WO2005008235A3 - Electrophoretic system with multi-notch filter and laser excitation source - Google Patents

Electrophoretic system with multi-notch filter and laser excitation source Download PDF

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Publication number
WO2005008235A3
WO2005008235A3 PCT/US2004/022202 US2004022202W WO2005008235A3 WO 2005008235 A3 WO2005008235 A3 WO 2005008235A3 US 2004022202 W US2004022202 W US 2004022202W WO 2005008235 A3 WO2005008235 A3 WO 2005008235A3
Authority
WO
WIPO (PCT)
Prior art keywords
detector
notch filter
charge
excitation source
laser excitation
Prior art date
Application number
PCT/US2004/022202
Other languages
French (fr)
Other versions
WO2005008235A2 (en
Inventor
Mark F Oldham
Eric S Nordman
Richard T Reel
John S Di Shigeura
Original Assignee
Applera Corp
Mark F Oldham
Eric S Nordman
Richard T Reel
Shigeura Janice G Lf
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Applera Corp, Mark F Oldham, Eric S Nordman, Richard T Reel, Shigeura Janice G Lf filed Critical Applera Corp
Publication of WO2005008235A2 publication Critical patent/WO2005008235A2/en
Publication of WO2005008235A3 publication Critical patent/WO2005008235A3/en

Links

Classifications

    • G01N15/1433—
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D57/00—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C
    • B01D57/02—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C by electrophoresis
    • G01N15/149—
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1434—Electro-optical investigation, e.g. flow cytometers using an analyser being characterised by its optical arrangement
    • G01N2015/144—Imaging characterised by its optical setup
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means

Abstract

Apparatus and methods for detecting analytes in a sample are provided. The apparatus includes: one or more channels having a dectection zone (14); one or more irradiation sources (16) disposed for irradiating the detection zone (14) with radiation; a detector (34) capable of collecting at least one chage corresponding to an emission beam (22) emitted from the detection zone (14), the detector (34) having an output; and a time delay integration system coupled to the detector for effecting time delay integration of the at least one charge by accumulating the at least one charge before reading the at least one charge at the output of the detector. Various embodiments provide an apparatus with a multi-notch filter. Various embodiments provide a solid state laser, a micro-wire laser, or an organic light-emitting diode as a irradiation source.
PCT/US2004/022202 2003-07-08 2004-07-08 Electrophoretic system with multi-notch filter and laser excitation source WO2005008235A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US48546803P 2003-07-08 2003-07-08
US60/485,468 2003-07-08
US48611203P 2003-07-10 2003-07-10
US60/486,112 2003-07-10

Publications (2)

Publication Number Publication Date
WO2005008235A2 WO2005008235A2 (en) 2005-01-27
WO2005008235A3 true WO2005008235A3 (en) 2005-08-18

Family

ID=34083344

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/US2004/022203 WO2005008221A1 (en) 2003-07-08 2004-07-08 Time-delay integration in a flow cytometry system
PCT/US2004/022202 WO2005008235A2 (en) 2003-07-08 2004-07-08 Electrophoretic system with multi-notch filter and laser excitation source

Family Applications Before (1)

Application Number Title Priority Date Filing Date
PCT/US2004/022203 WO2005008221A1 (en) 2003-07-08 2004-07-08 Time-delay integration in a flow cytometry system

Country Status (1)

Country Link
WO (2) WO2005008221A1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7355696B2 (en) * 2005-02-01 2008-04-08 Arryx, Inc Method and apparatus for sorting cells
CN103176279B (en) * 2011-12-22 2015-08-26 维林光电(苏州)有限公司 Same light path collimating apparatus and alignment method thereof
US10697884B2 (en) 2014-12-04 2020-06-30 Chemometec A/S Image cytometer implementation

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5141609A (en) * 1990-11-16 1992-08-25 The Trustees Of The Leland Stanford Junior University Method and device employing time-delayed integration for detecting sample components after separation
WO1999050916A1 (en) * 1998-04-01 1999-10-07 Massachusetts Institute Of Technology Quantum dot white and colored light emitting diodes
US6256148B1 (en) * 1998-05-19 2001-07-03 Lockheed Martin Corporation Rugate filter and method of making same
US6381025B1 (en) * 1999-08-19 2002-04-30 Texas Tech University Interferometric detection system and method
US6464852B1 (en) * 1998-12-03 2002-10-15 State University Of New York At Stony Brook Multicapillary bundle for electrophoresis and detection for DNA
US6485625B1 (en) * 1995-05-09 2002-11-26 Curagen Corporation Apparatus and method for the generation, separation, detection, and recognition of biopolymer fragments
WO2003010524A1 (en) * 2001-07-25 2003-02-06 Applera Corporation Time-delay integration in electrophoretic detection systems

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20010006416A1 (en) * 1999-01-11 2001-07-05 Johnson Paul E. Ribbon flow cytometry apparatus and methods
US6507391B2 (en) * 2000-08-25 2003-01-14 Amnis Corporation Measuring the velocity of small moving objects such as cells
EP1334347A1 (en) * 2000-09-15 2003-08-13 California Institute Of Technology Microfabricated crossflow devices and methods

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5141609A (en) * 1990-11-16 1992-08-25 The Trustees Of The Leland Stanford Junior University Method and device employing time-delayed integration for detecting sample components after separation
US6485625B1 (en) * 1995-05-09 2002-11-26 Curagen Corporation Apparatus and method for the generation, separation, detection, and recognition of biopolymer fragments
WO1999050916A1 (en) * 1998-04-01 1999-10-07 Massachusetts Institute Of Technology Quantum dot white and colored light emitting diodes
US6256148B1 (en) * 1998-05-19 2001-07-03 Lockheed Martin Corporation Rugate filter and method of making same
US6464852B1 (en) * 1998-12-03 2002-10-15 State University Of New York At Stony Brook Multicapillary bundle for electrophoresis and detection for DNA
US6381025B1 (en) * 1999-08-19 2002-04-30 Texas Tech University Interferometric detection system and method
WO2003010524A1 (en) * 2001-07-25 2003-02-06 Applera Corporation Time-delay integration in electrophoretic detection systems

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
SWEEDLER J V ET AL: "FLUORESCENCE DETECTION IN CAPILLARY ZONE ELECTROPHORESIS USING A CHARGE-COUPLED DEVICE WITH TIME-DELAYED INTEGRATION", ANALYTICAL CHEMISTRY, AMERICAN CHEMICAL SOCIETY. COLUMBUS, US, vol. 63, no. 5, 1 March 1991 (1991-03-01), pages 496 - 502, XP000207134, ISSN: 0003-2700 *

Also Published As

Publication number Publication date
WO2005008221A1 (en) 2005-01-27
WO2005008235A2 (en) 2005-01-27

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