WO2005002551A2

WO2005002551A2 - Vanilloid receptor (vr) 1 inhibitors for treatment of human immunodeficiency virus (hiv)-mediated pain states

Info

Publication number: WO2005002551A2
Application number: PCT/EP2004/006679
Authority: WO
Inventors: Axel Bouchon; Keiko Misawa
Original assignee: Bayer Healthcare Ag
Priority date: 2003-07-03
Filing date: 2004-06-21
Publication date: 2005-01-13
Also published as: WO2005002551A3; EP1493438A1

Abstract

The invention relates to the application of Vanilloid receptor (VR) 1 inhibitors for drug development and for the treatment of HIV-mediated neuropathies and neuropathic pain states. Further, the inventor identified a novel signaling cascade connecting the HIV receptor CXCR4 to VR1. Thus, the invention provides molecular evidence that HIV-mediated pain states - initiated upon binding of the virus to CXCR4 - can be inhibited by VR1 antagonists blocking the final execution of the CXCR4/VR1 pathway. In addition, the invention demonstrates that present standard therapies for HIV-mediated pain (which do not include VR1 inhibitors) can not interfere with the CXCR4/VR1 pathway thus explaining inefficient patient treatment in the clinics.

Description

Vanilloid receptor (VR) 1 inhibitors for treatment of

Human Immunodeficiency Virus (UlWmediated pain states

1. BACKGROUND OF THE INVENTION

The Acquired Immune Deficiency Syndrome (AIDS) is characterized by susceptibility to infection with opportunistic pathogens, the occurrence of an aggressive form of Human Herpes Virus (HHV)-8-mediated Kaposi's sarcoma or B cell lymphoma, accompanied by a profound decrease in the number of CD4⁺ T cells [Janeway and Travers (1997)]. The Human Immunodeficiency Virus (HIV) is the causative agent of AIDS [Gelmann et al. (1983); Gallo et al. (1983); Barre-Sinoussi (1983)]. HIV is an enveloped retrovirus. Each virus particle has two copies of an RNA genome, which is transcribed into DNA upon infection of target cells and subsequently integrated in the host genome. The RNA transcripts serve both as mRNA to direct the synthesis of novel viruses and later as the genome of newly built virus particles. HIV enters target cells by high-affinity binding of viral envelope protein gpl20 to the cellular surface protein CD4. Fusion and entry of the virus depends on the presence of G-protein coupled receptors (GPCRs), such as CCR5 or CXCR4, that acts as cofactors. Physiologically, CCR5 and CXCR4 are stimulated by interaction with the chemokines RANTES, MlP-lβ and SDF-l , respectively [Bleul et al. (1996); Oberlin et al. (1996); Doranz (1996); Deng et al. (1996); Dragic et al. (1996)]. CCR5 and CXCR4 are G_rProtein coupled receptors that upon stimulation induce various signal transduction pathways [Stantchev and Broder (2001)], in particular mobilization of intracellular Ca²⁺ stores, modulation of Adenylyl Cyclase (AC) activity, induction of Mitogen-activated protein kinases (MAPK) and Phospholipase A 2 (PLA₂) [Audubert et al. (1999); Zhu et al. (1999)]. CCR5 is particularly expressed by macro- phages and primary T cells, whereas CXCR4 is primarily expressed on mature CD4⁺ T helper (T_H) cells. Early during infection, CD4⁺/CCR5⁺ macrophages are the central target for HIV, whereas later in infection, the viral phenotype switches to a CD4⁺ T_H-trophic type. This transformation is followed by a rapid decline of CD4⁺ T cell counts and progression of AIDS [Janeway and Travers (1997)].

In addition to cancer and opportunistic diseases, AIDS patients display a variety of neurological symptoms. These include allodynia, primary and secondary hyperalgesia and different types of sensory and motor neuropathies [Brinley et al. (2001); Brannagan et al.(1997); Hewitt et al. (1997); Griffin et al. (1998); Bouhassira et al. (1999)]. The peripheral neuropathies associated with HIV-1 infection are a diverse group including acute and chronic inflammatory demyelinating poly- neuropathy, mononeuropathy multiplex, diffuse infiltrative lymphocytosis syndrome with neuropathy, progressive polyneurophathy, CMN- and HZN-related neuropathies and in particular distal sensory painful polyneuropathy (DSP). DSP becomes symptomatic in very early and later stages of HIV infection and closely correlates to decreased CD4⁺ T cell numbers, increased HIV-load and gpl20 concentration in the plasma and the nervous system (NS). On cellular level, DSP is characterized by (i) chronic stimulation and activation of peripheral nociceptive neurons; (ii) neuronal cell death due to pathologically prolonged Ca²⁺ influx from the extracellular space; and (iii) infiltration of activated, inflammatory cells to the site of neuronal destruction [Pardo et al. (2001); Kaul et al. (2001)]. The virus does not infect and replicate in peripheral nerve fibers and DRG neurons which is consistent with the absence of CD4 [Kaul et al. (2001)]. Thus, HlV-mediated nociceptive and neurotoxic effects do not require neuronal infection. However several data suggest a direct neuronal activation by HIV or gpl20 [Kaul et al. (2001); Brinley et al. (2001)]. Recently, neuronal CCR5 and CXCR4 expression has been demonstrated, particularly on primary nociceptive neurons and dorsal root ganglion (DRG) neurons [Miller and Meucci (1999)]. Although DRG neurons do not express CD4, it has been previously shown, that gpl20 can bind to CXCR4 in a CD4-independent manner [Hesselgesser et al. (1997)]. Upon binding, gpl20 excites DRG neurons leading to Ca²⁺ influx, depolarization and the release of nociceptive peptides, such as Substance P [Oh et al. (2001)] and CGRP. Chronic neuronal stimulation by HIV induces Ca²⁺-mediated neuronal apoptosis, necrosis or parapoptosis [Kaul et al. (2001); Leist and Jaattela (2001); Hesselgesser et al. (1997)]. Thus, it has been suggested that gpl20-mediated acute neuronal activation and gpl20-associated neurotoxic effects utilizes identical signal transduction pathways [Kaul et al. (2001); Leist and Jaattela (2001)]. In vivo, peripheral [Eron et al. (1996); Oh et al. (2001)] or central [Milligan et al. (2000)] administration of gpl20 induces tactile allodynia and thermal hyperalgesia suggesting that CXCR4 is indeed involved in pain perception. In addition, Herzberg and Sagen demonstrated that epineural exposure to gpl20 induces neuropathic pain and damage to the gpl20-exposed sciatic nerve [Herzberg and Sagen (2001)]. However, a molecular link between HlV-mediated neuronal stimulation, neurotoxicity, neuropathy and neuropathic pain as not been described so far.

HTV-mediated neuropathic pain is currently treated according to the WHO analgetic ladder for treatment of cancer pain. Standard analgetic drugs include Capsaicin, non-steroidal anti- inflammatory drugs (NSAIDs), tricyclic antidepressants, anticonvulsants, mild and strong opoids [Brinley et al. (2001); Glare (2001); Verma (2001); Paice et al. (2000)]. However, although targeting different peripheral and central pain pathways, the therapeutic effects in the clinics have been dissatisfying with below 15% reduction in HlV-mediated neuropathic pain [Brinley et al. (2001)]. Few clinical trails have been conducted so far to prove the efficiency of the currently applied drugs in HlV-mediated neuropathic pain. Remarkably, Amitriptyline and Mexiletine, two drugs frequently used for efficient treatment of cancer pain and diabetic neuropathies, are completely ineffective in clinical trails investigating HlV-mediated neuropathic pain [Shlay et al. (1998); Kieburtz et al. (1998)]. In addition, it is anecdotally reported that Opoids and in particular NSAIDs are less effective in HIN-mediated neuropathic pain compared to other types of neuropathic pain, such as cancer-mediated and diabetes-associated neuropathic pain [Glare (2001); Verma (2001)]. A clinical trail investigating the analgesic effect of ΝSAEDs and Opoids in HlV-mediated neuropathic pain has not been conducted yet. Further, topically applied Capsaicin has been found to be highly effective in relieving pain associated with various neuropathic pain syndromes, and was mentioned as a possible topical adjuvant analgesic for the relief of DSP in HIV patients [Paice et al. (2000)]. Two possible mechanisms for Capsaicin-mediated pain release are proposed. Firstly, Capsaicin induces acute nociception by stimulating Vanilloid Receptor (VR) 1 [Caterina et al. (1997); Tomi- naga et al. (1998)] and subsequently desensitizing and downregulating VRl in vitro [Bhave et al. (2002)]. VRl downmodulation reduces the algesic effects of endogenous VRl stimuli leading to pain relief. However, in the presence of VRl phosphorylation - an event frequently occurring during chronic pain - Capsaicin-mediated downmodulation is blocked. Under these conditions Capsaicin may act synergistically with endogenous VRl stimuli resulting in pain amplification [Bhave et al. (2002)]. Secondly, Capsaicin inhibits ΝF-κB activation in inflammatory immune cells in a VRl -independent fashion and is a potent anti-inflammatory compound in vivo [(Sancho et al. (2002); Kim et al. (2003)]. Surprisingly, a randomized, double-masked clinical trail demonstrated, that Capsaicin-treatment of HIV patients with chronic DSP leads to strongly increased pain rather than pain relief [Paice et al. (2000)]. Thus, VRl may be involved or even directly stimulated during HIV-mediated neuropathic pain. In conclusion, these reports show that standard drugs do not specifically target HIN-activated pain pathways and in addition delineate the high medical need for an efficient treatment of HIV-mediated neuropathic pain.

VRl (Ace. No. AJ272063 (human), AF029310 (rat)) is a non-specific cation channel with preference for Ca²⁺ expressed preferentially in small sensory neurons and is activated by heat, low pH, phosphorylation and certain ligands. VRl activation leads to massive Ca²⁺ influx from the extracellular space followed by neuronal depolarization, release of algesic neuropeptides (CGRP and Substance P) and activation of downstream signaling cascades. Chronic stimulation of VRl induces Ca²⁺-mediated neuronal cell death [Hiura et al. (2002); Jambrina et al. (2003)]. The quarter- nary structure of VRl is predominated by homotetramers enabling the cooperative interaction of all stimuli-sensitive and ligand-binding sites [Kedei et al. (2001); Kuzhikandathil et al. (2001); Hui et al. (2003)]. VRl is considered a molecular sensor that detects various painful stimuli [Caterina et al. (1997); Tominaga et al. (1998)]. Indeed, experiments performed with the VRl antagonist Capsazepin [Kelly et al. (2002); Walker et al. (2003)] and with VRl -deficient mice [Caterina et al. (2000); Davis et al. (2000)] demonstrated that the receptor is essential for inflammatory pain. Initially, VRl has been described as receptor for exogenous substances such as Capsaicin and Res- iniferatoxin ("Vanilloids") [Caterina et al. (1997)]. In addition, it has been recently demonstrated that metabolic products ("Endovanilloids") of the PLA₂/12-lipoxygenase (LOX)-pathway of ara- chidonic acid (AA) metabolism can activate VRl. Endovanilloids, such as 12- hydroperoxyeicosatetraenoic acid (12-HPETE), are structurally similar to Capsaicin, and interact with the Capsaicin-binding site [Hwang et al. (2000); Shin et al. (2002)]. Thus, stimuli that induce the production of AA in small sensory neurons lead to the synthesis of endovanilloids responsible for VRl activation, subsequent neuronal depolarization, neuropeptide release and nociception. The present invention provides the finding, that antagonists of VRl and of members of the VRl signaling pathway can be used as pharmaceutical drugs for the treatment of HIV-mediated pain states.

2. DETAILED DESCRIPTION OF THE INVENTION

The invention provides mechanistical evidence that gpl20/HIV-receptor CXCR4 and VRl are linked by a novel signal transduction pathway in primary neurons. Upon stimulation with gpl20 the pathway subsequently utilizes CXCR4, ERK and p38, PLA2 and 12-LOX leading to the production of endovanilloids. The endovanilloids, 12-HPETE and 12-HETE stimulate VRl leading to Ca²⁺ influx and release of algesic peptides, such as CGRP. Gpl20-mediated CGRP-release can be completely blocked with VRl antagonists further delineating the role of NR1 for the execution of gpl20-induced neuronal stimulation. The pathway is widely independent of other nociceptive signal transduction pathways. Thus, the invention provides (i) a mechanism for HIV-mediated neuropathic pain; (ii) a plausible and likely mechanism for HlV-induced neurotoxicity; (iii) an explanation for the low efficacy of Opoids, anticonvulsants, antidepressants, and ΝSAIDs, in particular COX inhibitors, in the treatment of HIV-mediated neuropathic pain; and (iv) an explanation for the enhanced pain perception in DSP patients treated with Capsaicin. The invention particularly relates to the use of VRl inhibitors for drug development and for the treatment of HIV-mediated neuropathy and neuropathic pain. Due to the absence of a satisfying pain therapy, HIV-mediated neuropathic pain is an area displaying the highest medical need.

The invention relates to methods of screening for VRl antagonists useful for the treatment of HIN- mediated neuropathy or HIV-mediated pain.

A further embodiment of the invention are methods of screening for VRl antagonists useful for the treatment of HIN-mediated neuropathy or HIN-mediated pain using Ca²⁺-sensitive dyes of biolu- minescence assays in cell-free or cellular assay systems.

Furthermore, the invention relates to the use of a VRl antagonist for the preparation of a pharmaceutical composition for the treatment of HIV-mediated neuropathy or HIN-mediated pain. The invention also relates to the use of a VRl antagonist for the preparation of a pharmaceutical composition for the treatment of HIV-mediated neuropathy or HIV-mediated pain, wherein HIV- mediated neuropathy or pain is caused by a disorder comprised in a group of disorders consisting of acute and chronic inflammatory demyelinating polyneuropathy, diffuse infϊltrative lymphocyto- sis syndrome with neuropathy, mononeuropathy multiplex, progressive polyneurophathy, Cy- tomegalovirus (CMV)-related neuropathies, Herpes zoster virus (HZN)-related neuropathies, distal sensory painful polyneuropathy (DSP), and HIV-associated dementia.

Furthermore, the invention relates to the use of a NRl antagonist for the preparation of a pharmaceutical composition for the treatment of HIN-mediated neuropathy or HIV-mediated pain, wherein the VRl antagonist is compound 1.

The invention also relates to the use of compound 1 for the preparation of a pharmaceutical composition for the treatment of HIV-mediated neuropathy or HIV-mediated pain, wherein HIV- mediated neuropathy or pain is caused by a disorder comprised in a group of disorders consisting of acute and chronic inflammatory demyelinating polyneuropathy, diffuse infiltrative lymphocyto- sis syndrome with neuropathy, mononeuropathy multiplex, progressive polyneurophathy, Cy- tomegalovirus (CMV)-related neuropathies, Herpes zoster virus (HZN)-related neuropathies, distal sensory painful polyneuropathy (DSP), and HIV-associated dementia.

A further embodiment of the invention is a pharmaceutical composition containing a VRl antagonist for the treatment of HIN-mediated neuropathy or HIN-mediated pain.

The invention also relates to a pharmaceutical composition containing a NRl antagonist for the treatment of HIV-mediated neuropathy or HIV-mediated pain, wherein HIV-mediated neuropathy or pain is caused by a disorder comprised in a group of disorders consisting of acute and chronic inflammatory demyelinating polyneuropathy, diffuse infϊltrative lymphocytosis syndrome with neuropathy, mononeuropathy multiplex, progressive polyneurophathy, Cytomegalovirus (CMV)- related neuropathies, Herpes zoster virus (HZV)-related neuropathies, distal sensory painful polyneuropathy (DSP), and HrV-associated dementia.

The invention also relates to a pharmaceutical composition containing a VRl antagonist for the treatment of HIV-mediated neuropathy or HIV-mediated pain where the active ingredient is the compound 1.

Furthermore, the invention relates to a pharmaceutical composition containing a VRl antagonist for the treatment of HIV-mediated neuropathy or HIV-mediated pain where the active ingredient is the compound 1, wherein HIV-mediated neuropathy or pain is caused by a disorder comprised in a group of disorders consisting of acute and chronic inflammatory demyelinating polyneuropathy, diffuse infiltrative lymphocytosis syndrome with neuropathy, mononeuropathy multiplex, progressive polyneurophathy, Cytomegalovirus (CMV)-related neuropathies, Herpes zoster virus (HZV)- related neuropathies, distal sensory painful polyneuropathy (DSP), and HIV-associated dementia.

Another embodiment of the invention is the use of a VRl antagonist for the regulation of VRl activity in a subject having HIV-mediated neuropathy or HTV-mediated pain.

Furthermore, the invention relates to the use of a VRl antagonist for the regulation of VRl activity in a subject having HIV-mediated neuropathy or HIV-mediated pain, wherein HIN-mediated neuropathy or pain is caused by a disorder comprised in a group of disorders consisting of acute and chronic inflammatory demyelinating polyneuropathy, diffuse infϊltrative lymphocytosis syndrome with neuropathy, mononeuropathy multiplex, progressive polyneurophathy, Cytomegalovirus (CMN)-related neuropathies, Herpes zoster virus (HZN)-related neuropathies, distal sensory painful polyneuropathy (DSP), and HIN-associated dementia.

Another embodiment of the invention are methods of screening for 12-LOX antagonists useful for the treatment of HIV-mediated neuropathy or HIV-mediated pain.

Furthermore, the invention relates to the use of a 12-LOX antagonist for the preparation of a pharmaceutical composition for the treatment of HIV-mediated neuropathy or HIN-mediated pain.

The invention also relates to the use of a 12-LOX antagonist for the preparation of a pharmaceutical composition for the treatment of HIV-mediated neuropathy or HIV-mediated pain, wherein HIV-mediated neuropathy or pain is caused by a disorder comprised in a group of disorders consisting of acute and chronic inflammatory demyelinating polyneuropathy, diffuse infϊltrative lymphocytosis syndrome with neuropathy, mononeuropathy multiplex, progressive polyneurophathy, Cytomegalovirus (CMV)-related neuropathies, Herpes zoster virus (HZN)-related neuropathies, distal sensory painful polyneuropathy (DSP), and HIN-associated dementia.

A further embodiment of the invention is a pharmaceutical composition containing a 12-LOX antagonist for the treatment of HIV-mediated neuropathy or HIV-mediated pain.

The invention also relates to a pharmaceutical composition containing a 12-LOX antagonist for the treatment of HIV-mediated neuropathy or HIN-mediated pain, wherein HIN-mediated neuropathy or pain is caused by a disorder comprised in a group of disorders consisting of acute and chronic inflammatory demyelinating polyneuropathy, diffuse infiltrative lymphocytosis syndrome with neuropathy, mononeuropathy multiplex, progressive polyneurophathy, Cytomegalovirus (CMN)- related neuropathies, Herpes zoster virus (HZV)-related neuropathies, distal sensory painful polyneuropathy (DSP), and HrV-associated dementia.

Another embodiment of the invention is the use of a 12-LOX antagonist for the regulation of 12- Lox activity in a subject having HIV-mediated neuropathy or HIV-mediated pain.

Furthermore, the invention relates to the use of a 12-LOX antagonist for the regulation of 12-LOX activity in a subject having HIV-mediated neuropathy or HIV-mediated pain, wherein HIV- mediated neuropathy or pain is caused by a disorder comprised in a group of disorders consisting of acute and chronic inflammatory demyelinating polyneuropathy, diffuse infiltrative lymphocytosis syndrome with neuropathy, mononeuropathy multiplex, progressive polyneurophathy, Cytomegalovirus (CMV)-related neuropathies, Herpes zoster virus (HZV)-related neuropathies, distal sensory painful polyneuropathy (DSP), and HIV-associated dementia.

Another embodiment of the invention are methods of screening for PLA₂ antagonists useful for the treatment of HIV-mediated neuropathy or HIV-mediated pain.

A further embodiment of the invention is the use of a PLA₂ antagonist for the preparation of a pharmaceutical composition for the treatment of HIV-mediated neuropathy or HIV-mediated pain.

Furthermore, the invention relates to the use of a PLA₂ antagonist for the preparation of a pharmaceutical composition for the treatment of HIV-mediated neuropathy or HIN-mediated pain, wherein HIN-mediated neuropathy or pain is caused by a disorder comprised in a group of disorders consisting of acute and chronic inflammatory demyelinating polyneuropathy, diffuse infiltrative lymphocytosis syndrome with neuropathy, mononeuropathy multiplex, progressive polyneurophathy, Cytomegalovirus (CMN)-related neuropathies, Herpes zoster virus (HZN)-related neuropathies, distal sensory painful polyneuropathy (DSP), and HIN-associated dementia.

A further embodiment of the invention is a pharmaceutical composition containing a PLA₂ antagonist for the treatment of HIV-mediated neuropathy or HIV-mediated pain.

The invention also relates to a pharmaceutical composition containing a PLA₂ antagonist for the treatment of HIV-mediated neuropathy or HIV-mediated pain, wherein HIV-mediated neuropathy or pain is caused by a disorder comprised in a group of disorders consisting of acute and chronic inflammatory demyelinating polyneuropathy, diffuse infiltrative lymphocytosis syndrome with neuropathy, mononeuropathy multiplex, progressive polyneurophathy, Cytomegalovirus (CMV)- related neuropathies, Herpes zoster virus (HZV)-related neuropathies, distal sensory painful polyneuropathy (DSP), and HrV-associated dementia. Another embodiment of the invention is the use of a PLA₂ antagonist for the regulation of PLA₂ activity in a subject having HTV-mediated neuropathy or HTV-mediated pain.

Furthermore, the invention relates to the use of a PLA₂ antagonist for the regulation of PLA₂ activity in a subject having HTV-mediated neuropathy or HTV-mediated pain, wherein HTV-mediated neuropathy or pain is caused by a disorder comprised in a group of disorders consisting of acute and chronic inflammatory demyelinating polyneuropathy, diffuse infϊltrative lymphocytosis syndrome with neuropathy, mononeuropathy multiplex, progressive polyneurophathy, Cytomegalovirus (CMV)-related neuropathies, Herpes zoster virus (HZV)-related neuropathies, distal sensory painful polyneuropathy (DSP), and HTV-associated dementia.

Another embodiment of the invention are methods of screening for MAPK antagonists useful for the treatment of HTV-mediated neuropathy or HTV-mediated pain.

Furthermore, the invention relates to the use of a MAPK antagonist for the preparation of a pharmaceutical composition for the treatment of HTV-mediated neuropathy or HTV-mediated pain.

The invention also relates to the use of a MAPK antagonist for the preparation of a pharmaceutical composition for the treatment of HTV-mediated neuropathy or HIN-mediated pain, wherein HIN- mediated neuropathy or pain is caused by a disorder comprised in a group of disorders consisting of acute and chronic inflammatory demyelinating polyneuropathy, diffuse infϊltrative lymphocytosis syndrome with neuropathy, mononeuropathy multiplex, progressive polyneurophathy, Cytomegalovirus (CMN)-related neuropathies, Herpes zoster virus (HZN)-related neuropathies, distal sensory painful polyneuropathy (DSP), and HTV-associated dementia.

A further embodiment of the invention is a pharmaceutical composition containing a MAPK antagonist for the treatment of HIN-mediated neuropathy or HIN-mediated pain.

The invention also relates to a pharmaceutical composition containing a MAPK antagonist for the treatment of HTV-mediated neuropathy or HTV-mediated pain, wherein HTV-mediated neuropathy or pain is caused by a disorder comprised in a group of disorders consisting of acute and chronic inflammatory demyelinating polyneuropathy, diffuse infiltrative lymphocytosis syndrome with neuropathy, mononeuropathy multiplex, progressive polyneurophathy, Cytomegalovirus (CMN)- related neuropathies, Herpes zoster virus (HZN)-related neuropathies, distal sensory painful polyneuropathy (DSP), and HIN-associated dementia.

Another embodiment of the invention is the use of a MAPK antagonist for the regulation of MAPK activity in a subject having HTV-mediated neuropathy or HIN-mediated pain. Furthermore, the invention relates to the use of a MAPK antagonist for the regulation of MAPK activity in a subject having HTV-mediated neuropathy or HTV-mediated pain, wherein HTV- mediated neuropathy or pain is caused by a disorder comprised in a group of disorders consisting of acute and chronic inflammatory demyelinating polyneuropathy, diffuse infiltrative lymphocytosis syndrome with neuropathy, mononeuropathy multiplex, progressive polyneurophathy, Cytomegalovirus (CMN)-related neuropathies, Herpes zoster virus (HZV)-related neuropathies, distal sensory painful polyneuropathy (DSP), and HTV-associated dementia.

Another embodiment of the invention are methods of screening for CXCR4 antagonists useful for the treatment of HTV-mediated neuropathy or HIN-mediated pain.

Furthermore, the invention relates to the use of a CXCR4 antagonist for the preparation of a pharmaceutical composition for the treatment of HTV-mediated neuropathy or HTV-mediated pain.

The invention also relates to the use of a CXCR4 antagonist for the preparation of a pharmaceutical composition for the treatment of HTV-mediated neuropathy or HIN-mediated pain, wherein HTV-mediated neuropathy or pain is caused by a disorder comprised in a group of disorders consisting of acute and chronic inflammatory demyelinating polyneuropathy, diffuse infiltrative lymphocytosis syndrome with neuropathy, mononeuropathy multiplex, progressive polyneurophathy, Cytomegalovirus (CMV)-related neuropathies, Herpes zoster virus (HZV)-related neuropathies, distal sensory painful polyneuropathy (DSP), and HTV-associated dementia.

A further embodiment of the invention is a pharmaceutical composition containing a CXCR4 antagonist for the treatment of HIV-mediated neuropathy or HIN-mediated pain.

The invention also relates to a pharmaceutical composition containing a CXCR4 antagonist for the treatment of HIV-mediated neuropathy or HTV-mediated pain, wherein HTV-mediated neuropathy or pain is caused by a disorder comprised in a group of disorders consisting of acute and chronic inflammatory demyelinating polyneuropathy, diffuse infϊltrative lymphocytosis syndrome with neuropathy, mononeuropathy multiplex, progressive polyneurophathy, Cytomegalovirus (CMV)- related neuropathies, Herpes zoster virus (HZN)-related neuropathies, distal sensory painful polyneuropathy (DSP), and HTV-associated dementia.

Another embodiment of the invention is the use of a CXCR4 antagonist for the regulation of CXCR4 activity in a subject having HTV-mediated neuropathy or HTV-mediated pain.

Furthermore, the invention relates to the use of a CXCR4 antagonist for the regulation of CXCR4 activity in a subject having HTV-mediated neuropathy or HTV-mediated pain, wherein HTV- mediated neuropathy or pain is caused by a disorder comprised in a group of disorders consisting of acute and chronic inflammatory demyelinating polyneuropathy, diffuse infiltrative lymphocytosis syndrome with neuropathy, mononeuropathy multiplex, progressive polyneurophathy, Cytomegalovirus (CMV)-related neuropathies, Herpes zoster virus (HZN)-related neuropathies, distal sensory painful polyneuropathy (DSP), and HIN-associated dementia.

Another embodiment of the invention are methods of screening for a modulator interfering upstream of CXCR4 ligands useful for the treatment of HTV-mediated neuropathy or HTV-mediated pain.

Furthermore, the invention relates to the use of a modulator interfering upstream of CXCR4 ligands for the preparation of a pharmaceutical composition for the treatment of HIV-mediated neuropathy or HTV-mediated pain.

The invention also relates to the use of a modulator interfering upstream of CXCR4 ligands for the preparation of a pharmaceutical composition for the treatment of HIN-mediated neuropathy or HTV-mediated pain, wherein HTV-mediated neuropathy or pain is caused by a disorder comprised in a group of disorders consisting of acute and chronic inflammatory demyelinating polyneuropathy, diffuse infiltrative lymphocytosis syndrome with neuropathy, mononeuropathy multiplex, progressive polyneurophathy, Cytomegalovirus (CMV)-related neuropathies, Herpes zoster virus (HZV)-related neuropathies, distal sensory painful polyneuropathy (DSP), and HTV-associated dementia.

A further embodiment of the invention is a pharmaceutical composition containing a modulator interfering upstream of CXCR4 ligands for the treatment of HTV-mediated neuropathy or HIN- mediated pain.

The invention also relates to a pharmaceutical composition containing an antagonist interfering upstream of CXCR4 ligands for the treatment of HIN-mediated neuropathy or HIN-mediated pain, wherein HTV-mediated neuropathy or pain is caused by a disorder comprised in a group of disorders consisting of acute and chronic inflammatory demyelinating polyneuropathy, diffuse infϊltrative lymphocytosis syndrome with neuropathy, mononeuropathy multiplex, progressive polyneurophathy, Cytomegalovirus (CMV)-related neuropathies, Herpes zoster virus (HZN)-related neuropathies, distal sensory painful polyneuropathy (DSP), and HTV-associated dementia.

Antagonists within the meaning of the invention include but are not limited to proteins, nucleic acids, carbohydrates, antibodies, small molecules, or any other molecule which acts as an antagonist or modulator on the proteins disclosed. 2J Pharmaceutical Compositions

The antagonists or modulators within the meaning the invention can be incorporated into pharmaceutical compositions suitable for administration of a therapeutic dose of the antagonist or modulator to a mammal or human. Such compositions typically comprise the antagonist or modulator and a pharmaceutically acceptable carrier. As used herein the language "pharmaceutically acceptable carrier" is intended to include any and all solvents, dispersion media, coatings, antibacterial and antifungal agents, isotonic and absorption delaying agents, and the like, compatible with pharmaceutical administration. The use of such media and agents for pharmaceutically active substances is well known in the art. Except insofar as any conventional media or agent is incompatible with the active compound, use thereof in the compositions is contemplated. Supplementary active compounds can also be incorporated into the compositions.

The compositions may be administered alone or in combination with at least one other agent, such as a stabilizing compound, which may be administered in any sterile, biocompatible pharmaceutical carrier including, but not limited to, saline, buffered saline, dextrose, and water. The compositions may be administered to a patient alone, or in combination with other agents, drugs or hormones.

A pharmaceutical composition of the invention is formulated to be compatible with its intended route of administration. Examples of routes of administration include parenteral, e.g., intravenous, intradermal, subcutaneous, oral, transdermal (topical), transmucosal, and rectal administration. Solutions or suspensions used for parenteral, intradermal, or subcutaneous application can include the following components: a sterile diluent such as water for injection, saline solution, fixed oils, polyethylene glycols, glycerine, propylene glycol or other synthetic solvents; antibacterial agents such as benzyl alcohol or methyl parabens; antioxidants such as ascorbic acid or sodium bisulfite; chelating agents such as ethylenediaminetetraacetic acid; buffers such as acetates, citrates or phosphates and agents for the adjustment of tonicity such as sodium chloride or dextrose. pH can be adjusted with acids or bases, such as hydrochloric acid or sodium hydroxide. The parenteral preparation can be enclosed in ampoules, disposable syringes or multiple dose vials made of glass or plastic.

Pharmaceutical compositions suitable for injectable use include sterile aqueous solutions (where water soluble) or dispersions and sterile powders for the extemporaneous preparation of sterile injectable solutions or dispersions. For intravenous administration, suitable carriers include physiological saline, bacteriostatic water, Cremophor EM™ (BASF, Parsippany, NJ) or phosphate buffered saline (PBS). In all cases, the composition must be sterile and should be fluid to the extent that easy syringability exists. It must be stable under the conditions of manufacture and storage and must be preserved against the contaminating action of microorganisms such as bacteria and fungi. The carrier can be a solvent or dispersion medium containing, for example, water, ethanol, a pharmaceutically acceptable polyol like glycerol, propylene glycol, liquid polyetheylene glycol, and suitable mixtures thereof. The proper fluidity can be maintained, for example, by the use of a coating such as lecithin, by the maintenance of the required particle size in the case of dispersion and by the use of surfactants. Prevention of the action of microorganisms can be achieved by various antibacterial and antifungal agents, for example, parabens, chlorobutanol, phenol, ascorbic acid, thimerosal, and the like. Tn many cases, it will be preferable to include iso- tonic agents, for example, sugars, polyalcohols such as mannitol, sorbitol, sodium chloride in the composition. Prolonged absorption of the injectable compositions can be brought about by including in the composition an agent which delays absorption, for example, aluminum monostearate and gelatin. Sterile injectable solutions can be prepared by incorporating the active compound (e.g., a polypeptide or antibody) in the required amount in an appropriate solvent with one or a combination of ingredients enumerated above, as required, followed by filtered sterilisation. Generally, dispersions are prepared by incorporating the active compound into a sterile vehicle which contains a basic dispersion medium and the required other ingredients from those enumerated above. In the case of sterile powders for the preparation of sterile injectable solutions, the preferred methods of preparation are vacuum drying and freeze-drying which yields a powder of the active ingredient plus any additional desired ingredient from a previously sterile-filtered solution thereof.

Oral compositions generally include an inert diluent or an edible carrier. They can be enclosed in gelatine capsules or compressed into tablets. For the purpose of oral therapeutic administration, the active compound can be incorporated with excipients and used in the form of tablets, troches, or capsules. Oral compositions can also be prepared using a fluid carrier for use as a mouthwash, wherein the compound in the fluid carrier is applied orally and swished and expectorated or swallowed.

Pharmaceutically compatible binding agents, and/or adjuvant materials can be included as part of the composition. The tablets, pills, capsules, troches and the like can contain any of the following ingredients, or compounds of a similar nature: a binder such as microcrystalline cellulose, gum tragacanth or gelatin; an excipient such as starch or lactose, a disintegrating agent such as alginic acid, Primogel, or corn starch; a lubricant such as magnesium stearate or sterotes; a glidant such as colloidal silicon dioxide; a sweetening agent such as sucrose or saccharin; or a flavoring agent such as peppermint, methyl salicylate, or orange flavoring. For administration by inhalation, the compounds are delivered in the form of an aerosol spray from a pressurized container or dispenser which contains a suitable propellant, e.g., a gas such as carbon dioxide, or a nebulizer.

Systemic administration can also be by transmucosal or transdermal means. For transmucosal or transdermal administration, penetrants appropriate to the barrier to be permeated are used in the formulation. Such penetrants are generally known in the art, and include, for example, for transmucosal administration, detergents, bile salts, and fusidic acid derivatives. Transmucosal administration can be accomplished through the use of nasal sprays or suppositories. For transdermal administration, the active compounds are formulated into ointments, salves, gels, or creams as generally known in the art.

The compounds can also be prepared in the form of suppositories (e.g., with conventional suppository bases such as cocoa butter and other glycerides) or retention enemas for rectal delivery.

In one embodiment, the active compounds are prepared with carriers that will protect the compound against rapid elimination from the body, such as a controlled release formulation, including implants and microencapsulated delivery systems. Biodegradable, biocompatible polymers can be used, such as ethylene vinyl acetate, polyanhydrides, polyglycolic acid, collagen, polyorthoesters, and polylactic acid. Methods for the preparation of such formulations will be apparent to those skilled in the art. The materials can also be obtained commercially from Alza Corporation and Nova Pharmaceuticals, Inc. Liposomal suspensions (including liposomes targeted to infected cells with monoclonal antibodies to viral antigens) can also be used as pharmaceutically acceptable carriers. These can be prepared according to methods known to those skilled in the art, for example, as described in U.S. 4,522,811.

It is especially advantageous to formulate oral or parenteral compositions in dosage unit form for ease of administration and uniformity of dosage. Dosage unit form as used herein refers to physically discrete units suited as unitary dosages for the subject to be treated; each unit containing a predetermined quantity of active compound calculated to produce the desired therapeutic effect in association with the required pharmaceutical carrier. The specification for the dosage unit forms of the invention are dictated by and directly dependent on the unique characteristics of the active compound and the particular therapeutic effect to be achieved, and the limitations inherent in the art of compounding such an active compound for the treatment of individuals.

The pharmaceutical compositions can be included in a container, pack, or dispenser together with instructions for administration. For pharmaceutical compositions which include an antagonist of VRl, a compound which reduces VRl expression, or a compound which reduces expression or activity of a protein in the VRl signaling pathway or any combination thereof, the instructions for administration will specify use of the composition for HTV-mediated pain states.

2.2. Determination of a Therapeutically Effective Dose

The determination of a therapeutically effective dose is well within the capability of those skilled in the art. For any compound, the therapeutically effective dose can be estimated initially either in cell culture assays or in animal models, usually rats, rabbits, dogs, or pigs. The animal model also can be used to determine the appropriate concentration range and route of administration. Such information can then be used to determine useful doses and routes for administration in humans.

Therapeutic efficacy and toxicity, e.g., ED₅₀ (the dose therapeutically effective in 50% of the population) and LD₅₀ (the dose lethal to 50% of the population), can be determined by standard pharmaceutical procedures in cell cultures or experimental animals. The dose ratio of toxic to therapeutic effects is the therapeutic index, and it can be expressed as the ratio, LD₅₀/ED₅o. Pharmaceutical compositions which exhibit large therapeutic indices are preferred. The data obtained from cell culture assays and animal studies is used in formulating a range of dosage for human use. The dosage contained in such compositions is preferably within a range of circulating concentrations that include the ED₅₀ with little or no toxicity. The dosage varies within this range depending upon the dosage form employed, sensitivity of the patient, and the route of administration. The exact dosage will be determined by the practitioner, in light of factors related to the subject that requires treatment. Dosage and administration are adjusted to provide sufficient levels of the active ingredient or to maintain the desired effect. Factors which can be taken into account include the severity of the disease state, general health of the subject, age, weight, and gender of the subject, diet, time and frequency of administration, drug combination(s), reaction sensitivities, and tolerance/response to therapy. Long-acting pharmaceutical compositions can be administered once or twice daily every 3 to 4 days, every week, or once every two weeks depending on the half-life and clearance rate of the particular formulation.

Normal dosage amounts can vary from 0J micrograms to 100,000 micrograms, up to a total dose of about 1 g, depending upon the route of administration. Guidance as to particular dosages and methods of delivery is provided in the literature and generally available to practitioners in the art.

In any of the embodiments described above, any of the pharmaceutical compositions of the invention can be administered in combination with other appropriate therapeutic agents. Selection of the appropriate agents for use in combination therapy can be made by one of ordinary skill in the art, according to conventional pharmaceutical principles. The combination of therapeutic agents can act synergistically to effect the treatment or prevention of the various disorders described above. Using this approach, one may be able to achieve therapeutic efficacy with lower dosages of each agent, thus reducing the potential for adverse side effects. ^Λ

3. DESCRIPTION OF THE FIGURES

Figure 1: CGRP-release from primary rat DRG neurons upon stimulation with gpl20, SDF-lα or control peptide.

DRG neurons were isolated from adult male Wistar rats and incubated at 37°C, 5% C0₂ for 36 h at a concentration of 8000 DRG neurons/stimulation point. After washing once with RAB buffer, cells were stimulated for 10 min with control peptide (250 pg/ml), gpl20 HTV-I_ΠIB (250 pg/ml) or SDF-lα (1 ng/ml) as indicated. Supernatants were removed and analyzed by EIA for CGRP release.

Figure 2: Kinetics of CGRP-release from isolated rat sciatic nerve upon VRl stimulation or stimulation with gpl20 or SDF-lα.

Sciatic nerve was isolated from adult male Wistar rats, fixed on a polystyrol rod and maintained in RAB buffer until 5 min before stimulation (time point - 5).

(A) RAB buffer was exchanged, the nerve incubated for 5 min and subsequently stimulated (time point 0) with Capsaicin (100 nM in RAB buffer; white diamonds), low pH (RAB buffer pH 4.5; white squares), RAB buffer heated to 55°C (white circles) or RAB buffer pH 1.4 as control (black squares).

(B) RAB buffer was exchanged, the nerve incubated for 5 min and subsequently stimulated (time point 0) with control peptide (250 pg/ml; black squares), SDF-lα (1 ng/ml; white squares) or gpl20 HTV-I_ΠIB (250 pg/ml; grey squares) in RAB buffer pH 7.4.

After 5 min, 10 min and 20 min supernatant was substituted with fresh RAB buffer pH 7.4 (time- point 5 and 10, respectively) and the samples placed on ice until analysis. All samples were analyzed by EIA for CGRP release and normalized by the dry weight of the particular sciatic nerve.

Figure 3: Sensitization of Capsaicin-, low pH- or temperature-induced VRl activation from rat DRG neurons by gpl20.

DRG neurons were isolated from adult male Wistar rats and incubated at 37°C, 5% C0₂ for 36 h at a concentration of 8000 DRG neurons/stimulation point. After washing once with RAB buffer, cells were stimulated for 10 min with control peptide (250 pg/ml; black squares), gpl20 HlV-lπm (250 pg/ml; white squares) or SDF-lα (1 ng/ml; white circles) in the presence of different Capsai- cin concentrations (A), various temperatures (B) and pH values (C). Supernatants were removed and analyzed by EIA for CGRP release.

Figure 4: Inhibition of gpl20- mediated CGRP-release from rat DRG neurons by PD98059, U0126, SB203580, QN, NDGA, Ibuprofen, Capsazepin and compound of example 1.

DRG neurons were isolated from adult male Wistar rats and incubated at 37°C, 5% C0₂ for 36 h at a concentration of 8000 DRG neurons/stimulation point. After washing once with RAB buffer, cells were stimulated for 10 min with gpl20 H_V-1_Π__B alone (250 pg/ml; black squares) or in the presence of the indicated concentrations of (A) SB203580 (p38 Mi.; white squares), PD98059 (MEK-1 Inh.; black circles) and U0126 (MEK-1 Mi.; white circles); (B) Quinacrine (PLA₂ Mi.; white squares); (C) NDGA (LOX Inh.; white squares); Ibuprofen (COX Inh.; white circles); and (D) Capsazepin (VRl Inh.; white circles) and compound 1 (VRl Inh.; white squares). Supernatants were removed and analyzed by EIA for CGRP release.

Figure 5: Inhibition of gpl20-mediated CGRP release form rat sciatic nerve by Capsazepin and compound of example 1.

RAB buffer was exchanged, the nerve incubated for 5 min and subsequently stimulated for 10 min with gpl20 HTV-I_IΠB (250 pg/ml; black squares) or control peptide (250 pg/ml; black crosses) in the presence of Capsazepin (1 μM; white circles), compound of example 1 (20 nM; white squares) or 0.1% DMSO as control (black squares and crosses). After 5 min, 10 min and 20 min supernatant was substituted with fresh solution or RAB buffer pH 7.4 and the samples placed on ice until analysis. All samples were analyzed by EIA for CGRP release and normalized by the dry weight of the particular sciatic nerve.

4. EXAMPLES

The present invention is based on the inventors' identification of a novel signaling pathway in primary nociceptive sensory neurons connecting CXCR4, the receptor for SDF-lα and the HTV protein gpl20, with VRl. Thus, NRl antagonists can interfere with SDF-lα-, HTV- and gpl20- mediated neuronal excitation and inhibit neuropathic pain perception, particularly in HTV and AIDS patients. 4.L Example 1

4JJ. Compound 1

N-(7-hydroxy-5,6,7,8-tetrahydro-naphthalen-l-yl)-N'-(4-trifluoromethoxy-benzyl)-urea

This example was performed according to the general Method A (2.2J).

A mixture of 7-hydroxy-5,6,7,8-tetrahydro-naphthalen-l-yl)-carbamic acid phenyl ester (30.0 mg, 0.11 mmol) and 4-trifluoromethoxy-benzylamine (21J mg, 0J 1 mmol) in DMSO (1.0 ml) was stirred at 100°C for 17 hours. The reaction mixture was cooled to room temperature, and water was added. Precipitates were filtered and washed with water then with acetonitrile to obtain N-(7- hydroxy-5,6,7,8-tetrahydro-naphthalen-l-yl)-N'-(4-trifluoromethoxy-benzyl)-urea (6.70 mg, 17 % yield).

^!H NMR (DMSO-ύ?₆) δ 1.54-1.65 (m, 1H), 1.81-1.92 (m, 1H), 2.25-2.38 (m, 1H), 2.68-2.88 (m, 3H), 3.86-3.98 (m, 1H), 4.32 (d, J= 6.0 Hz, 2H), 4.85 (d, J= 4J Hz, 1H), 6.72 (d, J= 1.5 Hz, 1H), 6.98 (t, J= 1.5 Hz, 1H), 7.06 (t, j= 6.0 Hz, 1H), 7.34 (d, J= 8.3 Hz, 2H), 7.43 (d, J= 8.3 Hz, 2H), 7.63 (d, J= 1.5 Hz, 1H), 1.5 (s, 1H).

Molecular weight: 380.36

MS (M+H): 381

mp: 213°C

4J .2. Method A (Synthesis of Compound 1)

The compound (T) can be prepared by (1) reacting 8-amino-l,2,3,4-tefrahydro-naphthalen-2-ol and phenyl chloroformate, and (2) adding amine represented by the formula X-NH₂ to the reaction mixture. The reaction (1) may be carried out in a solvent including, for instance, ethers, such as diox- ane, and tefrahydrofuran; aromatic hydrocarbons such as benzene, toluene and xylene; nifriles such as acetonitrile; amides such as dimethylformamide (DMF) and dimethylacetamide; sulfoxides such as dimethyl sulfoxide, and others.

The reaction temperature can be optionally set depending on the compounds to be reacted. The reaction temperature is usually, but not limited to, about 20°C to 50°C. The reaction may be conducted for, usually, 30 minutes to 10 hours and preferably 1 to 24 hours.

The reaction (2) may be carried out in a solvent including, for instance, ethers, such as dioxane, and tetrahydrofuran; aromatic hydrocarbons such as benzene, toluene and xylene; nifriles such as acetonitrile; amides such as dimethylformamide (DMF) and dimethylacetamide; sulfoxides such as dimethyl sulfoxide, and others.

The reaction temperature can be optionally set depending on the compounds to be reacted. The reaction temperature is usually, but not limited to, about 30°C to 120°C. The reaction may be conducted for, usually, 1 hour to 48 hours and preferably 2 to 24 hours.

The 8-amino-l,2,3,4-tefrahydro-naphthalen-2-ol can be prepared by the use of known techniques, and phenyl chloroformate and amine are commercially available or can be prepared by the use of known techniques.

[Starting compound A]

To a stirred solution of 7-ethoxy-5,8-dihydronaphthalen-l-ylamine (1.07 g, 5.65 mmol) in tetrahydrofuran (30 mL) was added solution of aqueous 2N HC1 (10 mL), and stirred at 40°C for 1 hour. The mixture was neutralized with addition of sodium bicorbonate, and the product was extracted with ethylacetate. The organic layer was washed with water, dried over Na₂S0₄, filtered, and concentrated under reduced pressure to afford 8-amino-3,4-dihydro-lH-naphthalen-2-one (0.71 g, 78 % yield).

MS (ESI) m/z 162 [M+H]⁺

Η NMR (CDCI₃) δ 2.62-2.65 (m, 2H), 3.07 (t, J = 7.25 Hz, 2H), 3J4(s, 2H), 6.65 (d, J = 7.85, 1H), 6.70 (d, J= 1.25 Hz, 1H), 7.07 (t, J= 1.55 Hz, 1H).

Next, to 8-amino-3,4-dihydro-lH-naphthalen-2-one (0.050 g, 0J18 mmol) in methanol (10 mL) was added sodium borohydride (0.030 g, 0J75 mmol) at 0°C, and the mixture was stirred for 1 hour. The mixture was poured into water, and the product was extracted with ethylacetate. The organic layer was dried over Na₂S0₄, filtered, and concentrated under reduced pressure to afford 8- amino-l,2,3,4-tetrahydro-naphthalen-2-ol (0.037 g, 71 % yield).

MS (ESI) m/z 163 [M]⁺

Η NMR (DMSO-Λ5) δ 1.53-1.57 (m, 1H), 1.81-1.85 (m, 1H), 2.16 (dd, J= 7.7 and 16.4 Hz, 1H), 2.61-2.74 (m, 3H), 3.89-3.90 (m, 1H), 4.65 (s, 2H), 4.72 (d, J= 4.1 Hz, 1H), 6.28 (d, J = 7.45 Hz, 1H), 6.28 (d, J= 1.45 Hz, 1H), 6.41 (d, J= 7.7 Hz, 1H), 6.76(t, J= 1.55 Hz, 1H).

4.2. Example 2

4.2.1. Methods of screening for VRl antagonists

4.2J J Fluorescent Ca²⁺ indicators excited with UN light

Fluorescent probes showing a spectral response upon Ca²⁺ binding enables the detection of changes in the intracellular free Ca²⁺ concentration by fluorescence mircoscopy, flow cytometry and fluorescence spectroscopy. The fluorescent, UN-light excitable indicators are mostly derivatives of the Ca²⁺ chelators EGTA, APTRA and BABTA, such as fura-2, indo-1, fura-4F, fura-5F, fura-6F, fura-FF, fura-red, Calcium Green, Oregon Green 488 BABTA. Such indicators can be used for the screening of NRl antagonists using cell-free systems or cellular systems containing primary cells or NRl-transfected cell lines [O'Malley et al. (1999)].

42.12 Fluorescent Ca²⁺ indicators excited with visible light

Fluorescent probes, excitable by visible light, such as fluo-3, fluo-4, Rhod-2, X-Rhod-1 showing a spectral response upon Ca²⁺ binding enables the detection of changes in the intracellular free Ca²⁺ concentration by laser-based instrumentation, such as flowcytometers or laser-scanning microscopes. Such indicators can be used for the screening of VRl antagonists using cell-free systems or cellular systems containing primary cells or VRl-transfected cell lines [O'Malley et al. (1999)].

4.2J.3 Bioluminescent Ca 2+ i:ndicators

Bioluminescence is defined as the production of light by biological organsims which does not require illumination. Transfection of VRl -expressing cells with the photoprotein aequorin form the jellyfish Aequorea victoria can be used for screening of VRl antagonists. The aequorin complex comprises apoaquorin protein, molecular oxygen and the luminophore coelenterazine. When Ca²⁺ binds to the complex, coelenterazine is oxidized to coelenteramide, with a concomiant release of carbon dioxide and blue light. Unlike fluorescent Ca²⁺ indicators, Ca²⁺-bound aequorin can be detected without illuminating the sample. Thus, agonist-induced, NR-1 -mediated Ca²⁺-influx can be detected by increased generation of light, whereas VRl inhibitors antagonize VRl -mediated Ca²⁺- influx and light production [Miller et al. (1994)].

4.3 Example 3

4.3 J gp 120-mediated CGRP-release from DRG neurons and N. Ischadicus

The invention provides evidence that SDF-lα and gpl20 induce release of CGRP from rat DRG neurons and isolated rat sciatic nerves ex vivo (Table 1 and 2; Figure 1 and 2). This is the first evidence that gpl20 can directly stimulate primary peripheral nerves and neurons supporting the hypothesis that direct HTV interaction with neurons is responsible for HTV-associated neuropathies and not secondary effects mediated by HTV-infected immune cells [Kaul et al. (2001)].

Table 1: CGRP-release ([OD₄₀₅]) from rat DRG neurons (23.2. Method B)

Figure 1: CGRP-release from primary rat DRG neurons upon stimulation with gpl20, SDF- lα or control peptide (2J.2. Method B).

DRG neurons were isolated from adult male Wistar rats and incubated at 37°C, 5% C0₂ for 36 h at a concentration of 8000 DRG neurons/stimulation point. After washing once with RAB buffer, cells were stimulated for 10 min with control peptide (250 pg/ml), gpl20 HlV-lms (250 pg/ml) or SDF-lα (1 ng/ml) as indicated. Supernatants were removed and analyzed by EIA for CGRP release.

Table 2A: CGRP-release ([ng CGRP/mg nerve dry weight]; t₀-t₅: stimulation period) from rat sciatic nerve (2.3.3. Method C)

Table 2B: CGRP-release ([ng CGRP/mg nerve dry weight]; t₀-tι₀: stimulation period) from rat sciatic nerve (2.3.3. Method C)

Figure 2: Kinetics of CGRP-release from isolated rat sciatic nerve upon VRl stimulation or stimulation with gpl20 or SDF-lα (2.3.3. Method C).

(C) RAB buffer was exchanged, the nerve incubated for 5 min and subsequently stimulated (time point 0) with Capsaicin (100 nM in RAB buffer; white diamonds), low pH (RAB buffer pH 4.5; white squares), RAB buffer heated to 55°C (white circles) or RAB buffer pH 7.4 as control (black squares).

(D) RAB buffer was exchanged, the nerve incubated for 5 min and subsequently stimulated (time point 0) with control peptide (250 pg/ml; black squares), SDF-lα (1 ng/ml; white squares) or gpl20 HIV- 1 _HE (250 pg/ml; grey squares) in RAB buffer pH 7.4. After 5 min, 10 min and 20 min supernatant was substituted with fresh RAB buffer pH 7.4 (time- point 5 and 10, respectively) and the samples placed on ice until analysis. All samples were analyzed by EIA for CGRP release and normalized by the dry weight of the particular sciatic nerve.

4.3.2 Method B : CGRP-release from rat DRG neurons

4.3.2.1 Preparation of DRG cell culture plates

96-well cell culture plates for cultivation of DRG neurons are coated with Poly-D-Lysin (Gib- coBRL, Grand Island, NY, USA) (50 μg/ml F12 Medium, 50μl/well, 30min at RT, washed 3 x with 200μl sterile water dry completely) and Laminin (GibcoBRL, Grand Island, NY, USA) (5 μg/ml F12 Medium, 30 μl/96-well, 30 min at RT, wash 3 x with 200μl sterile water, dry completely). Upon drying 96well plates can be used directly or be stored at RT for at least 7 days.

4J.2.2 Preparation of rat spinal column and DRGs

Male Wistar rats (180-300 g) are sacrificed by decapitation and bled completely. Upon ventral opening, organs and ribs are removed. In the cranial area the spine is separated from muscle and skin using a scalpel. For caudal separation from the lower lumbar vertebra a pair of bone scissors is used. Further steps of the preparation are performed in ice-cold medium under a clean bench. The spine is divided in three equal pieces and the spine channel is opened first dorsally than ven- trally along the medians leading to six spine fragments. Spinal cord and dura mater are removed with tweezers to grant excess to the capsule of DRGs. Under microscopic control the DRGs can be separated from the peripheral nerve and excess of connecting tissue using a pair of spring scissors and tweezers. Separated DRGs are subsequently transferred to a small dish containing ice-cold medium. Under optimal conditions 2xl0⁶ DRGs/rat can be isolated.

4.3.2.3 Isolation and cultivation of DRG neurons

DRGs are incubated in 4 ml of Collagenase solution for 2 h at 37°C (1.25 mg/ml Collagenase in DMEM (GibcoBRL, Grand Island, NY, USA)), 4 ml Trypsin-EDTA solution for 30 min at 37°C (GibcoBRL, Grand Island, NY, USA)), and DNase solution for 5 min at 37°C (1 mg/ml DNase (GibcoBRL, Grand Island, NY, USA) in DMEM). Cells are finally washed twice in DRG-Medium (Nutrient Mix F12, 10% Horse Serum, 200mM Glucose, 2mM Glutamine (all GibcoBRL, Grand Island, NY, USA)), counted and plated at a concentration of 30000 cells/well. After over night incubation medium containing cell debris and dead cells is substituted.

4.32.4 DRG release assay DRG neurons are gently washed once with Release Assay Buffer (RAB: 138 mM NaCl, 5 mM KCl, 1.2 mM MgCl₂, 2 mM CaCl₂; 10 mM Glucose, 10 mM HEPES pH 7.4; all from Sigma, St. Louis, MO, USA) and stimulated with Capsaicin solution (100 nM Capsaicin in RAB pH 7.4 (Sigma)), RAB pH 4.5 or preheated RAB pH 7.4 (50°C) in the presence or absence of inhibitors. After 5 or 10 min of stimulation samples are neutralized with NaOH if necessary (stimulation with RAB pH 4.5; neutralization: 3.7μl NaOH (200 mM) to llOμl RAB pH 4.5) and the supernatant transferred to EIA plates (CGRP Release Kit, SpiBio, Paris, France). EIA is performed according to the manufacturer's protocol (CGRP Release Kit, SpiBio, Paris, France).

4.3.3 Method C: CGRP-release from rat Nervus Ischadicus

4.3.3.1 Preparation of Nervus ischiadicus (modified after [Sauer et al. (2001)])

Male Wistar rats (180-220 g) are anesthetized using Isofluran, Dinitricoxide and Oxygen (5:68:27 (v/v/v)) in a vaporisator. The hind legs are shaved and opened along the femur. Using tweezers and scissors the Musculus gluteus superficial and Musculus bicepsfemoris are separated gaining access to the Nervus ischiadicus. The Nervus ischiadicus is cut proximally 1 cm before entry into the spine and laterally at the branching of Nervus fibularis and Nervus tibialis. Dependent on the size and weight of the animal, the length of the preparation is between 2 — 3 cm. The nerve is coiled around a polystyrol spatula (Rϋhrspatel, Sarstedt, Germany) and fixed at the ends with cyanacry- late glue (Loctite 401, Loctite GmbH, Germany). The fixed nerve is transferred to a 1.5 ml eppen- dorf tube containing 300 μl RAB, pH 1.4 at RT.

4.3.3.2 Stimulation of Nervus ischiadicus and CGRP-release assay (modified after [Sauer et al. (2001)])

To reset all samples, sciatic nerve preparations are transferred 5 min prior to stimulation to fresh 300 μl of RAB pH 7.4 (prestimulation - point zero). Stimulation is performed for 5 or 10 min with Capsaicin solution (100 nM Capsaicin in RAB pH 7.4), RAB pH 4.5 or preheated RAB pH 7.4 (50°C) in the presence or absence of inhibitors. To evaluate post-stimulation CGRP-release sciatic nerve preparations are transferred in 5-min-intervals to fresh tubes containing RAB pH 1.4. Supernatants of each sample are transferred to EIA plates (CGRP Release Kit, SpiBio, Paris, France) and analyzed in triplicate (3 x lOOμl) according to the manufacterer's protocol. Upon completion of the experiment the sciatic nerve preparations are carefully removed from the spatula, dried on 3MM paper and weighed. CGRP concentrations obtained from the CGRP-release EIA are finally normalized to the dry weight of the sciatic nerve preparations. 4.4 Example 4

The invention provides evidence that gpl20 and SDF-lα can sensitize VRl for stimulation with Capsaicin, low pH and heat (Table 3; Figure 3). Thus gpl20-stimulated pathways directly affect and modify the molecular parameters (EC₅₀, EpH₅₀, ET₅₀) of VRl. Further, these data demonstrate that gpl20-initiated pathways are linked to VRl and operate upstream of VRl activation instead of acting synergistically to VRl -mediated pathways in an parallel manner. The observation that Capsaicin sensitizes for gpl20-mediated CGRP release explains the algesic effect of topical Capsaicin application in HTV patients with DSP [Paice et al. (2000)]. Apparently, in this clinical setting VRl is unable to be desensitized by Capsaicin since neurons are already chronically stimulated downstream of gpl20 [Bhave et al. (2002)]. Thus, Capsaicin amplifies gpl20-mediated algesic effects instead of acting analgesic.

Table 3: EC₅₀ ([mol/1]), ET₅₀ ([°C]) or EρH₅₀ (-log₁₀[H⁺]) in the presence or absence of SDF-lα (1 ng/ml) or gpl20 (250 pg/ml) using CGRP-release from rat DRG neurons (2.32. Method B).

Figure 3: Sensitization of Capsaicin-, low pH- or temperature-induced VRl activation from rat DRG neurons by gpl20 (2.3.2 Method B).

DRG neurons were isolated from adult male Wistar rats and incubated at 37°C, 5% C0₂ for 36 h at a concentration of 8000 DRG neurons/stimulation point. After washing once with RAB buffer, cells were stimulated for 10 min with control peptide (250 pg/ml; black squares), gpl20 HTV-I_ΠIB (250 pg/ml; white squares) or SDF-lα (1 ng/ml; white circles) in the presence of different Capsaicin concentrations (A), various temperatures (B) and pH values (C). Supernatants were removed and analyzed by EIA for CGRP release. 4.5 Example 5

The invention provides data that gpl20-mediated CGRP release from DRG neurons can be completely inhibited by the following specific inhibitors: (i) the MEK-1 inhibitors PD98059 and U0126 (Table 4; Figure 4A); (ii) the p38 inhibitor SB203580 (Tabel 4; Figure 4A) (iii) the PLA₂ inhibitor Quinacrine (QN) (Table 4; Figure 4B); (iv) the 12- and 15-LOX inhibitor Nordihy- droguaiaretic Acid (NDGA) (Table 4; Figure 4C) and (v) the VRl inhibitors Capsazepin (CAZ) (Table 4; Figure 4D) and the Cpd 1 (Table 4; Figure 4D). Thus, the invention clarifies the complete molecular connection from CXCR4 to VRl -stimulation and VR-1 -mediated release of algesic CGRP identifying several potential targets for the intereference with gpl20-mediated neuronal stimulation. Thus, gpl20-mediated neuronal excitation is initiated by interaction with CXCR4 and executed by VRl activation. The data mechanistically explain the observation, that gpl20-induced neuropathy in rats can be antagonized with p38 inhibitors [Kaul et al. (2001)]. Particularly, the inhibition of gpl20-mediated CGRP release with VRl antagonists (Capsazepin and Cpd. 1) opens the possibility to treat HTV-associated neuropathic pain and neuropathies with VRl antagonists. Merfering at the end of the CXCR R1 cascade allows selective inhibition of gpl20-mediated neuronal activation and subsequent neuronal cell death protection sparing other essential functions of the upstream signaling cascade.

Table 4: IC₅₀ ([M]) of indicated inhibitors upon stimulation with gpl20 (250pg/ml) using CGRP-release from rat DRG neurons (2.3.2. Method B).

Figure 4: Inhibition of gpl20- mediated CGRP-release from rat DRG neurons by PD98059, U0126, SB203580, QN, NDGA, Ibuprofen, Capsazepin and Cpd 1 (2J.2. Method B).

DRG neurons were isolated from adult male Wistar rats and incubated at 37°C, 5% C0₂ for 36 h at a concentration of 8000 DRG neurons/stimulation point. After washing once with RAB buffer, cells were stimulated for 10 min with gpl20 HlV-lnm alone (250 pg/ml; black squares) or in the presence of the indicated concentrations of (A) SB203580 (p38 Inh.; white squares), PD98059 (MEK-1 Inh.; black circles) and U0126 (MEK-1 Inh.; white circles); (B) Quinacrine (PLA₂ Inh.; white squares); (C) NDGA (LOX Inh.; white squares); Ibuprofen (COX Inh.; white circles); and (D) Capsazepin (VRl Inh.; white circles) and Cpd 1 (VRl Inh.; white squares). Supernatants were removed and analyzed by EIA for CGRP release.

4.6 Example 6

CAZ and Cpd 1 are capable to block CGRP release from gpl20-stimulated sciatic nerve (Table 5; Figure 5) delineating that VRl antagonists block gpl20-mediated neuronal activation. The data further demonstrate that VRl antagonists can completely inhibit gpl20-mediated CGRP release in peripheral nerves thus propose VRl as a central target for the interference with HTV-mediated neuropathies and neuropathic pain states.

Table 5: CGRP-release ([ng CGRP/mg nerve dry weight]; to-t_o: stimulation period) from rat sciatic nerve (Method C) upon stimulation with gpl20 (250 pg/ml) in the presence or absence of CAZ (1 μM) or Cpd. 1 (20 nM) (2.3.3 Method C).

Figure 5: Inhibition of gpl20-mediated CGRP release form rat sciatic nerve by Capsazepin and Cpd 1 (2.3.3. Method C).

RAB buffer was exchanged, the nerve incubated for 5 min and subsequently stimulated for 10 min with gpl20 HTV-lπi_B (250 pg/ml; black squares) or control peptide (250 pg/ml; black crosses) in the presence of Capsazepin (1 μM; white circles), compound of example 1 (20 nM; white squares) or 0.1% DMSO as control (black squares and crosses). After 5 min, 10 min and 20 min supernatant was substituted with fresh solution or RAB buffer pH 7.4 and the samples placed on ice until analysis. All samples were analyzed by EIA for CGRP release and normalized by the dry weight of the particular sciatic nerve. In summary, data presented here demonstrate for the first time that

(i) HTV envelope protein gpl20 induces stimulation of primary DRG neurons and peripheral nerves and the release of the algetic neuropeptide CGRP;

(ii) Neuronal stimulation by gpl20 directly modulates the molecular parameters of VRl ;

(iii) Gpl20-mediated CGRP release can be blocked by MEK-1 inhibitors (PD98059 and U0126), a p38 inhibitor (SB203580), a PLA₂ inhibitor (Quinacrine (QN)), a 12- and 15- LOX inhibitor (Nordihydroguaiaretic Acid (NDGA)) and in particular by VRl inhibitors (Capsazepin (CAZ) and Cpd 1). In contrast, COX inhibitors (Ibuprofen) can not inhibit gpl20-mediated CGRP release from DRG neurons;

Thus, gpl20-mediated neuronal stimulation utilizes a novel signaling cascade including, MAPKKs, MAPKs, PLA₂, and 12-LOX leading to the production of endovanilloids, such as 12- HPETE and 12-HETE. Upon binding to VRl, endovanilloids induce Ca²⁺ influx from the extracellular space, neuronal depolarization, neuronal activation and the release of neuropeptides, in particular nociceptive neuropeptides such as Substance P [Oh et al. (2001)] and CGRP (Figure 1-5). These neuropeptides are responsible for pain perception in vivo [Willis et al. (2001)]. In conclusion, gpl20-mediated neuronal stimulation is initiated upon binding of gpl20 to CXCR4 but is executed upon activation of VRl.

HTV-associated neuropathic pain, in particular DSP, is comparable to cancer pain and develops in 30% of HTV patients and almost 100% of AIDS patients [Brinley et al. (2001)]. Current therapies are widely unsatisfying due to their low efficacy [Brinley et al. (2001); Glare (2001); Verma (2001); Paice et al. (2000)]. In addition, several drugs frequently used for the treatment of other neuropathic pain states, have been proven ineffective in clinical trails [Paice et al. (2000); Shlay et al. (1998); Kieburtz et al. (1998)]. Thus, the medical need for innovative drugs specifically targeting HTV-mediated nociceptive pathways is exceedingly high. Date presented here, elucidate how HTV stimulates nocicpeptive neurons and provides several potential targets to interfere with HTV- mediated nociception. In particular, VRl antagonists prove to be highly efficient in blocking HTV- mediated release of nociceptive neuropeptides, thus reducing HTV-associated neuropathic pain. In contrast to Opoids and NSATDs, which show little efficacy and display several objectionable side effects, NRl antogonists can be expected highly efficient with few adverse effects [Walker et al. (2003); Rigoni et al. (2003); Pomonis et al. (2003)].

Neuronal cell death frequently occurs upon chronic stimulation leading to pathologically prolonged Ca²⁺ influx [Kaul et al. (2001)]. High intracellular Ca²⁺ concentrations that can not be coun- terbalanced by transport of Ca²⁺ to the ER, the mitochondria or the extracellular space induce programmed cell death [Jambrina et al. (2003); Leist and Jaattela (2001)]. It is generally accepted, that pathways leading to neuronal stimulation and neuronal cell death are identical [Kaul et al. (2001); Leist and Jaattela (2001)]. Dependent on the duration these pathways lead to stimulation or initiation of neuronal cell death [Kaul et al. (2001)]. Neuropathic pain includes chronic neuronal stimulation paired with neurotoxicity [Glare (2001)] and chronic stimulation of VRl by vanilloids and endovanilloids induces neuronal cell death in vitro and in vivo [Yamaji et al. (2003); Jambrina E et al. (2003); Herberg U and Sagen J (2001)]. Thus, it is very likely that inhibition of gpl20-induced signaling using VRl antagonists interferes not only with stimulation of nociceptive neurons but also inhibits neuronal cell death - this means that VRl antagonists may act as neuroprotective agents in HTV and AIDS patients.

Similar to the PNS, CXCR4 and VRl are co-expressed in the CNS, particularly in the brain. [Mezey et al. (2000); Kaul et al. (2001)]. Thus, the use of VRl antagonists may be extended to central HIN-associated neuropathies, such as HIN-associated dementia (HAD) and minor cognitive/motor disorder (MCMD). Highly similar to peripheral gpl20-associated neuropathies, HAD and MCMD are characterized by gpl20-mediated chronic neuronal stimulation, increased and prolonged intracellular Ca²⁺ concentrations and subsequent neuronal cell death [Kaul et al. (2001)].

In conclusion, the invention relates to the use of NRl antagonists for the treatment of HIN- associated neuropathies and neuropathic pain states in the PΝS and CΝS interfering with the nociceptive and neurotoxic properties of HTV.

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6 ABREVIATIONS

AA arachidonic acid AC Adenylyl Cyclase AIDS Acquired Immune Deficiency Syndrome

BSA Bovine serum albumin

C Cysteine

CAPS Capsaicin

CAZ Capsazepin

CCR CC Chemokine receptor

CD Cluster of differentiation

CGRP calcitonin gene-related peptide

CMV Cytomegalovirus

CNS Central nervous system

COX Cyclooxygenase

CXCR CXC chemokines receptor

DAG 1 ,2-sn-Diacylglycerol

DMSO Dimethylsulfoxide

DNA Desoxyribonucleic acid

DRG Dorsal root ganglion

DSP distal sensory painful polyneuropathy

EC Efficiency concentration

EIA Enzyme immuno assay

ERK Extracellular-signal regulated kinase et al. et alii FCA Freund's complete adjuvant

FIA Freund's incomplete adjuvans

Gp Glycoprotein

GPCR G-protein-coupled receptor

HAD HIV-associated dementia

HHV Human Herpes Virus

HTV Human Immunodeficiency Virus

HPETE hydroperoxyeicosatetraenoic acid

Hu human

HZV Herpes zoster virus

I Tnositol

IBU Ibuprofen

IC Inhibitory concentration

Ig Immunoglobulin

LOX Lipoxygenase LT Leukotriene MAPK mitogen-activated protein kinase

MCMD minor cognitive/motor disorder

MEK mitogen-activated protein kinase kinase

MIP macrophage inhibitory protein

Mu murine

MW Molecular weight

N₂ Nitrogen

N₂0 Dinitrogenoxide

NDGA Nordihydroguaiaretic acid

0₂ Oxygen

ON over night

QN Quinarine

PBS Phosphate buffered saline pH potentia hydrogeni

PI Phosphatidylinositol PKA Protein Kinase A

PKB Protein Kinase B

PKC Protein Kinase C

PLA Phospholipase A

PLC Phospholipase C

RAB Release assay buffer

RT Room temperature (20-25°C)

SDF Stroma-derived factor

SN supernatant

VR Vanilloid receptor v/v Volume per volume v/w Volume per weight

Claims

Methods of screening for VRl antagonists useful for the treatment of HTV-mediated neuropathy or HTV-mediated pain.

Methods of screening for VRl antagonists useful for the treatment of HTV-mediated neuropathy or HTV-mediated pain using Ca²⁺-sensitive dyes of bioluminescence assays in cell- free or cellular assay systems.

The use of a VRl antagonist for the preparation of a pharmaceutical composition for the treatment of HTV-mediated neuropathy or HTV-mediated pain.

The use according to claim 3, wherein HTV-mediated neuropathy or pain is caused by a disorder comprised in a group of disorders consisting of acute and chronic inflammatory demyelinating polyneuropathy, diffuse infiltrative lymphocytosis syndrome with neuropathy, mononeuropathy multiplex, progressive polyneurophathy, Cytomegalovirus (CMV)- related neuropathies, Herpes zoster virus (HZV)-related neuropathies, distal sensory painful polyneuropathy (DSP), and HTV-associated dementia.

A pharmaceutical composition containing a VRl antagonist for the treatment of HTV- mediated neuropathy or HTV-mediated pain.

The pharmaceutical composition according to claim 5, wherein HTV-mediated neuropathy or pain is caused by a disorder comprised in a group of disorders consisting of acute and chronic inflammatory demyelinating polyneuropathy, diffuse infϊltrative lymphocytosis syndrome with neuropathy, mononeuropathy multiplex, progressive polyneurophathy, Cytomegalovirus (CMN)-related neuropathies, Herpes zoster virus (HZV)-related neuropathies, distal sensory painful polyneuropathy (DSP), and HTV-associated dementia.

A pharmaceutical composition containing a VRl antagonist for the treatment of HTV- mediated neuropathy or HTV-mediated pain where the active ingredient is the compound 1 :

8. The pharmaceutical composition according to claim 7, wherein HIV-mediated neuropathy or pain is caused by a disorder comprised in a group of disorders consisting of acute and chronic inflammatory demyelinating polyneuropathy, diffuse infiltrative lymphocytosis syndrome with neuropathy, mononeuropathy multiplex, progressive polyneurophathy, Cytomegalovirus (CMN)-related neuropathies, Herpes zoster virus (HZV)-related neuropathies, distal sensory painful polyneuropathy (DSP), and HTV-associated dementia.

9. The use of a VRl antagonist for the regulation of VRl activity in a subject having HTV- mediated neuropathy or HTV-mediated pain.

10. The use according to claim 9, wherein the VRl antagonist is compound 1 :

11. The use of a 12-LOX antagonist for the preparation of a pharmaceutical composition for the treatment of HTV-mediated neuropathy or HTV-mediated pain.

12. The use according to claim 11 wherein HTV-mediated neuropathy or pain is caused by a disorder comprised in a group of disorders consisting of acute and chronic inflammatory demyelinating polyneuropathy, diffuse infiltrative lymphocytosis syndrome with neuropathy, mononeuropathy multiplex, progressive polyneurophathy, Cytomegalovirus (CMN)- related neuropathies, Herpes zoster virus (HZN)-related neuropathies, distal sensory painful polyneuropathy (DSP), and HTV-associated dementia.

13. The use of a PLA₂ antagonist for the preparation of a pharmaceutical composition for the treatment of HTV-mediated neuropathy or HTV-mediated pain.

14. The use according to claim 13 wherein HTV-mediated neuropathy or pain is caused by a disorder comprised in a group of disorders consisting of acute and chronic inflammatory demyelinating polyneuropathy, diffuse infϊltrative lymphocytosis syndrome with neuropathy, mononeuropathy multiplex, progressive polyneurophathy, Cytomegalovirus (CMV)- related neuropathies, Herpes zoster virus (HZV)-related neuropathies, distal sensory painful polyneuropathy (DSP), and HTV-associated dementia.

15. The use of a MAPK antagonist for the preparation of a pharmaceutical composition for the treatment of HTV-mediated neuropathy or HTV-mediated pain.

16. The use according to claim 15 wherein HTV-mediated neuropathy or pain is caused by a disorder comprised in a group of disorders consisting of acute and chronic inflammatory demyelinating polyneuropathy, diffuse infiltrative lymphocytosis syndrome with neuropathy, mononeuropathy multiplex, progressive polyneurophathy, Cytomegalovirus (CMV)- related neuropathies, Herpes zoster virus (HZV)-related neuropathies, distal sensory painful polyneuropathy (DSP), and HTV-associated dementia.

17. The use of a CXCR4 antagonist for the preparation of a pharmaceutical composition for the treatment of HIN-mediated neuropathy or HIN-mediated pain.

18. The use according to claim 17 wherein HIN-mediated neuropathy or pain is caused by a disorder comprised in a group of disorders consisting of acute and chronic inflammatory demyelinating polyneuropathy, diffuse infiltrative lymphocytosis syndrome with neuropathy, mononeuropathy multiplex, progressive polyneurophathy, Cytomegalovirus (CMN)- related neuropathies, Herpes zoster virus (HZV)-related neuropathies, distal sensory painful polyneuropathy (DSP), and HTV-associated dementia.

19. The use of an antagonist or modulator interfering upstream of CXCR4 ligands for the preparation of a pharmaceutical composition for the treatment of HTV-mediated neuropathy or HTV-mediated pain.

20. The use according to claim 19 wherein HTV-mediated neuropathy or pain is caused by a disorder comprised in a group of disorders consisting of acute and chronic inflammatory demyelinating polyneuropathy, diffuse infϊltrative lymphocytosis syndrome with neuropathy, mononeuropathy multiplex, progressive polyneurophathy, Cytomegalovirus (CMV)- related neuropathies, Herpes zoster virus (HZN)-related neuropathies, distal sensory painful polyneuropathy (DSP), and HIN-associated dementia.