WO2005034850A2 - Cycloalkyl heterocycles for treating hepatitis c virus - Google Patents

Cycloalkyl heterocycles for treating hepatitis c virus Download PDF

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Publication number
WO2005034850A2
WO2005034850A2 PCT/US2004/027533 US2004027533W WO2005034850A2 WO 2005034850 A2 WO2005034850 A2 WO 2005034850A2 US 2004027533 W US2004027533 W US 2004027533W WO 2005034850 A2 WO2005034850 A2 WO 2005034850A2
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Prior art keywords
phenyl
cyclohexyl
methoxy
pyrazol
methyl
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PCT/US2004/027533
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French (fr)
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WO2005034850A3 (en
Inventor
Peter W. Glunz
Brent D. Douty
Scott W. Martin
Jeffrey Romine
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Bristol-Myers Squibb Company
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Priority to AT04782095T priority Critical patent/ATE498398T1/en
Priority to EP04782095A priority patent/EP1663105B1/en
Priority to JP2006526121A priority patent/JP4897484B2/en
Priority to DE602004031435T priority patent/DE602004031435D1/en
Publication of WO2005034850A2 publication Critical patent/WO2005034850A2/en
Publication of WO2005034850A3 publication Critical patent/WO2005034850A3/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D231/00—Heterocyclic compounds containing 1,2-diazole or hydrogenated 1,2-diazole rings
    • C07D231/02—Heterocyclic compounds containing 1,2-diazole or hydrogenated 1,2-diazole rings not condensed with other rings
    • C07D231/10—Heterocyclic compounds containing 1,2-diazole or hydrogenated 1,2-diazole rings not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members
    • C07D231/12—Heterocyclic compounds containing 1,2-diazole or hydrogenated 1,2-diazole rings not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members with only hydrogen atoms, hydrocarbon or substituted hydrocarbon radicals, directly attached to ring carbon atoms
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D231/00—Heterocyclic compounds containing 1,2-diazole or hydrogenated 1,2-diazole rings
    • C07D231/02—Heterocyclic compounds containing 1,2-diazole or hydrogenated 1,2-diazole rings not condensed with other rings
    • C07D231/10—Heterocyclic compounds containing 1,2-diazole or hydrogenated 1,2-diazole rings not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members
    • C07D231/14—Heterocyclic compounds containing 1,2-diazole or hydrogenated 1,2-diazole rings not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms

Abstract

Compounds of Formula (I) are disclosed which inhibit hepatitis C NS5B RNA-dependent RNA polymerase and are useful for treating hepatitis C. Compositions and methods of using these compounds are also disclosed.

Description

CYCLOALKYL HETEROCYCLES FOR TREATING HEPATITIS C VIRUS
CROSS-REFERENCE TO RELATED APPLICATIONS This application claims the benefit of U.S. provisional application No.
60/502,067 filed September 11, 2003.
BACKGROUND OF THE INVENTION This invention is directed to compounds which inhibit the RNA-dependent
RNA polymerase (RdRp) encoded by Hepatitis C virus (HCV). The compounds, or pharmaceutically acceptable salts thereof, are useful for the treatment of HCV viral infections. HCV is a major human pathogen, infecting an estimated 170 million people worldwide. A substantial fraction of these HCV infected individuals develop serious progressive liver disease, including cirrhosis and hepatocellular carcinoma. (Lauer, G. M.; Walker, B. D. N. Engl. J. Med. (2001), 345, 41-52). Presently, the most effective HCV therapy employs a combination of alpha- interferon and ribavirin, leading to sustained efficacy in 40% of patients. (Poynard, T. et al. Lancet (1998), 352, 1426-1432). Recent clinical results demonstrate that pegylated alpha-interferon is superior to unmodified alpha-interferon as monotherapy (Zeuzem, S. et al. N. Engl. J. Med. (2000), 343, 1666-1672). However, even with experimental therapeutic regimens involving combinations of pegylated alpha- interferon and ribavirin, a substantial fraction of patients do not have a sustained reduction in viral load. In addition, the prospects for development of a prophylactic or therapeutic vaccine appear dim, in spite of intensive research efforts. Thus, there is a clear need to develop effective therapeutics for treatment of HCV infection.
HCV is a positive-stranded RNA virus. Based on comparison of deduced amino acid sequence and the extensive similarity in the 5' untranslated region, HCV has been classified as a separate genus in the Flaviviridae family. All members of the Flaviviridae family have enveloped virions that contain a positive stranded RNA genome encoding all known virus-specific proteins via translation of a single, uninterrupted, open reading frame.
Considerable heterogeneity is found within the nucleotide and encoded amino acid sequence throughout the HCV genome. At least six major genotypes have been characterized, and more than 50 subtypes have been described. The major genotypes of HCV differ in their distribution worldwide, and the clinical significance of the genetic heterogeneity of HCV remains elusive despite numerous studies of the possible effect of genotypes on pathogenesis and therapy.
The RNA genome is about 9.6 Kb in length, and encodes a single polypeptide of about 3000 amino acids. The 5' untranslated region contains an internal ribosome entry site (IRES), which directs cellular ribosomes to the correct AUG for initiation of translation. The translated product contains the following proteins: core-El-E2- p7-NS2-NS3-NS4A-NS4B-NS5A-NS5B. This precursor protein is cotranslationally and posttranslationally processed into at least 10 viral structural (core, El, E2) and nonstructural (NS2-NS5B) proteins by the action of host cell signal peptidase and by two distinct viral proteinase activities (NS2/3 and NS3). Although the functions of the NS proteins are not completely defined, it is known that NS3 is a serine protease/RNA helicase, NS4A is a protease cofactor, and NS5B is an RNA dependent RNA polymerase involved in viral replication. It has recently been demonstrated that functional NS5B is required for virus infectivity in chimpanzees (Kolykhalov, A. A. et al. I. Virol. (2000), 74, 2046-2051). Because HCV only infects chimpanzees and humans, this result strongly suggests that inhibition of the NS5B RdRp is a viable approach for the development of HCV therapeutic agents.
DESCRIPTION OF RELATED ART
Efforts toward the development of HCV NS5B RdRp inhibitors have resulted in the following disclosures: Altamura et al. (Istituto Di Ricerche Di Biologia Molecolare) describe diketoacid RdRp inhibitors (WO 00/06529). Altamura et al. suggest that the diketoacids inhibit HCV RdRp by interfering with the binding of phosphoryl groups at the active site of the enzyme.
A series of three disclosures from Viropharma Inc. (Bailey, T. R. et al, WO 00/10573; Bailey , T. R. et al, WO 00/13708; Young, D. C. et al, WO 00/18231) describe HCV RdRp inhibitors. WO 00/10573 covers a series of rhodanine derivatives, WO 00/13708 covers a series of barbituric acid or thiobarbituric acid derivatives, and WO 0018231 covers a series of dihydrobenzothiophene derivatives.
R. Storer (Biochem Pharma, Inc.) has disclosed the use of a series of dioxolane nucleosides for treatment of HCV (WO 01/32153). Hashimoto et al, (Japan Tobacco, Inc.) disclose a series of fused-ring heterocycles as inhibitors of HCV RdRp (WO 01/47883, US 03/0050320), and a similar series of fused-ring heterocycles is also disclosed by Boehringer Ingelheim (WO 02/04425, WO 03/007945, WO 03/010141). ShireBiochem (WO 02/100851) discloses 5-membered carboxylates. Agouron Pharmaceuticals discloses pyran-2-ones (EP 1 256 628). DESCRIPTION OF THE INVENTION
This invention encompasses compounds which are useful for inhibiting hepatitis C NS5B RNA-dependent RNA polymerase and for treating hepatitis C. Pharmaceutical compositions and methods of treating hepatitis C using these compounds are also encompassed.
One aspect of the invention are compounds of Formula (I)
Figure imgf000004_0001
where:
Figure imgf000005_0001
A is CO2R5, CONSO2R5, SO2NCOR5,
Figure imgf000005_0002
Figure imgf000005_0003
Het is pyrazole, imidazole, oxazole, triazole, thiazole, pyrrole, furan, or thiophene;
X is O, S, NR5, or CH2;
R1 is hydrogen, halogen, Chalky!, trifluoromethyl, or phenyl;
R >2 i •s C3- cycloalkyl or C5_ι2 bridged bicycloalkyl;
R is hydrogen, halogen, C1-6alkyl, or C1-6alkoxy;
Figure imgf000005_0004
R >5 i •s hydrogen or C1-6alkyl;
R6 is hydrogen, methyl, or OR5;
R7 is C1-6alkoxy, cyano, trifluoromethyl, -CO2R5, -CONR9R10, SO2R5, or
SO2NR9R10;
R is hydrogen, halogen, Cι-6alkyl, Cι-6alkoxy, cyano, trifluoromethyl, aceto, CO2R or CONR9R10; R9 and R10 are independently hydrogen, C1-6alkyl, -CH2CH2OH; or
NR9R10 taken together form pyrrolidine, piperidine, 4-hydroxypiperidine, piperazine, 4-methylpiperazine, mo holine, or thiomorpholine; and
Ar1 is thiophene or phenyl substituted with 0-3 substituents selected from halogen, C1-6alkyl, C1-6alkoxy, cyano, trifluoromethyl, aceto, CO2R5, and CONR9R10;
and pharmaceutically acceptable salts and solvates of these compounds.
Another aspect of the invention are compounds which have the following 1,3,4 geometric configuration on a 5-member heterocyclic ring:
Figure imgf000006_0001
Another aspect of this invention are compounds of Formula la.
Figure imgf000006_0002
la
Another aspect of this invention are compounds of Formula lb.
Figure imgf000006_0003
lb
Another aspect of this invention are compounds of Formula Ic.
Figure imgf000007_0001
Ic
Another aspect of this invention are compounds of Formula Id.
Figure imgf000007_0002
Id
Another aspect of this invention are compounds of Formula le.
Figure imgf000007_0003
Ie
Another aspect of this invention are compounds of Formula If.
Figure imgf000007_0004
If
Another aspect of this invention are compounds of Formula Ig.
Figure imgf000007_0005
Ig Another aspect of this invention are compounds of Formula Ih. )
Figure imgf000008_0001
Ih
Another aspect of the invention are compounds of Formula I where A is -CO2R5
Another aspect of the invention are compounds of Formula I where A-L is
Figure imgf000008_0002
Another aspect of the invention are compounds of Formula I where R4 is
Figure imgf000008_0003
. For this aspect, we note that a very broad range of substituents at Ar , including hydrogen and heteroaryl moieties, have relatively minor effect on the activity of the compounds. In contrast, activity of the compounds are enhanced when R7 is a polar moiety.
Some bridged bicycloalkyl groups include bicyclo[l.l.l]pentane, bicyclo[2.1.1]hexane, bicyclo[2.2.1]heptane, bicyclo[2.2.2]octane, bicyclo [3.1.1] heptane, bicyclo[3.2.1]octane, bicyclo[3.3.1]nonane, bicyclo[3.3.3]undecane, and adamantane.
Some compounds of the invention include the following: (1) ethyl l-cyclohexyl-5-(4-benzyloxyphenyl)-lH-pyrazole-3-carboxylate; (2) methyl (2E)-3- { 5- [4-(benzyloxy)phenyl]- 1 -cyclohexyl- 1 H-pyrazol-3-yl } -2- propenoate;
(3) (2E)-3-[l-cyclohexyl-5-(4-benzyloxyphenyl)-lH-pyrazol-3-yl]-2-propenoic acid;
(4) methyl (2Ε)-3-(5-{4-[(4'-chloro-4-methoxy-l,l'-biphenyl-2- yl)methoxy]phenyl}-l-cyclohexyl-lΗ-pyrazol-3-yl)-2-propenoate;
(5) (2E)-3-(5- { 4- [(4*-chloro-4-methoxy- 1 , 1 '-biphenyl-2-yl)methoxy]phenyl } - 1 - cyclohexyl- lH-pyrazol-3-yl)-2-propenoic acid;
(6) ethyl (2E)-3-{5-[4-(benzyloxy)phenyl]-l-cyclohexyl-lH-pyrazol-3-yl}-2- methyl-2-propenoate;
(7) (2E)-3-{5-[4-(benzyloxy)phenyl]-l-cyclohexyl-lH-pyrazol-3-yl}-2-methyl-2- propenoic acid;
(8) ethyl (2E)-3-(5-{4-[(4'-chloro-4-methoxy-l,r-biphenyl-2- yl)methoxy]phenyl } - 1 -cyclohexyl- lH-pyrazol-3-yl)-2-methyl-2-propenoate;
(9) (2E)-3-(5-{4-[(4'-chloro-4-methoxy-l,l'-biphenyl-2-yl)methoxy]phenyl}-l- cyclohexyl-lH-pyrazol-3-yl)-2-methyl-2-propenoic acid;
(10) ethyl (2E)-3-(5-{4-[(t-butyl-2-bromo-5-phenylcarboxylate)methoxy]phenyl}- 1 -cyclohexyl- lH-pyrazol-3-yl)-2-methyl-2-propenoate;
(11) ethyl (2E)-3-(5-{4-[(4"-chloro-4-t-butoxycarbonyl-l,l'-biphenyl-2- yl)methoxy]ρhenyl}-l-cyclohexyl-lH-pyrazol-3-yl)-2-methyl-2-propenoate;
( 12) ethyl (2E)-3-(5- { 4-[(4' -Chloro-4-N-methylcarbamoyl- 1 , 1 '-biphenyl-2- yl)methoxy]phenyl } - 1 -cyclohexyl- lH-pyrazol-3-yl)-2-methyl-2-propenoate; (13) (2E)-3-(5-{4-[(4'-Chloro-4-N-methylcarbamoyl-l,l'-biphenyl-2- yl)methoxy]phenyl}-l-cyclohexyl-lH-pyrazol-3-yl)-2-methyl-2-propenoic acid;
(14) methyl 3- { 5-[4-(benzyloxy)phenyl] - 1 -cyclohexyl- 1 H-pyrazol-3- yljpropanoate;
(15) 3-{5-[4-(benzyloxy)phenyl]-l-cyclohexyl-lH-pyrazol-3-yl}propanoic acid;
( 16) methyl 3-(5- { 4-[(4'-chloro-4-methoxy- 1 , 1 '-biphenyl-2-yl)methoxy]phenyl } - 1 - cyclohexyl- lH-pyrazol-3-yl)propanoate;
(17) 3-(5-{4-[(4'-chloro-4-methoxy-l,l'-biphenyl-2-yl)methoxy]phenyl}-l- cyclohexyl-lH-pyrazol-3-yl)propanoic acid;
(18) ethyl 3-{5-[4-(benzyloxy)phenyl]-l-cyclohexyl-lH-pyrazol-3-yl}-2- propynoate;
(19) 3- { 5 - [4-(benzyloxy)phenyl] - 1 -cyclohexyl- 1 H-pyrazol-3-yl } -2-propynoic acid;
(20) ethyl 3-(5- {4-[(4'-chloro-4-methoxy- 1 , 1 '-biphenyl-2-yl)methoxy]ρhenyl } -1 - cyclohexyl- lH-pyrazol-3-yl)-2-propynoate;
(21) 3-(5- { 4- [(4'-chloro-4-methoxy- 1 , 1 '-biphenyl-2-yl)methoxy]phenyl } - 1 - cyclohexyl-lH-pyrazol-3-yl)-2-propynoic acid;
(22) ethyl 5-[4-(benzyloxy)phenyl]-4-bromo-l-cyclohexyl-lH-pyrazole-3- carboxylate;
(23) methyl (2E)-3-{5-[4-(benzyloxy)phenyl]-4-bromo-l -cyclohexyl- lH-pyrazol- 3-yl } -2-propenoate; (24) (2E)-3- { 5-[4-(benzyloxy)phenyl]-4-bromo- 1 -cyclohexyl- 1 H-pyrazol-3-yl } -2- propenoic acid;
(25) methyl (2E)-3-{5-[4-(benzyloxy)phenyl]-l-cyclohexyl-4-phenyl-lH-pyrazol- 3-yl}-2-propenate;
(26) (2E)-3- { 5-[4-(benzyloxy)phenyl]- 1 -cyclohexyl -4-phenyl- 1 H-pyrazol-3-yl } -2- propenoic acid;
(27) methyl (2E)-3-(5-{4-[4'-chloro-4-methoxy-l,l-biphenyl)methoxy]phenyl}-l- cyclohexyl-4-phenyl-5-lH-pyrazol-3-yl]-2-propenoate;
(28) (2E)-3-(5- { 4- [4' -chloro-4-methoxy- 1 , 1 -biphenyl)methoxy]phenyl } - 1 - cyclohexyl-4-phenyl-5-lH-pyrazol-3-yl]-2-propenoic acid;
(29) ethyl l-[4-(benzyloxy)phenyl]-5-cyclohexyl-lH-pyrazole-3-carboxylate;
(30) methyl (2E)-3-{ l-[4-(benzyloxy)phenyl]-5-cyclohexyl-lH-pyrazol-3-yl}-2- propenoate;
(31 ) (2E)-3- { 1 -[4-(benzyloxy)phenyl]-5-cyclohexyl- 1 H-pyrazol-3-yl } -2-propenoic acid;
(32) methyl (2E)-3-(l-{4-[(4'-chloro-4-methoxy-l,l'-biphenyl-2- yl)methoxy] phenyl } -5-cyclohexyl- lH-pyrazol-3-yl)-2-propenoate;
(33) (2E)-3-( 1- { 4-[(4'-chloro-4-methoxy- 1 , 1 '-biphenyl-2-yl)methoxy]phenyl } -5- cyclohexyl-lH-pyrazol-3-yl)-2-propenoic acid;
(34) ethyl (2E)-3-{ l-[4-(benzyloxy)phenyl]-5-cyclohexyl-lH-pyrazol-3-yl}-2- methyl -2-propenoate; (35) (2E)-3-{ l-[4-(benzyloxy)phenyl]-5-cyclohexyl-lH-pyrazol-3-yl}-2-methyl-2- propenoic acid;
(36) ethyl (2E)-3-(l-{4-[(4'-chloro-4-methoxy-l,l'-biphenyl-2- yl)methoxy]phenyl}-5-cyclohexyl-lH-pyrazol-3-yl)-2-methyl-2-propenoate;
(37) (2E)-3-( 1 - { 4-[(4'-chloro-4-methoxy- 1 , 1 '-biρhenyl-2-yl)methoxy]phenyl } -5- cyclohexyl-lH-pyrazol-3-yl)-2-methyl-2-propenoic acid;
(38) ethyl (2Ε)-l-[4-(4'-Chloro-4-methylcarbamoyl-biphenyl-2-ylmethoxy)- phenyl]-5-cyclohexyl-lΗ-pyrazol-3-yl]-2-methyl-2-propenoate;
(39) (2E)-l-[4-(4'-chloro-4-methylcarbamoyl-biphenyl-2-ylmethoxy)-phenyl]-5- cyclohexyl-lH-pyrazol-3-yl]-2-methyl-2-propenoic acid;
(40) methyl 3-{ l-[4-(benzyloxy)phenyl]-5-cyclohexyl-lH-pyrazol-3- yljpropanoate;
(41) 3-{ l-[4-(benzyloxy)phenyl]-5-cyclohexyl-lH-pyrazol-3-yl}propenoic acid;
(42) methyl 3-( 1 - { 4-[(4'-chloro-4-methoxy- 1 , 1 '-biphenyl-2-yl)methoxy]phenyl } -5- cyclohexyl-lH-pyrazol-3-yl)propanoate;
(43) 3-( 1 - { 4- [(4'-chloro-4-methoxy- 1 , 1 '-biphenyl-2-yl)methoxy]phenyl } -5- cyclohexyl- lH-pyrazol-3-yl)propanoic acid;
(44) tert-butyl (2E)-3-{2-[4-(benzyloxy)phenyl]-l-cyclohexyl-lH-imidazol-4-yl}- 2-propenoate;
(45) (2E)-3-{2-[4-(benzyloxy)phenyl]-l-cyclohexyl-lH-imidazol-4-yl}-2- propenoic acid; (46) methyl (2E)-3- { 2- [4-(benzyloxy)phenyl] - 1 -cyclohexyl- lH-imidazol-4-yl } -2- propenoate;
(47) methyl (2Ε)-3-(2- { 4-[(4'-chloro-4-methoxy- 1 , 1 '-biphenyl-2- yl)methoxy]phenyl}-l-cyclohexyl-lΗ-imidazol-4-yl)-2-propenoate;
(48) (2E)-3-(2- { 4-[(4'-chloro-4-methoxy- 1 , 1 '-biphenyl-2-yl)methoxy]phenyl } - 1 - cyclohexyl- 1 H-imidazol-4-yl)-2-propenoic acid;
(49) ethyl (2E)-3-{2-[4-(benzyloxy)phenyl]-l-cyclohexyl-lH-imidazol-4-yl}-2- methyl-2-propenoate;
(50) (2Ε)-3- { 2-[4-(benzyloxy)phenyl]- 1 -cyclohexyl- lΗ-imidazol-4-yl } -2-methyl- 2-propenoic acid;
(51) ethyl (2E)-3-(2-{4-[(4'-chloro-4-methoxy-l,l'-biphenyl-2- yl)methoxy]ρhenyl }-l-cyclohexyl-lH-imidazol-4-yl)-2-methyl-2-propenoate;
(52) (2E)-3-(2- { 4-[(4'-chloro-4-methoxy- 1 , 1 '-biphenyl-2-yl)methoxy]phenyl } - 1 - cyclohexyl-lH-imidazol-4-yl)-2-methyl-2-propenoic acid;
(53) t-Butoxy (2E)-3-(2- { 4-[(4'-chloro-4-N-methylcarbamoyl- 1 , 1 '-biphenyl-2- yl)methoxy]phenyl}-l-cyclohexyl-lH-imidazol-4-yl)-2-methyl-2-propenoate; and
(54) (2E)-3-(2-{4-[(4'-chloro-4-N-methylcarbamoyl-l,l'-biphenyl-2- yl)methoxy]phenyl}-l-cyclohexyl-lH-imidazol-4-yl)-2-methyl-2-propenoic acid.
"Aryl" includes both carbocyclic and heterocyclic aromatic ring systems. "Het" means a heteroaryl ring. "Alkyl" and "alkoxy" include straight and branched confugurations. The term "halogen" includes fluorine, chlorine, bromine, and iodine.
The invention includes all pharmaceutically acceptable salt forms of the instant compounds. Pharmaceutically acceptable salts are those in which the counter ions do not contribute significantly to the physiological activity or toxicity of the compounds and as such function as pharmacological equivalents. In many instances, salts have physical properties that make them desirable, such as solubility or crystallinity. The salts can be made according to common organic techniques employing commercially available reagents. Suitable anionic salt forms include acetate, acistrate, besylate, bromide, chloride, citrate, fumarate, glucouronate, hydrobromide, hydrochloride, hydroiodide, iodide, lactate, maleate, mesylate, nitrate, pamoate, phosphate, succinate, sulfate, tartrate, tosylate, and xinofoate. Suitable cationic salt forms include ammonium, aluminum, benzathine, bismuth, calcium, choline, diethylamine, diethanolamine, lithium, magnesium, meglumine,
4-phenylcyclohexylamine, piperazine, potassium, sodium, tromethamine, and zinc.
The invention also includes all solvated forms of the instant compounds. Solvate forms do not contribute significantly to the physiological activity or toxicity of the compounds and as such function as pharmacological equivalents. Solvates may form in stoichiometric amounts or may form with adventitious solvent or a combination of both. One type of solvate is hydrate and some hydrated forms include monohydrate, hemihydrate, and dihydrate, but other forms can be encountered.
Synthesis
The novel compounds of this invention can be prepared using reactions and techniques described in this section or known in the art. The starting materials are commercially available or can be prepared by methods known in the art. Reactions are performed in solvents appropriate to the reagents and materials employed.
Synthetic routes to carboxylate acids and esters depicted below can be adapted by methods known to one skilled in the art to allow for the preparation of nitriles, halogens, or sulphonate esters, or other functional groups that may be converted to carboxylate isosteres by various methods. These substituents may be interconverted as necessary by a wide variety of methods known to one skilled in the art of organic synthesis. Scheme 1 describes the synthesis of 1-cycloalkylpyrazoles and begins with the conversion of protected 4-hydroxyphenyl methyl ketones (1.1) to diketoesters 1.2. This intermediate can be condensed with a cycloalkyl-substituted hydrazines to afford pyrazoles 1.3. Thus, substituents R1 and R2 and R3 can be varied by the choice of starting materials, and if R is initially H, then it can be replaced by bromine via a bromination reaction, and further modified via Pd° coupling or metal-halogen exchange followed by reaction with an appropriate electrophile.
Scheme 1
Figure imgf000015_0001
Scheme 2 depicts elaboration to α,β-unsaturated carboxylates beginning from esters 1.3 of Scheme 1. Reduction to alcohols 2.1 followed by oxidation can give aldehydes 2.2 which can then be coupled with an appropriate Wittig or equivalent reagent to provide the α,β-unsaturated esters 2.3. Saponification of 2.3 can provide carboxylic acids 2.4. It is envisioned that aldehydes 2.2 can be coupled with olefination reagents to provide α,β -unsaturated sulfonate esters, nitriles, or other intermediates that can be converted to carboxylate isosteres. Scheme 2
Figure imgf000015_0002
Preparation of biphenyl sidechain analogs as drawn in Scheme 3 requires removal of R1 from 2.3 to liberate phenols 3.1. Alkylation 3.1 can give biphenyl analogs 3.2 which can be hydrolyzed at the methyl ester to provide compounds 3.3. Substituents R4 and R5 can be varied by choice of reagent.
Scheme 3
Figure imgf000016_0001
3.1
Figure imgf000016_0002
In Scheme 4, reaction of aldehyde analogs 2.2 with a Wittig like reagent can give methyl substituted analogs of structure 4.1, and hydroylsis of 4.1 can give carboxylic acids 4.2. Alternatively, deprotection (removal of R from 4.1) can give phenols 4.3, and alkylation of the phenols can provide biphenyl analogs 4.4 which can be further subjected to ester saponification to arrive at carboxylic acids 4.5 possessing HCV inhibitory activity.
Scheme 4
Figure imgf000016_0003
As outlined in Scheme 5, compounds of structure 4.3 can be alkylated to give bromide analogs 5.1. Theses analogs can be derivatized via coupling chemistry to biphenyl structures 5.2. Cleavage of the tert-butyl ester can give carboxylates 5.3 which can be coupled with amines to form amides 5.4. Saponification of the ethyl ester can give carboxylates 5.5.
Scheme 5
Figure imgf000017_0001
5 1 5.2 \
Figure imgf000017_0002
55 5.4 5.3
The olefinic double can be reduced to give saturated compounds of scheme 6. Hydrogenation of methyl esters 2.3 can provide propanoates 6.1, and propanoic acids 6.2 after saponification. Following the sequence of Scheme 3, phenols 6.3 can be obtained upon deprotection of 6.1, and alkylation of 6.3 as above can give biphenyl analogs 6.4. A further step of hyrolysis can give carboxylic acid compounds 6.5.
Scheme 6
Figure imgf000017_0003
Scheme 7 depicts the synthetic entry into an alkynyl series of biphenyl analogs. The reaction of carbon tetrabromide and triphenylphosphene on aldehyde 2.2 can give vinyl dibromides 7.1, which upon subjection to butyl lithium can afford acetylenes 7.2. Deprotonation and quench with chloroformate can provide ethyl esters 7.3 which can be hydrolyzed to 7.4. Alternatively, deprotection of 7.3 yields phenols 7.5 which can be further subjected to alkylation, and the resulting biphenyl analogs 7.6 can be saponified to carboxylic acid compounds 7.7.
Scheme 7
Figure imgf000018_0001
Scheme 8 outlines bromination of pyrazoles 1.3 to give bromides 8.1 which can be used to obtain pyrazoles substituted at the 4-postion. Thus, 8.1 can be reduced to alcohols 8.2, oxidized to aldehydes 8.3, treated with Wittig reagent to afford propenoates 8.4, and saponified to 8.5 according to methods above. Bromides 8.4 can be coupled with phenyl boronic acids to give rise to 4-phenyl analogs 8.6, saponification of which leads to acids 8.7, or removal of R1 can give phenols 8.8. As above, alkylation of the phenol can give biphenyls 8.9, and saponification can provide carboxylates 8.10.
Figure imgf000019_0001
8 7
Scheme 9 depicts preparation of another type of compound, 5- cycloalkylpyrazole derivatives. Diketoesters 9.1 can be obtained upon treatment of cycloalkyl methyl ketones with ethyl oxalate, and can be subsequently condensed with substituted phenylhydrazines to give 5-cycloalkylpyrazoles 9.2. Substituents R , R , and R can be varied by the choice of starting materials. If substituent R is H, then bromine can be introduced via bromination with Br2 in acetic acid, followed by further modification of the bromo substituent via Pd° coupling or other derivation known to those skilled in the art. As above, reduction of 9.2 to alcohols 9.3, oxidation of 9.3 to aldehydes 9.4, and Wittig reaction to 9.5 can give compounds suitable for further elaboration to biphenyl derivatives. While saponification of esters 9.5 can give acids 9.6, deprotection of 9.5 (removal of R3) can provide 9.7 which can be alkylated to biphenyls 9.8, and further saponification can yield carboxylates 9.9.
Scheme 9
Figure imgf000020_0001
Figure imgf000020_0002
94 93
Figure imgf000020_0003
98
In a simlar sequence to Schemes 4 and 5, aldehydes 9.4 can be carried forward (Scheme 10) to give biphenylamides. Thus, exposure to Wittig conditions can afford 2-methylpropenaotes 10.1 which can be saponified to acids 10.2. Alternatively, 10.1 can be deprotected (removal of R3) to generate phenols 10.3 which can be alkylated to give biphenyl amides 10.4, and further saponification provides carboxylic acids 10.5.
Scheme 10
Figure imgf000021_0001
In similar manner to Scheme 6, propenoates 9.5 can be reduced to give saturated compounds 11.1 shown in Scheme 11. Saponification can provide carboxylic acids 11.2, and removal of R3 gives phenols 11.3. Alkylation to biphenyl derivatives 11.4 and hydrolysis of 11.4 provides propanoic acids analogs 11.5.
Scheme 11
Figure imgf000021_0002
11 2
Figure imgf000021_0003
11 4 11 5 Cycloalkylimidazole derivatives are depicted in scheme 12. Cycloalkylimdazoles 12.1 can be brominated to tribromide 12.2. Coupling at the 2- position can give 12.3 which can be selectively debrominated to 12.4, and coupled at the 4-position to give 12.5 which can be saponified to generate carboxylates 12.6. Alternatively, transmetalation of 12.4 and quench with DMF can give aldehydes 12.7. Wittig olefination of 12.7 can afford α,β-unsaturated esters 12.8 which can be deprotected (removal of R2) to yield phenols 12.9. Alkylation of 12.9 and saponification of the resulting biphenyl analogs can give 12.10 and 12.11, respectively.
Scheme 12 o " -N "-S-N OR' R1
Figure imgf000022_0001
Figure imgf000022_0002
As depicted above in schemes 4 and 10, a similar sequence is defined in scheme 13 for 2-methyl substitution on the propenoate sidechain. Thus, aldehydes 12.7 can be treated with Wittig-like reagents to provide derivatives 13.1, which can be saponified to 13.2, or deprotected to phenols 13.3. Alklation of phenols can give biphenyls 13.4, and saponification can give carboxylates 13.5. Scheme 13
Figure imgf000023_0001
Carboxylate isostere groups can be incorporated into the compounds of this invention. The heterocycles prepared in schemes 1-13 each bear a carboxylic acid liberated through hydrolysis in the final step of the synthesis. The carboxylic acid can be replaced with an equivalent group or isostere introduced in the final step of synthesis, or during synthesis of precursors to the final compound. In schemes 14- 19, only the carboxylate isostere and its attached carbon atom are explicitly shown, however, they can be incorporated into the heterocycles of this invention by methods known in the art of synthetic organic chemistry.
Scheme 14 illustrates the synthesis of tetrazole derivatives. For a leading reference, see: Duncia, J. V., et al. J. Org. Chem. (1991), 56, 2395-2400; Herr, J. R. Bioorg. & Med. Chem. (2002), 10, 3379-3393. Nitrile 14.1 can be reacted with a trialkyltin azide, optionally prepared in situ from a trialykltin chloride and metal azide, in a solvent such as toluene at elevated temperature to afford tetrazole 14.2. The trialkyltin moiety can be removed by treatment with hydrogen chloride to afford the deprotected tetrazole 14.3. As an alternative, nitrile 14.1 can be converted directly to tetrazole 14.3 by treatment with sodium azide and ammonium chloride (or trialkylammonium hydrochloride, in an aromatic solvent: Koguro, K. et al. Synthesis (1998), 910-914) in a solvent such as DMF at elevated temperature. Another approach, where Rl is a carboxylic acid, the acid 14.4 can be coupled to 3- aminopropionitrile using DCC in dichloromethane. Treatment of the resulting amide 14.5 with sodium azide under Mitsonobu conditions can afford the tetrazole 14.6, which may be deprotected by a variety of basic reagents to provide the deprotected tetrazole 14.3.
Scheme 14 N3SnR3 N' |j HCl " N' R3Sn T H ~~ 14 1 142 14 3
Figure imgf000024_0001
Scheme 15 depicts the synthesis of acylsulfonamide derivatives from carboxylic acids and primary carboxamides. Carboxylic acid 14.4 can be treated with a sulfonamide and a coupling agent, l-(3-dimethylaminoproρyl-3-ethylcarbodiimide (EDC), and DMAP (Sturino, C. F., et al. Tetrahedron Lett. (1998), 39, 5891-5894) to generate acylsulfonamide 15.1. Other conditions include formation of a carbonylimidazolide by treatment of 14.4 with carbonyl diimidazole (CDI), followed by a sulfonamide and DBU (Drummond, J. T.; Johnson, G. Tetrahedron Lett. (1988), 27, 1653-1656), or formation of an acid chloride by treatment of 14.4 with thionyl chloride, followed by reaction with the sodium salt of a sulfonamide in DMF/dichloromethane. Alternatively, compound 15.1 can be prepared by reaction of primary amide 15.2 with an appropriate sulfonyl chloride in a solvent such as pyridine at elevated temperature (Cossu, S.; Giacomelli, G.; Conti, S.; Falorni, M.; Tetrahedron (1994), 50, 5083-5090).
Scheme 15
Figure imgf000025_0001
14.4 15 1
O H H?2NN ■ V - a-''3^CI 15.1 15 2
The synthesis of acylsulfonamide compounds from primary sulfonamides or sulfonic acids is shown in scheme 16 where compound 16.1 can be converted to an acylsulfonamide 16.2 upon reaction with an acid chloride and DMAP in pyridine or with an isocyanate (Mantlo, N. B. et al. Bioorg. Med. Chem. Lett. (1994), 4, 17-22). Moreover, sulfonic acid 16.3 can be converted to sulfonyl chloride 16.4 by treatment with phosphorous oxychloride, phosphorous pentachloride, or a mixture of the two, or with thionyl chloride, in a solvent such as benzene, toluene, or DMF. Addition of a primary amide to chloride 16.4 is facilitated by DMAP in pyridine at elevated temperature to provide another entry into acylsulfonamides 16.2.
Scheme 16
Figure imgf000025_0002
16 1 16 2
Figure imgf000025_0003
16 3 16 4 Hydroxylamine can be added to nitrile 14.1 to form amidoxime 17.1 in solvents such as EtOH, DMF, or DMSO at elevated temperature as outlined in scheme 17. Amidoxime 17.1 can be further cyclized to 5-oxo-l,2,4-oxadiazole 17.2 when treated with methylchloroformate and a base reagent such as pyridine, followed by reflux in toluene (Kohara, Y. et al. Bioorg. Med. Chem. Lett. (1995), 5, 1903- 1908). Alternatively, 17.1 can form oxathiadiazole 17.3 in the presence of SOCl2 in pyridine (Kim, D. et al. Bioorg. Med. Chem. Lett. (1994), 4, 41-44). And, in a similar way amidoxime 17.1 can be converted to oxadiazole 17.4 when reacted with thiocarbonyldiimidazole and DBU in acetonitrile (Gezginci, et al. J. Med. Chem. (2001), 44, 1560-1563).
Scheme 17
Figure imgf000026_0001
14.1 17.1 17.2
Figure imgf000026_0002
17.3
Figure imgf000026_0003
17.4 Scheme 18 depicts synthesis of 3-hydroxy-3-cyclobutene-l,2-dione compounds from iodide 18.1 which can be coupled to stannyl cyclobutene 18.2 (Soil, R. M. et al. Bioorg. Med. Chem. Lett. (1993), 3, 757-760) using catalytic trans-benzyl(chloro)bis(triphenyl phosphine)palladium(II) and copper(I) iodide in acetonitrile at elevated temperature (Liebskind, L. S.; Fengl, R. W. J. Org. Chem. (1990), 55, 5359) to afford compound 18.3, and acidic hydrolysis can provide 3- hydroxy-3-cyclobutene- 1 ,2-dione 18.4. Scheme 18
Figure imgf000027_0001
18.1 18.3 18.4
Carboxylic acid 14.4 can be coupled with 5-aminornethyltetrazole 19.1 (available commercially from Dynamit Nobel GmbH, Leverkusen, Germany) using any of a variety of peptide coupling reagents, including EDC and 1- hydroxybenzotriazole in dichloromethane or DMF, to give lH-tetraazol-5-ylmethyl carboxamide 19.2. Under similar reaction conditions, 14.4 can be coupled with 5- aminotetrazole 19.3 to afford lH-tetraazol-5-yl carboxamide 19.4.
Scheme 19
Figure imgf000027_0002
14.4 19.2 NH, N N O H N 14.4 19.3 N H 19.4
Other carboxylate isosteres claimed in the present invention can be synthesized by methods known to one skilled in the art of organic synthesis. For example, preparation of phosphinic and phosphonic acid analogs (Kehler, J. et al. Bioorg. Med. Chem. Lett. (2000), 10, 2547-2548), hydroxamic acids (Golebiowski Tet. Lett (1998), 39, 3397), etc. Biological Activity
Compounds of Formula I inhibit the activity of Hepatitis C Virus NS5B RdRp as demonstrated using assays measuring NS5B RdRp activity.
HCV NS5B RdRp cloning, expression, and purification. The cDNA encoding the NS5B protein of HCV, genotype lb, was cloned into the pET21a expression vector. The protein was expressed with an 18 amino acid C-terminal truncation to enhance the solubility. The E. coli competent cell line BL21(DE3) was used for expression of the protein. Cultures were grown at 37°C for ~ 4 hours until the cultures reached an optical density of 2.0 at 600 nm. The cultures were cooled to 20°C and induced with 1 mM EPTG. Fresh ampicillin was added to a final concentration of 50 ug/ml and the cells were grown overnight at 20°C. Cell pellets (3L) were lysed for purification to yield 15-24 mgs of purified
NS5B. The lysis buffer consisted of 20 mM Tris-HCl, pH 7.4, 500 mM NaCl, 0.5% triton X-100, 1 mM DTT, ImM EDTA, 20% glycerol, 0.5 mg/ml lysozyme, 10 mM MgC12, 15 ug/ml deoxyribonuclease I, and Complete TM protease inhibitor tablets (Roche). After addition of the lysis buffer, frozen cell pellets were resuspended using a tissue homogenizer. To reduce the viscosity of the sample, aliquots of the lysate were sonicated on ice using a microtip attached to a Branson sonicator. The sonicated lysate was centrifuged at 100,000 x g for lhr at 4°C and filtered through a 0.2 μ filter unit (Corning). The protein was purified using three sequential chromatography steps:
Heparin sepharose CL-6B, polyU sepharose 4B, and Hitrap SP sepharose (Pharmacia). The chromatography buffers were identical to the lysis buffer but contained no lysozyme, deoxyribonuclease I, MgC12 or protease inhibitor and the NaCl concentration of the buffer was adjusted according to the requirements for charging the protein onto the column. Each column was eluted with a NaCl gradient which varied in length from 5-50 column volumes depending on the column type. After the final chromatography step, the resulting purity of the enzyme is >90% based on SDS-PAGE analysis. The enzyme was aliquoted and stored at -80°C. HCV NS5B RdRp enzyme assay. HCV RdRp genotype lb assays were run in assay buffer composed of 20 mM Tris-HCl, pH 7.5, 2.5 mM KC1, 5 mM MgCl2, 1 mM DTT, 1.6 U RNAse inhibitor (Promega N2515), in 96 well plates (Falcon 3918). All compounds were serially diluted in DMSO and diluted further in assay buffer such that the final concentration of DMSO in the assay was 2%. Compounds were serially diluted (3-fold each time) for a 7 point inhibition analysis. HCV RdRp genotype lb enzyme was used at a final concentration of 28 nM. A polyA template was used at 28 nM, and the oligo-dT12-18 primer was used at 840 nM final concentration. Preannealed primer and template were obtained commercially (Amersham 27-787802). 3H-UTP was used at 0.125 μCi (1 μM total UTP). Reaction was initiated by the addition of enzyme. Reactions were incubated at 30°C for 45 min, and stopped by adding 30 ul of 20% ice cold TCA. Plates were chilled for 30 minutes and harvested onto Unifιlter-96, GF/B plates(Packard, 6005177) using a Packard FilterMate Cell Harvester. The harvest plates were prewashed 3 times, 200ul/well, with 100 mM NaPPi. Harvested filters were washed 30 times, 200 ul/well, with distilled water followed by ethanol. Filter plates were dried, and 30 ul /well microscint-20 was added. Plates were read on a Packard Top Count NXT.
The IC50 values for compounds were determined using six different [I], combined with 7nM enzyme, 800 ng of the template-primer polyC/oligoG1 (1:5 molar ratio), and 0.7 uM of 3H GTP containing 1 uCi. The observed fractional activity (fa = vi/vo) was used in the equation IC50 = [I]/(l/fa-l) to determine a single point IC50 value. Typically, the single point IC50 values derived from [I] that produced fractional activities in the range of 0.1 to 0.8 relative to the uninhibited control were averaged to calculate the IC50 value for each compound.
FRET Assay Preparation. To perform the HCV FRET screening assay, 96- well cell culture plates were used. The FRET peptide (Anaspec, Inc.) (Taliani et al., Anal. Biochem. 240:60-67 (1996), expressly incorporated by reference in its entirety) contains a fluorescence donor, EDANS, near one end of the peptide and an acceptor, DABCYL, near the other end. The fluorescence of the peptide is quenched by intermolecular resonance energy transfer (RET) between the donor and the acceptor, but as the NS3 protease cleaves the peptide the products are released from RET quenching and the fluorescence of the donor becomes apparent.
The assay reagent was made as follows: 5X cell Luciferase cell culture lysis reagent from Promega (#E153A) diluted to IX with dH O, NaCl added to 150 mM final, the FRET peptide diluted to 20 uM final from a 2 mM stock. Cells were trypsinized, placed into each well of a 96-well plate and allowed to attach overnight. The next day, the test compounds were added to columns 1 through 10; column 11 was media only, and column 12 contained a titration of interferon as a control (lOOOunits for A12, B12, lOOunits for C12, D12, lOunits for E12, F12 and lunit for G12, H12). The plates were then placed back in the incubator.
FRET Assay and Cytotoxicity Assay. Subsequent to addition of the test compounds described above (FRET Assay Preparation), at various times the plate was removed and Alamar blue solution (Trek Diagnostics, #00-100) was added per well as a measure of cellular toxicity. After reading in a Cytoflour 4000 instrument (PE Biosystems), plates were rinsed with PBS and then used for FRET assay by the addition of 30 ul of the FRET peptide assay reagent described above (FRET Assay Preparation) per well. The plate was then placed into the Cytoflour 4000 instrument which had been set to 340 excite/490 emission, automatic mode for 20 cycles and the plate read in a kinetic mode. Typically, the signal to noise using an endpoint analysis after the reads was at least three-fold.
Compound analysis was determined by quantification of the relative HCV replicon inhibition and the relative cytotoxicity values. To calculate cytoxicity values, the average Alamar Blue fluorescence signals from the control wells in row 11 were set as 100% non-toxic. The individual signals in each of the compound test wells were then divided by the average control signal and multiplied by 100% to determine percent cytotoxicity. To calculate the HCV replicon inhibition values, an average background value FRET signal was obtained from the two wells containing the highest amount of interferon at the end of the assay period. These numbers were similar to those obtained from naϊve Huh-7 cells. The background numbers were then subtracted from the average FRET signal obtained from the control wells in row 11 and this number was used as 100% activity. The individual signals in each of the compound test wells were then divided by the averaged control values after background subtraction and multiplied by 100% to determine percent activity. EC50 values for an interferon titration were calculated as the concentration which caused a 50% reduction in HCV RNA, HCV protein amounts or FRET activity. The two numbers generated for the compound plate, percent cytoxicity and percent activity were used to determine compounds of interest for further analysis.
The HCV NS5B RdRp enzyme inhibition assay results are tabulated in Table
1.
Table 1.
Figure imgf000031_0001
Figure imgf000032_0001
Pharmaceutical Compositions and Methods of Use
The compounds of this invention inhibit HCV NS5B RNA-dependent RNA polymerase (RdRp). This polymerase is one of a small number of functional enzymes encoded by the viral RNA and is known to be essential for infectivity of chimpanzees. By inhibiting this enzyme, the compounds of this invention are useful for impeding or preventing HCV infection and for treating hepatitis C. At least one other compound which acts on this enzyme is currently undergoing clinical evaluation for hepatitis C (Tan, S.-L.; Pause, A.; Shi, Y.; Sonenberg, N. Nature Reviews/Drug Discovery 2002, 1, 867-881).
The compounds of this invention are generally given as pharmaceutical compositions comprised of a therapeutically effective amount of a compound of Formula I or its pharmaceutically acceptable salt and a pharmaceutically acceptable carrier and may contain conventional exipients. A therapeutically effective amount is that which is needed to provide a meaningful patient benefit. Pharmaceutically acceptable carriers are those conventionally known carriers having acceptable safety profiles. Compositions encompass all common solid and liquid forms including capsules, tablets, losenges, and powders as well as liquid suspensions, syrups, elixers, and solutions. Compositions are made using common formulation techniques, and conventional excipients (such as binding and wetting agents) and vehicles (such as water and alcohols) are generally used for compositions. Compositions are normally formulated in dosage units and compositions providing from about 1 to 1000 mg of the active ingredient per dose are preferred.
Methods of treatment involve administering a therapeutically effective amount of a Formula I compound or a pharmaceutically acceptable salt to a patient exhibiting conditions responsive to HCV NS5B RdRp inhibition. The methods include all conventional modes of administration. Typical modes are oral, topical, rectal, nasal, and parenteral. Generally, the daily dosage will be from about 0.001 mg to 100 mg of Formula I compound per kilogram of body weight when used for hepatitis C. The specific dosing regimen, however, must be carefully adjusted using sound medical j udgement.
The compounds of this invention can be used with other agents that inhibit HCV viral replication including replicase inhibitors, metalloprotease inhibitors, NS3 protease inhibitors, NS3 helicase inhibitors, NS5A inhibitors-including interferons (IFN), PEGylated interferons, and ribavirin-and NS5B polymerase inhibitors.
Additionally, the compounds can be used in conjunction with other hepatitis C compounds including inosine monophophate dehydrogenase (IMPDH) inhibitors, immune modulators, serine protease inhibitors, immunoglobulin immunosuppressants, antivirals, antifibrotics, caspase inhibitors, and tubulin inhibitors, as well as monoclonal antibodies, ribozymes, and antisense agents.
The table below lists some compositions that can be administered with the compounds of this invention. The compounds of this invention can be administered with these compositions in combination therapy -either jointly or separately-or by combining the compounds with one or more of the compositions into a new composition. Table 2.
Figure imgf000034_0001
Figure imgf000035_0001
DESCRIPTION OF SPECIFIC EMBODIMENTS
Abbreviations
Solution ratio express a volume relationship, unless stated otherwise. NMR chemical shifts (δ) are reported in parts per million. Flash chromatography was carried out on silica gel according to Still's method (Still, W. C. et al. J. Org. Chem. (1978), 43, 2923). Abbreviations used in the Examples are defined as follows: "°C" for degrees Celsius, "MS" for mass spectrometry, "ESI" for electrospray ionization mass spectroscopy, "HR" for high resolution, "LC-MS" for liquid chromatography mass spectrometry, "eq" for equivalent or equivalents, "g" for gram or grams, "h" for hour or hours, "mg" for milligram or milligrams, "ml" for milliliter or milliliters, "mmol" for millimolar, "M" for molar, "min" for minute or minutes, "HPLC" for high pressure liquid chromatography, "rt" for room temperature, "NMR" for nuclear magnetic resonance spectroscopy, "tic" for thin layer chromatography, "atm" for atmosphere, and "α", "β", "R", "S", "E", and "Z" are stereochemical designations familiar to one skilled in the art. As used throughout the specification, the following abbreviations for chemical reagents apply: Boc is tert-butyl oxycarbonyl, BuLi is n-butyl lithium Cbz is carbonylbenzyloxy, DCE is 1,2-dichloroethane, DIEA is diethylpropyl amine, DMAP is dimethylaminopyridine, DME is dimethylethyleneglycol DMF is dimethylformamide, EDCI is l-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, HOAt is l-hydroxy-7-azabenzotriazole, LiHMDS is bis(trimethylsilyl)amide, NBS is N-bromosuccinamide, NCS is N-chlorosuccinamide, TBAI is tetrabutylammonium iodide, TEA is triethylamine, TFA is trifluoroacetic acid, THF is tetrahydrofuran.
Intermediate 1.2
Figure imgf000036_0001
Ethyl (2Z)-2-hydroxy-4-oxo-4-(4-benzyloxyphenyI)-2-butenoate (1.2). To a solution of 4-benzyloxyacetophenone 1.1 (20.0 g, 88.4 mmol) in DMF (100 ml) at rt, was added NaH (60% in oil, 4.26 g, 106 mmol). The mixture was stirred at rt for 45 min, cooled to 0°C, and diethyl oxalate (14.4 ml, 106 mmol) was added dropwise, and the suspension warmed to rt and stirred for 17 h. The reaction mixture was poured over a mixture of ice and IN HCl and the suspension stirred for 30 min, filtered, and the solid rinsed with water and dried to afford 28.9 g of the butenoate 1.2. MS (ESI) 325.2 (MH+). Example 1
Figure imgf000037_0001
Ethyl l-cyclohexyl-5-(4-benzyloxyphenyl)-lH-pyrazole-3-carboxylate
(1.3). The butenoate 1.2 (5.00 g, 15.3 mmol) and cyclohexylhydrazine hydrochloride (2.77 g, 18.4 mmol) were heated at refluxed in EtOH (60 ml) for 2h. Concentration in vacuo resulted in formation of a solid which was suspended in EtOAc and filtered through a 1" pad of SiO2, elution with ethyl acetate. The filtrate was concentrated to afford 6.10 g (98%) of the desired pyrazole ester 1.3. MS (ESI) 405.4 (M + H+).
Intermediate 2.1
Figure imgf000037_0002
[l-cyclohexyl-5-(4-benzyloxyphenyl)-lH-pyrazol-3-yl]methanol (2.1). To a suspension of LAH (52 mg, 1.36 mmol) in THF (6 ml) at 0°C, was added dropwise a solution of pyrazole ester 1.3 (500 mg, 1.24 mmol) in THF (3 ml). The mixture was stirred at 0°C for 30 min, quenched with H2O, diluted with IN HCl, and extracted with EtOAc (3X). The combined organic phase was washed with H2O, brine, and dried (Na2SO4). Filtration through a 1" pad of SiO2, elution with ethyl acetate, and concentration of the filtrate afforded 448 mg (100%) alcohol 2.1. HRMS (ESI) calc'd for C23H27N2O2 363.1994; found 363.2083 (MH+). Intermediate 2.2
Figure imgf000038_0001
l-cyclohexyl-5-(4-benzyloxyphenyl)-lH-pyrazole-3-carbaldehyde (2.2).
To a solution of alcohol 2.1 (400 mg, 1.10 mmol) in DME (10 ml) at reflux, was added MnO2 (880 mg), portion-wise over 4 h. After being cooled to rt, the solution was concentrated and the crude product filtered though a 1" pad of SiO2, elution with ethyl acetate. The filtrate was concentrated to give aldehyde 2.2 260 mg (91%). HRMS (ESI) calc'd for C23H25N2O2 361.1838; found 361.1926 (MH+).
Example 2
Figure imgf000038_0002
Methyl (2E)-3-{5-[4-(benzyloxy)phenyI]-l-cyclohexyl-lH-pyrazoI-3-yl}-2- propenoate (2.3). A mixture of aldehyde 2.2 (311 mg, 0.863 mmol) and methyl (triphenylphosphoranyl-idene)acetate (317 mg, 0.949 mmol) in benzene (5 ml) were heated at reflux for 6 h. Additional methyl (triphenylphosphoranylidene)acetate (100 mg) was added and the mixture and after an addition 18 h at reflux, the reaction mixture was concentrated to give a residue purified by SiO2 chromatography (stepwise gradient, 10 to 15% EtOAc/hexanes) to afford 2.3, 300 mg (83%). MS (ESI) 417.4 (M + H+). Example 3
Figure imgf000039_0001
(2E)-3- [ 1 -cyclohexyl-5-(4-benzyIoxyphenyl) - lH-py razol-3-yl] -2-propenoic acid (2.4). To a solution of ester 2.3 (55 mg, 0.132 mmol) in 4.5 ml of THF/MeOH (8:1) was added IN LiOH (2 ml), and the mixture was stirred for 24 h at rt. Concentration to remove solvent gave a residue that was taken Et2O and extracted with 0.1 N NaOH and H2O (2X). The combined aqueous extracts were acidified with concentrated HCl and extracted with EtOAc (3X). The combined organic extracts were washed with H2O, brine, dried (Na2SO4). The crude product was triturated with hexanes to afford 45.8 mg (86%) of acid 2.4. HRMS (ESI) calc'd for C25H27N2O3 403.1943; found 403.2019 (MH+); 1H NMR (300 MHz, CDC13) δ 7.83 (d, 7 = 16.1, IH), 7.49-7.34 (m, 5H), 7.28 (d, J = 8.8, 2H), 7.07 (d, = 8.8, 2H), 6.46 (s, IH), 6.38 (d, 1 = 15.7, IH), 5.13 (s, 2H), 4.14-4.04 (m, IH), 2.03-1.82 (m, 6H), 1.28-1.24 (m, 4H).
Intermediate 3.1
Figure imgf000039_0002
Methyl (2E)-3-[l-cyclohexyl-5-(4-hydroxyphenyl)-lH-pyrazol-3-yl]-2- propenoate (3.1). To a solution of ester 2.3 (50 mg, 0.120 mmol) in CH2C12 (2 ml) at -50°C was added a 1M solution of BC13 in CH2C12 (600 μl 0.60 mmol), and the mixture was stirred for 30 min at that temperature before being quenched with
MeOH, diluted with EtOAc, washed with H2O, saturated NaHCO3 (2X), brine, and dried (Na SO4). After concentration, the residue was combined with another 0.024 mmol batch for purification by SiO2 chromatography (30% EtOAc/hexanes) to afford 48 mg (100%) of phenol 3.1. MS (ESI) 327.3 (MH+), 325.3 (MK).
Example 4
Figure imgf000040_0001
Methyl (2£)-3-(5-{4-[(4,-chloro-4-methoxy-l,l,-biphenyl-2- yl)methoxy]phenyl}-l-cyclohexyl-lH-pyrazol-3-yl)-2-propenoate (3.2). A mixture of phenol 3.1 (34 mg, 0.104 mmol), 4'-chloro-4-methoxy-l,l'-biphenyl-2- yl)methyl bromide (39.0 mg, 0.125 mmol), and powdered K2CO3 (28.7 mg, 0.208 mmol) were heated at refluxed for 1.5 h in CH3CN (3 ml). The mixture was poured into EtOAc, washed with water, brine, and dried (Na2SO ). Purification by SiO chromatography (stepwise gradient: 15 to 20% EtOAc/hexanes) gave 3.2, 52 mg (93%). MS (ESI) 557.3 (MH+).
Example 5
Figure imgf000040_0002
(2E)-3-(5-{4-[(4'-chloro-4-methoxy-l,l'-biρhenyl-2-yl)methoxy]phenyl}-l- cyclohexyl-lH-pyrazol-3-yl)-2-propenoic acid (3.3). Methyl ester 3.2 (40 mg) and IN LiOH (1 ml) in 3 ml MeOH/THF (1:2) was stirred at rt 18 h before being concentrated and partitioned between EtOAc and H O. The aqueous layer was acidified with IN HCl and extracted with EtOAc (3X). The organic extracts were washed with H2O, brine, and dried (Na SO4). Concentration gave 3.3, 39 mg (100%). HRMS (ESI) calc'd for C32H32ClN2O4542.1972; found 543.2050 (MH+); 1H NMR (300 MHz, CDC13) δ 7.83 (d, / = 15.8, IH), 7.39-7.23 (m, 7H), 7.18 (d, J ■■ 2.6, IH), 6.99-6.94 (m, 3H), 6.45 (s, IH), 6.38 (d, / = 6.1, IH), 4.94 (s, 2H), 4.13- 4.01 (m, IH), 3.88 (s, 3H), 2.04-1.83 (m, 6H), 1.32-1.21 (m, 4H).
Example 6
Figure imgf000041_0001
Ethyl (2E)-3-{5-[4-(benzyloxy)phenyI]-l-cydohexyl-lH-pyrazoI-3-yI}-2- methyI-2-propenoate (4.1). BuLi (1.6 M in hexanes, 956 μL, 1.53 mmol) was added dropwise to (l-ethoxycarbonyl-thyl)triphenylphosphonium bromide (738 mg, 1.66 mmol) in THF (7 ml) at 0°C, follow by addition of aldehyde 2.2 (500 mg, 1.39 mmol as solution in 3 ml of THF). The mixture was stirred at 0°C for 30 min and warmed to rt, and after 1.5 h, H2O was added. The mixture was diluted with EtOAc, and the organic phase was washed with H2O, brine, dried (Na2SO4). After being concentrated, the resultant residue was purified by SiO2 chromatography (gradient elution: 10 to 15% EtOAc/hexanes) to provide 530 mg (86%) of 4.1. MS (ESI) 445.4 (MH+).
Example 7
Figure imgf000041_0002
(2E)-3-{5-[4-(benzyIoxy)phenyl]-l-cyclohexyI-lH-pyrazol-3-yl}-2-methyl-
2-propenoic acid (4.2). A mixture of methyl ester 4.1 (50 mg, 0.112 mmol) and IN LiOH (2 ml) in 3 ml of MeOH/THF (1:2) was stirred at rt for 18 h, concentrated, and partitioned between Et2O and H2O. The organic phase was extracted with 0.1 N NaOH (2X), and the combined aqueous extracts were acidified with cone. HCl, and extracted with EtOAc (3X). The combined organic extracts were washed with H2O, brine, and dried (Na SO4) before being concentrated to afford 47 mg (100%) of 4.2. HRMS (ESI) calc'd for C26H 9N2O3 417.2178, found 417.2174 (MH+); 1H NMR (300 MHz, CDC13) δ 7.81 (d, J = 1.1, IH), 7.49-7.36 (m, 5H), 7.30 (d, J = 8.7, 2H), 7.07 (d, 7 = 8.7, 2H), 6.45 (s, IH), 5.14 (s, 2H), 4.14-4.07 (m, IH), 2.26 (d, 7 = 1.5, 3H), 2.07-2.00 (m, 2H), 1.95-1.84 (m, 4H), 1.36-1.22 (m, 4H).
Intermediate 4.3
Figure imgf000042_0001
Ethyl (2£)-3-[l-cyclohexyl-5-(4-hydroxyphenyl)-lH-pyrazol-3-yl]-2- methyl-2-propenoate (4.3). To a solution 4.1 (83 mg, 0.187 mmol) in CH2C12 (2 ml) at -50°C was added a 1M solution of BC13 in CH2C12 (933 μL, 0.933 mmol) followed by stirring at -50°C for 15 min, and quenched with MeOH. The mixture was diluted with EtOAc, washed with H2O, sat. NaHCO3, brine, and dried (Na2SO4). Filtration through a 1" pad of SiO2 and concentration to provided 4.3, 67 mg (100%). MS (ESI) 355.3 (MH+), 353.3 (MK).
Example 8
Figure imgf000042_0002
Ethyl (2E)-3-(5-{4-[(4,-chloro-4-methoxy-l,l*-biphenyl-2- yl)methoxy]phenyl}-l-cyclohexyl-lH-pyrazol-3-yl)-2-methyl-2-propenoate (4.4).
A mixture of ethyl ester 4.3 (40.8 mg, 0.114 mmol), 4'-chloro-4-methoxy-l,l'- biphenyl-2-yl)methyl bromide (42.7 mg, 0.137 mmol), and powdered K2CO3 (31.5 mg, 0.228 mmol) were taken up in CH3CN (2 ml) and heated at reflux for 2 h prior to being poured into EtOAc, washed with water, brine, and dried (Na2SO ). Concentration and purification by SiO2 chromatography (stepwise gradient: 5 to 7.5 to 10 to 12.5% EtOAc/hexanes) to gave 67 mg (100 %) of 4.4. MS (ESI) 585.4 (MH+).
Example 9
Figure imgf000043_0001
(2E)-3-(5-{4-[(4'-chloro-4-methoxy-l,l'-biphenyl-2-yl)methoxy]phenyl}-l- cyclohexyl-lH-pyrazoI-3-yl)-2-methyl-2-propenoic acid (4.5). Ethyl ester 4.4 (55 mg, 0.094 mmol) was stirred with IN LiOH (1 ml) in 4 ml of MeOH/THF (1:3) at rt for 24 h, concentrated, and partitioned between Et2O and H2O. The organic phase was extracted with 0.1 N NaOH (2X), and the combined aqueous extracts were acidified with cone. HCl and extracted with EtOAc (3X). The combined organic extracts were washed with H2O, brine, and dried (Na2SO4). Concentration gave a crude product which was purified by semi-preparative HPLC (gradient elution: 70 to 100% CH3CN/H2O + 0.1% TFA) to give 4.5, 47 mg (90%). HRMS (ESI) calc'd for C33H34ClN2O4 577.2207, found 557.2218 (MH+); 1H NMR (300 MHz, CDC13) δ 7.83 (d, 7 = 1.1, IH), 7.39-7.25 (m, 7H), 7.19 (d, 7 = 2.5, IH), 7.00-6.95 (m, 3H), 6.47 (s, IH), 4.95 (s, 2H), 4.14-4.05 (m, IH), 3.88 (s, 3H), 2.24 (s, 3H), 2.10-1.98 (m, 2H), 1.94-1.87 (m, 4H), 1.27-1.22 (m, 4H). Example 10
Figure imgf000044_0001
Ethyl (2E)-3-(5-{4-[(t-butyl-2-bromo-5- phenylcarboxylate)methoxy]phenyl}-l-cyclohexyl-lH-pyrazol-3-yI)-2-methyI-2- propenoate (5.1). Phenol 4.3 (116 mg, 0.33 mmol) was dissolved in of DMF (3.2 ml), cesium carbonate (341 mg, 1.05mmol), and bromide (120mg, 0.34 mmol) were added, and the reaction was stirred for 18 h at rt under N2. Solvent was removed in vacuo, and the residue partitioned between ethyl acetate and water. The organic layer was washed with brine, dried (MgSO4), and concentrated. Purification by silica gel chromatography (stepwise gradient; 7% to 25% EtOAc / Hexanes) gave a colorless amorphous solid 124 mg (60%) of 5.1. MS (ESI) 625.3 (MH+). Example 11
Figure imgf000044_0002
Ethyl (2E)-3-(5-{4-[(4'-chloro-4-t-butoxycarbonyl-l,l'-biphenyl-2- yl)methoxy]phenyl}-l-cyclohexyl-lH-pyrazol-3-yl)-2-methyl-2-propenoate (5.2) . Bromide 5.1 (120mg, 0.19 mmol) and 4-chlorophenylboronic acid (33 mg, 0.21 mmol) were admixed in a 2 dram vial containing anhydrous THF (1.9 ml) and saturated NaHCO3 solution (1.1 ml) and the solution sparged with N2 prior to addition of tetrakis(triphenylphosphine) palladium 0 (24 mg, 0.02mmol). The reaction was heated to 80°C for 18 h, cooled, partitioned between EtOAc and water, and the organic layer washed with brine, and dried (MgSO4). Purificaion by silica gel chromatography (stepwise gradient: 5% to 25% EtOAc/Ηexanes) yielded 103 mg (82%) of 5.2. MS (ESI) 655.3 (MH+). Example 12
Figure imgf000045_0001
Ethyl (2E)-3-(5-{4-[(4'-Chloro-4-N-methylcarbamoyl-l,l,-biphenyl-2- yl)methoxy]phenyl}-l-cyclohexyl-lH-pyrazol-3-yl)-2-methyl-2-propenoate (5.3) . Ester 5.2 (103 mg, 0.157 mmol) was dissolved in dicholormethane (5 ml) and after trifluoroacetic acid (5 ml) was added under N2 the reaction was capped and stirred for 2 h. Volatiles were removed in vacuo (co-evaporate with toluene) and the residue was placed on high vacuum 1 h to remove trace moisture [MS (ESI) 599.2 (MH+)], and the dried was dissolved in DMF (2.5 ml). Diisopropylethylamine (0.186 ml), O- benzotriazol-l-yl-N,N,N',N'-tetramethyluronium tetrafluoroborate (TBTU) (85 mg, 0.26mmol) were added and the solution stirred for 10 min. An aliquot (0.675 ml, 0.043 mmol) of the solution was partitioned into 2 dram vial and N-methylamine (0.3 mmol, 7eq, gas) amine added. The reaction was stirred under N2 for 16 h, solvent removed in vacuo and residue purified by chromatography (ISCO Optix 10 system equipped with 4.2g silica gel redi-sep cartridges) to give 17.4 mg (66%) of 5.3. MS (ESI) 626.2 (MH+). Example 13
Figure imgf000046_0001
(2E)-3-(5-{4-[(4'-Chloro-4-N-methylcarbamoyl-l,l'-biphenyl-2- yl)methoxy]phenyl}-l-cyclohexyl-lH-pyrazoI-3-yl)-2-methyl-2-propenoic acid
(5.4). Ester 5.3 (12.1 mg, 0.02 mmol) was dissolved in THF (0.25 ml), IN sodium hydroxide (0.04 ml, 0.04 mmol) added, and the reaction mixture heated at 60°C for 5.5 h. The solvent was removed in vacuo and the residue partitioned between EtOAc and IN HCl. The organic layer was washed with brine and dried (MgSO4), and concentrated to give 11 mg (95%) of 5.4. HRMS (ESI) calc'd for C34H35ClN3O4 584.2316, found 584.2303 (MH+); IH NMR (300 MHz, CDC13) δ 8.05 (d, 7 = 2.5, IH), 7.80 (dd, 7 = 3.0, 9.1, IH), 7.78 (s, IH), 7.42-7.28 (m, 7H), 6.95 (d, 7 = 9.0, 2H), 6.43 (s, IH), 4.97 (s, 2H), 3.05 (d, 7 = 6.0, 3H), 2.25 (s, 3H), 2.10-1.65 (m, 6H), 1.29- 1.21 (m, 5H).
Example 14
Figure imgf000046_0002
Methyl 3-{5-[4-(benzyloxy)phenyl]-l-cyclohexyl-lH-pyrazol-3- yl}propanoate (6.1). To a solution of methyl ester 2.3 (52 mg, 0.125 mmol) in benzene (5 ml), was added 10 mg of 10% Pd-C. The atmosphere was evacuated and flushed with H2 gas (3X), and the solution stirred for 1.5 h. The mixture was filtered and concentrated to give 52 mg (100%) of 6.1. MS (ESI) 419.4 (M + H+). Example 15
Figure imgf000047_0001
3-{5-[4-(benzyIoxy)phenyl]-l-cycIohexyl-lH-pyrazol-3-yl}propanoic acid
(6.2). Methyl ester 6.1 (53 mg) was stirred with IN LiOH (2 ml) in 5 ml of MeOH/THF (4:1) over 18 h, concentrated, and partitioned between Et2O and H2O. The organic phase was extracted with 0.1 N NaOH (2X), and the combined aqueous extracts were acidified with cone. HCl and extracted with EtOAc (3X). The organic extracts were washed with H2O, brine, and dried (Na2SO4). The residue after concentration was triturated with hexanes/ Et2O (10:1) to afford acid 6.2, 39 mg (100%). HRMS (ESI) calc'd for C25H29N2O3 405.2100, found 405.2198 (MH+); MS (ESI); Η NMR (300 MHz, CDC13) δ 7.48-7.34 (m, 5H), 7.27 (d, 7 = 7.0, 2H), 7.06 (d, 7 = 8.7, 2H), 6.01 (s, IH), 5.13 (s, 2H), 4.06-3.98 (m, IH), 3.02-2.97 (m, 2H), 2.85-2.81 (m, 2H), 1.91-1.83 (m, 6H), 1.26 (br s, 4H).
Intermediate 6.3
Figure imgf000047_0002
Methyl 3-[l-cyclohexyl-5-(4-hydroxyphenyl)-lH-pyrazol-3-yl]propanoate
(6.3). To a solution of methyl ester 6.1 (71.5 mg, 0.172 mmol) in MeOH (4 ml) was added 20 mg 10% Pd-C prior to atmosphere evacuation and flushed with H (3X). The reaction mixture was stirred under H2 for 2 h, filtered, and concentrated to give 54 mg (96%) of 6.3. MS (ESI) 329.3 (MH+), 327.3 (MH"). Example 16
Figure imgf000048_0001
Methyl 3-(5-{4-[(4'-chloro-4-methoxy-l,l,-biphenyl-2- yl)methoxy]phenyl}-l-cyclohexyl-lH-pyrazol-3-yl)propanoate (6.4). A mixture 6.3 (39 mg, 0.119 mmol), 4'-chloro-4-methoxy-l,l'-biphenyl-2-yl)methyl bromide (44.4 mg, 0.143 mmol), and powdered K2CO3 (32.9 mg, 0.238 mmol) in CH3CN (2 ml) was heated at reflux for 1.5 h. The reaction mixture was filtered, poured into EtOAc, washed with water, brine, and dried (Na2SO4). Conentration purification by SiO2 chromatography (stepwise gradient: 20 to 25% EtOAc/hexanes) to afforded 6.4, 66 mg (100%). MS (ESI) 559.5 (MH+).
Example 17
Figure imgf000048_0002
3-(5-{4-[(4'-chloro-4-methoxy-l,l'-biphenyl-2-yl)methoxy]phenyl}-l- cyclohexyl-lH-pyrazol-3-yl)propanoic acid (6.5). Methyl ester 6.4 (50 mg, 0.092) was stirred with IN LiOH (2 ml) in 3 ml of MeOH/THF (2: 1) at rt for 15 h, concentrated, and partitioned between Et2O and H2O. The organic phase was extracted with 0.1 N NaOH (2X), and the combined aqueous extracts were acidified with cone. HCl and extracted with EtOAc (3X). The combined organic extracts were washed with H2O, brine, dried (Na2SO4). The residue from concentration was purified by SiO chromatography (stepwise gradient: 50 to 60 to 70%
EtOAc/ exanes) to yield 6.5, 31.6 mg (65%). HRMS (ESI) calc'd for C32H34ClN2O4 545.2129, found 545.2204 (MH+); 1H NMR (300 MHz, CDC13) δ 7.38-7.17 (m, 8H), 6.99-6.93 (m, 3H), 6.00 (2, IH), 4.94 (s, 2H), 4.05-3.97 (m, IH), 3.88 (s, 3H), 3.01- 2.97 (m, 2H), 2.85-2.80 (m, 2H), 1.90-1.83 (m, 6H), 1.25 (br s, 4H).
Intermediate 7.1
Figure imgf000049_0001
5-[4-(Benzyloxy)phenyI]-l-cyclohexyl-3-(2,2-dibromovinyl)-lH-pyrazole
(7.1). To a solution of CBr4 (916 mg, 2.76 mmol) in 7 ml of CH2C12 at 0°C, was added PPh3 (1.45 g, 5.52 mmol), and the mixture was stirred at 0°C for 10 min before addition of aldehyde 2.2 (500 mg, 1.38 mmol as a solution in 2 ml of CH2C12). The mixture was stirred at 0°C for 30 min, diluted with hexanes, filtered through a 1" pad of SiO2 eluting with 10% EtOAc/hexanes. The filtrate was concentrated and the resultant residue purified by SiO2 chromatography (5% EtOAc/hexanes) to afford 7.1, 648 mg (91%). MS (ESI) 515.1 (MH+).
Intermediate 7.2
Figure imgf000049_0002
5-[4-(benzyloxy)phenyl]-l-cyclohexyI-3-ethynyl-lH-pyrazole (7.2). n- Butyllithium (1.6 M BuLi in hexanes, 1.65 ml, 2.64 mmol) was added dropwise to a solution of 7.1 (620 mg, 1.20 mmol) in THF (6 ml) at -50°. The mixture was stirred at -50°C for 30 min, quenched with H2O, diluted with EtOAc, washed with IN HCl, H2O, brine, and dried (Na2SO4). The crude product was purified by SiO2 chromatography to afford 261 mg (61%) of 7.2. MS (ESI) 357.3 (MH+). Example 18
Figure imgf000050_0001
Ethyl 3-{5-[4-(benzyloxy)phenyl]-l-cyclohexyl-lH-pyrazol-3-yl}-2- propynoate (7.3). To a solution 7.2 (181 mg, 0.508 mmol) in 4 ml of THF at -78°C was added BuLi (1.6 M in hexanes, 349 μL, 0.559 mmol), and the solution was stirred at -78°C for 15 min, followed by additon of ethyl chloroformate (73 μL, 0.762 mmol). The mixture was allowed to warm to 0°C over 30 min, quenched with H2O, diluted with EtOAc, washed with H2O, brine, and dried (Na2SO4). The mixture was combined with another 0.141 mmol batch for purification by SiO2 chromatography (stepwise gradient: 5 to 7.5 to 10 to 12.5% EtOAc/hexanes) to give 7.3, 214 mg (77%). MS (ESI) 429.4 (MH+). Example 19
Figure imgf000050_0002
3-{5-[4-(benzyloxy)phenyl]-l-cyclohexyl-lH-pyrazol-3-yl}-2-propynoic acid (7.4). A mixture of ester 7.3 (68 mg, 0.157 mmol) and IN LiOΗ (2 ml) in 6 ml MeOΗ/TΗF (1:2) was stirred at rt for 24 h, concentrated, and partitioned between Et2O and Η2O. The organic phase was extracted with 0.1 N NaOH (2X), and the combined aqueous extracts were acidified with cone. HCl, and extracted with EtOAc (3X). The combined organic extracts were washed with H2O, brine, and dried (Na2SO4) to afford 64 mg (100%) of acid 7.4. HRMS (ESI) calc'd for C25H25N2O3 401.1865, found 401.1855 (M + H+); 1H NMR (300 MHz, CDC13) δ 7.48-7.37 (m, 5H), 7.26 (d, 7 = 8.8, 2H), 7.08 (d, 7 = 8.8, 2H), 6.51 (s, IH), 5.13 (s, 2H), 4.15-4.06 (m, IH), 2.05-1.97 (m, 2H), 1.90-1.87 (m, 4H), 1.28-1.23 (m, 4H). Intermediate 7.5
Figure imgf000051_0001
Ethyl 3-[l-cyclohexyl-5-(4-hydroxyphenyl)-lH-pyrazol-3-yl]-2- propynoate (7.5). A IM solution of BC13 in CH2C12 (1 ml, 1.00 mmol) was added to a solution of intermediate 7.3 (86 mg, 0.207 mmol) in 2 ml of CH2C12 at -50°C, and after being stirred at -50°C for 15 min, the solution was quenched with MeOH, diluted with EtOAc, washed with H2O, sat. NaHCO3, brine, and dried (Na2SO4). Filtration through a 1" pad of SiO2 and concentration provided 69 mg (100%) of 7.5. MS (ESI) 339.3 (MH+), 337.3 (MK).
Example 20
Figure imgf000051_0002
Ethyl 3-(5-{4-[(4'-chloro-4-methoxy-l,l'-biphenyl-2-yl)methoxy]phenyl}- l-cyclohexyl-lH-pyrazol-3-yl)-2-propynoate (7.6). A mixture of 7.5 (40.9 mg, 0.120 mmol), 4'-chloro-4-methoxy-l,l'-biphenyl-2-yl)methyl bromide (44.8 mg, 0.144 mmol), and powdered K2CO3 (33.2 mg, 0.240 mmol) in 2 ml of CΗ3CN was heated reflux for 2 h, poured into EtOAc, washed with water, brine, and dried (Na SO4). The product was purified by SiO2 chromatography (stepwise gradient: 5 to 7.5 to 10 to 12.5% EtOAc/hexanes) to afford 67 mg (98 %) of 7.6. MS (ESI) 569.3 (MH+). Example 21
Figure imgf000052_0001
3-(5-{4-[(4'-chloro-4-methoxy-l,l'-biphenyl-2-yl)methoxy]phenyl}-l- cyclohexyl-lH-pyrazol-3-yl)-2-propynoic acid (7.7). Ester 7.6 (55 mg, 0.097 mmol) was stirred with IN LiOH (1 ml) in 4 ml of MeOH/THF (1:3) at rt for 24 h, concentrated, and partitioned between Et O and H2O. The organic phase was extracted with 0.1 N NaOH (2X), and the combined aqueous extracts were acidified with cone. HCl, and extracted with EtOAc (3X). The combined organic extracts were washed with H O, brine, and dried (Na2SO ). The crude mixture was purified by semi-preparative HPLC (gradient elution: 70 to 100% CH3CN/H2O + 0.1% TFA) to give 7.7, 52 mg (100%). HRMS (ESI) calc'd for C32H30C1N2O4 514.1894, found 541.1913 (MH+); 1H NMR (300 MHz, CDC13) δ 7.39-7.17 (m, 8H), 7.00-6.94 (m, 3H), 6.51 (s, IH), 4.95 (s, 2H), 4.14-4.05 (m, IH), 3.88 (s, 3H), 2.08-1.96 (m, 2H), 1.90-1.86 (m, 4H), 1.26 (br s, 4H).
Example 22
Figure imgf000052_0002
Ethyl 5-[4-(benzyloxy)phenyl]-4-bromo-l-cyclohexyl-lH-pyrazole-3- carboxylate (8.1). Bromine (82 μL, 1.61 mmol) was added to pyrazole 1.3 (500 mg, 1.24 mmol) in AcOΗ (5 ml) at 0°C and the reaction mixture was stirred at rt for 17 h. Upon concentration, the crude product was purified by SiO2 chromatography
(stepwise gradient: 20 to 25% EtOAc/ hexanes) to afford 368 mg (62%) of bromide 8.1. MS (ESI) 483.4 (MΗ+). Intermediate 8.2
Figure imgf000053_0001
{5-[4-(benzyloxy)phenyl]-4-bromo-l-cyclohexyl-lH-pyrazol-3- yl}methanol (8.2). Alcohol 8.2 was prepared from 8.1 according to the procedure described above for the preparation of alcohol 2.1. The LAH reduction 8.2 in 310 mg (98%). MS (ESI) 441.4 (MH+). Intermediate 8.3
Figure imgf000053_0002
5-[4-(benzyloxy)phenyl]-4-bromo-l-cyclohexyl-lH-pyrazole-3- carbaldehyde (8.3). Aldehyde 8.3 was prepared from 8.2 according to the procedure described above for the preparation aldehyde 2.2. In this example, 8.2 (295 mg, 0.668 mmol) was oxidized to yield 242 mg (82%) of 8.3. MS (ESI) 439.3 (MH+).
Example 23
Figure imgf000053_0003
Methyl (2E)-3-{5-[4-(benzyloxy)phenyl]-4-bromo-l-cyclohexyl-lH- pyrazol-3-yl}-2-propenoate (8.4). Methyl ester 8.4 was prepared from 8.3 according to the procedure described above for the preparation ester 2.3. Thus, 8.3 (227 mg, 0.517 mmol) yielded 244 mg (95%) of ester 8.4. MS (ESI) 495.4 (MH+). Example 24
Figure imgf000054_0001
(2E)-3-{5-[4-(benzyloxy)phenyl]-4-bromo-l-cycIohexyl-lH-pyrazol-3-yl}-
2-propenoic acid (8.5). Acid 8.5 was prepared from 8.4 according to the procedure described above for the preparation acid 2.4. Thus, 8.4 (47 mg, 0.095 mmol) was saponified to yield 45 mg (99%) of 8.5. HRMS (ESI) calc'd for C25H26BrN2O3 481.1127, found 481.1105 (MH+); 1H NMR (300 MHz, CDC13) δ 7.78 (d, 7 = 16.1.1H), 7.49-7.34 (m, 5H), 7.27 (d, 7 = 8.8, 2H), 7.12 (d, 7 = 8.8, 2H), 6.88 (d, 7 = 16.1, IH), 5.14 (s, 2H), 4.04-3.96 (m, IH), 2.00-1.83 (m, 6H), 1.26-1.24 (m, 4H).
Example 25
Figure imgf000054_0002
Methyl (2E)-3-{5-[4-(benzyloxy)phenyl]-l-cyclohexyl-4-phenyl-lH- pyrazol-3-yl}-2-propenate (8.6). To a degassed mixture of bromide 8.4 (150 mg, 0.303 mmol), phenyl boronic (44 mg, 0.36 mmol), and tri-o-tolylphosphine (9.3 mg, 0.03 mmol), and NaHCO3 (105 mg, 1.25 mmol) in DME/H2O (3:1) was added
Pd(OAc)2 (cat.). The mixture was stirred at 90°C for 45 min, diluted with EtOAc, washed with water, brine, and dried (Na SO4). Purification by silica gel chromatography (stepwise gradient: 10 to 15% EtO Ac/hex) gave 115 mg (77%) of 8.6. MS (ESI) 493.5 (MH+). Example 26
Figure imgf000055_0001
(2E)-3-{5-[4-(benzyloxy)phenyl]-l-cyclohexyl-4-phenyl-lH-pyrazol-3-yl}-
2-propenoic acid (8.7). Acid 8.7 was prepared from ester 8.6 according to the procedure described above for the preparation acid 2.4. Thus, 8.6 (50 mg, 0.11 mmol) was saponified to yield 46 mg (95%) of 8.7. HRMS (ESI) calc'd for C31H31N2O3, 479.2335, found 479.2343 (MH+); 1H NMR (300 MHz, CDC13) δ 7.72 (d, 7 = 15.7, IH), 7.46-7.21 (m, 8H), 7.12-7.08 (m, 4H), 6.97 (d, 7 = 8.7, 2H), 6.60 (d, 7= 15.7, IH), 5.06 (s, 2H), 4.04-3.97 (m, IH), 2.10-1.85 (m, 5H), 1.65 (br s, IH), 1.33-1.22 (m, 4H).
Intermediate 8.8
Figure imgf000055_0002
Methyl (2E)-3-[l-cyclohexyl-4-phenyl-5-(4-hydroxyphenyl)-lH-pyrazol- 3-yl]-2-propenoate (8.8). Phenol 8.8 was prepared from 8.6 according to the procedure described above for the preparation phenol 3.1. Thus, 8.6 (60 mg, 0.12 mmol) was debenzylated to yield 49 mg (100%) of 8.8. MS (ESI) 403.3 (MH+), 401.3 (MIT). Example 27
Figure imgf000056_0001
Methyl (2E)-3-(5-{4-[4'-chloro-4-methoxy-l,l-biphenyl)methoxy]phenyl}- l-cyclohexyl-4-phenyl-5-lH-pyrazol-3-yl]-2-propenoate (8.9). Ester 8.9 was prepared from 8.8 according to the procedure described above for the preparation 3.2. Thus, 8.8 (49 mg, 0.12 mmol) was alkylated with (4'-chloro-4-methoxy-l,l'- biphenyl-2-yl)methyl bromide to afford 69.5 mg (90%) of 8.9. MS (ESI) 633.4 (MΗ+).
Example 28
Figure imgf000056_0002
(2E)-3-(5-{4-[4'-chloro-4-methoxy-l,l-biphenyl)methoxy]phenyl}-l- cyclohexyl-4-phenyl-5-lH-pyrazol-3-yl]-2-propenoic acid (8.10). Ester 8.9 was saponified to 8.10 according to the procedure described above for the preparation acid 3.3. Thus, 8.9 (64 mg, 0.10 mmol) was hydrolyzed to afford 53 mg (85%) of 8.10. HRMS (ESI) calc'd for C38H36C1N204, 619.2364, found 619.2390 (MH+); Η NMR (300 MHz, CDC13) δ 7.72 (d, 7 = 15.7, IH), 7.37-7.22 (m, 8H), 7.16 (d, 7 = 2.9, IH), 7.07 (d, 7 = 8.4, IH), 6.96 (dd, 7 = 8.5, 2.5, IH), 6.85 (d, 7 = 8.8, 2H), 6.60 (d, 7 = 15.7, IH), 4.88 (s, 2H), 4.03-3.94 (m, IH), 3.87 (s, 3H), 2.09-1.85 (m, 5H), 1.66 (br s, IH), 1.29-1.20 (m, 4H). Intermediate 9.1
Figure imgf000057_0001
Ethyl (2Z)-4-cyclohexyl-2-hydroxy-4-oxo-2-butenoate (9.1). To a solution of cyclohexyl methyl ketone (10.4 g, 82.4 mmol) in DMF (90 ml) at rt, was added 60% NaH (3.96 g, 98.9 mmol). The mixture was stirred 45 min, cooled to 0°C, diethyl oxalate (13.4 ml, 98.9 mmol) was added and the temperature allowed to warm and stay at rt for 3 h. The reaction mixture was poured into Et O, extracted with H2O (3X), and the aqueous phase was acidified with cone. HCl and extracted with EtOAc (3X). The organic extracts were washed with brine, dried (Na2SO ), and concentrated to afford 17.45 g (94%) of 9.1. MS (ESI) 225.2 (MH+).
Example 29
Figure imgf000057_0002
Ethyl 1 - [4- (benzy loxy )pheny 1] -5-cyclohexyl- lH-py razole-3-carboxy late
(9.2). According to the procedure for preparation of 1.3, butenoate 9.1 (1 g, 4.42 mmol) and 4-benzyloxyhydrazine hydrochloride (1.33 g, 5.30 mmol) were combined and heated at reflux in EtOH (13 ml) for lh. The mixture was filtered, concentrated, and the resultant residue purified by SiO2 chromatography (stepwise gradient, 10 to 35% EtOAc/hexanes) to afford 845 mg (47%) of 9.2. MS (ESI) 405.4 (MH+). Intermediate 9.3
Figure imgf000058_0001
(2E)-3-{l-[4-(benzyloxy)phenyl]-5-cyclohexyl-lH-pyrazol-3-yl}-2- propenoic acid (9.3). Reduction of 9.2 (1 g, 2.47 mmol) was performed according to preparation of 2.1. Thus, 785 mg (83%) of 9.3 was obtained. MS (ESI) 363.4 (MH+).
Intermediate 9.4
Figure imgf000058_0002
l-[4-(benzyloxy)phenyl]-5-cyclohexyl-lH-pyrazole-3-carbaldehyde (9.4).
According to the procedure for preparation of 2.2, alcohol 9.3 (717 mg, 1.98 mmol) was oxidized to yield 675 mg (95%) of 9.4. HRMS (ESI) calc'd for C23H25N2O2 361.1916, found 361.1914 (MH+).
Example 30
Figure imgf000058_0003
Methyl (2E)-3-{l-[4-(benzyloxy)phenyl]-5-cycIohexyl-lH-pyrazol-3-yl}-2- propenoate (9.5). According to the procedure for preparation of 2.3, aldehyde 9.4 (623 mg, 1.73 mmol) was reacted with methyl (triphenylphosphoranylidene)acetate (867 mg, 2.59 mmol) to yield 536 mg (74%) of 9.5. MS (ESI) 855.8 (2M + Na+).
Example 31
Figure imgf000059_0001
According to the procedure for preparation of 2.4, ester 9.5 (50 mg, 0.120 mmol) was saponified to yield 42 mg (88%) of 9.6. HRMS (ESI) calc'd for C25H27N2O3 403.2022, found 403.2026 (MH+); 1H NMR (300 MHz, CDC13) δ 7.77 (d, 7 = 16.1, IH), 7.47-7.31 (m, 7H), 7.07 (d, 7 = 8.8, 2H), 6.45 (s, IH), 6.43 (d, 7 = 16.1, IH), 5.13 (s, 2H), 2.61-2.53 (m, IH), 1.86-1.68 (m, 5H), 1.39-1.19 (m, 5H).
Intermediate 9.7
Figure imgf000059_0002
Methyl (2E)-3-[5-cyclohexyl-l-(4-hydroxyphenyl)-lH-pyrazol-3-yl]-2- propenoate (9.7). According to the procedure for preparation of 3.1, debenzylation of 9.5 (100 mg, 0.240 mmol) with BC13 (1.20 mmol) to yielded 70 mg (90%) of 9.7. MS (ESI) 653.7 (2MH+); (2M+Na+). Example 32
Figure imgf000060_0001
Methyl (2E)-3-(l-{4-[(4'-chloro-4-methoxy-l,l,-biphenyl-2- yl)methoxy]phenyl}-5-cyclohexyl-lH-pyrazol-3-yl)-2-propenoate (9.8).
According to the procedure for preparation of 3.2, phenol 9.7 (55 mg, 0.169 mmol) was alkylated with 4'-chloro-4-methoxy-l,l'-biphenyl-2-yl)methyl bromide (63.0 mg, 0.202 mmol) to yield 87 mg (93%) of 9.8. MS (ESI) 557.5 (MH+).
Example 33
Figure imgf000060_0002
(2E)-3-(l-{4-[(4'-chloro-4-methoxy-l,l'-biphenyl-2-yl)methoxy]phenyl}-5- cyclohexyl-lH-pyrazol-3-yl)-2-propenoic acid (9.9). According to the procedure for the preparation of 3.3, ester 9.8 (72 mg, 0.129 mmol) was saponified to yield 50 mg (71%) of 9.9. HRMS (ESI) calc'd for C32H32ClN2O4 543.2051, found 543.2021 (MH+); 1H NMR (300 MHz, CDC13) δ 7.76 (d, 7 = 16.1, IH), 7.38-7.24 (m, 7H), 7.17 (d, 7 = 2.5, IH), 6.99-6.93 (m, 3H), 6.44 (s, IH), 6.43 (d, 7 = 15.7, IH), 4.95 (s, 2H), 3.87 (s, 3H), 2.55-2.51 (m, IH), 1.81-1.68 (m, 5H), 1.38-1.21 (m, 5H). Example 34
Figure imgf000061_0001
Ethyl (2E)-3-{l-[4-(benzyloxy)phenyl]-5-cyclohexyl-lH-pyrazol-3-yl}-2- methyl-2-propenoate (10.1). According to the procedure for preparation of 4.1, aldehyde 9.4 (500 mg, 1.39 mmol) was treated with (1- ethyoxycorbonylethyl)triphenylphosphonium bromide (739.6 mg, 1.67 mmol) to give 537 mg (87%) of 10.1. MS (ESI) 445.2 (MH+).
Example 35
Figure imgf000061_0002
(2E) -3-{ 1 - [4- (benzyloxy )pheny 1] -5-cyclohexyl- lH-py razol-3-yl }-2-methyl-
2-propenoic acid (10.2). Ethyl ester 10.1 was subject to saponification according to the procedure for preparation of 4.2 to give 41.9 mg (90%) of 10.2. HRMS (ESI) calc'd for C26H29N2O3 417.2178, found 417.2167 (MH+); Η NMR (300 MHz, CDC13) δ 7.70 (s, IH), 7.48-7.31 (m, 7H), 7.07 (d, 7 = 29.1, 2H), 6.42 (s, IH), 5.13 (s, 2H), 2.65-2.57 (m, IH), 2.27 (s, 3H), 1.88-1.67 (m, 5H), 1.41-1.22 (m, 5H). Intermediate 10.3
Figure imgf000062_0001
Ethyl (2E)-3-[5-cyclohexyl-l-(4-hydroxyphenyl)-lH-pyrazol-3-yl]-2- methyl-2-propenoate (10.3). According to the procedure for preparation of 4.3, debenzylation of 10.1 (440 mg, 0.99 mmol) with IM BCl3 in dichloromethane (4.95 ml, 4.95 mmol) yielded 353 mg (100%) of 10.3. MS (ESI) 355.4 (MH+).
Example 36
Figure imgf000062_0002
Ethyl (2E)-3-(l-{4-[(4,-chloro-4-methoxy-l,l,-biphenyl-2- yl)methoxy]phenyl}-5-cycIohexyl-lH-pyrazol-3-yl)-2-methyl-2-propenoate
(10.4). According to the procedure for preparation of 4.4, phenol 10.3 (50 mg, 0.14 mmol) was alkylated with 4'-chloro-4-methoxy-l,l'-biphenyl-2-yl)methyl bromide (51 mg, 0.17 mmol) to yield 78.8 mg (96%) of 10.4. MS (ESI) 585.4 (MΗ+). Example 37
Figure imgf000063_0001
(2E)-3-(l-{4-[(4,-chloro-4-methoxy-l,l'-biphenyl-2-yl)methoxy]phenyl}-5- cyclohexyl-lH-pyrazol-3-yl)-2-methyl-2-propenoic acid (10.5). According to the procedure for the preparation of 4.5, ester 10.4 (53 mg, 0.091 mmol) was saponified to yield 20 mg (39%) of 10.5. MS (ESI) 557.4 (MH+); 1H NMR (300 MHz, CDC13) δ 7.76 (s, IH), 7.39-7.24 (m, 6H), 7.18 (d, 7 = 2.5, IH), 7.00-6.96 (m, 4H), 6.51 (s, IH), 4.95 (s, 2H), 3.88 (s, 3H), 2.61-2.49 (m, IH), 2.23 (s, 3H), 1.87-1.71 (m, 5H), 1.43-1.22 (m, 5H).
Example 38
Figure imgf000063_0002
Ethyl (2E)-l-[4-(4'-Chloro-4-methylcarbamoyl-biphenyl-2-ylmethoxy)- phenyl]-5-cyclohexyl-lH-pyrazol-3-yl]-2-methyl-2-propenoate (10.6). Phenol 10.3 (44.3 mg, 0.125 mmol) was alkylated with 2-(bromomethyl)-4'chloro-N- methyl-l,l'-biphenyl-4-carboxamide (50 mg, 0.15 mmol; see reagent preparation below) according the preparation of 4.4 to provide 51 mg (59%) of amide 10.6. MS (ESI) 612.4 (MH+).
Example 39
Figure imgf000064_0001
(2E)-l-[4-(4'-Chloro-4-methylcarbamoyl-biphenyl-2-ylmethoxy)-phenyl]- 5-cyclohexyl-lH-pyrazol-3-yl]-2-methyI-2-propenoic acid (10.7). According to the procedure for preparation of 4.5, ester 10.6 (40 mg, 0.065 mmol) was saponified to give 30.5 mg (80%) of 10.7. HRMS (ESI) calc'd for C34H35ClN3O4 584.2316, found 584.2324 (MH+);1H NMR (300 MHz, CDC13) δ 8.02 (d, 7 = 2.5, IH), 7.82 (dd, 7 = 2.0, 7.9, IH), 7.76 (d, 7 = 1.1, IH), 7.43-7.28 (m, 7H), 6.95 (d, 7 = 8.8, 2H), 6.42 (s, IH), 4.99 (s, 2H), 3.07 (d, 7 = 3.7, 3H), 2.62-2.50 (m, IH), 2.24 (d. 7 = 1.1, 3H), 1.87-1.69 (m, 5H), 1.41-1.22 (m, 5H).
Preparation of 2-(bromomethyl)-4'chloro-N-methyl- 1,1' -biphenyl-4-carboxamide
Figure imgf000065_0001
Figure imgf000065_0002
2-(bromomethyl)-4'chloro-N-methyl-l,l'-biphenyl-4-carboxanιide. A suspension of 4-bromo-3-methylbenzoic acid (10 g, 46.5 mmol) was heated to 80°C and N,N-dimethylformamide di-tert-butyl acetal (44.6 ml, 186 mmol) was added dropwise over 30 min under N2 atmosphere. After being stirred 1.5 h, the reaction mixture was allowed to cool, washed with water, saturated NaHCO3, brine, and dried (Na2SO4). Concentration afforded an off-white oil 8.8 g (70%) of tert-butyl 4- bromo-3-methylbenzoate. To a solution of this material (3.3 g, 12.2 mmol) in 3:1 dimethoxyethane/water (55 ml) was added 4-chlorophenyl boronic acid (2.3 g, 14.6 mmol), tri-o-tolylphosphine (371 mg, 1.22 mmol), NaHCO3 (4 g, 48.8 mmol). After being degassed for 20 min by a bubbling stream of N , palladium acetate (137 mg, 0.6 mmol) was added, and the reaction heated at 90°C for 2.5 h under N2. The reaction mixture was cooled, and the organic layer washed with water, brine, and dried MgSO4. Concentration gave a residue which was suspended in hexane and filtered to remove a precipitate which was washed well with 9:1 hexane/ethyl acetate mixture, and the combined filtrates concentrated. The residue from filtrate concentrataion was subject to Silica gel chromatography (stepwise gradient 0 - 10% EtOAc/hexane) to afford tert-butyl-4'-chloro-2-methyl- 1,1 '-biphenyl -4-carboxylate as a white solid 3.7 g (99%). MS (ESI) 303.1 (MH+). To a solution of this material (3.7 g, 12.2 mmol) in dichloromethane (92 ml) at 0°C was added trifluoroacetate (92 ml) dropwise over 10 min. The solution was warmed to rt and stirred lh prior to concentration (co-evaporation with toluene, 2x50 ml) to give 4'-chloro-2-methyl- l,l'-biphenyl-4-carboxylate as a white solid 3 g (100%). MS (ESI) 247.1 (MH+). To a solution of this material (1.5 g, 6.0 mmol), 2M methyl amine in tetrahydrofuran (6 ml, 12. Mmol), and diisopropylamine (3.2 ml, 18 mmol) in dimethylformamide (30 ml) was added benzotriazol-l-yloxyltris(dimethylamino)- phosphonium hexafluorophosphate (3.22 g, 7.3 mmol) under a N2 atmosphere. The reaction mixture was stirred at rt for 1 h, diluted with dichloromethane (100 ml), washed with water (2x30 ml), saturated NaHCO3 (2x25 ml), brine, and dried (Na2SO4). Concentration and silica gel chromatography (stepwise gradient 50 - 100%
CH2Cl2/hexane) to obtain 4'-chloro-N-methyl-l,l'-biphenyl-4-carboxamide. A sample of this material was dissolved in dichloromethane (30 ml) and N- bromosuccinamide (2.14 g, 12 mmol) was added and the reaction irradiated with ultraviolet light for 4 h under reflux and N2 atmosphere. Concentration and purification by reverse phase HPLC (C18 column; water/acetonitrile/0.05% TFA; gradient) afforded 2-(bromomethyl)-4'chloro-N-methyl- 1,1' -biphenyl-4- carboxamide.
Figure imgf000066_0001
Methyl 3-{l-[4-(benzyloxy)phenyl]-5-cyclohexyl-lH-pyrazol-3- yljpropanoate (11.1). According to the procedure for preparation 6.1, propenoate 9.5 (149 mg, 0.358 mmol) was hydrogentaed to yield 144 mg (96%) of 11.1. MS(ESI) 419.4 (MH+). Example 41
Figure imgf000067_0001
3-{l-[4-(benzyloxy)phenyl]-5-cyclohexyl-lH-pyrazol-3-yl}propenoic acid
(11.2). According to the procedure for preparation of 6.2, ester 11.1 (44 mg, 0.105 mmol) was saponified to yield 39 mg (92%) of 11.2. HRMS (ESI) calc'd for C25H29N2O3 405.2178, found 405.2192 (MH+); 1H NMR (300 MHz, CDC13) δ 7.48- 7.33 (m, 5H), 7.28 (d, 7 = 8.1, 2H), 7.04 (d, 7 = 8.8, 2H), 6.00 (s, IH), 5.12 (s, 2H), 3.01-2.96 (m, 2H), 2.82-2.78 (m, 2H), 2.59-2.51 (m, IH), 1.84-1.67 (m, 5H), 1.36- 1.20 (m, 5H).
Intermediate 11.3
Figure imgf000067_0002
Methyl 3-[5-cyclohexyl-l-(4-hydroxyphenyl)-lH-pyrazol-3-yl]propanoate (11.3). According to the procedure for the preparation of 6.3, benzyl ether 11.2 (124 mg, 0.296 mmol) was cleaved by catalytic hydrogenation to yield 95 mg (98%) of 11.3. MS (ESI) 657.7 (2MH+).
Figure imgf000068_0001
Methyl 3-(l-{4-[(4'-chloro-4-methoxy-l,l'-biphenyl-2- yl)methoxy]phenyl}-5-cycIohexyl-lH-pyrazol-3-yl)propanoate (11.4). According to the procedure for preparation of 6.4, phenol 11.3 (83 mg, 0.253 mmol) was alkylated with 4'-chloro-4-methoxy-l,l'-biphenyl-2-yl)methyl bromide (95 mg, 0.303 mmol) to yield 133 mg (94%) of 11.4. MS (ESI) 581.5 (M + Na+).
Example 43
Figure imgf000068_0002
3-(l-{4-[(4'-chloro-4-nιethoxy-l,l'-biphenyl-2-yl)methoxy]phenyl}-5- cyclohexyl-lH-pyrazol-3-yl)propanoic acid (11.5). According to the procedure for preparation of 6.5, ester 11.4 (108 mg, 0.193 mmol) was saponified to yield 96 mg (91%) of 11.5. HRMS (ESI) calc'd for C32H34ClN2O4545.2207, found 545.2226 (MH+); Η NMR (300 MHz, CDC13) δ 7.38-7.17 (m, 8H), 6.99-6.91 (m, 3H), 6.00 (s, IH), 4.94 (s, 2H), 3.88 (s, 3H), 3.01-2.96 (m, 2H), 2.82-2.78 (m, 2H), 2.60-2.50 (m, IH), 1.83-1.67 (m, 5H), 1.35-1.20 (m, 5H). Intermediate 12.1
Figure imgf000069_0001
1-Cyclohexyl-lH-imidazole (12.1). Compound 12.1 was prepared in 44% yield by the procedure of Gridnev, A. A.; Mihaltseva, M. I. Synthesis of 1- Alkylimidazoles. Syn. Comm.. 1994, 24, 1547-1555.
Intermediate 12.2
Figure imgf000069_0002
2,4,5-tribromo-l-cyclohexyl-lH-imidazole (12.2.). N-bromosuccinamide (1.78 g, 9.99 mmol) dissolved in CHC1 (4 ml) at 0°C was added to a 4 ml solution of 12.1 (500 mg, 3.33 mmol) in CHC13. The mixture was stirred at rt for 17 h, filtered, concentrated, and the crude residue stirred in ether for 30 min. Filtration and concentration gave a crude product which was purified by SiO2 chromatography (10% EtOAc/hexanes) to afford 267 mg (21%) 12.2. MS (ESI) 385.0 (MH+). Intermediate 12.3
Figure imgf000069_0003
2-[4-(benzyloxy)phenyl]-4,5-dibromo-l-cyclohexyl-lH-imidazole (12.3). To a solution of 12.2 (1.99 g, 5.14 mmol) in 5:1 benzene/MeOH (24 ml) was added Na2CO3 (2M in water, 5.14 ml, 10.3 mmol) and 4-benzyloxyphenylboronic acid (1.23 g, 5.40mmol). The mixture was degassed over 10 min with N2 and tetrakis(triphenyl-phosphine)palladium (416 mg, 0.360 mmol) added, and the reaction mixture stirred at 65°C for 12 h before being partitioned between EtOAc and H2O. The organic phase was washed with brine, dried (Na2SO4), concentrated, and the crude product purified by SiO2 chromatography (10% EtOAc/hexanes) to afford 1.96 g (78%) of 12.3. MS (ESI) 489.2 (MH+).
Intermediate 12.4
Figure imgf000070_0001
2-[4-(benzyloxy)phenyl]-4-bromo-l-cyclohexyl-lH-imidazoIe (12.4). To a solution of 12.3 (1 g, 2.04 mmol) in THF (11 ml) at -78°C was added 1.6 M BuLi in hexanes (1.59 ml, 2.55 mmol). The mixture was stirred at -78°C for 0.5 h, quenched with H O, warmed to rt, and partitioned between EtOAc and H2O. The organic phase was washed with brine, dried (Na SO4), concentrated, and the crude product purified by SiO2 chromatography (stepwise gradient: 10 to 15 to 25% EtOAc/hexanes) to afford 723 mg (86%) of 12.4. MS (ESI) 411.3 (MH+).
Example 44
Figure imgf000070_0002
tert-butyl (2E)-3-{2-[4-(benzyloxy)phenyl]-l-cyclohexyl-lH-imidazol-4- yl}-2-propenoate (12.5). To a solution of 12.4 (100 mg, 0.243 mmol) in DMF (1.5 ml ) was added tert-butyl acrylate (0.045 ml, 0.304 mmol) and tri-o-tolylphosphine (3.7 mg, 0.0122 mmol). After the 5 min degass with N2, triethylamine (0.068 ml, 0.486 mmol) and Pd(OAc)2 (2.7 mg, 0.0122 mmol) were added, and the reaction mixture stirred at 80°C for 17 h. Additional reagents (same molar equivalents except triethylamine (0.034 ml, 0.243 mmol) and the mixture was stirred at 100°C for an additional 3 h. Reagent addition was repeated and the mixture again stirred at 100°C for 17 h. The solution was partitioned between EtOAc and H2O and the organic phase washed with brine, dried (Na2SO ), concentrated, and the crude product purified by SiO2 chromatography (stepwise gradient: 30 to 50% Et O/ hexanes) to afford 67 mg (60%) of 12.5. MS (ESI) 459.4 (MH+).
Example 45
Figure imgf000071_0001
(2E)-3-{2-[4-(benzyloxy)phenyl]-l-cyclohexyl-lH-imidazoI-4-yl}-2- propenoic acid (12.6). To a solution of 12.5 (53 mg, 0.116 mmol) in CH2C1 (2 ml) was added trifluoroacetic acid (3 ml) and the mixture was stirred for 17 h and concentrated. The crude product was purified by semi-preparative HPLC (gradient elution: 50 to 100% CH3CN/H2O + 0.1 % TFA) to afford 43 mg (72%) of 12.6.
HRMS (ESI) calc'd for C25H27N2O3 403.2022, found 403.2026 (M + H+); 1H NMR (300 MHz, CDC13) δ 7.59-7.56 (m, 3H), 7.46-7.34 (m, 6H), 7.13 (d, 7 = 8.4, 2H), 6.74 (d, 7 = 15.7, IH), 5.12 (s, 2H), 4.20-4.11 (m, IH), 2.07-2.03 (m, 2H), 1.95-1.89 (m, 2H), 1.75-1.71 (m, 3H), 1.28-1.25 (m, 3H). Intermediate 12.7
Figure imgf000072_0001
2-[4-(benzyloxy)phenyl]-4-carboxaldehyde-l-cyclohexyl-lH-imidazole
(12.7). To a solution of 12.4 (683 mg, 1.66 mmol), in THF (7 ml) at -78°C, was added tert-BuLi (1.7 M in pentane, 2.25 ml, 3.82 mmol). The mixture was stirred at - 78°C for 0.5 h, quenched with DMF (0.64 ml, 8.30 mmol), and allowed to warm to rt. Saturated NH4C1 solution (1 ml) was added, and the resulting mixture partitioned between EtOAc and H2O. The organic phase was washed with brine, dried
(Na2SO4), concentrated, and the crude product purified by silica gel chromatography (35% EtOAc/hexanes) to afford 387 mg (65%) of 12.7. MS (ESI) 361.3 (MH+).
Example 46
Figure imgf000072_0002
Methyl (2E)-3-{2-[4-(benzyloxy)phenyI]-l-cyclohexyl-lH-imidazol-4-yl}- 2-propenoate (12.8). According to the procedure for preparation of 4.1, aldehyde 12.7 (150 mg, 0.416 mmol) was reacted with methyl
(triphenylphosphoranylidene)acetate (209 mg, 0.624 mmol) to yield 128 mg (74%) of 12.8. MS (ESI) 417.3 (MΗ+). Intermediate 12.9
Figure imgf000073_0001
Methyl (2E)-3-{2-[4-(hydroxy)phenyl]-l-cyclohexyl-lH-imidazol-4-yl}-2- methyl-2-propenoate (12.9). According to the procedure for preparation of 4.3, ester 12.8 (117 mg, 0.281 mmol) was reacted with BC13 (1.40 mmol) to yield 69 mg (75%) of 12.9. MS (ESI) 327.3 (MH+).
Example 47
Figure imgf000073_0002
Methyl (2E)-3-(2-{4-[(4'-chloro-4-methoxy-l,l'-biphenyl-2- yl)methoxy]phenyl}-l-cyclohexyl-lH-imidazol-4-yl)-2-propenoate (12.10).
According to the procedure for preparation of 4.4, phenol 12.9 (60 mg, 0.184 mmol) was alkylated with (4'-chloro-4-methoxy-l,l'-biphenyl-2-yl)methyl bromide (63 mg, 0.202 mmol), to yield 75 mg (73%) of 12.10. MS (ESI) 557.4 (MH+). Example 48
Figure imgf000074_0001
(2E)-3-(2-{4-[(4'-chloro-4-methoxy-l,l,-biphenyI-2-yl)methoxy]phenyl}-l- cyclohexyI-lH-imidazol-4-yl)-2-propenoic acid (12.11). According to the procedure for preparation of 4.5, ester 12.10 (60 mg, 0.108 mmol) was saponified to yield 56 mg (79%) of 12.11. HRMS (ESI) calc'd for C32H32ClN2O4 543.2051, found 543.2005 (M + H+); IH NMR (300 MHz, CDC13) δ 7.56-7.53 (m, 3H), 7.43-7.24 (m, 6H), 7.14 (d, J = 3.0, IH), 7.03-6.95 (m, 3H), 6.74 (d, J = 16.1, IH), 4.95 (s, 2H), 4.21-4.11 (m, IH), 3.86 (s, 3H), 2.07-2.03 (m, 2H), 1.89-1.86 (m, 2H), 1.80-1.65 (m, 3H), 1.29-1.23 (m, 3H).
Example 49
Figure imgf000074_0002
Ethyl (2E)-3-{2-[4-(benzyloxy)phenyl]-l-cyclohexyl-lH-imidazol-4-yl}-2- methyl-2-propenoate (13.1). According to the procedure for preparation of 4.1, aldehyde 12.7 (225 mg, 0.624 mmol) was reacted with (1- ethoxycarbonylethyl)triphenylphosponium bromide (346 mg, 0.780 mmol) to yield 234 mg (85%) of 13.1. MS (ESI) 445.5 (MH+). Example 50
Figure imgf000075_0001
(2E)-3-{2-[4-(benzyloxy)phenyl]-l-cyclohexyl-lH-imidazol-4-yl}-2- methyl-2-propenoic acid (13.2.). According to the procedure for preparation of 4.2, ester 13.1 (50 mg, 0.112 mmol) was saponified to yield 38 mg (64%) of 13.2. HRMS (ESI) calc'd for C26H29N2O3417.2178, found 417.2180 (MH+); IH NMR (300 MHz, CDC13) δ 7.60-7.57 (m, 3H), 7.46-7.35 (m, 6H), 7.14 (d, J = 8.4, 2H), 5.12 (s, 2H), 4.20-4.16 (m, IH), 2.10-2.05 (m, 5H), 1.91-1.74 (m, 5H), 1.31-1.29 (m, 3H).
Intermediate 13.3
Figure imgf000075_0002
Ethyl (2E)-3-{2-[4-(hydroxy)phenyl]-l-cyclohexyl-lH-imidazol-4-yl}-2- methyl-2-propenoate (13.3). According to the procedure for preparation of 4.3, ester 13.1 (167 mg, 0.376 mmol) was treated with BC13 (1.88 mmol) to yield 80 mg (60%) of 13.3. MS (ESI) 355.3 (MH+). Example 51
Figure imgf000076_0001
Ethyl (2E)-3-(2-{4-[(4'-chloro-4-methoxy-l,l*-biphenyl-2- yl)methoxy]phenyl}-l-cyclohexyl-lH-imidazol-4-yl)-2-methyl-2-propenoate
(13.4). According to the procedure for preparation of 4.4, phenol 13.3 (73 mg, 0.206 mmol) was alkylated with (4'-chloro-4-methoxy-l,l'-biphenyl-2-yl)methyl bromide (71 mg, 0.227 mmol), to yield 59 mg (49%) of 13.4. MS (ESI) 585.4 (MH+).
Example 52
Figure imgf000076_0002
(2E)-3-(2-{4-[(4'-chloro-4-methoxy-l,l,-biphenyl-2-yl)methoxy]phenyl}-l- cyclohexyl-lH-imidazol-4-yl)-2-methyl-2-propenoic acid (13.5). According to the procedure for preparation of 4.5, ester 13.4 (55 mg, 0.0940 mmol) was saponified to yield 42 mg (67%) of 13.5. HRMS (ESI) calc'd for C33H34ClN2O4557.2207, found 557.2216 (MH+); IH NMR (300 MHz, CDC13) δ 7.57-7.54 (m, 3H), 7.42-7.24 (m, 6H), 7.15 (d, J = 2.5, IH), 7.03 (d, J = 8.8, 2H), 6.97 (d,d J = 8.5, 2.6, IH), 4.95 (s, 2H), 4.23-4.11 (m, IH), 3.87 (s, 3H), 2.11-2.03 (m, 5H), 1.95-1.93 (m, 2H), 1.80- 1.76 (m, 3H), 1.40-1.21 (m, 3H).
Intermediate 13.6
Figure imgf000077_0001
t-Butyl (2E)-3-{2-[4-(hydroxy)phenyl]-l-cyclohexyl-lH-imidazol-4-yl}-2- methyl-2-propenoate (13.6). According to the procedure for preparation of 4.1, aldehyde 12.7 (300 mg, 0.83 mmol) was reacted with (1-t-butoxycarbonylethyl)- triphenylphosponium bromide (350 mg, 0.88 mmol), and the resulting product was dissolved in ethanol (2 ml) and 10% Pd/C (400 mg) added followed by addition of 1,4-cyclhexadiene (excess 0.8 g). The reaction mixture was stirred at 45°C forlO h, filtered, and concentrated to yield 190 mg (60%) of 13.6. MS (ESI) 383.27 (MH+).
Example 53
Figure imgf000077_0002
Butoxyt (2E)-3-(2-{4-[(4,-chIoro-4-N-methylcarbamoyl-l,l'-biphenyl-2- yl)methoxy]phenyl}-l-cyclohexyl-lH-imidazol-4-yl)-2-methyl-2-propenoate. Ester 13.6 (20 mg, 0.052 mmol), 2-(hydroxylmethyl)-4'chloro-N-methyl-l,l'- biphenyl-4-caroxamide (14.4 mg, 0.52 mmol), and triphenylphosphene (16.5 mg, 0.063 mmol) were taken up in THF (0.25 ml) and cooled to 0°C under N2 and diisopropylazo-dicarboxylate (12 mg, 0.06 mmol), dissolved in an equal volume of the same solvent, was added dropwise. The reaction was allowed to stand at rt for 18 h and an addition equivalent of each reagent was added to the reaction mixture. Water (3 drops) was added, concentrate to remove volatiles, and subject to reverse phase HPLC as above to yield 26 mg (66%) of 13.7. MS (ESI) 640.19 (MH+).
Example 54
(2E)-3-(2-{4-[(4,-chloro-4-N-methylcarbamoyl-l,l'-biphenyI-2- yl)methoxy]phenyl}-l-cyclohexyl-lH-imidazol-4-yl)-2-methyl-2-propenoic acid.
According to the procedure for preparation of 12.6, ester 13.7 (20 mg, 0.031 mmol) was cleaved to acid to give 22.5 mg (100%) of 13.8 as TFA salt. HRMS (ESI) calc'd for C34H34C1N3O4 584.2316, found 584.2319 (MH+); IH NMR (300 MHz, CD30D) δ 8.05-8.10 (m, 2H), 7.87-7.90 (m, IH), 7.60 (d, J = 8.8, 2H), 7.42-7.46 (m, 6H), 7.17 (d, J = 8.8, 2H), 5.12 (s, 2H), 4.24-4.28 (m, IH), 3.30 (s, 3H), 2.94 (s, 3H), 1.91-2.06 (m, 6H), 1.27-1.37 (m, 4H).

Claims

We claim:
A compound of Formula (I)
Figure imgf000079_0001
where:
Figure imgf000079_0002
A is CO2R5, CONSO2R5, SO2NCOR5,
Figure imgf000079_0003
Figure imgf000079_0004
Het is pyrazole, imidazole, oxazole, triazole, thiazole, pyrrole, furan, or thiophene;
X is O, S, NR5, or CH2;
R is hydrogen, halogen, Cj-όalkyl, trifluoromethyl, or phenyl;
R >2 i •s C3- cycloalkyl or Cs-12 bridged bicycloalkyl;
R is hydrogen, halogen, Cι-6alkyl, or Cι-6alkoxy;
Figure imgf000079_0005
R5 is hydrogen or Cι-6alkyl;
R6 is hydrogen, methyl, or OR5;
R7 is Cι.6alkoxy, cyano, trifluoromethyl, -CO2R5, -CONR9R10, SO2R5, or SO2NR9R10;
R8 is hydrogen, halogen, C1-6alkyl, C^alkoxy, cyano, trifluoromethyl, aceto, CO2R5, or CONR9R10;
R and R10 are independently hydrogen, C1-6alkyl, -CH2CH2OH; or
NR R1 taken together form pyrrolidine, piperidine, 4-hydroxypiperidine, piperazine, 4-methylpiperazine, morpholine, or thiomo holine; and
Ar1 is thiophene or phenyl substituted with 0-3 substituents selected from halogen, Cι_6alkyl, Cι-6alkoxy, cyano, trifluoromethyl, aceto, CO2R5, and CONR9R10;
or a pharmaceutically acceptable salt or solvate of these compounds.
2. A compound of claim 1 where X is O. R502C
3. A compound of claim 2 where A-L is R5O2CCH2CH2CH2, '" ■ ' , or
Figure imgf000080_0001
4. A compound of claim 2 where Het is pyrazole, imidazole, oxazole, or triazole.
5. A compound of claim 4 where Formula (I) is Formula (la).
Figure imgf000080_0002
(la)
6. A compound of claim 4 where Formula (I) is Formula (lb).
Figure imgf000081_0001
(lb) 7. A compound of claim 4 where Formula (I) is Formula (Ic).
Figure imgf000081_0002
(Ic)
A compound of claim 4 where Formula (I) is Formula (Id).
Figure imgf000081_0003
(Id)
9. A compound of claim 4 where Formula (I) is Formula (le).
Figure imgf000081_0004
(le)
10. A compound of claim 2 where R is cyclohexyl.
11. A compound of claim 2 where R is
Figure imgf000081_0005
12. A compound of claim 2 selected from the group consisting of (1) ethyl l-cyclohexyl-5-(4-benzyloxyphenyl)-lH-pyrazole-3-carboxylate;
(2) methyl (2E)-3-{5-[4-(benzyloxy)phenyl]-l-cyclohexyl-lH-pyrazol-3-yl}-2- propenoate;
(3) (2E)-3-[l-cyclohexyl-5-(4-benzyloxyphenyl)-lH-pyrazol-3-yl]-2-propenoic acid;
(4) methyl (2Ε)-3-(5-{4-[(4'-chloro-4-methoxy-l,l'-biphenyl-2- yl)methoxy]phenyl}-l-cyclohexyl-lΗ-pyrazol-3-yl)-2-propenoate;
(5) (2E)-3-(5-{4-[(4'-chloro-4-methoxy-l,l'-biphenyl-2-yl)methoxy]phenyl}-l- cyclohexyl-lH-pyrazol-3-yl)-2-propenoic acid;
(6) ethyl (2E)-3-{5-[4-(benzyloxy)phenyl]-l-cyclohexyl-lH-pyrazol-3-yl}-2- methyl-2-propenoate;
(7) (2E)-3-{5-[4-(benzyloxy)phenyl]-l-cyclohexyl-lH-pyrazol-3-yl}-2-methyl-2- propenoic acid;
(8) ethyl (2E)-3-(5-{4-[(4'-chloro-4-methoxy-l,l'-biphenyl-2- yl)methoxy]phenyl } - 1 -cyclohexyl- lH-pyrazol-3-yl)-2-methyl-2-propenoate;
(9) (2E)-3-(5- {4-[(4'-chloro-4-methoxy- 1 , 1 '-biphenyl-2-yl)methoxy]phenyl } - 1 - cyclohexyl-lH-pyrazol-3-yl)-2-methyl-2-propenoic acid;
(10) ethyl (2E)-3-(5-{4-[(t-butyl-2-bromo-5-phenylcarboxylate)methoxy]phenyl}- l-cyclohexyl-lH-pyrazol-3-yl)-2-methyl-2-propenoate;
(11) ethyl (2E)-3-(5-{4-[(4'-chloro-4-t-butoxycarbonyl-l,l'-biphenyl-2- yl)methoxy]phenyl}-l-cyclohexyl-lH-pyrazol-3-yl)-2-methyl-2-propenoate; ( 12) ethyl (2E)-3-(5- { 4-[(4'-Chloro-4-N-methylcarbamoyl- 1 , 1 '-biphenyl-2- yl)methoxy]phenyl}-l-cyclohexyl-lH-pyrazol-3-yl)-2-methyl-2-propenoate;
(13) (2E)-3-(5-{4-[(4'-Chloro-4-N-methylcarbamoyl-l,r-biphenyl-2- yl)methoxy]phenyl } - 1 -cyclohexyl- lH-pyrazol-3-yl)-2-methyl-2-propenoic acid;
(14) methyl 3- { 5- [4-(benzyloxy)phenyl] - 1 -cyclohexyl- 1 H-pyrazol-3- yl}propanoate;
(15) 3-{5-[4-(benzyloxy)phenyl]-l-cyclohexyl-lH-pyrazol-3-yl}propanoic acid;
( 16) methyl 3-(5- { 4-[(4'-chloro-4-methoxy- 1 , 1 '-biphenyl-2-yl)methoxy]phenyl } - 1- cyclohexyl-lH-pyrazol-3-yl)propanoate;
(17) 3-(5- { 4-[(4'-chloro-4-methoxy- 1 , 1 '-biphenyl-2-yl)methoxy]phenyl } - 1 - cyclohexyl- lH-pyrazol-3-yl)ρropanoic acid;
(18) ethyl 3- { 5-[4-(benzyloxy)phenyl]- 1 -cyclohexyl- lH-pyrazol-3-yl } -2- propynoate;
(19) 3- { 5-[4-(benzyloxy)phenyl]- 1 -cyclohexyl- lH-pyrazol-3-yl } -2-propynoic acid;
(20) ethyl 3-(5- { 4- [(4'-chloro-4-methoxy- 1 , 1 '-biphenyl-2-yl)methoxy]phenyl } - 1 - cyclohexyl- lH-pyrazol-3-yl)-2-propynoate;
(21) 3-(5- { 4-[(4'-chloro-4-methoxy- 1 , 1 '-biphenyl-2-yl)methoxy]phenyl } - 1 - cyclohexyl- lH-pyrazol-3-yl)-2-propynoic acid;
(22) ethyl 5-[4-(benzyloxy)phenyl]-4-bromo-l-cyclohexyl-lH-pyrazole-3- carboxylate; (23) methyl (2E)-3-{5-[4-(benzyloxy)phenyl]-4-bromo-l-cyclohexyl-lH-pyrazol- 3-yl}-2-propenoate;
(24) (2E)-3-{5-[4-(benzyloxy)phenyl]-4-bromo-l-cyclohexyl-lH-pyrazol-3-yl}-2- propenoic acid;
(25) methyl (2E)-3-{5-[4-(benzyloxy)phenyl]-l-cyclohexyl-4-phenyl-lH-pyrazol- 3-yl}-2-propenate;
(26) (2E)-3-{5-[4-(benzyloxy)ρhenyl]-l-cyclohexyl-4-ρhenyl-lH-pyrazol-3-yl}-2- propenoic acid;
(27) methyl (2E)-3-(5-{ 4-[4' -chloro-4-methoxy- 1 , 1 -biphenyl)methoxy] phenyl } - 1 - cyclohexyl-4-phenyl-5-lH-pyrazol-3-yl]-2-propenoate;
(28) (2E)-3-(5- { 4- [4' -chloro-4-methoxy- 1 , 1 -biphenyl)methoxy]phenyl } - 1 - cyclohexyl-4-phenyl-5-lH-pyrazol-3-yl]-2-propenoic acid;
(29) ethyl l-[4-(benzyloxy)phenyl]-5-cyclohexyl-lH-pyrazole-3-carboxylate;
(30) methyl (2E)-3-{ l-[4-(benzyloxy)phenyl]-5-cyclohexyl-lH-pyrazol-3-yl}-2- propenoate;
(31) (2E)-3-{ l-[4-(benzyloxy)phenyl]-5-cyclohexyl-lH-pyrazol-3-yl}-2-propenoic acid;
(32) methyl (2E)-3-(l-{4-[(4'-chloro-4-methoxy-l,l'-biphenyl-2- yl)methoxy]phenyl}-5-cyclohexyl-lH-pyrazol-3-yl)-2-propenoate;
(33) (2E)-3-( 1 - { 4- [(4'-chloro-4-methoxy- 1 , 1 '-biphenyl-2-yl)methoxy]phenyl } -5- cyclohexyl-lH-pyrazol-3-yl)-2-propenoic acid; (34) ethyl (2E)-3-{ l-[4-(benzyloxy)phenyl]-5-cyclohexyl-lH-pyrazol-3-yl}-2- methyl-2-propenoate;
(35) (2E)-3-{ l-[4-(benzyloxy)phenyl]-5-cyclohexyl-lH-pyrazol-3-yl}-2-methyl-2- propenoic acid;
(36) ethyl (2E)-3-(l-{4-[(4'-chloro-4-methoxy-l,l'-biphenyl-2- yl)methoxy]phenyl}-5-cyclohexyl-lH-pyrazol-3-yl)-2-methyl-2-propenoate;
(37) (2E)-3-( 1 - { 4-[(4'-chloro-4-methoxy- 1 , 1 '-biphenyl-2-yl)methoxy]phenyl } -5- cyclohexyl- lH-pyrazol-3-yl)-2-methyl-2-propenoic acid;
(38) ethyl (2Ε)-l-[4-(4 ' -Chloro-4-methylcarbamoyl-biρhenyl-2-ylmethoxy)- phenyl]-5-cyclohexyl-lΗ-pyrazol-3-yl]-2-methyl-2-propenoate;
(39) (2E)-l-[4-(4'-chloro-4-methylcarbamoyl-biphenyl-2-ylmethoxy)-phenyl]-5- cyclohexyl-lH-pyrazol-3-yl]-2-methyl-2-propenoic acid;
(40) methyl 3-{ l-[4-(benzyloxy)phenyl]-5-cyclohexyl-lH-pyrazol-3- yljpropanoate;
(41) 3-{ l-[4-(benzyloxy)phenyl]-5-cyclohexyl-lH-pyrazol-3-yl}propenoic acid;
(42) methyl 3-( 1 - { 4-[(4'-chloro-4-methoxy- 1 , 1 '-biphenyl-2-yl)methoxy]phenyl } -5- cyclohexyl-lH-pyrazol-3-yl)propanoate;
(43) 3-( 1 - { 4- [(4'-chloro-4-methoxy- 1 , 1 '-biphenyl-2-yl)methoxy]phenyl } -5- cyclohexyl-lH-pyrazol-3-yl)propanoic acid;
(44) tert-butyl (2E)-3-{2-[4-(benzyloxy)phenyl]-l-cyclohexyl-lH-imidazol-4-yl }- 2-propenoate; (45) (2E)-3-{2-[4-(benzyloxy)phenyl]-l-cyclohexyl-lH-imidazol-4-yl}-2- propenoic acid;
(46) methyl (2E)-3- { 2-[4-(benzyloxy)phenyl]- 1 -cyclohexyl- lH-imidazol-4-yl } -2- propenoate;
(47) methyl (2Ε)-3-(2-{4-[(4'-chloro-4-methoxy-l,l'-biphenyl-2- yl)methoxy] phenyl } - 1 -cyclohexyl- 1 Η-imidazol-4-yl)-2-propenoate;
(48) (2E)-3-(2- { 4-[(4'-chloro-4-methoxy- 1 , 1 '-biphenyl-2-yl)methoxy]phenyl } - 1 - cyclohexyl- lH-imidazol-4-yl)-2-propenoic acid;
(49) ethyl (2E)-3- { 2- [4-(benzyloxy)phenyl] - 1 -cyclohexyl- 1 H-imidazol-4-yl } -2- methyl-2-propenoate;
(50) (2Ε)-3 - { 2- [4-(benzyloxy)phenyl] - 1 -cyclohexyl- 1 Η-imidazol-4-yl } -2-methyl- 2-propenoic acid;
(51) ethyl (2E)-3-(2-{4-[(4'-chloro-4-methoxy-l,l'-biphenyl-2- yl)methoxy]phenyl } - 1 -cyclohexyl- lH-imidazol-4-yl)-2-methyl-2-propenoate;
(52) (2E)-3-(2- { 4- [(4'-chloro-4-methoxy- 1 , 1 '-biphenyl-2-yl)methoxy]phenyl } - 1 - cyclohexyl-lH-imidazol-4-yl)-2-methyl-2-propenoic acid;
(53) t-Butoxy (2E)-3-(2-{4-[(4'-chloro-4-N-methylcarbamoyl-l,l'-biphenyl-2- yl)methoxy] phenyl } - 1 -cyclohexyl- 1 H-imidazol-4-yl)-2-methyl-2-propenoate; and
(54) (2E)-3-(2- {4-[(4'-chloro-4-N-methylcarbamoyl- 1 , 1 '-biphenyl-2- yl)methoxy]phenyl } - 1 -cyclohexyl- 1 H-imidazol-4-yl)-2-methyl-2-propenoic acid.
13. A pharmaceutical composition comprising a therapeutic amount of a compound of claim 1 , or its pharmaceutically acceptable salt or solvate, and a pharmaceutically acceptable carrier.
14. The pharmaceutical composition of claim 13 further comprising a therapeutic amount of
(a) a replicase inhibitor, (b) a metalloprotease inhibitor,
(c) a NS3 protease inhibitor,
(d) a NS3 helicase inhibitor,
(e) a NS5A inhibitor,
(f) a NS5B polymerase inhibitor, (g) or a combination of the above.
15. A method for inhibiting hepatitis C NS5B RNA-dependent RNA polymerase in a patient in need of such treatment comprising administering a therapeutic amount of a compound of claim 1 to a patient in need of such treatment.
16. The method of claim 15 further comprising co-administering a therapeutic amount of
(a) a replicase inhibitor, (b) a metalloprotease inhibitor,
(c) a NS3 protease inhibitor,
(d) a NS3 helicase inhibitor,
(e) a NS5A inhibitor,
(f) a NS5B polymerase inhibitor, (g) or a combination of the above.
17. A method for treating or preventing a hepatitis C viral infection in a patient in need of such treatment comprising administering a therapeutic amount of a compound of claim 1 to a patient in need of such treatment.
18. The method of claim 17 further comprising co-administering a therapeutic amount of (a) a replicase inhibitor,
(b) a metalloprotease inhibitor,
(c) a NS3 protease inhibitor,
(d) a NS3 helicase inhibitor, (e) a NS5A inhibitor,
(f) a NS5B polymerase inhibitor,
(g) or a combination of the above.
19. A method for treating or preventing hepatitis C in a patient in need of such treatment comprising administering a therapeutic amount of a compound of claim 1 to a patient in need of such treatment.
20. The method of claim 19 further comprising co-administering a therapeutic amount of
(a) a replicase inhibitor,
(b) a metalloprotease inhibitor,
(c) a NS3 protease inhibitor,
(d) a NS3 helicase inhibitor, (e) a NS5A inhibitor,
(f) a NS5B polymerase inhibitor,
(g) or a combination of the above.
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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AT502258B1 (en) * 2005-07-22 2007-09-15 Univ Wien COX-I-INHIBITOR COMPOUNDS
EP2054388A1 (en) * 2006-08-17 2009-05-06 Boehringer Ingelheim International GmbH Viral polymerase inhibitors
WO2009076747A1 (en) 2007-12-19 2009-06-25 Boehringer Ingelheim International Gmbh Viral polymerase inhibitors
WO2010080874A1 (en) 2009-01-07 2010-07-15 Scynexis, Inc. Cyclosporine derivative for use in the treatment of hcv and hiv infection

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070032488A1 (en) * 2005-08-05 2007-02-08 Genelabs Technologies, Inc. 6-Membered aryl and heteroaryl derivatives for treating viruses
WO2008106167A1 (en) * 2007-02-28 2008-09-04 Conatus Pharmaceuticals, Inc. Combination therapy comprising matrix metalloproteinase inhibitors and caspase inhibitors for the treatment of liver diseases
EP2144604B1 (en) 2007-02-28 2011-09-21 Conatus Pharmaceuticals, Inc. Methods for the treatment of chronic viral hepatitis C using RO 113-0830
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US20110158908A1 (en) 2007-08-24 2011-06-30 Sapporo Medical University Cyclosporin a-binding protein
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US10201584B1 (en) 2011-05-17 2019-02-12 Abbvie Inc. Compositions and methods for treating HCV
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CN112055713A (en) 2018-03-01 2020-12-08 安娜普尔纳生物股份有限公司 Compounds and compositions for treating diseases associated with APJ receptor activity

Citations (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000006529A1 (en) 1998-07-27 2000-02-10 Istituto Di Ricerche Di Biologia Molecolare P Angeletti S.P.A. Diketoacid-derivatives as inhibitors of polymerases
WO2000010573A1 (en) 1998-08-21 2000-03-02 Viropharma Incorporated Compounds, compositions and methods for treating or preventing viral infections and associated diseases
WO2000013708A1 (en) 1998-09-04 2000-03-16 Viropharma Incorporated Methods for treating or preventing viral infections and associated diseases
WO2000018231A1 (en) 1998-09-25 2000-04-06 Viropharma Incorporated Methods for treating or preventing viral infections and associated diseases
WO2001032153A2 (en) 1999-11-04 2001-05-10 Shire Biochem Inc. Method for the treatment or prevention of flaviviridae viral infection using nucleoside analogues
WO2001047883A1 (en) 1999-12-27 2001-07-05 Japan Tobacco Inc. Fused-ring compounds and use thereof as drugs
WO2002004425A2 (en) 2000-07-06 2002-01-17 Boehringer Ingelheim (Canada) Ltd. Viral polymerase inhibitors
EP1256628A2 (en) 2001-05-10 2002-11-13 Agouron Pharmaceuticals, Inc. Hepatitis c virus (hcv) ns5b rna polymerase and mutants thereof
WO2002100851A2 (en) 2001-06-11 2002-12-19 Shire Biochem Inc. Thiophene derivatives as antiviral agents for flavivirus infection
WO2003007945A1 (en) 2001-07-20 2003-01-30 Boehringer Ingelheim (Canada) Ltd. Viral polymerase inhibitors
WO2003010141A2 (en) 2001-07-25 2003-02-06 Boehringer Ingelheim (Canada) Ltd. Hepatitis c virus polymerase inhibitors with a heterobicyclic structure
US20030050320A1 (en) 1999-12-27 2003-03-13 Japan Tobacco Inc. Fused-ring compounds and use thereof as drugs
WO2003026587A2 (en) 2001-09-26 2003-04-03 Bristol-Myers Squibb Company Compounds useful for treating hepatitus c virus

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0859642A2 (en) * 1995-10-17 1998-08-26 G.D. Searle & Co. Method of detecting cyclooxygenase-2
AU1324300A (en) * 1998-10-26 2000-05-15 Vertex Pharmaceuticals Incorporated Pentacyclic compounds useful as inhibitors of hepatitis c virus ns3 helicase
AR035543A1 (en) * 2001-06-26 2004-06-16 Japan Tobacco Inc THERAPEUTIC AGENT FOR HEPATITIS C THAT INCLUDES A CONDENSED RING COMPOUND, CONDENSED RING COMPOUND, PHARMACEUTICAL COMPOSITION THAT UNDERSTANDS, BENZIMIDAZOL, THIAZOL AND BIFENYL COMPOUNDS USED AS INTERMEDIARY COMPARTMENTS OF COMPARTMENTS

Patent Citations (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000006529A1 (en) 1998-07-27 2000-02-10 Istituto Di Ricerche Di Biologia Molecolare P Angeletti S.P.A. Diketoacid-derivatives as inhibitors of polymerases
WO2000010573A1 (en) 1998-08-21 2000-03-02 Viropharma Incorporated Compounds, compositions and methods for treating or preventing viral infections and associated diseases
WO2000013708A1 (en) 1998-09-04 2000-03-16 Viropharma Incorporated Methods for treating or preventing viral infections and associated diseases
WO2000018231A1 (en) 1998-09-25 2000-04-06 Viropharma Incorporated Methods for treating or preventing viral infections and associated diseases
WO2001032153A2 (en) 1999-11-04 2001-05-10 Shire Biochem Inc. Method for the treatment or prevention of flaviviridae viral infection using nucleoside analogues
US20030050320A1 (en) 1999-12-27 2003-03-13 Japan Tobacco Inc. Fused-ring compounds and use thereof as drugs
WO2001047883A1 (en) 1999-12-27 2001-07-05 Japan Tobacco Inc. Fused-ring compounds and use thereof as drugs
WO2002004425A2 (en) 2000-07-06 2002-01-17 Boehringer Ingelheim (Canada) Ltd. Viral polymerase inhibitors
EP1256628A2 (en) 2001-05-10 2002-11-13 Agouron Pharmaceuticals, Inc. Hepatitis c virus (hcv) ns5b rna polymerase and mutants thereof
WO2002100851A2 (en) 2001-06-11 2002-12-19 Shire Biochem Inc. Thiophene derivatives as antiviral agents for flavivirus infection
WO2003007945A1 (en) 2001-07-20 2003-01-30 Boehringer Ingelheim (Canada) Ltd. Viral polymerase inhibitors
WO2003010141A2 (en) 2001-07-25 2003-02-06 Boehringer Ingelheim (Canada) Ltd. Hepatitis c virus polymerase inhibitors with a heterobicyclic structure
WO2003026587A2 (en) 2001-09-26 2003-04-03 Bristol-Myers Squibb Company Compounds useful for treating hepatitus c virus

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AT502258B1 (en) * 2005-07-22 2007-09-15 Univ Wien COX-I-INHIBITOR COMPOUNDS
EP2054388A1 (en) * 2006-08-17 2009-05-06 Boehringer Ingelheim International GmbH Viral polymerase inhibitors
EP2054388A4 (en) * 2006-08-17 2009-10-28 Boehringer Ingelheim Int Viral polymerase inhibitors
WO2009076747A1 (en) 2007-12-19 2009-06-25 Boehringer Ingelheim International Gmbh Viral polymerase inhibitors
WO2010080874A1 (en) 2009-01-07 2010-07-15 Scynexis, Inc. Cyclosporine derivative for use in the treatment of hcv and hiv infection

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ATE498398T1 (en) 2011-03-15
EP1663105B1 (en) 2011-02-16
JP2007505114A (en) 2007-03-08
WO2005034850A3 (en) 2006-03-16
ES2358904T3 (en) 2011-05-16
US7112601B2 (en) 2006-09-26
US20050075376A1 (en) 2005-04-07
EP1663105A2 (en) 2006-06-07
EP1663105A4 (en) 2009-04-15
JP4897484B2 (en) 2012-03-14

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