WO2004090154A2

WO2004090154A2 - Acyl- phosphate and phosphonate probes and methods of their synthesis and use in proteomic analysis

Info

Publication number: WO2004090154A2
Application number: PCT/US2004/010075
Authority: WO
Inventors: David Alan Campbell; Marek Liyanage; Anna Katrin Szardenings; Min Wu
Original assignee: Activx Biosciences, Inc.
Priority date: 2003-04-01
Filing date: 2004-04-01
Publication date: 2004-10-21
Also published as: US20050043507A1; EP1616034A2; CA2521130C; CA2521130A1; JP2006526010A; JP4742029B2; AU2004227362B2; WO2004090154A3; AU2004227362A1; US7365178B2; EP1616034A4

Abstract

The present invention provides tagged acyl phosphate probes ('TAPPs'), and methods of their preparation and use. The subject methods and compositions can provide enhanced simplicity and accuracy in identifying changes in the presence, amount, or activity of target proteins in a complex protein mixture, preferably nucleotide binding proteins using nucleotide binding protein-directed TAPPs. The profiling methods described herein can have a number of steps leading to the identification of target nucleotide binding protein(s) in a complex protein mixture.

Description

ACYX-PHOSPHATE AND PHOSPHONATE PROBES AND METHODS OF THEIR

SYNTHESIS AND USE IN PROTEOMIC ANALYSIS

RELATED APPLICATION

[0001] This application claims the benefit of U.S. Provisional application

60/459,797, filed April 1, 2003, which is incorporated herein by reference in its entirety, including drawings.

FIELD OF THE INVENTION [0002] The invention relates generally to compositions and methods for labeling proteins, especially nucleotide binding proteins, preferably kinases, and most preferably protein kinases, using tagged acyl phosphate derivatives.

BACKGROUND [0003] Nucleotide-binding proteins play an extremely important role as regulators of genomic and proteomic function. Examples of nucleotide binding proteins include G proteins, which act as coupling factors in association with certain receptors; protein kinases, which transfer a phosphate group to target proteins; non-protein kinases, such as hexokinase, which are involved in the metabolic pathways within cells; proteins utilizing the energy stored within nucleotide-based molecules such as ATP; etc. [0004] Protein kmases are the enzymes responsible for catalyzing the transfer of a γ- phosphoryl group from ATP to the hydroxyl group of serine, threonine or tyrosine residues in peptides, polypeptides, and proteins in a process known as "phosphorylation." Protein phosphorylation is a ubiquitous regulatory mechanism in eukaryotic cells, where it is of central importance in controlling cell function, growth and differentiation. A protein kinase that phosphorylates, for example, tyrosine residues in its substrates is termed a protein- tyrosine:ATP phosphotransferase, or, more commonly, a tyrosine (or Tyr) kinase. The eukaryotic protein kinases make up a large superfamily of related proteins. They are related by virtue of their kinase domains (also known as catalytic domains), which consist of approximately 250-300 amino acid residues. The kinase domains that define this group of enzymes contain 12 conserved subdomains that fold into a common catalytic core structure. See, e.g., Hanks and Hunter, FASEB J. (1995) 9(8):576-96.

[0005] Eukaryotic protein kinases can be classified on the basis of their sequence, substrate specificity and regulation. One major subdivision is between Ser/Thr kinases and the Tyr kinases. Yeast have numerous Ser/Thr kinases, many of which have readily recognizable counterparts in all higher organisms, but very few dedicated Tyr kinases (an example of a yeast Tyr kinase is Swel from Saccharomyces cerevisiae and its homolog in S. pombe Weel). By contrast, many signaling pathways of multicellular organisms depend on two large and important Tyr kinase families, the receptor-Tyr kinases which have intracellular Tyr kinase domains, and the Src family of cytoplasmic Tyr kinases. There are also dual-specificity enzymes, present in both unicellular and multicellular eukaryotes, such as the mitogen-activated protein kinase kinases (MAPKKs).

[0006] Overexpression and/or mutation of certain kinases in tumor cell is believed to upregulate a number of cell cycle and anti-apoptosis pathways leading to subversion of cell cycle checkpoints and enhanced cancer cell survival and metastatic potential. Conversely, inhibition of these kinases may reverse the aberrant signaling in receptor- overexpressing cells and may result in growth arrest and/or tumor cell death. Thus, it is no surprise that kinases have been considered important targets for the identification of therapeutics. See, e.g., Bishop et al., Trends Cell Biol (2001) 11(4): 167-72.

SUMMARY OF THE INVENTION [0007] The present invention provides compositions and methods for assessing protein profiles in biological samples. In various embodiments, one or more samples, most preferably one or more complex protein mixtures as defined below, are contacted with one or more probes, referred to herein as "tagged acyl phosphate probes" or "TAPPs." These probes, have the following general structure:

O O

TAG- -O- -X

O-

wherein TAG is a detectable label, L is a linker moiety covalently bound to the carbonyl through a carbon atom, and X is an affinity moiety for directing the binding of a TAPP to a set of target proteins. In preferred embodiments, X is linked through a carbon to form an acyl phosphonate, but is most preferably linked through an oxygen to form an acyl phosphate. The skilled artisan will understand that the activated acyl group of such a structure readily forms protein-bound adducts by reaction with nucleophilic groups such as an amino group on target protein molecules.

[0008] TAPPs are described herein in terms of nucleotide binding protein-directed affinity probes" or "NBAPs," comprising: a nucleotide or nucleotide analogue covalently bound tlirough the terminal phosphate of a 5' mono- di- or tri-phosphate to an acyl group, which is itself further covalently bound to a detectable tag via a linker moiety. As described hereinafter, the nucleotide portion directs the binding of an NB AP to nucleotide binding proteins, or proteins intimately associated with nucleotide binding proteins. But the skilled artisan will understand that the affinity moiety X of a TAPP may be varied widely to provide probes directed to a number of proteins or protein families. [0009] The binding selectivity of the probe(s) may be selected to allow the skilled artisan to analyze the presence, amount, and/or activity of a selected portion of the nucleotide binding proteins present in a sample, thereby simplifying the analysis of complex protein mixtures.

[0010] One or more TAPPs are combined with a protein-containing sample under conditions for binding and reaction of the TAPP(s) with target proteins that are present in the sample. The resulting products are then used to assess the target protein profile of the sample, and can be correlated to the presence, amount, or activity of one or more target proteins present in the original complex protein mixture.

[0011] In a first aspect, the present invention relates to methods and compositions for determining an enzyme profile in a complex protein mixture. These methods comprise contacting the complex protein mixture with one or more distinct TAPPs, each of which specifically reacts with one or more target proteins, preferably target nucleotide binding proteins, and most preferably target kinases. The labeled protein profile can then be analyzed by the screening and/or identification methods described hereinafter. [0012] In preferred embodiments, the TAPP-protein conjugates can be separated from other components of the complex protein mixture, for example by sequestering one or more conjugates (e.g., by binding to a receptor that binds the TAG portion of the TAPP or by using a "tethered" TAPP), by chromatographic methods, by mass spectrographic methods, and/or by other means such as electrophoresis. Thus, in related aspects, the present invention also relates to purified polypeptides (e.g., proteins or protein fragments) bound to TAPP(s). In these aspects, the labeled polypeptides have the following structure:

O

TAG L C Polypeptide

wherein the polypeptide is covalently bound to the carbonyl through an amide, ester, or thioester linkage. [0013] In various embodiments, following reaction of the complex protein mixture with one or more TAPPs, the resulting TAPP-protein conjugates may be proteolytically digested to provide TAPP-labeled peptides. This digestion may occur while the protein conjugates are sequestered to a solid phase, or while free in solution. In preferred embodiments, TAPPs are selected such that each target protein forms a conjugate with a single TAPP, most preferably at a single discrete location in the target nucleotide binding protein; thus, each conjugate gives rise to a single TAPP-labeled peptide. Enrichment separation, or identification of one or more TAPP-labeled peptides may be achieved using liquid chromatography and/or electrophoresis. Additionally, mass spectrometry may be employed to identify one or more TAPP-labeled peptides by molecular weight and/or amino acid sequence, hi particularly preferred embodiments, the sequence information derived from the TAPP-labeled peptide(s) is used to identify the protein from which the peptide originally derived. Variations of these aspects can involve the comparison of two or more proteomes, e.g., with TAPPs having different TAGs, or, when analysis comprises mass spectrometry, having different isotopic compositions.

[0014] In yet another aspect, the instant invention relates to methods for comparing the presence, amount, or activity of one or more target proteins in two or more complex protein mixtures using the methods and compositions described herein. In various embodiments, these methods comprise one or more of the following steps: contacting one or more complex protein mixture(s) with one or more TAPPs, where the TAPP(s) specifically bind to one or more target proteins present in each complex protein mixture; combining the complex protein mixtures following this contacting step to form a combined complex protein mixture; prior to and/or following this combination, removing one or more non- sequestered components of the complex protein mixture(s). The labeled protein profile can then be analyzed by the screening and/or identification methods described hereinafter. [0015] In preferred embodiments, the methods and compositions described herein are applied to determining the nucleotide binding protein profiles of cancerous and other diseased tissue by obtaining one or more samples of diseased tissue, and determining the nucleotide binding protein profile of the tissue sample(s). In particularly preferred embodiments, the nucleotide binding protein profile of diseased tissues can be compared to that of normal tissue sample(s) to determine differences in the enzyme activity profiles of the two tissue samples.

[0016] In still another aspect, the present invention relates to methods and compositions for detecting disease in a test sample. In preferred embodiments the test sample will be a cell or tissue sample. In particularly preferred embodiments, the tissue sample will be a neoplasmic sample and the disease is a cancer. The methods involve determining the target protein profile of the test sample using one or more TAPPs; comparing the labeled protein profiles of the test sample with the labeled protein profile(s) of one or more known non-diseased sample and/or with the labeled protein profile(s) of one or more known diseased samples; and determining whether the test sample is in a state of disease. A "non-diseased" sample is a sample of cells or tissues that is known to not have the disease being tested for. It is preferably a normal, healthy sample of the cells or tissue. [0017] hi another aspect the present invention provides methods of deteπnining the inhibitory potency of a test compound against one or more target proteins. The methods involve contacting one or more TAPPs with a test sample containing the test compound and the target protein(s); allowing the TAPPs to react with proteins contained in the test sample; and detecting a signal that indicates the level of TAPP binding to the target protein(s) in the test sample.

[0018] In preferred embodiments, this level of TAPP binding is compared to the level of TAPP binding to the target protein(s) in the absence of the test compound. By such methods, the inhibitory and/or stimulatory potency of the test compound against the target protein(s) can be determined. The "inhibitory potency" is the extent to which the presence of the compound causes the inhibition of TAPP binding, while "stimulatory potency" is the extent to which the presence of the compound causes an increase in TAPP binding. [0019] Iii yet another aspect, the present invention provides kits for performing the methods described. The kits contain one or more of the materials described for conducting the methods. The kits can include TAPPs in the solid phase or in a liquid phase (such as buffers provided) in a package. The kits also can include buffers for preparing solutions for conducting the methods, and pipettes for transferring liquids from one container to another. By "package" is meant material enveloping a vessel containing the TAPPs. In preferred embodiments, the package can be a box or wrapping. The kit can also contain items that are not contained within the package but are attached to the outside of the package, for example, pipettes.

[0020] The summary of the invention described above is not limiting and other features and advantages of the invention will be apparent from the following detailed description of the preferred embodiments, as well as from the claims.

BRIEF DESCRIPTION OF THE FIGURES [0021] Fig. 1 shows exemplary acyl phosphate probes of the invention.

[0022] Fig. 2 shows an exemplary synthesis scheme for preparing acyl phosphate probes of the invention.

[0023] Fig. 3 shows an alternative exemplary synthesis scheme for preparing acyl phosphate probes of the invention.

[0024] Fig. 4 shows exemplary BASEs for use in preparing acyl phosphate probes of the invention. [0025] Fig. 5 shows exemplary affinity moieties for use in preparing acyl phosphate probes of the invention.

DETAILED DESCRIPTION OF THE INVENTION

[0026] The subject methods and compositions provide enhanced simplicity and accuracy in identifying changes in the presence, amount, or activity of proteins in a complex protein mixture using TAPPs. As described hereinafter, preferred TAPPs are NBAPs that bind to target nucleotide binding protein(s) and proteins that interact with nucleotide binding protein(s). The profiling methods described herein can have a number of steps leading to the identification of, or determining the presence or amount of, target protein(s) in a complex protein mixture. A complex protein mixture, and preferably two or more complex protein mixtures, e.g., a sample and a control, can be used as obtained from a natural source or as processed, e.g., to remove interfering components and/or enrich the target protein components. Each complex protein mixture to be analyzed is combined under reaction conditions with at least one TAPP to produce conjugates with target nucleotide binding protein(s). The TAPPs used in two or more complex protein mixtures can differ as to the choice of TAG moiety, linker moieties, affinity moieties, and/or isotopic composition. In preferred embodiments, the labeled complex protein mixtures may be directly compared (e.g., in the same capillary of a capillary electrophoresis apparatus or lane in an electrophoresis gel, or in a mass spectrometer).

[0027] The analysis platforms described herein can differ as to the methods of enrichment and analysis using liquid chromatography and/or electrophoresis, and/or mass spectrometry for identification and quantitation. The choice of the platform is affected by the size of the sample, the rate of throughput of the samples, the mode of identification, and the need for and level of quantitation. [0028] Of particular interest as target proteins in the present invention are nucleotide binding proteins, and most preferably protein kinases. The term "nucleotide binding protein" refers to proteins that bind nucleotide mono-, di- and/or tri-phosphates. Exemplary nucleotide binding protein families include kinase families described below; guanine nucleotide binding proteins (e.g. in G protein-coupled receptors); motor-related proteins (e.g., myosin, actin, tubulin, dynein, kinesin, etc.); nucleic acid polymerases; UspA and related proteins; P2 receptors; etc. This list is not meant to be limiting. [0029] Protein kinases are the enzymes responsible for catalyzing the transfer of a γ- phosphoryl group from ATP to the hydroxyl group of serine, threonine or tyrosine residues in peptides, polypeptides, and proteins in a process known as "phosphorylation." Protein kinases have been identified in both prokaryotes and eukaryotes, and in both plants and animals. The list of identified kinases is extensive, including the following families of proteins: cyclic nucleotide regulated protein kinase (PKA & PKG) family; diacylglycerol- activated/phospholipid-dependent protein kinase C (PKC) family; kinases that phoshorylate G protein-coupled receptors family; budding yeast AGC-related protein kinase family; kinases that phosphorylate ribosomal protein S6 family; budding yeast DBF2/20 family; flowering plant PVPKl protein kinase homolog family; kinases regulated by Ca²⁺/CaM and close relatives family; KINl/SNFl/Niml family; cyclin-dependent kinases (CDKs) and close relatives family; ERK (MAP) kinase family; glycogen synthase kinase 3 (GSK3) ^•family; casein kinase II family; Clk family; Src family; Tec/Atk family; Csk family; Fes (Fps) family; Abl family; Syk/ZAP70 family; Tyk2/Jakl family; Ack family; focal adhesion kinase (Fak) family; epidermal growth factor receptor family; Eph/Elk/Eck receptor family; Axl family; Tie/Tek family; platelet-derived growth factor receptor family; fibroblast growth factor receptor family; insulin receptor family; LTK/ALK family; Ros/Sevenless family; Trk/Ror family; DDR/TKT family; hepatocyte growth factor receptor family, nematode Kinl5/16 family; Polo family; MEK/STE7 family; PAK/STE20 family; MEKK/STE11 family; NimA family; weel/mikl family; kinases involved in transcriptional control family; Raf family; activin/TGFb receptor family; flowering plant putative receptor kinases and close relatives family; PSK/PTK "mixed lineage" leucine zipper domain family; casein kinase I family; and PKN prokaryotic protein kinase family. [0030] The compositions and methods described herein find use for the most part with biological samples, which may have been subject to processing before reaction with the TAPPs. "Biological sample" intends a sample obtained from a cell, tissue, or organism. Examples of biological samples include proteins obtained from cells (e.g., mammalian cells, bacterial cells, cultured cells, human cells, plant cells, etc.), particularly as a lysate, a biological fluid, such as blood, plasma, serum, urine, bile, saliva, tears, cerebrospinal fluid, aqueous or vitreous humor, or any bodily secretion), a transudate or exudate (e.g. fluid obtained from an abscess or other site of infection or inflammation), a fluid obtained from a joint (e.g. synovial fluid obtained from a normal joint or a joint affected by disease such as rheumatoid arthritis, osteoarthritis, gout or septic arthritis), or the like. [0031] Biological samples may be obtained from any organ or tissue (including a biopsy or autopsy specimen) or may comprise cells (including primary cells, passaged or cultured primary cells, cell lines, cells conditioned by a specific medium) or medium conditioned by cells. In preferred embodiments, a biological sample is free of intact cells. If desired, the biological sample may be subjected to prior processing, such as lysis, extraction, subcellular fractionation, and the like. See, Deutscher (ed.), 1990, Methods in Enzymology, vol. 182, pp. 147-238.

[0032] Of particular interest are samples that are "complex protein mixtures." As used herein, this phrase refers to protein mixtures having at least about 20, more usually at least about 50, even 100 or more different proteins, where the particular distribution of proteins is of interest. An example of such a complex protein mixture is a proteome, as defined hereinafter. Complex protein mixtures may be obtained from cells that are normal or abnormal in some particular, where the abnormality is informative as to treatment, status, disease, or the like, can be analyzed using the methods of the subject invention. [0033] The teπn "proteome" as used herein refers to a complex protein mixture obtained from a biological sample. Preferred proteomes comprise at least about 5% of the total repertoire of proteins present in a biological sample (e.g., the cells, tissue, organ, or organism from which a lysate is obtained; the serum or plasma, etc), preferably at least about 10%, more preferably at least about 25%, even more preferably about 75%, and generally 90% or more, up to and including the entire repertoire of proteins obtainable from the biological sample. Thus the proteome may be obtained from an intact cell, a lysate, a microsomal fraction, an organelle, a partially extracted lysate, biological fluid, a tissue, an organ, and the like. The proteome will be a mixture of proteins, generally having at least about 20 different proteins, usually at least about 50 different proteins and in most cases 100 different proteins or more.

[0034] Generally, the sample will have at least about 1 x 10^"11 g of protein, and may have 1 g of protein or more, preferably at a concentration in the range of about 0.1 - 50 mg/ml. For screening applications, the sample will typically be between about 1 x 10^"11 g of protein and about 1 x 10^"3 g of protein, preferably between about 1 x 10^"6 g of protein and 1 x 10^"4 g of protein. For identification of labeled active target kinases, the sample will typically be between about 1 x 10^"9 g of protein and about 1 g of protein, preferably between about 1 x 10^"4 g of protein and 1 x 10^"1 g of protein. The term "about" in this context refers to +/- 10%) of the amount listed.

[0035] The sample may be adjusted to the appropriate buffer concentration and pH, if desired. One or more TAPPs may then be added, each at a concentration in the range of about 1 nM to 20 mM, preferably 10 nM to 1 mM, most preferably 10 nm to 100 μM. After

incubating the reaction mixture, generally for a time for the reaction to go substantially to completion, generally for about 0.11 - 60 minutes, at a temperature in the range of about 5 - 40°C, preferably about 10°C to about 30°C , most preferably about 20°C , the reaction may be quenched.

[0036] In one aspect of the invention, the methods and compositions provide for qualitative (e.g., relative comparison between two samples) and/or quantitative measurement of target nucleotide binding protein(s)in biological fluids, cells or tissues. Moreover, the same general strategy can be broadened to achieve the proteome-wide, qualitative and quantitative analysis of target protein(s), by employing TAPPs with differing target specificities. The methods and compositions of this invention can be used to identify labeled target protein(s) of low abundance that are present in complex protein mixtures and can be used to selectively analyze specific groups or classes of proteins, such as membrane or cell surface kinases, or kinases contained within organelles, sub-cellular fractions, or biochemical fractions such as immunoprecipitates. Further, these methods can be applied to analyze differences in expressed target proteins in different cell states. For example, the methods and reagents herein can be employed in diagnostic assays for the detection of the presence or the absence of one or more target proteins indicative of a disease state, such as cancer.

[0037] The subject methods and compositions can be used for a variety of purposes, such as the diagnosis of disease, the response of cells to an external agent, e.g. a drug, staging diseases, such as neoplasia, identifying cell differentiation and maturation, identifying new proteins, screening for active drugs, determining side effects of drugs, determining selectivity of drugs, identifying responses to drugs specific to certain genotypes (e.g., allelic differences in individuals), identifying useful probes from combinatorial libraries, etc.

[0038] The system uses TAPPs that are typically directed to an active site on target protein(s). However, many proteins may be labeled, not as a result of their own interaction with a TAPP, but by their proximity to a second protein that does interact with a TAPP. For example, numerous nucleotide binding proteins (e.g., kinases, G-protein coupled receptors, etc.) are members of multisubunit complexes. An NBAP may be selected for its ability to interact with the nucleotide binding site of a particular kinase; but may bind to one or more member(s) of the complex that lie sufficiently close to that nucleotide binding site, even though the other member(s) do not themselves bind to the NBAP.

[0039] This ability to bind members of the complex may also be related to various physiological states, as it may be that the other member(s) of the complex are only sufficiently close to that nucleotide binding site under certain circumstances (e.g., when the kinase is phosphorylated, or when a cofactor is present). Similarly, different sites on a target protein may be differentially labeled in different physiological states, as when the target protein changes three-dimensional conformation under similar circumstances. [0040] In certain embodiments, a plurality of TAPPs may be combined for use in a labeling method, depending on the specificity of the TAPPs and the variety in the group or groups of proteins to be assayed. In the present invention, it is not necessary that there be no reaction of a TAPP with non-target protein(s). Rather, a TAPP is defined as being "specific for," as "specifically reacting with," or as "specifically binding to," target protein(s) if the TAPP provides at least about twice the amount of signal from TAPP labeling of target protein(s) when compared to an equivalent amount of non-target protein. Preferably the signal obtained from target protein(s) will be at least about five fold, preferably 10 fold, more preferably 25-fold, even more preferably 50-fold, and most preferably 100-fold or more, greater than that obtained from an equivalent amount of non-target protein. [0041] The term "target protein" as used herein refers to one or more protein(s), a residue of which specifically reacts with, and becomes covalently labeled by, one or more TAPPs. Preferred targets are kinases generally classified under the Enzyme Commission number 2.7.1.X. Particularly preferred kinases are protein kinases, classified under the Enzyme Commission number 2.7.1.37. The reaction mixture can provide conditions under which the TAPP(s) react substantially preferentially with functional target proteins, preferably functional target kinases. Particularly preferred target kinases include phosphorylase b kinase; glycogen synthase a kinase; hydroxyalkyl-protein kinase; serine(threonine) protein kinase; A-kinase; AP50 kinase; ATP-protein transphosphorylase;

βUPKC; β-andrenergic receptor kinase; calcium/phospholipid-dependent protein kinase;

calcium-dependent protein kinase C; cAMP-dependent protein kinase A; cAMP-dependent protein kinase; casein kinase; casein kinase I; casein kinase II; casein kinase 2; cGMP- dependent protein kinase; CK-2; CKI; CKII; cyclic monophosphate-dependent protein kinase; cyclic AMP-dependent protein kinase; cyclic AMP-dependent protein kinase A; cyclic nucleotide-dependent protein kinase; cyclin-dependent kinase; cytidine 3',5'-cyclic

monophosphate-responsive protein kinase; ε PKC; glycogen synthase kinase; Hpr kinase;

hydroxyalkyl-protein kinase; protein kinase (phosphorylating); casein kinase (phosphorylating); MAPK; mitogen-activated protein kinase; mitogen-activated S6 kinase; M phase-specific cdc2 kinase; p82 kinase; phosphorylase b kinase; PKA; PKC; protein serine kinase; protein kinase A; protein kinase p58; protein phosphokinase; protein glutamyl kinase; protein serine-threonine kinase; protein kinase CK2; protein-aspartyl kinase; protein-cysteine kinase; protein-serine kinase; Raf kinase; Raf-1; ribosomal S6 protein kinase; ribosomal protein S6 kinase II; serine kinase; serine-specific protein kinase; serine protein kinase; serine/threonine protein kinase; T-antigen kinase; threonine-specific protein kinase; twitchin kinase; and type-2 casein kinase.

[0042] The term "functional target protein" refers to a target protein that is in its native conformation and is able to interact with an entity with which it normally interacts, e.g. enzyme with substrate and/or cofactor, receptor with ligand, etc., e.g. phosphorylated active form as compared to unphosphorylated inactive form and vz^'ce versa. Preferably, the functional target protein is in the form in which it can carry out its biological function. [0043] The term "inactivated" as used herein refers to a sample that has been treated so that at least a portion of target protein(s) that were functional in the original sample are rendered unable to interact with those entities with which it normally interacts. For example, an "inactive nucleotide binding protein" can result from various mechanisms such as denaturation, inhibitor binding, either covalently or non-covalently, mutation, secondary processing, e.g. phosphorylation or dephosphorylation, etc.

[0044] The term "untreated" as used herein refers to a sample that has not been exposed to one or more conditions as compared to a second sample not exposed to such conditions. An untreated sample may be a sample that has not been inactivated; alternatively, an untreated sample may be one not exposed to one or more molecules (e.g., drug lead compounds) in a screening assay. Thus the compositions and methods described herein may comprise comparing a complex protein mixture obtained from cell(s), tissue(s), or organism(s) treated with one or more compounds (e.g., lead compounds in drug discovery) to a complex protein mixture obtained from cell(s), tissue(s), or organism(s) not so treated. TAPP-labeled proteins and/or peptides from the two samples may be compared for relative signal intensity. Such methods may indicate alterations in active protein content due to the treatment regimen. Additionally, such methods can also differentiate between treatments that act by direct inhibition of specific proteins ("primary effects") versus treatments that affect active protein content upstream, e.g., by altering expression of protein(s) ("secondary effects").

[0045] As used herein, the term "purified" in reference to labeled target proteins or polypeptides does not require absolute purity. Instead, it represents an indication that the labeled target proteins or polypeptides are relatively more pure than in the environment in which the proteins or polypeptides were labeled. A "purified" labeled target protein or polypeptide is preferably at least 10% pure. A "substantially purified" labeled target protein or polypeptide is preferably at least 50% pure, more preferably at least 75% pure, and most preferably at least 95% pure.

[0046] An "active site" of a protein refers to an area on the surface of a protein, e.g., an enzyme molecule or surface membrane receptor, to which a binding molecule, e.g. substrate, reciprocal ligand, allosteric modulator, etc., is bound and results in a change in the protein and/or ligand. For a receptor, the conformation may change, the protein may become susceptible to phosphorylation or dephosphorylation or other processing. For the most part, the active site will be the site(s) of an enzyme where the substrate and/or a cofactor bind, where the substrate and cofactor undergo a catalytic reaction; where two proteins form a complex, e.g. the site at which a G protein binds to a surface membrane receptor, two kringle structures bind, sites at which transcription factors bind to other proteins; or sites at which proteins bind to specific nucleic acid sequences, etc. The skilled artisan will understand that an active site need not be presently performing a catalytic function, but may still bind a TAPP. For example, numerous kinases may bind to adenine nucleotides, but the catalytic function of the kinase may be inhibited due to phosphorylation state, etc. [0047] Structure of TAPPs

[0048] The term "tagged acyl phosphate probes" or "TAPPs" refers to molecules having the following general structure:

O O

TAG- -O-

O-

[0049] wherein TAG is a detectable label, L is a linker moiety covalently bound to the carbonyl through a carbon atom, and X is an affinity moiety for directing the binding of a TAPP to a set of target proteins. A detailed description of a design strategy that can be adapted to the preparation of TAPPs in which a fluorescent moiety can act as a TAG is provided in PCT Application No. PCT/US02/03808, entitled "Activity Based Probe Analysis" (Attorney Docket No. 063391-0202), filed February 5, 2002, PCT Application No. PCT/US00/34187, WO 01/77684, entitled "Proteomic Analysis," and PCT Application No. PCT/US00/34167, WO 01/77668, entitled "Proteomic Analysis," each of which is hereby incorporated by reference in its entirety, including all tables, figures, and claims. Goals of a design strategy are to provide NBAPs that are able to react covalently with a targeted group of nucleotide binding protein(s), while minimizing non-specific labeling. [0050] The term acyl refers to the structure:

O

R-

where the carbonyl carbon is bound to a carbon in R.

[0051] The term "linker moiety" refers to a bond or chain of atoms used to link one moiety to another, serving as a covalent linkage between two or more moieties. Since in many cases, the synthetic strategy will be able to include a functionalized site for linking, the functionality can be taken advantage of in choosing the linking moiety. The choice of linker moiety may alter the specificity of a TAPP. See, e.g., Kidd et al, Biochemistry (2001) 40: 4005-15. For example, an alkylene linker moiety and a linker moiety comprising a repeating alkyleneoxy structure (polyethylene glycols, or "PEG"), have distinct specificities and provide distinct protein profiles. Thus, one of skill in the art can select the linker moiety of the TAPP in order to provide additional specificity of the TAPP for a particular protein or protein class.

[0052] Linker moieties include among others, ethers, polyefhers, diamines, ether diamines, polyether diamines, amides, polyamides, polythioethers, disulfides, silyl ethers, alkyl or alkenyl chains (straight chain or branched and portions of which maybe cyclic) aryl, diaryl or alkyl-aryl groups, having from 0 to 3 sites of aliphatic unsaturation. While normally amino acids and oligopeptides are not preferred, when used they will normally employ amino acids of from 2 - 3 carbon atoms, i.e. glycine and alanine. Aryl groups in linker moieties can contain one or more heteroatoms (e.g., N, O or S atoms). The linker moieties, when other than a bond, will have from about 1 to 60 atoms, usually 1 to 30 atoms, where the atoms include C, N, O, S, P, etc., particularly C, N and O, and will generally have from about 1 to 12 carbon atoms and from about 0 to 8, usually 0 to 6 heteroatoms. The number of atoms referred to above are exclusive of hydrogen in referring to the number of atoms in a group, unless indicated otherwise.

[0053] Linker moieties may be varied widely depending on their function, including alkyleneoxy and polyalkyleneoxy groups, where alkylene is of from 2 - 3 carbon atoms, methylene and polymethylene, polyamide, polyester, and the like, where individual monomers will generally be of from 1 to 6, more usually 1 to 4 carbon atoms. The oligomers will generally have from about 1 to 10, more usually 1 to 8 monomeric units. The monomeric units may be amino acids, both naturally occurring and synthetic, oligonucleotides, both naturally occurring and synthetic, condensation polymer monomeric units and combinations thereof.

[0054] Linker moieties provide a covalent linkage between a TAG and the carbonyl of the acyl group; thus, the final atom of the linker moiety that is covalently linked to the carbonyl must be carbon. A linker moiety may form a branching structure, whereby additional groups, such as a second TAG, may be included in the TAPP structure. [0055] The term "TAG" as used herein refers to a molecule that can be used to detect and/or capture the TAPP in combination with any other moieties that are bound strongly to the TAG, so as to be retained in the process of the reaction of the reactive group with the target active protein. The TAG may be added to the linker moiety combination after reaction of the acyl-nucleotide with the target protein, to form the complete TAPP. For this purpose, the linker moiety will include a chemically reactive group, normally not found in proteins, that will react with a reciprocal functionality on the TAG, e.g. viccinal- diols with boronic acid, photoactivated groups, such as diazo, azide with an alkene or alkyne, o-alkyl hydroxylamine with a ketone or aldehyde, etc. The TAG portion permits capture of the conjugate of the target protein and the TAPP. The TAG may be displaced from the capture reagent by addition of a displacing TAG, which may be free TAG or a derivative of the TAG, or by changing solvent (e.g., solvent type or pH) or temperature or the linker may be cleaved chemically, enzymatically, thermally or photochemically to release the isolated materials (see discussion of the linker moiety, below). [0056] Examples of TAGs include, but are not limited to, detectable labels such as fluorescent moieties and electrochemical labels, biotin, digoxigenin, maltose, ohgohistidine, 2,4-dintrobenzene, phenylarsenate, ssDNA, dsDNA, a polypeptide, a metal chelate, a saccharide, and/or a solid phase. Examples of TAGs and their capture reagents also include but are not limited to: dethiobiotin or structurally modified biotin-based reagents, including deiminobiotin, which bind to proteins of the avidin/streptavidin family, which may, for example, be used in the forms of strepavidin- Agarose, oligomeric-avidin- Agarose, or monomeric-avidin- Agarose; any vicinal diols, such as 1,2-dihydroxyethane (HO-CH₂-CH₂- OH), and other 1,2-dihyroxyalkanes including those of cyclic alkanes, e.g., 1,2- dihydroxycyclohexane which bind to an alkyl or aryl boronic acid or boronic acid esters, such as phenyl-B(OH)₂ or hexyl-B(OEthyl) which may be attached via the alkyl or aryl group to a solid support material, such as Agarose; maltose which binds to maltose binding protein (as well as any other sugar/sugar binding protein pair or more generally to any TAG/TAG binding protein pairs that has properties discussed above); a hapten, such as the dinitrophenyl group, to which an antibody can be generated; a TAG which binds to a transition metal, for example, an oligomeric histidine will bind to Ni(LT), the transition metal capture reagent may be used in the form of a resin bound chelated transition metal, such as nitrilotriacetic acid-chelated Ni(II) or iminodiacetic acid-chelated Ni(II); glutathione which binds to glutathione-S-transferase. In preferred embodiment, the TAGs will be haptens that bind to a naturally occurring receptor, e.g. biotin and avidin, or an antibody or will be a detectable label, that is also a hapten.

[0057] One may use chemical affinity resins, e.g. metal chelates, to allow for digestion of proteins on the solid phase resin and facilitate automation. One example of this is the use of immobilized nickel (II) chelates to purify peptides that have six consecutive histidine residues (His-6 tag) (as described in the Invitrogen product brochureProBond ™ Resin (Purification) Catalog nos. R801-01, R801-15 Version D 000913 28-0076), which could be adapted to include non-peptidic chemical linkage coupling a series of imidazole- containing moieties. Alternative chemical attachments include phenyldiboronic acids (described in Bergseid, M. et al. Biotechniques (2000) 29(5), 1126-1133), and disulfide reagents (described in Daniel, SM et al., Biotechniques (1998) 24(3), 484-489). Additionally, chemical affinity tags that are useful in combinatorial synthesis could be adapted for modified peptide purification (reviewed in Porco, JA (2000) Comb. Chem. High Throughput Screening 3(2) 93-102

[0058] The term "fluorescent moiety" ("FI") refers to a TAG that can be excited by electromagnetic radiation, and that emits electromagnetic radiation in response in an amount sufficient to be detected in an assay. The skilled artisan will understand that a fluorescent moiety absorbs and emits over a number of wavelengths, referred to as an "absorbance spectrum" and an "emission spectrum." A fluorescent moiety will exhibit a peak emission wavelength that is a longer wavelength than its peak absorbance wavelength. The term "peak" refers to the highest point in the absorbance or emission spectrum. [0059] The fluorescent moiety FI may be varied widely depending upon the protocol to be used, the number of different TAPPs employed in the same assay, whether a single or plurality of lanes are used in the electrophoresis, the availability of excitation and detection devices, and the like. For the most part, the fluorescent moieties that are employed as TAG will absorb in the ultraviolet, infrared, and/or most preferably in the visible range and emit in the ultraviolet, infrared, and/or most preferably in the visible range. Absorption will generally be in the range of about 250 to 750 nm and emission will generally be in the range of about 350 to 800nm. Illustrative fluorescent moieties include xanthene dyes, naphthylamine dyes, coumarins, cyanine dyes and metal chelate dyes, such as fluorescein, rhodamine, rosamine, the BODIPY dyes (FL, TMR, and TR), dansyl, lanthanide cryptates, erbium, terbium and ruthenium chelates, e.g. squarates, and the like. Additionally, in certain embodiments, one or more fluorescent moieties can be energy transfer dyes such as those described in Waggoner et al, U.S. Patent no. 6,008,373. The literature amply describes methods for linking fluorescent moieties through a wide variety of linker moieties to other groups. The fluorescent moieties that find use will normally be under 2kDal, usually under lkDal.

[0060] Preferred fluorescent moieties FI can include elaborated conjugated pyran molecules, including xanthenes. Such molecules include eosin, erythrosin, fluorescein,

Oregon green, and various commercially available Alexa Fluor ® dyes (Molecular Probes,

Inc.). Structural examples of such dyes include:

[0061] Particularly preferred fluorescent moieties are the rhodamine dyes. These molecules typically have the general structure:

[0062] Where K is -CO₂H, or -SO₃H; Y is -H, -CH₃, or together with R forms a six- membered ring; Z is -H or together with R forms a six-membered ring; and R is -H, -CH₃, -CH₂CH₃, or together with Y or Z forms a six-membered ring. Rhodamine molecules such as teframethylrhodamine, 5-carboxyteframethylrhodamine, 6-carboxytetramethylrhodamine, carboxyrhodamine-6G, rhodamine-B sulfonyl chloride, rhodamine-red-X, and carboxy-X- rhodamine are well known to those of skill in the art. See, e.g., Handbook of Fluorescent Probes and Research Products, Molecular Probes, Inc., 2001, which is hereby incorporated by reference in its entirety. Advantageous properties of rhodamines include high quantum yields, low sensitivity of fluorescence over a pH range of from about pH 3 to about pH 8, advantageous water solubility, good photostability, and absorption of light in the visible spectrum. Particularly preferred fluorescers are 5-carboxytβtramethylrhodamine and 6- carboxytetramethylrhodamine.

[0063] Other preferred fluorescent moieties FI include the BODIPY dyes, which are elaborations of a 4-bora-3a,4a-diaza--?-indacene structure. Exemplary structures are provided below:

[0064] Yet other preferred fluorescent moieties include the cyanine dyes, conjugated structures comprising a polymethine chain terminating in nitrogen atoms. Typically, the nitrogens are themselves part of a conjugated heterocycle. An exemplary structures is provided below:

[0065] Also of interest for use as TAGs are matched dyes as described in U.S.

Patent No. 6,127,134, which is hereby incorporated by reference in its entirety, including all tables, figures, and claims, which is concerned with labeling proteins with dyes that have different emissions, but have little or no effect on relative migration of labeled proteins in an electrophoretic separation. Of particular interest are the cyanine dyes disclosed therein, being selected in '134 because of their positive charge, which matches the lysine to which the cyanine dyes bind. In addition there is the opportunity to vary the polyene spacer between cyclic ends, while keeping the molecular weight about the same with the introduction of an alkyl group in the shorter polyene chain dye to offset the longer polyene. Also described are the BODffY dyes, which lack a charge. The advantage of having two dyes that similarly affect the migration of the protein would be present when comparing the native and inactived samples, although this would require that in the inactivated sample at least a portion of the protein is monosubstituted. [0066] In each of the foregoing examples of preferred fluorescent moieties, carboxyl groups can provide convenient attachment sites for linker moieties. In the particularly preferred 5- and 6-carboxyrhodamine molecules, the 5- or 6- carboxyl is particularly preferred as an attachment site:

While the following preferred embodiments and exemplified compounds are generally described using only the 5-carboxyrhodamine molecules for the sake of brevity, in each case the 6-carboxyrhodamine version of the indicated molecule, or a mixture of the 5- and 6- carboxyrhodamine molecules should also be considered as an exemplified preferred embodiment.

[0067] In general, any affinity label-capture reagent commonly used for affinity enrichment, which meets the suitability criteria discussed above, can be used in the method of the invention. Biotin and biotin-based affinity TAGs are particularly illustrated herein. Of particular interest are structurally modified biotins, such as deiminobiotin or dethiobiotin, which will elute from avidin or streptavidin (strept/avidin) columns with biotin or under solvent conditions compatible with ESI-MS analysis, such as dilute acids containing 10-20% organic solvent. For example, deiminobiotin tagged compounds will elute in solvents below about pH 4. [0068] In certain embodiments, TAPPs can be immobilized on a solid phase to form a "tethered" TAPP in which the TAG is represented by the solid phase. In preferred embodiments, a plurality of different TAPPs may be tethered to different regions of one or more solid phases to form a patterned array. Such a patterned array having two or more regions comprising TAPPs that differ in structure and/or reactivities from each other could be used to simultaneously measure the presence, amount, or activity of a plurality of target nucleotide binding proteins. The term "solid phase" as used herein refers to a wide variety of materials including solids, semi-solids, gels, films, membranes, meshes, felts, composites, particles, and the like typically used by those of skill in the art to sequester molecules. The solid phase can be non-porous or porous. Suitable solid phases include those developed and/or used as solid phases in solid phase binding assays. See, e.g., chapter 9 of Immunoassay, E. P. Diamandis and T. K. Christopoulos eds., Academic Press: New York, 1996, hereby incorporated by reference. Examples of suitable solid phases include membrane filters, cellulose-based papers, beads (including polymeric, latex, glass, and paramagnetic particles), glass, silicon wafers, microparticles, nanoparticles, TentaGels, AgroGels, PEGA gels, SPOCC gels, and multiple-well plates. See, e.g., Leon et al., Bioorg. Med. Chem. Lett. 8: 2997 (1998); Kessler et al., Agnew. Chem. frit. Ed. 40: 165 (2001); Smith et al., J. Comb. Med. 1: 326 (1999); Orain et al., Tetrahedron Lett. 42: 515 (2001); Papanikos et al., J. Am. Chem. Soc. 123: 2176 (2001); Gottschling et al., Bioorg. And Medicinal Chem. Lett. 11: 2997 (2001).

[0069] The specificity and affinity of a TAPP may be affected by the choice of the affinity moiety, the linker moiety, the TAG, or a combination thereof. In certain embodiments, the affinity moiety X may be deleted; in these embodiments, L can provide an affinity moiety either inherently in its own structure, or by means of a branched L linking both a TAG and a separate affinity moiety. One or more TAPPs may be designed that exhibit specificity for a single target protein, or that exhibit specificity for a plurality of targets that may be structurally or functionally related.

[0070] TAPPs of the present invention may comprise any affinity moiety that directs a TAPP to target proteins of interest. Suitable affinity moieties include small molecules, such as combinatorial libraries or therapeutic lead compounds; hormones, such as steroids, peptide hormones, etc.; cofactors; vitamins; enzyme substrates; lipids; prostaglandins; receptor ligands; nucleotides and nucleotide analogues, optionally substituted naphthyl groups, etc. As used herein, the term "small molecule" refers to compounds having molecular mass of less than 3000 Daltons, preferably less than 2000 or 1500, still more preferably less than 1000, and most preferably less than 600 Daltons. Exemplary alternative affinity moieties are shown in Fig. 5. All that is required of an affinity moiety is that it comprises an available alcohol for attachment of the acyl phosphate; or an available carbon atom for attachment of the acyl phosphonate. [0071 ] Exemplary acyl nucleotide NB APs

[0072] Exemplary TAPPs described in detail below are those in which the affinity moiety X is selected to provide an acyl-nucleotide structure. Referred to herein by the term "nucleotide binding protein-directed affinity probes" ("NBAPs"), these preferred TAPPs comprise a nucleotide or nucleotide anlogue covalently bound through the terminal phosphate of a 5' mono- di- or tri-phosphate (or 2' or 3' mono-, di-, or tri-phosphate) to an acyl group, which is itself further covalently bound to a TAG via a linker moiety. [0073] The term "nucleotide" as used herein refers to a purine or pyrimidine base linked glycosidically to ribose, 2' or 3' deoxyribose, or 2',3' dideoxyribose; and which comprise a 5' mono- di- or tri-phosphate. Preferred bases include adenine, thymine, uracil, guanine, cytosine, and inosine. Nonnaturally occurring bases such as 5-bromouracil, 5- fluorouracil, 2-aminopurine, N⁶-cyclohexyl adenine, l^-ethenoadenosine; 8-azaguanine, and 5-fluorocytosine are also well known in the art. This list is not meant to be limiting, and any purine or pyrimidine base is within the scope of the present invention. The general structure of nucleotides is as follows:

nucleotide monophposphate

nucleotide diphposphate

nucleotide triphposphate

[0074] where R > and R₃> are independently H or OH, and where BASE is a purine or pyrimidine.

[0075] The term "nucleotide analogue" as used herein refers to a nucleotide-like structure in which the purine or pyrimidine BASE is replaced with a non-purine or non- pyrimidine structure (e.g., substituted or unsubstituted triazine, pyridazine, pyrazine, pyrrolopyrimidine, or pyrrazolopyrimidine); in which the ribose is replaced with a non- ribose structure; in which the oxygen lying between adjacent phosphates is replaced (e.g., with NH, S, or methylene); in which R_2' and R₃> are other than H or OH or in which the phosphate moiety or moieties is at the R₂> or R₃> position; and which binds to a nucleotide binding site of at least one nucleotide binding protein. See, e.g., U.S. Patents 6,255,292; 6,043,060; and 5,215,970.

[0076] The term "BASE" as used herein refers to a 5- or 6-membered unsaturated heterocyclic ring comprising from 1 to 3 nitrogen heteroatoms; attached through a ring heteroatom to the 1' position of a ribose, wherein the 5- or 6-membered heterocyclic ring may comprise a 6-membered unsaturated carbocyclic or heterocyclic ring comprising from 1 to 2 nitrogen heteroatoms. Each carbon position in the BASE may be optionally substituted by a substituent independently selected from the group consisting of -H, -F, -Br, -CI, -SCH₃, -C(O)N(R)(R), -CN, -NO₂, -N(R)(R), =O, acetoxy, -C(R)(R)(R), -OCH₃, - OCH₂CH₃, methylene dioxy, trihalomethyl, trihalomethoxy, or -(CH₂)_nOH, where each R is independently H or -Cι-₆ alkyl straight or branched chain, and n is 0-6. Exemplary BASE structures are shown in Fig. 4.

[0077] In preferred embodiments, a nucleotide or nucleotide analogue of the present invention comprises a base (preferably a substituted or unsubstituted purine or pyrimidine) linked glycosidically to ribose, and R _' and R_3' are independently selected from the group consisting of -H, -OH, -F, -Br, -CI, -SCH₃, -C(0)N(R)(R), -CN, -NO₂, -N(R)(R), benzoyl, benzoylbenzoyl, azido, acetoxy, -C(R)(R)(R), -OCH₃, -OCH₂CH₃, methylene dioxy, trihalomethyl, trihalomethoxy, -(CH₂)_nOH, or -(CH₂)_n-phenyl where phenyl is optionally substituted with -F, -Br, -CI, -SCH₃, -C(O)N(R)(R), -CN, -NO₂, -N(R)(R), acetoxy, - C(R)(R)(R), -OCH₃, -OCH₂CH₃, methylene dioxy, trihalomethyl, trihalomethoxy, - (CH₂)_nOH; where each R is independently H or -Cι-₆ alkyl straight or branched chain, or optionally form an optionally substituted fused carbocyclic or heterocyclic ring structure, and n is 0-6, or where one of R₂> and R₃. comprises a phosphate moiety or moieties, e.g., a mono-, di-, or tri-phosphate moiety as is linked at the ribose 5 '-position in conventional nucleotide mono-, di-, and tri-phosphates respectively as illustrated above. [0078] In preferred embodiments, the NBAP(s) of the present invention have one of the following general formulae:

[0079] Preferably, each R > and R₃> is independently selected from the group consisting of -H, -OH, -F, -Br, -CI, -SCH₃, -C(O)N(R)(R), -CN, -NO₂, -N(R)(R), acetoxy, - C(R)(R)(R), -OCH₃, -OCH₂CH , methylene dioxy, trihalomethyl, trihalomethoxy, - (CH )_nOH, or -(CH₂)_n-phenyl where phenyl is optionally substituted with -F, -Br, -CI, - SCH₃, -C(0)N(R)(R), -CN, -NO₂, -N(R)(R), acetoxy, -C(R)(R)(R), -OCH₃, -OCH₂CH₃, methylene dioxy, trihalomethyl, trihalomethoxy, -(CH₂)_nOH; and each R_2' and Ry are most preferably independently H or OH;

[0080] each Z is independently O, S, NH, or methylene;

[0081] n is between 0 and 6 inclusive;

[0082] BASE is a substituted or unsubstituted purine, pyrmidine, triazine, pyridazine, pyrazine, pyrrolopvrimidine, orpyrrazolopyrimidine, and is most preferably selected from the group consisting of include adenine, thymine, uracil, guanine, cytosine, and inosine; [0083] TAG is a detectable label or solid phase;

[0084] L is an optionally present alkyl or heteroalkyl groups of 1-40, 1-30, or 1-20 backbone atoms selected from the group consisting of -N(R)-, -O-, -S- or -C(R)(R)-, which may include a carbocyclic or heterocyclic moiety, e.g., a triazole ring; and

[0085] each R is independently H or -Cι-₆ alkyl straight or branched chain, or optionally form an optionally substituted fused carbocyclic or heterocyclic ring structure.

[0086] In certain embodiments, the NBAP(s) are as described for the immediately preceding structure, except that the moiety shown above attached at the ribose 5' carbon is instead attached at R_2' or Ry, and is replaced at the ribose 5' carbon with a group R₅<. Ry is selected from the group consisting of -H, -OH, -F, -Br, -CI, -SCH₃, -C(O)N(R)(R), -CN, -

NO₂, -N(R)(R), acetoxy, -C(R)(R)(R), -OCH₃, -OCH₂CH₃, methylene dioxy, trihalomethyl, trihalomethoxy, -(CH₂)_nOH, or -(CH₂)_n-phenyl where phenyl is optionally substituted with -

F, -Br, -CI, -SCH₃, -C(O)N(R)(R), -CN, -NO₂, -N(R)(R), acetoxy, -C(R)(R)(R), -OCH₃, -

OCH CH₃, methylene dioxy, trihalomethyl, trihalomethoxy, -(CH )_nOH; and is most preferably H or OH.

[0087] The person of ordinary skill will realize that pharmaceutically acceptable salt or complexes of these compounds are also useful and are also contemplated within the scope of the invention. Exemplary purine and pyrimidine-based NBAPs are shown in Fig. 1.

[0088] A preferred group of linking moieties L fall within the following formulae:

where n and m are independently in the range of 0 to 4, and X is O or CH₂;

o o

I ■

TAG NH(CH₂)o-₄(CH₂CH₂)o-₄NHC(CH₂)₂-₁₀"C ; or

O 0 0

TAG NH(CH₂)o-₄(CH₂CH₂)o-₄NHC(CH₂)₂-₁₀C C

[0089] In particularly preferred embodiments, L is -NH(CH₂)₂(OCH₂CH )ι-₄-.

[0090] Another preferred group of linkers are those that can be formed using

"click"chemistry", such as triazole linkers. The use of such click chemistry in the preparation of certain activity-based probes is described in Shreder et al., International Application PCT/US03/07898, WO 03/079014, which is incoφorated herein by reference in its entirety, including drawings. Additional useful descriptions of "click chemistry" are available, for example, in Kolb et al, Agnew Chem. Lnt. Ed. Engl. 40: 2004-21 (2001); Seo et al, J. Org. Chem. 68: 609-12 (2003), both of which are incoφorated herein in their entireties.

[0091] An exemplary triazole linker moiety formed using "click chemistry" is shown below. The first structure shows the linker extending to the nitrogens that further link the dye and the acyl phosphate/affinity moieties. The second structure is focused on the formation of the triazole ring, for example, using an azide/alkyne reaction. [0092] Another example of ligation chemistry that has been applied to proteomic samples and is useful in forming the present probes is the Staudinger reaction between a phosphine and an azide (Bertozzi et al. J. Am. Chem. Soc. 125: 4708-4709 (2003)) which is incoφorated herein by reference in its entirety. In this reaction a stable amide bond is formed between the two components. The reaction is illustrated below, where Ph stands for phenyl.

Click Chemistry

Staudinger Reaction

[0093] Thus, typically a linker resulting from such a Staudinger reaction will contain the following structure:

[0094] The "click chemistry" and Staudinger reaction allow convenient ligation in aqueous solutions.

[0095] TAGs of particular interest come within the following formulae:

where the exemplified 5-carboxyrhodamine or 5-carboxyffuorescein may also be the equivalent 6-substituted molecule or a mixture of 5- and 6-substituted molecules.

[0096] Analysis of samples with TAPPs

[0097] After the reaction between the complex protein mixture and the TAPP(s) is completed, the conjugates of the TAPP(s) and protein targets will be analyzed. Preferably, the TAPPs of the present invention comprise a TAG that allows for manipulation of the conjugates, either for sequestering the conjugates or detecting the conjugates or both. The TAPPs may be analyzed by separating into components, e.g., by electrophoresis, for example gel electrophoresis, capillary electrophoresis or microfluidic electrophoresis; mass spectrometry, e.g., MALDI-TOF, microcapillary liquid chromatography-electrospray tandem MS, or other technique. To enhance the analysis, the conjugates may be deglycosylated using an appropriate glycosidase, such as PGNaseF, under conventional deglycosylation conditions indicated by the enzyme supplier. Labeled target proteins can be identified based on a variety of physical criteria, such as apparent molecular weight, peptide sequence composition, enzymatic activity (e.g., kinase activity), or a combination of such criteria.

[0098] The term "separating" as used herein refers to methods that enrich the concentration of a molecule of interest in a particular location or container relative to other molecules originally present. For example, gel electrophoresis enriches the concentration of molecules that migrate at a particular rate relative to other molecules originally present that migrate at different rates; sequestration methods enrich the concentration of molecules capable of being sequestered (e.g., by binding to a receptor) relative to other molecules not so capable (e.g., removed by washing out molecules that do not bind to a receptor). Numerous additional analytical procedures are known to the artisan for separating and analyzing complex protein mixtures (e.g., chromatographic methods such as HPLC, FPLC, ion exchange, size exclusion; mass spectrometry; differential centrifugation). [0099] In preferred embodiments, the TAPP-labeled products are analyzed by electrophoresis, e.g., slab gel, capillary or microfluidic, optionally using a gel for separation of the different components. In particularly preferred embodiments, SDS-PAGE is used, including 2D PAGE. The sample composition may be preliminarily separated using isoelectric focusing, followed by using bands or regions for further electrophoretic separation. Conventional conditions can be employed for the electrophoresis, using a denaturing medium, so that the active sample and the inactivated sample are both denatured in the gel. Numerous patents have issued for performing electrophoresis for the separation of proteins. See, e.g., U.S. Patent Nos. 4,415,655; 4,481,094; 4,865,707; and 4,946,794. Texts describing procedures include Laemmli, Nature 227:680-685 (1970); Sambrook et al., "Molecular Cloning: A Laboratory Manual." 3^rd Edition, Cold Spring Harbor Press, Cold Spring Harbor, NY. (2001).

[00100] Using the TAPPs of the present invention, labeled target protein(s) may be identified by excitation and detection of light emitted upon excitation of the fluorescent moiety, e.g., in electrophoresis gels. In certain embodiments, such as when the TAPP labels a plurality of target proteins or when the identity of a labeled target protein is imknown, the labeled target protein(s) present in various electophoretic bands may be further assayed to identify the specific proteins to which the TAPP(s) bound, e.g., by fragmentation and mass spectrometric analysis. In particular, the sequence of proteins can be determined using tandem MS (MSⁿ) techniques. By application of sequence database searching techniques, the protein from which a sequenced peptide originated can be identified. Exemplary methods for performing such analyses are described in U.S. Patent Application No. 60/446,960, entitled "Macromolecule Identification Made by Mass Spectroscopy and Database Searching," filed February 11, 2003, Atty Docket No. 11267-003-888, which is hereby incoφorated by reference in its entirety, including all tables, figures, and claims. [00101] In designing a gel-based analysis system, the artisan may balance various considerations, such as speed, resolution, sample volume, choice of fluorophore, detection methods, etc., in order to arrive at an optimal solution. For example, for simple screening analysis (i.e., when gel bands are not to be identified by means of eluting proteins from the gel matrix for further analysis), very thin gels may be run quickly. Additionally, such thin gels are amenable to the use of laser-induced fluorescence scanning systems and narrow gel lanes, as laser focusing and confocal detection optics permit the detection of very small amounts of TAPP-labeled protein in a sample. Conversely, thicker gels may be advantageous in protein identification analysis, as a sufficient amount of material must be obtained from a gel band to permit further manipulations.

[00102] For rapid screening analysis, a suitable gel electrophoresis platform would consist of a glass sandwich gel format of from 15-40 cm in width, 20-40 cm in length, and from 0.6 to 0.2 cm in thickness. A partciularly preferred format is from about 30-35 cm in width, about 25-30 cm in length, and about 0.4 mm in thickness. The term "about" in this context refers to +/- 10% of a given dimension. The gel format is preferably combined with a laser-induced fluorescence detector apparatus comprising detection optics that permit sampling of the gel without removal from the gel plates, as such thin gels may be extremely fragile. Typically, such an instrument uses confocal optics for detection. By matching the thickness of the gel to the thickness of the confocal "slice," signal detection can be matched to a minimal amount of sample.

[00103] The spacing between sample wells is limited only by the amount of sample necessary to obtain a sufficient signal for measurement. Appropriate spacings are between 1 and 4 mm, most preferably about 2.25-3 mm. The term "about" in this context refers to +/- 10% of the spacing between wells. Selecting a spacing between wells of about 2.25 mm as an example, a gel platform 25 cm in width could accommodate as many as 96 individual samples.

[00104] After completing the electropherogram, the bands may then be read using any convenient detection means (e.g., a fluorescent reader, e.g., Hitachi FMbio Flatbed Fluorescence Scanner, when the TAPP comprises a fluorescent moiety), where the intensity of each band may be transferred to a data processor for processing. Depending on whether one or more lanes are involved with the analysis, the data may be compiled from a single or multiple lanes to establish the bands associated with active target proteins that are absent with the inactive sample, the different target proteins that reacted with different TAPPs as evidenced by the different fluorescence emission for each of the TAPPs, and any cross- reactivity between the TAPPs. The bands that are obtained in the gel are shaφ and provide for excellent resolution. Particularly, much better resolution and sensitivity may be obtained than when biotin-labeled TAPPs are used, followed by complex formation with labeled avidin, and Western blotting.

[00105] The results obtained from analyzing the nucleotide binding protein profiles may then be organized in a manner that allows for ready comparisons and differentiation between samples. One technique that finds utility is cluster analysis. One applies a hierarchical clustering algorithm to the samples using the Pearson correlation coefficient as the measure of similarity and average linking clustering (Cluster program: Ross et al., Nat. Genet. 24:227-35 (2000); Eisen et al., Proc. Natl. Acad. Sci. USA 95:14863-68 (1998)). For each enzyme activity, averaged cell sample values are compared to identify the cell sample that expressed the highest level of a particular enzyme activity. The activity levels may then be expressed as a percentage of this highest activity to normalize the data sets. As data sets are built up from cell samples, the cluster analysis can be modified in light of new data that provides a new maximum for a particular enzyme, so that one may have cluster analysis within a given group of samples as well as cluster analysis extending over many samples and groups of samples. Cluster analysis can also be applied as to the individual fractions and pair-wise combinations, so as to maximize information from the cell samples in relating the samples to each other and standards. For large numbers of samples, clustergrams can be used to rapidly identify the similarities between samples, for example, in terms of origin of the cells, aggressiveness and invasiveness, diagnosis, prognosis, preferential therapies and how the tumor has responded to a course of treatment.

[00106] Following TAPP labeling of target nucleotide binding protein(s), protein digestion may be employed to produce both unlabeled and TAPP-labeled peptides. The denaturant, and/or to provide suitable buffer conditions for digestion. In particularly preferred embodiments, buffer exchange is performed by gravity flow gel filtration. [00109] Digestion will be carried out in an aqueous buffered medium, generally at a pH in the range of about 4 to 10, depending on the requirements of the protease. The concentration of the protease will generally be in the range of about 6 x 10^"8 M to about 6 x 10^"6 M, more preferably in the range of about 1.8 x 10^"8 M to about 2 x 10^"7 M, and most

preferably about 6 x 10^"7 M (e.g., 150 ng / 10 μL). The term "about" in this context means

+/- 10%) of a givem measurement. The time for the digestion will be sufficient to go to at least substantial completion, so that at least substantially all of the protein will have been digested. Digests may be performed at a temperature that is compatible with the protease(s)

employed, preferably from 20°C to 40°C, most preferably about 37°C. Where the digestion

takes place in solution, the protease may be quenched by any convenient means, including heating or acidification of the sample. Alternatively, quenching can be achieved by sequestering the fragment conjugates with a receptor for the TAG bound to a surface, or by addition of a protease inhibitor (e.g., E64, DIFP, PMSF, etc.). Where the proteins are bound to a surface, the proteases may be washed away before the bound digested protein is released.

[00110] Following protein digestion, peptides can be sequestered, e.g., by binding to receptors for the TAG of one or more TAPP-labeled peptides. Preferably, sequestration relies on receptors bound to a solid support that can be easily manipulated during wash steps. The support may be beads, including paramagnetic beads, prepared from various materials, such as Bioglas, polystyrene, polyacrylate, polymethylmethacrylate, polyethylene, polysaccharides, such as Agarose, cellulose, amylose, etc., polyurethane, and the like. Desirably, the support surface will not interfere with the binding of TAG to its cognate receptor, and the receptor may be linked to the support by a hydrophilic bridge that

40 digestion may be performed while the proteins are in solution or when the conjugates are sequestered, e.g., by receptors bound to a solid support. Digestion preferably employs only one protease; however, two or more, usually not more than three, proteases may be used. The proteases may be in solution or bound to a surface. The proteases may be combined in the same reaction mixture, or the sample may be divided into aliquots and each of the aliquots treated with a different protease. Digestion may also occur before binding to the conjugate to a support and/or a after the conjugates are bound to a solid support. Enzymes that find use include, but are not limited to, trypsin, chymotrypsin, bromelain, papain, carboxypeptidase A, B and Y, proteinase A and K, chymopapain, plasmin, subtilisin, clostripain etc.

[00107] In particularly preferred embodiments, additional steps can be used to reduce the complexity of the analysis to be performed. For example, the complex protein mixture can be denatured following labeling, e.g., by the addition of urea, guanidinium salts, detergents, organic solvents, etc., in order to reduce or eliminate unwanted proteolysis from endogenous proteases present in the mixture. Additionally, cysteine residues can be reduced and alkylated to maintain the homogeneity of cysteine-containing peptides and to prevent refolding of endogenous proteases following removal of the denaturant. Moreover, proteases can be combined with additional enzymes, such as glycosidases, phosphatases, sulfatases, etc., that can act to remove post-translational modifications from proteins. Examples of such post-translational modifications include, but are not limited to, glycosylations, phosphorylations, sulfations, prenylations, methylations, amidations, and myristolations. Such steps can be mixed and matched by the skilled artisan, depending on the requirements of a particular analysis.

[00108] Prior to digestion, a buffer exchange step may be employed, e.g., by gel filtration, dialysis, etc. This step may be used to remove excess TAPPs, to remove

39 allows for the receptor to be removed from the surface. When beads are employed, the

beads will generally have a cross-dimension in the range of about 5 to lOOμm. Instead of

beads, one may use solid supports, such as slides, the walls of vessels, e.g. microtiter well walls, capillaries, etc. There is an extensive literature of receptor bound supports that is readily applicable to this invention, since the sequestering step is conventional. The sample is contacted with the support for sufficient time, usually about 5 to 60 min, to allow all of the conjugate to become bound to the surface. At this time, all of the non-specifically bound components from the sample may be washed away, greatly enriching the target proteins as compared to the rest of the sample.

[00111] Following separation by sequestration, TAPP-labeled peptides may then be released from the receptor. The particular method of release will depend upon the TAG- receptor pair. In some instances, one may use an analog of the TAG as a "releasing agent" to release the conjugate. This is illustrated by the use of deimino- or dethiobiotin as the TAG and biotin as the releasing agent. Where this is not convenient, as in the case of many fluorescent moieties as TAGs where there may not be a convenient analog, conditions such as high salt concentrations, chaeotropic agents (e.g., isothiocyanate or urea) low pH, detergents, organic solvents, etc., may be used to effect release. Once the conjugate has been released, dialysis, ion exchange resins, precipitation, or the like may be used to prepare the conjugate solution for the next stage.

[00112] Where the migration rates in various separation procedures provide the necessary identification of the peptide(s) generated and, therefore, the protein from which they are obtained, no further analysis may be required. However, where further identification is desired or the earlier results do not provide certainty as to the identification and amount of a particular component, an identification method using mass spectrometry (MS) can be employed. See, for example, WO 00/11208. The use of mass spectrometry will be described below. Such identification methods potentially provide greater information, but requires greater sample size in comparison to, for example, capillary elecfrohoresis, and has a lower throughput.

[00113] Chromatographic and/or electrophoretic separation methods as described herein may be used to simplify the mixtures introduced into the mass spectrometer, allowing for a more accurate analysis. For TAPP-labeled peptides, the use of fluorescent moieties as TAPP TAGs can permit the use of an online fluorescence detector to trigger ESI-MS data collection or fraction collection for subsequent analysis, e.g., providing sample on a MALDI plate. In this way, only fractions and bands that contain TAPP-labeled peptides will be selected for further processing, thereby avoiding using the MS with certain fractions.

[00114] In particularly preferred embodiments, the identification methods described herein can be combined with one or more separation methods to develop a "separation profile" that can be used to identify peptides without the need for MS analysis. In these methods, a sample (e.g., material from a chromatography column) is divided into at least two portions; one portion is used for MS analysis, and the other portion(s) are used for one or more separation methods (e.g., a single CE run, or two or more CE runs using different separation conditions). The peptide identification obtained from the MS analysis can be assigned to the observed separation profile (e.g., the elution time of the peptide observed in the CE run(s)). Observation of this separation profile in subsequent samples can then be correlated to the peptide known to exhibit that separation profile. [00115] The identification methods described herein may also utilize TAPPs that differ isotopically in order to enhance the information obtained from MS procedures. For example, using automated multistage MS, the mass spectrometer may be operated in a dual mode in which it alternates in successive scans between measuring the relative quantities of peptides obtained from the prior fractionation and recording the sequence information of the peptides. Peptides may be quantified by measuring in the MS mode the relative signal intensities for pairs of peptide ions of identical sequence that are tagged with the isotopically light or heavy forms of the reagent, respectively, and which therefore differ in mass by the mass differential encoded with the TAPP. Peptide sequence information may be automatically generated by selecting peptide ions of a particular mass-to-charge (m/z) ratio for collision-induced dissociation (CID) in the mass spectrometer operating in the MSⁿ mode. (Link, et al., (1997) Elecfrophoresis 18:1314-34; Gygi, et al., (1999) idid 20:310-9; and Gygi et al., (1999) Mol. Cell. Biol. 19:1720-30). The resulting CTD spectra may be then automatically correlated with sequence databases to identify the protein from which the sequenced peptide originated. Combination of the results generated by MS and MS" analyses of affinity tagged and differentially labeled peptide samples allows the determination of the relative quantities as well as the sequence identities of the components of protein mixtures.

[00116] Protein identification by MSⁿ may be accomplished by correlating the sequence contained in the CID mass spectrum with one or more sequence databases, e.g., using computer searching algorithms (Eng. et al. (1994) J. Am. Soc. Mass Spectrom. 5:976- 89; Mann, et al., (1994) Anal. Chem. 66:4390-99; Qin, et al., (1997) ibid 69:3995-4001; Clauser, et al., (1995) Proc. Natl. Acad. Sci. USA 92:5072-76); see also, U.S. Patent Application No. 60/446,960, entitled "Macromolecule Identification Made by Mass Spectroscopy and Database Searching," filed February 11, 2003, Atty Docket No. 11267- 003-888. Pairs of identical peptides tagged with the light and heavy affinity tagged reagents, respectively (or in analysis of more than two samples, sets of identical tagged peptides in which each set member is differentially isotopically labeled) are chemically identical and therefore serve as mutual internal standards for accurate quantitation. The MS measurement readily differentiates between peptides originating from different samples, representing different cell states or other parameter, because of the difference between isotopically distinct reagents attached to the peptides. The ratios between the intensities of the differing weight components of these pairs or sets of peaks provide an accurate measure of the relative abundance of the peptides and the conelative proteins because the MS intensity response to a given peptide is independent of the isotopic composition of the reagents. The use of isotopically labeled internal standards is standard practice in quantitative mass spectrometry (De Leenheer, et al., (1992) Mass Spectrom. Rev. 11:249- 307).

[00117] The following examples are offered by way of illustration and not by way of limitation.

[00118] fri the following examples, 1H-NMR spectra were recorded using deuterated

DMSO as the solvent unless otherwise indicated. Preparative HPLC was carried out on a

reverse phase Polaris ₈ column (5 μ column; 150 mm x 21 mm; Metachem/Ansys;

Torrance, CA) using a binary system of water and acetonitrile with TFA as a modifier (water 0.1%>, acetonitrile 0.1%). Analytical LC-MS was carried out on a Polaris C18

column (5 μ column; 50 mm x 4.6 mm; Metachem Ansys; Torrance, CA) using a binary

system of water and acetonitrile with TFA as a modifier (water 0.1%, acetonitrile 0.1%). All compounds were obtained from the Aldrich Chemical Company (Milwaukee, WI) unless indicated otherwise. Fmoc-4-(aminomethyl)benzoic acid was obtained from Advanced ChemTech (Louisville, Kentucky); the mixed 5- and 6-succinimidyl ester of teframethylrhodamine was obtained from Molecular Probes (TAMRA-SE; Eugene, OR); and fluoroacetyl fluoride was obtained from ProChem, Lie (Rockford, IL). [00119] Example 1 - Preparation of acyl-nucleotide NBAPs

[00120] Exemplary general reaction schemes for the formation of acyl-nucleotide monophosphate NBAPs; and for the formation of acyl-nucleotide diphosphate and triphosphate NBAPs; are shown in Figs. 2 and 3, respectively. Specific exemplary reaction schemes follow in the following examples. [00121] Example 2: TAMRA-6'-NH-(CH₂)ι₀-COOH (1):

[00122] To a stirred solution of TAMRA acid (2.5 g, 5.8 mmole), DMAP (781 mg,

6.4 mmole) in dry DMF (22 ml) was added disuccinimidyl carbonate (1.64 g, 6.4 mmole) at room temperature. The resulting red solution was stirred at that temperature for four hours. HPLC analysis showed that TAMRA-SE was formed in over 90% yield. In another flask was added 11-aminoundecanoic acid (1.17 g, 5.8 mmole), bis(trimethylsilyl)acetamide and DMF (6 ml), the suspension was heated with a heat-gun until a clear solution appeared. The flask was allowed to cool to room temperature and stirred for one hour before transferring the solution into the flask containing the TAMRA-SE. The resulting mixture was stirred overnight before it was quenched with a few drops of acetic acid and water. The mixture was concentrated and purified by flash column chromatography (SiO₂, 45 x 260 mm, gradient 10% MeOH / CH₂C1₂ / 1% AcOH to 20% MeOH / CH₂C1₂ / 1% AcOH) to give compound 1 as a red solid (608 mg, 17%> yield, 5 '-isomer of compound 1 was also obtained along with fractions containing both 5'- and 6'- isomers), compound 1 can be further purified by HPLC. 1H-NMR (400MHz, DMSO-d₆) δ 8.72 (t, IH, CONH), 8.28 (d, J = 8.0

Ηz, 1Η, aromatic proton), 8.24 (d, J = 8.0 Ηz, 1Η, aromatic proton), 7.87 (s, 1Η, aromatic proton), 7.04 (m, 4Η, aromatic protons), 6.93 (m, 2H, aromatic protons), 3.24 (m, 2H, CONHCH₂), 3.24 (s, 6Η, NCH ), 2.10 (t, J = 7.4 Ηz, 2Η, CH₂COOH), 1.42 (m, 4H, NHCH₂CH₂, CH₂CΗ₂COOΗ), 1.18 (m, 12H, CH₂); LRMS (ESI, [M + Η⁺]) calculated for C₃₆H₄₃N₃O₆: 614; found: 614. [00123] Example 3: TAMRA-dAMP acylphosphates (2) and (3):

[00124] In a NMR tube fitted with a cap was added 1,3-diisopropylcarbodiimide

(12.4 μl, 0.08 mmole) to a solution of l (9.7 mg, 0.016 mmole) in pyridine (400 μl). The

resulting red mixture was kept at room temperature for ten minutes before a solution of 2'- deoxyadenosine 5'-monophosphate (5.2 mg, 0.016 mmole) in D₂O/Pyridine (10: 1, 110 μl)

was added. The reaction was monitored by ³¹P-NMR and quenched by water (2 ml) after 25 minutes. The mixture was extracted with EtOAc (2x2 ml). The aqueous layer was lyophilized. The resulting red solid was dissolved in a mixture of DMSO / H₂O (1:1, 2ml),

filtered and purified by a l50 x 21.2 mm Polaris 5 μ C18-A column (MetaChem) at a flow

rate of 20 ml/min with a gradient of 0.1% TFA / 2% CH₃CN / H₂O to 0.1% TFA / 100% CH₃CN over 30 min. The fractions were collected at 550 nm. The compounds 2 (RT = 21.4 min) and 3 (RT = 22.1 min) were obtained along with a side product and the hydrolyzed

starting material. 2: 1H-NMR (400MHz, DMSO-d₆) δ 8.75 (t, IH, CONH), 8.50 (s, 1Η), 8.25 (m, 2Η), 8.22 (s, IH), 7.87 (s, IH), 7.03 (m, 4H), 6.95 (m, 2H), 6.35 (t, IH, H-1'), 4.40 (m, IH), 3.97 (m, 2H), 3.26 (s, 6H, NCH,), 3.00 (m, 2Η, CONHCH₂), 2.70 (m, 2Η), 2.29 (m, 2H, CH₂COOH), 1.49 (m, 4H, NHCH₂CH₂, CH₂CΗ₂COOΗ), 1.19 (m, 12H, CH₂); ³¹P-

NMR (162 MHz, DMSO-d₆) δ -7.92 (s, IP). 3: 1H-NMR (400MHz, DMSO-d₆) δ 8.73 (t,

IH, CONH), 8.47 (s, 1Η), 8.28 (m, 3Η), 7.86 (s, IH), 7.05 (m, 4H), 6.91 (m, 2H), 6.34 (t, IH, H-1'), 4.25 (rn, IH), 3.86 (m, 2H), 3.24 (m, 6H, NCH_?), 2.98 (m, 2Η, CONHCH₂), 2.29 (m, 2Η), 2.16 (t, J = 7.2 Hz, 2H, CH₂COOH), 1.48 (m, 4H, NHCH₂CH₂, CH₂CΗ₂COOΗ),

1.22 (m, 12H, CH₂); ³¹P-NMR (162 MHz, DMSO-d₆) δ -7.62 (s, IP).

[00125] Example 4: Synthesis of TAMRA- AMP aeylphospiiate (4)

(4)

[00126] This compound was prepared using the procedure described for 2 and 3. H-

NMR (400MHz, DMSO-d₆) δ 9.45-7.80 (m, 4H), 7.55-7.00 (m, 6H), 7.10-6.10 (m, 2H), 6.00-4.55 (m, 2H), 4.80-3.30 (m, 18H), 3.05-2.80 (m, 6H), 2.55-2.45 (m, IH), 2.00-1.55 (m,

7H), 2.70 (m, 2H), 0.60-0.50 (m, 2H); ³¹P-NMR (162 MHz, DMSO-d₆) δ -7.97 (s, IP).

[00127] Example 5: TAMRA-6'-NH-(CH₂)ιo-l-Nap-Acylphosphate (5):

[00128] This compound was prepared using the same procedure as for 2 and 3 with one exception, HPLC purification was run with a gradient of 2% CH₃CN / H₂0 to 100%

CH₃CN: 1H-NMR (400MHz, DMSO-d₆) δ 8.75 (t, IH, CONH), 8.18 (m, 2Η), 7.95 (d, J =

8.8 Hz, IH), 7.85 (d, J = 8.4 Hz, IH), 7.82 (s, IH), 7.49 (m, 2H), 7.42 (m, IH), 7.23 (m, IH), 7.12 (m, IH), 6.96 (m, 4H), 6.79 (m, 2H), 3.24 (m, 2H, CONHCH₂), 3.22 (s, 6Η, NCH₃), 2.16 (t, J = 7.4 Ηz, 2Η, CH₂COOH), 1.47 (m, 4H, NHCH₂CH₂, CH₂CΗ₂COOΗ),

1.23 (m, 12H, CH₂); ³¹P-NMR (162 MHz, DMSO-d₆) δ -13.62 (s, IP).

[00129] Example 6: (+)-Biotin-Acyl-AMP (6)

(6) [00130] In a NMR tube fitted with a cap was added (+)-biotin (6.9 mg, 0.03 mmole),

pyridine/DMF (8:1, 440 μl) and 1,3-diisopropylcarbodiimide (22.0 μl, 0.14 mmole). The

resulting mixture was kept at room temperature for ten minutes before a solution of

adenosine 5'-monophosphate (10.3 mg, 0.03 mmole) in D O/pyridine (10:1, 110 μl) was

added. The reaction was monitored by ³¹P-NMR and quenched with water (2 ml) after 3 hours. The mixture was extracted with EtOAc (2 x 3 ml). The aqueous layer was lyophilized. The resulting red solid was dissolved in a mixture of DMSO/H₂O (1:1, 2 ml),

filtered and purified on a 150 x 21.2 mm Polaris 5 μ C18-A column (MetaChem) at a flow

rate of 20 ml/min with a gradient of 0.1% TFA / 2% CH₃CN / H₂O to 0.1% TFA/100% CH₃CN H₂O over 30 min. The fractions were monitored at 550 nm. The fractions containing the product (RT=15.5 min) were pooled and lyophilized to give the title

compound 6 as a white solid (7.3 mg, 45%): 1H-NMR (400MHz, D₂O) δ 8.63 (s, IH), 8.46

(s, IH), 6.19 (d, J = 5.6 Hz, IH, H-1'), 4.75 (m, IH), 4.52 (m, 2H), 4.39 (m, IH), 4.34 (m, IH), 4.24 (m, IH), 3.20 (m, IH), 2.90 (dd, IH), 2.68 (d, IH), 2.36 (t, J = 7.0 Hz, 2H), 1.55

(m, 3H), 1.40 (m, IH), 1.30 (m, 2H); ³¹P-NMR (162 MHz, D₂O) δ -6.37 (s, IP); LRMS (ESI, [M + H]⁺) calculated for C₂₀H₂₉N₇O₉PS: 574; found: 574.

[00131] Example 7: Azide-PEG-Acyl-AMP (7)

(7) [00132] This compound was prepared using the procedure described for compound 6.

1H-NMR (400MHz, D₂O) δ 8.56 (s, IH), 8.40 (s, IH), 6.16 (d, J = 5.2 Hz, IH, H-1'), 4.75

(m, IH), 4.50 (m, IH), 4.38 (m, IH), 4.28 (s, 2H), 4.25 (m, 2H), 4.08 (s, 2H), 3.68 (m,

30H), 3.61 (m, 2H), 3.48 (m, 2H), 3.42 (m, 2H); ³¹P-NMR (162 MHz, D₂O) δ -6.69 (s, IP);

LRMS (ESI, [M + H]⁺) calculated for C₃₂H₅₅N₉Oι₈P: 884; found: 884. [00133] Example 8: (+)-Biotin-Hex-Acyl-AMP (8)

(8)

[00134] This compound was prepared using the procedure described for compound 6.

1H-NMR (400MHz, D₂O) δ 8.49 (s, IH), 8.33 (s, IH), 6.06 (d, J = 5.6 Hz, IH, H-1'), 4.63

(m, IH), 4.42 (m, IH), 4.39 (m, IH), 4.27 (m, 2H), 4.11 (m, 2H), 3.15 (m, IH), 3.00 (m, 2H), 2.95 (dd, IH), 2.63 (d, IH), 2.27 (t, J = 7.0 Hz, 2H), 2.09 (t, J = 7.0 Hz, 2H), 1.43 (m,

8H), 1.20 (m, 4H); ³¹P-NMR (162 MHz, D₂0) δ -6.42 (s, IP); LRMS (ESI, [M + H]⁺)

calculated for C₂₆H₄oN₈Oι₀PS: 687; found: 687.

[00135] Example 9: Fmoc-L-Lys(ε-(+)-Biotin)-Acyl-AMP (9):

(9) [00136] This compound was prepared using the procedure described for compound 6.

1H-NMR (400MHz, DMSO-d₆) δ 8.34 (s, 0.7H), 8.22 (s, 0.3H), 8.07 (s, 0.7H), 7.95 (s, 0.3

H), 7.63 (d, 1.4H), 7.55 (t, 0.6 H), 7.41-7.14 (m, 6H), 5.80 (d, J = 5.2 Hz, IH), 4.50-3.60 ( , 11H), 2.98 (m, 3H), 2.67 (dd, IH), 2.50 (m, IH), 2.01 (m, IH), 1.44-1.13 (m, 12 H);

³¹P-NMR (162 MHz, DMSO-d₆) δ -6.90 (s, 0.8P), -7.37 (s, 0.2P); LRMS (ESI, [M + H]⁺)

calculated for C₄ιH₅ιN₉O₂₂PS: 924; found: 924. [00137] Example 10: Azide-PEG-C3-Acyl-AMP (10):

(10)

[00138] This compound was prepared using the procedure described for compound 6.

1H-NMR (400MHz, D₂O) δ 8.60 (s, IH), 8.44 (s, IH), 6.19 (d, J = 4.8 Hz, IH, H-1'), 4.75

(m, IH), 4.50 (m, IH), 4.39 (m, IH), 4.23 (m, 2H), 3.70 (m, 10H), 3.59 (m, 2H), 3.47 (m, 2H), 3.36 (m, 2H), 2.44 (t, J = 7.4 Hz, 2H), 2.25 (t, J = 7.6 Hz, 2H), 1.82 (m, 2H); ³¹P-NMR

(162 MHz, D₂O) δ -6.47 (s, IP); LRMS (ESI, [M + H]⁺) calculated for C₂₃H₃₇N₉Oι₂P: 662;

found: 662. [00139] Example 11: (+)-Biotin-Hex-PEG4-Acyl-AMP (11)

(11) [00140] This compound was prepared using the procedure described for compound 6.

1H NMR (400MHz, D₂O) δ 8.48 (s, IH), 8.30 (s, IH), 6.06 (d, J = 5.2 Hz, IH, H-1'), 4.64

(m, IH), 4.42 (m, IH), 4.39 (m, IH), 4.27 (m, 2H), 4.11 (m, 2H), 3.56 (m, 16H), 3.25 (m, 2H), 3.20 (m, IH), 2.55 (dd, IH), 2.63 (m, 3H), 2.12 (t, J = 7.4 Hz, 2H), 1.50 (m, 4H), 1.25

(m, 2H); ³¹P NMR (162 MHz, D₂O) δ -6.59 (s, IP); LRMS (ESI, [M + H]⁺) calculated for

C₃ιH₅₀N₈O₁₄PS: 821; found: 821. [00141] Example 12: (-t-)-Biotin-Acyl-ATP (12)

o a st rre suspens on o + - ot n . mg, . mmo e n a m xture o

solvents (dioxane/DMF/DMSO, 1:1:1, 3 ml) was added triethylamine (19.9 μl, 0.14 mmole)

and isobutyl chloroformate (12.3 μl, 0.10 mmole) at 0°C. The mixture was kept at that

temperature for 5 minutes and was allowed to warm up to room temperature and stirred for 1.5 hours. A solution of ATP bistriethylammonium salt (32.8 mg, 0.05 mmole) in DMSO (1 ml) was added to the above mixture to give a clear solution. The reaction was monitored

by P-NMR by preparing a sample of 500 μl of the reaction mixture and 100 μl of D₂O (or

DMSO-d₆). After 20 hours 1 ml of the solution was drawn from the reaction mixture and water (2 ml) was added. The solution was extracted with ethyl acetate (2 x 3 ml). The aqueous layer was lyophilized. The resulting solid was suspended in water (1 ml) and purified by a short C18 column (14 x 45 mm) using a gradient of water to 40% acetonitrile/water to give the title compound 12 as a white powder: 1H-NMR (400MHz, D₂O) δ 8.57 (s, IH), 8.22 (s, IH), 6.13 (d, J = 6 Hz, IH, H-1'), 4.75 (m, IH), 4.55 (m, 2H),

4.39 (m, IH), 4.30 (m, IH), 4.24 (m, 2H), 3.19 (q, J = 7.2 Hz, 12H), 3.15 (m, IH), 2.90 (dd, IH), 2.70 (m, IH), 2.36 (t, J = 7.4 Hz, 2H), 1.47 (m, 4H), 1.26 (t, J = 7.2 H, 18H), 1.21 (m,

2H); ³¹P-NMR (162 MHz, D₂O) δ -10.41 (d, J = 19. 6 Hz, IP), -18.70 (d, J = 19.9 Hz, IP), -

22.64 (t, J = 19.8 Hz, IP); LRMS (ESI, [M - H]^") calculated for C₂₀H₂₉N₇Oι₅P₃S: 732; found: 732.

[00143] Example 13: (+)-Biotin-Hex-Acyl-ATP (13)

(13)

[00144] This compound was prepared using the procedure described for compound

12. 1H-NMR (400MHz, D₂O) δ 8.57 (s, IH), 8.28 (s, IH), 6.12 (d, J = 6.0 Hz, IH, H-1'),

4.75 (m, IH), 4.56 (m, 2H), 4.36 (m, 2H), 4.22 (m, 2H), 3.24 (m, IH), 3.19 (q, J = 7.2 Hz, 12H), 3.09 (m, 2H), 2.95 (dd, IH), 2.74 (d, IH), 2.37 (m, 2H), 2.20 (t, J = 7.0 Hz, 2H), 1.50

(m, 6H), 1.38 (m, 6H), 1.26 (t, J = 7.2 H, 18H); ³¹P-NMR (162 MHz, D₂O) δ -10.44 (d, J =

19.8 Hz, IP), -18.71 (d, J = 19.6 Hz, IP), -22.66 (t, J = 19.4 Hz, IP). [00145] Example 14: Azide-PEG-C3-Acyl-ATP (14)

(14)

[00146] This compound was prepared using the procedure described for compound

12. . 1H-NMR (400MHz, D₂O) δ 8.51 (s, IH), 8.27 (s, IH), 6.02 (d, J = 5.6 Hz, IH, H-1'),

4.63 (m, IH), 4.44 (m, IH), 4.29 (m, IH), 4.14 (m, 2H), 3.59 (m, 10H), 3.48 (t, J = 5.4 Hz, 2H), 3.36 (m, 2H), 3.23 (t, J = 5.4 Hz, 2H), 3.06 (q, J = 7.3 Hz, 12H), 2.35 (t, J = 7.2 Hz, 2H), 2.15 (t, J = 7.8 Hz, 2H), 1.73 (m, 2H), 1.14 (t, J = 7.4 Hz, 18H); ³¹P-NMR (162 MHz,

D₂O) δ -10.45 (d, J = 19.' 1 Hz, IP), -18.81 (d, J = 19.8 Hz, IP), -22.66 (t, J = 19.6 Hz, IP);

LRMS (ESI, [M - H]^") calculated for C₂₃H₃₇N₉Oι₈P₃: 820; found: 820. [00147] Example 15: (+)-Biotin-Hex-PEG4-Acyl-ATP (15)

(15)

[00148] This compound was prepared using the procedure described for compound

12. 1H-NMR (400MHz, D₂O) δ 8.56 (s, IH), 8.28 (s, IH), 6.13 (d, J = 6.4 Hz, IH, H-1'),

4.75 (m, IH), 4.56 (m, 2H), 4.39 (m, 2H), 4.24 (m, 2H), 3.66 (m, 16H), 3.37 (m, 2H), 3.30 (m, IH), 3.20 (t, J = 7.3 Hz, 12H), 2.95 (dd, IH), 2.73 (m, 3H), 2.24 (t, J = 7.4 Hz, 2H),

1.65 (m, 4H), 1.34 (m, 2H), 1.26 (t, J = 7.4 Hz, 18H); ³¹P-NMR (162 MHz, D₂0) δ -10.45

(d, J = 19. 1 Hz, IP), -18.81 (d, J = 19.6 Hz, IP), -22.67 (t, J = 19.6 Hz, IP); LRMS (ESI, [M - HD calculated for C₃ιH₅₀N₈O₂₀P₃S: 979; found: 979. [00149] Example 16: (+)-Biotin-Acyl-ADP (16):

(16)

[00150] This compound was prepared using the procedure described for compound

12. 1H-NMR (400MHz, D₂O) δ 8.55 (s, IH), 8.28 (s, IH), 6.14 (d, J = 6 Hz, IH, H-1'), 4.74

(m, IH), 4.51 (m, 2H), 4.39 (m, IH), 4.27 (m, IH), 4.24 (m, 2H), 3.19 (q, J = 7.2 Hz, 12H), 3.15 (m, IH), 2.90 (dd, IH), 2.70 (m, IH), 2.31 (t, J - 7.4 Hz), 1.47 (m, 4H), 1.27 (t, J = 7.2

H, 18H), 1.16 (m, 2H); ³¹P-NMR (162 MHz, D₂O) δ -10.72 (d, J = 22.5 Hz, IP), -18.75 (d,

J = 21.5 Hz, IP); LRMS (ESI, [M - H]^") calculated for C₂oH₂₉N₇Oi₂P₂S: 652; found: 652. [00151] Example 17: Azide-PEG-C3-Acyl-ADP (17)

(17)

[00152] This compound was prepared using the procedure described for compound

12. 1H-NMR (400MHz, D₂0) δ 8.51 (s, IH), 8.26 (s, IH), 6.13 (d, J = 6.0 Hz, IH, H-1'),

4.75 (m, IH), 4.52 (m, IH), 4.38 (m, IH), 4.21 (m, 2H), 3.67 (m, 10H), 3.56 (t, J = 5.4 Hz, 2H), 3.47 (m, 2H), 3.31 (t, J = 5.4 Hz, 2H), 3.19 (q, J = 7.3 Hz, 12H), 2.40 (t, J = 7.2 Hz, 2H), 2.20 (t, J = 7.8 Hz, 2H), 1.80 (m, 2H), 1.27 (t, J = 7.4 Hz, 18H); ³¹P-NMR (162 MHz, D₂O) δ -10.70 (d, J = 21.7 Hz, IP), -18.73 (d, J = 21.7 Hz, IP). [00153] Example 18: (+)-Biotin-Hex-Acyl-ADP (18)

(18)

[00154] This compound was prepared using the procedure described for compound

12. 1H-NMR (400MHz, D₂O) δ 8.54 (s, IH), 8.28 (s, IH), 6.14 (d, J = 6.0 Hz, IH, H-1'),

4.75 (m, IH), 4.52 (m, 2H), 4.37 (m, 2H), 4.22 (m, 2H), 3.22 (m, IH), 3.17 (q, J = 7.2 Hz, 8H), 3.05 (m, 2H), 2.95 (dd, IH), 2.74 (d, IH), 2.37 (t, J = 7.2 Hz, 2H), 2.20 (t, J = 7.0 Hz, 2H), 1.50 (m, 4H), 1.45 (m, 2H), 1.32 (m, 2H), 1.26 (t, J = 7.2 H, 12H), 1.17(m, 2H); ³¹P-

NMR (162 MHz, D₂O) δ -10.73 (d, J = 22.0 Hz, IP), -18.73 (d, J = 21.9 Hz, IP); LRMS

(MALDI, [M + H]⁺) calculated for C₂₆H₄ιN₈Oι₃P₂S: 767; found: 767. [00155] EXAMPLE 19: (+)-Biotin-Hex-PEG4-Aeyl-ADP (19)

(19)

[00156] This compound was prepared using the procedure described for compound

12. 1H NMR (400MHz, D₂O) δ 8.54 (s, IH), 8.29 (s, IH), 6.14 (d, J = 6.0 Hz, IH, H-1'),

4.75 (m, IH), 4.56 (m, 2H), 4.39 (m, 2H), 4.22 (m, 2H), 3.66 (m, 16H), 3.37 (m, 2H), 3.30 (m, IH), 3.20 (t, J = 7.3 Hz, 12H), 2.95 (dd, IH), 2.75 (m, IH), 2.73 (t, 3H), 2.23 (t, J = 7.4 Hz, 2H), 1.65 (m, 4H), 1.36 (m, 2H), 1.27 (t, J = 7.4 Hz, 18H); ³¹P NMR (162 MHz, D₂O) δ

-10.67 (d, J = 21.5 Hz, IP), -18.87 (d, J = 21.7 Hz, IP).

[00157] Example 20: (+)-Biotin-Hex-Acyl-AMPCP (20)

(20)

[00158] This compound was prepared using the procedure described for compound

12. 1H-NMR (400MHz, D₂0) δ 8.69 (s, IH), 8.40 (s, IH), 6.14 (d, J = 5.2 Hz, IH, H-1'),

4.75 (m, IH), 4.58 (m, IH), 4.54 (m, IH), 4.40 (m, 2H), 4.22 (m, 2H), 3.28 (m, IH), 3.17 (q, J = 7.2 Hz, 6H), 3.11 (m, 2H), 2.95 (dd, IH), 2.74 (d, IH), 2.43 (t, J = 20.0 Hz), 2.37 (t, J = 7.2 Hz, 2H), 2.20 (t, J = 7.0 Hz, 2H), 1.55 (m, 6H), 1.43 (m, 2H), 1.32 (m, 2H), 1.26 (t, J

= 7.2 H, 12H); ³¹P-NMR (162 MHz, D₂0) δ 17.22 (d, J = 11.2 Hz, IP), 14.71 (d, J = 11.2

Hz, IP); LRMS (ESI, [M - H]^") calculated for C₂₇H₄ιN₈Oi₂P₂S: 763; found: 763.

[00159] Example 21: (+)-Biotin-Pent-Acyl-ADP (21)

(21) [00160] This compound was prepared using the procedure described for compound

12. 1H-NMR (400MHz, D₂O) δ 8.65 (s, IH), 8.43 (s, IH), 6.18 (d, J = 5.6 Hz, IH, H-1'),

4.75 (m, IH), 4.56 (m, IH), 4.54 (m, IH), 4.39 (m, 2H), 4.23 (m, 2H), 3.22 (m, IH), 3.17 (q, J = 7.2 Hz, 4H), 3.12 (m, 2H), 2.95 (dd, IH), 2.74 (d, IH), 2.37 (t, J = 7.2 Hz, 2H), 2.21 (t, J = 7.0 Hz, 2H), 1.59 (m, 8H), 1.35 (m, 2H), 1.27 (t, J = 7.2 H, 4H); ³¹P-NMR (162 MHz,

D₂0) δ -10.70 (d, J = 21.7 Hz, IP), -18.64 (d, J = 21.5 Hz, IP); LRMS (ESI, [M - H]^")

calculated for C₂₅H₃₇N₈Oι₃P₂S: 751; found: 751.

[00161] Example 22: (+)-Biotin-Pen-Acyl-ATP (22)

(22)

[00162] This compound was prepared using the procedure described for compound

12. 1H-NMR (400MHz, D₂O) δ 8.59 (s, IH), 8.32 (s, IH), 6.12 (d, J = 6.0 Hz, IH, H-1'),

4.75 (m, IH), 4.55 (m, 2H), 4.38 (m, 2H), 4.25 (m, 2H), 3.20 (m, IH), 3.17 (q, J = 7.2 Hz, 12H), 3.09 (m, 2H), 2.95 (dd, IH), 2.73 (d, IH), 2.43 (m, 2H), 2.20 (t, J = 7.0 Hz, 2H), 1.55 (m, 10H), 1.26 (t, J = 7.2 H, 18H); ³¹P-NMR (162 MHz, D₂O) δ -10.68 (d, J = 19. 2 Hz,

IP), -18.75 (d, J = 19.4 Hz, IP), -22.62 (t, J = 19.6 Hz, IP); LRMS (MALDI, [M + H]⁺) calculated for C₂₅H₄₀N₈Oι₆P₃S: 833; found: 833. [00163] Example 23: Alkyne-Acyl-ADP (23)

[00164] This compound was prepared according to the procedure for compound 12.

1H-NMR (400MHz, D₂O) δ 8.53 (s, IH), 8.27 (s, IH), 6.13 (d, J = 6.0 Hz, IH, H-1'), 4.75

(m, IH), 4.51 (m, IH), 4.38 (m, IH), 4.22 (m, 2H), 3.19 (q, J - 7.2 Hz, 12H), 2.32 (t, J = 7.4

Hz, 2H), 2.24 (s, IH), 2.05 (t, J = 7.0 Hz, 2H), 1.49 (m, 2H), 1.32 (m, 2H), 1.26 (t, J = 7.2

H, 18H); ³¹P-NMR (162 MHz, D₂O) δ -10.73 (d, J = 22.1 Hz, IP), -18.74 (d, J = 22.1 Hz,

IP).

[00165] Example 24: TAMRA-5' -Triazole- Aeyl-ADP (24)

[00166] A solution of TAMRA-5'-CONH-(CH₂O)₃-CH2CH2-N3 (4.0 mg, 6.3

μmole), compound 23 (4.7 mg, 6.3 μmole), sodium ascorbate (0.6 mg, 3.2 μmole) and

copper sulfate pentahydrate (0.4 mg, 1.6 μmole) in 2 mL of water was kept at 37°C for two hours and was then lyophilized. The residue was dissolved in water and purified by a short C18 column (14 x 45 mm) using a gradient of water to 80% acetonitrile/water to give the

title compound 24 as a red powder: ³¹P-NMR (162 MHz, D₂O) δ -10.78 (m, IP), -18.70 (m,

IP); LRMS (MALDI, [M - H]^") calculated for C₅oH₆ιNπOι₈P₂: 1165; found: 1165.

[00167] Example 25: TAMRA-6'-Carbamate-Triazole-Acyl-ADP (25)

[00168] This compound was prepared according to the procedure for compound 24:

LRMS (MALDI, [M - H]^") calculated for C₄₈H₅₅Nι₂Oι₇P₂: 1133; found: 1133.

[00169] Example 26: TAMRA-6'-Reversed Carbamate-Triazole-Acyl-ADP (26)

[00170] This compound was prepared according to the procedure for compound 24:

LRMS (MALDI, [M - HD calculated for C₄₈H₅₅Nι₂Oι₇P₂: 1133; found: 1133.

[00171] Example 27: Alkyne-Acyl-ATP (27)

[00172] This compound was prepared according to the procedure described for

compound 12. 1H-NMR (400MHz, D₂O) δ 8.53 (s, IH), 8.29 (s, IH), 6.02 (d, J = 5.6 Hz,

IH, H-1'), 4.63 (m, IH), 4.44 (m, IH), 4.29 (m, IH), 4.16 (m, 2H), 3.08 (q, J = 7.2 Hz, 12H), 2.34 (t, J = 7.2 Hz, 2H), 2.16 (s, IH), 2.03 (t, J = 7.2 Hz, 2H), 1.51 (m, 2H), 1.34 (m,

2H), 1.15 (t, J = 7.2 H, 18H); ³¹P-NMR (162 MHz, D₂O) δ -10.45 (d, J = 19.6 Hz, IP), - 18.69 (d, J = 19.8 Hz, IP), -22.56 (d, J = 19.4 Hz, IP).

[00173] Example 28: TAMRA-5'-PEG-Triazole-Acyl-ATP (28)

[00174] This compound was prepared according to the procedure described for compound 24: LRMS (MALDI, [M - H]^") calculated for C₅₀H₆₂Nιιθ2iP3: 1244; found: 1244.

[00175] Example 29: Biotin-Acyl-CTP (29)

(29) [00176] This compound was prepared according to the procedure described for compound 12. ^!H NMR (400MHz, D₂0) δ 8.1 (d, 2H), 6.2 (d, IH), 5.8 (d, IH), 4.1-4.3 (m,

6H), 3.9 (d, 2H), 3.1 (q, 15H), 1.2 (t, 27H), 0.8 (d, 7H). ³¹P-NMR (162 MHz, D₂O) δ-10.38

(d, J = 19.4 Hz, IP), -19.17 (d, J = 18.0, IP), -22.8 (t, J = 17.8 Hz, IP).

[00177] Example 30: Biotin-Acyl-GTP (30)

(30) [00178] This compound was prepared according to the procedure described for compound 12. 1H NMR (400MHz, D₂O) δ 8.0 (s, IH), 5.9 (d, IH), 4.5 (m, 2H), 4.2 (m, 2H), 4.1 (m, 2H), 3.1 (q, 20H), 2.9 (d, IH), 2.6 (d, IH). 1.1 (t, 34H), 1.0 (d, 3H). ³¹P-NMR (162 MHz, D₂O) δ -10.5 (d, J = 26.2, IP), -19.0 (d, J = 19.76, IP), -22.7 (t, J = 19.1, IP). MALDI, [M - H]^~ calculated for C₂₆H₄ιN₈Oι₇P₃S: 862.63; found: 861.3 (M-H)

[00179] Example 31: Biotin-Acyl-GDP (31)

[00180] This compound was prepared according to the procedure described for compound 12. 1H NMR (400MHz, D₂O) δ 7.9 (s, IH), 5.8(d, IH), 4.5(t, IH), 4.4 (t, IH), 4.3 (m, 2H), 4.1 (m, 2H), 3.1 (q, 14H), 2.9 (q, IH), 2.8 (m, IH), 2.6 (m, IH), 2.2 (t, 2H), 2.1 (t, 2H), 1.3-1.5 (m, 7H), 1.1 (t, 27H), 1.0 (d, IH). ³¹P-NMR (162 MHz, D₂O) δ -10.7 (d, J = 21.2, IP), -18.7 (d, J = 22.0, IP).

[00181] Example 32: Biotin-Acyl-UTP (32)

(32)

[00182] ^* This compound was prepared according to the procedure described for compound 12. 1H NMR (400 MHz, D O) δ 7.9 (d, IH), 5.8(d, IH), 4.6 (m, 4H), 4.3 (m, 4H), 4.1-4.2 (d, 4H), 3.1 (m, 27H), 2.8 (m, 2H), 2.68 (d, IH), 2.4 (t, 2H), 2.1 (t, 2H), 2.4-2.6 (m, 10H), 1.2 (t, 39H), 1.0 (d, 2H). ³¹P-NMR (162 MHz, D₂O) δ -10.6 (d, J = 18.1, IP), - 18.7 (d, J = 19.4, IP), -22.7 (t, J = 19.6, IP).

[00183] Example 33: Biotin- Acyl-UDP (33)

[00184] This compound was prepared according to the procedure described for compound 12. 1H NMR (400 MHz, D₂0) δ 7.9 (d, IH), 5.9(m, IH), 4.6 (m, 4H), 4.1-4.3 (m, 9H), 3.2 (m, 3H), 3.0 (m, 17H), 2.7-2.8 (m, 2H), 2.6-2.7 (m, 2H), 2.3 (t, 3H), 2.11 (t, 3H), 1.3-1.5 (m, 14H), 1.16 (t, 29H). ³¹P-NMR (162 MHz, D₂O) δ -10.7 (d, J = 21.2, IP), - 18.7 (d, J = 21.4, IP). [00185] Example 34: Biotin- Acyl-CDP (34)

34

[00186] This compound was prepared according to the procedure described for compound 12. 1H NMR (400MHz, D₂O) δ 7.9 (d, IH), 6.1 (d, IH), 5.8(d, IH), 4.5 (m, 3H), 4.1-4.3 (m, 8H), 3.1 (q, 18H), 2.9 (m, IH), 2.7 (m, IH), 2.3-2.4 (m, 3H), 2.2 (t, 3H), 1.3-1.5 (m, 10H), 1.2 (25H), 0.9 (2H). ³¹P-NMR (162 MHz, D₂O) δ -10.7 (d, J = 21.87 Hz, IP), -18.6 (d, J = 21.7 Hz, IP).

[00187] Example 35: Labeling of polypeptides

[00188] The following is a procedure for preparing and analyzing samples from primary tissue according to methods of the present invention. Exemplary components needed are a mortar and pestle, cryule vials, labels, Eppendorf 1.5 ml tubes, Beckman tubes for TL100.3 rotor, TL100 ultracentrifuge, spatulas, liquid nitrogen in dewar, dry ice, Omni 5 mm probe and homogenizer, sonicator, beakers for waste and washes, pipettors, 50 mM tris buffer pH 7.4 +/- 0.1 % Triton XI 00, BioRad Dc protein assay, microtiter plate and reader, 2x SDS-PAGE loading buffer, FP probe.

[00189] Flash-frozen tissue is crushed into ~1 mm pieces or smaller in pool of liquid nitrogen using a ceramic pestle and mortar. With the help of a spatula, frozen pieces are transferred into a crayule vial on dry ice. The liquid nitrogen is allowed to vaporize before capping. About 0.1 g of tissue is then transfened into an Eppendorf tube for processing, keeping all samples on dry ice. The 0.1 g of frozen tissue is fransfened from the Eppendorf tube to a 12x75 mm polypropylene round bottom tube. Approximately 400 μl of cold 50 mM Tris, pH 7.4, is added to each sample. Each sample is then homogenized with a 5 mm stainless steel Omni probe using 2 x 4 sec bursts at highest speed, making sure to keep the tube on ice the entire time.

[00190] In between samples, the homogenizer probe tip is washed by running it in a large beaker of water, replacing this water often and bleaching the waste. Any fibers are removed out of the probe tip with tweezers, and the end of the probe is blotted with a Kimwipe to remove trapped liquid.

[00191] The homogenized sample is sonicated using a microtip at setting 2.5, 4 x 3 second pulses, keeping the sample on ice the entire time. The sonicated sample is then fransfened a microcentrifuge tube and spun at 2000 x g for 10 min at 4 °C in a microcentrifuge to pellet unlysed material. The supernatant from this tube is then fransfened to Beckman tubes (# 357448) and spun in a prechilled ulfracentifuge at 64K φm (170,000 x g) at 4 °C for 1 hour. The supernatant (soluble protein fraction) is then fransfened to a fresh tube, leaving behind the membrane pellet (membrane bound protein fraction). The membrane pellet is rinsed with about 100 μl cold 50 mM Tris, pH 7.4, and solubilized with 400 μl cold 50 mM Tris pH 7.4 + 0.1% Triton X-100 buffer on ice using a sonicator.

[00192] The protein concentration of both soluble and membrane fractions is determined using the BioRad Dc protein assay (#500-0116) as follows. Serial dilutions of samples (neat, Y_, ^lA, V.) are tested using BSA standard concentrations of 1.4, 1.05, 0.787, 0.54, 0.44, 0.33, 0.249 and 0 mg/ml (³/ dilutions). Tris + 0.1% Triton buffer are used as the diluent and as the blank. In a 96 well microtiter plate, 5 μl of sample or standard is used per well, adding 25 μl Reagent A, then 200 μl Reagent B. The reaction color is developed for 15 minutes at room temperature and the plates read to determine the OD at 750 nm. Sample protein concentrations are then adjusted to 1 to 1.5 mg/ml with Tris or Tris/Triton buffer for soluble or membrane fractions, respectively.

[00193] A heated control sample is prepared by heating -60 μL of sample in a microcentrifuge tube in a block heater at 95 °C for 6 minutes prior to labeling. After heating, the sample is chilled down on ice, then spun in a microcentrifuge. Samples containing precipitate that does not disperse by vortexing may be sonicated prior to labeling.

[00194] Samples are labeled by adding probe to a lysate sample to a final concentration of 2 μM and mixed quickly by flicking the tube. A minimum volume of probe is used such that the amount of added probe did not exceed 5% of the final sample volume. Samples are typically labeled using 50 μl with 1 μl of 100 μM probe for lhour at room temperature. At the end of the labeling period, an equal volume (50 μl) of 2x SDS- PAGE loading buffer is added and the mixture heated at 95 °C for 6 minutes, cooled to room temperature, spun, and loaded on 12.5% SDS-PAGE gels. Long gels are loaded with 20 μg of samples and electrophoresed for 4 hours at 300 volts, and maximum current. The gels are then rinsed with water and wiped dry, keeping the gel in the glass plates for scaiining.

[00195] Example 36: Protein Identification

[00196] For identification of proteins by mass spectrometry, samples are prepared as described in the previous example through the probe labeling step. At the end of the labeling period, 80 mg urea is added per 100 uL of sample, and DTT is added to a final concentration of 10 mM from a fresh IM stock. The resulting mixture is heated to 65 °C for 20 minutes, then cooled to room temperature. Iodoacetamide is then added to a final concentration of 40 mM from a fresh IM stock. The resulting mixture is incubated at 37 °C for 45 minutes in the dark.

[00197] The sample as prepared above is then added to a desalting (Pharmacia PD10 or Bio-Rad 10DG) preequilabrated with 2M urea, 20 mM Ammonium Bicarbonate. The protein peak is identified by absorbance at 280 nm and collected.

[00198] 1/10 volume of 10% SDS is then added to the pooled protein fractions, and the mixture heated to 65 °C for 5 minutes. This is then diluted with 1 volume of 2X Binding

Buffer (2% Triton X-100, 1% Tergitol NP40 type, 300 mM NaCl, 2 mM EDTA, 20 mM

Tris pH 7.4). Antibody affinity beads (either monoclonal or goat polyclonal antibody directed to TAG are added using a cut off pipette tip (anywhere from 30-200 uL of 50% bead shiny to yield 15-100 uL of beads). The mixture is mixed by rocking at room temperature for from 2 hours to 15 hours.

[00199] The antibody beads are then pelleted by centrifugation, and the supernatant carefully removed and discarded. The beads are washed at least three times with 1 mL of binding buffer + 0.2% SDS. The beads are then washed twice with 0.5 mL of 50 mM tris,

100 mM NaCl to remove excess detergents.

[00200] Captured proteins are eluted with 1 bed volume of IX non-reducing loading/elution buffer (50 mM Tris pH 7.5, 10% glycerol, 5% SDS, 150 mM NaCl, bromophenol blue (5 mg/50mL)). The beads are allowed to sit in this buffer at 65 °C for 10 minutes when monoclonal antibodies are employed for capture. For goat polyclonal antibody beads, captured proteins are eluted at room temperature for 10 minutes. The sample (beads and buffer liquid) are then loaded onto a micro spin column and spun at 5000 m for 3 minutes in a microcentrifuge for collection of eluted proteins.

[00201] If goat polyclonal antibodies are used for capture, the eluted proteins are loaded directly onto an SDS-PAGE gel. If monoclonal antibodies are used, DTT is added to 10 mM, and the resulting solution is boiled briefly before loading onto the gel. Following electrophoresis and staining, sections of the gel containing the protein bands of interest are excised, the gel pieces cut into several small pieces and destained with methanol, washed with 100 mM ammonium bicarbonate in 30% acetonitrile a few times, and the proteins digested with trypsin (100 ng) in 3 mM Tris-HCl at pH 8, at 37 °C overnight. The tryptic peptides are extracted out of the gel using 50%> acetonitrile/ 0.1% TFA, concentrated to 10 μl, and subjected to nano-capillpary HPLC-tandem mass spectrometry (MS/MS) for analysis. This analysis is performed on a combination system of Agilent 1100 capillary HPLC/Micro Auto-sampler (Agilent Technologies, Palo Alto, CA) and Finnigan LCQ DecaXP ion trap mass spectrometer (Finnigan, San Jose, CA).

[00202] Liquid chromatographic separation is performed on 3 μl of digested sample mixed with 3 μl of 5% acetic acid, loaded onto a 100 μm fused silica capillary C₁₈ column. A sixty minute gradient of 5-95% solvent B (A: H₂θ/0.1% formic acid, B: MeCN/0.08 % formic acid) and a 500 nl/minute column flow rate is used to separate the tryptic peptides in the digested sample. Peptides eluted off the column are directly injected into LCQ DecaXP mass spectrometer.

[00203] The heated desolvation capillary in mass spectrometer is held at 200 °C, the spray voltage is set at 2.0 kV, and the capillary voltage is set at 30 V. During the experiment, the mass spectrometer is set to alternate between MS and MS/MS mode. The scan range for MS was set at m/z 400-1600. The MS/MS spectra are acquired in dependent

scan mode with an initiating minimum MS signal at 2x10 counts, and a 35% normalized collision energy. The scan range for MS/MS is varied from 80-2000 depending on the precursor ion.

[00204] The ion masses and the fragmentation information generated by nano-

LCMS/MS experiment are analyzed and converted into peptide masses and sequence information with TurboSEQUEST, which is protein identification software. Using this program, peptide sequence information may be compared against the protein database to identify proteins. [00205] EXAMPLE 37: Labeling of polypeptides

[00206] For tissue culture cells, media is aspirated and cells rinsed twice with 10 ml

PBS, adding the PBS onto the side of the dish. Cells are harvested by scraping into in extraction buffer (50mM Tris, pH 7.5, ImM EDTA, 0.5mM EGTA, 5ug/ml each of protease inhibitors Aprotinin, Pepstatin, Leupeptin, lOOmM PMSF) and then fransfened to a 1ml glass douncer. Cells are dounced up and down 20 times on ice. Then cell lysates are sonicated using a microtip at setting 2.5, using 4 sec pulses, 3 times. Samples are kept on ice during the procedure. After the sample is spun in microcentrifuge tube at 1.0 K φm for 10 min at 4C in the microcentrifuge to pellet unlysed material it is spun at 100-110,000 x g for Hi at 4C. The supernatent (cytosol) is collected and the membrane pellet washed by brief sonication in tris buffer followed by centrifugation. The washed membrane pellet is then solubilized in extraction buffer containing 0.1% Triton X-100 detergent and sonicated again. The protein concentration of both cytosol and membrane fractions is determined using the BioRad Dc protein assay. Serial dilutions of samples (neat, Vi, ^lA, 1/8) and BSA standard concentrations of 1.4, 1.05, 0.787, 0.54, 0.44, 0.33, 0.249 and 0 mg/ml (3/4 dilutions) are tested using Tris buffer as the diluent and as the blank. Sample protein concentrations are adjusted to 5 mg/ml with extraction buffer. The acylphosphate probe is

then added to 5mg of extract in a volume of 1ml at a final concentration of lOμM and mixed

by flicking the tube. Labeling occurs for lh at RT. After labeling is completed 800 mg of urea and DTT to lOmM final concentration from a fresh IM stock is added. The sample is heated to 65 °C for 15 min. [00207] After cooling to room temperature Iodoacetamide is added to 40 mM from a fresh IM stock and the sample incubated at 37 °C for 30 minutes in the dark. After equilibration of a Bio-Rad 10 DG gel filtration column with 2M urea, 10 mM Ammonium Bicarbonate, 5 mM methionine the labeled protein sample is applied to column and fractions collected. The absorbance at A₂₈₀ is followed to find and collect the protein peak.

10 μL of 20%) triton X-100 and 30 μL sequencing grade modified trypsin (Promega) is

added to the purified sample and the digest incubated at 37 °C for lh. Following the digest

of the sample 100 μL of 10%> SDS is added to the digested sample and heated to 65 °C for 5 minutes. The protein sample is then diluted with 1 volume of 2X Binding Buffer (2%» Triton

X-100, 1% Tergitol NP40 type, 300 mM NaCl, 2 mM EDTA, 20 mM Tris pH 7.4). 100 μL

of a 50%» s iny of avidin-beads (Upstate Biotechnology) are added and the sample rocked at room temperature for 1 h. The beads are then spun down and the supernatant removed by aspiration. The beads are then fransfened to a microspin column that is set on a 2 mL eppendorf tube. The column is spun briefly in a nanofuge for 3-5 seconds to drain the liquid. The beads are then washed 2X more with 1 mL of IX binding buffer +1% SDS. [00208] Beads are then washed 3X with 1 mL of IX PBS and then 3X with 1 mL of

ddH20. Captured peptides are then eluted with 2 separate 50 μL volumes of freshly

prepared 50%> Acetonitrile with 0.1 % TFA and the eluates analyzed by mass spectrometry. [00209] EXAMPLE 38: Identification of labeled proteins

[00210] Using the methods of the present invention, the following table lists proteins that have been identified by labeling with nucleotide-based TAPPs:

Protein kinases

AAK1_HUMAN 5'-AMP-activated protein kinase, catalytic alpha-1 chain (EC 2.7.1.-) (AMPK alpha-1 chain). [Homo sapiens] AAK1_RAT 5'-AMP-activated protein kinase, catalytic alpha-1 chain (EC 2.7.1.-) (AMPK alpha-1 chain). [Rattus norveglcus] AAK2_HUMAN 5'-AMP-activated protein kinase, catalytic alpha-2 chain (EC 2.7.1.-) (AMPK alpha-2 chain). [Homo sapiens] AAKG_HUMAN 5'-AMP-activated protein kinase, gamma-1 subunit (AMPK gamma-1 chain) (AMPKg). [Homo sapiens] ABL1_HUMAN Proto-oncogene tyrosine-proteln kinase AB 1 (EC 2.7.1.112) (pl50) (c-ABL). [Homo sapiens] ABL2_HUMAN Tyrosine-protein kinase ABL2 (EC 2.7.1.112) (Tyrosine kinase ARG). [Homo sapiens] AKT2_HUMAN RAC-beta serine/threonine protein kinase (EC 2.7.1.-) (RAC-PK-beta) (Protein kinase Akt-2) (Protein kinase

B, beta) (PKB beta). [Homo sapiens] ANR3JHUMAN Serine/threonine-protein kinase ANKRD3 (EC 2.7.1.-) (Ankyrin repeat domain protein 3) (PKC-delta- interacting protein kinase). [Homo sapiens]

ARK1_HUMAN Beta-adrenergic receptor kinase 1 (EC 2.7.1.126) (Beta-ARK-1) (G- protein coupled receptor kinase 2).

[Homo sapiens]

ARK1_RAT Beta-adrenergic receptor kinase 1 (EC 2.7.1.126) (Beta-ARK-1) (G- protein coupled receptor kinase 2).

[Rattus norvegicus]

ARK2_HUMAN Beta-adrenergic receptor kinase 2 (EC 2.7.1.126) (Beta-ARK-2) (G-proteln coupled receptor kinase 3).

[Homo sapiens]

BCKD_HUMAN [3-methyl-2-oxobutanoate dehydrogenase [lipoamlde]] kinase, mitochondrial precursor (EC 2.7.1.115)

(Branched-chain alpha-ketoacid dehydrogenase kinase) (BCKDHKIN) (BCKD-klnase). [Homo sapiens]

BCR_HUMAN Breakpoint cluster region protein (EC 2.7.1.-). [Homo sapiens] BTK_HUMAN Tyrosine-proteln kinase BTK (EC 2.7.1.112) (Bruton's tyrosine ki CDC2_HUMAN Cell division control protein 2 homolog (EC 2.7.1.-) (p34 protein kinase) (Cyclin-dependent kinase 1)

(CDK1). [Homo sapiens]

CDC2_MOUSE Cell division control protein 2 homolog (EC 2.7.1.-) (p34 protein kinase) (Cyclin-dependent kinase 1)

(CDK1). [Mus musculus]

CDC2_RAT Cell division control protein 2 homolog (EC 2.7.1.-) (p34 protein kinase) (Cyclin-dependent kinase 1)

(CDK1). [Rattus norvegicus]

CDK2_HUMAN Cell division protein kinase 2 (EC 2.7.1.-) (p33 protein kinase). [Homo sapiens] CDK2_M0UΞE Cell division protein kinase 2 (EC 2.7.1.-). [Mus musculus] CDK2_RAT Cell division protein kinase 2 (EC 2.7.1.-). [Rattus norvegicus] CDK5_HUMAN Cell division protein kinase 5 (EC 2.7.1.-) (Tau protein kinase II catalytic subunit) (TPKII catalytic subunit)

(Serine/threonine protein kinase PSSALRE). [Homo sapiens]

CDK5_M0UΞE Cell division protein kinase 5 (EC 2.7.1.-) (Tau protein kinase II catalytic subunit) (TPKII catalytic subunit)

(Serine/threonine protein kinase PSSALRE) (CRK6). [Mus musculus]

CDK5_RAT Cell division protein kinase 5 (EC 2.7.1.-) (Tau protein kinase II catalytic subunit) (TPKII catalytic subunit)

(Serine/threonine protein kinase PSSALRE). [Rattus norvegicus]

CDK6_HUMAN Cell division protein kinase 6 (EC 2.7.1.37) (Serine/threonine protein kinase PLSTIRE). [Homo sapiens] CDK9_HUMAN Cell division protein kinase 9 (EC 2.7.1.-) (Serine/threonine-protein kinase PITALRE) (C-2K). [Homo sapiens]

CHK1JHUMAN Serine/threonine-protein kinase Chkl (EC 2.7.1.-). [Homo sapiens] CHK2JHUMAN Serine/threonine-protein kinase Chk2 (EC 2.7.1.37) (Cdsl). [Homo sapiens] CNE3_HUMAIM Coplne III. [Homo sapiens] CSKP_HUMAN Peripheral plasma membrane protein CASK (EC 2.7.1.-) (hCASK) (Calcium/calmodulin-dependent serine protein kinase) (Lin-2 homolog). [Homo sapiens]

CSK_HUMAN Tyrosine-protein kinase CSK (EC 2.7.1.112) (C-SRC kinase) (Protein- tyrosine kinase CYL). [Homo sapiens] CSK_MOUSE Tyrosine-proteln kinase CSK (EC 2.7.1.112) (C-SRC kinase) (Protein- tyrosine kinase MPK-2). [Mus musculus]

CSK_RAT Tyrosine-protein kinase CSK (EC 2.7.1.112) (C-SRC kinase). [Rattus norvegicus]

DAPK_HUMAN Death-associated protein kinase 1 (EC 2.7.1.-) (DAP kinase 1). [Homo sapiens]

DCKl_MOUSE Serine/threonine-protein kinase DCAM L1 (EC 2.7.1.-) (Doublecortin- like and CAM kinase-like 1). [Mus musculus]

DYRA_HUMAN Dual-specificity tyrosine-phosphorylatioπ regulated kinase 1A (EC 2.7.1.-) (Protein kinase minibrain homolog) (MNBH) (HP86) (Dual specificity YAKl-related kinase). [Homo sapiens]

E2K2_HUMAN Interferon-induced, double-stranded RNA-activated protein kinase (EC 2.7.1.-) (Interferon-lnducible RNA- dependent protein kinase) (p68 kinase) (Pl/eIF-2A protein kinase). [Homo sapiens]

E2K2_M0USE Interferon-induced, double-stranded RNA-activated protein kinase (EC 2.7.1.-) (Interferon-inducible RNA- dependent protein kinase) (p68 kinase) (Pl/eIF-2A protein kinase) (Serine/threonine-protein kinase TIK).

[Mus musculus]

EF2K_HUMAN Elongation factor 2 kinase (EC 2.7.1.-) (eEF-2 kinase) (eEF-2K) (Calcium/calmodulin-dependent eukaryotic elongation factor-2 kinase). [Homo sapiens]

EF2K_RAT Elongation factor 2 kinase (EC 2.7.1.-) (eEF-2 kinase) (eEF-2K) (Calcium/calmodulin-dependent eukaryotic elongation factor-2 kinase). [Rattus norvegicus]

EGFRJHUMAN Epidermal growth factor receptor precursor (EC 2.7.1.112) (Receptor protein-tyrosine kinase ErbB-1).

[Homo sapiens]

EPA1_HUMAN Ephrin type-A receptor 1 precursor (EC 2.7.1.112) (Tyrosine-proteln kinase receptor EPH). [Homo sapiens] EPA2_HUMAN Ephrin type-A receptor 2 precursor (EC 2.7.1.112) (Tyrosine-proteln kinase receptor ECK) (Epithelial cell kinase). [Homo sapiens]

EPA7_HUMAN Ephrin type-A receptor 7 precursor (EC 2.7.1.112) (Tyrosine-protein kinase receptor EHK-3) (Eph homology kinase-3) (Receptor protein- tyrosine kinase HEK11). [Homo sapiens]

FAK1_HUMAN Focal adhesion kinase 1 (EC 2.7.1.112) (FADK 1) (ppl25FAK) (Protein- tyrosine kinase 2). [Homo sapiens] FAK2_HUMAN Protein tyrosine kinase 2 beta (EC 2.7.1.112) (Focal adhesion kinase 2) (FADK 2) (Proline-rich tyrosine kinase 2) (Cell adhesion kinase beta) (CAK beta) (Calcium-dependent tyrosine kinase) (CADTK) (Related adhesion focal tyrosine kinase). [Homo sapiens]

FER_HUMAN Proto-oncogene tyrosine-proteln kinase FER (EC 2.7.1.112) (p94-FER) (c-FER). [Homo sapiens] FES_HUMAN Proto-oncogene tyrosine-protein kinase FES/FPS (EC 2.7.1.112) (C-FES). [Homo sapiens] FGR1_M0USE Basic fibroblast growth factor receptor 1 precursor (EC 2.7.1.112) (FGFR-1) (bFGF-R) (MFR). [Mus musculus]

FGR_HUMAN Proto-oncogene tyrosine-protein kinase FGR (EC 2.7.1.112) (P55-FGR) (C-FGR). [Homo sapiens]

FLK_RAT Tyrosine-protein kinase FLK (EC 2.7.1.112) (Fragment). [Rattus norvegicus]

GRK5_RAT G protein-coupled receptor kinase GRK5 (EC 2.7.1.-) (G-proteln-coupled receptor kinase 5). [Rattus norvegicus]

HCK_HUMAN Tyrosine-protein kinase HCK (EC 2.7.1.112) (P59-HCK and P60-HCK) IKKA_HUMAN Inhibitor of nuclear factor kappa-B kinase alpha subunit (EC 2.7.1.-) (I kappa-B kinase alpha) (IkBKA)

(IKK-alpha) (IKK-A) (IkappaB kinase) (I-kappa-B kinase 1) (IKK1) (Conserved helix-loop-hellx ubiquitous kinase) (Nuclear factor NFkappaB inhibitor kinas

IKKA_MOUSE Inhibitor of nuclear factor kappa-B kinase alpha subunit (EC 2.7.1.-) (I kappa-B kinase alpha) (IkBKA)

(IKK-alpha) (IKK-A) (IkappaB kinase) (I-kappa-B kinase 1) (IKK1) (Conserved hellx-loop-helix ubiquitous kinase) (Nuclear factor NFkappaB inhibitor kinas IKKB_HUMAN Inhibitor of nuclear factor kappa B kinase beta subunit (EC 2.7.1.-) (I-kappa-B-kinase beta) (IkBKB) (IKK- beta) (IKK-B) (I-kappa-B kinase 2) (IKK2) (Nuclear factor NF-kappa-B inhibitor kinase beta) (NFKBIKB).

[Homo sapiens] IKKB_MOUSE Inhibitor of nuclear factor kappa B kinase beta subunit (EC 2.7.1.-) (I-kappa-B-kinase beta) (IkBKB) (IKK- beta) (IKK-B) (I-kappa-B kinase 2) (IKK2) (Nuclear factor NF-kappa-B Inhibitor kinase beta) (NFKBIKB).

[Mus musculus] IKKB_RAT Inhibitor of nuclear factor kappa B kinase beta subunit (EC 2.7.1.-) (I-kappa-B-kinase beta) (IkBKB) (IKK- beta) (IKK-B) (I-kappa-B kinase 2) (IKK2) (Nuclear factor NF-kappa-B inhibitor kinase beta) (NFKBIKB).

[Rattus norvegicus] ILK1_HUMAN Integrin-linked protein kinase 1 (EC 2.7.1.-) (ILK-1) (59 kDa serine/threonine protein kinase) (p59ILK).

[Homo sapiens] ILK_MOUSE Integrin-linked protein kinase (EC 2.7.1.-). [Mus musculus]

INSR_HUMAN Insulin receptor precursor (EC 2.7.1.112) (IR) (CD220 antigen). [Homo sapiens] IRA1_HUMAN Interleukin-1 receptor-associated kinase 1 (EC 2.7.1.-) (IRAK-1). [Homo sapiens] -AK1_HUMAN Tyrosine-proteln kinase JAK1 (EC 2.7.1.112) (Janus kinase 1) (JAK-1). [Homo sapiens] JAK2_MOUSE Tyrosine-proteln kinase JAK2 (EC 2.7.1.112) (Janus kinase 2) (JAK-2). [Mus musculus] JAK3_HUMAN Tyrosine-protein kinase JAK3 (EC 2.7.1.112) (Janus kinase 3) (JAK-3) (Leukocyte janus kinase) (L-JAK).

[Homo sapiens] JAK3_RAT Tyrosine-protein kinase JAK3 (EC 2.7.1.112) (Janus kinase 3) (JAK-3). [Rattus norvegicus]

K6A1_HUMAN Ribosomal protein S6 kinase alpha 1 (EC 2.7.1.37) (S6K-alpha 1) (90 kDa ribosomal protein S6 kinase 1)

(P90-RSK 1) (Ribosomal S6 kinase 1) (RSK-1) (pp90RSKl). [Homo sapiens] K6A1_RAT Ribosomal protein S6 kinase alpha 1 (EC 2.7.1.37) (S6K-alpha 1) (90 kDa ribosomal protein S6 kinase 1)

(p90-RSK 1) (Ribosomal S6 kinase 1) (RSK-1) (pp90RSKl). [Rattus norvegicus] K6A2_HUMAN Ribosomal protein S6 kinase alpha 2 (EC 2.7.1.37) (S6K-alpha 2) (90 kDa ribosomal protein S6 kinase 2)

(p90-RSK 2) (Ribosomal S6 kinase 3) (RSK-3) (pp90RSK3). [Homo sapiens] K6A2_MOUSE Ribosomal protein S6 kinase alpha 2 (EC 2.7.1.37) (S6K-alpha 2) (90 kDa ribosomal protein S6 kinase 2)

(p90-RSK 2) (Ribosomal S6 kinase 3) (RSK-3) (pp90RSK3) (Proteln-tyrosine kinase Mpk-9). [Mus musculus] K6A3_HUMAN Ribosomal protein S6 kinase alpha 3 (EC 2.7.1.-) (S6K-alpha 3) K6A3_MOUSE Ribosomal protein S6 kinase alpha 3 (EC 2.7.1.37) (S6K-alpha 3) (90 kDa ribosomal protein S6 kinase 3)

(p90-RSK 3) (Ribosomal S6 kinase 2) (RSK-2) (pp90RSK2). [Mus musculus] K6A6_HUMAN Ribosomal protein S6 kinase alpha 6 (EC 2.7.1.37) (S6K-alpha 6) (90 kDa ribosomal protein S6 kinase 6)

(P90-RSK 6) (Ribosomal S6 kinase 4) (RSK-4) (pp90RSK4). [Homo sapiens] K6B1_HUMAN Ribosomal protein S6 kinase (EC 2.7.1.-) (S6K) (p70-S6K). [Homo sapiens] K6B1_RAT Ribosomal protein S6 kinase I (EC 2.7.1.-) (S6K) (P70-S6K). [Rattus norvegicus]

K6B2_MOUSE Ribosomal protein S6 kinase beta 2 (EC 2.7.1.-) (S6K-beta 2) (70 kDa ribosomal protein S6 kinase 2)

(P70-S6KB) (p70 ribosomal S6 kinase beta) (p70 S6Kbeta) (S6K2). [Mus musculus] KC1A_RAT Casein kinase I, alpha isoform (EC 2.7.1.-) (CKI-alpha) (CK1). [Rattus norvegicus]

KC21JHUMAN Casein kinase II, alpha chain (CK II) (EC 2.7.1.37). [Homo sapiens] KC22_HUMAN Casein kinase II, alpha' chain (CK II) (EC 2.7.1.37). [Homo sapiens] KC2BJHUMAN Casein kinase II beta chain (CK II) (Phosvitin) (G5a). [Homo sapiens]

KCCIJHUMAN Calcium/calmodulin-dependent protein kinase type I (EC 2.7.1.123) (CAM kinase I). [Homo sapiens] KCC4_HUMAN Calcium/calmodulin-dependent protein kinase type IV catalytic chain (EC 2.7.1.123) (CAM kinase-GR)

(CaMK IV). [Homo sapiens] KCC4_MOUSE Calcium/calmodulin-dependent protein kinase type IV catalytic chain (EC 2.7.1.123) (CAM kinase-GR)

(CaMK IV). [Mus musculus] KCC4_RAT Calcium/calmodulin-dependent protein kinase type IV catalytic chain (EC 2.7.1.123) (CAM klnase-GR)

(CaMK IV) (Calspermin). [Rattus norvegicus] KCCB_MOUSE Calcium/calmodulin-dependent protein kinase type II beta chain (EC 2.7.1.123) (CaM-kinase II beta chain)

(CaM kinase II beta subunit) (CaMK-II beta subunit). [Mus musculus] KCCG_HUMAN Calcium/calmodulin-dependent protein kinase type II gamma chain (EC 2.7.1.123) (CaM-kinase II gamma chain) (CaM kinase II gamma subunit) (CaMK-II gamma subunit) (Fragment). [Homo sapiens] KCCG_RAT Calcium/calmodulin-dependent protein kinase type II gamma chain (EC 2.7.1.123) (CaM-kinase II gamma chain) (CaM kinase II gamma subunit) (CaMK-II gamma subunit). [Rattus norvegicus] KCH1_HUMAN Potassium voltage-gated channel subfamily H member 1 (Ether-a-go-go potassium channel 1) (hEAGl) (h- eag). [Homo sapiens] KG3AJHUMAN Glycogen synthase kinase-3 alpha (EC 2.7.1.37) (GSK-3 alpha). [Homo sapiens] KG3A_RAT Glycogen synthase kinase-3 alpha (EC 2.7.1.37) (GSK-3 alpha) (Factor A) (FA). [Rattus norvegicus]

KG3BJHUMAN Glycogen synthase klnase-3 beta (EC 2.7.1.37) (GSK-3 beta). [Homo sapiens] KG3B_MOUSE Glycogen synthase kinase-3 beta (EC 2.7.1.37) (GSK-3 beta). [Mus musculus] KISTJHUMAN Serine/threonine-protein kinase Kist (EC 2.7.1.37) (Kinase interacting with stathmin). [Homo sapiens] KMLSJHUMAN Myosln light chain kinase, smooth muscle and non-muscle Isozymes (EC 2.7.1.117) (MLCK) [Contains:

Telokin (Kinase related protein) (KRP)]. [Homo sapiens] KPBH_HUMAN Phosphorylase B kinase gamma catalytic chain, testis/liver Isoform (EC 2.7.1.38) (PHK-gamma-T)

(Phosphorylase kinase gamma subunit 2) (PSK-C3). [Homo sapiens] KPCA_HUMAN Protein kinase C, alpha type (EC 2.7.1.37) (PKC-alpha) (PKC-A). [Homo sapiens] KPCA_RAT Protein kinase C, alpha type (EC 2.7.1.37) (PKC-alpha) (PKC-A). [Rattus norvegicus]

KPCB_HUMAN Protein kinase C, beta type (EC 2.7.1.37) (PKC-beta) (PKC-B). [Homo sapiens] KPCD HUMAN Protein kinase C, delta type (EC 2.7.1.-) (nPKC-delta). [Homo sapiens] KPCG_MOUSE Protein kinase C, gamma type (EC 2.7.1.37) (PKC-gamma). [Mus musculus] KPCI_HUMAN Protein kinase C, iota type (EC 2.7.1.37) (nPKC-iota) (Atypical protein kinase C-lamda/iota) (aPKC- lambda/iota). [Homo sapiens] KPCI_MOUSE Protein kinase C, iota type (EC 2.7.1.-) (nPKC-iota) (Protein k

KPCM_HUMAN Protein kinase C, mu type (EC 2.7.1.-) (nPKC-mu) (Protein kinase D). [Homo sapiens] KPCT_HUMAN Protein kinase C, theta type (EC 2.7.1.-) (nPKC-theta). [Homo sapiens] KPCZ_RAT Protein kinase C, zeta type (EC 2.7.1.37) (nPKC-zeta). [Rattus norvegicus]

KPSH_HUMAN Serine/threonine-protein kinase HI (EC 2.7.1.37) (PSK-H1). [Homo sapiens] KROS_HUMAN Proto-oncogene tyrosine-protein kinase ROS precursor (EC 2.7.1.112) (c-ros-1). [Homo sapiens] KSYK_MOUSE Tyrosine-protein kinase SYK (EC 2.7.1.112) (Spleen tyrosine kinase). [Mus musculus] LCK HUMAN Proto-oncogene tyrosine-protein kinase LCK (EC 2.7.1.112) (P56-LCK) (LSK) (T cell-specific protein- tyrosine kinase). [Homo sapiens] LTBL_HUMAN Latent transforming growth factor beta binding protein, isoform IL precursor (LTBP-1) (Transforming growth factor beta-1 binding protein 1) (TGF-betal-BP-1). [Homo sapiens] M3K1_HUMAN Mitogen-activated protein kinase kinase kinase 1 (EC 2.7.1.-) ( M3K2_HUMAN Mitogen-activated protein kinase kinase kinase 2 (EC 2.7.1.-) (MAPK/ERK kinase kinase 2) (MEK kinase 2)

(MEKK 2). [Homo sapiens] M3K3_HUMAN Mitogen-activated protein kinase kinase kinase 3 (EC 2.7.1.-) (MAPK/ERK kinase kinase 3) (MEK kinase 3)

(MEKK 3). [Homo sapiens] M3K4_HUMAN Mitogen-activated protein kinase kinase kinase 4 (EC 2.7.1.-) (MAPK/ERK kinase kinase 4) (MEK kinase 4)

(MEKK 4) (MAP three kinase 1). [Homo sapiens] M3K5_HUMAN Mitogen-activated protein kinase kinase kinase 5 (EC 2.7.1.-) (MAPK/ERK kinase kinase 5) (MEK kinase 5)

(MEKK 5) (Apoptosis signal- regulating kinase 1) (ASK-1). [Homo sapiens] M4K2_HUMAN Mitogen-activated protein kinase kinase kinase kinase 2 (EC 2.7 M4K2_MOUSE Mitogen-activated protein kinase kinase kinase kinase 2 (EC 2.7.1.37) (MAPK/ERK kinase kinase kinase 2)

(MEK kinase kinase 2) (MEKKK 2) (Germinal center kinase) (GCK) (Rab8 interacting protein). [Mus musculus] MAK_HUMAN Seπne/threonlne-protein kinase MAK (EC 2.7.1.-) (Male germ cell- associated kinase). [Homo sapiens] MET HUMAN Hepatocyte growth factor receptor precursor (EC 2.7.1.112) (Met proto- oncogene tyrosine kinase) (c-met)

(HGF receptor) (HGF-SF receptor). [Homo sapiens] MK01_BOVIN Mitogen-activated protein kinase 1 (EC 2.7.1.37) (Extracellular signal-regulated kinase 2) (ERK-2)

(Mitogen-activated protein kinase 2) (MAP kinase 2) (MAPK 2) (p42-MAPK) (ERT1). [Bos taurus] MK01_HUMAN Mitogen-activated protein kinase 1 (EC 2.7.1.37) (Extracellular signal-regulated kinase 2) (ERK-2)

(Mitogen-activated protein kinase 2) (MAP kinase 2) (MAPK 2) (p42-MAPK) (ERT1). [Homo sapiens] MK01_MOUSE Mitogen-activated protein kinase 1 (EC 2.7.1.37) (Extracellular signal-regulated kinase 2) (ERK-2)

(Mitogen-activated protein kinase 2) (MAP kinase 2) (MAPK 2) (p42-MAPK) (ERT1). [Mus musculus] MK03JHUMAN Mitogen-activated protein kinase 3 (EC 2 7.1.37) (Extracellular signal-regulated kinase 1) (ERK-1) (Insulin- stimulated MAP2 kinase) (MAP kinase 1) (MAPK 1) (p44-ERKl) (ERT2) (p44-MAPK) (Microtubule- associated protein-2 kinase). [Homo sapiens] MK03_MOUSE Mitogen-activated protein kinase 3 (EC 2.7.1.37) (Extracellular signal-regulated kinase 1) (ERK-1) (Insulin- stimulated MAP2 kinase) (MAP kinase 1) (MAPK 1) (p44-ERKl) (ERT2) (p44-MAPK) (Microtubule- assoclated proteιn-2 kinase) (MNK1) (Fragments). [Mus mu MK03_RAT Mitogen-activated protein kinase 3 (EC 2.7.1.37) (Extracellular signal-regulated kinase 1) (ERK-1) (Insulin- stimulated MAP2 kinase) (MAP kinase 1) (MAPK 1) (p44-ERKl) (ERT2) (p44-MAPK) (Microtubuie- associated proteιn-2 kinase) (MNK1) [Rattus norvegicus] MK08_HUMAN Mitogen-activated protein kinase 8 (EC 2.7.1.37) (Stress-activated protein kinase JNK1) (c-Jun N-terminal kinase 1) (JNK-46). [Homo sapiens] MK08_MOUSE Mitogen-activated protein kinase 8 (EC 2.7.1.37) (Stress-activated protein kinase JNK1) (c-Jun N-terminal kinase 1). [Mus musculus] MK12_HUMAN Mitogen-activated protein kinase 12 (EC 2.7.1.37) (Extracellular signal-regulated kinase 6) (ERK-6) (ERK5)

(Stress-activated protein klnase-3) (Mitogen-activated protein kinase p38 gamma) (MAP kinase p38 gamma). [Homo sapiens] MK14_HUMAN Mitogen-activated protein kinase 14 (EC 2.7.1.37) (Mitogen-activated protein kinase p38aipha) (MAP kinase p38alpha) (Cytokine suppresslve anti-inflammatory drug binding protein) (CSAID binding protein)

(CSBP) (MAX-interacting protein 2) (MAP kinase MXI2) MKK2JHUMAN MAP kinase-activated protein kinase 2 (EC 2.7.1.-) (MAPK-actlvated protein kinase 2) (MAPKAP kinase 2)

(MAPKAPK-2). [Homo sapiens] MPK1_RABIT Dual specificity mitogen-activated protein kinase kinase 1 (EC 2.7.1.-) (MAP kinase kinase 1) (MAPKK 1)

(ERK activator kinase 1) (MAPK/ERK kinase 1) (MEK1). [Oryctolagus cuniculus] MPK2_HUMAN Dual specificity mitogen-activated protein kinase kinase 2 (EC 2.7 1.-) (MAP kinase kinase 2) (MAPKK 2)

(ERK activator kinase 2) (MAPK/ERK kinase 2) (MEK2). [Homo sapiens] MPK2_MOUSE Dual specificity mitogen-activated protein kinase kinase 2 (EC 2.7.1.-) (MAP kinase kinase 2) (MAPKK 2)

(ERK activator kinase 2) (MAPK/ERK kinase 2) (MEK2). [Mus musculus] MPK2_RAT Dual specificity mitogen-activated protein kinase kinase 2 (EC 2.7.1.-) (MAP kinase kinase 2) (MAPKK 2)

(ERK activator kinase 2) (MAPK/ERK kinase 2) (MEK2). [Rattus norvegicus] MPK3_HUMAN Dual specificity mitogen-activated protein kinase kinase 3 (EC 2.7 1.-) (MAP kinase kinase 3) (MAPKK 3)

(MAPK/ERK kinase 3). [Homo sapiens] MPK4JHUMAN Dual specificity mitogen-activated protein kinase kinase 4 (EC 2.7.1.-) (MAP kinase kinase 4) (JNK activating kinase 1) (c-Jun N- terminal kinase kinase 1) (JNKK) (SAPK/ERK kinase 1) (SEK1). [Homo sapiens] MPK4_MOUSE Dual specificity mitogen-activated protein kinase kinase 4 (EC 2.7.1.-) (MAP kinase kinase 4) (MAPKK 4)

(MAPK/ERK kinase 4) (JNK activating kinase 1) (C-JUN N-terminal kinase kinase 1) (JNK kinase 1) (JNKK

1) (SAPK/ERK kinase 1) (SEK1). [Mus musculus] MPK5_ARATH Mitogen-activated protein kinase homolog MPK6_HUMAN Dual specificity mitogen-activated protein kinase kinase 6 (EC 2.7 1.-) (MAP kinase kinase 6) (MAPKK 6)

(MAPK/ERK kinase 6) (SAPKK3). [Homo sapiens] MPK6_MOUSE Dual specificity mitogen-activated protein kinase kinase 6 (EC 2.7.1.-) (MAP kinase kinase 6) (MAPKK 6)

(MAPK/ERK kinase 6) (SAPKK3). [Mus musculus] MRK4JHUMAN MAP/microtubule affinity-regulating kinase 4 (EC 2.7.1.27) (MAP/microtubule affinity-regulating kinase like

1). [Homo sapiens] NRP1--HUMAN Neuropilln-1 precursor (Vascular endothelial cell growth factor 088664 Serine/threonine protein kinase TAOl. [Rattus norvegicus]

PAK2_HUMAN Serine/threonine-protein kinase PAK 2 (EC 2.7.1.-) (p21-actlvated kinase 2) (PAK-2) (PAK65) (Gamma-

PAK) (S6/H4 kinase). [Homo sapiens] PDKIJHUMAN [Pyruvate dehydrogenase [llpoamide]] kinase isozyme 1, mitochondrial precursor (EC 2.7.1.99) (Pyruvate dehydrogenase kinase Isoform 1). [Homo sapiens] PDK3_HUMAN [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 3, mitochondrial precursor (EC 2.7.1.99) (Pyruvate dehydrogenase kinase isoform 3). [Homo sapiens] PDK4_MOUSE [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4, mitochondrial precursor (EC 2.7.1.99) (Pyruvate dehydrogenase kinase isoform 4). [Mus musculus] PDPK_HUMAN 3-phosphoinositide dependent protein kinase-1 (EC 2.7.1.37) (hPDKl). [Homo sapiens]

PGDRJHUMAN Beta platelet-derived growth factor receptor precursor (EC 2.7.1.112) (PDGF-R-beta) (CD140b antigen).

[Homo sapiens] PGDS_RAT Alpha platelet-derived growth factor receptor precursor (EC 2.7.1.112) (PDGF-R-alpha). [Rattus norvegicus] PKL1_HUMAN Protein kinase C-like 1 (EC 2.7.1.'-) (Protein-kinase C-related

PKL2_HUMAN Protein kinase C-like 2 (EC 2.7.1.-) (Protein-kinase C-related kinase 2). [Homo sapiens] PKX1_HUMAN Protein kinase PKX1 (EC 2.7.1.-). [Homo sapiens] PLK1_HUMAN Serine/threonine-protein kinase PLK (EC 2.7.1.-) (PLK-1) (Serine- threonine protein kinase 13) (STPK13).

[Homo sapiens] PLKl_MOUSE Serine/threonine-protein kinase PLK (EC 2.7.1.-) (PLK-1) (Serine- threonine protein kinase 13) (STPK13).

[Mus musculus] PRKD_HUMAN DNA-dependent protein kinase catalytic subunit (EC 2.7.1.37) (DNA- PKcs) (DNPK1). [Homo sapiens] PRPKJHUMAN p53-related protein kinase (EC 2.7.1.-) (Nori-2). [Homo sapiens]

PTK7JHUMAN Tyrosine-protein kinase-like 7 precursor (Colon carcinoma kinase-4) (CCK.-4). [Homo sapiens] Q63709 Fibroblast growth factor receptor subtype 4. [Rattus rattus]

Q8IWY7 Tau-tubulin kinase. [Homo sapiens]

RET_HUMAN Proto-oncogene tyrosine-protein kinase receptor ret precursor (EC 2.7.1.112) (C-ret). [Homo sapiens] RIK1_HUMAN Receptor-interacting serine/threonine protein kinase 2 (EC 2.7.1.37) (Serine/threonine protein kinase RIP)

(Cell death protein RIP) (Receptor interacting protein). [Homo sapiens] RIK2_HUMAN Receptor-interacting serine/threonine protein kinase 2 (EC 2.7.1.37) (RIP-like Interacting CLARP kinase)

(Receptor-Interacting protein 2) (RIP-2) (CARD-containlng interleukin-1 beta converting enzyme associated kinase) (CARD-containlng IL-1 beta ICE-kinas RIK3_MOUSE Receptor-interacting serine/threonine protein kinase 3 (EC 2.7.1.37) (RIP-like protein kinase 3) (Receptor- interacting protein 3) (RIP-3) (mRIP3). [Mus musculus] RN5A_HUMAN 2-5A-dependent ribonuclease (EC 3.1.26.-) (2-5A-dependent RNase) (Ribonuclease L) (RNase L)

(Ribonuclease 4). [Homo sapiens] SGK1_HUMAN Serine/threonine-protein kinase Sgkl (EC 2.7.1.37) (Serum/glucocorticoid-regulated kinase 1). [Homo sapiens] SGK3JHUMAN Serine/threonine-protein kinase Sgk3 (EC 2.7.1.37) (Serum/glucocorticoid regulated kinase 3)

(Serum/glucocorticold regulated kinase-like). [Homo sapiens] SNKJHUMAN Serine/threonine-protein kinase SNK (EC 2.7.1.-) (Serum induclble kinase). [Homo sapiens] SPAK_RAT STE20/SPSl-related proline-alanine rich protein kinase (EC 2.7.1.-) (Ste-20 related kinase)

(Serine/threonine-protein kinase 39) (Pancreatic serine/threonine kinase) (PS/TK) (PSTK1). [Rattus norvegicus] ST24_HUMAN Serine/threonine protein kinase 24 (EC 2.7.1.37) (STE20-llke kinase MST3) (MST-3) (Mammalian STE20- like protein kinase 3). [Homo sapiens] ST25_HUMAN Serine/threonine protein kinase 25 (EC 2.7.1.37) (Sterile 20/oxidant stress-response kinase 1)

(Ste20/oxidant stress response kinase-1) (SOK-1) (Ste20-like kinase). [Homo sapiens] STK3_HUMAN Serine/threonine protein kinase 3 (EC 2.7.1.37) (STE20-like kinase MST2) (MST-2) (Mammalian STE20-like protein kinase 2) (Serine/threonine protein kinase Krs-1). [Homo sapiens] STK4_HUMAN Serine/threonine protein kinase 4 (EC 2.7.1.37) (STE20-like kinase MSTl) (MST-1) (Mammalian STE20-like protein kinase 1) (Serine/threonine protein kinase Krs-2). [Homo sapiens] STK6JHUMAN Serine/threonine kinase 6 (EC 2.7.1.37) (Serine/threonine kinase 15) (Aurora/IPLl-related kinase 1)

(Aurora-related kinase 1) (hARKl) (Aurora-A) (Breast-tumor-amplified kinase). [Homo sapiens] STKA_HUMAN Serine/threonine protein kinase 10 (EC 2.7.1.37) (Lymphocyte-oriented kinase). [Homo sapiens] STKA_MOUSE Serine/threonine protein kinase 10 (EC 2.7.1.37) (Lymphocyte-oriented kinase). [Mus musculus] T2D1_HUMAN Transcription initiation factor TFIID 250 kDa subunit (TAFII-250) (TAFII250) (TBP-assoclated factor 250 kDa) (P250) (Cell cycle gene 1 protein). [Homo sapiens] TNIK_HUMAN TRAF2 and NCK interacting kinase (EC 2.7.1.37). [Homo sapiens] VGR2JHUMAN Vascular endothelial growth factor receptor 2 precursor (EC 2.7.1.112) (VEGFR-2) (Kinase insert domain receptor) (Proteln-tyrosine kinase receptor Flk-1). [Homo sapiens] WEE1_HUMAN Weel-like protein kinase (EC 2.7.1.112) ( EElhu). [Homo sapiens]

YES_HUMAN Proto-oncogene tyrosine-protein kinase YES (EC 2.7.1.112) (p61-YES) (C-YES). [Homo sapiens]

ZA70 HUMAN Tyrosine-protein kinase ZAP-70 (EC 2.7.1.112) (70 kDa zeta-associated protein) (Syk-related tyrosine kinase). [Homo sapiens]

Other kinases

ADK_HUMAN Adenoslne kinase (EC 2.7.1.20) (AK) (Adenoslne 5'-phosphotransferase). [Homo sapiens]

ADK_MOUSE Adenosine kinase (EC 2.7.1.20) (AK) (Adenoslne 5'-phosphotransferase) (Fragment). [Mus musculus]

DCK_HUMAN Deoxycytidine kinase (EC 2.7.1.74) (dCK). [Homo sapiens]

DCK_RAT Deoxycytidine kinase (EC 2.7.1.74) (dCK). [Rattus norvegicus]

DGK_HUMAN Deoxyguanoslne kinase, mitochondrial precursor (EC 2.7.1.113) (dGK). [Homo sapiens]

EKU_HUMAN Ethanolamine kinase (EC 2.7.1.82) (EKI). [Homo sapiens]

ER19_HUMAN Dlphosphomevalonate decarboxylase (EC 4.1.1.33) (Mevalonate pyrophosphate decarboxylase)

(Mevalonate (diphospho)decarboxylase). [Homo sapiens]

F263_HUMAN 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (6PF-2-K/Fru- 2,6-P2ASE brain/placenta-type isozyme) (IPFK-2) [Includes: 6- phosphofructo-2-klnase (EC 2.7.1.105); Fructose-2,6-bisphosphatase (EC

3.1.3.46)]. [Homo sapiens]

FRAPJHUMAN FKBP-rapamycin associated protein (FRAP) (Rapamycin target protein). [Homo sapiens] FYV1_HUMAN FYVE finger-containing phospholnositide kinase (EC 2.7.1.68) (1- phosphatldyllnositol-4-phosphate 5- kinase) (PIP5K) (PtdIns(4)P-5- kinase) (p235) (Fragment). [Homo sapiens]

HXKIJHUMAN Hexokinase, type I (EC 2.7.1.1) (HK I) (Brain form hexokinase). [Homo sapiens] K6PL_HUMAN 6-phosphofructokinase, liver type (EC 2.7.1.11) (Phosphofructokinase 1) (Phosphohexoklnase)

(Phosphofructo-1-klnase Isozyme B) (PFK-B). [Homo sapiens]

K6PL_MOUSE 6-phosphofructokinase, liver type (EC 2.7.1.11) (Phosphofructokinase 1) (Phosphohexoklnase)

(Phosphofructo-1-klnase isozyme B) (PFK-B). [Mus musculus]

K6PL_RAT 6-phosphofructoklnase, liver type (EC 2.7.1.11) (Phosphofructokinase 1) (Phosphohexoklnase)

(Phosphofructo-1-klnase isozyme B) (PFK-B). [Rattus norvegicus] K6PP_HUMAN 6-phosphofructokinase, type C (EC 2.7.1.11) (Phosphofructokinase 1) (Phosphohexoklnase)

(Phosphofructo-1-kinase isozyme C) (PFK-C) (6-phosphofructokinase, platelet type). [Homo sapiens] K6PP_MOUSE 6-phosphofructokinase, type C (EC 2.7.1.11) (Phosphofructokinase 1) (Phosphohexoklnase)

(Phosphofructo-1-klnase isozyme C) (PFK-C). [Mus musculus] KADl_BOVIN Adenylate kinase isoenzyme 1 (EC 2.7.4.3) (ATP-AMP transphosphoryiase) (AK1) (Myokinase). [Bos taurus] KAD1_HUMAN Adenylate kinase isoenzyme 1 (EC 2.7.4.3) (ATP-AMP transphosphoryiase) (AK1) (Myokinase). [Homo sapiens] KADl_MOUSE Adenylate kinase isoenzyme 1 (EC 2.7.4.3) (ATP-AMP transphosphoryiase) (AK1) (Myokinase). [Mus musculus] KAD1_RAT Adenylate kinase isoenzyme 1 (EC 2.7.4.3) (ATP-AMP transphosphoryiase) (AK1) (Myokinase). [Rattus norvegicus] KAD2_BOVIN Adenylate kinase isoenzyme 2, mitochondria] (EC 2.7.4.3) (ATP-AMP transphosphoryiase). [Bos taurus] KAD2_MOUSE Adenylate kinase isoenzyme 2, mitochondrial (EC 2.7.4.3) (ATP-AMP transphosphoryiase). [Mus musculus] KAD4_HUMAN Adenylate kinase Isoenzyme 4, mitochondrial (EC 2.7.4.3) (ATP-AMP transphosphoryiase). [Homo sapiens] KAD4_MOUSE Adenylate kinase isoenzyme 4, mitochondrial (EC 2.7.4.3) (ATP-AMP transphosphoryiase). [Mus musculus] KAD4_RAT Adenylate kinase isoenzyme 4, mitochondrial (EC 2.7.4.3) (ATP-AMP transphosphoryiase). [Rattus norvegicus] KAD5_MOUSE Adenylate kinase isoenzyme 5 (EC 2.7.4.3) (ATP-AMP transphosphoryiase). [Mus musculus] KCRB_MOUSE Creatlne kinase, B chain (EC 2.7.3.2) (B-CK). [Mus musculus] KCRM_MOUSE Creatlne kinase, M chain (EC 2.7.3.2) (M-CK). [Mus musculus] KCRS_RAT Creatlne kinase, sarcomeric mitochondrial precursor (EC 2.7.3.2) (S- MtCK) (Mib-CK) (Basic-type mitochondrial creatlne kinase). [Rattus norvegicus] KCY_HUMAN UMP-CMP kinase (EC 2.7.4.14) (Cytldylate kinase) (Deoxycytldylate kinase) (Cytidlne monophosphate kinase). [Homo sapiens] KCY_MOUSE UMP-CMP kinase (EC 2.7.4.14) (Cytldylate kinase) (Deoxycytidylate kinase) (Cytidlne monophosphate kinase). [Mus musculus] KDGA_HUMAN Diacylglycerol kinase, alpha (EC 2.7.1.107) (Diglyceride kinase) (DGK- alpha) (DAG kinase alpha) (80 kDa diacylglycerol kinase). [Homo sapiens] KDGG_HUMAN Diacylglycerol kinase, gamma (EC 2.7.1.107) (Diglyceride kinase) (DGK- gamma) (DAG kinase gamma).

[Homo sapiens] KICHJHUMAN Choline kinase (EC 2.7.1.32) (CK) (CHETK-alpha). [Homo sapiens] KIME_MOUSE Mevalonate kinase (EC 2.7.1.36) (MK). [Mus musculus] KIME_RAT Mevalonate kinase (EC 2.7.1.36) (MK). [Rattus norvegicus]

KPY1_FELCA Pyruvate kinase, Ml Isozyme (EC 2.7.1.40) (Pyruvate kinase muscle Isozyme). [Felis silvestris] KPY1_HUMAN Pyruvate kinase. Ml isozyme (EC 2.7.1.40) (Pyruvate kinase muscle isozyme) (Cytosolic thyroid hormone- binding protein) (CTHBP) (THBP1). [Homo sapiens] KPY2_MOUSE Pyruvate kinase, M2 Isozyme (EC 2.7.1.40). [Mus musculus] KPY2_RAT Pyruvate kinase, M2 isozyme (EC 2.7.1.40). [Rattus norvegicus]

KTHY_HUMAN Thymidylate kinase (EC 2.7.4.9) (dTMP kinase). [Homo sapiens]

MPP2_HUMAN MAGUK p55 subfamily member 2 (MPP2 protein) (Discs, large homolog 2). [Homo sapiens] NDK3_HUMAN Nucleoside diphosphate kinase 3 (EC 2.7.4.6) (NDK 3) (NDP kinase 3) (nm23-H3) (DR-nm23). [Homo sapiens] NDK8_HUMAN Putative nucleoside diphosphate kinase (EC 2.7.4.6) (NDK) (NDP kinase). [Homo sapiens] NDKA_HUMAN Nucleoside diphosphate kinase A (EC 2.7.4.6) (NDK A) (NDP kinase A) (Tumor metastatic process- associated protein) (Metastasis inhibition factor nm23) (nm23-Hl). [Homo sapiens] NDKA_RAT Nucleoside diphosphate kinase A (EC 2.7.4,6) (NDK A) (NDP kinase A) (Tumor metastatic process- associated protein) (Metastasis inhibition factor NM23). [Rattus norvegicus] NDKBJHUMAN Nucleoside diphosphate kinase B (EC 2.7.4.6) (NDK B) (NDP kinase B) (nm23-H2) (C-myc purine-binding transcription factor PUF). [Homo sapiens] NDKB_MOUSE Nucleoside diphosphate kinase B (EC 2.7.4.6) (NDK B) (NDP kinase B) (πm23-M2) (P18). [Mus musculus] NDKB_RAT Nucleoside diphosphate kinase B (EC 2.7.4.6) (NDK B) (NDP kinase B) (P18). [Rattus norvegicus]

O00334 Phosphatidylinositol 3-kinase delta catalytic subunit. [Homo sapiens]

P11B_HUMAN Phosphatidyllnositol-4,5-bisphosphate 3-kinase catalytic subunit, beta isoform (EC 2.7.1.153) (PI3-klnase pllO subunit beta) (PtdIns-3-kinase pllO) (PI3K) (PI3Kbeta). [Homo sapiens] P11G_HUMAN Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit, gamma isoform (EC 2.7.1.153) (PI3- kinase pllO subunit gamma) (Ptdlns- 3-kinase pllO) (PI3K) (PI3Kgamma). [Homo sapiens] PllG_MOUSE Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit, gamma isoform (EC 2.7.1.153) (PI3- kinase pllO subunit gamma) (Ptdlns- 3-kinase pllO) (PI3K) (PI3Kgamma). [Mus musculus] P5CSJHUMAN Delta l-pyrroline-5-carboxylate synthetase (P5CS) [Includes: Glutamate 5-kinase (EC 2.7,2.11) (Gamma- glutamyl kinase) (GK); Gamma-glutamyl phosphate reductase (GPR) (EC 1.2.1.41) (Glutamate-5- semlaldehyde dehydrogenase) (Glutamyl-gamma-semlaldehyde dehydr P85B_HUMAN Phosphatidylinositol 3-klnase regulatory beta subunit (PI3-kinase p85-beta subunit) (PtdIns-3-kinase p85- beta). [Homo sapiens] PDK1_RAT [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial precursor (EC 2.7.1.99) (Pyruvate dehydrogenase kinase isoform 1) (PDK P48). [Rattus norvegicus] PGK1_HUMAN Phosphoglycerate kinase 1 (EC 2.7.2.3) (Primer recognition protein 2) (PRP 2). [Homo sapiens] PGK2_MOUSE Phosphoglycerate kinase, testis specific (EC 2.7.2.3). [Mus musculus] PGK_SCHMA Phosphoglycerate kinase PI52_HUMAN Phosphatldylinositol-4-phosphate 5-kinase type II alpha (EC 2.7.1.149) (PIP5KII-alpha) (1- phosphatidylinosltol-4-phosphate 5-kinase) (PtdIns(4)P-5-kinase B isoform) (Diphosphoinositlde kinase).

[Homo sapiens] PI52_MOUSE Phosphatidylinositol-4-phosphate 5-kinase type II alpha (EC 2.7.1.149) (PIP5KII-alpha) (1- phosphatidylinositol-4-phosphate 5-klnase) (PtdIns(4)P-5-klnase B Isoform) (Diphosphoinositlde kinase).

[Mus musculus] PK3G_MOUSE Phosphatidyllnositol-4-phosphate 3-klnase C2 domain-containing gamma polypeptide (EC 2.7.1.154)

(Phosphoinositlde 3-Klnase-C2-gamma) (PtdIns-3-kinase C2 gamma) (PI3K-C2gamma). [Mus musculus] PPCC_RAT Phosphoenolpyruvate carboxykinase, cytosolic [GTP] (EC 4.1.1.32) (Phosphoenolpyruvate carboxylase)

(PEPCK-C). [Rattus norvegicus] PPNKJHUMAN Putative inorganic polyphosphate/ATP-NAD kinase (EC 2.7.1.23) (Poly(P)/ATP NAD kinase). [Homo sapiens] RBSK_HUMAN Ribokmase (EC 2.7.1.15). [Homo sapiens] UDP1JHUMAN UTP-glucose-1-phosphate uπdylyltransferase 1 (EC 2.7.7.9) (UDP- glucose pyrophosphorylase 1) (UDPGP

1) (UGPase 1). [Homo sapiens] UDP2_BOVIN UTP~glucose-l-phosphate uπdylyltransferase 2 (EC 2.7.7.9) (UDP- glucose pyrophosphorylase 2) (UDPGP

2) (UGPase 2). [Bos taurus] URL1_HUMAN Uridine kinase-like 1. [Homo sapiens]

ATPases

A10B_HUMAN Potential phospholipld-transportlng ATPase VB (EC 3.6.3.1). [Homo sapiens]

A11AJHUMAN Potential phospholipld-transportlng ATPase IH (EC 3.6.3.1) (ATPase class I type 11A) (ATPase IS). [Homo sapiens] A1A1_HUMAN Sodium/potassium-transporting ATPase alpha-1 chain precursor (EC 3.6.3.9) (Sodium pump 1) (Na+/K+

ATPase 1). [Homo sapiens] A1A1_RAT Sodium/potassium-transporting ATPase alpha-1 chain precursor (EC 3.6.3.9) (Sodium pump 1) (Na+/K+

ATPase 1). [Rattus norvegicus] A1A4JHUMAN Sodium/potassium-transporting ATPase alpha-4 chain (EC 3.6.3.9) (Sodium pump 4) (Na+/K+ ATPase 4).

[Homo sapiens] A8A1_HUMAN Potential phospholipld-transportlng ATPase IA (EC 3.6.3.1) (Chromaffin granule ATPase II) (ATPase class I type 8A member 1). [Homo sapiens] AB10_HUMAN ATP-binding cassette, sub-family B, member 10, mitochondrial precursor (ATP-binding cassette transporter

10) (ABC transporter 10 protein) (Mitochondrial ATP-binding cassette 2) (M-ABC2). [Homo sapiens] AB11_HUMAN Bile salt export pump (ATP-binding cassette, sub-family B, member 11). [Homo sapiens] AB11_RAT Bile salt export pump (ATP-binding cassette, sub-family B, member 11) (Sister of P-glycoprotein). [Rattus norvegicus] ABCl_MOUSE ATP-binding cassette, sub-family A, member 1 (ATP-binding cassette transporter 1) (ATP-binding cassette

1) (ABC-1). [Mus musculus] ABC7JHUMAN ATP-binding cassette, sub-family B, member 7, mitochondrial precursor (ATP-binding cassette transporter

7) (ABC transporter 7 protein). [Homo sapiens] ABCR_HUMAN Retinal-specific ATP-binding cassette transporter (RIM ABC transporter) (RIM protein) (RMP) (Stargardt disease protein). [Homo sapiens] ABD3_HUMAN ATP-binding cassette, sub-family D, member 3 (70 kDa peroxisomal membrane protein) (PMP70). [Homo sapiens] ABG5_HUMAN ATP-binding cassette, sub-family G, member 5 (Sterolιn-1). [Homo sapiens] ACA1_ARATH Calcium-transporting ATPase 1, plasma mem

ACIN_HUMAN Apoptotic chromatin condensation inducer in the nucleus (Acinus). [Homo sapiens] ALA8_ARATH Potential phospholipid-transporting ATPas ARSIJHUMAN Arsenical pump-driving ATPase (EC 3.6 3.16) (Arsenite-translocating ATPase) (Arsenical resistance ATPase)

(Arsenite-transporting ATPase) (ARSA) (ASNA-I). [Homo sapiens] ARSl_MOUSE Arsenical pump-driving ATPase (EC 3 6 3 16) (Arsenite-translocating ATPase) (Arsenical resistance ATPase)

(Arsenite-transporting ATPase) (ARSA) [Mus musculus] AT7AJHUMAN Copper-transporting ATPase 1 (EC 3.6.3.4) (Copper pump 1) (Menkes disease-associated protein). [Homo sapiens] AT7BJHUMAN Copper-transporting ATPase 2 (EC 3.6.3.4) (Copper pump 2) (Wilson disease-associated protein). [Homo sapiens] ATA1_HUMAN Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 (EC 3.6.3 8) (Calcium pump 1) (SERCA1) (SR

Ca(2+)-ATPase 1) (Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform) (Endoplasmic reticulum class 1/2 Ca(2+) ATPase). ATA1_RABIT Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 (EC 3.6.3.8) (Calcium pump 1) (SERCA1) (SR

Ca(2+)-ATPase 1) (Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle

Isoform) (Endoplasmic reticulum class 1/2 Ca(2+) ATPase). ATA1_RAT Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 (EC 3.6.3.8) (Calcium pump 1) (SERCA1) (SR

Ca(2+)-ATPase 1) (Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform) (Endoplasmic reticulum class 1/2 Ca(2+) ATPase). ATA2_HUMAN Sarcoplasmic/endoplasmic reticulum calcium ATPase 2 (EC 3.6.3.8) (Calcium pump 2) (SERCA2) (SR

Ca(2+)-ATPase 2) (Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform) (Endoplasmic reticulum class 1/2 Ca(2+) ATPase). ATA2_MOUSE Sarcoplasmic/endoplasmic reticulum calcium ATPase 2 (EC 3.6.3.8) (Calcium pump 2) (SERCA2) (SR

Ca(2+)-ATPase 2) (Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform) (Endoplasmic reticulum class 1/2 Ca(2+) ATPase). ATA2_RAT Sarcoplasmic/endoplasmic reticulum calcium ATPase 2 (EC 3.6.3.8) (Calcium pump 2) (SERCA2) (SR

Ca(2+)-ATPase 2) (Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle

Isoform) (Endoplasmic reticulum class 1/2 Ca(2+) ATPase). ATA3JHUMAN Sarcoplasmic/endoplasmic reticulum calcium ATPase 3 (EC 3.6.3.8) (Calcium pump 3) (SERCA3) (SR

Ca(2+)-ATPase 3). [Homo sapiens] ATA3_MOUSE Sarcoplasmic/endoplasmic reticulum calcium ATPase 3 (EC 3.6.3.8) (Calcium pump 3) (SERCA3) (SR

Ca(2+)-ATPase 3). [Mus musculus] ATA3_RAT Sarcoplasmic/endoplasmic reticulum calcium ATPase 3 (EC 3.6.3.8) (Calcium pump 3) (SERCA3) (SR

Ca(2+)-ATPase 3). [Rattus norvegicus] ATB1_HUMAN Plasma membrane calcium-transporting ATPase 1 (EC 3.6.3.8) (PMCA1) (Plasma membrane calcium pump isoform 1) (Plasma membrane calcium ATPase Isoform 1). [Homo sapiens] ATB2JHUMAN Plasma membrane calcium-transporting ATPase 2 (EC 3.6.3.8) (PMCA2) (Plasma membrane calcium pump isoform 2) (Plasma membrane calcium ATPase isoform 2). [Homo sapiens] ATB4JHUMAN Plasma membrane calcium-transporting ATPase 4 (EC 3.6.3.8) (PMCA4) (Plasma membrane calcium pump isoform 4) (Plasma membrane calcium ATPase isoform 4). [Homo sapiens] ATCIJHUMAN Calcium-transporting ATPase type 2C, member 1 (EC 3.6.3.8) (ATPase 2C1) (ATP-dependent Ca2+ pump

PMR1) (HUSSY-28). [Homo sapiens] ATHL_HUMAN Potassium-transporting ATPase alpha chain 2 (EC 3.6.3.10) (Proton pump) (Non-gastric H+/K+ ATPase alpha subunit). [Homo sapiens] ATPB_BOVIN ATP synthase beta chain, mitochondria] precursor (EC 3.6.3.14). [Bos taurus]

ATPB_HUMAN ATP synthase beta chain, mitochondrial precursor (EC 3.6.3.14). [Homo sapiens]

ATPB_RAT ATP synthase beta chain, mitochondrial precursor (EC 3.6.3.14). [Rattus norvegicus]

CFTRJHUMAN Cystic fibrosis transmembrane conductance regulator (CFTR) (cAMP- dependent chloride channel). [Homo sapiens] CHD5JHUMAN Chromodomain-helicase-DNA-binding protein 5 (CHD-5). [Homo sapiens] DD15_HUMAN Putative pre-mRNA splicing factor RNA helicase (DEAH box protei DD18_HUMAN ATP-dependent RNA helicase DDX18 (DEAD-box protein 18) (Myc-regulated DEAD-box protein) (MrDb).

[Homo sapiens] DD21_HUMAN Nucleolar RNA helicase II (Nucleolar RNA helicase Gu) (RH II/Gu) (DEAD-box protein 21). [Homo sapiens] DD24JHUMAN ATP-dependent RNA helicase DDX24 (DEAD-box protein 24). [Homo sapiens] DD35JHUMAN Probable ATP-dependent helicase DHX35 (DEAH-box protein 35). [Homo sapiens] DDX1_HUMAN ATP-dependent helicase DDXl (DEAD-box protein 1) (DEAD-box protein- retinoblastoma) (DBP-RB). [Homo sapiens] DDX4_MOUSE DEAD-box protein 4 (VASA homolog) (Mvh). [Mus musculus]

DDX5_HUMAN Probable RNA-dependent helicase p68 (DEAD-box protein p68) (DEAD-box protein 5). [Homo sapiens] DDX7_HUMAN ATP-dependent helicase DDX7 (DEAD-box protein 7) (NP-52). [Homo sapiens] G3BPJHUMAN Ras-GTPase-activating protein binding protein 1 (GAP SH3-domaln binding protein 1) (G3BP-1). [Homo sapiens] HE47JHUMAN Probable ATP-dependent RNA helicase p47 (HLA-B associated transcript- 1). [Homo sapiens] IF41_HUMAN Eukaryotic initiation factor 4A-I (eIF4A-I) (eIF-4A-I). [Homo sapiens] K052_HUMAN Protein KIAA0052 (Fragment). [Homo sapiens] KF1B_HUMAN Kinesin-llke protein KIF1B (Kip). [Homo sapiens]

M10L_HUMAN Moloney leukemia virus 10-like protein 1 (MOVlO-like 1). [Homo sapiens] MCM5 HUMAN DNA replication licensing factor MCM5 (CDC46 homolog) (P1-CDC46). MCM6_HUMAN DNA replication licensing factor MCM6 (P105MCM). [Homo sapiens]

MCM6_RAT DNA replication licensing factor MCM6 (Intestinal DNA replication protein) (Fragment). [Rattus norvegicus]

MCM7JHUMAN DNA replication licensing factor MCM7 (CDC47 homolog) (P1.1-MCM3). [Homo sapiens] MCM8_HUMAN DNA replication licensing factor MCM8 (Minichromosome maintenance 8). [Homo sapiens] MDR1_HUMAN Multidrug resistance protein 1 (P-glycoprotein 1) (CD243 antigen). [Homo sapiens] MRP2_RAT Canalicular multispecific organic anion transporter 1 (Multidrug resistance-associated protein 2)

(Canalicular multidrug resistance protein). [Rattus norvegicus] MRP3_HUMAN Canalicular multispecific organic anion transporter 2 (Multidrug resistance-associated protein 3) (Multi- specific organic anion tranporter-D) (MOAT-D). [Homo sapiens] MRP4JHUMAN Multidrug resistance-associated protein 4 (MRP/cMOAT-related ABC transporter) (Multi-specific organic anion tranporter-B) (MOAT-B). [Homo sapiens] PIA1_HUMAN Protein inhibitor of activated STAT protein 1 (Gu binding protein) (GBP) (RNA helicase II binding protein)

(DEAD/H box-binding protein 1). [Homo sapiens] PR16_HUMAN Pre-mRNA splicing factor ATP-dependent RNA helicase PRP16 (ATP- dependent RNA helicase DHX38)

(DEAH-box protein 38). [Homo sapiens] PRS4JHUMAN 26S protease regulatory subunit 4 (P26s4). [Homo sapiens] PRS6_HUMAN 26S protease regulatory subunit 6B (MIP224) (MB67 interacting protein) (TAT-binding proteiπ-7) (TBP-7).

[Homo sapiens] PRSXJHUMAN 26S protease regulatory subunit S10B (Proteasome subunit p42) (p44) (Conserved ATPase domain protein

44) (CADp₄ ). [Homo sapiens] R51C_HUMAN DNA repair protein RAD51 homolog 3. [Homo sapiens] SKIW_HUMAN Helicase SKI2W (Helicase-like protein) (HLP). [Homo sapiens] U520_HUMAN U5 small nuclear ribonucleoprotein 200 kDa helicase (EC 3.6.1.-) (U5 snRNP-specific 200 kDa protein) (U5-

200KD) (Fragment). [Homo sapiens] VAAIJHUMAN Vacuolar ATP synthase catalytic subunit A, ubiquitous isoform (EC 3.6.3.14) (V-ATPase A subunit 1)

(Vacuolar proton pump alpha subunit 1) (V-ATPase 69 kDa subunit 1) (Isoform VA68). [Homo sapiens] VAB1_HUMAN Vacuolar ATP synthase subunit B, kidney isoform (EC 3.6.3,14) (V- ATPase BI subunit) (Vacuolar proton pump B isoform 1) (Endomembrane proton pump 58 kDa subunit). [Homo sapiens] VATH_HUMAN Vacuolar ATP synthase subunit H (EC 3.6.3.14) (V-ATPase H subunit) (Vacuolar proton pump H subunit)

(V-ATPase 50/57 kDa subunits) (Vacuolar proton pump subunit SFD) (CGI-11). [Homo sapiens]

GTPases

80DP_HUMAN 7,8-dihydro-8-oxoguanine trlphosphatase (EC 3.1.6.-) (8-oxo-dGTPase). [Homo sapiens]

DYN2_HUMAN Dynamin 2 (EC 3.6.1.50). [Homo sapiens]

EF11_HUMAN Elongation factor 1-alpha 1 (EF-l-alpha-1) (Elongation factor 1 A-l) (eEFlA-1) (Elongation factor Tu) (EF-

Tu). [Homo sapiens] EF11_M0USE Elongation factor 1-alpha 1 (EF- -alpha-1) (Elongation factor 1 A-l) (eEFlA-1) (Elongation factor Tu) (EF-

Tu). [Mus musculus] EF12_HUMAN Elongation factor 1-alpha 2 (EF- -alpha-2) (Elongation factor 1 A-2) (eEFlA-2) (Statin SI). [Homo saplens] EFTU_HUMAN Elongation factor Tu, mitochondrial precursor (EF-Tu) (P43). [Homo sapiens] GB02_HUMAN Guanine nucleotlde-binding protein G(θ), alpha subunit 2. [Homo sapiens] GBB1_HUMAN Guanine nucleotlde-binding protein G(I)/G(S)/G(T) beta subunit 1 (Transducin beta chain 1). [Homo sapiens] GBGBJHUMAN Guanine nucleotlde-binding protein G(I)/G(S)/G(θ) gamma-11 subunit. [Homo sapiens] GSP1JHUMAN Gl to S phase transition protein 1 homolog (GTP-blndlπg protein GST1-HS). [Homo sapiens] GTB1_HUMAN GTP-binding protein 1 (G-protein 1) (GP-1) (GP1). [Homo sapiens] IF2PJHUMAN Translation initiation factor IF-2. [Homo sapiens] IF5_HUMAN Eukaryotic translation initiation factor 5 (eIF-5). [Homo sapiens]

NCF1JHUMAN Neutrophil cytosol factor 1 (NCF-1) (Neutrophil NADPH oxidase factor 1) (47 kDa neutrophil oxidase factor)

(p47-phox) (NCF-47K) (47 kDa autosomal chronic granulomatous disease protein). [Homo sapiens] NGP1_HUMAN Autoantlgen NGP-1. [Homo sapiens]

0PA1_HUMAN Dynamin-like 120 kDa protein, mitochondrial precursor (Optic atrophy 1 gene protein). [Homo sapiens] R11AJHUMAN Ras-related protein Rab-llA (Rab-11) (24KG) (YL8). [Homo sapiens] R27B_HUMAN Ras-related protein Rab-27B (C25KG). [Homo sapiens] R33BJHUMAN Ras-related protein Rab-33B. [Homo sapiens]

R39A_HUMAN Ras-related protein Rab-39A (Rab-39). [Homo sapiens]

R39BJHUMAN Ras-related protein Rab-39B. [Homo sapiens]

RAB7JHUMAN Ras-related protein Rab-7. [Homo sapiens]

RAB7_MOUSE Ras-related protein Rab-7. [Mus musculus]

RAC1_HUMAN Ras-related C3 botulinum toxin substrate 1 (p21-Racl) (Ras-like protein TC25). [Homo sapiens]

RAC2_HUMAN Ras-related C3 botulinum toxin substrate 2 (p21-Rac2) (Small G protein) (GX). [Homo sapiens]

RALA_HUMAN Ras-related protein Ral-A. [Homo sapiens]

RAN_HUMAN GTP-binding nuclear protein RAN (TC4) (Ran GTPase) (Androgen receptor- associated protein 24). [Homo sapiens]

RAPAJHUMAN Ras-related protein Rap-IA (C21KG) (KREV-l protein) (GTP-binding protein SMG-P21A) (G-22K). [Homo sapiens]

RASH_HUMAN Transforming protein p21/H-Ras-l (c-H-ras). [Homo sapiens]

RB14JHUMAN Ras-related protein Rab-14. [Homo sapiens]

RB1A_HUMAN Ras-related protein Rab-IA (YPTl-related protein). [Homo sapiens]

RB20JHUMAN Ras-related protein Rab-20. [Homo sapiens]

RB4BJHUMAN Ras-related protein Rab-4B. [Homo sapiens]

RB5AJHUMAN Ras-related protein Rab-5A. [Homo sapiens]

RB6A_HUMAN Ras-related protein Rab-6A (Rab-6). [Homo sapiens]

RGSBJ-IUMAN Regulator of G-protein signaling 11 (RGS11). [Homo sapiens]

RHOG_HUMAN Rho-related GTP-binding protein RhoG (Sidl0750). [Homo sapiens]

RHON_HUMAN Rho-related GTP-binding protein RhoN (Rho7) (Rnd2). [Homo sapiens]

SADIJHUMAN SAM domain and HD domain-containing protein 1 (Dendritic cell-derived IFNG-induced protein) (DCIP)

(Monocyte protein 5) (MOP-5). [Homo sapiens]

Other ATP binding proteins

ACLYJHUMAN ATP-citrate synthase (EC 2.3.3.8) (ATP-cltrate (pro-S-)-lyase) (Citrate cleavage enzyme). [Homo sapiens] ACLY_RAT ATP-cltrate synthase (EC 2.3.3.8) (ATP-citrate (pro-S-)-lyase) (Citrate cleavage enzyme). [Rattus norvegicus] ASSY_HUMAN Arglninosucclnate synthase (EC 6.3.4.5) (Citrulline— aspartate

ASSY_MOUSE Argininosuccinate synthase (EC 6.3.4.5) (Citrulline— aspartate ligase). [Mus musculus] ASSY_RAT Argininosuccinate synthase (EC 6.3.4.5) (Citrulllne—aspartate ligase). [Rattus norvegicus]

ATPA_HUMAN ATP synthase alpha chain, mitochondrial precursor (EC 3.6.3.14). [Homo sapiens] C1TCJ-IUMAN C-1-tetrahydrofolate synthase, cytoplasmic (Cl-THF synthase) [Includes: Methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5); Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9); Formyltetrahydrofolate synthetase (EC 6.3.4.3)]. [Homo sapiens] C2TA_HUMAN MHC class II transactivator (CIITA). [Homo sapiens] CCAB_HUMAN Voltage-dependent N-type calcium channel alpha-IB subunit (Calcium channel, L type, alpha-1 polypeptide

Isoform 5) (Brain calcium channel III) (Bill). [Homo sapiens] CH60_CRIGR 60 kDa heat shock protein, mitochondrial precursor (Hsp60) (60 CH60JHUMAN 60 kDa heat shock protein, mitochondrial precursor (HspδO) (60 CH60_MOUSE 60 kDa heat shock protein, mitochondrial precursor (Hsp60) (60 kDa chaperonin) (CPN60) (Heat shock protein 60) (HSP-60) (Mitochondrial matrix protein PI) (HSP-65). [Mus musculus] C0A1_HUMAN Acetyl-CoA carboxylase 1 (EC 6.4.1.2) (ACC-alpha) [Includes: Biotin carboxylase (EC 6.3.4.14)]. [Homo sapiens] CPSMJ-IUMAN Carbamoyl-phosphate synthase [ammonia], mitochondrial precursor (EC 6.3.4.16) (Carbamoyl-phosphate synthetase I) (CPSase I). [Homo sapiens] CPSM_RAT Carbamoyl-phosphate synthase [ammonia], mitochondrial precursor (EC 6.3.4.16) (Carbamoyl-phosphate synthetase I) (CPSASE I). [Rattus norvegicus] DN2LJHUMAN DNA2-like homolog (DNA replication helicase-like homolog) (Fragment). [Homo sapiens] DNL1J-IUMAN DNA ligase I (EC 6.5.1.1) (Polydeoxyribonucleotide synthase [ATP]). [Homo sapiens] DYH9_HUMAN Ciliary dynein heavy chain 9 (Axonemal beta dynein heavy chain 9). [Homo sapiens] DYHBJHUMAN Ciliary dynein heavy chain 11 (Axonemal beta dynein heavy chain 11). [Homo sapiens] DYHC_HUMAN Dynein heavy chain, cytosolic (DYHC) (Cytoplasmic dynein heavy chain 1) (DHC1) (Fragment). [Homo sapiens] EHD3_HUMAN EH-domaln containing protein 3. [Homo sapiens] EHD3_MOUSE EH-domain containing protein 3. [Mus musculus]

EHD4_HUMAN EH-domain containing protein 4 (EH domain-containing protein FKSG7) (Hepatocellular carcinoma- associated protein 10/11). [Homo sapiens] ENPL_CANFA Endoplasmin precursor (94 kDa glucose-regulated protein) (GRP94). [Canls familiaris] ENPL_HUMAN Endoplasmin precursor (94 kDa glucose-regulated protein) (GRP94) (gp96 homolog) (Tumor rejection antigen 1). [Homo sapiens] ENPL_MOUSE Endoplasmin precursor (Endoplasmic reticulum protein 99) (94 kDa glucose-regulated protein) (GRP94)

(ERP99) (Polymorphic tumor rejection antigen 1) (Tumor rejection antigen gp96). [Mus musculus] FOLCJHUMAN Folylpolyglutamate synthase, mitochondrial precursor (EC 6.3.2.17) (Folylpoly-gamma-glutamate synthetase) (FPGS). [Homo sapiens] GEF2JHUMAN Ganglloside expression factor 2 (GEF-2) (General protein transport factor pl6) (GATE-16) (GABA(A) receptor-associated protein-like 2) (MAPI light chain 3 related protein). [Homo sapiens] GR75_MOUSE Stress-70 protein, mitochondrial precursor (75 kDa glucose regulated protein) (GRP 75) (Peptide-binding protein 74) (PBP74) (P66 MOT) (Mortalin). [Mus musculus] GR78_HUMAN 78 kDa glucose-regulated protein precursor (GRP 78) (Immunoglobulin heavy chain binding protein) (BiP)

(Endoplasmic reticulum lumenal Ca(2+) binding protein grp78). [Homo sapiens] GR78_RAT 78 kDa glucose-regulated protein precursor (GRP 78) (Immunoglobulin heavy chain binding protein) (BIP)

(Steroldogenesis-actlvator polypeptide). [Rattus norvegicus] GUAAJHUMAN GMP synthase [glutamlne-hydrolyziπg] (EC 6.3.5.2) (Glutamine amidotransferase) (GMP synthetase).

[Homo sapiens] HELZ HUMAN Potential helicase with zinc-finger domain. [Homo sapiens] HS71_HUMAN Heat shock 70 kDa protein 1 (HSP70.1) (HSP70-1/HSP70-2). [Homo sapiens] HS72JHUMAN Heat shock-related 70 kDa protein 2 (Heat shock 70 kDa protein 2). [Homo sapiens] HS72_MOUSE Heat shock-related 70 kDa protein 2 (Heat shock protein 70.2). [Mus musculus] HS72_RAT Heat shock-related 70 kDa protein 2 (Heat shock protein 70.2) (Te

HS7C_BOVIN Heat shock cognate 71 kDa protein. [Bos taurus]

HS7C_MOUSE Heat shock cognate 71 kDa protein. [Mus musculus]

HS7HJHUMAN Heat shock 70 kDa protein 1-HOM (HSP70-HOM). [Homo sapiens]

HS9AJHUMAN Heat shock protein HSP 90-alpha (HSP 86). [Homo sapiens]

HS9A_PIG Heat shock protein HSP 90-alpha (HSP 86). [Sus scrofa]

HS9B_MOUSE Heat shock protein HSP 90-beta (HSP 84) (Tumor specific transplantation 84 kDa antigen) (TSTA). [Mus musculus] KF11JHUMAN Klnesin-llke protein KIFll (Kinesin-related motor protein Eg5) (Kinesin-like spindle protein HKSP) (Thyroid receptor interacting protein 5) (TRIP5) (Kinesln-like protein 1). [Homo sapiens] KF14_HUMAN Klnesin-like protein KIF14. [Homo sapiens]

KF1AJHUMAN Kinesln-like protein KIF1A (Axonal transporter of synaptic vesicles). [Homo sapiens] KF23_HUMAN Kinesin-like protein KIF23 (Mitotic klnesin-like protein-1) (Klnesin- like protein 5). [Homo sapiens] KF2C_HUMAN Kinesin-like protein KIF2C (Mitotic centromere-associated kinesin) (MCAK) (Klnesin-like protein 6). [Homo sapiens] KF4AJHUMAN Chromosome-associated kinesin KIF4A (Chromoklnesin). [Homo sapiens] KF5C_HUMAN Kinesin heavy chain isoform 5C (Kinesin heavy chain neuron-specific 2). [Homo sapiens] KG88_HUMAN Protein KIAA1688. [Homo sapiens] KI67_HUMAN Antigen KI-67. [Homo sapiens] KIF9_HUMAN Kinesin-like protein KIF9. [Homo sapiens]

KINH_HUMAN Kinesin heavy chain (Ubiquitous kinesin heavy chain) (UKHC). [Homo sapiens] MCCAJHUMAN Methylcrotonyl-CoA carboxylase alpha chain, mitochondrial precursor (EC 6.4.1.4) (3-Methylcrotonyl-CoA carboxylase 1) (MCCase alpha subunit) (3-methylcrotonyl-CoA:carbon dioxide ligase alpha subunit). [Homo sapiens] METK_HUMAN S-adenosylmethionine synthetase gamma form (EC 2.5.1.6) (Methionine adenosyltransferase) (AdoMet synthetase) (MAT-II). [Homo sapiens] METK_RAT S-adenosyl methionine synthetase gamma form (EC 2.5.1.6) (Methionine adenosyltransferase) (AdoMet synthetase) (MAT-II). [Rattus norvegicus] METL_HUMAN S-adenosylmethlonine synthetase alpha and beta forms (EC 2.5.1.6) (Methionine adenosyltransferase)

(AdoMet synthetase) (MAT-I/III). [Homo sapiens] MSH4_HUMAN MutS protein homolog 4. [Homo sapiens] MY15_HUMAN Myosin XV (Unconventional myosln-15). [Homo sapiens] MY1B_M0USE Myosin lb (Myosin I alpha) (MMI-alpha) (MMIa) (MIH-L). [Mus musculus] MY1C_HUMAN Myosin Ic (Myosin I beta) (MMI-beta) (MMIb). [Homo sapiens] MY5C_HUMAN Myosin Vc (Myosin 5C). [Homo sapiens] MY7A_HUMAN Myosin Vila. [Homo sapiens]

MY9B_HUMAN Myosin IXb (Unconventional myosin-9b). [Homo sapiens]

MYH1JHUMAN Myosin heavy chain, skeletal muscle, adult 1 (Myosin heavy chain Ilx/d) (MyHC-IIx/d). [Homo sapiens] MYH3_HUMAN Myosin heavy chain, fast skeletal muscle, embryonic (Muscle embryonic myosin heavy chain) (SMHCE).

[Homo sapiens] MYH6JHUMAN Myosin heavy chain, cardiac muscle alpha Isoform (MyHC-alpha). [Homo sapiens] MYH6_MOUSE Myosin heavy chain, cardiac muscle alpha isoform (MyHC-alpha). [Mus musculus] MYH7_HUMAN Myosin heavy chain, cardiac muscle beta isoform (MyHC-beta). [Homo sapiens] MYH7_RAT Myosin heavy chain, cardiac muscle beta isoform (MyHC-beta). [Rattus norvegicus]

MYH9JUMAN Myosin heavy chain, nonmuscle type A (Cellular myosin heavy chain, type A) (Nonmuscle myosin heavy chain-A) (NMMHC-A). [Homo sapiens] MYH9_RAT Myosin heavy chain, nonmuscle type A (Cellular myosin heavy chain, type A) (Nonmuscle myosin heavy chain-A) (NMMHC-A). [Rattus norvegicus] MYHAJHUMAN Myosin heavy chain, nonmuscle type B (Cellular myosin heavy chain, type B) (Nonmuscle myosin heavy chain-B) (NMMHC-B). [Homo sapiens] NAL1_HUMAN NACHT-, LRR- and PYD-contalning protein 2 (Death effector filament- forming ced-4-like apoptosis protein)

(Nucleotlde-binding domain and caspase recruitment domain) (Caspase recruitment domain protein 7).

[Homo sapiens] NP14JHUMAN Nucleolar phosphoproteln pl30 (Nucleolar 130 kDa protein) (140 kDa nucleolar phosphoproteln) (Noppl40)

(Nucleolar and coiled-body phosphoproteln 1). [Homo sapiens] NSFJHUMAN Vesicle-fusing ATPase (EC 3.6.4.6) (Vesicular-fusion protein NSF) (N- ethylmaleimide sensitive fusion protein) (NEM-sensitive fusion protein). [Homo sapiens] NUDM_HUMAN NADH-ubiquinone oxidoreductase 42 kDa subunit, mitochondria] precursor (EC 1.6.5.3) (EC 1.6.99.3)

(Complex I-42KD) (CI-42KD). [Homo sapiens] OASL_HUMAN 59 kDa 2'-5'-oligoadenylate synthetase like protein (p59 OASL) (p590ASL) (Thyroid receptor interacting protein 14) (TRIP14). [Homo sapiens] OXRP HUMAN 150 kDa oxygen-regulated protein precursor (Orpl50) (Hypoxia up- regulated 1). [Homo sapiens] P2X1_RAT P2X purinoceptor 1 (ATP receptor) (P2X1) (Purlnerglc receptor) (RP-2 protein). [Rattus norvegicus]

PCCAJHUMAN Propionyl-CoA carboxylase alpha chain, mitochondrial precursor PEBP_BOVIN Phosphatidylethanolamine-binding protein (PEBP) (HCNPpp) (Basic cytosolic 21 kDa protein) [Contains:

Hlppocampal cholinergic neurostlmulatlng peptide (HCNP)]. [Bos taurus] PEBP_MACFA Phosphatidylethanolamlne-binding protein (PEBP) (HCNPpp) [Contains: Hlppocampal cholinergic neurostlmulatlng peptide (HCNP)]. [Macaca fascicularls] PEBP_MOUSE Phosphatidylethanolamine-binding protein (PEBP). [Mus musculus] PEBP_RAT Phosphatldylethanolamine-binding protein (PEBP) (Hlppocampal chol

PMS2_HUMAN PMS1 protein homolog 2 (DNA mismatch repair protein PMS2). [Homo sapiens] PRS7JHUMAN 26S protease regulatory subunit 7 (MSS1 protein). [Homo sapiens] PRS7_MOUSE 26S protease regulatory subunit 7 (MSS1 protein). [Mus musculus] PRS7_RAT 26S protease regulatory subunit 7 (MSS1 protein). [Rattus norvegi

PRSA_MOUSE 26S protease regulatory subunit 6A (TAT-binding protein 1) (TBP-1). [Mus musculus] PRSA_RAT 26S protease regulatory subunit 6A (TAT-binding protein 1) (TBP-1) (Spermatogenic cell/sperm-associated

TAT-blnding protein homolog SATA). [Rattus norvegicus] PUR4_HUMAN Phosphoribosylformylglycinamldine synthase (EC 6.3.5.3) (FGAM synthase) (FGAMS) (Formylglycinamide ribotlde amidotransferase) (FGARAT) (Formylglycinamide ribotide synthetase). [Homo sapiens] PYC_HUMAN Pyruvate carboxylase, mitochondrial precursor (EC 6.4.1.1) (Pyruvic carboxylase) (PCB). [Homo sapiens] PYC_MOUSE Pyruvate carboxylase, mitochondrial precursor (EC 6.4.1.1) (Pyruvic carboxylase) (PCB). [Mus musculus] PYC_RAT Pyruvate carboxylase, mitochondrial precursor (EC 6.4.1.1) (Pyruvic carboxylase) (PCB). [Rattus norvegicus] PYR1_HUMAN CAD protein [Includes: Glutamlne-dependent carbamoyl-phosphate synthase (EC 6.3.5.5); Aspartate carbamoyltransferase (EC 2.1.3.2); Dihydroorotase (EC 3.5.2.3)]. [Homo sapiens] Q63861 Smooth muscle myosin heavy chain isoform SM1A (Fragment). [Rattus norvegicus]

Q8IUN3 Similar to klnesin-like protein at 64D (Fragment). [Homo sapiens]

RNT1_HUMAN Regulator of nonsense transcripts 1 (Nonsense mRNA reducing factor 1) (NORF1) (Up-frameshlft suppressor 1 homolog). [Homo sapiens] RNTl_MOUSE Regulator of nonsense transcripts 1 (Nonsense mRNA reducing factor 1) (NORF1) (Up-frameshift suppressor 1 homolog). [Mus musculus] ROUJHUMAN Heterogenous nuclear ribonucleoprotein U (hnRNP U) (Scaffold attachment factor A) (SAF-A). [Homo sapiens] RUVIJHUMAN RuvB-like 1 (EC 3.6.1.-) (49-kDa TATA box-binding protein-Interacting protein) (49 kDa TBP-interacting protein) (TIP49a) (Pontln 52) (Nuclear matrix protein 238) (NMP 238) (54 kDa erythrocyte cytosolic protein) (ECP-54) (TIP60-associated protein 54-alpha) STCH_HUMAN Microsomal stress 70 protein ATPase core precursor. [Homo sapiens] SYAJHUMAN Alanyl-tRNA synthetase (EC 6.1.1.7) (Alanine-tRNA ligase) (AlaRS). [Homo sapiens] SYD_HUMAN Aspartyl-tRNA synthetase (EC 6.1.1.12) (Aspartate-tRNA ligase) (AspRS). [Homo sapiens] SYEPJHUMAN Bifunctional aminoacyl-tRNA synthetase [Includes: Glutamyl-tRNA synthetase (EC 6.1.1.17) (Glutamate- tRNA ligase); Prolyl-tRNA synthetase (EC 6.1.1.15) (Prollne-tRNA ligase)]. [Homo sapiens] SYFA_HUMAN Phenylaianyl-tRNA synthetase alpha chain (EC 6.1.1.20) (Phenylalanine- -tRNA ligase alpha chain) (PheRS)

(CML33). [Homo sapiens] SYFB_HUMAN Phenylaianyl-tRNA synthetase beta chain (EC 6.1.1.20) (Phenylalanine- tRNA ligase beta chain) (PheRS)

(HSPC173). [Homo sapiens] SYGJHUMAN Giycyl-tRNA synthetase (EC 6.1.1.14) (Glycine-tRNA ligase) (GlyRS). [Homo sapiens] SYG_MOUSE Glycyl-tRNA synthetase (EC 6.1.1.14) (Glyclne-tRNA ligase) (GlyRS). [Mus musculus] SYH_HUMAN Histidyl-tRNA synthetase (EC 6.1.1.21) (Histidine-tRNA ligase) (HisRS). [Homo sapiens] SYI_HUMAN Isoleucyl-tRNA synthetase, cytoplasmic (EC 6.1.1.5) (Isoieucine-tRNA ligase) (IleRS) (IRS). [Homo sapiens] SYK_HUMAN Lysyl-tRNA synthetase (EC 6.1.1.6) (Lysine-tRNA ligase) (LysRS). [Homo sapiens]

SYLMJHUMAN Probable leucyl-tRNA synthetase, mitochondrial precursor (EC 6.1.1.4) (Leucine— tRNA ligase) (LeuRS).

[Homo sapiens] SYN HUMAN Asparaginyl-tRNA synthetase, cytoplasmic (EC 6.1.1.22) (Asparagl

SYQ_HUMAN Glutaminyl-tRNA synthetase (EC 6.1.1.18) (Glutamine-tRNA ligase) (GlnRS). [Homo sapiens] SYRJHUMAN Arginyl-tRNA synthetase (EC 6.1.1.19) (Arginine-tRNA ligase) (ArgRS). [Homo sapiens] SYR_MOUSE Arginyl-tRNA synthetase (EC 6.1.1.19) (Arginine— tRNA ligase) (ArgRS). [Mus musculus] SYV2JHUMAN Valyl-tRNA synthetase 2 (EC 6.1.1.9) (Valine-tRNA ligase 2) (ValRS 2) (G7a). [Homo sapiens] SYV_RAT Valyl-tRNA synthetase (EC 6.1.1.9) (Valine-tRNA ligase) (ValRS) (Fragment). [Rattus norvegicus]

SYWM_HUMAN Tryptophanyl-tRNA synthetase, mitochondrial precursor (EC 6.1.1.2) (Tryptophan—tRNA ligase) (TrpRS)

((Mt)TrpRS). [Homo sapiens] SYWM_MOUSE Tryptophanyl-tRNA synthetase, mitochondrial precursor (EC 6.1.1.2) (Tryptophan-tRNA ligase) (TrpRS)

((Mt)TrpRS). [Mus musculus] SYW_HUMAN Tryptophanyl-tRNA synthetase (EC 6.1.1.2) (Tryptophan-tRNA liga

SYW_MOUSE Tryptophanyl-tRNA synthetase (EC 6.1.1.2) (Tryptophan-tRNA ligase) (TrpRS). [Mus musculus] SYY_HUMAN Tyrosyl-tRNA synthetase (EC 6.1.1.1) (Tyrosyl-tRNA ligase) (TyrRS). [Homo sapiens]

TCPAJHUMAN T-complex protein 1, alpha subunit (TCP-1-alpha) (CCT-alpha). [Homo sapiens] TCPDJHUMAN T-complex protein 1, delta subunit (TCP-1-delta) (CCT-delta) (Stimulator of TAR RNA binding). [Homo sapiens] TCPD_MOUSE T-complex protein 1, delta subunit (TCP-1-delta) (CCT-delta) (A45). [Mus musculus] TCPE_MOUSE T-complex protein 1, epsllon subunit (TCP-1-epsllon) (CCT-epsilon). [Mus musculus] TCPGJHUMAN T-complex protein 1, gamma subunit (TCP-1-gamma) (CCT-gamma).

TCPH_HUMAN T-complex protein 1, eta subunit (TCP-1-eta) (CCT-eta) (HIV-1 Nef interacting protein). [Homo sapiens] TCPQJHUMAN T-complex protein 1, theta subunit (TCP-1-theta) (CCT-theta). [Homo sapiens] TCPWJHUMAN T-complex protein 1, zeta-2 subunit (TCP-l-zeta-2) (CCT-zeta-2) (TCP- 1-zeta-like) (CCT-zeta-like)

(Testis-specific Tcp20) (Testis-specific protein TSA303). [Homo sapiens] TCPZJHUMAN T-complex protein 1, zeta subunit (TCP-1-zeta) (CCT-zeta) (CCT-zeta-1) (Tcp20) (HTR3). [Homo sapiens] TERA_HUMAN Transitional endoplasmic reticulum ATPase (TER ATPase) (15S Mg(2+)- ATPase p97 subunit) (Valosin containing protein) (VCP) [Contains: Valosin]. [Homo sapiens] TERA_MOUSE Transitional endoplasmic reticulum ATPase (TER ATPase) (15S Mg(2+)- ATPase p97 subunit) (Valosin containing protein) (VCP) [Contains: Valosin]. [Mus musculus] TERA_PIG Transitional endoplasmic reticulum ATPase (TER ATPase) (15S Mg(2+)- ATPase p97 subunit) (Valosin containing protein) (VCP) [Contains: Valosin (Peptide VQY)]. [Sus scrofa] TERA_RAT Transitional endoplasmic reticulum ATPase (TER ATPase) (15S Mg(2+)- ATPase p97 subunit) (Valosin containing protein) (VCP) [Contains: Valosin]. [Rattus norvegicus] TP2A_HUMAN DNA topoisomerase II, alpha isozyme (EC 5.99.1.3). [Homo sapiens] TP2B_HUMAN DNA topoisomerase II, beta Isozyme (EC 5.99.1.3). [Homo sapiens] TRAL_HUMAN Heat shock protein 75 kDa, mitochondrial precursor (HSP 75) (Tumor necrosis factor type 1 receptor associated protein) (TRAP-1) (TNFR- associated protein 1). [Homo sapiens] TRAL_MOUSE Heat shock protein 75 kDa, mitochondrial precursor (HSP 75) (Tumor necrosis factor type 1 receptor associated protein) (TRAP-1) (TNFR- associated protein 1). [Mus musculus]

Transmembrane receptors

5H1F_RAT 5-hydroxytryptamine IF receptor (5-HT-1F) (Serotonin receptor). [Rattus norvegicus]

ACHEJHUMAN Acetylchollne receptor protein, epsilon chain precursor. [Homo sapiens]

AG2S_HUMAN Type-IB anglotensin II receptor (AT1B) (AT1BR). [Homo sapiens]

AMRPJHUMAN Alpha-2-macroglobulin receptor-associated protein precursor (Alpha-2-MRAP) (Low density lipoprotein receptor-related protein- associated protein 1) (RAP). [Homo sapiens] B2MG_HUMAN Beta-2-microglobulin precursor (HDCMA22P). [Homo sapiens] CD45_HUMAN Leukocyte common antigen precursor (EC 3.1.3.48) (L-CA) (CD45 antigen) (T200). [Homo sapiens] CD4_HUMAN T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3). [Homo sapiens] CKR4JHUMAN C-C chemokine receptor type 4 (C-C CKR-4) (CC-CKR-4) (CCR-4) (CCR4) (K5-5). [Homo sapiens] CRCP_HUMAN Calcitonin gene-related peptlde-receptor component protein (CGRP- receptor component protein) (CGRP-

RCP) (CGRPRCP). [Homo sapiens] DAG1_HUMAN Dystroglycan precursor (Dystrophin-associated glycoprotein 1) [Contains: Alpha-dystroglycan (Alpha-DG);

Beta-dystroglycan (Beta- DG)]. [Homo sapiens] DBDR_HUMAN D(1B) dopamine receptor (D(5) dopamine receptor) (Dlbeta dopamine receptor). [Homo sapiens] ENTK_HUMAN Enteropeptldase precursor (EC 3.4.21.9) (Enteroklnase). [Homo sapiens] FZD6_HUMAN Frizzled 6 precursor (Frizzled-6) (Fz-6) (hFz6). [Homo sapiens]

GAA6_HUMAN Gamma-aminobutyric-acid receptor alpha-6 subunit precursor (GABA(A) receptor). [Homo sapiens] GADJHUMAN Gamma-aminobutyric-acid receptor delta subunit precursor (GABA(A) receptor). [Homo sapiens] GAE_HUMAN Gamma-aminobutyric-acid receptor epsilon subunit precursor (GABA(A) receptor). [Homo sapiens] GLK1JHUMAN Glutamate receptor, ionotropic kainate 1 precursor (Glutamate receptor 5) (GluR-5) (GluR5) (Excitatory amino acid receptor 3) (EAA3). [Homo sapiens] GLK2_HUMAN Glutamate receptor, ionotropic kainate 2 precursor (Glutamate receptor 6) (GluR-6) (GluR6) (Excitatory amino acid receptor 4) (EAA4). [Homo sapiens] GLK3_HUMAN Glutamate receptor, ionotropic kainate 3 precursor (Glutamate receptor 7) (GluR-7) (GluR7) (Excitatory amino acid receptor 5) (EAA5). [Homo sapiens] GP35_HUMAN Probable G protein-coupled receptor GPR35. [Homo sapiens] GP61_HUMAN Probable G protein-coupled receptor GPR61 (Blogenlc amine receptor- like G-protein-coupled receptor).

[Homo sapiens] GPBA_HUMAN Platelet glycoprotein lb alpha chain precursor (GP-Ib alpha) (G

HB2BJHUMAN HLA class II hlstocompatibility antigen, DR-1 beta chain precursor (Clone P2-beta-3). [Homo sapiens] I12S_HUMAN Interleukin-12 receptor beta-2 chain precursor (IL-12 receptor beta- 2) (IL-12R-beta2). [Homo sapiens] INGR_HUMAN Interferon-gamma receptor alpha chain precursor (CDwll9). [Homo sapiens] INGR_MOUSE Interferon-gamma receptor alpha chain precursor. [Mus musculus] K2S1_HUMAN Killer cell immunoglobulin-iike receptor 2DS1 precursor (MHC class I NK cell receptor Eb6 Actl). [Homo sapiens] LDVR_HUMAN Very low-density lipoprotein receptor precursor (VLDL receptor). LEPR_RAT Leptln receptor precursor (LEP-R) (OB receptor) (OB-R). [Rattus norvegicus]

LGR5_HUMAN Leucine-rich repeat-containing G protein-coupled receptor 5 precursor (Orphan G protein-coupled receptor

HG38) (G protein-coupled receptor 49). [Homo sapiens] LGR8_HUMAN Relaxin receptor 2 (Leucine-rich repeat-containing G protein-coupled receptor 8) (G protein-coupled receptor affecting testicular descent). [Homo sapiens] MGR1JHUMAN Metabotropic glutamate receptor 1 precursor (mGluRl). [Homo sapiens] MGR5_HUMAN Metabotropic glutamate receptor 5 precursor (mGluRS). [Homo sapiens] MGR7_HUMAN Metabotropic glutamate receptor 7 precursor (mGluR7). [Homo sapiens] NTR1_RAT Neurotensin receptor type 1 (NT-R-1) (High-affinity levocabastine- Insensitive neurotensln receptor)

(NTRH). [Rattus norvegicus] OPCM_HUMAN Oplold binding protein/cell adhesion molecule precursor (OBCAM) (Opioid-binding cell adhesion molecule)

(OPCML). [Homo sapiens] OPSG_HUMAN Green-sensitive opsin (Green cone photoreceptor pigment). [Homo sapiens] OX2R_HUMAN Orexin receptor type 2 (Ox2r) (Hypocretin receptor type 2). [Homo sapiens] PLX4_HUMAN Plexin A3 precursor (Plexin 4) (Transmembrane protein sex). [Homo sapiens]

PTPK_HUMAN Receptor-type proteln-tyrosine phosphatase kappa precursor (EC 3.1.3.48) (R-PTP-kappa). [Homo sapiens] PTPU_HUMAN Receptor-type protein-tyroslne phosphatase U precursor (EC 3.1.3.48) (R-PTP-U) (Proteln-tyrosine phosphatase J) (PTP-J) (Pancreatic carcinoma phosphatase 2) (PCP-2). [Homo sapiens] PTPXJHUMAN Receptor-type protein-tyrosine phosphatase N2 precursor (EC 3.1.3.48) (R-PTP-N2) (Islet cell autoantigen related protein) (ICAAR) (IAR) (Phogrin). [Homo sapiens] PTPZ_HUMAN Receptor-type protein-tyroslne phosphatase zeta precursor (EC 3.1.3.48) (R-PTP-zeta). [Homo sapiens] Q30120 MHC class II HLA-DR-beta precursor. [Homo sapiens]

RGR_HUMAN RPE-retinal G protein-coupled receptor. [Homo sapiens] ROM_HUMAN Heterogeneous nuclear ribonucleoprotein M (hnRNP M). [Homo sapiens] RRBl_MOUSE Ribosome-binding protein 1 (Ribosome receptor protein) (mRRp). [Mus musculus] RSP4_HUMAN 40S ribosomal protein SA (P40) (34/67 kDa laminin receptor) (Colon carcinoma laminin-binding protein)

(NEM/1CHD4) (Multidrug resistance- associated protein MGrl-Ag). [Homo sapiens] TFR1_HUMAN Transferrin receptor protein 1 (TfRl) (TR) (TfR) (Trfr) (CD71 antigen) (T9) (p90). [Homo sapiens] TLR2_MOUSE Toll-like receptor 2 precursor. [Mus musculus] TLR9JHUMAN Toll-like receptor 9 precursor. [Homo sapiens] TMS2_HUMAN Transmembrane protease, serine 2 precursor (EC 3.4.21.-). [Homo sapiens]

Other nucleotide binding proteins

AFP2_HUMAN Arfaptin 2 (ADP-rlbosylation factor Interacting protein 2) (Partner of RACl) (PORl protein). [Homo sapiens]

CNG1_HUMAN cGMP-gated cation channel alpha 1 (CNG channel alpha 1) (CNG-1)

DEK HUMAN DEK protein. [Homo sapiens]

DPOZ_HUMAN DNA polymerase zeta catalytic subunit (EC 2.7.7.7) (hREV3). [Homo sapiens]

DPOZ_MOUSE DNA polymerase zeta catalytic subunit (EC 2.7.7.7) (Seizure-related protein 4). [Mus musculus]

GBAS_MOUSE Guanine nucleotlde-binding protein G(S), alpha subunit (Adenylate cyclase-stimulatlng G alpha protein).

[Mus musculus] HCN1_RAT Potassium/sodium hyperpolarizatlon-activated cyclic nucleotide-gated channel 1. [Rattus norvegicus]

PTD4_HUMAN Putative GTP-binding protein PTD004 (PR02455). [Homo sapiens] PTD4_MOUSE Putative GTP-binding protein PTD004 homolog. [Mus musculus] Q9GKK5 Gamma tubulin. [Canis famlliaris]

SEP6_HUMAN Septln 6. [Homo sapiens] SRPR--HUMAN Signal recognition particle receptor alpha subunit (SR-alpha) (Docking protein alpha) (DP-alpha). [Homo sapiens] SUCA_HUMAN Succinyl-CoA ligase [GDP-forming] alpha-chain, mitochondrial precursor (EC 6.2.1.4) (Succinyl-CoA synthetase, alpha chain) (SCS-alpha). [Homo sapiens] SUCA_MOUSE Succinyl-CoA ligase [GDP-forming] alpha-chain, mitochondrial precursor (EC 6.2.1.4) (Succinyl-CoA synthetase, alpha chain) (SCS-alpha). [Mus musculus] SUCA_RAT Succinyl-CoA ligase [GDP-forming] alpha-chain, mitochondrial precursor (EC 6.2.1.4) (Succinyl-CoA synthetase, alpha chain) (SCS-alpha). [Rattus norvegicus]

TBA1_HUMAN Tubulin alpha-1 chain (Alpha-tubulin 1). [Homo sapiens]

TBA1_M0USE Tubulin alpha-1 chain. [Mus musculus]

TBA4JHUMAN Tubulin alpha-4 chain (Alpha-tubulln 4). [Homo sapiens]

TBA6_HUMAN Tubulin alpha-6 chain (Alpha-tubulin 6). [Homo sapiens]

TBA8_HUMAN Tubulin alpha-8 chain (Alpha-tubulin 8). [Homo sapiens]

TBA_PIG Tubulin alpha chain. [Sus scrofa]

TBB1_HUMAN Tubulin beta-1 chain. [Homo sapiens]

TBB1_RAT Tubulin beta chain (T beta-15). [Rattus norvegicus]

TBB2_HUMAN Tubulin beta-2 chain. [Homo sapiens]

TBB3_MOUSE Tubulin beta-3. [Mus musculus]

TBB4_MOUSE Tubulin beta-4 chain. [Mus musculus]

TBB5_HUMAN Tubulin beta-5 chain. [Homo sapiens]

TBBQJHUMAN Tubulin beta-4q chain. [Homo sapiens]

TBB_PIG Tubulin beta chain. [Sus scrofa]

TBD_HUMAN Tubulin delta chain (Delta tubulin). [Homo sapiens]

Oxidoreductases, acting on NADH or NADPH

GSHR HUMAN Glutathlone reductase, mitochondrial precursor (EC 1.8.1.7) (GR) (GRase). [Homo sapiens]

GSHR _MOUSE Glutathione reductase, mitochondrial precursor (EC 1.8.1.7) (GR) (GRase). [Mus musculus]

GTOl _HUMAN Glutathlone transferase omega 1 (EC 2.5.1.18) (GSTO 1-1). [Homo sapiens]

NCPR. HUMAN NADPH-cytochrome P450 reductase (EC 1.6.2.4) (CPR) (P450R). [Homo sapiens]

NIA1_ HORVU Nitrate reductase [NADH] (NR)

NU5M HUMAN NADH-ubiquinone oxidoreductase chain 5 (EC 1.6.5.3). [Homo sapiens]

NUAM. _HUMAN NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial precursor (EC 1.6.5.3) (EC 1.6.99.3)

(Complex I-75Kd) (CI-75Kd). [Homo sapiens]

PDX3_ .HUMAN Thioredoxln-dependent peroxide reductase, mitochondrial precursor (EC 1.11.1.-) (Peroxlredoxin 3)

(Antloxidant protein 1) (AOP-1) (MER5 protein homolog) (HBC189) (PRX III). [Homo sapiens]

QOR 1 HUMAN Quinone oxidoreductase (EC 1.6.5.5) (NADPH :quinone reductase) (Zeta- crystallin). [Homo sapiens]

QOR MOUSE Quinone oxidoreductase (EC 1.6.5.5) (NADPH :quinone reductase) (Zeta- crystallin). [Mus musculus]

VAT1 .HUMAN Synaptlc vesicle membrane protein VAT-1 homolog. [Homo sapiens]

Other oxidoreductases

3BH2_RAT 3 beta-hydroxysteroid dehydrogenase/delta 5— >4-isomerase type II (3Beta-HSD II) [Includes: 3-beta- hydroxy-delta(5)-steroid dehydrogenase (EC 1.1.1.145) (3-beta-hydroxy-5-ene steroid dehydrogenase) (Progesterone reductase); Steroid delta-isomerase (EC 5.3

6PGD_SHEEP 6-phosphogluconate dehydrogenase, decarboxylating (EC 1.1.1.44). [Ovis aries] ACD8_HUMAN Acyl-CoA dehydrogenase family member 8, mitochondrial precursor (EC 1.3.99.-) (ACAD-8) (Isobutyryl- CoA dehydrogenase) (Activator- recruited cofactor 42 kDa component) (ARC42). [Homo sapiens]

ACDBJHUMAN Acyl-CoA dehydrogenase, short/branched chain specific, mitochondrial precursor (EC 1.3.99.-) (SBCAD) (2- methyl branched chain acyl-CoA dehydrogenase) (2-MEBCAD) (2-methylbutyryl-coenzyme A dehydrogenase) (2-methylbutyryl-CoA dehydrogenase). [Homo sapiens] ACDB_MOUSE Acyl-CoA dehydrogenase, short/branched chain specific, mitochondrial precursor (EC 1.3.99.-) (SBCAD) (2- methyl branched chain acyl-CoA dehydrogenase) (2-MEBCAD) (2-methylbutyryl-coenzyme A dehydrogenase) (2-methylbutyryl-CoA dehydrogenase). [Mus musculus]

ACDM. .MOUSE Acyl-CoA dehydrogenase, medium-chain specific, mitochondria] precursor (EC 1.3.99.3) (MCAD). [Mus musculus]

ACDS_ MOUSE Acyl-CoA dehydrogenase, short-chain specific, mitochondrial precursor (EC 1.3.99.2) (SCAD) (Butyryl-CoA dehydrogenase). [Mus musculus]

ACDS_ RAT Acyl-CoA dehydrogenase, short-chain specific ACDV HUMAN Acyl-CoA dehydrogenase, very-long-chain specific, mitochondrial precursor (EC 1.3.99.-) (VLCAD). [Homo sapiens]

ACDV_MOUSE Acyl-CoA dehydrogenase, very-long-chain specific, mitochondrial precursor (EC 1.3.99.-) (VLCAD) (MVLCAD). [Mus musculus]

ADH1_ RABIT Alcohol dehydrogenase alpha chain (EC 1.1.1.1) (ADH). [Oryctolagus cuniculus] ADH6_ HUMAN Alcohol dehydrogenase 6 (EC 1.1.1.1). [Homo sapiens] ADHA. PERMA Alcohol dehydrogenase A chain (EC 1.1.1.1). [Peromyscus manlcul ADHX..RAT Alcohol dehydrogenase class III (EC 1.1.1.1) (Alcohol dehydrogenase 2) (Glutathlone-dependent formaldehyde dehydrogenase) (EC 1.2.1.1) (FDH) (FALDH) (Alcohol dehydrogenase-B2). [Rattus norvegicus]

ADH_MACMU Alcohol dehydrogenase alpha chain (EC 1.1.1.1) (ADH). [Macaca mulatta] AKBA_HUMAN Aldo-keto reductase family 1 member BIO (EC 1.1.1.-) (Aldose reductase-llke) (ARL-1) (Small intestine reductase) (SI reductase) (Aldose reductase-related protein) (ARP) (hARP). [Homo sapiens]

AKC1_HUMAN Aldo-keto reductase family 1 member CI (EC 1.1.1.-) (Trans-1,2- dlhydrobenzene-l,2-diol dehydrogenase) (EC 1.3.1.20) (Hlgh-afflnlty hepatic bile acid-binding protein) (HBAB) (Chlordecone reductase homolog HAKRC) (Dihydrodlol dehydrogenase 2) (DD2) (20 alp

AKD1_RAT 3-oxo-5-beta-sterold 4-dehydrogenase (EC 1.3.99.6) (Delta(4)-3- ketosteroid 5-beta-reductase) (Aldo- keto reductase family 1 member Dl). [Rattus norvegicus]

AR71_RAT Aflatoxln BI aldehyde reductase (EC 1.-.-.-) (AFB1-AR). [Rattus norvegicus] AR72_HU AN Aflatoxin BI aldehyde reductase 1 (EC 1.-.-.-) (AFB1-AR 1) (Aldoketoreductase 7). [Homo sapiens] BIEA_HUMAN Biliverdin reductase A precursor (EC 1.3.1.24) (Billverdin-IX alpha- reductase). [Homo sapiens] C26A_HUMAN Cytochrome P450 26A2 (EC 1.14.-.-) (P450RAI-2) (Retinoic-acid metabolizing cytochrome). [Homo sapiens]

C343_HUMAN Cytochrome P450 3A43 (EC 1.14.14.1). [Homo sapiens]

CA01_HUMAN Acyl-coenzyme A oxidase 1, peroxiso al (EC 1.3.3.6) (Palmitoyl -CoA oxidase) (AOX). [Homo sapiens]

CA01_RAT Acyl-coenzyme A oxidase 1, peroxisomal (EC 1.3.3.6) (Palmitoyl-CoA oxidase) (AOX). [Rattus norvegicus]

COXB_HUMAN Cytochrome c oxidase polypeptide Vb, mitochondrial precursor (EC 1.9.3.1). [Homo sapiens]

COXB_MOUSE Cytochrome c oxidase polypeptide Vb, mitochondrial precursor (EC 1.9.3.1). [Mus musculus] COXD_RAT Cytochrome c oxidase polypeptide Vla-heart, mitochondrial precursor (EC 1.9.3.1) (COXVIAH) (Fragment).

[Rattus norvegicus] COXE_RAT Cytochrome c oxidase polypeptide Vla-liver, mitochondrial precursor (EC 1.9.3.1). [Rattus norvegicus]

COXI_MOUSE Cytochrome c oxidase polypeptide VIc-2 (EC 1.9.3.1). [Mus musculus] CP42_RAT Cytochrome P450 4A2 precursor (EC 1.14.15.3) (CYPIVA2) (Laurie acid omega-hydroxylase) (P450-LA- omega 2) (P450 K-5) (P-450 K-2). [Rattus norvegicus] CP4YJHUMAN Cytochrome P450 4A11 precursor (EC 1.14.15.3) (CYPIVA11) (Fatty acid omega-hydroxylase) (P-450 HK omega) (Laurie acid omega-hydroxylase) (CYP4AII) (P450-HL-omega). [Homo sapiens] CPC6_RAT Cytochrome P450 2C6 (EC 1.14.14.1) (CYPIIC6) (P450 PB1) (PTF2). [Rattus norvegicus]

CTP1_HUMAN C-terminal binding protein 1 (CtBPl). [Homo sapiens] CX41_HUMAN Cytochrome c oxidase subunit IV isoform 1, mitochondrial precursor (EC 1.9.3.1) (COX IV-1) (Cytochrome c oxidase polypeptide IV). [Homo sapiens] D3HI_RAT 3-hydroxylsobutyrate dehydrogenase, mitochondrial precursor (EC 1.1.1.31) (HIBADH). [Rattus norvegicus] D7A1_HUMAN Aldehyde dehydrogenase family 7 member Al (EC 1.2.1.3) (Antiquitin 1). [Homo sapiens] D7A1_RAT Aldehyde dehydrogenase family 7 member Al (EC 1.2.1.3) (Antiquitin 1) (Fragment). [Rattus norvegicus]

DECRJHUMAN 2,4-dienoyl-CoA reductase, mitochondrial precursor (EC 1.3.1.34) (2,4- dienoyl-CoA reductase [NADPH])

(4-enoyl-CoA reductase [NADPH]). [Homo sapiens] DH3I_MOUSE 3-hydroxyisobutyrate dehydrogenase, mitochondrial precursor (EC 1.1.1.31) (HIBADH). [Mus musculus] DHAl_MOUSE Aldehyde dehydrogenase 1A1 (EC 1.2.1.3) (Aldehyde dehydrogenase, cytosolic) (ALDH class 1) (ALHDII)

(ALDH-E1). [Mus musculus] DHA5_HUMAN Aldehyde dehydrogenase X, mitochondrial precursor (EC 1.2.1.3) (ALDH class 2). [Homo sapiens] DHA6JHUMAN Aldehyde dehydrogenase 6 (EC 1.2.1.5). [Homo sapiens] DHA7_HUMAN Aldehyde dehydrogenase 7 (EC 1.2.1.5). [Homo sapiens] DHAG_HUMAN Aldehyde dehydrogenase, E3 isozyme (EC 1.2.1.3) (Gamma- aminobutyraldehyde dehydrogenase) (EC

1.2.1.19) (R-aminobutyraldehyde dehydrogenase). [Homo sapiens] DHAM_HUMAN Aldehyde dehydrogenase, mitochondrial precursor (EC 1.2.1.3) (ALDH class 2) (ALDHI) (ALDH-E2). [Homo sapiens] DHAM_MOUSE Aldehyde dehydrogenase, mitochondrial precursor (EC 1.2.1.3) (ALDH class 2) (AHD-M1) (ALDHI) (ALDH-

E2). [Mus musculus] DHB2_HUMAN Estradiol 17 beta-dehydrogenase 2 (EC 1.1.1.62) (17-beta-HSD 2) (Microsomal 17-beta-hydroxysteroid dehydrogenase) (20 alpha- hydroxysteroid dehydrogenase) (20-alpha-HSD) (E2DH). [Homo sapiens] DHB3_HUMAN Estradiol 17 beta-dehydrogenase 3 (EC 1.1.1.62) (17-beta-HSD 3) (Testlcular 17-beta-hydroxysteroid dehydrogenase). [Homo sapiens] DHB3_RAT Estradiol 17 beta-dehydrogenase 3 (EC 1.1.1.62) (17-beta-HSD 3) (Testlcular 17-beta-hydroxysteroid dehydrogenase). [Rattus norvegicus] DHB4J-1UMAN Peroxisomal multifunctional enzyme type 2 (MFE-2) (D-bifunctlonal protein) (DBP) (17-beta-hydroxysteroid dehydrogenase 4) (17-beta-HSD 4) [Includes: D-3-hydroxyacyl-CoA dehydratase (EC 4.2.1.-); 3- hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35)]. [Homo sapie DHE3_BOVIN Glutamate dehydrogenase (EC 1.4.1.3) (GDH). [Bos taurus]

DHE3_HUMAN Glutamate dehydrogenase 1, mitochondrial precursor (EC 1.4.1.3) (GDH). [Homo sapiens] DHE3_MOUSE Glutamate dehydrogenase, mitochondrial precursor (EC 1.4.1.3) (GDH). [Mus musculus] DHE3_RAT Glutamate dehydrogenase, mitochondrial precursor (EC 1.4.1.3) (GD

DHI1_HUMAN Corticosteroid 11-beta-dehydrogenase, isozyme 1 (EC 1.1.1.146) (11-DH) (11-beta-hydroxysteroid dehydrogenase 1) (11-beta-HSDl). [Homo sapiens] DHIl_MOUSE Corticosteroid 11-beta-dehydrogenase, isozyme 1 (EC 1.1.1.146) (11-DH) (11-beta-hydroxysteroid dehydrogenase 1) (11-beta-HSDl) (llbeta- HSD1A). [Mus musculus] DHS2JHUMAN Dehydrogenase/reductase SDR family member 2 (EC 1.1.-.-) (HEP27 protein) (Protein D). [Homo sapiens] DHSA_HUMAN Succlnate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial precursor (EC 1.3.5.1) (Fp)

(Flavoprotein subunit of complex II). [Homo sapiens] DHSOJHUMAN Sorbitol dehydrogenase (EC 1.1.1.14) (L-lditol 2-dehydrogenase). [Homo sapiens] DHSO_MOUSE Sorbitol dehydrogenase (EC 1.1.1.14) (L-lditol 2-dehydrogenase) (Fragment). [Mus musculus] DHSO_RAT Sorbitol dehydrogenase (EC 1.1.1.14) (L-iditol 2-dehydrogenase). [Rattus norvegicus]

DIDH_RAT 3-alpha-hydroxysteroid dehydrogenase (EC 1.1.1.50) (3-alpha-HSD) (Hydroxyprostaglandin dehydrogenase). [Rattus norvegicus] DLDHJHUMAN Dihydrolipoamlde dehydrogenase, mitochondrial precursor (EC 1.8.1.4) (Glycine cleavage system L protein). [Homo sapiens] DLDH_MOUSE Dihydrolipoamlde dehydrogenase, mitochondrial precursor (EC 1.8.1.4). [Mus musculus] DPYD_BOVIN Dihydropyrimidine dehydrogenase [NADP+] (EC 1.3.1.2) (DPD) (DHPDHase) (Dihydrouracll dehydrogenase) (Dihydrothymine dehydrogenase). [Bos taurus] DPYD_HUMAN Dihydropyrimidine dehydrogenase [NADP+] precursor (EC 1.3.1.2) (DPD) (DHPDHase) (Dihydrouracll dehydrogenase) (Dihydrothymine dehydrogenase). [Homo sapiens] ECHA_HUMAN TrifUnctional enzyme alpha subunit, mitochondrial precursor (TP-alpha) (78 kDa gastrln-binding protein)

[Includes: Long-chain enoyl-CoA hydratase (EC 4.2.1.17); Long chain 3-hydroxyacyl-CoA dehydrogenase

(EC 1.1.1.35)]. [Homo sapiens] ECHA_PIG TrifUnctional enzyme alpha subunit, mitochondrial precursor (TP-alpha) (78 kDa gastrin-binding protein)

(EC 1.1.1.35)]. [Sus scrofa] ECHA_RAT TrifUnctional enzyme alpha subunit, mitochondrial precursor (TP-alpha) [Includes: Long-chain enoyl-CoA hydratase (EC 4.2.1.17); Long chain 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35)]. [Rattus norvegicus] ECHB_HUMAN TrifUnctional enzyme beta subunit, mitochondrial precursor (TP-beta) [Includes: 3-ketoacyl-CoA thiolase

(EC 2.3.1.16) (Acetyl-CoA acyltransferase) (Beta-ketothlolase)]. [Homo sapiens] ECHP_CAVPO Peroxisomal bifuηctional enzyme (PBE) (PBFE) [Includes: Enoyl-C ECHP_MOUSE Peroxisomal bifunctional enzyme (PBE) (PBFE) [Includes: Enoyl-C ER29JHUMAN Endoplasmic reticulum protein ERp29 precursor (ERp31) (ERp28). [Homo sapiens] ERG1_HUMAN Squalene monooxygenase (EC 1.14.99.7) (Squalene epoxidase) (SE). [Homo sapiens] FASJHUMAN Fatty acid synthase (EC 2.3.1.85) [Includes: EC 2.3.1.38; EC 2.3.1.39; EC 2.3.1.41; EC 1.1.1.100; EC

4.2.1.61; EC 1.3.1.10; EC 3.1.2.14], [Homo sapiens] FAS_RAT Fatty acid synthase (EC 2.3.1.85) [Includes: EC 2.3.1.38; EC 2.3.1.39; EC 2.3.1.41; EC 1.1.1.100; EC

4.2.1.61; EC 1.3.1.10; EC 3.1.2.14]. [Rattus norvegicus] FCLJHUMAN GDP-L-fucose synthetase (EC 1.1.1.271) (FX protein) (Red cell NADP(H)- binding protein) (GDP-4-keto-6- deoxy-D-mannose-3,5-epimerase-4- reductase). [Homo sapiens] FCL_MOUSE GDP-L-fucose synthetase (EC 1.1.1.271) (FX protein) (Red cell NADP(H)- binding protein) (GDP-4-keto-6- deoxy-D-mannose-3,5-epimerase-4- reductase) (Transplantation antigen P35B) (Tum-P35B antigen). [Mus musculus] FM01_RAT Dimethylaniline monooxygenase [N-oxide forming] 1 (EC 1.14.13.8) (Hepatic flavin-containing monooxygenase 1) (FMO 1) (Dimethylaniline oxidase 1). [Rattus norvegicus] FOX2_NEUCR Peroxisomal hydratase-dehydrogenase-epimer

FTDH_HUMAN 10-formyltetrahydrofolate dehydrogenase (EC 1.5.1.6) (10-FTHFDH). [Homo sapiens] FTDH_MOUSE 10-formyltetrahydrofolate dehydrogenase (EC 1.5.1.6) (10-FTHFDH). [Mus musculus] FTDH_RAT 10-formyltetrahydrofolate dehydrogenase (EC 1.5.1.6) (10-FTHFDH) (FBP-CI). [Rattus norvegicus]

G3P1_HUMAN Glyceraldehyde 3-phosphate dehydrogenase, muscle (EC 1.2.1.12) (GAPDH). [Homo sapiens] G3P1JACOR Glyceraldehyde 3-phosphate dehydrogenase, muscle (EC 1.2.1.12)

G3P_BOVIN Glyceraldehyde 3-phosphate dehydrogenase (EC 1.2.1.12) (GAPDH) (Fragment). [Bos taurus]

G3P_MESAU Glyceraldehyde 3-phosphate dehydrogenase (EC 1.2.1.12) (GAPDH) (

G3P_RAT Glyceraldehyde 3-phosphate dehydrogenase (EC 1.2.1.12) (GAPDH) (38 kDa BFA-dependent ADP- ribosylation substrate) (BARS-38). [Rattus norvegicus] G6PD_HUMAN Glucose-6-phosphate 1-dehydrogenase (EC 1.1.1.49) (G6PD). [Homo sapiens] GLS1_ARATH Ferredoxin-dependent glutamate synthase 1 GST3JHUMAN Microsomal glutathlone S-transferase 3 (EC 2.5.1.18) (Microsomal GST- 3) (Microsomal GST-III). [Homo sapiens] GTK1_RAT Glutathione S-transferase, mitochondrial (GS

HCD2JHUMAN 3-hydroxyacyl-CoA dehydrogenase type II (EC 1.1.1.35) (Type II HADH) (Endoplasmic reticulum- associated amyloid beta-peptide binding protein) (Short-chain type dehydrogenase/reductase XH98G2).

[Homo sapiens] HCD2_RAT 3-hydroxyacyl-CoA dehydrogenase type II (EC 1.1.1.35) (Type II HADH) (Endoplasmic reticulum- assoclated amyloid beta-peptide binding protein). [Rattus norvegicus] HCDH HUMAN Short chain 3-hydroxyacyl-CoA dehydrogenase, mitochondrial precursor (EC 1.1.1.35) (HCDH) (Medium and short chain L-3-hydroxyacyl-coenzyme A dehydrogenase). [Homo sapiens] HCDH_MOUSE Short chain 3-hydroxyacyl-CoA dehydrogenase, mitochondrial precursor (EC 1.1.1.35) (HCDH) (Medium and short chain L-3-hydroxyacyl-coenzyme A dehydrogenase). [Mus musculus] HCDH_RAT Short chain 3-hydroxyacyl-CoA dehydrogenase, mitochondrial precursor (EC 1.1.1.35) (HCDH) (Medium and short chain L-3-hydroxyacyl-coenzyme A dehydrogenase). [Rattus norvegicus] HEM6JHUMAN Coproporphyrinogen III oxidase, mitochondrial precursor (EC 1.3.3.3) (Coproporphyrinogenase) (Coprogen oxidase) (COX). [Homo sapiens] HMDH_HUMAN 3-hydroxy-3-methylglutaryl-coenzyme A reductase (EC 1.1.1.34) (HMG-CoA reductase). [Homo sapiens] H01_HUMAN Heme oxygenase 1 (EC 1.14.99.3) (Hθ-1). [Homo sapiens] H02_HUMAN Heme oxygenase 2 (EC 1.14.99.3) (Hθ-2). [Homo sapiens] HPPD_MOUSE 4-hydroxyphenylpyruvate dioxygenase (EC 1.13.11.27) (4HPPD) (HPD) (HPPDase) (F protein) (F

Alloantigen). [Mus musculus] HPPD_RAT 4-hydroxyphenylpyruvate dioxygenase (EC 1.13.11.27) (4HPPD) (HPD) (HPPDase) (F protein) (F alloantigen) (Fragment). [Rattus norvegicus] IDH1_KLULA Isocitrate dehydrogenase [NAD] subunit 1, IDHAJHUMAN Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial precursor (EC 1.1.1.41) (Isocltrlc dehydrogenase) (NAD+-specific ICDH). [Homo sapiens] IDHC_HUMAN Isocitrate dehydrogenase [NADP] cytoplasmic (EC 1.1.1.42) (Oxalosuccinate decarboxylase) (IDH)

(NADP+-specific ICDH) (IDP). [Homo sapiens] IDHC_MICME Isocitrate dehydrogenase [NADP] cytoplasmic (EC 1.1.1.42) (Oxal IDHC_RAT Isocitrate dehydrogenase [NADP] cytoplasmic (EC 1.1.1.42) (Oxalosuccinate decarboxylase) (IDH)

(NADP+-specific ICDH) (IDP). [Rattus norvegicus] IDHC_TOBAC ISOCITRATE DEHYDROGENASE [NADP] (OXALOSUCC IDHP_BOVIN Isocitrate dehydrogenase [NADP], mitochondrial precursor (EC 1.1.1.42) (Oxalosuccinate decarboxylase)

(IDH) (NADP+-specific ICDH) (IDP) (ICD-M). [Bos taurus] IDHPJHUMAN Isocitrate dehydrogenase [NADP], mitochondrial precursor (EC 1.1.1.42) (Oxalosuccinate decarboxylase)

(IDH) (NADP+-specific ICDH) (IDP) (ICD-M). [Homo sapiens] IDHP_MOUSE Isocitrate dehydrogenase [NADP], mitochondrial precursor (EC 1.1.1.42) (Oxalosuccinate decarboxylase)

(IDH) (NADP+-specific ICDH) (IDP) (ICD-M). [Mus musculus] IDH_COREF Isocitrate dehydrogenase [NADP] (Oxalosucc

IMD1_HUMAN Inosine-5'-monophosphate dehydrogenase 1 (EC 1.1.1.205) (IMP dehydrogenase 1) (IMPDH-I) (IMPD 1).

[Homo sapiens] IMDl_MOUSE Inosine-5'-monophosphate dehydrogenase 1 (EC 1.1.1.205) (IMP dehydrogenase 1) (IMPDH-I) (IMPD 1).

[Mus musculus] IMD2_HUMAN Inosine-5'-monophosphate dehydrogenase 2 (EC 1.1.1.205) (IMP dehydrogenase 2) (IMPDH-II) (IMPD 2).

[Homo sapiens] IMD2_MESAU Inosine-5'-monophosphate dehydrogenase 2 (EC 1.1.1.205) (IMP de IMD2_MOUSE Inosine-5'-monophosphate dehydrogenase 2 (EC 1.1.1.205) (IMP dehydrogenase 2) (IMPDH-II) (IMPD 2).

[Mus musculus] IVD_HUMAN Isovaleryl-CoA dehydrogenase, mitochondrial precursor (EC 1.3.99.10) (IVD). [Homo sapiens]

LAJHUMAN Lupus La protein (Sjogren syndrome type B antigen) (SS-B) (La rlbonucleoprotein) (La autoantigen).

[Homo sapiens] LDHA_RAT L-lactate dehydrogenase A chain (EC 1.1.1.27) (LDH-A) (LDH muscle subunit) (LDH-M). [Rattus norvegicus] LEU3_CANGA 3-isopropylmalate dehydrogenase (Beta-IPM LOX5_MESAU Arachidonate 5-llpoxygenase (EC 1.13.11.34) (5-lipoxygenase) (5 LOX5_MOUSE Arachidonate 5-lipoxygenase (EC 1.13.11.34) (5-lipoxygenase) (5-LO). [Mus musculus] LOX5_RAT Arachidonate 5-lipoxygenase (EC 1.13.11.34) (5-lipoxygenase) (5-LO). [Rattus norvegicus]

LOXPJHUMAN Arachidonate 12-lipoxygenase, 12S-type (EC 1.13.11.31) (12-LOX) (Platelet-type lipoxygenase 12). [Homo sapiens] LXE3_HUMAN Epidermis-type lipoxygenase 3 (EC 1.13.11.-) (e-LOX-3). [Homo sapiens]

M2GD_RAT Dimethylglycine dehydrogenase, mitochondrial precursor (EC 1.5.99.2) (ME2GLYDH). [Rattus norvegicus]

MAOM_HUMAN NAD-dependent malic enzyme, mitochondrial precursor (EC 1.1.1.3

MDHC_PIG Malate dehydrogenase, cytoplasmic (EC 1.1.1.37). [Sus scrofa]

MDHM_HUMAN Malate dehydrogenase, mitochondrial precursor (EC 1.1.1.37). [Homo sapiens]

MDHM_MOUSE Malate dehydrogenase, mitochondrial precursor (EC 1.1.1.37). [Mus musculus]

MDHM_RAT Malate dehydrogenase, mitochondrial precursor (EC 1.1.1.37). [Rattus norvegicus]

MMSAJHUMAN Methylmalonate-semlaldehyde dehydrogenase [acylating], mitochondrial precursor (EC 1.2.1.27)

(MMSDH). [Homo sapiens]

MMSA_RAT Methylmalonate-semlaldehyde dehydrogenase [acylating], mitochondrial precursor (EC 1.2.1.27)

(MMSDH). [Rattus norvegicus]

MTDH_ARATH Probable mannitol dehydrogenase (NAD-depen NAPA_ALCEU PERIPLASMIC NITRATE REDUCTASE PRECURSOR NIA_USTMA Nitrate reductase [NADPH] (NR) N0S1_HUMAN Nitric-oxide synthase, brain (EC 1.14.13.39) (NOS, type I) (Neuronal NOS) (N-NOS) (nNOS) (Constitutive

NOS) (NC-NOS) (bNOS). [Homo sapiens]

NS2A_HUMAN Nitric oxide synthase, Inducibie (EC 1.14.13.39) (NOS, type II) (Inducibie NOS) (INOS) (Hepatocyte NOS)

(HEP-NOS). [Homo sapiens]

NSDL_HUMAN NAD(P)-dependent steroid dehydrogenase (EC 1.1.1.-) (H105e3 protein). [Homo sapiens] ODBA_HUMAN 2-oxoisovalerate dehydrogenase alpha subunit, mitochondrial precursor (EC 1.2.4.4) (Branched-chaln alpha-keto acid dehydrogenase El component alpha chain) (BCKDH El-alpha). [Homo sapiens]

OD01_HUMAN 2-oxoglutarate dehydrogenase El component, mitochondrial precursor (EC 1.2.4.2) (Alpha-ketoglutarate dehydrogenase). [Homo sapiens]

OXLA_CROAD L-amino acid oxidase precursor (LAO) (LAAO PAHX_RAT Phytanoyl-CoA dioxygenase, peroxisomal precursor (EC 1.14.11.18) (Phytanoyl-CoA alpha-hydroxylase)

(PhyH) (Phytanic acid oxidase). [Rattus norvegicus]

PCD8_HUMAN Programmed cell death protein 8, mitochondrial precursor (EC 1. PCD8_MOUSE Programmed cell death protein 8, mitochondrial precursor (EC 1.-.-.-) (Apoptosis-iπducing factor). [Mus musculus]

PDA3_HUMAN Protein disulfide Isomerase A3 precursor (EC 5.3.4.1) (Disulfide isomerase ER-60) (ERp60) (58 kDa microsomal protein) (p58) (ERp57) (58 kDa glucose regulated protein). [Homo sapiens]

PDA3_MOUSE Protein disulfide isomerase A3 precursor (EC 5.3.4.1) (Disulfide isomerase ER-60) (ERp60) (58 kDa microsomal protein) (p58) (ERp57). [Mus musculus]

PDA3_RAT Protein disulfide isomerase A3 precursor (EC 5.3.4.1) (Disulfide isomerase ER-60) (ERp60) (58 kDa microsomal protein) (p58) (ERp57) (HIP-70) (Q-2). [Rattus norvegicus]

PDA4_HUMAN Protein disulfide isomerase A4 precursor (EC 5.3.4.1) (Protein ERp-72) (ERp72). [Homo sapiens] PDA5_HUMAN Protein disulfide isomerase AS precursor (EC 5 3.4.1) (Protein disulfide isomerase-related protein). [Homo sapiens]

PDA6_HUMAN Protein disulfide isomerase A6 precursor (EC 5 3.4.1) (Protein disulfide isomerase P5). [Homo sapiens] PDA6_RAT Protein disulfide isomerase A6 precursor (EC 5.3.4.1) (Protein disulfide isomerase P5) (Calcium-binding protein 1) (CaBPl) (Fragment) [Rattus norvegicus]

PDI_BOVIN Protein disulfide isomerase precursor (PDI) (EC 5.3.4.1) (Prolyl 4- hydroxylase beta subunit) (Cellular thyroid hormone binding protein) (P55). [Bos taurus]

PDI_HUMAN Protein disulfide isomerase precursor (PDI) (EC 5.3.4.1) (Prolyl 4- hydroxylase beta subunit) (Cellular thyroid hormone binding protein) (P55). [Homo sapiens]

PDI_MOUSE Protein disulfide isomerase precursor (PDI) (EC 5.3.4.1) (Prolyl 4- hydroxylase beta subunit) (Cellular thyroid hormone binding protein) (P55) (ERP59). [Mus musculus]

PDI RAT Protein disulfide isomerase precursor (PDI) (EC 5.3 4.1) (Prolyl 4- hydroxylase beta subunit) (Cellular thyroid hormone binding protein) (Thyroxme deiodlnase) (EC 3.8.1 4) (Iodothyronine 5'-monodeiodιnase)

(5'-MD). [Rattus norvegicus]

PDX1_HUMAN Peroxiredoxin 1 (EC 1.11.1.-) (Thioredoxin peroxidase 2) (Thioredoxin- dependent peroxide reductase 2)

(Proliferation-associated protein PAG) (Natural killer cell enhancing factor A) (NKEF-A). [Homo sapiens] PDX1_M0USE Peroxiredoxin 1 (EC 1.11.1.-) (Thioredoxin peroxidase 2) (Thioredoxin- dependent peroxide reductase 2)

(Osteoblast specific factor 3) (OSF-3) (Macrophage 23 kDa stress protein). [Mus musculus] PDX1_RAT Peroxiredoxin 1 (EC 1.11.1.-) (Thioredoxin peroxidase 2) (Thioredoxin- dependent peroxide reductase 2)

(Heme-blnding 23 kDa protein) (HBP23). [Rattus norvegicus] PDX2_HUMAN Peroxiredoxin 2 (EC 1.11.1.-) (Thioredoxin peroxidase 1) (Thioredoxin- dependent peroxide reductase 1)

(Thiol-specific antioxidant protein) (TSA) (PRP) (Natural killer cell enhancing factor B) (NKEF-B). [Homo sapiens]

PDX4_MOUSE Peroxiredoxin 4 (EC 1.11.1.-) (Prx-IV) (Thioredoxin peroxidase A0372) (Thloredoxin-dependent peroxide reductase A0372) (Antioxidant enzyme AOE372). [Mus musculus]

PE2R_RAT 20-alpha-hydroxysteroid dehydrogenase (EC 1.1.1.149) (20-alpha-HSD) (HSD1). [Rattus norvegicus]

PERL_HUMAN Lactoperoxidase precursor (EC 1.11.1.7) (LPO) (Salivary peroxidase) (SPO). [Homo sapiens]

PERM_HUMAN Myeloperoxidase precursor (EC 1.11.1.7) (MPO). [Homo sapiens]

PERT_HUMAN Thyroid peroxidase precursor (EC 1.11 1.8) (TPO). [Homo sapiens]

PGH1_HUMAN Prostaglandin G/H synthase 1 precursor (EC 1.14.99.1) (Cyclooxygenase -1) (COX-1) (Prostaglandln- endoperoxide synthase 1) (Prostaglandin H2 synthase 1) (PGH synthase 1) (PGHS-1) (PHS 1). [Homo sapiens]

PL01_MOUSE Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 precursor (EC PL02_HUMAN Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2 precursor (EC 1.14.11.4) (Lysyl hydroxylase 2) (LH2).

[Homo sapiens]

PLθ3_HUMAN Procollagen-lyslne,2-oxoglutarate 5-dioxygenase 3 precursor (EC 1.14.11.4) (Lysyl hydroxylase 3) (LH3).

[Homo sapiens]

PROC_HUMAN Pyrrollne-5-carboxylate reductase (EC 1.5.1.2) (P5CR) (P5C reductase). [Homo sapiens] PUT2_HUMAN Delta-l-pyrroline-5-carboxylate dehydrogenase, mitochondrial precursor (EC 1.5.1.12) (P5C dehydrogenase). [Homo sapiens]

Q14400 GLUD1 protein (Fragment). [Homo sapiens] Q811C4 Dihydrolipoamlde dehydrogenase precursor (EC 1.8.1.4) (Fragment). [ Q8K417 Glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12) (GAPDH) (Fra Q9N2D6 Glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12) (GAPDH). [Ca RIR1_HUMAN Rlbonucleoside-diphosphate reductase Ml chain (EC 1.17.4.1) (Ribonucleotide reductase large chain).

[Homo sapiens] R0H1_RAT Retinol dehydrogenase type I (EC 1.1.1.105) (RODH I). [Rattus norvegicus]

SERA_HUMAN D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95) (3-PGDH). [Homo sapiens] SSDH_HUMAN Succinate semialdehyde dehydrogenase, mitochondrial precursor (EC 1.2.1.24) (NAD(+)-dependent succinic semialdehyde dehydrogenase). [Homo sapiens] SSDH_RAT Succinate semialdehyde dehydrogenase (EC 1.2.1.24) (NAD(+)-dependent succinic semialdehyde dehydrogenase). [Rattus norvegicus] T230_HUMAN Tryptophan 2,3-dioxygenase (EC 1.13.11.11) (Tryptophan pyrrolase) (Tryptophanase) (Tryptophan oxygenase) (Tryptamin 2,3-dloxygenase) (TRPO). [Homo sapiens] THIMJHUMAN 3-ketoacyl-CoA thiolase, mitochondrial (EC 2.3.1.16) (Beta- ketothlolase) (Acetyl-CoA acyltransferase)

(Mitochondrial 3-oxoacyl- CoA thiolase) (TI). [Homo sapiens] TXNL_HUMAN Thioredoxln-like protein (32 kDa thioredoxin-related protein). [Homo sapiens] UCR2_HUMAN Ubiquinol-cytochrome C reductase complex core protein 2, mitochondrial precursor (EC 1.10.2.2) (Complex

III subunit II). [Homo sapiens] UCR2_MOUSE Ubiquinol-cytochrome C reductase complex core protein 2, mitochondrial precursor (EC 1.10.2.2) (Complex

III subunit II). [Mus musculus] UCRH_MOUSE Ubiquinol-cytochrome C reductase complex 11 kDa protein, mitochondrial precursor (EC 1.10.2.2)

(Mitochondrial hinge protein) (Cytochrome CI, nonheme 11 kDa protein) (Complex III subunit VIII). [Mus musculus] UGDH_MOUSE UDP-glucose 6-dehydrogenase (EC 1.1.1.22) (UDP-Glc dehydrogenase) (UDP-GlcDH) (UDPGDH). [Mus musculus]

Kinase regulators

143S_HUMAN 14-3-3 protein slgma (Stratifin) (Epithelial cell marker protein 1). [Homo sapiens] 143T_HUMAN 14-3-3 protein tau (14-3-3 protein theta) (14-3-3 protein T-cell) (HS1 protein). [Homo sapiens] GLMG_HUMAN Glia maturation factor gamma (GMF-gamma). [Homo sapiens]

Other enzymes

4F2 HUMAN 4F2 cell-surface antigen heavy chain (4F2hc) (Lymphocyte activation antigen 4F2 large subunit) (4F2 heavy chain antigen) (CD98 antigen). [Homo sapiens] 5NTC_HUMAN Cytosolic purine 5'-nucleotldase (EC 3.1.3.5) (5'-nucleotidase 6PGLJHUMAN 6-phosphogluconolactonase (EC 3.1.1.31) (6PGL). [Homo sapiens] AATM_MOUSE Aspartate aminotransferase, mitochondrial precursor (EC 2.6.1.1) (Transaminase A) (Glutamate oxaloacetate transaminase-2). [Mus musculus] ACON_HUMAN Aconitate hydratase, mitochondrial precursor (EC 4.2.1.3) (Citrate hydro-lyase) (Acoπitase). [Homo sapiens] ADA_HUMAN Adenosine deaminase (EC 3.5.4.4) (Adenosine aminohydrolase). [Homo sapiens] AGT2_RAT Alanine—glyoxylate aminotransferase 2, mitochondrial precursor (EC 2.6.1.44) (AGT 2) (Beta-alanine- pyruvate aminotransferase) (Beta- ALAAT II). [Rattus norvegicus] ALFA_RABIT Fructose-bisphosphate aldolase A (EC 4.1.2.13) (Muscle-type aldolase). [Oryctolagus cunicuius]

ALFB_RABIT Fructose-bisphosphate aldolase B (EC 4.1.2.13) (Liver-type aldolase). [Oryctolagus cunicuius] ALFC_MOUSE Fructose-bisphosphate aldolase C (EC 4.1.2.13) (Brain-type aldolase) (Fragment). [Mus musculus] AMD2JHUMAN AMP deaminase 2 (EC 3.5.4.6) (AMP deaminase isoform L). [Homo s AMPB_RAT Aminopeptidase B (EC 3.4.11.6) (Ap-B) (Argiπyl aminopeptidase) (Arglnlne aminopeptidase) (Cytosol amlnopeptidase IV). [Rattus norvegicus] AMPE_HUMAN Glutamyl aminopeptidase (EC 3.4.11.7) (EAP) (Aminopeptidase A) (APA) (Differentiation antigen gpl60).

[Homo sapiens] AMPN_HUMAN Aminopeptidase N (EC 3.4.11.2) (Microsomal aminopeptidase) (GP1

AMYP_MOUSE Alpha-amylase, pancreatic precursor (EC 3.2.1.1) (1,4-alpha-D-glucan glucanohydrolase). [Mus musculus] ANM1_RAT Protein arglnine N-methyltransferase 1 (EC 2.1.1.-). [Rattus norvegicus]

ANM2JHUMAN Protein arginlne N-methyltransferase 2 (EC 2.1.1.-). [Homo sapiens] ANM4JHUMAN Protein arglnine N-methyltransferase 4 (EC 2.1.1.-). [Homo sapiens] ANX3JHUMAN Annexin A3 (Annexin III) (Lipocortin III) (Placental anticoagulant protein III) (PAP-III) (35-alpha calcimedln) (Inositol 1,2-cyclic phosphate 2-phosphohydrolase). [Homo sapiens] AP4A_MOUSE Bis(5'-nucieosyl)-tetraphosphatase (Asymmetrical) (EC 3.6.1.17) (Diadenoslne 5',5'"-Pl,P4-tetraphosphate asymmetrical hydrolase) (Diadenoslne tetraphosphatase) (AP4A hydrolase) (AP4AASE). [Mus musculus] APT_MOUSE Adenine phosphorlbosyltransferase (EC 2.4.2.7) (APRT). [Mus musculus] APT_RAT Adenine phosphoribosyltransferase (EC 2.4.2.7) (APRT). [Rattus norvegicus]

ARDHJHUMAN N-termlnal acetyltransferase complex ARD1 subunit homolog (EC 2.3.1.-). [Homo sapiens] ARGI_MOUSE Arginase 1 (EC 3.5.3.1) (Liver-type arginase). [Mus musculus] ARGI_RAT Arginase 1 (EC 3.5.3.1) (Liver-type arginase). [Rattus norvegicus]

ARHY HUMAN ADP-ribosylarglnlne hydrolase (EC 3.2.2.19) (ADP-ribose-L-arginine cleaving enzyme). [Homo sapiens] ARSBJHUMAN Arylsulfatase B precursor (EC 3.1.6.12) (ASB) (N-acetylgalactosamine- 4-sulfatase) (G4S). [Homo sapiens] ATE1_HUMAN Arginyl-tRNA— protein transferase 1 (EC 2.3.2.8) (R-transferase 1) (Arglnyltransferase 1) (Arginine-tRNA— protein transferase 1). [Homo sapiens] ATPG_HUMAN ATP synthase gamma chain, mitochondrial precursor (EC 3.6.3.14). [Homo sapiens] ATPG_MOUSE ATP synthase gamma chain, mitochondria] precursor (EC 3.6.3.14). [Mus musculus] ATPOJHUMAN ATP synthase oligomycln sensitivity conferral protein, mitochondrial precursor (EC 3.6.3.14) (OSCP).

[Homo sapiens] ATS4_HUMAN ADAMTS-4 precursor (EC 3.4.24.82) (A dlsintegrin and metalioproteinase with thrombospondin motifs 4)

(ADAM-TS 4) (ADAM-TS4) (Aggrecanase 1) (ADMP-1). [Homo sapiens] ATS5_HUMAN ADAMTS-5 precursor (EC 3.4.24.-) (A dlsintegrin and metalioproteinase with thrombospondin motifs 5)

(ADAM-TS 5) (ADAM-TS5) (Aggrecanase-2) (ADMP-2) (ADAM-TS 11). [Homo sapiens] B3G6_HUMAN N-acetyllactosaminide beta-l,3-N-acetylglucosaminyltransferase (EC 2.4.1.149) (Poly-N-acetyllactosamlne extension enzyme) (I-beta- 1,3-N-acetylglucosaminyltransferase) (IGnT) (UDP-GlcNAc:betaGal beta- 1,3-

N-acetylglucosaminyltransferase 6). [Homo sapiens] BACHJHUMAN Cytosolic acyl coenzyme A thloester hydrolase (EC 3.1.2.2) (Long chain acyl-CoA thioester hydrolase)

(CTE-II) (Brain acyl-CoA hydrolase). [Homo sapiens] BAT5_HUMAN Protein BAT5 (HLA-B-associated transcript 5) (NG26 protein) (G5 BAT8JHUMAN Histone-lysine N-methyltransferase, H3 lysιne-9 specific 3 (EC 2.1.1.43) (Histone H3-K9 methyltransferase

3) (H3-K9-HMTase 3) (HLA-B associated transcript 8) (G9a) (NG36). [Homo sapiens] BCA1_ARATH Branched-chain amino acid amlnotransferas

BHMTJHUMAN Betalne— homocysteine S-methyltransferase (EC 2.1.1.5). [Homo sapiens] BHMT_MOUSE Betaine— homocysteine S-methyltransferase (EC 2.1.1.5). [Mus musculus] BHMT_PIG Betaine— homocysteine S-methyltransferase (EC 2.1.1.5) (Fragment). [Sus scrofa]

BHMT_RAT Betaine— homocysteine S-methyltransferase (EC 2.1.1.5). [Rattus norvegicus]

BIR6JHUMAN Baculovlral IAP repeat-containing protein 6 (Ubiquitin-conjugating BIR-domain enzyme apollon). [Homo sapiens] BLMHJHUMAN Bleomycin hydrolase (EC 3.4.22.40) (BLM hydrolase) (BMH) (BH). [Homo sapiens] CACP_HUMAN Carnitine O-acetyltraπsferase (EC 2.3.1.7) (Carnitine acetylase) (CAT). [Homo sapiens] CACP_MOUSE Carnitine O-acetyltransferase (EC 2.3.1.7) (Carnitine acetylase) (CAT). [Mus musculus] CAH3_HUMAN Carbonic anhydrase III (EC 4.2.1.1) (Carbonate dehydratase III) (CA- III). [Homo sapiens] CAH4_MOUSE Carbonic anhydrase IV precursor (EC 4.2.1.1) (Carbonate dehydratase IV) (CA-IV). [Mus musculus] CANIJ-IUMAN Calpain 1, large [catalytic] subunit (EC 3.4.22.52) (Calcium-activated neutral protemase) (CANP) (Mu- type) (muCANP) (Mlcromolar-calpain). [Homo sapiens] CANSJHUMAN Calcium-dependent protease, small subunit (Calpain regulatory subunit) (Calcium-activated neutral protelnase) (CANP). [Homo sapiens] CATB_HUMAN Cathepsln B precursor (EC 3.4.22.1) (Cathepsin BI) (APP secretase) (APPS). [Homo sapiens] CATB_MOUSE Cathepsin B precursor (EC 3.4.22.1) (Cathepsin BI). [Mus musculus] CATD_HUMAN Cathepsin D precursor (EC 3.4.23.5). [Homo sapiens] CATG_HUMAN Cathepsin G precursor (EC 3.4.21.20) (CG). [Homo sapiens] CATHJHUMAN Cathepsin H precursor (EC 3.4.22.16). [Homo sapiens]

CATH_RAT Cathepsin H precursor (EC 3.4.22.16) (Cathepsin B3) (Cathepsin BA). [Rattus norvegicus]

CATZ_HUMAN Cathepsin Z precursor (EC 3.4.22.-) (Cathepsin X) (Cathepsin P). [Homo sapiens] CATZ_RAT Cathepsin Z precursor (EC 3.4.22.-) (Cathepsin Y). [Rattus norvegicus]

CBP2JHUMAN Collagen-binding protein 2 precursor (Colllgln 2) (Rheumatoid arthritis related antigen RA-A47). [Homo sapiens] CBP2_RAT Carboxypeptidase A2 precursor (EC 3.4.17.15). [Rattus norvegicus]

CBPH_HUMAN Carboxypeptidase H precursor (EC 3.4.17.10) (CPH) (Carboxypeptidase E) (CPE) (Enkephaiin convertase)

(Prohormone processing carboxypeptidase). [Homo sapiens] CBPJHUMAN CREB-binding protein (EC 2.3.1.48). [Homo sapiens]

CBS_RAT Cystathionine beta-synthase (EC 4.2.1.22) (Serine sulfhydrase) (Beta-thionase) (Hemoprotein H-450).

[Rattus norvegicus] CETP_HUMAN Cholesteryl ester transfer protein precursor (Lipid transfer protein I). [Homo sapiens] CG16JHUMAN Putative acyl-CoA thioester hydrolase CGI-16 (EC 3.1.2.-). [Homo sapiens] CGL1_HUMAN Cytosolic nonspecific dipeptidase (Glutamate carboxypeptidase-llke protein 1). [Homo sapiens] CISY_HUMAN Citrate synthase, mitochondrial precursor (EC 2.3.3.1). [Homo sapiens] CIXGJ-EUMC CltXG protein [Includes- Apo-citrate lyase CLPPJHUMAN Putative ATP-dependent Clp protease proteolytic subunit, mitochondrial precursor (EC 3.4.21.92)

(Endopeptidase Clp) [Homo sapiens] CN1A_HUMAN Calcium/calmodulin-dependent 3',5'-cyclιc nucleotide phosphodiesterase 1A (EC 3.1.4.17) (Cam-PDE 1A)

(61 kDa Cam-PDE) (hCam-1). [Homo sapiens] CN37_HUMAN 2',3'-cyclic nucleotide 3'-phosphodiesterase (EC 3.1.4.37) (CNP) (CNPase). [Homo sapiens] CN37_MOUSE 2',3'-cyclιc nucleotide 3'-phosphodιesterase (EC 3.1.4.37) (CNP) (CNPase). [Mus musculus] CN3B_HUMAN cGMP-lnhibited 3',5'-cyclic phosphodiesterase B (EC 3.1.4.17) (Cyclic GMP inhibited phosphodiesterase B)

(CGI-PDE B) (CGIPDE1) (CGIP1). [Homo sapiens] CN4AJHUMAN cAMP-specific 3',5'-cyclιc phosphodiesterase 4A (EC 3 1.4 17) (

CN4C_HUMAN cAMP-specific 3',5'-cyclic phosphodiesterase 4C (EC 3.1.4.17) (DPDE1) (PDE21). [Homo sapiens] CN7B_HUMAN cAMP-specific 3',5'-cycllc phosphodiesterase 7B (EC 3.1 4.17). [Homo sapiens] CN9A_HUMAN High-affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A ( CNRB_HUMAN Rod cGMP-specific 3',5'-cyclic phosphodiesterase beta-subunit (EC 3.1 4.17) (GMP-PDE beta). [Homo sapiens] COMT_HUMAN Catechol O-methyltransferase, membrane-bound form (EC 2.1 1.6) (MB-COMT) [Contains: Catechol O- methyltransferase, soluble form (S-COMT)]. [Homo sapiens] CPT1_HUMAN Carnitine O-palmitoyltransferase I, mitochondrial liver Isoform (EC 2.3.1.21) (CPT I) (CPTI-L). [Homo sapiens] CPT2_HUMAN Carnitine O-palmitoyltransferase II, mitochondrial precursor (EC 2.3.1.21) (CPT II). [Homo sapiens] CPT2_MOUSE Carnitine O-palmitoyltransferase II, mitochondrial precursor (EC 2.3.1.21) (CPT II). [Mus musculus] CT13_HUMAN Protein C20orfl3. [Homo sapiens] CYA8_HUMAN Adenylate cyclase, type VIII (EC 4.6.1.1) (ATP pyrophosphate-lyase) (Ca(2+)/calmodulin activated adenylyl cyclase). [Homo sapiens] CYA9JHUMAN Adenylate cyclase, type IX (EC 4.6.1.1) (ATP pyrophosphate-lyase) (Adenylyl cyclase). [Homo sapiens] D3D2_RAT 3,2-trans-enoyl-CoA isomerase, mitochondrial precursor (EC 5.3.3.8) (Dodecenoyl-CoA delta-isomerase).

[Rattus norvegicus] DCE1_FELCA Glutamate decarboxylase, 67 kDa isoform (EC 4.1.1.15) (GAD-67) DCE2_HUMAN Glutamate decarboxylase, 65 kDa isoform (EC 4.1.1.15) (GAD-65) (65 kDa glutamic acid decarboxylase).

[Homo sapiens] DCE2_MOUSE Glutamate decarboxylase, 65 kDa isoform (EC 4.1.1.15) (GAD-65) (65 kDa glutamic acid decarboxylase).

[Mus musculus] DCTD_HUMAN Deoxycytidylate deaminase (EC 3.5.4.12) (dCMP deaminase). [Homo sapiens] DCUP_HUMAN Uroporphyrinogen decarboxylase (EC 4.1.1.37) (URO-D) (UPD). [Homo sapiens] DHYSJHUMAN Deoxyhypusine synthase (EC 2.5.1.46) (DHS). [Homo sapiens] DNM1_HUMAN DNA (cytosine-5)-methyltransferase 1 (EC 2.1.1.37) (Dnmtl) (DNA methyltransferase Hsal) (DNA MTase

Hsal) (MCMT) (M.Hsal). [Homo sapiens] DPD4JHUMAN DNA polymerase delta subunit 4 (DNA polymerase delta subunit pl2). [Homo sapiens] DPOM_HUMAN DNA polymerase mu (EC 2.7.7.7) (Pol Mu). [Homo sapiens] DPY1_RAT Dihydropyrimldinase related protein-1 (DRP-1) (Collapsln response mediator protein 1) (CRMP-1). [Rattus norvegicus] DPY2_HUMAN Dihydropyrimidinase related protein-2 (DRP-2) (Collapsin response mediator protein 2) (CRMP-2) (N2A3).

[Homo sapiens] DPY2_MOUSE Dihydropyrimidinase related protein-2 (DRP-2) (ULIP 2 protein). [Mus musculus] DPY2_RAT Dihydropyrimidinase related protein-2 (DRP-2) (Turned on after division, 64 kDa protein) (TOAD-64)

(Collapsin response mediator protein 2) (CRMP-2). [Rattus norvegicus] DRNGJHUMAN Deoxyribonuciease gamma precursor (EC 3.1.21.-) (DNase gamma) (Deoxyribonuciease I-like 3) (DNase I homolog protein DHP2) (Liver and spleen DNase) (LS-DNase) (LSD). [Homo sapiens] DSRAJHUMAN Double-stranded RNA-specific adenosine deaminase (EC 3.5.4.-) (DRADA) (136 kDa double-stranded RNA binding protein) (P136) (K88DSRBP). [Homo sapiens] DUT_HUMAN Deoxyuridine 5'-triphosphate nucieotidohydrolase, mitochondrial precursor (EC 3.6.1.23) (dUTPase) (dUTP pyrophosphatase). [Homo sapiens] DUT_RAT Deoxyuridine 5'-triphosphate nucieotidohydrolase (EC 3.6.1.23) (dUTPase) (dUTP pyrophosphatase) (PPAR-

Interactlng protein 4) (PIP4). [Rattus norvegicus] E2BG_HUMAN Translation initiation factor eIF-2B gamma subunit (eIF-2B GDP-GTP exchange factor). [Homo sapiens] ECEIJ-IUMAN Endothelin-convertlng enzyme 1 (EC 3.4.24.71) (ECE-1). [Homo sapiens] ECH1JHUMAN Delta3,5-delta2,4-dienoyl-CoA Isomerase, mitochondrial precursor (EC 5.3.3.-). [Homo sapiens] ECHM_HUMAN Enoyl-CoA hydratase, mitochondrial precursor (EC 4.2.1.17) (Short chain enoyl-CoA hydratase) (SCEH)

(Enoyl-CoA hydratase 1). [Homo sapiens] ECHM_RAT Enoyl-CoA hydratase, mitochondrial precursor (EC 4.2.1.17) (Short chain enoyl-CoA hydratase) (SCEH)

(Enoyl-CoA hydratase 1). [Rattus norvegicus] ECPl_MOUSE Eosinophll cationic protein 1 precursor (EC 3.1.27.-) (ECP 1) (Ribonuclease 3-1) (RNase 3-1) (Eosinophil secondary granule ribonuclease-1) (EAR-1). [Mus musculus] EL2_MOUSE Elastase 2 precursor (EC 3.4.21.71). [Mus musculus]

ENOA_RAT Alpha enolase (EC 4.2.1.11) (2-phospho-D-glycerate hydro-lyase) (Non- neural enolase) (NNE) (Enolase

1). [Rattus norvegicus] ENOBJHUMAN Beta enolase (EC 4.2.1.11) (2-phospho-D-glycerate hydro-lyase) (Skeletal muscle enolase) (MSE) (Enolase

3). [Homo sapiens] ENOLJHUMAN Alpha enolase, lung specific (EC 4.2.1.11) (2-phospho-D-glycerate hydro-lyase) (Non-neural enolase)

(NNE) (Phosphopyruvate hydratase) (HLE1). [Homo sapiens] ENP5_HUMAN Ectonucleoside triphosphate diphosphohydrolase 5 precursor (EC 3.6.1.6) (NTPDaseS) (Nucleoside diphosphatase) (CD39 antigen-like 4) (ER-UDPase). [Homo sapiens] ENP5_MOUSE Ectonucleoside triphosphate diphosphohydrolase 5 precursor (EC 3.6.1.6) (NTPDase5) (Nucleoside diphosphatase) (CD39 antigen-like 4) (ER-UDPase). [Mus musculus] EST1_HUMAN Liver carboxylesterase precursor (EC 3.1.1.1) (Acyl coenzyme A:cholesterol acyltransferase) (ACAT)

(Monocyte/macrophage serine esterase) (HMSE) (Serine esterase 1) (Brain carboxylesterase hBrl). [Homo sapiens] ESTDJHUMAN Esterase D (EC 3.1.1.1). [Homo sapiens] EXL3_HUMAN Exostosin-like 3 (EC 2.4.1.223) (Glucuronyl-galactosyi-proteoglycan 4- alpha-N- acetylglucosaminyltransferase) (Putative tumor suppressor protein EXTL3) (Multiple exostosls-like protein

3) (Hereditary multiple exostoses gene isolog) (EXT-related protein 1) EXT2JHUMAN Exostosln-2 (EC 2.4.1.224) (EC 2.4.1.225) (Glucuronosyl-N- acetylglucosaminyl-proteoglycan/N- acetylglucosamlnyl-proteoglycan 4- alpha-N-acetylglucosaminyltransferase) (Putative tumor suppressor protein EXT2) (Multiple exostoses protein 2). [Homo sapiens] F13A_HUMAN Coagulation factor XIII A chain precursor (EC 2.3.2.13) (Protein- glutamine gamma-glutamyltransferase A chain) (Transglutaminase A chain). [Homo sapiens] F16P_HUMAN Fructose-l,6-bisphosphatase (EC 3.1.3.11) (D-fructose-l,6-bisphosphate 1-phosphohydrolase) (FBPase).

[Homo sapiens] F16P_RABIT Fructose-l,6-blsphosphatase (EC 3.1.3.11) (D-fructose-l,6-bisphosphate 1-phosphohydrolase) (FBPase).

[Oryctolagus cunicuius] F16P_RAT Fructose-l,6-bisphosphatase (EC 3.1.3.11) (D-fructose-l,6-blsphosphate 1-phosphohydrolase) (FBPase).

[Rattus norvegicus] F16QJHUMAN Fructose-l,6-bisphosphatase isozyme 2 (EC 3.1.3.11) (D-fructose-1,6- blsphosphate 1-phosphohydrolase)

(FBPase). [Homo sapiens] FAFX_HUMAN Probable ubiquitin carboxyl-terminal hydrolase FAF-X (EC 3.1.2.15) (Ublquitin thiolesterase FAF-X)

(Ubiquitin-specific processing protease FAF-X) (Deublqultinating enzyme FAF-X) (Fat facets protein related,

X-linked) (Ubiquitin-specific protease 9, X chro FBW2_HUMAN F-box/WD-repeat protein 2. [Homo sapiens]

FEN1_HUMAN Flap endonuclease-1 (EC 3.-.-.-) (Maturation factor 1) (MF1). [Homo sapiens] FHIT_HUMAN Bls(5'-adenosyl)-triphosphatase (EC 3.6.1.29) (Diadenoslne 5',5'"- Pl,P3-triphosphate hydrolase)

(Dinucleosidetriphosphatase) (AP3A hydrolase) (AP3AASE) (Fragile histidine triad protein). [Homo sapiens] FK10_MOUSE FK506 binding protein 10 precursor (EC 5.2.1.8) (Peptldyl-prolyl cis- trans Isomerase) (PPIase) (Rotamase)

(65 kDa FK506-blndlng protein) (FKBP65) (Immunophllin FKBP65). [Mus musculus] FKB2JHUMAN FK506-binding protein 2 precursor (EC 5.2.1.8) (Peptldyl-prolyl cis- trans isomerase) (PPIase) (Rotamase)

(13 kDa FKBP) (FKBP-13). [Homo sapiens] FKB3JHUMAN FK506-binding protein 3 (EC 5.2.1.8) (Peptldyl-prolyl cis-trans isomerase) (PPIase) (Rotamase) (25 kDa

FKBP) (FKBP-25) (Rapamycin- selective 25 kDa immunophllin). [Homo sapiens] FKB5_HUMAN FK506-binding protein 5 (EC 5.2.1.8) (Peptldyl-prolyl cis-trans Isomerase) (PPIase) (Rotamase) (51 kDa

FK506-blnding protein) (FKBP- 51) (54 kDa progesterone receptor-associated immunophilin) (FKBP54)

(P54) (FF1 antigen) (HSP90-blnding immunophilin) (Andr FPPS_HUMAN Farnesyl pyrophosphate synthetase (FPP synthetase) (FPS) (Farnesyl diphosphate synthetase) [Includes:

Dlmethylallyltransferase (EC 2.5.1.1); Geranyltranstransferase (EC 2.5.1.10)]. [Homo sapiens] FPPS_RAT Farnesyl pyrophosphate synthetase (FPP synthetase) (FPS) (Farnesyl diphosphate synthetase)

(Cholesterol-regulated 39 kDa protein) (CR 39) [Includes: Dlmethylallyltransferase (EC 2.5.1.1);

Geranyltranstransferase (EC 2.5.1.10)]. [Rattus norvegicus] FUMH_HUMAN Fumarate hydratase, mitochondrial precursor (EC 4.2.1.2) (Fumarase). [Homo sapiens] FUMH_MOUSE Fumarate hydratase, mitochondrial precursor (EC 4.2.1.2) (Fumarase) (EF-3). [Mus musculus] G6NT_HUMAN Beta-l,3-galactosyl-0-glycosyl-glycoprotein beta-l,6-N- acetylglucosaminyltransferase (EC 2.4.1.102)

(Core 2 branching enzyme) (Core2-GlcNAc-transferase) (C2GNT) (Core 2 GNT). [Homo sapiens] G6PIJHUMAN Glucose-6-phosphate isomerase (EC 5.3.1.9) (GPI) (Phosphoglucose isomerase) (PGI) (Phosphohexose isomerase) (PHI) (Neuroieukln) (NLK) (Sperm antigen-36) (SA-36). [Homo sapiens] GABT_HUMAN 4-aminobutyrate aminotransferase, mitochondrial precursor (EC 2.6.1.19) (Gamma-amino-N-butyrate transa inase) (GABA transaminase) (GABA aminotransferase) (GABA-AT) (GABA-T). [Homo sapiens] GALEJ-IUMAN UDP-glucose 4-eplmerase (EC 5.1.3.2) (Galactowaldenase) (UDP- galactose 4-eplmerase). [Homo sapiens] GAMT HUMAN Guanidlnoacetate N-methyltransferase (EC 2.1.1.2). [Homo sapiens] GATM_MOUSE Glycine amidinotransferase, mitochondrial precursor (EC 2.1.4.1) (L- arginιne:glycine amidinotransferase)

(Transamldlnase) (AT). [Mus musculus] GCH1_HUMAN GTP cyclohydrolase I (EC 3.5.4.16) (GTP-CH-I). [Homo sapiens] GCST_HUMAN Aminomethyltransferase, mitochondrial precursor (EC 2.1.2.10) (Glycine cleavage system T protein)

(GCVT). [Homo sapiens] GDE HUMAN Glycogen debranchlng enzyme (Glycogen debrancher) [Includes: 4-alpha- glucanotransferase (EC

2.4.1.25) (θligo-l,4-l,4-glucantransferase); Amylo-alpha-l,6-glucosldase (EC 3.2.1.33) (Amylo-1,6- glucosldase) (Dextrin 6-alpha-D-glucosidase)]. [Homo sapiens] GEPH_RAT Gephyrln (Putative glycine receptor-tubulin linker protein). [Rattus norvegicus]

GL6S_HUMAN N-acetylglucosamιne-6-sulfatase precursor (EC 3.1.6.14) (G6S) (Glucosamlne-6-sulfatase). [Homo sapiens] GL02_HUMAN Hydroxyacylglutathione hydrolase (EC 3.1.2.6) (Glyoxalase II) (GLX II). [Homo sapiens] GL02_M0USE Hydroxyacylglutathione hydrolase (EC 3.1.2.6) (Glyoxalase II) (Glx II). [Mus musculus] GLSK_HUMAN Glutaminase, kidney isoform, mitochondrial precursor (EC 3.5.1.2) (GLS) (L-glutamlne amidohydrolase)

(K-glutaminase). [Homo sapiens] GLSK_RAT Glutaminase, kidney isoform, mitochondrial precursor (EC 3.5.1.2) (GLS) (L-glutamlne amidohydrolase)

(K-glutamlnase). [Rattus norvegicus] GLYM_HUMAN Serine hydroxymethyltransferase, mitochondrial precursor (EC 2.1.2.1) (Serine methylase) (Glycine hydroxymethyltransferase) (SHMT). [Homo sapiens] GMDS HUMAN GDP-mannose 4,6 dehydratase (EC 4.2.1.47) (GDP-D-mannose dehydratase) (GMD). [Homo sapiens] GRAH_HUMAN Granzyme H precursor (EC 3.4.21.-) (Cytotoxic T-lymphocyte protemase) (Cathepsin G-like 2) (CTSGL2)

(CCP-X) (Cytotoxic serine protease-C) (CSP-C). [Homo sapiens] GRL2_RAT Granzyme-like protein II precursor (EC 3.4.21.-). [Rattus norvegicus]

GST1JHUMAN Microsomal glutathlone S-transferase 1 (EC 2.5.1.18) (Microsomal GST- 1) (Microsomal GST-I). [Homo sapiens] GTAl_MOUSE Glutathlone S-transferase Ya chain (EC 2.5.1.18) (GST class-alpha). [Mus musculus] GTA1_RABIT Glutathlone S-transferase alpha I (EC 2.5.1.18) (GSTA1-1) (GST class- alpha). [Oryctolagus cunicuius] GTA2_RAT Glutathlone S-transferase Ya-2 (EC 2.5.1.18) (Llgandin) (Cham 1) (GST class-alpha). [Rattus norvegicus]

GTA3_RAT Glutathlone S-transferase 8 (EC 2.5.1.18) (GST 8-8) (Chain 8) (GST class-alpha) [Rattus norvegicus]

GTC2_RAT Glutathlone S-transferase Yc-2 (EC 2.5.1.18) (Chain 2) (GST Yc2)

GTM2_RAT Glutathlone S-transferase YB2 (Chain 4) (GST

GTM6_MOUSE Glutathlone S-transferase Mu 6 (EC 2.5.1 18) (GST class-mu 6) (Glutathione-S-transferase class M5). [Mus musculus] HDA1_HUMAN Histone deacetylase 1 (HD1). [Homo sapiens] HDA2_HUMAN Histone deacetylase 2 (HD2). [Homo sapiens] HEXB_HUMAN Beta-hexosamlnidase beta chain precursor (EC 3.2 1.52) (N-acetyl-beta- glucosamimdase) (Beta-N- acetylhexosamiπidase) (Hexosaminidase B). [Homo sapiens] HGFA_HUMAN Hepatocyte growth factor activator precursor (EC 3.4.21.-) (HGF activator) (HGFA). [Homo sapiens] HMCMJHUMAN Hydroxymethylglutaryl-CoA synthase, mitochondrial precursor (EC 2.3.3.10) (HMG-CoA synthase) (3- hydroxy-3-methylglutaryl coenzyme A synthase). [Homo sapiens] HMCM_MOUSE Hydroxymethylglutaryl-CoA synthase, mitochondrial precursor (EC 2.3.3.10) (HMG-CoA synthase) (3- hydroxy-3-methylglutaryl coenzyme A synthase) (Fragment). [Mus musculus] HMCM_RAT Hydroxymethylglutaryl-CoA synthase, mitochondrial precursor (EC 2.3.3.10) (HMG-CoA synthase) (3- hydroxy-3-methylglutaryl coenzyme A synthase). [Rattus norvegicus] HMCS_HUMAN Hydroxymethylglutaryl-CoA synthase, cytoplasmic (EC 2.3 3.10) (HMG-CoA synthase) (3-hydroxy-3- methylglutaryl coenzyme A synthase). [Homo sapiens] HMCS_RAT Hydroxymethylglutaryl-CoA synthase, cytoplasmic (EC 2.3 3.10) (HMG-CoA synthase) (3-hydroxy-3- methylglutaryl coenzyme A synthase). [Rattus norvegicus] HMGL_HUMAN Hydroxymethylglutaryl-CoA lyase, mitochondrial precursor (EC 4.1.3.4) (HMG-CoA lyase) (HL) (3-hydroxy-

3-methylglutarate-CoA lyase). [Homo sapiens] HPRT_MUSSP Hypoxanthine-guanine phosphoπbosyltransferase (EC 2.4.2.8) (HGPRT) (HGPRTase) (HPRT A) (Fragment).

[Mus spretus] HPRT_RAT Hypoxanthine-guanine phosphoribosyltransferase (EC 2.4.2.8) (HGPRT) (HGPRTase). [Rattus norvegicus]

HRA1_HUMAN Serine protease HTRA1 precursor (EC 3.4.21.-) (L56). [Homo sapiens] HUTH_RAT Histidine ammonia-lyase (EC 4.3 1.3) (Hlstidase). [Rattus norvegicus]

HYEP_HUMAN Epoxide hydrolase 1 (EC 3.3.2.3) (Microsomal epoxide hydrolase) (Epoxide hydratase). [Homo sapiens] HYES_MOUSE Soluble epoxide hydrolase (SEH) (EC 3.3.2.3) (Epoxide hydratase) (Cytosolic epoxide hydrolase) (CEH).

[Mus musculus] HYES_RAT Soluble epoxide hydrolase (SEH) (EC 3.3.2.3) (Epoxide hydratase) (Cytosolic epoxide hydrolase) (CEH).

[Rattus norvegicus] I1BC_CANFA Interleukin-1 beta convertase precursor (IL-1BC) (EC 3.4.22.36) (IL-1 beta converting enzyme) (ICE)

(Interleukln-1 beta converting enzyme) (P45) (Caspase-1) (CASP-1). [Canls famlliaris] I1BC_RAT Interleukin-1 beta convertase precursor (IL-1BC) (EC 3.4.22.36) (IL-1 beta converting enzyme) (ICE)

(Interleukin-1 beta converting enzyme) (P45) (Caspase-1) (CASP-1). [Rattus norvegicus] ICE6JHUMAN Caspase-6 precursor (EC 3.4.22.-) (Apoptotic protease Mch-2). [Homo sapiens] ICE9_HUMAN Caspase-9 precursor (EC 3.4.22.-) (CASP-9) (ICE-like apoptotic protease 6) (ICE-LAP6) (Apoptotic protease Mch-6) (Apoptotic protease activating factor 3) (APAF-3). [Homo sapiens] ICEA_HUMAN Caspase-10 precursor (EC 3.4.22.-) (ICE-like apoptotic protease 4) (Apoptotic protease Mch-4) (FAS- associated death domain protein interleukln-lB-convertlng enzyme 2) (FLICE2). [Homo sapiens] IPYRJHUMAN Inorganic pyrophosphatase (EC 3.6.1.1) (Pyrophosphate phospho- hydrolase) (PPase). [Homo sapiens] IRE1_HUMAN Iron-responsive element binding protein 1 (IRE-BP 1) (Iron regulatory protein 1) (IRPl) (Ferritin repressor protein) (Aconitate hydratase) (EC 4.2.1.3) (Citrate hydro-lyase) (Aconitase). [Homo sapiens] KYNU_HUMAN Kynurenlnase (EC 3.7.1.3) (L-kynurenine hydrolase). [Homo sapiens] LAGEJHUMAN Glycosyltransferase-like protein LARGE (EC 2.4.-.-) (Acetylglucosaminyltransferase-like protein). [Homo sapiens] LCFA_HUMAN Long-chain-fatty-acid~CoA ligase 1 (EC 6.2.1.3) (Long-chain acyl-CoA synthetase 1) (LACS 1) (Palmitoyl- CoA ligase). [Homo sapiens]

LCFB_MOUSE Long-chain-fatty-acid~CoA ligase 2 (EC 6.2.1.3) (Long-chain acyl-CoA synthetase 2) (LACS 2). [Mus musculus]

LCFB_RAT Long-chaln-fatty-acid~CoA ligase, liver isozyme (EC 6.2.1.3) (Long-chain acyl-CoA synthetase 2) (LACS 2). [Rattus norvegicus]

LCFC_HUMAN Long-chain-fatty-acid— CoA ligase 3 (EC 6.2.1.3) (Long-chain acyl-CoA synthetase 3) (LACS 3). [Homo sapiens]

LCFC_RAT Long-chaln-fatty-acid—CoA ligase 3 (EC 6.2.1.3) (Long-chain acyl-CoA synthetase 3) (LACS 3) (Brain acyl- CoA synthtase II). [Rattus norvegicus]

LCFE_HUMAN Long-chain-fatty-acid-CoA ligase 5 (EC 6.2.1.3) (Long-chain acyl-CoA synthetase 5) (LACS 5). [Homo sapiens]

LCFE_RAT Long-chain-fatty-acid-CoA ligase 5 (EC 6.2.1.3) (Long-chain acyl-CoA synthetase 5) (LACS 5). [Rattus norvegicus]

LCFFJHUMAN Long-chain-fatty-acid-CoA ligase 6 (EC 6.2.1.3) (Long-chain acyl-CoA synthetase 6) (LACS 6). [Homo sapiens]

LEU2_BUCUM 3-isopropyl malate dehydratase large subun LIN1_HUMAN LINE-1 reverse transcriptase homolog. [Homo sapiens] UPL_HUMAN Lipoprotein llpase precursor (EC 3.1.1.34) (LPL). [Homo sapiens] LPH_RAT Lactase-phlorizin hydrolase precursor (Lactase-glycosylceramidase) [Includes: Lactase (EC 3.2.1.108); Phlorlzin hydrolase (EC 3.2.1.62)]. [Rattus norvegicus]

LPPLJHUMAN Eoslnophil lysophosphollpase (EC 3.1.1.5) (Charcot-Leyden crystal protein) (Lysolecithin acylhydrolase) (CLC) (Galactin-10). [Homo sapiens]

LYC_HUMAN Lysozyme C precursor (EC 3.2.1.17) (1,4-beta-N-acetylmuramidase C). [Homo sapiens] M2A1_M0USE Alpha-mannosidase II (EC 3.2.1.114) (Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase) (MAN II) (Golgi alpha-mannosidase II) (Mannosidase alpha class 2A member 1) (AMAN II). [Mus musculus]

M2B1_HUMAN Lysosomal alpha-mannosidase precursor (EC 3.2.1.24) (Mannosidase, alpha B) (Lysosomal acid alpha- mannosidase) (Laman) (Mannosidase alpha class 2B member 1). [Homo sapiens]

MAAI_MOUSE Maleylacetoacetate Isomerase (EC 5.2.1.2) (MAAI) (Glutathlone S- transferase zeta 1) (EC 2.5.1.18) (GSTZ1-1). [Mus musculus]

MCT2_RAT Mast cell protease II precursor (EC 3.4.21.-) (RMCP-II) (RMCP-2) (Group-specific protease). [Rattus norvegicus]

MM08_HUMAN Neutrophil coUagenase precursor (EC 3.4.24.34) (Matrix metalloproteinase-8) (MMP-8) (PMNL coUagenase) (PMNL-CL). [Homo sapiens]

MPB1_HUMAN C-myc promoter-binding protein (MPB-1) (MBP-1). [Homo sapiens]

MR11_RAT Double-strand break repair protein MRE11A (MRE11 homolog 1). [Rattus norvegicus]

MS1P_HUMAN Membrane-bound transcription factor site-1 protease precursor (EC 3.4.21.-) (Site-1 protease) (Subtilisin/kexin-lsozyme-1) (SKI-1). [Homo sapiens]

MTR2JHU AN Myotubularin-related protein 2 (EC 3.1.3.-). [Homo sapiens] MTR6_HU AN Myotubularin related protein 6 (EC 3.1.3.-). [Homo sapiens] MUTA_HUMAN Methylmalonyl-CoA mutase, mitochondrial precursor (EC 5.4.99.2) (MCM). [Homo sapiens] NADC_MOUSE Nicotinate-nucleotide pyrophosphorylase [carboxylating] (EC 2.4.2.19) (Quinolinate phosphoribosyltransferase [decarboxylating]) (QAPRTase) (QPRTase). [Mus musculus]

NAGA_HUMAN Alpha-N-acetylgalactosaminidase precursor (EC 3.2.1.49) (Alpha- galactosldase B). [Homo sapiens] NAR3_HUMAN Ecto-ADP-rlbosyltransferase 3 precursor (EC 2.4.2.31) (NAD(P)(+)~ arginine ADP-ribosyltransferase 3) (Mono(ADP-ribosyl)transferase 3). [Homo sapiens]

NEC2_HUMAN Neuroendocrlne convertase 2 precursor (EC 3.4.21.94) (NEC 2) (PC2) (Prohormone convertase 2) (Proprotein convertase 2) (KEX2-llke endoprotease 2). [Homo sapiens]

NPL1_HUMAN Nucleosome assembly protein 1-like 1 (NAP-1 related protein) (hNRP). [Homo sapiens] NPP1_M0USE Ectonucleotlde pyrophosphatase/phosphodiesterase 1 (E-NPP 1) (Phosphodiesterase I/nucleotide pyrophosphatase 1) (Plasma-cell membrane glycoprotein PC-1) (Ly-41) [Includes: Alkaline phosphodiesterase I (EC 3.1.4.1); Nucleotide pyrophosphatase (EC 3.6.1.9)

NPP1_RAT Ectonucleotlde pyrophosphatase/phosphodiesterase 1 (E-NPP 1) (Phosphodiesterase I/nucleotlde pyrophosphatase 1) (Plasma-cell membrane glycoprotein PC-1) [Includes: Alkaline phosphodiesterase I (EC 3.1.4.1); Nucleotide pyrophosphatase (EC 3.6.1.9) (NPPase)

NPP3 HUMAN Ectonucleotlde pyrophosphatase/phosphodiesterase 3 (E-NPP 3) (Phosphodiesterase I/nucleotide pyrophosphatase 3) (Phosphodiesterase I beta) (PD-Ibeta) (CD203c antigen) [Includes: Alkaline phosphodiesterase I (EC 3.1.4.1); Nucleotide pyrophosphatase (EC 3.6

NPS1_HUMAN NipSnapl protein. [Homo sapiens] NPS1_M0USE NipSnapl protein. [Mus musculus] NPS2_HUMAN NlpSnap2 protein (Glioblastoma amplified sequence). [Homo sapiens] NUD5JHUMAN ADP-sugar pyrophosphatase YSA1H (EC 3.6.1.-) (Nucleoside diphosphate- linked moiety X motif 5) (HSPC115). [Homo sapiens]

NUGL_HUMAN Endonuclease G like 1 (EC 3.1.30.-) (Endo G like). [Homo sapiens] 0CRL_HUMAN Inositol polyphosphate 5-phosphatase OCRL-1 (EC 3.1.3.36) (Lowe's oculocerebrorenal syndrome protein). [Homo sapiens]

ODB2_HUMAN Lipoamide acyltransferase component of branched-chain alpha-keto add dehydrogenase complex, mitochondrial precursor (EC 2.3.1.-) (E2) (Dihydrolipoamlde branched chain transacylase) (BCKAD E2 subunit). [Homo sapiens]

ODB2_MOUSE Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial precursor (EC 2.3.1.-) (E2) (Dihydrolipoamlde branched chain transacylase) (BCKAD E2 subunit). [Mus musculus]

ODθ2_HUMAN Dihydrolipoamlde succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondria] precursor (EC 2.3.1.61) (E2) (E2K). [Homo sapiens]

0DP2_HUMAN Dihydrolipoamlde acetyltransferase component of pyruvate dehydr ODPX_HUMAN Pyruvate dehydrogenase protein X component, mitochondrial precursor (Dihydrolipoamlde dehydrogenase- bindlng protein of pyruvate dehydrogenase complex) (E3-blndlng protein) (E3BP) (proX). [Homo sapiens]

ORN_HUMAN Oligoribonuclease, mitochondria] precursor (EC 3.1.-.-) (Small fragment nuclease) (CGI-114). [Homo sapiens] OTCJ-IUMAN Ornithine carbamoyltransferase, mitochondrial precursor (EC 2.1.3.3) (OTCase) (Ornithine transcarbamylase). [Homo sapiens] OTC_PIG Ornithine carbamoyltransferase, mitochondrial precursor (EC 2.1.3.3) (OTCase) (Ornithine transcarbamylase) (Fragment). [Sus scrofa] OTC_RAT Ornithine carbamoyltransferase, mitochondrial precursor (EC 2.1.3.3) (OTCase) (Ornithine transcarbamylase). [Rattus norvegicus] P2CD_HUMAN Protein phosphatase 2C delta isoform (EC 3.1.3.16) (PP2C-delta) (p53- induced protein phosphatase 1)

(Protein phosphatase magnesium- dependent 1 delta). [Homo sapiens] P2G4JHUMAN Proliferation-associated protein 2G4 (Cell cycle protein p38-2G4 homolog) (hG4-l). [Homo sapiens] P300_HUMAN ElA-associated protein p300 (EC 2.3.1.48). [Homo sapiens] PA1BJ-IUMAN Platelet-activating factor acetylhydrolase IB beta subunit (EC 3.1.1.47) (PAF acetylhydrolase 30 kDa subunit) (PAF-AH 30 kDa subunit) (PAF-AH beta subunit) (PAFAH beta subunit). [Homo sapiens] PA1GJHUMAN Platelet-activating factor acetylhydrolase IB gamma subunit (EC 3.1.1.47) (PAF acetylhydrolase 29 kDa subunit) (PAF-AH 29 kDa subunit) (PAF-AH gamma subunit) (PAFAH gamma subunit). [Homo sapiens] PA26_MOUSE 85 kDa calcium-independent phospholipase A2 (EC 3.1.1.4) (IPLA2) (Cal- PLA2) (Group VI phospholipase

A2) (GVI PLA2). [Mus musculus] PAI1_HUMAN Plasminogen activator inhibitor-1 precursor (PAI-1) (Endothelial plasminogen activator inhibitor) (PAI).

[Homo sapiens] PAPA_HUMAN Pappalysln-1 precursor (EC 3.4.24.79) (Pregnancy-associated plasma proteln-A) (PAPP-A) (Insulin-like growth factor-dependent IGF binding proteln-4 protease) (IGF-dependent IGFBP-4 protease) (IGFBP-4ase).

[Homo sapiens] PCCB_RAT Proplonyl-CoA carboxylase beta chain, mitochondrial precursor (EC 6.4.1.3) (PCCase beta subunit)

(Propanoyl-CoA:carbon dioxide ligase beta subunit). [Rattus norvegicus] PCNA_HUMAN Proliferating cell nuclear antigen (PCNA) (Cyclln). [Homo sapiens] PCNA_MOUSE Proliferating cell nuclear antigen (PCNA) (Cyclin). [Mus musculus] PCNA_RAT Proliferating cell nuclear antigen (PCNA) (Cyclin). [Rattus norvegicus]

PCY2_HUMAN Ethanolamine-phosphate cytidylyltransferase (EC 2.7.7.14) (Phosphorylethanolamine transferase)

(CTP:phosphoethanolamine cytidylyltransferase). [Homo sapiens] PDI2JHUMAN Protein-arginine deiminase type II (EC 3.5.3.15) (Peptidylarglnlne delmlnase II) (PAD-H19). [Homo sapiens] PEX_HUMAN Phosphate regulating neutral endopeptidase (EC 3.4.24.-) (Metalloendopeptidase homolog PEX) (X-linked hypophosphatemia protein) (HYP) (Vitamin D-resistant hypophosphatemic rickets protein). [Homo sapiens] PFTA_HUMAN Protein farnesyltransferase alpha subunit (EC 2.5.1.-) (CAAX farnesyltraπsferase alpha subunit) (RAS proteins prenyltransferase alpha) (FTase-alpha). [Homo sapiens] PGHD_CANFA Prostaglandin-H2 D-lsomerase precursor (EC 5.3.99.2) (Lipocalln-type prostaglandin-D synthase)

(Glutathione-independent PGD synthetase) (Prostaglandin D2 synthase) (PGD2 synthase) (PGDS2)

(PGDS). [Canis familiaris] PGHD_MOUSE Prostaglandin-H2 D-isomerase precursor (EC 5.3.99.2) (Lipocalln-type prostaglandln-D synthase)

(Glutathione-independent PGD synthetase) (Prostaglandin-H2 D-isomerase) (PGD2 synthase) (PGDS2)

(PGDS). [Mus musculus] PGT1_HUMAN Geranyigeranyi transferase type I beta subunit (EC 2.5.1.-) (Type I protein geranyl-geranyltransferase beta subunit) (GGTase-I-beta). [Homo sapiens] PGT1_RAT Geranyigeranyi transferase type I beta subunit (EC 2.5.1.-) (Type I protein geranyl-geranyltransferase beta subunit) (GGTase-I-beta). [Rattus norvegicus] PGTA_HUMAN RAB geranyigeranyltransferase alpha subunit (EC 2.5.1.-) (RAB geranyi- geranyitransferase alpha subunit)

(RAB GG transferase alpha) (RAB GGTase alpha). [Homo sapiens] PHS1_HUMAN Glycogen phosphorylase, liver form (EC 2.4.1.1). [Homo sapiens] PHS2JHUMAN Glycogen phosphorylase, muscle form (EC 2.4.1.1) (Myophosphoryl PHS3JHUMAN Glycogen phosphorylase, brain form (EC 2.4.1.1). [Homo sapiens] PIB1_HUMAN l-phosphatldylinositol-4,5-bisphosphate phosphodiesterase beta PIB4JHUMAN l-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta 4 (EC 3.1.4.11) (Phosphoinositide phospholipase C) (PLC-beta-4) (Phospholipase C-beta-4). [Homo sapiens] PIB4_RAT l-phosphatldylinositol-4,5-bisphosphate phosphodiesterase beta 4 (EC 3.1.4.11) (Phosphoinositide phospholipase C) (PLC-beta-4) (Phospholipase C-beta-4). [Rattus norvegicus] PIG2JHUMAN l-phosphatidylinositol-4,5-b!sphosphate phosphodiesterase gamma 2 (EC 3.1.4.11) (Phosphoinositide phospholipase C) (PLC-gamma-2) (Phospholipase C-gamma-2) (PLC-IV). [Homo sapiens] PIN1_HUMAN Peptidyl-prolyl cis-trans isomerase NIMA-lnteracting 1 (EC 5.2.1.8) (Rotamase Pinl) (PPIase Pinl). [Homo sapiens] PIN4_HUMAN Peptidyl-prolyl cis-trans Isomerase NIMA-lnteracting 4 (EC 5.2.1.8) (Rotamase Pin4) (PPIase Pin4)

(Parvulin 14) (Parl4) (Peptidyl-prolyl cis/trans isomerase EPVH) (hParl4). [Homo sapiens] PIN4_MOUSE Peptidyl-prolyl cis-trans isomerase NIMA-lnteracting 4 (EC 5.2.1.8) (Rotamase Pin4) (PPIase Pln4). [Mus musculus] PLDl_MOUSE Phospholipase Dl (EC 3.1.4.4) (PLD 1) (Choline phosphatase 1) (Phosphatidylcholine-hydrolyzing phospholipase Dl) (mPLDl). [Mus musculus] PLΞB_HUMAN Glycerol-3-phosphate acyltransferase, mitochondrial precursor (EC 2.3.1.15) (GPAT). [Homo sapiens] PLSB_RAT Glycerol-3-phosphate acyltransferase, mitochondrial precursor (EC

PMG3_HUMAN Putative phosphoglycerate mutase 3 (EC 5.4.2.1) (EC 5.4.2.4) (EC 3.1.3.13). [Homo sapiens] PNPH HUMAN Purine nucleoside phosphorylase (EC 2.4.2.1) (Inosine phosphorylase) (PNP). [Homo sapiens] PON3_HUMAN Serum paraoxonase/arylesterase 3 (EC 3.1.1.2) (EC 3.1.8.1) (PON 3) (Serum aryldialkylphosphatase 3)

(A-esterase 3) (Aromatic esterase 3). [Homo sapiens] PP11 HUMAN Placental protein 11 precursor (EC 3.4.21.-) (PP11). [Homo sapiens] PPBN_HUMAN Alkaline phosphatase, placental-like precursor (EC 3.1.3.1) (Nagao isozyme) (Germ-cell alkaline phosphatase) (PLAP-like) (ALP-1). [Homo sapiens] PP03JHUMAN Poly [ADP-rlbose] polymerase-3 (EC 2.4.2.30) (PARP-3) (NAD(+) A PPOVJHUMAN Vault poly(ADP-ribose) polymerase (EC 2.4.2.30) (VPARP) (193-kDa vault protein) (PARP-related/Ialphal- related H5/proline-rich) (PH5P). [Homo sapiens] PPP6JHUMAN Serine/threonine protein phosphatase 6 (EC 3.1.3.16) (PP6). [Homo sapiens]

PPT1_HUMAN Palmitoyl-protein thioesterase 1 precursor (EC 3.1.2.22) (Palmitoyl- protein hydrolase 1). [Homo sapiens] PS7L_HUMAN Proteasome subunit alpha type 7-like (EC 3.4.25.1). [Homo sapiens] PSA1_HUMAN Proteasome subunit alpha type 1 (EC 3.4.25.1) (Proteasome component C2) (Macropain subunit C2)

(Multicatalytic endopeptidase complex subunit C2) (Proteasome nu chain) (30 kDa prosomal protein)

(PROS-30). [Homo sapiens] PSA4_HUMAN Proteasome subunit alpha type 4 (EC 3.4.25.1) (Proteasome component C9) (Macropain subunit C9)

(Multicatalytic endopeptidase complex subunit C9) (Proteasome subunit L). [Homo sapiens] PSA6_HUMAN Proteasome subunit alpha type 6 (EC 3.4.25.1) (Proteasome lota chain) (Macropain lota chain)

(Multicatalytic endopeptidase complex lota chain) (27 kDa prosomal protein) (PROS-27) (p27K). [Homo sapiens] PSA6_MOUSE Proteasome subunit alpha type 6 (EC 3.4.25.1) (Proteasome lota chain) (Macropain lota chain)

(Multicatalytic endopeptidase complex iota chain). [Mus musculus] PSA7_HUMAN Proteasome subunit alpha type 7 (EC 3.4.25.1) (Proteasome subunit RC6-1) (Proteasome subunit XAPC7).

[Homo sapiens] PSA_HUMAN Puromycin-sensitive aminopeptidase (EC 3.4.11.-) (PSA). [Homo sapiens]

PSA_MOUSE Puromycin-sensitive aminopeptidase (EC 3.4.11.-) (PSA). [Mus mus PSB3_MOUSE Proteasome subunit beta type 3 (EC 3.4.25.1) (Proteasome theta chain) (Proteasome chain 13)

(Proteasome component C10-II). [Mus musculus] PSBA_HUMAN Proteasome subunit beta type 10 precursor (EC 3.4.25.1) (Proteasome MECI-1) (Macropain subunit MECI-

1) (Multicatalytic endopeptidase complex subunit MECI-1). [Homo sapiens] PSBA_MOUSE Proteasome subunit beta type 10 precursor (EC 3.4.25.1) (Proteasome MECI-1) (Macropain subunit MECI-

1) (Multicatalytic endopeptidase complex subunit MECI-1). [Mus musculus] PTEI HUMAN Peroxisomal acyl-coenzyme A thloester hydrolase 1 (EC 3.1.2.2) (Peroxisomal long-chain acyl-coA thioesterase 1) (HIV-Nef associated acyl coA thioesterase) (Thioesterase II) (hTE). [Homo sapiens] PTNB_MOUSE Protein-tyrosine phosphatase, non-receptor type 11 (EC 3.1.3.48) (Proteln-tyrosine phosphatase SYP).

[Mus musculus] PTNE_HUMAN Protein tyrosine phosphatase, non-receptor type 14 (EC 3.1.3.48) (Protein-tyrosine phosphatase pez).

[Homo sapiens] PURIJHUMAN Amidophosphoribosyltransferase precursor (EC 2.4.2.14) (Glutamine phosphoribosylpyrophosphate amidotransferase) (ATASE) (GPAT). [Homo sapiens] PUR1_RAT Amidophosphoribosyltransferase precursor (EC 2.4.2.14) (Glutamine phosphoribosylpyrophosphate amidotransferase) (ATASE) (GPAT). [Rattus norvegicus] PUR2_HUMAN Trifunctional purine biosynthetic protein adenosine-3 [Includes: Phosphorlbosylamine— glycine ligase (EC

6.3.4.13) (GARS) (Glycinamlde ribonucleotide synthetase) (Phosphoribosylglycinamide synthetase);

Phosphoribosylformylglycinamidine cyclo-ligase (EC 6. PUR6_HUMAN Multifunctional protein ADE2 [Includes: Phosphoribosylaminoimidazole- succinocarboxamide synthase (EC

6.3.2.6) (SAICAR synthetase); Phosphoribosylaminoimidazole carboxylase (EC 4.1.1.21) (AIR carboxylase)

(AIRC)]. [Homo sapiens] PUR6_RAT Multifunctional protein ADE2 [Includes: Phosphoribosylaminoimidazole- succinocarboxamide synthase (EC

(AIRC)]. [Rattus norvegicus] PUR9_HUMAN Bifunctional purine biosynthesis protein PURH [Includes: Phosphoribosylaminolmidazolecarboxamide formyltransferase (EC 2.1.2.3) (AICAR transformylase); IMP cyclohydrolase (EC 3.5.4.10) (Inosinlcase)

(IMP synthetase) (ATIC)]. [Homo sapiens] PUR9_MOUSE Bifunctional purine biosynthesis protein PURH [Includes: Phosphoribosylaminoimidazolecarboxamide formyltransferase (EC 2.1.2.3) (AICAR transformylase); IMP cyclohydrolase (EC 3.5.4.10) (Inosinlcase)

(IMP synthetase) (ATIC)]. [Mus musculus] PYRGJHUMAN CTP synthase (EC 6.3.4.2) (UTP-ammonia ligase) (CTP synthetase). [Homo sapiens] Q29476 Phenol sulfotransferase (EC 2.8.2.1) (Aryl sulfotransferase) (Sulfo

Q8N7N8 Hypothetical protein FI-J40785. [Homo sapiens]

Q96LX4 Hypothetical protein FU33088. [Homo sapiens]

Q9DCY1 Peptldylprolyl Isomerase B (EC 5.2.1.8) (Peptidyl-prolyl cis-trans isomerase) (PPIase) (Rotamase). [Mus musculus] Q9TTC6 Cyclophilin 18 (EC 5.2.1.8) (Peptidyl-prolyl cis-trans isomerase) (PPIase) (Rotamase). [Oryctolagus cunicuius] RAG1_HUMAN V(D)J recombination activating protein 1 (RAG-1). [Homo sapiens] RBP2_HUMAN Ran-binding protein 2 (RanBP2) (Nuclear pore complex protein Nup358) (Nucleoporin Nup358) (358 kDa nucleoporln) (P270). [Homo sapiens] RELNJHUMAN Reelin precursor (EC 3.4.21.-). [Homo sapiens]

RENI_HUMAN Renin precursor, renal (EC 3.4.23.15) (Angiotenslnogenase). [Homo sapiens] RIB1_HUMAN Dolichyl-dlphosphooligosaccharlde~protein glycosyltransferase 67 kDa subunit precursor (EC 2.4.1.119)

(Ribophorin I) (RPN-I). [Homo sapiens] RIB2_HUMAN Dolichyl-diphosphoollgosaccharide~protelπ glycosyltransferase 63 kDa subunit precursor (EC 2.4.1.119)

(Ribophorin II) (RPN-II) (RIBIIR). [Homo sapiens] RISC_HUMAN Retlnoid-inducible serine carboxypeptidase precursor (EC 3.4.16.-) (Serine carboxypeptidase 1)

(MSTP034). [Homo sapiens] RNBP_HUMAN N-acylglucosamine 2-eplmerase (EC 5.1.3.8) (GlcNAc 2-eplmerase) (N-acetyl-D-glucosamine 2-eplmerase)

(Renin-blndlng protein) (RNBP). [Homo sapiens] RNBP_RAT N-acylglucosamiήe 2-epimerase (EC 5.1.3.8) (GlcNAc 2-epimerase) (N-acetyl-D-glucosamlne 2-epimerase)

(Renin-binding protein) (RNBP). [Rattus norvegicus] RNP6_HUMAN Ribonuclease 6 precursor (EC 3.1.27.-). [Homo sapiens]

RNP_MOUSE Ribonuclease pancreatic precursor (EC 3.1.27.5) (RNase 1) (RNase A). [Mus musculus] RNP_RATRT Ribonuclease pancreatic precursor (EC 3.1.27.5) (RNase 1) (RNase A). [Rattus rattus]

RPAl_MOUSE DNA-directed RNA polymerase I largest subunit (EC 2.7.7.6) (RNA polymerase 1 194 kDa subunit)

(RPA194). [Mus musculus] RR42JHUMAN Exosome complex exonuciease RRP42 (EC 3.1.13.-) (Ribosomal RNA processing protein 42) (p8). [Homo sapiens] RR44JHUMAN Exosome complex exonuciease RRP44 (EC 3.1.13.-) (Ribosomal RNA processing protein 44) (DIS3 protein homolog). [Homo sapiens] SAH2_HUMAN Putative adenosylhomocysteinase 2 (EC 3.3.1.1) (S-adenosyl-L- homocysteine hydrolase) (AdoHcyase).

[Homo sapiens] SAHH_HUMAN Adenosylhomocysteinase (EC 3.3.1.1) (S-adenosyl-L-homocysteine hydrolase) (AdoHcyase). [Homo sapiens] SCB2JHUMAN Succinyl-CoA ligase [GDP-forming] beta-chain, mitochondrial precursor (EC 6.2.1.4) (Succinyl-CoA synthetase, betaG chain) (SCS-betaG) (GTP- specific succinyl-CoA synthetase beta subunit) (Fragment).

[Homo sapiens] SCOT_HUMAN Succlnyl-CoA:3-ketoacld-coenzyme A transferase, mitochondrial precursor (EC 2.8.3.5) (Succinyi CoA:3- oxoacid CoA-transferase). [Homo sapiens] SDHL_RAT L-serine dehydratase/L-threonine deaminase [Includes: L-serlne dehydratase (EC 4.3.1.17) (L-serine deaminase) (SDH); L-threonine dehydratase (EC 4.3.1.19) (L-threonine deaminase) (TDH)]. [Rattus norvegicus] SEN1_HUMAN Sentrin-speciflc protease 1 (EC 3.4.22.-) (Sentrin/SUMO-specific protease SENP1). [Homo sapiens] SEN6_HUMAN Sentπn-specific protease 6 (EC 3.4.22.-) (Sentπn/SUMO-specific protease SENP6) (SUMO-1 specific protease 1) (Protease FKSG6). [Homo sapiens] SEN7_HUMAN Sentrin-specific protease 7 (EC 3.4.22.-) (Sentrin/SUMO-specific protease SENP7) (SUMO-1 specific protease 2). [Homo sapiens] SERCJ-iUMAN Phosphoserlne aminotransferase (EC 2.6.1.52) (PSAT). [Homo sapiens] SHH_HUMAN Sonic hedgehog protein precursor (SHH) (HHG-1). [Homo sapiens] SI4C_HUMAN CMP-N-acetylneuramlnate-beta-galactosamlde-alpha-2,3-slalyltransferase (EC 2.4.99.-) (Beta-galactoside alpha-2,3-sialyltransferase) (Alpha 2,3-sialyltransferase IV) (Alpha 2,3-ST) (Gal-NAc6S) (STZ) (SIAT4-C)

(ST3Gal III) (ΞAT-3) (ST-4). [Homo sapiens] SIA1_HUMAN CMP-N-acetylneuramιnate-beta-galactosamide-alpha-2,6-slalyltransferase (EC 2.4.99.1) (Beta-galactoside alpha-2,6-sialyltransferase) (Alpha 2,6-ST) (Sialyltransferase 1) (ST6Gal I) (B-cell antigen CD75). [Homo sapiens] SP25_HUMAN Microsomal signal peptidase 25 kDa subunit (EC 3.4.-.-) (SPase 25 kDa subunit) (SPC25). [Homo sapiens] SP25_MOUSE Microsomal signal peptidase 25 kDa subunit (EC 3.4.-.-) (SPase 25 kDa subunit) (SPC25). [Mus musculus] SPEEJHUMAN Spermidine synthase (EC 2.5.1.16) (Putresclne aminopropyltransferase) (SPDSY). [Homo sapiens] SRR_MOUSE Serine racemase (EC 5.1.1.-). [Mus musculus] STK1_RAT Sulfotransferase Kl (EC 2.8.2.-) (rSULTlC2). [Rattus norvegicus]

STK2_RAT Sulfotransferase K2 (EC 2.8.2.-) (rSULTlC2A). [Rattus norvegicus]

SUAC_RAT N-hydroxyarylamine sulfotransferase (EC 2.8.2.-) (HAST-I). [Rattus norvegicus]

SUAR_RAT Aryl sulfotransferase (EC 2.8.2.1) (Phenol sulfotransferase) (PST-1) (Sulfokinase) (Aryl sulfotransferase IV)

(ASTIV) (Tyrosine-ester sulfotransferase) (Minoxidil sulfotransferase). [Rattus norvegicus] SUDY_RAT DOPA/tyrosine sulfotransferase (EC 2.8.1.-). [Rattus norvegicus]

SUH3_RAT Alcohol sulfotransferase (EC 2.8.2.2) (Hydroxysteroid sulfotransferase) (ST) (ST-60). [Rattus norvegicus]

SUHS_RAT Alcohol sulfotransferase (EC 2.8.2.2) (Hydroxysteroid sulfotransferase) (ST) (ST-20). [Rattus norvegicus]

SU01_RAT Estrogen sulfotransferase, Isoform 1 (EC 2.8.2.4) (EST-1) (Sulfotransferase, estrogen-preferring) (Estrone sulfotransferase). [Rattus norvegicus] SUP1_HUMAN Phenol-sulfating phenol sulfotransferase 1 (EC 2.8.2.1) (P-PST) (Thermostable phenol sulfotransferase)

(Ts-PST) (HAST1/HAST2) (ST1A3). [Homo sapiens] SUPMJHUMAN Monoamine-sulfating phenol sulfotransferase (EC 2.8.2.1) (Sulfotransferase, monoamine-preferπng) (M-

PST) (Thermolabile phenol sulfotransferase) (TL-PST) (Placental estrogen sulfotransferase)

(Catecholamlne-sulfating phenol sulfotransferase) (HAST3). [Horn SUPP_BOVIN Phenol-sulfating phenol sulfotransferase (EC 2.8.2.1) (P-PST). [Bos taurus] SYJ1_B0VIN Synaptojanin 1 (EC 3.1.3.36) (Synaptic lnosιtol-l,4,5-trisphosphate 5- phosphatase 1) (pl50) (Fragment).

[Bos taurus] TAL1_HUMAN Transaldolase (EC 2.2.1.2). [Homo sapiens]

THEA_HUMAN Brown fat inducibie thioesterase (EC 3.1.2.-) (BFIT) (Adipose associated thioesterase). [Homo sapiens] THIK_HUMAN 3-ketoacyl-CoA thiolase, peroxisomal precursor (EC 2.3.1.16) (Beta- ketothiolase) (Acetyl-CoA acyltransferase) (Peroxisomal 3-oxoacyl- CoA thiolase). [Homo sapiens] THILJHUMAN Acetyl-CoA acetyltransferase, mitochondrial precursor (EC 2.3.1.9) (Acetoacetyl-CoA thiolase) (T2). [Homo sapiens] THIL_RAT Acetyl-CoA acetyltransferase, mitochondrial precursor (EC 2.3.1.9) (Acetoacetyl-CoA thiolase). [Rattus norvegicus] THIM_RAT 3-ketoacyl-CoA thiolase, mitochondrial (EC 2.3.1.16) (Beta- ketothiolase) (Acetyl-CoA acyltransferase)

(Mitochondrial 3-oxoacyl- CoA thiolase). [Rattus norvegicus] THRB_HUMAN Prothrombin precursor (EC 3.4.21.5) (Coagulation factor II). [Homo sapiens] THTR_RAT Thiosulfate sulfurtransferase (EC 2.8.1.1) (Rhodanese) (Fragment). [Rattus norvegicus]

TI60JHUMAN 60 kDa Tat interactive protein (Tlp60) (HIV-1 Tat interactive protein) (cPLA(2) interacting protein). [Homo sapiens] TKT2_HUMAN Transketolase-like 1 (EC 2.2.1.1) (Transketolase 2) (TK 2) (Tra TKT_HUMAN Transketolase (EC 2.2.1.1) (TK). [Homo sapiens] TKT_RAT Transketolase (EC 2.2.1.1) (TK). [Rattus norvegicus]

TP3BJHUMAN DNA topoisomerase III beta-1 (EC 5.99.1.2). [Homo sapiens] TPP1_RAT Tripeptldyl-peptidase I precursor (EC 3.4.14.9) (TPP-I) (Tripeptidyl aminopeptidase) (Lysosomal pepstatin insensitive protease) (LPIC). [Rattus norvegicus] TRFL_HUMAN Lactotransferrin precursor (Lactoferrin) [Contains: Lactoferroxin A; Lactoferroxin B; Lactoferroxin C],

[Homo sapiens] TRPC_ARATH Indole-3-glycerol phosphate synthase, chlo TRUA_HUMAN tRNA pseudourldine synthase A (EC 4.2.1.70) (Pseudourldylate synthase I) (Pseudourldine synthase I)

(Uracil hydrolyase). [Homo sapiens] TRY2_MOUSE Trypsin II, anlonlc precursor (EC 3.4.21.4) (Pretrypsinogen II). [Mus musculus] TRY3_RAT Trypsin III, catlonlc precursor (EC 3.4.21.4) (Pretrypsinogen III). [Rattus norvegicus]

UBA1_HUMAN Ubiquitin-activating enzyme El (A1S9 protein). [Homo sapiens] UBAl_MOUSE Ublqultln-activatlng enzyme El 1. [Mus musculus] UBC7_HUMAN Ublqultin-conjugatlng enzyme E2-18 kDa UbcH7 (EC 6.3.2.19) (Ubiquitin- protein ligase) (Ubiquitin carrier protein) (UbcM4) (E2-F1) (L-UBC). [Homo sapiens] UBCI_HUMAN Ubiquitin-like protein SUMO-1 conjugating enzyme (EC 6.3.2.19) (SUMO- 1-protein ligase) (Ubiquitin carrier protein) (Ublqultin-conjugatlng enzyme UbcE2A) (P18). [Homo sapiens] UBCNJHUMAN Ubiquitin-conjugating enzyme E2 N (EC 6.3.2.19) (Ubiquitin-proteln ligase N) (Ubiquitin carrier protein N)

(Ubcl3) (Bendless-like ubiquitin conjugating enzyme). [Homo sapiens] UBL1_HUMAN Ubiquitin carboxyl-terminal hydrolase isozyme LI (EC 3.4.19.12) (UCH- LI) (Ubiquitin thiolesterase LI)

(Neuron cytoplasmic protein 9.5) (PGP 9.5) (PGP9.5). [Homo sapiens] UBP5_HUMAN Ubiquitin carboxyl-terminal hydrolase 5 (EC 3.1.2.15) (Ubiquitin thiolesterase 5) (Ubiquitin-specific processing protease 5) (Deublquitlnating enzyme 5) (Isopeptidase T). [Homo sapiens] UBP7_HUMAN Ubiquitin carboxyl-terminal hydrolase 7 (EC 3.1.2.15) (Ubiquitin thiolesterase 7) (Ubiquitin-specific processing protease 7) (Deubiqultinating enzyme 7) (Herpesvirus associated ubiquitin-specific protease).

[Homo sapiens] UD13_RAT UDP-glUcuronosyltransferase 1-3 precursor, microsomal (EC 2.4.1.17) (UDPGT) (UGT1*3) (UGT1-03)

(UGT1.3) (UGT1A3) (B3). [Rattus norvegicus] UDB4JHUMAN UDP-glucuronosyltransferase 2B4 precursor, microsomal (EC 2.4.1.17) (UDPGT) (Hyodeoxychollc acid)

(HLUG25) (UDPGTH-1). [Homo sapiens] UDB6_RAT UDP-glucuronosyltransferase 2B6 precursor, microsomal (EC 2.4.1.17) (UDPGT) (17-beta-hydroxysteroid specific) (UDPGTR-5). [Rattus norvegicus] UDBC_RAT UDP-glucuronosyltransferase 2B12 precursor, microsomal (EC 2.4.1.17) (UDPGT). [Rattus norvegicus]

UGG2_HUMAN UDP-glucose:glycoprotein glucosyltransferase 2 precursor (EC 2.4.1.-) (UDP— Glc:glycoprotein glucosyltransferase 2) (UGT 2) (HUGT2). [Homo sapiens] VAG1_HUMAN Vacuolar ATP synthase subunit G 1 (EC 3.6.3.14) (V-ATPase G subunit 1) (Vacuolar proton pump G subunit

1) (V-ATPase 13 kDa subunit 1) (Vacuolar ATP synthase subunit M16). [Homo sapiens] VLCS_HUMAN Very-long-chain acyl-CoA synthetase (EC 6.2.1.-) (Very-long-chain- fatty-acld-CoA ligase). [Homo sapiens] VLCS_MOUSE Very-long-chain acyl-CoA synthetase (EC 6.2.1.-) (Very-long-chain- fatty-acid-CoA ligase). [Mus musculus] VLCS_RAT Very-long-chain acyl-CoA synthetase (EC 6.2.1.-) (Very-long-chain- fatty-acid-CoA ligase). [Rattus norvegicus] VNN1_HUMAN Pantetheinase precursor (EC 3.5.1.-) (Pantetheine hydrolase) (Vascular non-inflammatory molecule 1)

(Vanin 1) (Tiff66). [Homo sapiens] VNN2_HUMAN Vascular non-inflammatory molecule 2 precursor (Vanin 2) (Glycosylphosphatldyl inositol-anchored protein

GPI-80) (FOAP-4 protein). [Homo sapiens] Y153_HUMAN Hypothetical protein KIAA0153. [Homo sapiens] Y173_HUMAN Hypothetical protein KIAA0173. [Homo sapiens] Y934_HUMAN Hypothetical protein KIAA0934. [Homo sapiens]

Structural molecules

AAC1_HUMAN Alpha-actinin 1 (Alpha-actinln cytoskeletal Isoform) (Non-muscle alpha-actlnln 1) (F-actln cross linking protein). [Homo sapiens]

AAC3_HUMAN Alpha-actinin 3 (Alpha actinln skeletal muscle isoform 3) (F-actln cross linking protein). [Homo sapiens]

AA04JHUMAN Alpha-actlnln 4 (Non-muscle alpha-actinin 4) (F-actin cross linking protein). [Homo sapiens]

ACTA_HUMAN Actin, aortic smooth muscle (Alpha-actin 2). [Homo sapiens]

ACTB_CRIGR Actin, cytoplasmic 1 (Beta-actin). [Cricetulus grlseus]

ACTB_HUMAN Actin, cytoplasmic 1 (Beta-actin). [Homo sapiens]

ACTB_RABIT Actin, cytoplasmic 1 (Beta-actin). [Oryctolagus cunicuius]

ACTCJHUMAN Actin, alpha cardiac. [Homo sapiens]

ACTH_HUMAN Actin, gamma-enteric smooth muscle (Alpha-actin 3). [Homo sapiens]

ACTS_HUMAN Actin, alpha skeletal muscle (Alpha-actin 1). [Homo sapiens]

ANK2_HUMAN Ankyrin 2 (Brain ankyrin) (Ankyrin B) (Ankyrin, nonerythroid). [Homo sapiens]

AR16JHUMAN ARP2/3 complex 16 kDa subunit (P16-ARC) (Actin-related protein

AR1BJHUMAN ARP2/3 complex 41 kDa subunit (P41-ARC) (Actin-related protein 2/3 complex subunit IB). [Homo sapiens]

AR21JHUMAN ARP2/3 complex 21 kDa subunit (P21-ARC) (Actin-related protein 2/3 complex subunit 3). [Homo sapiens]

A 34JHUMAN ARP2/3 complex 34 kDa subunit (P34-ARC) (Actin-related protein 2/3 complex subunit 2). [Homo sapiens]

ARP2_HUMAN Actln-llke protein 2 (Actin-related protein 2). [Homo sapiens]

ARP3JHUMAN Actln-llke protein 3 (Actin-related protein 3) (Actin-2). [Homo sapiens]

B53A_HUMAN 53 kDa BRGl-associated factor A (Actin-related protein Baf53a) (ArpNbeta). [Homo sapiens]

BPEAJHUMAN Bullous pemphigoid antigen 1, isoforms 6/9/10 (Trabeculln-beta) (Bullous pemphigoid antigen) (BPA)

(Hemidesmosomal plaque protein) (Dystonia musculorum protein). [Homo sapiens]

CA11_M0USE Collagen alpha 1(1) chain precursor. [Mus musculus]

CA13_HUMAN Collagen alpha l(III) chain precursor. [Homo sapiens]

CA14JHUMAN Collagen alpha 1(IV) chain precursor. [Homo sapiens]

CA15_HUMAN Collagen alpha 1(V) chain precursor. [Homo sapiens]

CA16_HUMAN Collagen alpha 1(VI) chain precursor. [Homo sapiens]

CA18_MOUSE Collagen alpha l(VIII) chain precursor. [Mus musculus]

CA1A_HUMAN Collagen alpha 1(X) chain precursor. [Homo sapiens]

CAIBJHUMAN Collagen alpha 1(XI) chain precursor. [Homo sapiens]

CA1C_HUMAN Collagen alpha l(XII) chain precursor. [Homo sapiens]

CA1C_RAT Collagen alpha l(XII) chain (Fragment). [Rattus norvegicus]

CA1E_HUMAN Collagen alpha 1(XV) chain precursor. [Homo sapiens]

CA1F_HUMAN Collagen alpha l(XVI) chain precursor. [Homo sapiens]

CA21_MOUSE Collagen alpha 2(1) chain precursor. [Mus musculus]

CA24JHUMAN Collagen alpha 2(IV) chain precursor. [Homo sapiens]

CA2B_HUMAN Collagen alpha 2(XI) chain precursor. [Homo sapiens]

CA34JHUMAN Collagen alpha 3(IV) chain precursor (Goodpasture antigen). [Homo sapiens]

CA36_HUMAN Collagen alpha 3(VI) chain precursor. [Homo sapiens]

CCG4_HUMAN Voltage-dependent calcium channel gamma-4 subunit (Neuronal voltage- gated calcium channel gamma-4 subunit). [Homo sapiens]

CLH1_HUMAN Clathrin heavy chain 1 (CLH-17). [Homo sapiens]

C01A_HUMAN Coronin-like protein p57 (Coronln 1A). [Homo sapiens]

COMP_HUMAN Cartilage oligomeric matrix protein precursor (COMP). [Homo sapiens]

CRAA_HUMAN Alpha crystallin A chain. [Homo sapiens]

CTD1_HUMAN Catenin delta- 1 (pl20 catenin) (pl20(ctn)) (Cadherln-associated Src substrate) (CAS) (pl20(cas)). [Homo sapiens]

CTN1--HUMAN Alpha-1 catenin (Cadherln-associated protein) (Alpha E-catenin). [Homo sapiens]

DMD_CANFA Dystrophin. [Canis familiaris] DMD_ HUMAN Dystrophln. [Homo sapiens] E4L2_ HUMAN Band 4.1-like protein 2 (Generally expressed protein 4.1) (4 1G). [Homo sapiens] E4L2_ MOUSE Band 4.1-like protein 2 (Generally expressed protein 4.1) (4.1G). [Mus musculus] FBN2. HUMAN Fibrillin 2 precursor. [Homo sapiens] FINC_ HUMAN Flbronectin precursor (FN) (Cold-insoluble globulin) (CIG). [Homo sapiens] K1CJ. HUMAN Keratin, type I cytoskeletal 10 (Cytokeratin 10) (K10) (CK 10). [Homo sapiens] K1CS JHUMAN Keratin, type I cytoskeletal 19 (Cytokeratin 19) (K19) (CK 19). [Homo sapiens] K22E HUMAN Keratin, type II cytoskeletal 2 epidermal (Cytokeratin 2e) (K2e) (CK 2e). [Homo sapiens] K220 JHUMAN Keratin, type II cytoskeletal 2 oral (Cytokeratin 2P) (K2P) (CK 2P). [Homo sapiens] K2C1 HUMAN Keratin, type II cytoskeletal 1 (Cytokeratin 1) (Kl) (CK 1) (67 kDa cytokeratin) (Hair alpha protein).

[Homo sapiens]

K2C5_HUMAN Keratin, type II cytoskeletal 5 (Cytokeratin 5) (K5) (CK 5) (58 kDa cytokeratin). [Homo sapiens] K2C7 HUMAN Keratin, type II cytoskeletal 7 (Cytokeratin 7) (K7) (CK 7) (Sarcolectin). [Homo sapiens] K2C8_HUMAN Keratin, type II cytoskeletal 8 (Cytokeratin 8) (K8) (CK 8). [Homo sapiens] LAMA_HUMAN Lamm A/C (70 kDa lamin). [Homo sapiens] LMA1_HUMAN Laminin alpha-1 chain precursor (Laminin A chain). [Homo sapiens] LMA2_HUMAN Laminin alpha-2 chain precursor (Laminin M chain) (Merosin heavy chain). [Homo sapiens] LMA2_MOUSE Laminin alpha-2 chain precursor (Laminin M chain) (Merosin heavy chain). [Mus musculus] LMA3_HUMAN Laminin alpha-3 chain precursor (Eplligrin 170 kDa subunit) (E170) (Nicein alpha subunit). [Homo sapiens] LMA4_HUMAN Laminin alpha-4 chain precursor. [Homo sapiens] LMB1_HUMAN Laminin beta-1 chain precursor (Laminin BI chain). [Homo sapiens] LMB2_HUMAN Laminin beta-2 chain precursor (S-laminin) (Laminin Bis chain). [Homo sapiens] LMB3_HUMAN Laminin beta-3 chain precursor (Laminin 5 beta 3) (Laminin Blk LMG1JHUMAN Laminin gamma-1 chain precursor (Laminin B2 chain). [Homo sapiens] LMG1_M0USE Lammiπ gamma-1 chain precursor (Laminin B2 chain) [Mus musculus] MAT3_HUMAN Matrln 3. [Homo sapiens] MBP_HUMAN Myelin basic protein (MBP) (Myelm Al protein) (Myelln membrane encephalitogenic protein). [Homo sapiens]

MERL_HUMAN Merlin (Moesln-ezπn-radixin-like protein) (Schwannomm) (Schwannomerlin) (Neurofibromin 2). [Homo sapiens]

MLEY_HUMAN Myosin light chain 1, slow-twitch muscle A isoform (MLClsa) (Alkali). [Homo sapiens] MYM1_HUMAN Myomesin 1 (190 kDa titin-associated protein) (190 kDa connectln- associated protein). [Homo sapiens] MYPS_HUMAN Myosln-binding protein C, slow-type (Slow MyBP-C) (C-proteln, skeletal muscle slow-isoform). [Homo sapiens]

NEBL_HUMAN Nebulette (Actln-binding Z-disk protein). [Homo sapiens] NEBU_HUMAN Nebulm. [Homo sapiens] NHPX_.HU MAN NHP2-like protein 1 (High mobility group-like nuclear protein 2 homolog 1) ([U4/U6.U5] trl-snRNP 15.5 kDa protein) (OTK27) [Homo sapiens]

O18840 Beta-actin. [Cams familiaris]

PKP3_HUMAN Plakophllin 3. [Homo sapiens]

PLE1JHUMAN Plectin 1 (PLTN) (PCN) (Hemidesmosomal protein 1) (HD1). [Homo sapiens]

PLSI_HUMAN I-plastin (Intestine-specific plastin). [Homo sapiens]

PRLPJHUMAN Prolargln precursor (Prollne-arginine-rich end leucine-rich repeat protein). [Homo sapiens]

Q10465 Elastic titm (Fragment). [Homo sapiens]

Q13707 ACTA2 protein (Fragment). [Homo sapiens]

Q8SPX4 Beta-actin (Fragment). [Cams familiaris]

Q95164 Beta-actin (Fragment). [Cams familiaris]

R10A HU AN 60S πbosoma protein LlOa (CSA-19). [Homo sapiens]

R18A_HUMAN 28S πbosoma protein S18a, mitochondrial precursor (MRP-S18-a) (Mrpslδa) (MRP-S18-3). [Homo sapiens]

R261_HUMAN 60S πbosoma protein L26-like 1. [Homo sapiens]

R27A HUMAN 40S πbosoma protein S27a. [Homo sapiens]

R35A_HUMAN 60S πbosoma protein L35a. [Homo sapiens]

RADI_HUMAN Radixin. [Homo sapiens]

RL11_M0USE 60S ribosomal prote eiinr Lll. [Mus musculus]

RL12_HUMAN 60S ribosomal prote eiinr L12. [Homo sapiens]

RL12_MOUSE 60S ribosomal prote eiinr L12. [Mus musculus]

RL12_RAT 60S ribosomal prote εlinr L12. [Rattus norvegicus]

RL13_RAT 60S ribosomal prote siinr L13. [Rattus norvegicus]

RL17JHUMAN 60S ribosomal prote eiinr L17 (L23). [Homo sapiens]

RL19_HUMAN 60S ribosomal prote eiinr L19. [Homo sapiens]

RL1X_HUMAN 60S ribosomal prote -Iinr L18a. [Homo sapiens]

RL23_HUMAN 60S ribosomal prote .iini L23 (L17). [Homo sapiens]

RL24_HUMAN 60S ribosomal prote eiini L24 (L30). [Homo sapiens]

RL2A_RAT 60S ribosomal prote eiini L27a. [Rattus norvegicus]

RL2B_HUMAN 60S ribosomal prote eiini L23a. [Homo sapiens]

RL31_HUMAN 60S ribosomal prote eiini L31. [Homo sapiens]

RL4_HUMAN 60S ribosomal prote eiinr L4 (LI). [Homo sapiens]

RL4_RAT 60S ribosomal prote eiinr L4 (LI). [Rattus norvegicus]

RL5_HUMAN 60S ribosomal prote eiini L5. [Homo sapiens]

RL7_HUMAN 60S ribosomal prote eiinr L7. [Homo sapiens]

RL7_MOUSE 60S ribosomal prote eiini L7. [Mus musculus]

RL8JHUMAN 60S ribosomal prote eiinr L8. [Homo sapiens]

RL9_RAT 60S ribosomal prote eiinr L9. [Rattus norvegicus]

RLAO_HUMAN 60S acidic ribosoma all protein PO (L10E). [Homo sapiens]

RLA1_HUMAN 60S acidic ribosoma all protein PI. [Homo sapiens]

RLA2_HUMAN 60S acidic ribosoma all protein P2. [Homo sapiens]

RM13_HUMAN 60S ribosomal prote eilnr L13, mitochondrial (L13mt). [Homo sapiens]

RM39_HUMAN Mitochondrial 39s ribosomal protein L39 (L39mt) (MRP-L39) (MRP-L5) (PRED22 protein). [Homo sapiens]

RS10_HUMAN 40S ribosomal protein S10. [Homo sapiens] RS11JHUMAN 40S ribosomal protein Sll. [Homo sapiens]

RS12JHUMAN 40S ribosomal protein S12. [Homo sapiens]

RS14_HUMAN 40S ribosomal protein S14 (PRO2640). [Homo sapiens]

RS18_HUMAN 40S ribosomal protein S18 (KE-3) (KE3). [Homo sapiens]

RS19_RAT 40S ribosomal protein S19. [Rattus norvegicus]

RS21JHUMAN 40S ribosomal protein S21. [Homo sapiens]

RS21_MOUSE 40S ribosomal protein S21. [Mus musculus]

RS21_RAT 40S ribosomal protein S21. [Rattus norvegicus]

RS23_HUMAN 40S ribosomal protein S23. [Homo sapiens]

RS24_HUMAN 40S ribosomal protein S24 (S19). [Homo sapiens]

RS25_HUMAN 40S ribosomal protein S25. [Homo sapiens]

RS28_HUMAN 40S ribosomal protein S28. [Homo sapiens]

RS2JHUMAN 40S ribosomal protein S2 (S4) (LLREP3 protein). [Homo sapiens]

RS2_RAT 40S ribosomal protein S2. [Rattus norvegicus]

RS30_HUMAN 40S ribosomal protein S30. [Homo sapiens]

RS3_HUMAN 40S ribosomal protein S3. [Homo sapiens]

RS3_MOUSE 40S ribosomal protein S3. [Mus musculus]

RS5_HUMAN 40S ribosomal protein S5. [Homo sapiens]

RS5_MOUSE 40S ribosomal protein S5. [Mus musculus]

RS5_RAT 40S ribosomal protein S5. [Rattus norvegicus]

RS6_HUMAN 40S ribosomal protein S6 (Phosphoprotein NP33). [Homo sapiens]

RS7_HUMAN 40S ribosomal protein S7 (S8). [Homo sapiens]

RSP4_BOVIN 40S ribosomal protein P40 (CIO protein). [Bos taurus]

RSP4_MOUSE 40S ribosomal protein SA (P40) (34/67 kDa laminin receptor). [Mus musculus]

RSP4_RAT 40S ribosomal protein SA (P40) (34/67 kDa laminin receptor). [Rattus norvegicus]

SPCB_HUMAN Spectrin beta chain, erythrocyte (Beta-I spectrin). [Homo sapiens]

SPCN_HUMAN Spectrin alpha chain, brain (Spectrin, non-erythroid alpha chain) (Alpha-II spectrin) (Fodrin alpha chain).

[Homo sapiens]

SPCO_HUMAN Spectrin beta chain, brain 1 (Spectrin, non-erythroid beta chain 1) (Beta-II spectrin) (Fodrin beta chain).

[Homo sapiens]

SZ07_HUMAN Platelet basic protein precursor (PBP) (Small Inducibie cytokine B7) (CXCL7) [Contains: Connective-tissue activating peptide III (CTAP- III); Low-affinity platelet factor IV (LA-PF4); Beta-thromboglobulin (Beta-TG);

Neutrophil-activatlng peptide 2 (NAP-2)

TLN1_ HUMAN Talin 1. [Homo sapiens] TLN2_ HUMAN Talin 2. [Homo sapiens] TPM1_ .HUMAN Tropomyosln 1 alpha chain (Alpha-tropomyosin). [Homo sapiens] TPM2. HUMAN Tropomyosin beta chain (Tropomyosln 2) (Beta-tropomyosin). [Homo sapiens] TPM4_ HUMAN Tropomyosln alpha 4 chain (Tropomyosln 4) (TM30pl). [Homo sapiens] TSP1_ HUMAN Thrombospondin 1 precursor. [Homo sapiens] UTRO. HUMAN Utrophin (Dystrophin-related protein 1) (DRP1) (DRP). [Homo sapiens] VAPA_ HUMAN Vesicle-associated membrane protein-associated protein A (VAMP- associated protein A) (VAMP-A) (VAP-A)

(33 kDa Vamp-associated protein) (VAP-33). [Homo sapiens]

VAPA_MOUSE Vesicle-associated membrane protein-associated protein A (VAMP- associated protein A) (VAMP-A) (VAP-A)

(33 kDa Vamp-assoclated protein) (VAP-33). [Mus musculus]

VAPBJHUMAN Vesicle-associated membrane protein-associated protein B/C (VAMP- associated protein B/C) (VAMP-

B/VAMP-C) (VAP-B/VAP-C). [Homo sapiens]

VILL_HUMAN Villin-like protein. [Homo sapiens] VINC_HUMAN Vinculin (Metavinculin). Y256_HU AN Hypothetical protein KIAA0256 (Fragment). [Homo sapiens]

[00211] The invention illustratively described herein may be practiced in the absence of any element or elements, limitation or limitations which is not specifically disclosed herein. The terms and expressions which have been employed are used as terms of description and not of limitation, and there is no intention that in the use of such terms and expressions of excluding any equivalents of the features shown and described or portions thereof, but it is recognized that various modifications are possible within the scope of the invention claimed. Thus, it should be understood that although the present invention has been specifically disclosed by preferred embodiments and optional features, modification and variation of the concepts herein disclosed may be resorted to by those skilled in the art, and that such modifications and variations are considered to be within the scope of this invention as defined by the appended claims.

[00212] The contents of the articles, patents, and patent applications, and all other documents and electronically available information mentioned or cited herein, are hereby incoφorated by reference in their entirety to the same extent as if each individual publication was specifically and individually indicated to be incoφorated by reference. Applicants reserve the right to physically incoφorate into this application any and all materials and information from any such articles, patents, patent applications, or other documents.

[00213] The inventions illustratively described herein may suitably be practiced in the absence of any element or elements, limitation or limitations, not specifically disclosed herein. Thus, for example, the terms "comprising", "including," containing", etc. shall be read expansively and without limitation. Additionally, the terms and expressions employed herein have been used as terms of description and not of limitation, and there is no intention in the use of such terms and expressions of excluding any equivalents of the features shown and described or portions thereof, but it is recognized that various modifications are possible within the scope of the invention claimed. Thus, it should be understood that although the present invention has been specifically disclosed by preferred embodiments and optional features, modification and variation of the inventions embodied therein herein disclosed may be resorted to by those skilled in the art, and that such modifications and variations are considered to be within the scope of this invention.

[00214] The invention has been described broadly and genetically herein. Each of the narrower species and subgeneric groupings falling within the generic disclosure also form part of the invention. This includes the generic description of the invention with a proviso or negative limitation removing any subject matter from the genus, regardless of whether or not the excised material is specifically recited herein.

[00215] In addition, where features or aspects of the invention are described in terms of Markush groups, those skilled in the art will recognize that the invention is also thereby described in terms of any individual member or subgroup of members of the Markush group.

[00216] Other embodiments are set forth within the following claims.

Claims

We claim:

1. An acyl-nucleotide probe having the formula:

wherein

BASE is a 5- or 6-membered unsaturated heterocyclic ring comprising from 1 to 3 ring nitrogens, wherein the 5- or 6-membered unsaturated heterocyclic ring is covalently attached through a ring nitrogen to the 1' position of the ribose or deoxy-ribose, wherein the 5- or 6-membered unsaturated heterocyclic ring optionally comprises a 6-membered unsaturated carbocyclic or heterocyclic ring fused thereto, said fused ring comprising from 1 to 2 ring nitrogens, and wherein each carbon position in the BASE may be optionally substituted by a substituent independently selected from the group consisting of -H, -F, -Br, -CI, -SCH₃, -C(O)N(R)(R), -CN, -NO₂, -N(R)(R), =0, acetoxy, -C(R)(R)(R), -OCH₃, - OCH CH₃, methylene dioxy, trihalomethyl, trihalomethoxy, or -(CH₂)_mOH; R₂' and R₃> are independently selected from the group consisting of -H, -OH, -F, -Br, -CI, - SCH₃, -C(O)N(R)(R), -CN, -NO_, -N(R)(R), acetoxy, -C(R)(R)(R), -OCH₃, -OCH₂CH₃, methylene dioxy, trihalomethyl, trihalomethoxy, -(CH₂)_mOH, or -(CH₂)_m-phenyl where phenyl is optionally substituted with -F, -Br, -CI, -SCH₃, -C(O)N(R)(R), -CN, -NO₂, - N(R)(R), acetoxy, -C(R)(R)(R), -OCH₃, -OCH₂CH₃, methylene dioxy, trihalomethyl, trihalomethoxy, -(CH₂)_mOH; n is 0-2; m is 0 to 6;

TAG is a detectable label; each Z is independently O, S, NH, or methylene;

L is an optionally present alkyl or heteroalkyl group of 1-40 backbone atoms selected from the group consisting of -N(R)-, -O-, -S- or -C(R)(R)-, wherein said alkyl or heteroalkyl group optionally includes a carbocyclic or heterocyclic group; each R is independently H or -Cι-₆ alkyl straight or branched chain, or optionally form an optionally substituted fused carbocyclic or heterocyclic ring structure; and the carbonyl adjacent to L is bound to a carbon to form an acyl group; or a pharmaceutically acceptable salt or complex thereof.

2. An acyl-nucleotide probe according to claim 1, wherein BASE is a purine.

3. An acyl-nucleotide probe according to claim 1, wherein BASE is a pyrimidine.

4. An acyl-nucleotide probe according to claim 1, wherein BASE is selected from the group consisting of adenine, thymine, uracil, guanine, cytosine, inosine, 5- bromouracil, 5-fluorouracil, 2-aminopurine, N -cyclohexyl adenine, 8-azaguanine, and 5-fluorocytosine.

5. An acyl-nucleotide probe according to claim 4, wherein BASE is selected from the group consisting of adenine, thymine, uracil, guanine, and cytosine.

6. An acyl-nucleotide probe according to claim 1 , wherein R₂< and R₃- are independently H or OH.

7. An acyl-nucleotide probe according to claim 1, wherein R₂> and Ry are each OH.

8. An acyl-nucleotide probe according to claim 1, wherein L has the structure:

where x and y are independently in the range of 0 to 4, and X is O or CH₂. An acyl-nucleotide probe according to claim 1, wherein L has the structure:

O

^■NH(CH₂)₀-₄(CH₂CH₂)o-₄NHC(CH₂)₂-₁₀- - ; or

O O

-NH(CH₂)₀-₄(CH₂CH₂)o-₄NHC(CH₂)₂-₁₀C-

10. An acyl-nucleotide probe according to claim 8, wherein L has the structure - NHCCH.HOCHzCH^_M-.

11. An acyl-nucleotide probe according to claim 1 , wherein L comprises a triazole moiety.

12. An acyl nucleotide probe according to claim 1, wherein L comprises the following moiety:

13. An acyl-nucleotide probe according to claim 1, wherein the TAG is selected from the group consisting of:

wherein 5-substituted carboxyrhodamine or 5-substituted carboxyfluorescein may be replaced with 6-carboxyrhodamine or 6-carboxyfluorescein, or with a mixture of 5- and 6- substituted carboxyrhodamine or carboxyfluorescein. 14. An acyl-nucleotide probe having the structure:

wherein

BASE is a 5- or 6-membered unsaturated heterocyclic ring comprising from 1 to 3 ring nitrogens, wherein the 5- or 6-membered unsaturated heterocyclic ring is covalently attached through a ring nitrogen to the 1' position of the ribose or deoxy-ribose, wherein the 5- or 6-membered unsaturated heterocyclic ring optionally comprises a 6-membered unsaturated carbocyclic or heterocyclic ring fused thereto, said fused ring comprising from 1 to 2 ring nitrogens, and wherein each carbon position in the BASE may be optionally substituted by a substituent independently selected from the group consisting of -H, -F, -Br, -CI, -SCH₃, -C(O)N(R)(R), -CN, -NO₂, -N(R)(R), =O, acetoxy, -C(R)(R)(R), -OCH₃, - OCH CH₃, methylene dioxy, trihalomethyl, trihalomethoxy, or -(CH₂)_mOH; one of R₂> and Ry and Ry has the following structure:

and the other two of Rγ and R_3' and R₅> are independently selected from the group consisting of -H, -OH, -F, -Br, -CI, -SCH₃, -C(O)N(R)(R), -CN, -NO₂, -N(R)(R), acetoxy, -

C(R)(R)(R), -OCH₃, -OCH₂CH₃, methylene dioxy, trihalomethyl, trihalomethoxy, -

(CH )_mOH, or -(CH )_m-phenyl where phenyl is optionally substituted with -F, -Br, -CI, -

SCH₃, -C(O)N(R)(R), -CN, -NO₂, -N(R)(R), acetoxy, -C(R)(R)(R), -OCH₃, -OCH₂CH₃, methylene dioxy, trihalomethyl, trihalomethoxy, -(CH )_mOH; n is 0-2; m is 0 to 6;

TAG is a detectable label; each Z is independently O, S, NH, or methylene;

L is an optionally present alkyl or heteroalkyl group of 1-40 backbone atoms selected from the group consisting of -N(R)-, -O-, -S- or -C(R)(R)-, wherein said alkyl or heteroalkyl group optionally includes a carbocyclic or heterocyclic group; each R is independently H or -Cι-₆ alkyl straight or branched chain, or optionally form an optionally substituted fused carbocyclic or heterocyclic ring structure; and the carbonyl adjacent to L is bound to a carbon to form an acyl group; or a phannaceutically acceptable salt or complex thereof.

15. An acyl-nucleotide probe having the structure:

wherein n is 1-4; and

TAG is a detectable label; or a pharmaceutically acceptable salt or complex thereof.

16. An acyl-nucleotide probe having the structure:

wherein n is 1-4; and

17. An acyl-nucleotide probe having the structure:

wherein n is 1-4; and

TAG is a detectable label; or a phannaceutically acceptable salt or complex thereof.

18. An acyl-nucleotide probe having the structure:

wherein n is 1-4; and

19. An acyl-nucleotide probe having the structure:

wherein n is 1-4; and

20. A method for detennining the enzyme profile of one or more target proteins in a complex protein mixture, employing one or more probes comprising a nucleotide covalently bound tlirough the terminal phosphate of a 5' mono- di- or tri-phosphate to an acyl group, which is further covalently bound to a TAG via a linker moiety "L", wherein said acyl group forms an adduct with said target protein(s) when said probe is bound to said target protein(s), said method comprising: combining in a reaction medium said probe(s) and said complex protein mixture under conditions of reaction of said probe(s) with said nucleotide binding protein(s), whereby a conjugate of said probe(s) and said target protein(s) is formed; and determining said enzyme profile by generating a signal from one or more conjugates fonned thereby; wherein said probe(s) are selected from the nucleotide binding protein-directed probes of one of claims 1-18.

21. A method according to Claim 20, wherein said probe binds to a plurality of target proteins.

22. A composition comprising a purified labeled polypeptide having the structure:

O

TAG L C Polypeptide

wherein TAG is a detectable label or a solid support;

L is an optionally present alkyl or heteroalkyl group of 1-40 backbone atoms selected from the group consisting of -N(R)-, -O-, -S- or -C(R)(R)-, wherein said alkyl or heteroalkyl optionally includes a carbocyclic or heterocyclic group; the carbonyl adjacent to L is bound to a carbon to form an acyl group; and the acyl group is covalently attached through an amide, ester, or thioester linkage to a

Polypeptide amino acid residue.

23. A composition according to claim 22, wherein L has the structure:

where x and y are independently in the range of 0 to 4, and X is O or CH . 24. A composition according to claim 22, wherein L has the structure:

H H,

O

^■NH(CH )n- (CH₂CH₂)o-₄NHC(CH₂)2-ιo" " or

O O

^■ H(CH2)o-₄(CH₂CH₂)o- HC(CH₂)₂-ιo "

25. A composition according to claim 20, wherein L has the structure -

NH(CH₂)₂(OCH₂CH₂)₁-₄-.

26. A composition according to claim 20, wherein the TAG is selected form the group consisting of:

wherein 5-substituted carboxyrhodamine or 5-substituted carboxyfluorescein may be replaced with 6-carboxyrhodamine or 6-carboxyfluorescein, or with a mixture of 5- and 6- substituted carboxyrhodamine or carboxyfluorescein. 27. A tagged acyl phosphate or phosphonate probe having the formula:

O O

TAG- -L- -X

O-

wherein

X is an affinity moiety for directing the binding of said TAPP to one or more target proteins linked to the phophate through an oxygen or carbon;

TAG is a detectable label; L is an optionally present alkyl or heteroalkyl group of 1-40 backbone atoms selected from the group consisting of -N(R)-, -O-, -S- or -C(R)(R)-, wherein said alkyl or heteroalkyl group optionally includes a carbocyclic or heterocyclic group; each R is independently H or -Cι-₆ alkyl straight or branched chain, or optionally form an optionally substituted fused carbocyclic or heterocyclic ring structure; and the carbonyl adjacent to L is bound to a carbon to form an acyl group; or a pharmaceutically acceptable salt or complex thereof.

28. The tagged acyl phosphate probe of claim 27, wherein X is selected from the group consisting of a nucleotide, nucleotide analogue, optionally substituted naphthyl group, small molecule, steroid, peptide hormone, enzyme cofactor, vitamin, enzyme substrate, lipid, prostaglandin, or receptor ligand.

29. A method of synthesizing a tagged acyl phosphate or phosphonate probe, comprising: contacting a detectable label comprising a linking group L tenninating in a carboxyl group, with a nucleotide or nucleotide analogue comprising a 5 '-linked phosphate comprising an available -OH group in the presence of diisopropylcarbodiimide or isobutyl chloroformate and triethylamine to form said tagged acyl phosphate or phosphonate probe; and purifying said probe.