WO2004088325A3 - Determination of proteins and/or other molecules using mass spectroscopy - Google Patents

Determination of proteins and/or other molecules using mass spectroscopy Download PDF

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Publication number
WO2004088325A3
WO2004088325A3 PCT/US2004/009376 US2004009376W WO2004088325A3 WO 2004088325 A3 WO2004088325 A3 WO 2004088325A3 US 2004009376 W US2004009376 W US 2004009376W WO 2004088325 A3 WO2004088325 A3 WO 2004088325A3
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WO
WIPO (PCT)
Prior art keywords
proteins
species
substrate
self
cases
Prior art date
Application number
PCT/US2004/009376
Other languages
French (fr)
Other versions
WO2004088325A2 (en
Inventor
Marc W Kirschner
Judith A Jebanathirajah
Muhammad N Yousaf
Original Assignee
Harvard College
Marc W Kirschner
Judith A Jebanathirajah
Muhammad N Yousaf
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Harvard College, Marc W Kirschner, Judith A Jebanathirajah, Muhammad N Yousaf filed Critical Harvard College
Priority to CA002520191A priority Critical patent/CA2520191A1/en
Priority to US10/550,826 priority patent/US20070249060A1/en
Priority to EP04758432A priority patent/EP1606629A2/en
Priority to AU2004225496A priority patent/AU2004225496A1/en
Priority to JP2006509366A priority patent/JP2006521567A/en
Publication of WO2004088325A2 publication Critical patent/WO2004088325A2/en
Publication of WO2004088325A3 publication Critical patent/WO2004088325A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G01N33/6851—Methods of protein analysis involving laser desorption ionisation mass spectrometry
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/02—Details
    • H01J49/04—Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components
    • H01J49/0409—Sample holders or containers
    • H01J49/0418—Sample holders or containers for laser desorption, e.g. matrix-assisted laser desorption/ionisation [MALDI] plates or surface enhanced laser desorption/ionisation [SELDI] plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2610/00—Assays involving self-assembled monolayers [SAMs]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/24—Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Nanotechnology (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Optics & Photonics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Condensed Matter Physics & Semiconductors (AREA)
  • Materials Engineering (AREA)
  • Composite Materials (AREA)
  • General Chemical & Material Sciences (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Peptides Or Proteins (AREA)

Abstract

This invention generally relates to the determination of species such as proteins and/or small molecules on self-assembled monolayers using mass spectrometry. In some cases, the proteins and/or small molecules may be arranged on a substrate in an array, for example, in a microarray. In one set of embodiments, the invention relates to methods for determining proteins and/or small molecules bound to self-assembled monolayers using mass spectroscopy techniques such as MALDI and MALDI TOF techniques. This combination allows, for example, the systematic identification of unknown proteins from cell lysates. Identification of novel interactions can be achieved, in some cases, in instances where the binding partner to a particular target species is unknown. In another set of embodiments, the invention relates to methods of attaching a species to a self-assembled monolayer on a substrate such that the substrate can be used in a mass spectrometer, in some cases without requiring additional exposure of the substrate to water. For example, a target species may be detected and/or analysed using mass spectrometry in the presence of similar or “contaminating” species, without first removing the contaminating species.
PCT/US2004/009376 2003-03-26 2004-03-26 Determination of proteins and/or other molecules using mass spectroscopy WO2004088325A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CA002520191A CA2520191A1 (en) 2003-03-26 2004-03-26 Determination of proteins and/or other molecules using mass spectroscopy
US10/550,826 US20070249060A1 (en) 2003-03-26 2004-03-26 Determination of Proteins and/or Other Molecules Using Mass Spectroscopy
EP04758432A EP1606629A2 (en) 2003-03-26 2004-03-26 Determination of proteins and/or other molecules using mass spectroscopy
AU2004225496A AU2004225496A1 (en) 2003-03-26 2004-03-26 Determination of proteins and/or other molecules using mass spectroscopy
JP2006509366A JP2006521567A (en) 2003-03-26 2004-03-26 Measurement of proteins and / or other molecules using mass spectrometry

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US45810503P 2003-03-26 2003-03-26
US60/458,105 2003-03-26

Publications (2)

Publication Number Publication Date
WO2004088325A2 WO2004088325A2 (en) 2004-10-14
WO2004088325A3 true WO2004088325A3 (en) 2005-03-24

Family

ID=33131754

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/009376 WO2004088325A2 (en) 2003-03-26 2004-03-26 Determination of proteins and/or other molecules using mass spectroscopy

Country Status (6)

Country Link
US (1) US20070249060A1 (en)
EP (1) EP1606629A2 (en)
JP (1) JP2006521567A (en)
AU (1) AU2004225496A1 (en)
CA (1) CA2520191A1 (en)
WO (1) WO2004088325A2 (en)

Families Citing this family (16)

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Publication number Priority date Publication date Assignee Title
EP1654346B1 (en) 2003-06-12 2017-08-02 Accupath Diagnostic Laboratories, Inc. Method and system for the analysis of high density cells samples
US8294090B1 (en) * 2004-10-29 2012-10-23 Japan Science And Technology Agency Substrate for MALDI-TOF MS and mass spectrometry method using the same
JP2006170857A (en) * 2004-12-16 2006-06-29 Toyo Kohan Co Ltd Method for mass spectrometry of biomolecule on solid support and solid support therefor
JP2008547005A (en) * 2005-06-16 2008-12-25 スリーエム イノベイティブ プロパティズ カンパニー Method for classifying chemically cross-linked cell samples using mass spectra
JPWO2007145228A1 (en) * 2006-06-13 2009-11-05 独立行政法人産業技術総合研究所 Microarray fabrication method
KR100963539B1 (en) * 2007-12-11 2010-06-15 한국표준과학연구원 Evaluation Method of Organic or Bio- Conjugation on Nanoparticles Using Imaging of Time-of-Flight Secondary Ion Mass Spectrometry
JP4911415B2 (en) * 2008-03-28 2012-04-04 独立行政法人産業技術総合研究所 Non-specific adsorption suppression material
US9523680B2 (en) * 2010-06-30 2016-12-20 Ambergen, Inc. Global Proteomic screening of random bead arrays using mass spectrometry imaging
GB201110272D0 (en) 2011-06-17 2011-08-03 Isis Innovation Metod of detection by mass spectrometry
US11181448B2 (en) 2012-11-06 2021-11-23 Biodot, Inc. Controlled printing of a cell sample for karyotyping
WO2014197991A1 (en) 2013-06-12 2014-12-18 Muhammad Yousaf Compounds for promoting liposomal and cellular adhesion and compositions and methods of use thereof
KR101675021B1 (en) * 2014-11-12 2016-11-10 가천대학교 산학협력단 Method and apparatus for producing micro-arrray substrate
US9968683B2 (en) 2014-12-17 2018-05-15 Organolinx Corp. Method for conjugating molecules
WO2019232512A1 (en) * 2018-06-01 2019-12-05 Musc Foundatiion For Research Development Glycan analysis of proteins and cells
CN111089892A (en) * 2019-12-31 2020-05-01 北京毅新博创生物科技有限公司 Detection product for detecting A1 and A2 type β casein in dairy products by mass spectrometry
WO2024062974A1 (en) * 2022-09-22 2024-03-28 日産化学株式会社 Remover composition for light irradiation removal, multilayer body, and method for producing processed semiconductor substrate

Citations (2)

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WO2004005918A2 (en) * 2002-07-05 2004-01-15 University Of Chicago Characterization of biochips containing self-assembled monolayers with maldi-tof-ms

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US5620850A (en) * 1994-09-26 1997-04-15 President And Fellows Of Harvard College Molecular recognition at surfaces derivatized with self-assembled monolayers
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Publication number Priority date Publication date Assignee Title
WO2002006834A2 (en) * 2000-07-19 2002-01-24 Pointilliste, Inc. Nested sorting and high throughput screening
WO2004005918A2 (en) * 2002-07-05 2004-01-15 University Of Chicago Characterization of biochips containing self-assembled monolayers with maldi-tof-ms

Non-Patent Citations (4)

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Title
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Also Published As

Publication number Publication date
EP1606629A2 (en) 2005-12-21
JP2006521567A (en) 2006-09-21
US20070249060A1 (en) 2007-10-25
AU2004225496A1 (en) 2004-10-14
WO2004088325A2 (en) 2004-10-14
CA2520191A1 (en) 2004-10-14

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