WO2005012550A3 - Screening methods and libraries of trace amounts of dna from uncultivated microorganisms - Google Patents

Screening methods and libraries of trace amounts of dna from uncultivated microorganisms Download PDF

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Publication number
WO2005012550A3
WO2005012550A3 PCT/US2004/024954 US2004024954W WO2005012550A3 WO 2005012550 A3 WO2005012550 A3 WO 2005012550A3 US 2004024954 W US2004024954 W US 2004024954W WO 2005012550 A3 WO2005012550 A3 WO 2005012550A3
Authority
WO
WIPO (PCT)
Prior art keywords
dna
trace amounts
libraries
species
screening methods
Prior art date
Application number
PCT/US2004/024954
Other languages
French (fr)
Other versions
WO2005012550A2 (en
Inventor
Jay Short
Original Assignee
Diversa Corp
Keller Martin
Wyborski Denise
Chang Hwai
Abulencia Carl
Jay Short
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Diversa Corp, Keller Martin, Wyborski Denise, Chang Hwai, Abulencia Carl, Jay Short filed Critical Diversa Corp
Publication of WO2005012550A2 publication Critical patent/WO2005012550A2/en
Publication of WO2005012550A3 publication Critical patent/WO2005012550A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/136—Screening for pharmacological compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Abstract

The invention provides methods for making a gene library from trace amounts of DNA derived from a plurality of species of organisms comprising obtaining trace amounts of cDNA, gDNA, or genomic DNA fragments from a plurality of species of organisms, amplifying the DNA so obtained, and ligating the DNA to a DNA vector to generate a library of constructs in which genes are contained in the DNA. The invention also provides methods for screening clones having DNA recovered from trace amounts of DNA derived from a plurality of species of uncultivated organisms. The invention also provides methods for identifying and enriching for a polynucleotide encoding an activity of interest.
PCT/US2004/024954 2003-07-31 2004-07-30 Screening methods and libraries of trace amounts of dna from uncultivated microorganisms WO2005012550A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US63324803A 2003-07-31 2003-07-31
US10/633,248 2003-07-31
US57347304P 2004-05-21 2004-05-21
US60/573,473 2004-05-21

Publications (2)

Publication Number Publication Date
WO2005012550A2 WO2005012550A2 (en) 2005-02-10
WO2005012550A3 true WO2005012550A3 (en) 2007-08-23

Family

ID=34119186

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/024954 WO2005012550A2 (en) 2003-07-31 2004-07-30 Screening methods and libraries of trace amounts of dna from uncultivated microorganisms

Country Status (1)

Country Link
WO (1) WO2005012550A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006111784A1 (en) * 2005-04-20 2006-10-26 Parco Tecnologico Padano Srl Method for enlarging the range of applicability of multiple displacement amplification of linear dna

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5783431A (en) * 1996-04-24 1998-07-21 Chromaxome Corporation Methods for generating and screening novel metabolic pathways
US5958672A (en) * 1995-07-18 1999-09-28 Diversa Corporation Protein activity screening of clones having DNA from uncultivated microorganisms
US6054267A (en) * 1995-12-07 2000-04-25 Diversa Corporation Method for screening for enzyme activity
US6124120A (en) * 1997-10-08 2000-09-26 Yale University Multiple displacement amplification
US20010034031A1 (en) * 1997-06-16 2001-10-25 Recombinant Biocatalysis Inc. Delaware Corporation High throughput screening for novel enzymes
US20030118998A1 (en) * 2001-10-15 2003-06-26 Dean Frank B. Nucleic acid amplification

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5958672A (en) * 1995-07-18 1999-09-28 Diversa Corporation Protein activity screening of clones having DNA from uncultivated microorganisms
US6054267A (en) * 1995-12-07 2000-04-25 Diversa Corporation Method for screening for enzyme activity
US5783431A (en) * 1996-04-24 1998-07-21 Chromaxome Corporation Methods for generating and screening novel metabolic pathways
US20010034031A1 (en) * 1997-06-16 2001-10-25 Recombinant Biocatalysis Inc. Delaware Corporation High throughput screening for novel enzymes
US6124120A (en) * 1997-10-08 2000-09-26 Yale University Multiple displacement amplification
US20030118998A1 (en) * 2001-10-15 2003-06-26 Dean Frank B. Nucleic acid amplification

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
ROHWER ET AL.: "Production of shotgun libraries using random amplification", BIOTECHNIQUES, vol. 31, July 2001 (2001-07-01), pages 108 - 118 *

Also Published As

Publication number Publication date
WO2005012550A2 (en) 2005-02-10

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