WO2004104221A3 - Methods and kits for quantifying metabolites or analytes - Google Patents

Methods and kits for quantifying metabolites or analytes Download PDF

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Publication number
WO2004104221A3
WO2004104221A3 PCT/GB2004/002227 GB2004002227W WO2004104221A3 WO 2004104221 A3 WO2004104221 A3 WO 2004104221A3 GB 2004002227 W GB2004002227 W GB 2004002227W WO 2004104221 A3 WO2004104221 A3 WO 2004104221A3
Authority
WO
WIPO (PCT)
Prior art keywords
metabolite
enzyme
analyte
sample
reaction product
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB2004/002227
Other languages
French (fr)
Other versions
WO2004104221A2 (en
Inventor
Graham Herbert Coombs
Jeremy Charles Mottram
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Glasgow
Original Assignee
University of Glasgow
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Glasgow filed Critical University of Glasgow
Priority to EP04734293A priority Critical patent/EP1625227A2/en
Priority to US10/558,240 priority patent/US20070065896A1/en
Priority to AU2004241364A priority patent/AU2004241364A1/en
Priority to CA002531337A priority patent/CA2531337A1/en
Priority to JP2006530533A priority patent/JP2007501019A/en
Publication of WO2004104221A2 publication Critical patent/WO2004104221A2/en
Publication of WO2004104221A3 publication Critical patent/WO2004104221A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6806—Determination of free amino acids
    • G01N33/6812—Assays for specific amino acids
    • G01N33/6815—Assays for specific amino acids containing sulfur, e.g. cysteine, cystine, methionine, homocysteine

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Methods and kits for quantifying the amount of a metabolite, such as homocysteine, methionine or cysteine, in a sample are disclosed. This involves contacting the sample with a first enzyme capable of degrading a metabolite into a plurality of reaction products and regenerating the metabolite using a second enzyme which is capable of converting at least one of said reaction product(s), together with an additional substrate or substrates, into said metabolite. This process optionally generates an additional by-product or by-products. After repeating this process, the metabolite is quantified by detecting a level of a reaction product formed by enzyme degradation of said metabolite by said first enzyme, wherein the reaction product is different from the reaction product used in regenerating said metabolite, or detecting a level of said optional by-product produced by said second enzyme or detecting a level of an additional substrate. The method may also involve the initial conversion of an analyte in a sample to a metabolite by contacting the analyte with a reducing agent. The present method further provides a method of quantifying the amount of an analyte in sample by reacting the analyte to produce a metabolite in a cyclic reaction.
PCT/GB2004/002227 2003-05-22 2004-05-21 Methods and kits for quantifying metabolites or analytes Ceased WO2004104221A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
EP04734293A EP1625227A2 (en) 2003-05-22 2004-05-21 Methods and kits for quantifying metabolites or analytes
US10/558,240 US20070065896A1 (en) 2003-05-22 2004-05-21 Methods and kits for quantifying metabolites or analytes
AU2004241364A AU2004241364A1 (en) 2003-05-22 2004-05-21 Methods and kits for quantifying metabolites or analytes
CA002531337A CA2531337A1 (en) 2003-05-22 2004-05-21 Methods and kits for quantifying metabolites or analytes
JP2006530533A JP2007501019A (en) 2003-05-22 2004-05-21 Methods and kits for quantifying metabolites or analytes

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0311804.9 2003-05-22
GBGB0311804.9A GB0311804D0 (en) 2003-05-22 2003-05-22 Improved homocysteine assay

Publications (2)

Publication Number Publication Date
WO2004104221A2 WO2004104221A2 (en) 2004-12-02
WO2004104221A3 true WO2004104221A3 (en) 2005-03-17

Family

ID=9958592

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2004/002227 Ceased WO2004104221A2 (en) 2003-05-22 2004-05-21 Methods and kits for quantifying metabolites or analytes

Country Status (7)

Country Link
US (1) US20070065896A1 (en)
EP (1) EP1625227A2 (en)
JP (1) JP2007501019A (en)
AU (1) AU2004241364A1 (en)
CA (1) CA2531337A1 (en)
GB (1) GB0311804D0 (en)
WO (1) WO2004104221A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0518460D0 (en) * 2005-09-09 2005-10-19 Univ Nottingham Trent Protein assay
JP5303715B2 (en) * 2012-02-09 2013-10-02 富山県 Amino acid quantification method using pyrophosphate quantification
CN104136627A (en) 2012-02-09 2014-11-05 富山县政府 Method for quantifying target substance
CN104849396B (en) * 2015-05-28 2016-06-29 浙江普康生物技术股份有限公司 A kind of detection method measuring L900 cleaning mixture residual quantity
EP3918087A1 (en) * 2019-01-31 2021-12-08 Roche Diagnostics GmbH Stabilization of nadph or nadh in ammonia detection assays
CN113957116B (en) * 2021-11-01 2023-05-30 温州医科大学 Method for detecting L-serine based on living cells of escherichia coli containing cysteine desulphurase
CN115960878B (en) * 2023-01-18 2025-07-11 浙江工业大学 O-succinylthiolyase mutant, encoding gene and application

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5885767A (en) * 1998-05-22 1999-03-23 Biocatalytics, Inc. Methods and compositions for quantitating L-homocysteine and/or l-methionine in a solution
US6306618B1 (en) * 1996-08-23 2001-10-23 University Of The University Of Glasgow Homocysteine desulphurase from the protozoan trichomonas vaginalis
WO2003040694A2 (en) * 2001-11-06 2003-05-15 Catch, Inc. Enzymatic cycling assays for homocysteine and cystathionine

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6306618B1 (en) * 1996-08-23 2001-10-23 University Of The University Of Glasgow Homocysteine desulphurase from the protozoan trichomonas vaginalis
US5885767A (en) * 1998-05-22 1999-03-23 Biocatalytics, Inc. Methods and compositions for quantitating L-homocysteine and/or l-methionine in a solution
WO2003040694A2 (en) * 2001-11-06 2003-05-15 Catch, Inc. Enzymatic cycling assays for homocysteine and cystathionine

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
SHIMIZU^A H ET AL: "Cloning and overexpression of the oah1 gene encoding O-acetyl-l-homoserine sulfhydrylase of Thermus thermophilus HB8 and characterization of the gene product", BIOCHIMICA ET BIOPHYSICA ACTA. PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, ELSEVIER, AMSTERDAM,, NL, vol. 1549, no. 1, 10 September 2001 (2001-09-10), pages 61 - 72, XP004305697, ISSN: 0167-4838 *
YAMAGATA S: "ROLES OF O-ACETYL-L-HOMOSERINE SULFHYDRYLASES IN MICRO-ORGANISMS", BIOCHIMIE, MASSON, PARIS, FR, vol. 71, no. 11/12, 1989, pages 1125 - 1143, XP001037839, ISSN: 0300-9084 *

Also Published As

Publication number Publication date
US20070065896A1 (en) 2007-03-22
CA2531337A1 (en) 2004-12-02
JP2007501019A (en) 2007-01-25
GB0311804D0 (en) 2003-06-25
EP1625227A2 (en) 2006-02-15
WO2004104221A2 (en) 2004-12-02
AU2004241364A1 (en) 2004-12-02

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