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WO2004110964A3 - Encoded molecules by translation (emt) - Google Patents

Encoded molecules by translation (emt) Download PDF

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Publication number
WO2004110964A3
WO2004110964A3 PCT/DK2004/000416 DK2004000416W WO2004110964A3 WO 2004110964 A3 WO2004110964 A3 WO 2004110964A3 DK 2004000416 W DK2004000416 W DK 2004000416W WO 2004110964 A3 WO2004110964 A3 WO 2004110964A3
Authority
WO
WIPO (PCT)
Prior art keywords
peptides
templated molecules
synthesise
vinylogous
methods
Prior art date
Application number
PCT/DK2004/000416
Other languages
French (fr)
Other versions
WO2004110964A2 (en
Inventor
Henrik Pedersen
Thomas Franch
Original Assignee
Nuevolution As
Henrik Pedersen
Thomas Franch
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to US47834303P priority Critical
Priority to US60/478,343 priority
Priority to DKPA200300893 priority
Priority to DKPA200300893 priority
Application filed by Nuevolution As, Henrik Pedersen, Thomas Franch filed Critical Nuevolution As
Publication of WO2004110964A2 publication Critical patent/WO2004110964A2/en
Publication of WO2004110964A3 publication Critical patent/WO2004110964A3/en

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1062Isolating an individual clone by screening libraries mRNA-Display, e.g. polypeptide and encoding template are connected covalently
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1068Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67General methods for enhancing the expression

Abstract

The present invention is directed to methods for the synthesis, selection, amplification and isolation of templated molecules having desirable properties. The invention makes it possible to synthesise a variety of different templated molecules other than α-peptides and modified α-peptides. In particular, the present invention enables synthesis of templated molecules such as β -, ϒ -, ϖ peptides, carbopeptoids, vinylogous peptides, oligoanthrilamides, oligoureas, azapeptides (azatides), oligocarbamates, PNA, oligopyrrolinones, vinylogous sulfonamidopeptide, peptoids, azapeptoids and hydrazino peptides. It is possible in accordance with the methods of the invention to synthesise templated molecules comprising heterocyclic components, such as coumarins and quinolones, pyrazolone, isoxazolone, pyrimidiones, phtalhydrazides, diketopiperazines, hydantoins, and benzodiazepines.
PCT/DK2004/000416 2003-06-16 2004-06-15 Encoded molecules by translation (emt) WO2004110964A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
US47834303P true 2003-06-16 2003-06-16
US60/478,343 2003-06-16
DKPA200300893 2003-06-16
DKPA200300893 2003-06-16

Publications (2)

Publication Number Publication Date
WO2004110964A2 WO2004110964A2 (en) 2004-12-23
WO2004110964A3 true WO2004110964A3 (en) 2005-03-31

Family

ID=33553693

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK2004/000416 WO2004110964A2 (en) 2003-06-16 2004-06-15 Encoded molecules by translation (emt)

Country Status (1)

Country Link
WO (1) WO2004110964A2 (en)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8206901B2 (en) 2002-10-30 2012-06-26 Nuevolution A/S Method for the synthesis of a bifunctional complex
US8808984B2 (en) 2002-03-15 2014-08-19 Neuvolution A/S Method for synthesising templated molecules
US8932992B2 (en) 2001-06-20 2015-01-13 Nuevolution A/S Templated molecules and methods for using such molecules
US9096951B2 (en) 2003-02-21 2015-08-04 Nuevolution A/S Method for producing second-generation library
US9121110B2 (en) 2002-12-19 2015-09-01 Nuevolution A/S Quasirandom structure and function guided synthesis methods
US9574189B2 (en) 2005-12-01 2017-02-21 Nuevolution A/S Enzymatic encoding methods for efficient synthesis of large libraries

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7915201B2 (en) 2003-03-20 2011-03-29 Nuevolution A/S Ligational encoding of small molecules
DE602005017370D1 (en) 2004-03-22 2009-12-10 Nuevolution As Ligation coding using of oligonucleotide building
US7972992B2 (en) 2003-12-17 2011-07-05 Praecis Pharmaceuticals, Inc. Methods for synthesis of encoded libraries
US7972994B2 (en) 2003-12-17 2011-07-05 Glaxosmithkline Llc Methods for synthesis of encoded libraries
AT461279T (en) * 2005-06-07 2010-04-15 Harvard College Ordered multi-step synthesis by nucleic acid-mediated chemical
WO2006135654A2 (en) * 2005-06-07 2006-12-21 President And Fellows Of Harvard College Polymer evolution via templated synthesis related applications
WO2007053358A2 (en) 2005-10-28 2007-05-10 Praecis Pharmaceuticals, Inc. Methods for identifying compounds of interest using encoded libraries
WO2007142757A2 (en) 2006-04-25 2007-12-13 Massachusetts Institute Of Technology Compositions and methods for affecting movement of contaminants, bodily fluids or other entities and/or affecting other physiological conditions
WO2013033636A2 (en) 2011-09-01 2013-03-07 University Of Southern California Methods for preparing high throughput peptidomimetics, orally bioavailable drugs and compositions containing same

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19646372C1 (en) * 1995-11-11 1997-06-19 Evotec Biosystems Gmbh Conjugates of polypeptide and encoding nucleic acid
WO1998031700A1 (en) * 1997-01-21 1998-07-23 The General Hospital Corporation Selection of proteins using rna-protein fusions
EP0962527A1 (en) * 1996-10-17 1999-12-08 Mitsubishi Chemical Corporation Molecule that homologizes genotype and phenotype and utilization thereof
WO2000023458A1 (en) * 1998-10-19 2000-04-27 The Board Of Trustees Of The Leland Stanford Junior University Dna-templated combinatorial library chemistry
WO2001007657A1 (en) * 1999-07-27 2001-02-01 Phylos, Inc. Peptide acceptor ligation methods
WO2002059293A2 (en) * 2001-01-25 2002-08-01 Forster Anthony C Process and compositions for peptide, protein and peptidomimetic synthesis
WO2002074929A2 (en) * 2001-03-19 2002-09-26 President And Fellows Of Harvard College Evolving new molecular function
WO2002085923A2 (en) * 2001-04-19 2002-10-31 The Scripps Research Institute In vivo incorporation of unnatural amino acids
WO2002103008A2 (en) * 2001-06-20 2002-12-27 Nuevolution A/S Templated molecules and methods for using such molecules

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19646372C1 (en) * 1995-11-11 1997-06-19 Evotec Biosystems Gmbh Conjugates of polypeptide and encoding nucleic acid
EP0962527A1 (en) * 1996-10-17 1999-12-08 Mitsubishi Chemical Corporation Molecule that homologizes genotype and phenotype and utilization thereof
WO1998031700A1 (en) * 1997-01-21 1998-07-23 The General Hospital Corporation Selection of proteins using rna-protein fusions
WO2000023458A1 (en) * 1998-10-19 2000-04-27 The Board Of Trustees Of The Leland Stanford Junior University Dna-templated combinatorial library chemistry
WO2001007657A1 (en) * 1999-07-27 2001-02-01 Phylos, Inc. Peptide acceptor ligation methods
WO2002059293A2 (en) * 2001-01-25 2002-08-01 Forster Anthony C Process and compositions for peptide, protein and peptidomimetic synthesis
WO2002074929A2 (en) * 2001-03-19 2002-09-26 President And Fellows Of Harvard College Evolving new molecular function
WO2002085923A2 (en) * 2001-04-19 2002-10-31 The Scripps Research Institute In vivo incorporation of unnatural amino acids
WO2002103008A2 (en) * 2001-06-20 2002-12-27 Nuevolution A/S Templated molecules and methods for using such molecules

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
FORSTER ANTHONY C ET AL: "Programming peptidomimetic syntheses by translating genetic codes designed de novo.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 100, no. 11, 27 May 2003 (2003-05-27), pages 6353 - 6357, XP002316170, ISSN: 0027-8424 *

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8932992B2 (en) 2001-06-20 2015-01-13 Nuevolution A/S Templated molecules and methods for using such molecules
US8808984B2 (en) 2002-03-15 2014-08-19 Neuvolution A/S Method for synthesising templated molecules
US8206901B2 (en) 2002-10-30 2012-06-26 Nuevolution A/S Method for the synthesis of a bifunctional complex
US8722583B2 (en) 2002-10-30 2014-05-13 Nuevolution A/S Method for selecting a chemical entity from a tagged library
US9109248B2 (en) 2002-10-30 2015-08-18 Nuevolution A/S Method for the synthesis of a bifunctional complex
US9284600B2 (en) 2002-10-30 2016-03-15 Neuvolution A/S Method for the synthesis of a bifunctional complex
US9121110B2 (en) 2002-12-19 2015-09-01 Nuevolution A/S Quasirandom structure and function guided synthesis methods
US9096951B2 (en) 2003-02-21 2015-08-04 Nuevolution A/S Method for producing second-generation library
US9574189B2 (en) 2005-12-01 2017-02-21 Nuevolution A/S Enzymatic encoding methods for efficient synthesis of large libraries

Also Published As

Publication number Publication date
WO2004110964A2 (en) 2004-12-23

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