WO2004079342A3 - Use of nucleic acid mimics for internal reference and calibration in a flow cell microarray binding assay - Google Patents

Use of nucleic acid mimics for internal reference and calibration in a flow cell microarray binding assay Download PDF

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Publication number
WO2004079342A3
WO2004079342A3 PCT/US2004/006479 US2004006479W WO2004079342A3 WO 2004079342 A3 WO2004079342 A3 WO 2004079342A3 US 2004006479 W US2004006479 W US 2004006479W WO 2004079342 A3 WO2004079342 A3 WO 2004079342A3
Authority
WO
WIPO (PCT)
Prior art keywords
flow cell
cartridge
binding assay
reagent
stream
Prior art date
Application number
PCT/US2004/006479
Other languages
French (fr)
Other versions
WO2004079342A2 (en
Inventor
Jeremy Lambert
Original Assignee
Hts Biosystems Inc
Jeremy Lambert
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to US45146803P priority Critical
Priority to US60/451,468 priority
Application filed by Hts Biosystems Inc, Jeremy Lambert filed Critical Hts Biosystems Inc
Priority claimed from US10/548,229 external-priority patent/US20060210984A1/en
Publication of WO2004079342A2 publication Critical patent/WO2004079342A2/en
Publication of WO2004079342A3 publication Critical patent/WO2004079342A3/en

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

Abstract

The present application describes a method for normalizing for variations in signal intensity observed in a biomolecular binding assay carried out in a flow cell cartridge. Variations in signal intensity occur as a result of the effect of the surfaces of a flow cell cartridge on the laminar flow of reagent through the cartridge. In any individual reagent stream, fluid flows faster in the center of the stream and slower at the outer periphery of the stream due to contact of the reagent with the walls of the cartridge, creating a parabolic fluid flow profile. The present invention describes a method for normalizing or calibrating out the differences in intensity observed in different regions of interest on a single chip or similar reactions carried out in different cartridges, as a result of these differential fluid flow rates. Microarray chips having integrated calibration regions are also described.
PCT/US2004/006479 2003-03-03 2004-03-03 Use of nucleic acid mimics for internal reference and calibration in a flow cell microarray binding assay WO2004079342A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US45146803P true 2003-03-03 2003-03-03
US60/451,468 2003-03-03

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US10/548,229 US20060210984A1 (en) 2003-03-03 2004-03-03 Use of nucleic acid mimics for internal reference and calibration in a flow cell microarray binding assay

Publications (2)

Publication Number Publication Date
WO2004079342A2 WO2004079342A2 (en) 2004-09-16
WO2004079342A3 true WO2004079342A3 (en) 2005-08-11

Family

ID=32962591

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/006479 WO2004079342A2 (en) 2003-03-03 2004-03-03 Use of nucleic acid mimics for internal reference and calibration in a flow cell microarray binding assay

Country Status (1)

Country Link
WO (1) WO2004079342A2 (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6251601B1 (en) * 1999-02-02 2001-06-26 Vysis, Inc. Simultaneous measurement of gene expression and genomic abnormalities using nucleic acid microarrays
US6281004B1 (en) * 1999-04-14 2001-08-28 Cytologix Corporation Quality control for cytochemical assays
US6362004B1 (en) * 1999-11-09 2002-03-26 Packard Biochip Technologies, Llc Apparatus and method for using fiducial marks on a microarray substrate

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6251601B1 (en) * 1999-02-02 2001-06-26 Vysis, Inc. Simultaneous measurement of gene expression and genomic abnormalities using nucleic acid microarrays
US6281004B1 (en) * 1999-04-14 2001-08-28 Cytologix Corporation Quality control for cytochemical assays
US6362004B1 (en) * 1999-11-09 2002-03-26 Packard Biochip Technologies, Llc Apparatus and method for using fiducial marks on a microarray substrate

Also Published As

Publication number Publication date
WO2004079342A2 (en) 2004-09-16

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