WO2005039769A1 - Multilayer sample processing devices and methods - Google Patents
Multilayer sample processing devices and methods Download PDFInfo
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- WO2005039769A1 WO2005039769A1 PCT/US2004/026546 US2004026546W WO2005039769A1 WO 2005039769 A1 WO2005039769 A1 WO 2005039769A1 US 2004026546 W US2004026546 W US 2004026546W WO 2005039769 A1 WO2005039769 A1 WO 2005039769A1
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- cover sheet
- process chamber
- major surface
- control layer
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/12—Specific details about manufacturing devices
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0812—Bands; Tapes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0887—Laminated structure
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/16—Surface properties and coatings
- B01L2300/168—Specific optical properties, e.g. reflective coatings
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
- G01N21/0303—Optical path conditioning in cuvettes, e.g. windows; adapted optical elements or systems; path modifying or adjustment
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/807—Apparatus included in process claim, e.g. physical support structures
- Y10S436/809—Multifield plates or multicontainer arrays
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T156/00—Adhesive bonding and miscellaneous chemical manufacture
- Y10T156/10—Methods of surface bonding and/or assembly therefor
- Y10T156/1002—Methods of surface bonding and/or assembly therefor with permanent bending or reshaping or surface deformation of self sustaining lamina
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T156/00—Adhesive bonding and miscellaneous chemical manufacture
- Y10T156/10—Methods of surface bonding and/or assembly therefor
- Y10T156/1052—Methods of surface bonding and/or assembly therefor with cutting, punching, tearing or severing
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T156/00—Adhesive bonding and miscellaneous chemical manufacture
- Y10T156/10—Methods of surface bonding and/or assembly therefor
- Y10T156/1052—Methods of surface bonding and/or assembly therefor with cutting, punching, tearing or severing
- Y10T156/1082—Partial cutting bonded sandwich [e.g., grooving or incising]
Definitions
- the devices typically include a number of wells or process chambers in which the processing is performed. Detection of various analytes or process products may be performed by detecting signal light emitted from the process chambers.
- the signal light may be caused by, e.g., reactions within the process chambers. In other instances, the signal light may be in response to excitation by interrogating light directed into the process chamber from an external source (e.g., a laser, etc.), where the signal light results from, e.g., chemiluminescence, etc.
- cross-talk can lead to erroneous results when, for example, the second process chamber would not emit any signal light alone, but the signal light transmitted to the second process chamber from the first process chamber is detected and recorded as a false positive result.
- Attempts to avoid cross-talk may include increasing the distance between the process chambers such that any signal light reaching the second process chamber is too weak to register as a positive result with a detector.
- Other approaches include masking or shrouding the process chambers using an external device located over the process chambers such as is described in International Publication No. WO 02/01180 A2 (Bedingham et al.).
- One problem with these approaches is that process chamber density on a device may be limited, resulting in a less than desired number of tests being performed on a given sample processing device.
- Another potential problem with these approaches is that they require the use of articles or materials (e.g., masks, shrouds, etc.) in addition to the sample processing devices, thus increasing the cost and complexity of using the sample processing devices.
- process chambers may be interrogated serially (i.e., one at a time) or only selected groups of process chambers may be interrogated at the same time.
- the control over interrogating light may require the use of masks or shrouds, thus raising the same problems of limited process chamber density, as well as the cost and complexity added by the additional articles/process steps.
- Other problems associated with processing devices include control over the feature size, shape, and location. For example, it may be desired that variations in process chamber sizes, shapes, locations, etc., as well as the size, shape and location of other features in the devices (e.g., delivery conduits, loading chambers, etc.) be limited.
- Variations in feature size may detrimentally affect test accuracy by, e.g., changing the volume of analyte in the different process chambers. Further, variations in feature size may require additional sample volume to, e.g., ensure filling of all process chambers, etc. Variations in feature shape may, e.g., affect the signal light density emitted from a process chamber. Variations in feature location may, e.g., reduce test accuracy if process chamber location is not repeatable between different processing devices.
- the present invention provides sample processing devices that include transmissive layers and control layers to reduce or eliminate cross-talk between process chambers in the processing device.
- the transmissive layers preferably transmit significant portions of signal light and/or interrogation light while the control layers block significant portions of signal light and/or interrogation light.
- the present invention also provides methods of manufacturing processing devices that include transmissive layers and control layers. The methods may, in some embodiments, involve continuous forming processes including co-extrusion of materials to form the transmissive layer and control layer in a processing device, followed by formation of the process chambers in the control layer. In other embodiments, the methods may involve extrusion of materials for the control layer, followed by formation of process chambers in the control layer.
- control layers used in processing devices and methods of the present invention are provided to block the transmission of selected light where the "selected light" may be light of one or more particular wavelengths, one or more ranges of wavelengths, one or more polarization states, or combinations thereof.
- "blocking" of light involves one or more of absorption, reflection, refraction, or diffusion of the selected light.
- transmission of the signal light through the control layer is preferably prevented or reduced to levels that will not result in false positive readings from process chambers.
- transmission of the interrogation light through the control layer is preferably prevented or reduced to levels that will not result in unwanted interrogation of process chambers.
- the control layers may block light of selected wavelengths or ranges of wavelengths.
- the control layers may also block light of one or more selected polarization states (e.g., s polarization, p polarization, circular polarization, etc).
- the term "light” will be used to refer to electromagnetic energy, whether visible to the human eye or not. It may be preferred that the light fall within a range of ultraviolet to infrared electromagnetic energy, and, in some instances, it may be preferred that light include electromagnetic energy in the spectrum visible to the human eye.
- the processing devices of the present invention may provide a number of potential advantages. For example, by blocking the transmission of signal light, cross-talk during emission of signal light can be reduced or eliminated.
- the methods of the present invention may provide for fast and economical manufacturing of processing devices including both transmissive layers and control layers. Further, the methods may provide processing devices including features (e.g., process chambers, distribution conduits, etc.) that are accurately sized shaped and located.
- the present invention provides a sample processing device including a body with a transmissive layer that transmits selected light and a control layer that blocks the selected light, wherein the control layer is attached to the transmissive layer with a first major surface of the control layer facing the transmissive layer and a second major surface facing away from the transmissive layer; a plurality of process chamber structures formed in the control layer, wherein each of the process chamber structures includes an interior window surface and an interior side surface formed by the control layer; a cover sheet attached to the second major surface of the control layer, wherein the cover sheet and the plurality of process chamber structures define a plurality of process chambers in the sample processing device, wherein the selected light can be transmitted into or out of each process chamber through the interior window surface; and a conduit in the sample processing device, wherein each process chamber of the plurality of process chambers is in fluid communication with the conduit.
- the present invention provides a sample processing device including a body with a transmissive layer that transmits selected light and a control layer that blocks the selected light, wherein a first major surface of the control layer faces and is melt-bonded to the transmissive layer, and wherein a second major surface of the control layer faces away from the transmissive layer; a plurality of process chamber structures formed in the body, wherein each of the process chamber structures includes a void formed through the first major surface and the second major surface of the control layer, wherein the void exposes an interior window surface formed by the transmissive layer within each process chamber structure; a cover sheet attached to the second major surface of the control layer, wherein the cover sheet and the plurality of process chamber structures define a plurality of process chambers in the sample processing device, and wherein the cover sheet blocks the selected light; and a conduit formed between the cover sheet and the control layer in the sample processing device, wherein each process chamber of the plurality of process chambers is in fluid communication with the conduit.
- the present invention provides a method of manufacturing a sample processing device, the method including providing a body that includes a transmissive layer that transmits selected light; a control layer that blocks the selected light, wherein the control layer is attached to the transmissive layer with a first major surface of the control layer facing the transmissive layer and a second major surface facing away from the transmissive layer; a plurality of process chamber structures formed in the control layer, wherein each of the process chamber structures includes an interior window surface and an interior side surface formed by the control layer; and attaching a cover sheet to the second major surface of the control layer, wherein the cover sheet and the plurality of process chamber structures define a plurality of process chambers in the sample processing device, and wherein attaching the cover sheet forms a conduit in the sample processing device, wherein each process chamber of the plurality of process chambers is in fluid communication with the conduit.
- the present invention provides a sample processing device including a body with a first major surface and a second major surface, wherein the second major surface is flat, and wherein the body blocks selected light; a plurality of process chamber structures formed in the body, wherein the process chamber structures are formed into the first major surface of the body; a cover sheet attached to the first major surface of the body, wherein the cover sheet and the plurality of process chamber structures define a plurality of process chambers in the sample processing device, and wherein the cover sheet transmits the selected light; and a conduit located between the body and the cover sheet, wherein each process chamber of the plurality of process chambers is in fluid communication with the conduit.
- the present invention provides a method of manufacturing a sample processing device, the method including providing a body that includes a first major surface and a second major surface, wherein the second major surface is flat, and wherein the body blocks selected light, wherein the body further includes a plurality of process chamber structures formed in the first major surface of the body; and attaching a cover sheet to the first major surface of the body, wherein the cover sheet and the plurality of process chamber structures define a plurality of process chambers in the sample processing device; and wherein attaching the cover sheet forms a conduit in the sample processing device, wherein each process chamber of the plurality of process chambers is in fluid communication with the conduit.
- FIG. 1 is a perspective view of one illustrative processing device according to the present invention with the cover removed to expose the structures formed therein.
- FIG. 2 is an enlarged view of a portion of the processing device of FIG. 1.
- FIG. 3 is a cross-sectional view of FIG. 2 taken along line 3-3 in FIG. 2, with the cover located thereon.
- FIG. 4 is a cross-sectional view of an alternative processing device according to the present invention.
- FIG. 5 is a schematic diagram of a portion of one manufacturing process according to the present invention.
- FIG. 6 is a schematic diagram of a system for manufacturing processing devices according to the present invention.
- FIG. 7 is a schematic diagram of a portion of another manufacturing process according to the present invention.
- FIG. 8 is a schematic diagram of a system for manufacturing processing devices according to the present invention.
- the present invention provides a sample processing device that can be used in the processing of liquid sample materials (or sample materials entrained in a liquid) in multiple process chambers to obtain desired reactions, e.g., PCR amplification, ligase chain reaction (LCR), self-sustaining sequence replication, enzyme kinetic studies, homogeneous ligand binding assays, and other chemical, biochemical, or other reactions that may, e.g., require precise and/or rapid thermal variations. More particularly, the present invention provides sample processing devices that include one or more process arrays, each of which include a loading chamber, a plurality of process chambers and a main conduit placing the process chambers in fluid communication with the loading chamber.
- desired reactions e.g., PCR amplification, ligase chain reaction (LCR), self-sustaining sequence replication, enzyme kinetic studies, homogeneous ligand binding assays, and other chemical, biochemical, or other reactions that may, e.g., require precise and/or rapid thermal variations.
- the present invention provides sample processing
- sample processing devices of the present invention may be similar to those described in, e.g., International Publication Nos. WO 02/01180 and WO 02/00347 (both to Bedingham et al.).
- the documents identified above all disclose a variety of different features that could be incorporated into sample processing devices of the present invention.
- FIGS. 1 & 2 One illustrative sample processing device manufactured according to the principles of the present invention is illustrated in FIGS. 1 & 2, where FIG. 1 is a perspective view of one sample processing device 10 and FIG. 2 is an enlarged plan view of a portion of the sample processing device 10. In both views, a cover (described below in connection with FIG. 3) has been removed to expose structures formed in the body 60 of the device 10.
- the sample processing device 10 includes at least one, and preferably a plurality of process arrays 20. Each of the depicted process arrays 20 preferably extends from proximate a first end 12 towards the second end 14 of the sample processing device 10.
- the process arrays 20 are depicted as being substantially parallel in their arrangement on the sample processing device 10. Although this arrangement may be preferred, it will be understood that any arrangement of process arrays 20 may alternatively be preferred. Alignment of the process arrays 20 as depicted may be useful if the main conduits 40 of the process arrays are to be closed simultaneously as discussed in, e.g., International
- Each of the process arrays 20 in the depicted embodiment includes at least one main conduit 40, and a plurality of process chambers 50 located along each main conduit 40.
- the process arrays 20 may also preferably include a loading structure in fluid communication with a main conduit 40 to facilitate delivery of sample material to the process chambers 50 through the main conduit 40. It may be preferred that, as depicted in FIG. 1, each of the process arrays include only one loading structure 30 and only one main conduit 40.
- the loading structure 30 may be designed to mate with an external apparatus (e.g., a pipette, hollow syringe, or other fluid delivery apparatus) to receive the sample material.
- the loading structure 30 itself may define a volume or it may define no specific volume, but, instead, be a location at which sample material is to be introduced.
- the loading structure may be provided in the form of a port through which a pipette or needle is to be inserted.
- the loading structure may be, e.g., a designated location along the main conduit that is adapted to receive a pipette, syringe needle, etc.
- the loading may be performed manually or by an automated system (e.g., robotic, etc.).
- the processing device 10 may be loaded directly from another device (using an automated system or manually).
- the loading chamber depicted in FIG. 1 is only one embodiment of a loading structure 30 in fluid communication with the main conduit 40. It may be preferred that the loading chamber volume, i.e., the volume defined by the loading chamber (if so provided), be equal to or greater than the combined volume of the main conduit 40, process chambers 50, and feeder conduits 42 (if any).
- the process chambers 50 are in fluid communication with the main conduit 40 through feeder conduits 42.
- the loading structure 30 in each of the process arrays 20 is in fluid communication with each of the process chambers 50 located along the main conduit 40 leading to the loading structure 30.
- each of the process arrays 20 may also include an optional drain chamber (not shown) located at the end of the main conduit 40 opposite the loading structure 30.
- FIG. 3 is a cross-sectional view of the portion of the processing device 10 depicted in FIG. 2 taken along line 3-3 in FIG. 2.
- the processing device 10 includes a body 60 that includes a transmissive layer 62 and control layer 64. It may be preferred that the transmissive layer 62 and control layer 64 be attached by a melt bond.
- a transmissive layer 62 and control layer 64 be attached by a melt bond.
- a melt bond As used herein, a
- melt bond is a bond formed by the melting and/or mixing of materials such as that occurring during, e.g., heat sealing, thermal welding, ultrasonic welding, chemical welding, solvent bonding, coextrusion, extrusion casting, etc.
- the materials facing each other in transmissive layer 62 and control layer 64 must be compatible with melt bonding so that an attachment of sufficient integrity can be formed to withstand the forces experienced during processing of sample materials in the process chambers.
- the transmissive layer 62 and control layer 64 may be attached to each other using, e.g., adhesives, combinations of melt bonding and adhesives, etc. Examples of some attachment techniques may be described in, e.g., International Publication No. WO 02/01180.
- the transmissive layer 62 is preferably constructed of one or more materials such that the transmissive layer 62 transmits significant portions of selected light.
- significant portions may be, e.g., 50% or more of normal incident selected light, more preferably 75% or more of normal incident selected light.
- the selected light may be light of one or more particular wavelengths, one or more ranges of wavelengths, one or more polarization states, or combinations thereof.
- transmissive layer 62 examples include, but are not limited to, e.g., polypropylenes, polyesters, polycarbonates, polyethylenes, polypropylene-polyethylene copolymers, cyclo-olefin polymers (e.g., polydicyclopentadiene), etc.
- the control layer 64 is preferably constructed of one or more materials such that the control layer 64 blocks significant portions of selected light.
- significant portions of blocked light may be, e.g., 50% or more of normal incident selected light, more preferably 75% or more of normal incident selected light, and even more preferably 90% or more of normal incident selected light.
- the selected light may be one or more particular wavelengths, one or more ranges of wavelengths, one or more polarization states, or combinations thereof.
- suitable materials for the control layer 64 include, but are not limited to, e.g., polypropylenes, polyesters, polycarbonates, polyethylenes, polypropylene- polyethylene copolymers, cyclo-olefin polymers (e.g., polydicyclopentadiene), etc., that have been modified to provide the desired light blocking function.
- the material used for the control layer 64 may include a light blocking filler (e.g., colorants, carbon black, metallic particles, etc.) to prevent or reduce transmission of selected light through the control layer 64.
- control layer 64 may include a coating or other treatment that provides the desired light blocking function.
- a melt bond between transmissive layer 62 and control layer 64 it may be preferred that the transmissive layer 62 and the control layer 64 be formed of compatible materials, in some instances, it may be preferred that the transmissive layer 62 and the control layer 64 be formed of the same polymeric material.
- the processing device 10 includes process chambers 50 and a main conduit 40 located between the body 60 and the cover sheet 70.
- Cover sheet 70 is attached to the surface 65 of the control layer 64 facing away from the transmissive layer 62 using adhesive 72.
- the cover sheet 70 may, however, be attached to the control layer 64 by any suitable technique or combination of techniques, e.g., adhesives, combinations of melt bonding and adhesives, etc. Examples of some suitable attachment techniques may be described in, e.g., International Publication No. WO 02/01180.
- the process chambers 50 are, in the depicted embodiment, defined by process chamber structures formed in the control layer 64 of the body 60 and the cover sheet 70 attached to the control layer 64.
- the process chamber structures are in the form of voids formed through the control layer 64, the voids including interior side surfaces 52.
- An interior window surface 54 is also formed within the process chamber 50 by a portion of the transmissive layer 62 exposed by the void in the control layer 64.
- the process chambers 50 may be described as having a height measured from the cover sheet 70 to the interior window surface 54.
- the control layer 64 may have a thickness between the cover sheet 70 and the transmissive layer 62 that is less than or equal to the height of each process chamber 50. If the height of the process chamber 50 is greater than the thickness of the control layer 64, the transmissive layer 62 may preferably be thinned or otherwise deformed in the area of the window surface 54.
- the process chamber structure is formed as a void that exposes a portion of the transmissive layer 62 within the interior window surface 54, it should be understood that, in some instances, it may be acceptable if a relatively thin portion of the control layer 64 remains in the area occupied by the window surface 54. Any such relatively thin portion of the control layer 64 should, however, be sufficiently thin such that transmission of the selected light through the window surface 54 is still possible.
- the process chamber structure also preferably includes one or more interior side surfaces 52 as depicted that are formed by the control layer 64. The interior side surfaces
- FIG. 3 also depicts that the process chambers 50 may include a reagent 58 located therein. It may be preferred that at least some, and preferably all, of the process chambers 50 in the devices 10 of the present invention contain at least one reagent before the cover sheet 70 is attached to the body 60.
- the reagent 58 may be fixed within the process chamber 50 as depicted in FIG. 3 or it may be loose within the process chamber 50.
- the reagent 58 is optional, i.e., sample processing devices 10 of the present invention may or may not include any reagents 58 in the process chambers 50.
- some of the process chambers 50 may include a reagent 58, while others do not.
- different process chambers 50 may contain different reagents (in which case it may be desirable to fix the reagents to a surface within each process chamber 50).
- the interior of the process chamber structures may be coated or otherwise processed to control the adhesion of reagents 58.
- reagents may be provided on the cover sheet 70.
- the cover sheet 70 is depicted as generally flat, it should be understood that the cover sheet 70 may deviate from a flat sheet if so desired.
- the cover sheet 70 may be formed to increase or decrease the volume of the process chamber 50.
- the cover sheet 70 may be provided as, e.g., a sheet of metal foil, polymeric material, multi-layer composite, etc. that is attached to the control layer 64 over the process chamber structures 52 to form process chambers 50.
- cover sheet 70 is depicted as a unitary homogenous layer, it should be understood that the cover sheet 70 may include one or materials and those materials may be provided in different layers or they may be intermixed within the cover sheet 70. If the cover sheet 70 includes a metallic foil, the cover sheet 70 may preferably include a passivation layer on the surfaces that face the interiors of the process chambers 50 and main conduit 40 to prevent contamination of the sample materials by the metal. As an alternative to a separate passivation layer, any adhesive layer 72 used to attached the cover sheet 70 to the control layer 64 may also serve as a passivation layer to prevent contact between the sample materials and any metallic layer in the cover sheet 70.
- the cover sheet 70 may preferably be deformable such that the main conduits 40 can be closed or sealed as described in, e.g., International Publication No. WO 02/01180.
- the cover sheet 70 and adhesive 72 (if present) provide a reflective surface facing the interior of the process chambers 50 for the selected light.
- the reflectivity may be provided by, e.g., a metallic surface of the cover sheet 70, by the adhesive 72 itself, or by a reflective polymeric sheet used to form the cover sheet 70, or any other reflective structure or material.
- the process chambers 50 may be interrogated by light that may be absorbed by one or more components within the process chamber 50.
- a detector could be positioned on the side of the processing device opposite from the side at which the selected light is directed into the process chamber 50. For example, if the selected light is directed into the process chamber 50 through the transmissive layer 62, the detector may be placed on the same side as the cover 70 such that the selected light is either absorbed within the process chamber 50 or transmitted through the transmissive layer 62, process chamber 50 and cover 70 before reaching the detector. Such absorption may be used to determine the presence or absence of one or more analytes within the process chamber 50.
- signal light from, e.g., chemiluminescence in the process chamber 50 may be transmitted out of the process chamber 50 through either the transmissive layer 62 or the transmissive cover 70.
- a variety of adhesives 72 may be used, although any adhesive selected should be capable of withstanding the forces generated during processing of any sample materials located in the process chambers 50, e.g., forces developed during distribution of the sample materials, forces developed during thermal processing of the sample materials, etc. Those forces may be large where, e.g., the processing involves thermal cycling as in, e.g., polymerase chain reaction and similar processes.
- any adhesives used in connection with the sample processing devices exhibit low fluorescence, be compatible with the processes and materials to be used in connection with sample processing devices, e.g. PCR, etc. It may be preferred to use adhesives that exhibit pressure sensitive properties. Such adhesives may be more amenable to high volume production of sample processing devices since they typically do not involve the high temperature bonding processes used in melt bonding, nor do they present the handling problems inherent in use of liquid adhesives, solvent bonding, ultrasonic bonding, and the like. Techniques for identifying and selecting pressure sensitive adhesives are discussed in, e.g., International Publication No. WO 02/01180.
- the pressure sensitive adhesives used in connection with the sample processing devices of the present invention include materials which ensure that the properties of the adhesive are not adversely affected by water.
- the pressure sensitive adhesive will preferably not lose adhesion, lose cohesive strength, soften, swell, or opacity in response to exposure to water during sample loading and processing.
- the pressure sensitive adhesive should not contain any components which may be extracted into water during sample processing, thus possibly compromising the device performance.
- the pressure sensitive adhesive it may be preferred that the pressure sensitive adhesive be composed of hydrophobic materials. As such, it may be preferred that the pressure sensitive adhesive be composed of silicone materials.
- FIG. 3 Also depicted in the embodiment of FIG. 3 is a cross-sectional view of the main conduit 40 that is in fluid communication with the process chambers 50.
- the main conduit 40 in the depicted embodiment, is defined by conduit structure 44 formed in the control layer 64 of the body 60 and the cover sheet 70 attached to the confrol layer 64.
- the conduit structure 44 is in the form of a channel formed into the surface of the control layer 64 facing away from the transmissive layer 62.
- the conduit structure 44 may be described as having a height measured from the cover sheet 70 towards the transmissive layer 62. Furthermore, it may be preferred that the height of the conduit structure 44 be less than the thickness of the control layer 64 (as measured between the opposing major surfaces of the control layer 64) such that, e.g., none of the transmissive layer 62 be exposed within the conduit structure 44.
- the cover sheet 70 is depicted as generally flat, it should be understood that the cover sheet 70 may deviate from a flat sheet if so desired.
- the cover sheet 70 may be formed to increase or decrease the cross-sectional area of the conduit 40 (in the view as seen in FIG. 3).
- FIG. 4 depicts an alternative construction for a sample processing device according to the present invention.
- the processing device is formed by a body 160 and a cover sheet 170.
- the body 160 is preferably formed of a material or materials that block the transmission of selected light between the process chambers 150. Transmission of the selected light into and/or out of the process chambers 150 occurs, instead, through the cover sheet 170 which preferably transmits significant portions of the selected light.
- significant portions may be, e.g., 50% or more of normal incident selected light, more preferably 75% or more of normal incident selected light.
- the selected light may be one or more particular wavelengths, one or more ranges of wavelengths, one or more polarization states, or combinations thereof.
- suitable materials for the cover sheet 170 include, but are not limited to, e.g., polypropylenes, polyesters, polycarbonates, polyethylenes, polypropylene-polyethylene copolymers, cyclo-olefin polymers (e.g., polydicyclopentadiene), etc.
- cover sheet 170 is depicted as a unitary homogenous layer, it should be understood that the cover sheet 170 may include one or materials and those materials may be provided in different layers or they may be intermixed within the cover sheet 170.
- the body 160 is preferably constructed of one or more materials such that the body
- significant portions of blocked light may be, e.g., 50% or more of normal incident selected light, more preferably 75% or more of normal incident select light, and even more preferably 90% or more of normal incident selected light.
- the selected light may be one or more particular wavelengths, one or more ranges of wavelengths, one or more polarization states, or combinations thereof.
- Examples of some suitable materials for the body 160 include, but are not limited to, e.g., polypropylenes, polyesters, polycarbonates, polyethylenes, polypropylene- polyethylene copolymers, cyclo-olefin polymers (e.g., polydicyclopentadiene), etc., that have been modified to provide the desired light blocking function.
- the material used for the body 160 may include a light blocking filler (e.g., colorants, carbon black, metallic particles, etc.) to prevent or reduce transmission of selected light through the body 160.
- the body 160 may include a coating or other treatment that provides the desired light blocking function.
- the processing device includes process chambers 150 and a main conduit 140 located between the body 160 and the cover sheet 170.
- the process chambers 150 are, in the depicted embodiment, defined by process chamber structures 152 formed in the surface 161 of body 160 and the cover sheet 170 attached to the body 160.
- the process chamber structures 152 are in the form of depressions formed into the body, but not through the entire thickness of the body 160.
- the cover sheet 170 is depicted as generally flat, it should be understood that the cover sheet 170 may deviate from a flat sheet if so desired.
- the cover sheet 170 may be formed to increase or decrease the volume of the process chamber 150.
- Cover sheet 170 is attached to the surface 161 of the body 160 using adhesive 172.
- the cover sheet 170 may, however, be attached to the body 160 by any suitable technique or combination of techniques, e.g., adhesives, combinations of melt bonding and adhesives, etc. Examples of some suitable attachment techniques may be described above with respect to adhesive 72 on cover sheet 70 in FIG. 3, as well as in, e.g., International Publication No. WO 02/01180. Also depicted in the embodiment of FIG. 4 is a cross-sectional view of the main conduit 140 that is in fluid communication with the process chambers 150.
- the main conduit 140 in the depicted embodiment, is defined by conduit structure 144 formed in the body 160 and the cover sheet 170 attached to the body 160.
- the conduit structure 144 is in the form of a channel formed into the surface 161 facing the cover sheet 170.
- the conduit structure 144 may be described as having a height measured from the cover sheet 170 towards the opposing surface 163 of the body 160.
- the height of the conduit structure 144 be less than the thickness of the body 160 as measured between opposite major surfaces 161 and 163.
- the processing devices of the present invention may be manufactured by a variety of methods and techniques. A portion of one exemplary manufacturing method is depicted in FIG. 5 for manufacturing processing devices including a body having a transmissive layer and control layer as described in connection with FIG. 3.
- a melt- processable polymer for the transmissive layer 62 is delivered from an extruder 80 to multi-layer feedblock and film die 82.
- a melt-processable polymer for the control layer 64 is delivered from extruder 84 the multi-layer feedblock and film die 82.
- the two melt-processable polymers streams are delivered to the multi-layer feedblock and film die 82 at or above their melt-processing temperature.
- the multi-layer feedblock and film die 82 preferably keeps the two polymer melt streams from extruders 80 and 84 separated such that the two polymers form separate and discrete layers 62 and 64.
- the layers 62 and 64 of polymer are discharged from the multi-layer feedblock and film die 82 onto a forming tool 86.
- the polymers be drop cast onto the surface of the forming tool 86 at a point near the nip between forming tool 86 and backup roll 88.
- the forming tool 86 includes protrusions 87 extending therefrom that correspond to the various features to be formed in the processing devices, e.g., process chambers, conduits, etc. It may be preferred that the thickness of each of layers 62 and 64 be controlled such that the thickness of layer 64 is less than or equal to the height of the protrusions 87 that form the process chamber structures in the processing devices of the present invention. Limiting the thickness of layer 64 in that manner can provide for process chamber structures in layer 64 that are formed as voids as described above with respect to FIG. 3.
- At least the control layer 64 be delivered to the forming tool 86 at a temperature that is at or above its melt processing temperature (i.e., the temperature at which it can be formed or molded).
- the polymer in the confrol layer can adequately form or be replicated to the shape of the protrusions 87 on the forming tool 86.
- the forming tool 86 is depicted as a roll, it should be understood that it may alternatively be provided as a continuous belt or other structure amenable to continuous web-form processing.
- the polymer of the transmissive layer 62 also be delivered to the nip formed by forming tool 86 and backup roll 88 at a temperature that is at or above its melt processing temperature such that the two layers 62 and 64 can be formed together to provide a melt bond between the two layers while the features of forming tool 86 are being formed into the control layer 64.
- the temperatures of both layers 62 and 64 are preferably lowered to below their respective melt processing temperatures at some point after the nip with backup roll 88 to retain the structures formed in layer 64 and provide mechanical stability to the web.
- the result of the processing depicted in FIG. 5 is a web including both a fransmissive layer 62 and control layer 64 to form the body 60 of a processing device according to the present invention.
- FIG. 6 is a schematic diagram of the larger process for manufacturing processing devices according to the present invention.
- the system depicted in FIG. 6 includes the components and process of FIG. 5 as apparatus 89.
- the web formed by apparatus 89 is a schematic diagram of the larger process for manufacturing processing devices according to the present invention.
- FIGS. 7 & 8 depict an alternative manufacturing process that may be used to, e.g., manufacture processing devices constructed as described in FIG. 4.
- the A melt-processable polymer for the body 160 is delivered from an extruder 180 to die 182.
- the melt-processable polymer stream is delivered to the die 182 at or above its melt- processing temperature.
- the polymer layer 160 is discharged from the die 182 onto a forming tool 186. It may be preferred that the layer 160 be drop cast onto the forming tool 186 just before the nip formed with backup roll 188.
- the forming tool 186 includes protrusions 187 extending therefrom that correspond to the various features to be formed in the processing devices, e.g., process chambers, conduits, etc. It is preferred that the thickness of the polymer layer 160 be controlled such that the thickness of layer 160 is greater than the height of the protrusions 187 that form the process chamber structures in the processing devices of the present invention.
- the polymer layer 160 be delivered to the forming tool 186 at a temperature that is at or above its melt processing temperature (i.e., the temperature at which it can be formed or molded).
- the polymer can adequately form or be replicated to the shape of the protrusions 187 on the forming tool 186.
- the forming tool 186 is depicted as a roll, it should be understood that it may alternatively be provided as a continuous belt or other structure amenable to continuous web-form processing.
- the temperature of the polymer layer 160 is preferably lowered to below the melt processing temperature at some point after the nip with backup roll 188 to retain the structures formed in layer 160 and provide mechanical stability to the web.
- the result of the processing depicted in FIG. 7 is a web 160 that can be used to form the bodies of processing devices according to the present invention.
- the web 160 includes structures formed therein for, e.g., process chambers, conduits, loading chambers, etc.
- FIG. 8 is a schematic diagram of the larger process for manufacturing processing devices according to the present invention.
- the system depicted in FIG. 8 includes the components and process of FIG. 7 as apparatus 189.
- the web formed by apparatus 189 may preferably be fed into a reagent station 190 where reagents can be added to, e.g., the process chamber structures as discussed herein. Following the addition of reagents, the web may preferably be fed into a laminating station 191 where a cover sheet web 170 is laminated thereto to form a composite web structure including process chambers, conduits, etc. The composite web may then preferably be delivered to a sheeting station 192 where the web is separated by, e.g., die cutting, into individual processing devices 110.
- the web formed by apparatus 189 may be sheeted before any reagents are added to the sheeted web. It may also be preferred that in such a process, the cover sheet web 170 be sheeted before it is laminated to the sheets formed from the web of apparatus 189.
Abstract
Description
Claims
Priority Applications (7)
Application Number | Priority Date | Filing Date | Title |
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EP04781263A EP1682271B1 (en) | 2003-10-09 | 2004-08-16 | Multilayer sample processing devices and methods |
AU2004283174A AU2004283174B2 (en) | 2003-10-09 | 2004-08-16 | Multilayer sample processing devices and methods |
AT04781263T ATE448876T1 (en) | 2003-10-09 | 2004-08-16 | MULTI-LAYER SAMPLE PROCESSING DEVICES AND METHODS |
CN200480029758.2A CN1867406B (en) | 2003-10-09 | 2004-08-16 | Multilayer processing devices and methods |
JP2006533851A JP2007508542A (en) | 2003-10-09 | 2004-08-16 | Multilayer sample processing apparatus and method |
CA002541900A CA2541900A1 (en) | 2003-10-09 | 2004-08-16 | Multilayer sample processing devices and methods |
DE602004024229T DE602004024229D1 (en) | 2003-10-09 | 2004-08-16 | MULTILAYER SAMPLE PROCESSING DEVICES AND METHOD |
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US10/682,597 US7718133B2 (en) | 2003-10-09 | 2003-10-09 | Multilayer processing devices and methods |
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EP (2) | EP1682271B1 (en) |
JP (2) | JP2007508542A (en) |
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AT (1) | ATE448876T1 (en) |
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CA (1) | CA2541900A1 (en) |
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WO2011063313A1 (en) | 2009-11-23 | 2011-05-26 | 3M Innovative Properties Company | Carrier with flexible microassay device and methods of use |
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CN1867406B (en) | 2010-06-16 |
EP2181766A1 (en) | 2010-05-05 |
ATE448876T1 (en) | 2009-12-15 |
AU2004283174A1 (en) | 2005-05-06 |
US8865091B2 (en) | 2014-10-21 |
JP2007508542A (en) | 2007-04-05 |
EP1682271A1 (en) | 2006-07-26 |
US20100183479A1 (en) | 2010-07-22 |
DE602004024229D1 (en) | 2009-12-31 |
CA2541900A1 (en) | 2005-05-06 |
JP5086381B2 (en) | 2012-11-28 |
US20050079101A1 (en) | 2005-04-14 |
JP2010122237A (en) | 2010-06-03 |
AU2004283174B2 (en) | 2010-01-07 |
CN1867406A (en) | 2006-11-22 |
US7718133B2 (en) | 2010-05-18 |
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