organic layer is dried over magnesium sulfate and evaporated to dryness in vacuo. The raw material is triturated with diethyl ether, filtered and dried.
Yield: 347 mg (82 %) Η NMR (200 MHz, DMSO-d6) δ 1.45-1.68 (m, IH), 1.78-1.95 (m, IH), 2.27-2.95 (m, 4H), 3.82- 4.03 (m, IH), 4.62 (d, 2H), 4.86 (d, IH), 6.72 (d, IH), 6.98 (t, IH), 7.28 (t, IH), 7.60 (d, IH), 7.95 (s, IH), 8.48 (d, IH), 8.93 (d, IH).
MS (EST): 400.1 (M+H)+
HPLC: Retention time 4.1 min (method B)
CHAPTER HI (EXAMPLES')
Preparing method of starting compounds
[Starting compound A]
7-ethoxy-5,8-dihydronaphthaIen-l-ylamine
Figure imgf000100_0001
To a stined solution of 8-amino-2-naphthol (50.0 g, 314 mmol) in tetrahydrofuran (1000 mL) was added di-t-butyldicarbohate (68.6 g, 314 mmol). The mixtare was stined at 70°C for 18 hours. After the mixture was cooled to room temperatare, solvent was removed under reduced pressure. To the residue was added ethylacetate, and washed with saturated aqueous solution of sodium carbonate and then with water. The extracted organic layer was dried over Na2S04, filtered, and concentrated under reduced pressure. To the obtained residue was added diisopropyl ether, and the precipitate was filtered and dried to afford N-t-butoxycarbonyl-8-amino-2-naphthol (64.2 g, 79 % yield).
Next, to a mixture of N-t-butoxycarbonyl-8-amino-2-naphthol (64.0 g, 247 mmol) and Cesium carbonate (161 g, 493 mmol) in 300 mL anhydrous DMF was added iodoethane (42.3 g, 272 mmol) at room temperature. The mixture was stined at 60°C for 2 hours. Water was added to the mixture, and the product was extracted with ethylacetate. The organic layer was washed with water and brine, dried over Na2S04, filtered, and concenfrated under reduced pressure. To the obtained residue was added diisopropyl ether and the precipitate was collected and dried to afford (7-ethoxy-naphthalen-l-yl)-carbamic acid t-butyl ester (47.9 g, 67.5 % yield).
Next, to a (7-ethoxy-naphthalen-l-yl)-carbamic acid t-butyl ester (47.9 g, 167 mmol) in 100 mL anhydrous 1,4-dioxane was added 4N HCl in 1,4-dioxane (100 mL) at 0°C. The mixtare was stined at room temperature for 2 hours. Diisopropyl ether was added to the reaction mixture and the precipitate was filtered. To the obtained solid was added saturated sodium bicarbonate and the product was exfracted with ethylacetate. The organic layer was dried over Na2S0 , filtered, and concentrated under reduced pressure to afford 7-ethoxy-naphthalen-l-ylamine (27.0 g, 86.3 % yield).
Next, to a flask containing a mixture of 7-ethoxy-naphthalen-l-ylamine (1.80 g, 9.61 mmol) and t- buthanol (2.13 g, 28.8 mmol) in tetrahydrofuran (20 mL) was collected liquid ammonia (300 mL) at -78°C. To the mixture was added lithium (0.200 g, 28.8 mmol) over 30 minutes and stined at - 78°C for 1 hour. Methanol and water was added, and the mixture was stined at room temperatare for 16 hours to allow ammonia to evaporate. To the obtained residue was added ethylacetate. The organic layer was washed with water, dried over Na2S0 , filtered, and concenfrated under reduced pressure to afford 7-ethoxy-5,8-dihydronaphthalen-l-ylamine (1.37 g, 76 % yield).
[Starting compound B]
8-amino-l,2,3,4-tetrahydro-naphthalen-2-ol
Figure imgf000101_0001
To a stined solution of 7-ethoxy-5,8-dihydronaphthalen-l-ylamine (1.07 g, 5.65 mmol) in tetrahydrofuran (30 mL) was added solution of aqueous 2N HCl (10 mL), and stiired at 40°C for 1 hour. The mixture was neutralized with addition of sodium bicorbonate, and the product was extracted with ethylacetate. The organic layer was washed with water, dried over Na2S04, filtered, and concentrated under reduced pressure to afford 8-amino-3,4-dihydro-lH-naphthalen-2- one (0.71 g, 78 % yield).
Next, to 8-amino-3,4-dihydro-lH-naphthalen-2-one (0.050 g, 0.318 mmol) in methanol (10 mL) was added sodium borohydride (0.030 g, 0.175 mmol) at 0°C, and the mixture was stined for 1 hour. The mixture was poured into water, and the product was exfracted with ethylacetate. The organic layer was dried over Na2S04, filtered, and concentrated under reduced pressure to afford i amino-l,2,3,4-tefrahydro-naphthalen-2-ol (0.037 g, 71 % yield).
[Starting compound C]
8-Amino-l ,2,3,4-tetrahy dro-naphthaIen-2-ol (enantiomer)
Figure imgf000102_0001
To a stined solution of benzenerathenium(H) chloride dimer (3.10 mg, 0.006 mmol) and (IS, 2R)- (-)-cis-l-amino-2-indanol (3.7 mg, 0.025 mmol) in degaussed isopropanol was heated at 80°C for 20 minutes under argon. The mixture was added to the solution of 8-amino-3,4-dihydro-lH- naphthalen-2-one (50 mg, 0.310 mmol) in isopropanol (3 mL) at room temperatare. A solution of potassium hydroxide (3.48 mg, 0.062 mmol) in isopropanol (1 mL) was added, and the mixtare was stiired at 45°C for 1 hour. The mixture was passed through silica gel and washed with ethylacetate. The filtrate was concentrated under reduced pressure to afford 8-amino-l, 2,3,4- tetrahydro-naphthalen-2-ol enantiomer (33.0 mg, 65 % yield).
The other enantiomer of 8-amino-l, 2,3 ,4-tetrahydronaphthalen-2-ol was obtained in the same fashion replacing (lS,2R)-(-)-cis-l-amino-2-indanol with (lR,2S)-(+)-cis-l-amino-2-indanol.
[Example 1-1]
5-chloro-N-(7-hydroxy-5,6,7,8-tetrahydronaphthalen-l-yl)-lH-indole-2-carboxamide
Figure imgf000102_0002
To 8-amino-l,2,3,4-tefrahydronaphthalen-2-ol (25.0 mg, 0.15 mmol) in tetrahydrofuran (2 mL) was added 5-chloro-lH-indole-2-carboxylic acid (30.0 mg, 0.15 mmol), l,l'-carbonyldi( 1,2,4- triazole) (31.6 mg, 0.15 mmol), and pyridine (12.1 mg, 0.15 mmol) at room temperature. After the mixture was stined for 5 hours, water was added and then exfracted with ethylacetate. The organic layer was dried over MgS04, filtered, and concentrated under reduced pressure. The obtained residue was washed with diethylether to provide 5-chloro-N-(7-hydroxy-5,6,7,8-tefrahydro- naphthalen-l-yl)-lH-indole-2-carboxamide (10.3 mg). Molecular weight : 340.81
MS (EST) m/z 341 [M+H]+
Melting Point: 254.3
Activity Class: B
In the similar manner as described in Example 1-1, compounds in Example 1-2 to 1- 4 as shown in Table 1 were synthesized.
Tablel
Figure imgf000103_0001
[Starting compound D]
2-bromo-N-(7-hydroxy-5,6,7,8-tetrahydronaphthalen-l-yl)acetamide
Figure imgf000104_0001
To a mixtare of 8-amino-3,4-dihydronaphthalen-2(lH)-one (1.67 g, 20.2 mmol) and pyridine (0.949 g, 12.0 mmol) in tetrahydrofuran (80 mL) was added bromoacetyl chloride (1.73 g, 11.0 mmol) in tetrahydrofuran (20 mL) at 0 °C. After the mixture was stined for 2 hours at room temperature, water (50 mL) was added and extracted with ethylacetate. The organic layer was dried over MgS04, filtered, and concentrated under reduced pressure. The obtained residue was purified by silica gel column chromatography (eluent: ethylacetate / hexane = 1 / 2) to provide 2- bromo-N-(7-oxo-5,6,7,8-tetrahydronaphthalen-l-yl)acetamide (2.18 g).
Molecular weight : 282.14
MS (ESI) : m/z 283 [M+H]+
Η NMR (CDC -d) δ 2.48 (t, J= 6.0 Hz, 2H), 3.05 (t, J= 6.0 Hz, 2H), 3.47 (s, 2H), 4.30 (s, 2H), 7.14 - 7.28 (m, 3H), 9.76 (brs, IH).
To a solution of 2-bromo-N-(7-oxo-5,6,7,8-tetrahydronaphthalen-l-yl)acetamide (564 mg, 2.00 mmol) in methanol (10 mL) was added sodium borohydride at 0°C. After the mixture was stined for 30 minutes, water (2 mL) was added and then concentrated under reduced pressure. The resulting residue was mixed with tetrahydrofuran and filtered. The filfrate was concentrated under reduced pressure to afford 2-bromo-N-(7-hydroxy-5,6,7,8-tetrahydronaphthalen-l-yl)acetamide (558 mg).
Molecular weight : 284.15
MS (ESI) m z 285 [M+H]+ [Example 2-1]
2N-[4-cMoro-3-(trifluoromethyl)phenyl]-lN-(7-hydroxy-5,6,7,8-tetrahydronaphthalen-l- yl)glycinamide
Figure imgf000105_0001
A mixtare of 2-bromo-N-(7-hydroxy-5,6,7,8-tefrahydronaphthalen-l-yl)acetamide (141 mg, 0.50 mmol) and 4-chloro-3-trifluoromethylaniline (93.9 mg, 0.48 mmol) in dimethylsulfoxide (7 mL) was stined at room temperature for 16 hours. To the reaction mixture was added potassium carbonate (138 mg, 1.00 mmol) and stined at 50 °C for 48 hours. The mixture was poured into water and extracted with ethyl acetate. The organic layer was dried over MgS04, filtered, and concentrated under reduced pressure. The obtained residue was purified by silica gel column chromatography (eluent: ethyl acetate / hexane = 1 / 1) to give 2N-[4-chloro-3-(trifluoromethyl)- phenyl]-lN-(7-hydroxy-5,6,7,8-tetrahydronaphthalen-l-yl)glycinamide (28.1 mg).
Molecular weight : 398.82
MS (ESI) m/z 399 [M+H]+
HPLC Retention Time: 4.45 minutes (Method A)
Activity Class: A
[Starting compound E]
ethyl-N-methyl-N-[4-(trifluoromethoxy)phenyl]glycinate
Figure imgf000105_0002
N-Methyl-4-trifluoromethoxyaniline (100 mg, 0.52 mmol), ethyl bromoacetate (262 mg, 1.57 mmol) and sodium carbonate (166 mg, 1.57 mmol) are reacted in dimethylacetamide (5 ml) at 60 °C over night. The reaction mixtare is partitioned between ethyl acetate and water, the organic layer is dried over magnesium sulfate and evaporated to dryness in vacuo. The raw material is purified by preparative HPLC with an acetonitrile/water gradient.
Yield: 106 mg (73 %)
Η NMR (400 MHz, DMSO-d6) δ 1.15 (t, 3H), 2.98 (s, 3H), 4.10 (q, 2H), 4.21 (s, ITT), 6.70 (d, ITT), 7.12 (d, 2H).
MS (EST): 278.1 [M+H]+
HPLC: Retention time 4.9 min (method B).
[Starting compound F]
N-methyl-N-[4-(frifluoromethoxy)phenyl]glycine
Figure imgf000106_0001
Ethyl-Ν-methyl-Ν-[4-(trifluoromethoxy)phenyl]glycinate (200 mg, 0.72 mmol) and potassium hydroxide (81 mg, 1.44 mmol) are dissolved in methanol/water (3 ml/1 ml) and stined for 1 h at room temperature. The reaction mixtare is acidified with 0.5 N hydrochloric acid to pH = 3 and partitioned between ethyl acetate and water. The organic extracts are dried over magnesium sulfate and evaporated to dryness in vacuo. The raw material is purified by preparative chromatography on silica (eluent: ethyl acetate/methanol, 1:0 - 5:1).
Yield: 35 mg (18 %)
Η NMR (300 MHz, DMSO-d6) δ 2.97 (s, 3H), 4.05 (s, 2H), 6.67 (d, 2H), 7.12 (d, 2H).
MS (EST): 247.9 [M-H]-
HPLC: Retention time 4.2 min (method B).
[Starting compound G]
2-bromo-N-[(7R)-7-hydroxy-5,6,7,8-tetrahydronaphthalen-l-yl]acetamide
Figure imgf000107_0001
(2R)-8-Amino-l,2,3,4-tefrahydro-naphthalen-2-ol (1.20 g, 7.35 mmol) is dissolved in ethyl acetate (38 ml). Saturated aqueous sodium hydrogencarbonate (19 ml) is added, the mixtare is stined vigorously and bromoacetyl chloride (1.16 g, 7.35 mmol) is added slowly. Stirring continues for 10 minutes, the aqueous layer is separated and the organic layer is dried over magnesium sulfate, filtered and evaporated to dryness. The raw material is triturated with diethyl ether, filtered and dried in vacuo.
Yield: 1.65 g (79 %)
Η NMR (200 MHz, DMSO-d6) δ 1.48-1.70 (m, IH), 1.78-1.95 (m, IH), 2.41 (dd, IH), 2.60-2.95 (m, 3H), 3.80-3.98 (m, IH), 4.08 (s, 2H), 4.84 (br s, IH), 6.93 (d, IH), 7.08 (t, IH), 7.18 (d, IH), 9.61 (s, IH).
MS (ESI*): 301 [M+NH4]+
HPLC: Retention time 3.40 min (method B).
[Starting compound H]
5-[4-(frifluoromethoxy)phenyl]pyridine-2-carboxylic acid
Figure imgf000107_0002
Under an argon atmosphere, to 4 ml 1,2-dimethoxyethane are added 5-brorno-pyridine-2- carboxylic acid (93 mg, 0.46 mmol), [4-(trifluoromethoxy)phenyl]boronic acid (114 mg, 0.55 mmol), 0.51 ml of a 2M aqueous sodium carbonate solution and dichlorobis- (triphenylphosphin)palladium(H) (20 mg, 0.03 mmol). The mixtare is stined at 90 °C overnight, cooled and quenched with water. Ethyl acetate is added and the mixture adjusted to pH = 2 with IN hydrochloric acid. After threefold extraction with ethyl acetate, the combined organic layers are dried over magnesium sulfate, filtered, and evaporated in vacuo. The residue is purified by silica gel chromatography (eluent: dichloromethane/methanol 5:1).
Yield: 56 mg (43 %).
MS (EST): 282 [M-H]'
HPLC: Retention time 4.01 min (method B)
[Starting compound I]
5-[4-(trifluoromethyl)phenyl]pyridine-2-carboxylic acid
Figure imgf000108_0001
The compound is obtained accordingly to the procedure for starting compound H from 5-bromo- pyridine-2-carboxylic acid (93 mg, 0.46 mmol) and [4-(frifluoromethyl)phenyl]boronic acid (105 mg, 0.55 mmol).
Yield: 76 mg (62 %).
LC-MS (ESI*): 268 [M+H]+; Retention time: 1.97 min (method E)
[Starting compound J]
methyl 6-[4-(frifluoromethoxy)phenyl]nicotinate
Figure imgf000108_0002
Under an argon atmosphere, to 4 ml 1,2-dimethoxyethane are added methyl 6-chloronicotinate (230 mg, 1.06 mmol), [4-(frifluoromethoxy)phenyl]boronic acid (268 mg, 1.31 mmol), 1.28 ml of a 2M aqueous sodium carbonate solution and tefralαs-(triphenylphosphin)palladium(0) (62 mg, 0.05 mmol). The mixtare is stined at 80 °C for 16h, cooled and quenched with water. After threefold exfraction with ethyl acetate, the combined organic layers are washed with brine, dried over magnesium sulfate, filtered, and evaporated in vacuo. The residue is purified by silica gel chromatography (eluent: cyclohexane/ethyl acetate 7:1).
Yield: 180 mg (57 %).
Η NMR (400 MHz, DMSO-d6) δ 3.92 (s, 3H), 7.53 (d, 2H), 8.17 (d, IH), 8.30 (d, 2H), 8.40 (dd, lH), 9.18 (d, IH).
MS (ESf): 298 [M+H]+
HPLC: Retention time 5.01 min (method B)
[Starting compound K]
methyl 6-[4-(trifluoromethyl)phenyl]nicotinate
Figure imgf000109_0001
The compound is obtained accordingly to the procedure for starting compound J from methyl 6- chloronicotinate (l.OOg, 5.83 mmol) and [4-(rrifluoromethyl)phenyl]boronic acid (1.33 g, 6.99 mmol).
Yield: 1.06 mg (65 %).
!H NMR (300 MHz, DMSO-d6) δ 3.92 (s, 3H), 7.90 (d, 2H), 8.25 (dd, IH), 8.38 (d, ITT), 8.42 (dd, IH), 9.21 (dd, IH).
MS (ESf): 282 [M+H]+
HPLC: Retention time 4.88 min (method B)
[Starting compound L]
6-[4-(frifluoromethoxy)phenyl]nicotinic acid
Figure imgf000110_0001
Methyl 6-[4-(trifluoromethoxy)phenyl]nicotinate (170 mg, 0.57 mmol) and powdered potassium hydroxide (96 mg, 1.72 mmol) are dissolved in 2 ml methanol and 0.05 ml water. After stirring the mixture at 40 °C overnight, the methanol is evaporated in vacuo. The residue is taken up with water and ethyl acetate and the aqueous phase is adjusted to pH=2 with IN hydrochloric acid. After threefold exfraction with ethyl acetate, the combined organic layers are washed with brine, dried over magnesium sulfate, and evaporated. The remaining residue is treated with diethyl ether, filtered, washed with diethyl ether and dried.
Yield: 148 mg (91 %).
Η NMR (400 MHz, DMSO-d6) δ 7.58 (d, 2H), 8.21 (d, IH), 8.36 (d, 2H), 8.42 (dd, IH), 9.22 (d, IH) 13.50 (s, IH).
MS (EST): 284 (M+H)+
HPLC: Retention time 4.33 min (method B)
[Starting compound M]
6-[4-(trifluoromethyl)phenyl]nicotinic acid
Figure imgf000110_0002
The compound is obtained accordingly to the procedure for starting compound L from methyl 6-[4- (trifluoromethyl)phenyl]nicotinate (250 mg, 0.89 mmol).
Yield: 212 mg (89 %). JH NMR (400 MHz, DMSO-d6) δ 7.90 (d, ITT), 8.23 (d, IH), 8.35-8.42 (m, 3H,), 9.19 (d, IH), 13.3-13.7 (broad s, IH).
MS (ESf): 268 [M+H]+
HPLC: Retention time 4.40 min (method B)
[Example 3-1]
N-[(7R)-7-hydroxy-5,6,7,8-tetrahydronaphthalen-l-yl]-N2-methyl-N2-[4-(trifluoromethoxy)- phenyl] glycinamide
Figure imgf000111_0001
(2R)-8-Amino-l,2,3,4-tefrahydro-naphthalen-2-ol (21 mg, 0.13 mmol), N'-(3-dimethylamino- propyl)-N-ethylcarbodiimide hydrochloride (32 mg, 0.17 mmol), 1 -hydroxy- IH-benzotriazole (21 mg, 0.15 mmol) and N-methyl-N-[4-(trifluoromethoxy)phenyl] glycine (35 mg, 0.14 mmol) are dissolved in dimethylacetamide (3 ml). The reaction mixture is stined over night at room temperature, partitioned between ethyl acetate and water, dried over magnesium sulfate and evaporated to dryness in vacuo. The raw material is purified by chromatography on silica (eluent: cyclohexane/ethyl acetate, 1:1).
Yield: 24 mg (45 %).
Η NMR (300 MHz, DMSO-d6) δ 1.5-1.65 (m, IH), 1.78-1.90 (m, IH), 2.40 (dd, IH), 2.62-2.90 (m, 3H), 3.32-3.44 (m, IH), 4.18 (s, ITT), 6.75 (d, 2H), 6.90 (d, IH), 7.04 (t, IH), 7.12-7.22 (m, 3H), 9.20 (s, IH).
MS (ESf): 395.0 [M+H]+
HPLC: Retention time 4.4 min (method B)
[Example 3-2]
N-[(7R)-7-hydroxy-5,6,7,8-tefrahydronaphthalen-l-yl]-N2-[4-(trifluoromethoxy)phenyl]- glycinamide ill
Figure imgf000112_0001
2-Bromo-N-[(7R)-7-hydroxy-5,6,7,8-tetrahydronaphthalen-l-yl]acetamide (100 mg, 0.35 mmol), 4- trifluoromethoxyaniline (62 mg, 0.35 mmol) and triethylamine (71 mg, 0.70 mmol) are dissolved in dry dimethylformamide (2 ml) and stined at 60 °C for 2 h. The mixtare is partitioned between ethyl acetate and water, the organic layer is dried over magnesium sulfate and evaporated to dryness. The raw material is purified by preparative chromatography on silica (eluent: cyclohexane/ethyl acetate, 2:1 - 0:1).
Yield: 8 mg (6 %).
Η NMR (200 MHz, DMSO-d6) δ 1.40-1.70 (m, IH), 1.73-1.92 (m, IH), 2.35 (dd, IH), 2.55-2.95 (m, 3H), 3.80-4.05 (m, 3H), 4.80 (d, IH), 6.38 (t, IH), 6.66 (d, 2H), 6.90 (d, IH), 7.00-7.20 (m, 3H), 7.27 (d, IH), 9.23 (s, IH).
MS (ESf): 381.3 [M+H]+
HPLC: Retention time 4.35 min (method B).
[Example 3-3]
N-[(7R)-7-hydroxy-5,6,7,8-tefrahydronaphthalen-l-yl]-4-(trifluoromethoxy)benzamide
Figure imgf000112_0002
(2R)-8-Amino-l,2,3,4-tefrahydro-naphthalen-2-ol (70 mg, 0.43 mmol), N'-(3-dimethylamino- propyl)-N-ethylcarbodiimide hydrochloride (107 mg, 0.56 mmol), 1-hydroxy-lH-benzotriazole hydrate (70 mg, 0.52 mmol) and 4-trifluoromethoxybenzoic acid (97 mg, 0.47 mmol) are dissolved in dimethylformamide (3 ml). The reaction mixture is stined over night at room temperature and then evaporated to dryness in vacuo. The raw material is solved in DMSO and purified by HPLC. Yield: 96 mg (64 %).
JH NMR (300 MHz, DMSO-d6) δ 1.73-1.83 (m, IH), 2.02-2.15 (m, IH), 2.92-3.19 (m, 3H), 3.98- 4.12 (m, ITT), 5.00 (d, IH), 7.19-7.33 (m, 3H), 7.72 (d, IH), 8.29 (d, 2H), 10.12 (s, IH).
LC-MS (ESf): 352.1 [M+H]+; Retention time: 2.88 min (method G)
[Example 3-4]
N-[(7R)-7-hydroxy-5,6,7,8-tefrahydronaphthalen-l-yl]-4-(trifluoromethyl)benzamide
Figure imgf000113_0001
(2R)-8-Amino-l,2,3,4-tetrahydro-naphthalen-2-ol (70 mg, 0.43 mmol), Ν'-(3-dimethylamino- propyl)-N-ethylcarbodiimide hydrochloride (107 mg, 0.56 mmol), 1 -hydroxy- IH-benzotriazole hydrate (70 mg, 0.52 mmol) and 4-trifluoromethylbenzoic acid (90 mg, 0.47 mmol) are dissolved in dimethylformamide (3 ml). The reaction mixtare is stined over night at room temperatare and then evaporated to dryness in vacuo. The raw material is solved in DMSO and purified by HPLC.
Yield: 110 mg (76 %).
Η NMR (300 MHz, DMSO-d6) δ 1.55-1.67 (m, IH), 1.87-1.92 (m, IH), 2.48 (dd, IH), 2.72-2.96 (m, 3H), 3.87-3.91 (m, IH), 4.75 (dd, IH), 7.15-7.01 (m, 3H), 7.91 (d, 2H), 8.16 (d, 2H), 10.00 (s, IH).
LC-MS (ESf ): 336.1 [M+H]+; Retention time: 2.84 min (method G)
[Example 3-5]
N-[(7R)-7-hydroxy-5,6,7,8-tefrahydronaphthalen-l-yl]-2-[4-(trifluoromethyl)phenylacetamide
Figure imgf000114_0001
(2R)-8-Amino-l,2,3,4-tetrahydro-naphthalen-2-ol (70 mg, 0.43 mmol), N'-(3-dimethylamino- propyl)-N-ethylcarbodiimide hydrochloride (107 mg, 0.56 mmol), 1-hydroxy-lH-benzotriazole hydrate (70 mg, 0.52 mmol) and [4-(trifluoromethyl)phenyl]acetic acid (96 mg, 0.47 mmol) are dissolved in dimethylformamide (3 ml). The reaction mixture is stined over night at room temperature and then evaporated to dryness in vacuo. The raw material is solved in DMSO and purified by HPLC.
Yield: 108 mg (72 %).
TI MR (300 MHz, DMSO-d6) δ 1.53-1.65 ( , IH), 1.83-1.88 (m, IH), 2.42 (dd, IH), 2.66-2.91 (m, 3H), 3.80 (s, 2H), 3.86-3.90 (m, IH), 4.77 (d, IH), 6.91 (d, IH), 7.04 (t, IH), 7.18 (d, IH), 7.57 (d, 2H), 7.70 (d, IH), 9.43 (s, IH).
LC-MS (ESf): 350.1 [M+H]+; Retention time: 2.86 min (method G)
[Example 3-6]
N-[(7R)-7-hydroxy-5,6,7,8-tefrahydronaphthalen-l-yl]-3-[4-(trifluoromethyl)phenyl]propanamide
Figure imgf000114_0002
(2R)-8-Amino-l,2,3,4-tetrahydro-naphthalen-2-ol (70 mg, 0.43 mmol), Ν'-(3-dimethylamino- propyl)-N-ethylcarbodiimide hydrochloride (107 mg, 0.56 mmol), 1-hydroxy-lH-benzotriazole hydrate (70 mg, 0.52 mmol) and [4-(trifluoromethyl)phenyl]propanoic acid (103 mg, 0.47 mmol) are dissolved in dimethylformamide (3 ml). The reaction mixture is stined over night at room temperatare and then evaporated to dryness in vacuo. The raw material is solved in DMSO and purified by HPLC. Yield: 85 mg (55 %).
Η NMR (300 MHz, DMSO-ds) δ 1.52-1.64 (m, IH), 1.82-1.87 (m, IH), 2.37 (dd, IH), 2.66-2.90 (m, 5H), 3.01 (t, 2H), 3.84 (m, IH), 4.74 (d, IH), 6.89 (d, IH), 7.03 (t, IH), 7.13 (d, IH), 7.50 (d, 2H), 7.65 (d, IH), 9.14 (s, IH).
LC-MS (ESf): 364.1 [M+H]+; Retention time: 2.97 min (method G)
[Example 3-7]
N-[(7R)-7-hydroxy-5,6,7,8-tefrahydronaphthalen-l-yl]-2-[4-(trifluoromethoxy)phenylacetamide
Figure imgf000115_0001
(2R)-8-Amino-l,2,3,4-tefrahydro-naphthalen-2-ol (100 mg, 0.61 mmol), N'-(3-dimethylamino- propyl)-N-ethylcarbodiimide hydrochloride (153 mg, 0.80 mmol), 1 -hydroxy- IH-benzotriazole hydrate (99 mg, 0.74 mmol) and [4-(trifluoromethoxy)phenyl]acetic acid (148 mg, 0.67 mmol) are dissolved in dimethylformamide (3 ml). The reaction mixtare is stined over night at room temperature and then evaporated to dryness in vacuo. The raw material is solved in DMSO and purified by HPLC.
Yield: 170 mg (76 %).
!H NMR (300 MHz, DMSO-d6) δ 1.51-1.65 (m, IH), 1.76-1.92 (m, IH), 2.41 (dd, IH), 2.79-2.87 (m, 3H), 3.72 (s, 2H), 3.81-3.94 (m, IH), 4.85 (d, IH), 6.91 (d, IH), 7.05 (t, IH), 7.17 (d, IH), 7.33 (d, 2H), 7.45 (d, IH), 9.44 (s, IH).
LC-MS (ESf): 366.0 [M+H]+; Retention time: 2.08 min (method F)
[Example 3-8]
2-(4-chlorophenoxy)-N-[(7R)-7-hydroxy-5,6,7,8-tefrahydronaphthalen-l-yl]acetamide
Figure imgf000116_0001
Under an argon atmosphere, (2R)-8-amino-l,2,3,4-tefrahydro-naphthalen-2-ol (150 mg, 0.92 mmol), N'-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (229 mg, 1.19 mmol), 1-hydroxy-lH-benzotriazole (149 mg, 1.10 mmol) and (4-chlorophenoxy)acetic acid (189 mg, 1.01 mmol) are added to 2 ml DMF at room temperature and the reaction is stined overnight. Water is then added and the resulting mixture is exfracted with ethyl acetate three times. The combined organic phases are washed with brine, dried over magnesium sulfate and evaporated in vacuo. The residue is purified first by chromatography on silica gel (eluent cyclohexane/ethyl acetate 2:1), then by preparative reversed phase HPLC (eluent water/acetonitrile gradient). After collecting the appropriate product fractions and evaporating the solvent in vacuo, the residue is washed thoroughly with diethyl ether and dried to give the target compound
Yield: 227 mg (74 %).
Η NMR (400 MHz, DMSO-d6) δ 1.53-1.65 (m, IH), 1.81-1.91 (m, IH), 2.42 (dd, IH), 2.73 (ddd, IH), 2.84 (dd, IH), 2.88 (dd, IH), 3.84-3.94 (m, IH), 4.72 (s, 2H), 4.81 (d, IH), 6.95 (d, IH), 7.05 (d, 2H), 7.09 (d, IH), 7.21 (d, IH), 7.37 (d, 2H), 9.39 s, IH).
MS (ESf): 332 [M+H]+
HPLC: Retention time 4.23 min (method B)
[Example 3-9]
2-(2,4-difluorophenoxy)-N-[(7R)-7-hydroxy-5,6,7,8-tetrahydronaphthalen-l-yl]acetamide
Figure imgf000116_0002
The compound is obtained accordingly to the procedure for Example 3-8 from (2R)-8-amino- l,2,3,4-tetrahydro-naphthalen-2-ol (150 mg, 0.92 mmol) and (2,4-difluorophenoxy)acetic acid (190 mg, 1.01 mmol).
Yield: 199 mg (65 %)
TJ NMR (400 MHz, DMSO-d6) δ 1.53-1.66 (m, IH), 1.81-1.92 (m, IH), 2.42 (dd, IH), 2.73 (ddd, IH), 2.84 (dd, IH), 2.88 (dd, IH), 3.84-3.94 (m, IH), 4.80 (s, 2H), 4.81 (d, IH), 6.94 (d, IH), 7.00- 7.12 (m, 2H), 7.20 (dt, IH), 7.26 (d, IH), 7.33 (ddd, IH) 9.35 (s, IH)..
MS (ESf): 334 [M+H]+
HPLC: Retention time 4.11 min (method B)
[Example 3-10]
2-[2-chloro-4-(trifluoromethyl)phenoxy]-Ν-[(7R)-7-hydroxy-5,6,7,8-tefrahydronaphthalen-l- yl]acetamide
Figure imgf000117_0001
The compound is obtained accordingly to the procedure for Example 3-8 from (2R)-8-amino- l,2,3,4-tefrahydro-naphthalen-2-ol (80 mg, 0.49 mmol) and [2-chloro-4-(trifluoromethyl)- phenoxy] acetic acid (137 mg, 0.54 mmol).
Yield: 150 mg (77 %).
Η NMR (xOO MHz, DMSO-d6) δ 1.55-1.67 (m, IH), 1.82-1.92 (m, IH), 2.46 (dd, IH), 2.73 (ddd, IH), 2.86 (dd, IH), 2.90 (dd, IH), 3.86-3.96 (m, IH), 4.84 (d, IH), 4.50 (s, 2H), 6.94 (d, IH), 7.09 (t, IH), 7.31(d, IH), 7.35 (d, IH), 7.72 (dd, IH), 7.89 (d, IH), 9.34 (s, IH).
MS (ESf): 400 [M+H]+
HPLC: Retention time 4.64 min (method D) [Example 3-11]
N-[(7R)-7-hydroxy-5,6,7,8-tefrahydronaphthalen-l-yl]-2-[4-(trifluoromethyl)phenoxy]-acetamide
Figure imgf000118_0001
The compound is obtained accordingly to the procedure for Example 3-8 (omitting the first chromatography over silica gel) from (2R)-8-amino-l,2,3,4-tetrahydro-naphthalen-2-ol (100 mg, 0.61 mmol) and [4-(trifluoromethyl)phenoxy]acetic acid (148 mg, 0.67 mmol).
Yield: 153 mg (68 %).
!H NMR (400 MHz, DMSO-d6) δ 1.54-1.66 (m, IH), 1.81-1.92 (m, IH), 2.43 (dd, IH), 2.73 (ddd, IH), 2.81-2.92 (m, 2H), 3.84-3.94 (m, IH), 4.81 (d, IH), 4.83 (s, 2H), 6.96 (d, IH), 7.08 (t, IH), 7.16-7.24 (m, 2H), 7.70 (d, IH), 9.46 (s, IH).
MS (Cf ): 383 [M+NH4]+
HPLC: Retention time 4.38 min (method B)
[Example 3-12]
N-[(7R)-7-hydroxy-5,6,7,8-tefrahydronaphthalen-l-yl]-2-[4-(trifluoromethoxy)phenoxy]-acetamide
Figure imgf000118_0002
The compoimd is obtained accordingly to the procedure for Example 3-8 (the crude reaction mixture is applied to reversed phase HPLC purification directly) from (2R)-8-amino-l,2,3,4- tefrahydro-naphthalen-2-ol (80 mg, 0.49 mmol) and [4-(trifluoromethoxy)phenoxy]acetic acid (127 mg, 0.54 mmol).
Yield: 119 mg (64 %). , Η NMR (400 MHz, DMSO-d6) δ 1.54-1.66 (m, IH), 1.81-1.91 (m, IH), 2.42 (dd, IH), 2.73 (ddd, IH), 2.84 (dd, IH), 2.88 (dd, IH), 3.84-3.94 (m, IH), 4.75 (s, 2H), .81 (d, IH), 6.96 (d, IH), 7.09 (t, IH), 7.12 (d, IH), 7.21 (d, IH), 7.34 (d, IH), 9.41 (s, IH).
MS (Cf ): 399 [M+NH4]+
HPLC: Retention time 4.44 min (method B)
[Example 3-13]
N-[(7R)-7-hydroxy-5,6,7,8-tetrahydronaphthalen-l-yl]-5-[4-(trifluoromethoxy)phenyl]-pyridine-2- carboxamide
Figure imgf000119_0001
The compound is obtained accordingly to the procedure for Example 3-8 (the crude reaction mixture is applied to reversed phase HPLC purification directly) from (2R)-8-amino-l, 2,3,4- tefrahydro-naphthalen-2-ol (25 mg, 0.15 mmol) and 5-[4-(trifluoromethoxy)phenyl]pyridine-2- carboxylic acid (50 mg, 0.18 mmol).
Yield: 20 mg (31 %).
Η NMR (400 MHz, DMSO-d6) δ 1.41-1.52 (m, IH), 1.68-1.76 (m, IH), 2.37 (dd, IH), 2.59 (dd, IH), 2.74 (dt, IH), 2.81 (dd, IH), 3.75-3.85 (m, IH), 4.71 (d, IH), 6.79 (d, IH), 6.99 (t, IH), 7.38 (d, ITT), 7.53-7.61 (m, IH), 7.82 (d, 2H), 8.07 (d, IH), 8.22 (dd, IH), 8.91 (d, IH), 10.01 (s, IH).
LC-MS (ESf): 429 [M+H]+; Retention time: 2.81 min (method E)
[Example 3-14]
N-[(7R)-7-hydroxy-5,6,7,8-tetrahydronaphthalen-l-yl]-5-[4-(trifluoromethyl)phenyl]-pyridine-2- carboxamide
Figure imgf000120_0001
The compound is obtained accordingly to the procedure for Example 3-8 (the crude reaction mixtare is applied to reversed phase HPLC purification directly) from (2R)-8-amino-l, 2,3,4- tefrahydro-naphthalen-2-ol (42 mg, 0.26 mmol) and 5-[4-(trifluoromethyl)phenyl]pyridine-2- carboxylic acid (75 mg, 0.28 mmol).
Yield: 29 mg (27 %).
Η NMR (300 MHz, DMSO-d6) δ 1.57-1.72 (m, IH), 1.85-1.96 (m, IH), 2.56 (dd, IH), 2.77 (ddd, IH), 2.91 (dt, IH), 2.99 (dd, IH), 3.92-4.04 (m, IH), 4.86 (d, IH), 6.97 (d, IH), 7.16 (t, IH), 7.74 (d, IH), 7.91 (d, 2H), 8.09 (d, IH), 8.27 (d, IH), 8.45 (dd, IH), 9.13 (d, IH), 10.19 (s, IH).
MS (ESf): 413 [M+H]+
HPLC: Retention time 4.88 min (method D)
[Example 3-15]
N-[(7R)-7-hydroxy-5,6,7,8-tefrahydronaphthalen-l-yl]-6-[4-(rrifluoromethyl)phenyl]-nicotinamide
Figure imgf000120_0002
The compound is obtained accordingly to the procedure for Example 3-8 (the crade reaction mixture is applied to reversed phase HPLC purification directly) from (2R)-8-amino-l,2,3,4- tefrahydro-naphthalen-2-ol (117 mg, 0.71 mmol) and 6-[4-(trifluoromethyl)phenyl]nicotinic acid (210 mg, 0.79 mmol).
Yield: 230 mg (78 %). Η NMR (400 MHz, DMSO-d6) δ 1.46-1.57 (m, IH), 1.75-1.84 (m, IH), 2.36-2.56 (dd., IH), 2.68 (ddd, IH), 2.76-2.88 (m, 2H), 3.75-3.85 (m, IH), 4.69 (d, IH), 6.93 (d, IH), 7.01-7.11 (m, 2H), 7.80 (d, 2H), 8.17 (d, IH), 8.30 (d, 2H), 8.36 (dd, IH), 9.16 (s, IH), 9.95 (s, IH).
MS (ESf): 413 [M+H]+
HPLC: Retention time 4.42 min (method B)
[Example 3-16]
N-[(7R)-7-hydroxy-5,6,7,8-tetrahydronaphthalen-l-yl]-6-[4-(trifluoromethoxy)phenyl]- nicotinamide
Figure imgf000121_0001
The compound is obtained accordingly to the procedure for Example 3-8 (the crade reaction mixture is applied to reversed phase HPLC purification directly) from (2R)-8-amino-l,2,3,4- tefrahydro-naphthalen-2-ol (37 mg, 0.23 mmol) and 6-[4-(trifluoromethoxy)phenyl]nicotinic acid (71 mg, 0.25 mmol).
Yield: 67 mg (69 %).
Η NMR (400 MHz, DMSO-d6) δ 1.63-1.75 (m, IH), 1.92-2.01 (m, IH), 2.54-2.63 (dd, IH), 2.85 (ddd, IH), 2.94-3.04 (m, 2H), 3.92-4.01 (m, IH), 4.86 (d, IH), 7.11 (d, IH), 7.18-7.28 (m, 2H), 7.60 (d, 2H), 8.27 (d, IH), 8.39 (d, 2H), 8.49 (dd, IH), 9.30(s, IH), 10.10 (s, IH).
MS (ESf): 429 [M+H]+
HPLC: Retention time 4.40 min (method B) CHAPTER IV (EXAMPLES')
Preparing method of compounds
[Starting compound A]
Figure imgf000122_0001
A mixtare of l,4-dioxaspiro[4.5]decan-8-one (3.12 g, 20.0 mmol), hydroxylamine hydrochloride (1.67 g, 24.0 mmol), and triethylamine (2.42 g, 24.0 mmol) in methanol (50 mL) was stined under reflux for 2 hours. The resulting mixtare was concentrated under reduced pressure and then purified by silica gel column chromatography (eluent: ethylacetate / hexane = 1 / 1) to provide 1,4- dioxaspiro[4.5]decan-8-one oxime (2.73 g).
Molecular weight : 171.20
MS (ESI) m/z 172 [M+H]+
*H NMR (COC -d) δ 1.26 (t, J= 7.2 Hz, 2H), 1.76 (t, J= 7.2 Hz, 2H), 2.41 (t, J= 6.5 Hz, 2H), 2.68 (t, J= 6.5 Hz, 2H), 3.99 (s, 4H), 7.80 (brs, IH).
Next, to a mixture of l,4-dioxaspiro[4.5]decan-8-one oxime (2.73 g, 16.0 mmol), allyl bromide (5.79 g, 47.8 mmol), and potassium carbonate (4.41 g, 31.9 mmol) in acetone (100 mL) was stined under reflux for 15 hours. After the mixture was cooled to ambient temperature, it was filtered and the filtrate was concentrated under reduced pressure. The obtained residue was purified by silica gel column chromatography (eluent: ethylacetate / hexane = 1 / 4) to give 1,4-dioxaspiro- [4.5]decan-8-one O-allyloxime (1.01 mg).
Molecular weight : 111.16 MS (ESI) m/z 212 [M+H]+
!H NMR (CDC -d) δ 1.76 (t, J= 7.0 Hz, ITT), 1.82 (t, J= 7.0 Hz, ITT), 2.40 (t, J= 6.5 Hz, 2H), 2.66 (t, J = 6.5 Hz, ITT), 3.98 (s, 4H), 4.53 (dd, J = 1.3, 4.3 Hz, 2H), 5.20 (dd, J = 1.3, 10.4 Hz, IH), 5.30 (d, J = 10.4 Hz, IH), 5.96 - 6.02 (m, IH).
Next, l,4-dioxaspiro[4.5]decan-8-one O-allyloxime (1.00 mg, 4.78 mmol) was heated neat at 230°C for 21 hours. After the residue was cooled to ambient temperatare, it was purified by silica gel column chromatography (eluent: tetrahydrofuran / hexane = 1 / 2) to afford 7',8'-dihydro-5'H- spiro[l,3-dioxolane-2,6'-quinoline] (105 mg).
Molecular weight : 191.23
MS (ESI) m/z 192 [M+H] ,+
]H NMR (CDC -d) δ 2.05 (t, J= 6.9 Hz, 2H), 3.15 (t, J= 6.9 Hz, 2H), 3.00 (s, 2H), 4.06 (s, 4H), 7.05 (dd, J= 4.8, 7.7 Hz, IH), 7.34 (d, J= 7.7 Hz, IH), 8.39 (d, J= 4.8 Hz, IH).
Next, 7',8l-dihydro-5'H-spiro[l,3-dioxolane-2,6l-quinoline] is freated with a mixtare of nitric acid and sulfuric acid and then the mixtare is heated to reflux. After cooled to room temperatare, water is added and the mixture is exfracted with ethyl acetate. Concenfration of the organic layer under reduced pressure yields 4-nitro-7,8-dihydroquinolin-6(5H)-one 1-oxide.
Next, a solution of 4-nitro-7,8-dihydroquinolin-6(5H)-one 1-oxide in tetrahydrofuran is freated under hydrogen atmosphere in the presence of catalytic amount of Pt/C. The mixtare is passed through celite and is concenfrated under reduced pressure to give 4-amino-7,8-dihydroquinolin- 6(5H)-one.
A solution of 4-amino-7,8-dihydroquinolin-6(5H)-one in tetrahydrofuran is freated with sodium borohydride. After stining for 6 hours, water is added. The mixture is extracted with ethyl acetate, dried and the organic layer is then concentrated under reduced pressure. The resulting residue is purified by silica gel column chromatography to give 4-amino-5,6,7,8-tefrahydroquinolin-6-ol. [Example 1-1]
N-[4-chloro-3-(trifluoromethyl)phenyl]-N'-(6-hydroxy-5,6,7,8-tetrahydroquinoIin-4-yl)urea
Figure imgf000124_0001
A mixture of 4-amino-5,6,7,8-tetrahydroquinolin-6-ol and 4-chloro-3-trifluoromethylphenyl iso- cyanate in tetrahydrofuran is stined at 50°C for 5 hours. After removing the solvent, the resulting residue is purified by silica gel column chromatography to provide N-[4-chloro-3-(trifluoro- methyl)phenyl]-Nl-(6-hydroxy-5,6,7,8-tefrahydroquinolin-4-yl)urea.
Li a similar manner as described in Example 1-1, Example 1-2 to 1- 8 as shown in Table 1 are synthesized.
Also, Example 2-1 to 2-8 as shown in Table 2, Example 3-1 to 3-8 as shown in Table 3, and Example 4-1 to 4-8 as shown in Table 4 are synthesized in a similar maimer as as described in Example 1-1.
Figure imgf000125_0001
Figure imgf000125_0002
Figure imgf000126_0001
Figure imgf000126_0002
Figure imgf000127_0001
Figure imgf000127_0002
Figure imgf000128_0001
Figure imgf000128_0002

Claims

Claims
1. A compound of the formula (A), their tautomeric and stereoisomeric form, and salts thereof:
Figure imgf000129_0001
wherein represents the formula
Figure imgf000129_0002
wherein
# represents the connection position to the molecule,
Qi and Q4 (Chapter I) independently represent direct bond or methylene;
Chemical bond between Op — Q3 (Chapter I) is selected from the group consisting of a single bond and a double bond; when Op — Q3 (Chapter I) is a single bond, Q2 (Chapter I) represents CHR2, or CO, and Q3 (Chapter I) represents CHR3, when Q, — Qp (Chapter I) is a double bond, Q2 (Chapter I) represents CR2 and Q3 (Chapter I) represents CR3; wherein
R2 (Chapter I) represents hydrogen, hydroxy, Cι-6 alkoxy or Cι-6 alkanoyloxy;
R3 (Chapter !) represents hydrogen, hydroxy, Cι-6 alkoxy, Cχ.6 alkanoyloxy, or Cι-6 alkyl optionally substitated by hydroxy, Cι-6 alkoxy or Cι-6 alkanoyloxy, with the proviso that Qi and Q (Chapter I) can not be direct bond at the same time;
R2 and R3 (Chapter I) can not be hydrogen at the same time; when Qi and Q4 (Chapter I) are both methylene and R3 (Chapter I) is hydroxy, R2 (Chapter I) is hydroxy, Cι-6 alkoxy or Cι-6 alkanoyloxy; when Qi (Chapter I) is direct bond, R2 (Chapter I) is hydroxy, Cι-6 alkoxy or Ci-e alkanoyloxy; and when Q (Chapter I) is direct bond, R2 (Chapter I) is hydrogen, Cι-6 alkoxy or Cι_6 alkanoyloxy;
Qi, Q2 and Q3 (Chapter TV) independently represent N or CH, with the proviso that at least one of Q Q2 and Q3 (Chapter IV) is N;
and represents the formula
# # If rr r FT or ^N T R' or
^ ' or ^A H A xX L k Jp R wherein # represents the connection position to the molecule n represents an integer of 0 to 6;
R4 represents aryl optionally having one or two substituents selected from the group consisting of halogen, hydroxy, Cι-6 alkylamino, di(Cι-6 alkyl)amino, C3-8 cycloalkylamino, C_-6 alkoxycarbonyl, phenyl, benzyl, sulfonamide, Cι.6 alkanoyl, Cι-6 alkanoylamino, carbamoyl, Cι-6 alkylcarbamoyl, cyano, Ci_6 allcyl optionally substitated by cyano, Cι-6 alkoxycarbonyl, or mono-, di-, or tri-halogen, Cι-6 alkoxy optionally substitated by mono-, di-, or tri- halogen, phenoxy optionally substitated by halogen or Cι_6 alkyl, and Cι-6 alkylthio optionally substitated by mono-, di-, or tri- halogen, R1 (Chapter H) represents C3-8cycloalkyl optionally fused by aryl, wherein said aryl is optionally substitated with one or more substitaents selected from the group consisting of halogen, hydroxy, carboxy, nifro, cyano, amino, Cι_6 alkylamino, di(Cι-6 alkyl)amino, Cι-6 alkoxycarbonyl, Cι-6 alkanoyl, Cι-6 alkanoylamino, carbamoyl, Cι-6 alkylcarbamoyl, -β alkyl optionally substitated by cyano, .6 alkoxycarbonyl, or mono-, di-, or tri-halogen, Cι-6 alkoxy optionally substitated by mono-, di-, or tri- halogen and Cι.6 alkylthio optionally substitated by mono-, di-, or tri- halogen; phenyl substitated by heteroaryl, or heteroaryloxy, wherein said heteroaryl and heteroaryloxy are optionally substitated with one or more substituents selected from the group consisting of halogen, hydroxy, carboxy, nitro, cyano, amino, Cι_6 alkylamino, di(Cι.6 alkyl)amino, Cι.6 alkoxycarbonyl, C__6 alkanoyl, Cι-6 alkanoylamino, carbamoyl, Cι-6 alkylcarbamoyl, Cι_6 alkyl optionally substitated by cyano, Cι_6 alkoxycarbonyl, or mono-, di-, or tri-halogen, C^ alkoxy optionally substituted by mono-, di-, or tri- halogen, and Q.6 alkylthio optionally substitated by mono-, di-, or tri- halogen; phenyl fused with heteroaryl, or heterocyclyl, wherein said heteroaryl is optionally substituted with one or more substitaents selected from the group consisting of halogen, hydroxy, carboxy, nitro, cyano, amino, Cι-6 alkylamino, di(Cι.6 alkyl)amino, .6 alkoxycarbonyl, Cι-6 alkanoyl, Cι.6 alkanoylamino, carbamoyl, Cι_6 alkylcarbamoyl, Cι-6 alkyl optionally substitated by cyano, Cι-6 alkoxycarbonyl, or mono-, di- , or tri-halogen, Cι-6 alkoxy optionally substitated by mono-, di-, or tri- halogen and -β alkylthio optionally substituted by mono-, di-, or tri- halogen; or heteroaryl optionally substitated with one or more substituents selected from the group consisting of halogen, hydroxy, carboxy, nitro, cyano, amino, phenyl, benzyl, Cι-6 alkylamino, di(Cι.6 alkyl)amino, Cι-6 alkoxycarbonyl, Cι-6 alkanoyl, Cι-6 alkanoylamino, carbamoyl, Cι.6 alkylcarbamoyl, Cι-6 alkyl optionally substitated by cyano, Cι.6 alkoxycarbonyl, or mono-, di-, or tri-halogen, Cι-6 alkoxy optionally substitated by mono-, di-, or tri- halogen and Cι-6 alkylthio optionally substitated by mono-, di-, or tri- halogen,
R1 (Chapter ID) represents aryl or heteroaryl, wherein said aryl and heteroaryl are optionally substituted with one or more substitaents selected from the group consisting of halogen, nifro, hydroxy, carboxy, amino, Cι-6 alkylamino, di(Cι_6 alkyl)amino, C3-8 cycloalkylamino, Ci_6 alkoxycarbonyl, phenyl (which phenyl is optionally substitated by halogen, xrifluoromethyl, xrifluoromethoxy, nitro, hydroxy, carboxy, amino, Cι_6 alkylamino, di(Cι-6 alkyl)amino, C3_8 cycloalkylamino, or Cι_6 alkoxycarbonyl), benzyl (in which phenyl moiety is optionally substitated by halogen, nitro, hydroxy, carboxy, amino, Cι_6 alkylamino, di(Cι-6 alkyl), amino, C3-8 cycloalkylamino, or C]-6 alkoxycarbonyl), heterocycle, sulfonamide, .6 alkanoyl, Cι.e alkanoylamino, carbamoyl, Cι_6 alkylcarbamoyl, cyano, Cι_6 alkyl (which alkyl is optionally substitated by cyano, nifro, hydroxy, carboxy, amino, Cι.6 alkoxycarbonyl or mono-, di-, or tri-halogen), C].6 alkoxy (which alkoxy is optionally substituted by mono-, di-, or tri- halogen), phenoxy (in which phenyl moiety is optionally substitated by halogen, nitro, hydroxy, carboxy, amino, Cι.6 alkylamino, di(Ci_6 alkyl)amino, C3.8 cycloalkylamino, or -β alkoxycarbonyl or -β alkyl), Cι.6 alkylthio (which alkylthio is optionally substituted by mono-, di-, or tri- halogen), C3.8 cycloalkyl, and heterocycle; Ci-e alkyl optionally substitated by R11, OR12, SR12 or N(R12)(R13), wherein
R1 ' represents aryl or heteroaryl, wherein said aryl and heteroaryl are optionally substitated with one or more substituents selected from the group consisting of halogen, nifro, hydroxy, carboxy, amino, C].6 alkylamino, di(Cι.6 alkyl)amino, C3.8 5 cycloalkylamino, C].6 alkoxycarbonyl, phenyl (which phenyl is optionally substitated by halogen, nifro, hydroxy, carboxy, amino, Cι.6 alkylamino, di(C].6 alkyl)amino, C3.8 cycloalkylamino, or Cι-6 alkoxycarbonyl), benzyl (in which phenyl moiety is optionally substitated by halogen, nitro, hydroxy, carboxy, amino, C .e alkyl-
10 amino, di(Cι.6 alkyl), amino, C3.8 cycloalkylamino, or Cι-6 alkoxycarbonyl), heterocycle, sulfonamide, Cι_6 alkanoyl, Cι.6 alkanoylamino, carbamoyl, Cι.6 alkylcarbamoyl, cyano, -β alkyl (which alkyl is optionally substitated by cyano, nitro, hydroxy, carboxy, amino, Cι_6 alkoxycarbonyl or mono-, di-, or tri-halogen),
15 Cι-6 alkoxy (which alkoxy is optionally substituted by mono-, di-, or tri- halogen), phenoxy (in which phenyl moiety is optionally substitated by halogen, nitro, hydroxy, carboxy, amino, Cι.β alkylamino, di(Cι_6 alkyl)amino, C3_8 cycloalkylamino, or C__6 alkoxycarbonyl or C].6 alkyl), Cι.6 alkylthio (which alkylthio is
20 optionally substitated by mono-, di-, or tri- halogen), C3.8 cycloalkyl, and heterocycle;
R12 represents aryl, heteroaryl, or Cι-6 alkyl optionally substituted by aryl or heteroaryl, wherein
25 said aryl and heteroaryl are optionally substituted with one or more substituents selected from the group consisting of halogen, nifro, hydroxy, carboxy, amino, Cι_6 alkylamino, di( .6 alkyl)amino, C3.8 cycloalkylamino, Cι_6 alkoxycarbonyl, phenyl (which phenyl is optionally substitated by halogen, nitro, hydroxy, carboxy, amino,
30 Ci.6 alkylamino, di(Cι_6 alkyl)amino, C3.8 cycloalkylamino, or Cι_6 alkoxycarbonyl), benzyl (in which phenyl moiety is optionally substitated by halogen, nitro, hydroxy, carboxy, amino, Cι.6 alkylamino, di(Ci.6 alkyl), amino, C3.8 cycloalkylamino, or -β alkoxycarbonyl), heterocycle, sulfonamide, Cι_6 alkanoyl, Cι.6 alkanoylamino, carbamoyl, .6 alkylcarbamoyl, cyano, Cι.6 alkyl (which allcyl is optionally substituted by cyano, nifro, hydroxy, carboxy, amino, Cι-6 alkoxycarbonyl or mono-, di-, or tri-halogen), Ci_6 alkoxy (which alkoxy is optionally substitated by mono-, di-, 5 or tri- halogen), phenoxy (in which phenyl moiety is optionally substituted by halogen, nifro, hydroxy, carboxy, amino, Cι_6 alkylamino, di(Q.6 alkyl)amino, C3.8 cycloalkylamino, or Cι.6 alkoxycarbonyl or Q.6 alkyl), .6 alkylthio (which alkylthio is optionally substitated by mono-, di-, or tri- halogen), C3.8 10 cycloalkyl, and heterocycle; and
R13 represents hydrogen, or Q.6 allcyl; or
Q-8cycloalkyl optionally fused by aryl, wherein
15 said aryl is optionally substitated with one or more substitaents selected from the group consisting of halogen, nifro, hydroxy, carboxy, amino, Q_6 alkylamino, di(Q.6 alkyl)amino, C3.8 cycloalkylamino, Q.6 alkoxycarbonyl, phenyl (which phenyl is optionally substitated by halogen, nifro, hydroxy, carboxy, amino, Q.6 alkylamino, di(Q_6 alkyl)amino, C3.8 cycloalkylamino,
20 or Ci-e alkoxycarbonyl), benzyl (in which phenyl moiety is optionally substitated by halogen, nifro, hydroxy, carboxy, amino, Q_6 alkylamino, di(Q_6 alkyl), amino, C3.8 cycloalkylamino, or .6 alkoxycarbonyl), heterocycle, sulfonamide, Q_6 alkanoyl, Q_6 alkanoylamino, carbamoyl, Q.6 alkylcarbamoyl, cyano, Q.6 allcyl (which alkyl is optionally substitated
25 by cyano, nifro, hydroxy, carboxy, amino, Q.6 alkoxycarbonyl or mono-, di-, or tri-halogen), Q.6 alkoxy (which alkoxy is optionally substituted by mono-, di-, or tri- halogen), phenoxy (in which phenyl moiety is optionally substitated by halogen, nifro, hydroxy, carboxy, amino, Q.6 alkylamino, di(Q.6 alkyl)amino, C3.8 cycloalkylamino, or Q.6 alkoxycarbonyl or Q_6
30 allcyl), Q.6 alkylthio (which alkylthio is optionally substitated by mono-, di-, or tri- halogen), Q_8 cycloalkyl, and heterocycle, m represents 0, 1, 2, or 3; p represents 0 or 1;
-X- represents a bond, -O- or -N(R!)- (wherein R1 is hydrogen or Q_6 allcyl); with the proviso that when m is 0, -X- represents a bond,
R represents aryl or heteroaryl, wherein said aryl and heteroaryl are optionally substitated with one or more substitaents independently selected from the group consisting of halogen, nifro, hydroxy, carboxy, amino, Q.6 alkylamino, di(Q_6 alkyl)amino, C3.8 cycloalkylamino, Q_β alkoxycarbonyl, phenyl (which phenyl is optionally substitated by halogen, nifro, hydroxy, carboxy, amino, Q.6 alkylamino, di(Q.6 alkyl)amino, C3.8 cycloalkylamino, or Q.6 alkoxycarbonyl), benzyl (in which phenyl moiety is optionally substitated by halogen, nitro, hydroxy, carboxy, amino, Q_6 alkylamino, di(Q_6 alkyl)amino, C3.8 cycloalkylamino, or Q_6 alkoxycarbonyl), sulfonamide, Q_6 alkanoyl, Q.6 alkanoylamino, carbamoyl, Q.6 alkylcarbamoyl, cyano, Q_6 allcyl (which alkyl is optionally substitated by cyano, nifro, hydroxy, carboxy, amino, Q.6 alkoxycarbonyl or mono-, di-, or tri-halogen), Q_6 alkoxy (which alkoxy is optionally substitated by mono-, di-, or tri- halogen), phenoxy (in which phenyl moiety is optionally substituted by halogen, nitro, hydroxy, carboxy, amino, Q-6 alkylamino, di(Q.6 alkyl)amino, C3.8 cycloalkylamino, or Q.6 alkoxycarbonyl or Q.6 allcyl), Q_6 alkylthio (which alkylthio is optionally substitated by mono-, di-, or tri- halogen), C3.8 cycloalkyl, and heterocycle.
2. Compound of formula (A) according to claim 1, with the formula (I), their tautomeric and stereoisomeric form, and salts thereof:
Figure imgf000135_0001
wherein n represents an integer of 0 to 6; Qi and Q independently represent direct bond or methylene;
Chemical bond between Q2__r__:Q3 is selected from the group consisting of a single bond and a double bond;
when Q? — Q3 is a single bond, Q2 represents CHR2, or CO, and Q3 represents CHR3,
when O? — Q3 is a double bond, Q2 represents CR2 and Q3 represents CR3; wherein
R2 represents hydrogen, hydroxy, Q_6 alkoxy or Q.6 alkanoyloxy;
R3 represents hydrogen, hydroxy, Q.6 alkoxy, Cι.6 alkanoyloxy, or Q.6 alkyl optionally substituted by hydroxy, Q.6 alkoxy or Q.6 alkanoyloxy, with the proviso that Qi and Q can not be direct bond at the same time;
R2 and R3 can not be hydrogen at the same time; when Qi and Q are both methylene and R3 is hydroxy, R2 is hydroxy, Q_6 alkoxy or Q_6 alkanoyloxy; when Qi is direct bond, R2 is hydroxy, Q_6 alkoxy or Q_6 alkanoyloxy; and when Q4 is direct bond, R2 is hydrogen, Q_6 alkoxy or Q.6 alkanoyloxy;
and
R4 represents aryl optionally having one or two substitaents selected from the group consisting of halogen, hydroxy, Q_6 allcylamino, di(Q.6 alkyl)amino, C3.8 cycloalkylamino, Q.6 alkoxycarbonyl, phenyl, benzyl, sulfonamide, Q.6 alkanoyl, Cι-6 alkanoylamino, carbamoyl, Q.6 alkylcarbamoyl, cyano, Q.6 allcyl optionally substitated by cyano, Q.6 alkoxycarbonyl, or mono-, di-, or tri-halogen, Q.6 alkoxy optionally substitated by mono-, di-, or tri- halogen, phenoxy optionally substitated by halogen or Q.6 alkyl, and Q_6 alkylthio optionally substitated by mono-, di-, or tri- halogen.
Compoimd of formula (A) according to claim 1, with the formula (I), their tautomeric and stereoisomeric form, and salts thereof:
Figure imgf000137_0001
wherein
n represents an integer of 0 to 6; and
R1 represents Q.8cycloalkyl optionally fused by aryl, wherein said aryl is optionally substitated with one or more substituents selected from the group consisting of halogen, hydroxy, carboxy, nifro, cyano, amino, Q_6 alkylamino, di(Q.6 alkyl)amino, Q_6 alkoxycarbonyl, Q_6 alkanoyl, Q_6 alkanoylamino, carbamoyl, Q_6 alkylcarbamoyl, Q_6 allcyl optionally substitated by cyano, Q_6 alkoxycarbonyl, or mono-, di-, or tri- halogen, Q_6 alkoxy optionally substitated by mono-, di-, or tri- halogen and Ci-e alkylthio optionally substitated by mono-, di-, or tri- halogen; phenyl substitated by heteroaryl, or heteroaryloxy, wherein said heteroaryl and heteroaryloxy are optionally substituted with one or more substitaents selected from the group consisting of halogen, hydroxy, carboxy, nifro, cyano, amino, Q_6 alkylamino, di(Q.β alkyl)amino, Q.6 alkoxycarbonyl, Q.β alkanoyl, Q_6 alkanoylamino, carbamoyl, Q_6 alkylcarbamoyl, Q.6 alkyl optionally substituted by cyano, Q.6 alkoxycarbonyl, or mono-, di-, or tri-halogen, Q.6 alkoxy optionally substitated by mono-, di-, or tri- halogen, and Q.6 alkylthio optionally substituted by mono-, di-, or tri- halogen; phenyl fused with heteroaryl, or heterocyclyl, wherein said heteroaryl is optionally substitated with one or more substitaents selected from the group consisting of halogen, hydroxy, carboxy, nitro, cyano, amino, Q.6 allcylamino, di(Q_6 alkyl)amino, Q-6 alkoxycarbonyl, Q_6 alkanoyl, Q_6 alkanoylamino, carbamoyl, Q-6 alkylcarbamoyl, Cι.6 allcyl optionally substitated by cyano, Q_6 alkoxycarbonyl, or mono-, di-, or tri-halogen, Q_6 alkoxy optionally substitated by mono-, di-, or tri- halogen and Q.6 alkylthio optionally substituted by mono-, di-, or tri- halogen; or heteroaryl optionally substitated with one or more substituents selected from the group consisting of halogen, hydroxy, carboxy, nifro, cyano, amino, phenyl, benzyl, Q.6 alkylamino, di(Q.6 alkyl)amino, Q.6 alkoxycarbonyl, Q.6 alkanoyl, Q_6 alkanoylamino, carbamoyl, Q.6 alkylcarbamoyl, Q.6 allcyl optionally substitated by cyano, Q.6 alkoxycarbonyl, or mono-, di-, or tri-halogen, Q.6 alkoxy optionally substitated by mono-, di-, or tri- halogen and Q.6 alkylthio optionally substitated by mono-, di-, or tri- halogen.
4. Compound of formula (A) according to claim 1, with the formula (I), their tautomeric and stereoisomeric form, and salts thereof:
Figure imgf000138_0001
wherein
R1 represents aryl or heteroaryl, wherein said aryl and heteroaryl are optionally substitated with one or more substituents selected from the group consisting of halogen, nifro, hydroxy, carboxy, amino, Q.6 allcylamino, di(Q.6 alkyl)amino, C3.8 cycloalkylamino, Q_6 alkoxycarbonyl, phenyl (which phenyl is optionally substitated by halogen, trifluoromethyl, xrifluoromethoxy, nifro, hydroxy, carboxy, amino, Q.6 alkylamino, di(Q.6 alkyl)amino, C3-8 cycloalkylamino, or Cι-6 alkoxycarbonyl), benzyl (in which phenyl moiety is optionally substitated by halogen, nifro, hydroxy, carboxy, amino, Q.6 alkylamino, di(Q-6 alkyl)amino, C3.8 cycloalkylamino, or Q.6 alkoxycarbonyl), sulfonamide, Cι.6 alkanoyl, Q.e alkanoylamino, carbamoyl, Cι.6 alkylcarbamoyl, cyano, Q_6 allcyl (which alkyl is optionally substitated by cyano, nitro, hydroxy, carboxy, amino, Q.6 alkoxycarbonyl or mono-, di-, or tri-halogen),
Q_6 alkoxy (which alkoxy is optionally substitated by mono-, di-, or tri- halogen), phenoxy (in which phenyl moiety is optionally substituted by halogen, nitro, hydroxy, carboxy, amino, Q.6 allcylamino, di(Q_6 alkyl)amino, Q_8 cycloalkylamino, Q.6 alkoxycarbonyl or Q.6 alkyl), Q.6 alkylthio (which alkylthio is optionally substituted by mono-, di-, or tri- halogen), C3.8 cycloalkyl, and heterocycle;
Q.e alkyl optionally substitated by Rπ, OR12, SR12 or N(R12)(R13),
wherein
Rn represents aryl or heteroaryl, wherein said aryl and heteroaryl are optionally substitated with one or more substitaents selected from the group consisting of halogen, nifro, hydroxy, carboxy, amino, Q.β alkylamino, di(Q.6 alkyl)amino, C3.8 cycloalkylamino, Q.6 alkoxycarbonyl, phenyl (which phenyl is optionally substitated by halogen, nitro, hydroxy, carboxy, amino, Q_6 allcylamino, di(Q.6 alkyl)amino, C3.8 cycloalkylamino, or Q_6 alkoxycarbonyl), benzyl (in which phenyl moiety is optionally substitated by halogen, nifro, hydroxy, carboxy, amino, Q.6 alkylamino, di(Q_6 alkyl)amino, C3.8 cycloalkylamino, or Q_6 alkoxycarbonyl), sulfonamide, Q.6 alkanoyl, Q.6 alkanoylamino, carbamoyl, Q.6 alkylcarbamoyl, cyano, Q.6 allcyl (which allcyl is optionally substitated by cyano, nitro, hydroxy, carboxy, amino, Q.6 alkoxycarbonyl or mono-, di-, or tri-halogen), Q-6 alkoxy (which alkoxy is optionally substitated by mono-, di-, or tri- halogen), phenoxy (in which phenyl moiety is optionally substitated by halogen, nifro, hydroxy, carboxy, amino, Q_6 alkylamino, di(Q.6 alkyl)amino, C3.8 cycloalkylamino, Q.6 alkoxycarbonyl or Q.6 alkyl), Q.6 alkylthio (which alkylthio is optionally substitated by mono-, di-, or tri- halogen), C3_8 cycloalkyl, and heterocycle; R12 represents aryl, heteroaryl, or Q.6 alkyl optionally substitated by aryl or heteroaryl, wherein said aryl and heteroaryl are optionally substituted with one or more substitaents selected from the group consisting of halogen, nitro, hydroxy, carboxy, amino, Q_6 alkylamino, di(Q.6 alkyl)amino, C3.8 cycloallcylamino, Ci-e alkoxycarbonyl, phenyl (which phenyl is optionally substitated by halogen, nitro, hydroxy, carboxy, amino, Q_6 alkylamino, di(Q_6 alkyl)amino, Q.8 cycloallcylamino, or Q.6 alkoxycarbonyl), benzyl (in which phenyl moiety is optionally substitated by halogen, nitro, hydroxy, carboxy, amino, Q_6 alkylamino, di(Q.6 alkyl)amino, C3.8 cycloallcylamino, or Ci.6 alkoxycarbonyl), sulfonamide, Q_6 alkanoyl, Q.β alkanoylamino, carbamoyl, Q_6 alkylcarbamoyl, cyano, Q.6 allcyl (which alkyl is optionally substitated by cyano, nifro, hydroxy, carboxy, amino, Q_6 alkoxycarbonyl or mono-, di-, or tri-halogen), Q.6 alkoxy (which alkoxy is optionally substituted by mono-, di-, or tri- halogen), phenoxy (in which phenyl moiety is optionally substitated by halogen, nifro, hydroxy, carboxy, amino, Q_6 alkylamino, di(Q.6 alkyl)amino, C3_8 cycloalkylamino, Q.6 alkoxycarbonyl or Q.6 allcyl), Q_6 alkylthio (which alkylthio is optionally substitated by mono-, di-, or tri- halogen), C3_8 cycloalkyl, and heterocycle; and
R13 represents hydrogen, or Q.6 allcyl;
or
C3.8cycloalkyl optionally fused by aryl,
wherein
said aryl is optionally substitated with one or more substitaents selected from the group consisting of halogen, nifro, hydroxy, carboxy, amino, Q_6 alkylamino, di(Q.6 alkyl)amino, Q_8 cycloallcylamino, .6 alkoxycarbonyl, phenyl (which phenyl is optionally substituted by halogen, nifro, hydroxy, carboxy, amino, Q_6 alkylamino, di(Q.6 alkyl)amino, C3.8 cycloallcylamino, or Q.6 alkoxycarbonyl), benzyl (in which phenyl moiety is optionally substituted by halogen, nifro, hydroxy, carboxy, amino, Q_6 alkylamino, di(Q.6 alkyl)amino, Q_8 cycloalkyl- amino, or Q_6 alkoxycarbonyl), sulfonamide, Q.6 alkanoyl, Q_6 alkanoylamino, carbamoyl, Cι_6 alkylcarbamoyl, cyano, Q.6 alkyl (which alkyl is optionally substitated by cyano, nitro, hydroxy, carboxy, amino, Q.6 alkoxycarbonyl or mono-, di-, or tri-halogen), Q.6 alkoxy (which alkoxy is optionally substituted by mono-, di-, or tri- halogen), phenoxy (in which phenyl moiety is optionally substitated by halogen, nitro, hydroxy, carboxy, amino, Q_6 allcylamino, di(Q_6 alkyl)amino, Q_8 cycloalkylamino, Q_6 alkoxycarbonyl or Q_6 allcyl), Q.6 alkylthio (which alkylthio is optionally substitated by mono-, di-, or tri- halogen), C3.8 cycloalkyl, and heterocycle.
Compound of formula (A) according to claim 1, with the formula (I), their tautomeric and stereoisomeric form, and salts thereof:
Figure imgf000141_0001
wherein
m represents 0, 1, 2, or 3;
p represents 0 or 1;
-X- represents a bond, -O- or -N(R!)- (wherein R1 is hydrogen or Q.6 allcyl); with the proviso that when m is 0, -X- represents a bond.
Qi, Q2 and Q3 independently represent Ν or CH, with the proviso that at least one of Qb Q2 and Q3 is Ν;
R represents aryl or heteroaryl, wherein said aryl and heteroaryl are optionally substitated with one or more substitaents independently selected from the group consisting of halogen, nitro, hydroxy, carboxy, amino, Q.6 allcylamino, di(Q.6 alkyl)amino, C3.8 cycloalkylamino, Q.6 alkoxycarbonyl, phenyl (which phenyl is optionally substitated by halogen, nifro, hydroxy, carboxy, amino, Q_6 alkylamino, di(Q.6 alkyl)amino, C3.8 cycloalkylamino, or Q.6 alkoxycarbonyl), benzyl (in which phenyl moiety is optionally substituted by halogen, nitro, hydroxy, carboxy, amino, Q_6 allcylamino, di(Q_6 alkyl)amino, Q_8 cycloalkylamino, or Q_6 alkoxycarbonyl), sulfonamide, Q_6 alkanoyl, Cι-6 alkanoylamino, carbamoyl, Q_6 alkylcarbamoyl, cyano, Q.6 allcyl (which allcyl is optionally substitated by cyano, nifro, hydroxy, carboxy, amino, Q.6 alkoxycarbonyl or mono-, di-, or tri-halogen), Q_6 alkoxy (which alkoxy is optionally substituted by mono-, di-, or tri- halogen), phenoxy (in which phenyl moiety is optionally substitated by halogen, nitro, hydroxy, carboxy, amino, Q.6 alkylamino, di(Q_6 alkyl)amino, C3.8 cycloalkylamino, or Q.6 alkoxycarbonyl or Q_6 alkyl), Q.6 alkylthio (which alkylthio is optionally substitated by mono-, di-, or tri- halogen), C3.8 cycloalkyl, and heterocycle.
6. A medicament comprising the compound of the formula (A), its tautomeric or stereoisomeric form, or a physiologically acceptable salt thereof as claimed in claim 1 as an active ingredient.
7. The medicament as claimed in claim 6, further comprising one or more pharmaceutically acceptable excipients.
8. The medicament as claimed in claim 6, wherein said compound of the formula (A), its tautomeric or stereoisomeric form, or a physiologically acceptable salt thereof is a VRl antagonist.
9. The medicament as claimed in claim 6 for the treatment and/or prevention of an urological disorder or disease.
10. The medicament as claimed in claim 9, wherein said urological disorder or disease is detrusor overactivity (overactive bladder), urinary incontinence, neurogenic detrusor oeractivity (detrusor hyperflexia), idiopathic detrusor overactivity (detrusor instability), benign prostatic hypeφlasia, and lower urinary tract symptoms.
11. The medicament as claimed in claim 6 for the freatment and/or prevention of pain.
12. The medicament as claimed in claim 11, wherein said pain is chronic pain, neuropathic pain, postoperative pain, or rheumatoid arthritic pain.
13. The medicament as claimed in claim 6 for the freatment and/or prevention of a disorder or disease related to pain.
14. The medicament as claimed in claim 13, wherein said disorder or disease realted to pain is neuralgia, neuropathies, algesia, nerve injury, ischaemia, neurodegeneration, or stroke.
15. The medicament as claimed in claim 6 for the freatment and/or prevention of an inflammatory disorder or disease.
16. The medicament as claimed in claim 15, wherein said inflammatory disorder or disease is asthma or COPD.
17. Use of compounds according to claim 1 for manufacturing a medicament for the freatment and/or prevention of an urological disorder or disease.
18. Use of compounds according to claim 1 for manufacturing a medicament for the freatment and/or prevention of pain.
19. Use of compounds according to claim 1 for manufacturing a medicament for the freatment and/or prevention of an inflammatory disorder or disease.
20. Process for controlling an urological disorder or disease in humans and animals by adminisfration of a VRl-antagonistically effective amoxmt of at least one compound according to claim 1.
21. Process for controlling pain in humans and animals by administration of a VRl- antagonistically effective amount of at least one compound according to claim 1.
22. Process for controlling an inflammatory disorder or disease in humans and animals by adminisfration of a VRl-antagonistically effective amount of at least one compound according to claim 1.
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