WO2004078750A1

WO2004078750A1 - Tetrahydropyran compounds as tachykinin antagonists

Info

Publication number: WO2004078750A1
Application number: PCT/GB2004/000918
Authority: WO
Inventors: Olivier Dirat; Jason Matthew Elliott; Ian Thomas Huscroft; Richard Alexander Jelley; Janusz Jozef Kulagowski; Piotr Antoni Raubo; Duncan Edward Shaw; Francine Sternfeld; Christopher John Swain
Original assignee: Merck Sharp & Dohme Limited
Priority date: 2003-03-07
Filing date: 2004-03-04
Publication date: 2004-09-16
Also published as: JP2006519822A; AU2004218228A1; EP1603901B1; US7393858B2; DE602004017141D1; ATE411311T1; EP1603901A1; CA2517422A1; US20060172999A1

Abstract

The present invention relates to the compounds of the formula (I), wherein R?1, R2, R3, R4, R5, R6, R7, R8, R9¿ and W are as defined herein, and pharmaceutically acceptable salts thereof; the compounds are of particular use in the treatment or prevention of depression, anxiety, pain, inflammation, migraine, emesis or postherpetic neuralgia.

Description

TETRAHYDROPYRAN COMPOUNDS AS TACHYKININ ANTAGONISTS

This invention relates to a class of tetrahydropyran compounds which are useful as tachykinin antagonists. More particularly, the compounds of the invention are useful as neurokinin 1 (NK-1) receptor antagonists.

The present invention provides compounds of the formula (I):

and pharmaceutically acceptable salts thereof wherein

W represents:

R¹ is hydrogen, halogen, C_1-όalkyl, C_1-6alkoxy, fluoro -galkyl, fluoroC_walkoxy, C_3-7cycloalkyl, C_3-7cycloalkylC_1-4alkyl, NO₂, CN, SR^a, SOR^a, SO₂R^a, CO₂R^a, CONR^aR^b, C_2-6alkenyl, C_2-6alkynyl or C_1-4alkyl substituted by C_1- alkoxy, wherein R^a and R^b each independently represent hydrogen or C_1- alkyl;

R² is hydrogen, halogen, C_1-6alkyl, fluoro -ealkyl or

substituted by C_1-4alkoxy;

R³ is hydrogen, halogen or fluoroC_1-6alkyl;

R⁴ is hydrogen, halogen, C_1-6alkyl, C_1-6alkoxy, fluoroC_1-6alkyl, fluoroC_1-6alkoxy, hydroxy, NO₂, CN, SR^a, SOR^a, SO₂R^a, CO₂R^a, CONR^aR^b, C _-6alkenyl, C_2-6alkynyl or C_1-4alkyl substituted by C_1-4alkoxy, wherein R^a and R are as previously defined;

R⁵ is hydrogen, halogen, C_1-6alkyl, fluoroC_1-6alkyl or C_1-6alkoxy substituted by C_1-4alkoxy;

R⁶ represents hydrogen or a Cι_-4alkyl group optionally substituted by a hydroxy group;

R represents hydrogen, ^all-vl, fluoroC_1-6alkyl, hydroxy, C_1-6alkoxy or hydroxyC _{\ -6}alkyl ;

R and R each independently represent hydrogen, halogen, C_1-6alkyl, CH₂OR^c, oxo, CO₂R^a or CONR^aR where R^a and R^b are as previously defined and R^c represents hydrogen, C_1-6alkyl or phenyl; and pharmaceutically acceptable salts thereof; wherein when W is

A represents an oxygen atom or a CH₂ group; B represents an oxygen atom or a CH₂ group, with the proviso that when A is an oxygen atom, B is a CH₂ group, and when A is a CH₂ group, B is an oxygen atom; n is 1 or 2; m is 1, 2 or 3, with the proviso that the sum total of m + n is 2, 3 or 4;

R¹⁰ is hydrogen, halogen, hydroxy, C_1- alkyl, C_3- cycloalkyl, C_3- cycloalkylC_1- alkyl, hydroxyCι- alkyl, C_1-4alkoxyC_1- alkyl, C_2-6alkenyl or C_2-6alkynyl;

R¹¹ is hydrogen, halogen, hydroxy or C_1-4alkyl; or R¹⁰ and R^π may together represent an oxo (=O) group, with the proviso that the oxo group is not adjacent to the oxygen atom represented by either A or B; wherein one or both of R¹⁰ and R¹¹ may replace one or both hydrogen atoms in the CH₂ group represented by A or B; wherein when W is

R¹⁰ is hydrogen, C_1-4alkyl, C_3-7cycloalkyl, C_3- cycloalkylC_1-4alkyl, C_2-4alkyl substituted by a C^alkoxy or hydroxyl group;

R^u is hydrogen or C_1-4alkyl, C_3- cycloalkyl, C_3- cycloalkylC_1- alkyl, or C_1-4alkyl substituted by Cι- alkoxy, hydroxyl or phenyl, wherein said phenyl group is optionally substituted by one, two or three substituents selected from C_1-6alkyl, - ₆alkoxy, halogen and trifluoromethyl; or R¹⁰, R^u and the nitrogen atom to which they are attached form a heteroaliphatic ring of 4 to 7 ring atoms, optionally substituted by one or two groups selected from hydroxy, COR^e, CO₂R^e, C_1-4alkyl optionally substituted by a C_1- alkoxy or hydroxyl group, or Cι_-4alkoxy optionally substituted by a C_1-4.alkoxy or hydroxyl group, which heteroaliphatic ring may optionally contain an oxygen or sulphur ring atom, a group S(O) or S(O)₂ or a second nitrogen atom which will be part of a NH or NR^d moiety, where R^d is C_1- alkyl optionally substituted by hydroxy or C_1-4alkoxy, and where R^e is hydrogen, C_1- alkyl or benzyl; R¹² represents halogen, hydroxy, C_1-4alkoxy or fluoroC_1-4alkoxy, or where p is 2, then two R⁹ groups may together represent an oxo (=O) group; m is zero, 1 or 2; n is 1, 2 or 3, with the proviso that the sum total of m + n is 2 or 3; and p is zero, 1 or 2; wherein when W is

A represents NR¹² or S(O)_q;

B represents NR¹² or S(O)_q, with the proviso that when A is S(O)_q, then B is NR¹², and when A is NR¹², then B is S(O)_q;

R¹⁰ and R¹¹ each independently represent hydrogen, fluorine, COR^e, CO₂R^e, C_1- alkyl optionally substituted by a C_1-4alkoxy or hydroxyl group, or C_1-4alkoxy optionally substituted by a C_1- alkoxy or hydroxyl group, where R^e is hydrogen, methyl ethyl or benzyl; or, when they are attached to the same carbon atom, R¹⁰ and R¹¹ may together represent =O, =CHCO₂R^a, -O(CH₂)_mO-, -CH₂O(CH₂)_p-, -CH₂OCH₂C(O)-, -CH₂OCH₂CH(OH)-, -CH₂OCH₂C(CH₃)₂-, -CH₂OC(CH₃)₂CH₂-, -C(CH₃)₂OCH₂CH₂-, -CH₂C(O)OCH₂-, -OC(O)CH₂CH₂-, -C(O)OCH₂CH₂-, -C(O)OC(CH₃)₂CH₂-, -C(O)OCH₂C(CH₃)₂-, -OCH₂(CH₂)_p-, -OC(CH₃)₂CH₂CH₂-, -OCH₂C(CH₃)₂CH₂-, -OCH₂CH₂C(CH₃)₂-, -OCH₂CH=CHCH₂-, -OCH₂CH(OH)CH₂CH₂-, -OCH₂CH₂CH(OH)CH₂-, -OCH₂C(O)CH₂CH₂-, -OCH₂CH₂C(O)CH₂-, or a group of the formula

where R^a is as previously defined, or, where they are attached to adjacent carbon atoms, R and R may together represent -OCH₂CH₂- or -OCH₂CH(OH)-, or R¹⁰ and R¹¹ may together form a fused benzene ring; or, R¹⁰ and R^u together form a .ialkylene bridge across the pyrrolidine, piperidine, morpholine or piperazine ring to which they are attached;

R¹² represents hydrogen, benzyl, C_1-6alkyl, C_3-7cycloalkyl, Cs^cycloalkyl ^alkyl, COC_1-6alkyl, CO₂C_1-6alkyl or C_2- alkyl substituted by a C_1-4alkoxy or hydroxyl group;

R¹³ represents hydrogen, halogen, hydroxy, Cι_-4alkyl, hydroxyC_1-4alkyl or fluoroC_1- alkyl; m is 1 or 2; p is 1, 2 or 3; and q is zero, 1 or 2; wherein when W is

R¹⁰ represents halogen, hydroxy, Cι- alkoxy or fluoroC_1- alkoxy, or where p is 2, then two R¹⁰ groups may together represent an oxo (=O) group;

R¹¹ represents hydrogen, benzyl, C_1- alkyl, C_3-7cycloalkyl, C_3-7cycloalkylCι_-4alkyl, or C_2- alkyl substituted by a C_1- alkoxy or hydroxyl group; m is zero, 1 or 2; n is zero or 1, with the proviso that the sum total of m + n is 1, 2 or 3, and with the further proviso that when m is 2 then n is 1; and p is zero, 1 or 2; wherein when W is

X represents -CHF-, -CF₂-, -CH(OH)- or -CH(OC_1-4alkyl)-;

Y represents -NR . 13T R_J 14 or a group selected from:

Z represents O, S, SO, SO₂ or NR 1¹2.

R ■ 10 represents hydrogen, halogen, hydroxy, C_1- alkoxy or fluoroCι_-4alkoxy;

R ,π represents hydrogen, halogen, hydroxy, C_1- alkoxy or fluoroCι- alkoxy; or R¹⁰ and R¹¹ together represent an oxo (=O) group;

R¹² represents C_1-6alkyl, COC_1-6alkyl, CO₂C_1-6alkyl;

R¹³ and R¹⁴ each independently represent hydrogen, C_1-6alkyl, C_3-7cycloalkyl or fluoroC_1-6alkyl, wherein said alkyl or cycloalkyl groups are optionally substituted with a group selected from hydroxy, C_1-4alkoxy, -NHCOC_1-4alkyl or -NHCO₂Cι. ₄alkyl; m is 1 or 2; n is 1 or 2; and p is 1 or 2; wherein when W is

X represents -CBR ■ 13 -, -CF₂- or -C(=O)-

R ,ιo represents halogen, hydroxy, C_1-4alkoxy or fluoroC_1- alkoxy, or where p is

2, then two R , 10 groups may together represent an oxo (=O) group; R¹¹ represents hydrogen, benzyl, C_1-6alkyl, C_3-7cycloalkyl, C_3-7cycloalkylC_1- alkyl, COC_1-6alkyl, CO Ci-₆alkyl or C_2-4alkyl substituted by a C _-4alkoxy or hydroxyl group;

R¹² represents hydrogen, fluorine, hydroxy, C_1- alkoxy, or R¹² is linked to R 11 such that there is formed a ring, the linkage -R¹²-R^u- being selected from:

(a) -OC(O)-,

(b) -OS(O)₂-,

(c) -ZCH₂CH₂-,

(d) — ZCH₂CH CH₂-,

(e) -ZCH₂C(O)-,

(f) -ZCH₂CH₂C(O)-,

(g) -ZC(O)CH₂-,

(h) -ZC(O)CH₂CH₂-, and

(i) -ZCH₂C(O)CH₂-; wherein Z represents O, NH or N(C_1-6alkyl); m is 1 or 2; and p is zero, 1 or 2.

One favoured group of compounds of the present invention are those of the formula (la) and pharmaceutically acceptable salts thereof:

wherein R¹, R², R³, R⁴, R⁵, R⁶, R⁷, R⁸ and R⁹ as hereinbefore defined, and A, B, R¹⁰, R¹¹, m and n are as hereinbefore defined as for when W is

A preferred class of compounds of formula (la) is that wherein R¹ is hydrogen, C_1-4alkyl,

halogen or CF₃.

Another preferred class of compounds of formula (la) is that wherein R is hydrogen, C^alkyl, C_1- alkoxy, halogen or CF₃.

A particularly preferred class of compounds of formula (la) is that wherein R¹ is fluorine, chlorine, methyl or CF3.

Another particularly preferred class of compounds of formula (la) is that wherein R² is hydrogen, fluorine, chlorine, methyl or CF3.

Also particularly preferred is the class of compounds of formula (la) wherein R³ is hydrogen, fluorine, chlorine, methyl or CF₃.

Preferably R¹ and R² are in the 3 and 5 positions of the phenyl ring.

More preferably R¹ is 3-fluoro, 3-chloro, 3-methyl or 3-CF₃.

More preferably R² is 5-fluoro, 5-chloro, 5-methyl or 5-CF₃.

More preferably R is hydrogen.

Most preferably R is 3-F or 3-CF₃, R is 5-CF₃ and R is hydrogen.

A further preferred class of compound of formula (la) is that wherein R⁴ is fluorine or hydrogen.

Another preferred class of compounds of formula (la) is that wherein R⁵ is hydrogen, fluorine, chlorine, bromine or CF₃.

Preferably R⁴ is hydrogen or 3-fluoro and R⁵ is hydrogen or 4-fluoro.

R⁶ is preferably C_1- alkyl optionally substituted by hydroxy. In particular, R⁶ is preferably a methyl or hydroxymethyl group.

Another preferred class of compound of formula (la) is that wherein R⁷ is hydrogen or methyl, and especially hydrogen.

A further preferred class of compound of formula (la) is that wherein one of ft Q 5£ 0

R and R is hydrogen, and especially wherein R and R are both hydrogen atoms.

Another preferred class of compound of formula (la) is that wherein R¹⁰ represents hydroxy, C_1-4alkyl or C_1- alkoxy. A further preferred class of compound of formula (la) is that wherein R ,1^l1 represents hydrogen or methyl.

A particularly preferred class of compound of formula (la) is that wherein R¹⁰ represents hydroxy and R^π represents hydrogen or methyl (especially hydrogen), where both R¹⁰ and R^π are attached to the same carbon atom, or alternatively R¹⁰ and R¹ together represent an oxo group.

A further preferred class of compound of formula (la) is that wherein A is CH₂ and B is an oxygen atom. When A is CH₂ and B is an oxygen atom it is preferred that the groups R¹⁰ and R¹¹ are not adjacent to B.

Another preferred class of compound of formula (la) is that wherein n is 2 and m is 2.

Also preferred are the classes of compound of formula (la) wherein n is 1 and m is 3; or n is 1 and m is 2; or n is 1 and m is 1.

One favoured group of compounds of the formula (la) are those of the formula (Iaa) and pharmaceutically acceptable salts thereof:

wherein

A¹ is fluorine or CF₃;

A² is fluorine or CF₃;

A³ is hydrogen or fluorine;

A⁴ is hydrogen, fluorine or bromine;

A _> 5³ : is methyl or hydroxymethyl; and A, B, R¹⁰ and R are as defined in relation to formula (la). Another favoured group of compounds of the formula (la) are those of the formula (lab) and pharmaceutically acceptable salts thereof:

wherein

X represents CHOH or C=O;

R¹ is fluorine or trifluoromethyl;

R² is fluorine or trifluoromethyl;

R³ is methyl or hydroxymethyl;

R⁴ is hydrogen or fluorine; and

R⁵ is hydrogen or fluorine; and pharmaceutically acceptable salts thereof.

A preferred class of compounds of formula (lab) is that wherein R¹ is trifluoromethyl.

Another preferred class of compounds of formula (lab) is that wherein R² is trifluoromethyl.

Also particularly preferred is the class of compounds of formula (lab) wherein R³ is methyl.

A further preferred class of compound of formula (lab) is that wherein R⁴ is fluorine.

Another preferred class of compounds of formula (lab) is that wherein R⁵ is hydrogen. Another preferred class of compound of formula (lab) is that wherein X is CHOH.

One favoured group of compounds of the formula (lab) are those of the formula (laba) and pharmaceutically acceptable salts thereof:

wherein

A¹ is hydrogen or fluorine, especially hydrogen; and A² is hydrogen or fluorine, especially fluorine.

Another favoured group of the compounds of the present invention are those of the formula (lb) and pharmaceutically acceptable salts thereof:

wherein R¹, R², R³, R⁴, R⁵ and R⁶ are hereinbefore defined;

R¹⁰, R¹¹, R¹², m, n and p are as hereinbefore defined as for when W is

R⁸ represents halogen, C_1-6alkyl, CH₂OR^c, CO₂R^a or CONR^aR^b, or where q is 2, then two R⁸ groups may together represent an oxo (=O) group, where R^a and R^b are as previously defined and R^c represents hydrogen, C_1-6alkyl or phenyl; and q is zero, 1 or 2.

A preferred class of compounds of formula (R) is that wherein R¹ is hydrogen, C_1-4alkyl, C_1-4alkoxy, halogen or CF₃.

Another preferred class of compounds of formula (lb) is that wherein R is hydrogen, C_1- alkyl, C_1-4alkoxy, halogen or CF₃.

A particularly preferred class of compounds of formula (lb) is that wherein R¹ is fluorine, chlorine, methyl or CF₃.

Another particularly preferred class of compounds of formula (lb) is that wherein R² is hydrogen, fluorine, chlorine, methyl or CF₃.

Also particularly preferred is the class of compounds of formula (lb) wherein R³ is hydrogen, fluorine, chlorine, methyl or CF₃.

Preferably R and R are in the 3 and 5 positions of the phenyl ring.

More preferably R¹ is 3-fluoro, 3-chloro, 3-methyl or 3-CF₃.

More preferably R² is 5-fluoro, 5-chloro, 5-methyl or 5-CF₃.

More preferably R³ is hydrogen.

Most preferably R¹ is 3-F or 3-CF₃, R² is 5-CF₃ and R³ is hydrogen.

A further preferred class of compound of formula (lb) is that wherein R⁴ is hydrogen or fluorine.

Another preferred class of compounds of formula (lb) is that wherein R⁵ is hydrogen, fluorine, chlorine or CF₃.

Preferably R⁴ is hydrogen or 3-fluoro and R⁵ is hydrogen or 4-fluoro. R⁶ is preferably C_1-4alkyl optionally substituted by hydroxy. In particular, R⁶ is preferably a methyl or hydroxymethyl group. Most especially, R⁶ is a methyl group.

A further preferred class of compounds of formula (lb) is that wherein R¹⁰ is hydrogen, C_1- alkyl or a C_2-4alkyl group substituted by a hydroxyl or C_1-2alkoxy group.

Another preferred class of compounds of formula (lb) is that wherein R is hydrogen, C_1- alkyl, or a benzyl group.

Where R¹⁰ and R¹¹ are linked so that, together with the nitrogen atom to which they are attached, they form a heteroaliphatic ring, suitable rings include azetidinyl, pyrrolidinyl, piperidinyl, morpholino, thiomorpholino, piperazino or a piperazino group substituted on the nitrogen atom by a C_1-4alkyl group or a C_2-4alkyl group substituted by a hydroxy or C_1-2alkoxy group. Particularly preferred heteroaliphatic rings formed by -NR¹⁰R^π are azetidine, pyrrolidine, piperidine, morpholine, piperazine and N-methylpiperazine.

Another preferred class of compounds of formula (lb) is that wherein R is fluorine, hydroxy, methoxy or ethoxy or, where p is 2, then the two R¹² groups together represent an oxo (=O) group.

Another preferred class of compounds of formula (lb) is that wherein R is methyl.

Another preferred class of compounds of formula (lb) is that wherein m is zero or 1 and n is 1 or 2. Most preferably, m is zero and n is 2.

Yet another preferred class of compounds of formula (lb) is that wherein p is zero.

A further preferred class of compounds of formula (lb) is that wherein q is zero.

It will be appreciated that where one or two R¹² groups are present, each group may replace any of the hydrogen atoms in the (CH₂)_m and (CF£₂)_n groups. Similarly, the -CH -NR¹⁰Rⁿ moiety may replace a hydrogen atom in either of the (CH )_m and (CH₂)_n groups.

One favoured group of compounds of the formula (lb) are those of the formula (Iba) and pharmaceutically acceptable salts thereof:

wherein

A¹ is fluorine or CF₃;

A² is fluorine or CF₃;

A³ is fluorine or hydrogen;

A⁴ is fluorine or hydrogen;

A⁵ is methyl; and

R¹⁰, R^π, R¹², m, n and p are as defined in relation to formula (lb).

Another favoured group of the compounds of the present invention are those of the formula (Ic) and pharmaceutically acceptable salts thereof:

wherein R , R , R , R , R and R are as hereinbefore defined;

A, B, R¹⁰, R¹¹, R¹², R¹³, m, p and q are as hereinbefore defined as for when W is

R⁸ represents halogen, C_1-6alkyl, CH₂OR^c, CO₂R^a or CONR^aR^b, or where n is 2, then two R⁸ groups may together represent an oxo (=O) group, where R^a and R^b are as previously defined and R^c represents hydrogen, C_1-6alkyl or phenyl; and n is zero, 1 or 2.

A preferred class of compounds of formula (Ic) is that wherein R¹ is hydrogen, C_1-4alkyl, C_1-4alkoxy, halogen or CF₃.

Another preferred class of compounds of formula (Ic) is that wherein R is hydrogen, C_1-4alkyl, C_1- alkoxy, halogen or CF₃.

A particularly preferred class of compounds of formula (Ic) is that wherein R¹ is fluorine, chlorine, methyl or CF₃.

Another particularly preferred class of compounds of formula (Ic) is that wherein R is hydrogen, fluorine, chlorine, methyl or CF₃.

Also particularly preferred is the class of compounds of formula (Ic) wherein R is hydrogen, fluorine, chlorine, methyl or CF₃.

1 9

Preferably R and R are in the 3 and 5 positions of the phenyl ring.

More preferably R¹ is 3-fluoro, 3-chloro, 3-methyl or 3-CF .

More preferably R² is 5-fluoro, 5-chloro, 5-methyl or 5-CF₃.

More preferably R³ is hydrogen.

Most preferably R¹ is 3-F or 3-CF₃, R² is 5-CF₃ and R³ is hydrogen.

A further preferred class of compound of formula (Ic) is that wherein R⁴ is hydrogen or fluorine.

Another preferred class of compounds of formula (Ic) is that wherein R⁵ is hydrogen, fluorine, chlorine or CF₃.

Preferably R⁴ is hydrogen or 3-fluoro and R⁵ is hydrogen or 4-fluoro.

R is preferably C_1-4alkyl optionally substituted by hydroxy. In particular, R is preferably a methyl or hydroxymethyl group. Most especially, R⁶ is a methyl group. Another preferred class of compounds of formula (Ic) is that wherein R , 10 i ■s hydrogen, hydroxy, C_1-2alkyl substituted by hydroxy, C_1- alkoxy (especially methoxy) or CO₂R^e (where R^e is hydrogen, methyl ethyl or benzyl). Particularly preferred are compounds of formula (Ic) where R¹⁰ is hydrogen.

A further pre erred class of compounds of formula (Ic) is that wherein R ¹ is hydrogen or C_1- alkyl (especially methyl). Particularly preferred are compounds of formula (I) where R¹¹ is hydrogen.

R¹⁰ and R¹¹ are preferably attached to the same carbon atom thus forming a moiety of the formula CR¹⁰R^π. Where R¹⁰ and R¹¹ are attached to the same carbon atom they may, in particular, together represent -C(O)OCH₂CH₂-.

In a further preferred class of compounds of formula (Ic), R preferably represents hydrogen, methyl or ethyl.

Another preferred class of compounds of formula (Ic) is that wherein R is methyl.

A further preferred class of compounds of formula (Ic) is that wherein n is zero.

A further preferred class of compounds of formula (Ic) is that wherein A is NR¹² andB is S(O)_q.

Preferably, q is zero.

One favoured group of compounds of the formula (Ic) are those of the formula (lea) and pharmaceutically acceptable salts thereof:

wherein

A¹ is fluorine or CF₃;

A² is fluorine or CF₃;

A is fluorine or hydrogen;

A⁴ is fluorine or hydrogen;

A⁵ is methyl; and

A, B, R¹⁰ and R¹¹ are as defined in relation to formula (Ic).

Another favoured group of the compounds of the present invention are those of the formula (Id) and pharmaceutically acceptable salts thereof:

wherein R¹, R², R³, R⁴, R⁵ and R⁶ are as hereinbefore defined;

R .10 , τ R>ll , m, n and p are as hereinbefore defined as for when W is

R⁸ represents halogen, C_1- alkyl, CH₂OR^c, CO₂R^a or CONR^aR^b, or where q is 2, then two R⁸ groups may together represent an oxo (=O) group, where R^a and R are as previously defined and R^c represents hydrogen, C_1- alkyl or phenyl; and q is zero, 1 or 2. A preferred class of compounds of formula (Id) is that wherein R¹ is hydrogen, C_1-4alkyl, C_1-4alkoxy, halogen or CF₃.

Another preferred class of compounds of formula (Id) is that wherein R² is hydrogen, C_1- alkyl, Cι_-4alkoxy, halogen or CF₃.

A particularly preferred class of compounds of formula (Id) is that wherein R¹ is fluorine, chlorine, methyl or CF₃.

Another particularly preferred class of compounds of formula (Id) is that wherein R² is hydrogen, fluorine, chlorine, methyl or CF₃.

Also particularly preferred is the class of compounds of formula (Id) wherein R³ is hydrogen, fluorine, chlorine, methyl or CF₃.

Preferably R and R are in the 3 and 5 positions of the phenyl ring.

More preferably R¹ is 3-fluoro, 3-chloro, 3-methyl or 3-CF₃.

More preferably R² is 5-fluoro, 5-chloro, 5-methyl or 5-CF₃.

More preferably R³ is hydrogen.

Most preferably R¹ is 3-F or 3-CF₃, R² is 5-CF₃ and R³ is hydrogen

A A ffuurtrthheerr pprreef] erred class of compound of formula (Id) is that wherein R⁴ is hydrogen or fluorine.

Another preferred class of compounds of formula (Id) is that wherein R⁵ is hydrogen, fluorine, chlorine or CF₃.

Preferably R⁴ is hydrogen or 3-fluoro and R⁵ is hydrogen or 4-fluoro.

R is preferably C_1- alkyl optionally substituted by hydroxy. In particular, R is preferably a methyl or hydroxymethyl group. Most especially, R⁶ is a methyl group.

Another preferred class of compounds of formula (Id) is that wherein R¹⁰ is fluorine, hydroxy, methoxy or ethoxy or, where p is 2, then the two R¹⁰ groups together represent an oxo (=O) group.

In a further preferred class of compounds of formula (Id), R¹¹ preferably represents hydrogen, methyl or ethyl.

Another preferred class of compounds of formula (Id) is that wherein R is methyl.

Another preferred class of compounds of formula (Id) is that wherein m is zero or 1 and n is zero or 1, and the sum total of m + n is 1. Most preferably, m is zero and n is l. A further preferred class of compound of formula (Id) is that wherein m is 2 and n is 1.

Yet another preferred class of compounds of formula (Id) is that wherein p is zero.

A further preferred class of compounds of formula (Id) is that wherein q is zero.

It will be appreciated that where one or two R¹⁰ groups are present, each group may replace any of the hydrogen atoms in the (CH₂)_m and (CH₂)_n groups.

One favoured group of compounds of the formula (Id) are those of the formula (Ida) and pharmaceutically acceptable salts thereof:

wherein

A 1 i •s fluorine or CF₃; A² is fluorine or CF₃; A³ is fluorine or hydrogen; A⁴ is fluorine or hydrogen; A⁵ is methyl; and

R¹ , R¹¹, m, n and p are as defined in relation to formula (Id). Another favoured group of the compounds of the present invention are those of formula (le) and pharmaceutically acceptable salts thereof:

wherein R¹, R², R³, R⁴, R⁵ and R⁶ are as hereinbefore defined;

X, Y, Z, R¹⁰, R¹¹, R¹², R¹³, R¹⁴, m, n and p are as hereinbefore defined as for when W is

R⁸ represents halogen, -ealkyl, CH₂OR^c, CO₂R^a or CONR^aR^b, or where q is 2, then two R⁸ groups may together represent an oxo (=O) group, where R^a and R^b are as previously defined and R^c represents hydrogen, C_halky! or phenyl; and q is zero, 1 and 2.

A preferred class of compounds of formula (le) is that wherein X is -CH(OH)-.

Another preferred class of compounds of formula (le) is that wherein Y is -NR¹³R¹⁴ (wherein R¹³ and R¹⁴ are preferably hydrogen or C_1- alkyl) or the group

wherein R¹⁰ and R¹¹ are preferably hydrogen and m is 1 or 2, and especially 2.

A further preferred class of compounds of formula (le) is that wherein Z is O, S or NR¹², especially where R¹² is a C_1- alkyl group, such as methyl or ethyl. A preferred class of compounds of formula (le) is that wherein R¹ is hydrogen, C_1-4alkyl, C_1-4alkoxy, halogen or CF₃.

Another preferred class of compounds of formula (le) is that wherein R² is hydrogen, C_halky!, C_1- alkoxy, halogen or CF₃.

A particularly preferred class of compounds of formula (le) is that wherein R¹ is fluorine, chlorine, methyl or CF₃.

Another particularly preferred class of compounds of formula (le) is that wherein R² is hydrogen, fluorine, chlorine, methyl or CF₃.

Also particularly preferred is the class of compounds of formula (le) wherein R³ is hydrogen, fluorine, chlorine, methyl or CF₃.

Preferably R and R are in the 3 and 5 positions of the phenyl ring.

More preferably R¹ is 3-fluoro, 3-chloro, 3-methyl or 3-CF₃.

More preferably R² is 5-fluoro, 5-chloro, 5-methyl or 5-CF₃.

More preferably R is hydrogen.

Most preferably R¹ is 3-F or 3-CF₃, R² is 5-CF₃ and R³ is hydrogen.

A further preferred class of compound of formula (le) is that wherein R⁴ is hydrogen or fluorine.

Another preferred class of compounds of formula (le) is that wherein R⁵ is hydrogen, fluorine, chlorine or CF₃.

P 'rreeferably R⁴ is hydrogen or 3-fluoro and R⁵ is hydrogen or 4-fluoro.

R⁶ is preferably C_1- alkyl optionally substituted by hydroxy. In particular, R⁶ is preferably a methyl or hydroxymethyl group. Most especially, R⁶ is a methyl group.

Another preferred class of compounds of formula (le) is that wherein R¹⁰ is hydrogen, fluorine, hydroxy, methoxy or ethoxy and R¹¹ is hydrogen, or R¹⁰ and R^π are both fluorine or R¹⁰ and R¹¹ together represent an oxo (=O) group.

Another preferred class of compounds of formula (le) is that wherein R⁸ is methyl.

A further preferred class of compounds of formula (le) is that wherein R is methyl, ethyl, acetyl, methoxycarbonyl or ethoxycarbonyl.

Another preferred class of compounds of formula (le) is that wherein R and R¹⁵ each independently represent hydrogen or C_1-4alkyl, and especially, methyl.

Another preferred class of compounds of formula (le) is that wherein m is 2. A further preferred class is that wherein m is 2 and n is 2.

Yet another preferred class of compounds of formula (le) is that wherein p is

1.

A further preferred class of compounds of formula (le) is that wherein q is zero.

It will be appreciated that R¹⁰ and R¹¹ may replace any of the hydrogen atoms in the (CH )_m and (CH₂)_P groups in the rings to which they are attached.

One favoured group of compounds of the formula (le) are those of the formula (lea) and pharmaceutically acceptable salts thereof:

wherein

A¹ is fluorine or CF₃;

A² is fluorine or CF₃;

A³ is fluorine or hydrogen;

A⁴ is fluorine or hydrogen;

A⁵ is methyl; and

X and Y are as defined in relation to formula (le).

Another favoured group of compounds of the present invention are those of formula (If) and pharmaceutically acceptable salts thereof:

wherein R¹, R², R³, R⁴, R⁵ and R⁶ are as hereinbefore defined;

X, R¹⁰, R¹¹, R¹², m and p are as hereinbefore defined as for when W is

R° represents halogen, C_1-6alkyl, CH₂OR°, CO₂R^d or CONR -"_TR₎b^D, or where q is 2, then two R⁸ groups may together represent an oxo (=O) group, where R^a and R^b are as previously defined and R^c represents hydrogen, C_1-6alkyl or phenyl; and q is zero, 1 or 2.

A preferred class of compounds of formula (If) is that wherein R¹ is hydrogen, C_1-4alkyl, C_1-4alkoxy, halogen or CF₃.

Another preferred class of compounds of formula (If) is that wherein R² is hydrogen, C_1-4alkyl, C_1- alkoxy, halogen or CF₃.

A particularly preferred class of compounds of formula (If) is that wherein R¹ is fluorine, chlorine, methyl or CF₃.

Another particularly preferred class of compounds of formula (If) is that wherein R is hydrogen, fluorine, chlorine, methyl or CF3.

Also particularly preferred is the class of compounds of formula (If) wherein R³ is hydrogen, fluorine, chlorine, methyl or CF₃.

Preferably R¹ and R² are in the 3 and 5 positions of the phenyl ring. More preferably R¹ is 3-fluoro, 3-chloro, 3-methyl or 3-CF₃.

More preferably R² is 5-fluoro, 5-chloro, 5-methyl or 5-CF₃.

More preferably R³ is hydrogen.

Most preferably R¹ is 3-F or 3-CF₃, R² is 5-CF₃ and R³ is hydrogen.

A further preferred class of compound of formula (If) is that wherein R⁴ is hydrogen or fluorine.

Another preferred class of compounds of formula (If) is that wherein R⁵ is hydrogen, fluorine, chlorine or CF₃.

Preferably R⁴ is hydrogen or 3-fluoro and R⁵ is hydrogen or 4-fluoro.

R is preferably C_1- alkyl optionally substituted by hydroxy. In particular, R is preferably a methyl or hydroxymethyl group. Most especially, R is a methyl group.

Another preferred class of compounds of formula (If) is that wherein R¹⁰ is fluorine, hydroxy, methoxy or ethoxy or, where p is 2, then the two R¹⁰ groups together represent an oxo (=O) group.

In a further preferred class of compounds of formula (If), R¹¹ preferably represents hydrogen, methyl, ethyl or CO₂-tert-butyl.

Another preferred class of compounds of formula (If) is that wherein R⁸ is methyl.

A further preferred class of compound of formula (If) is that wherein X is -CHR¹²-.

In particular, R¹² is preferably hydrogen or hydroxy.

Where R¹² is linked to R¹¹ such that there is formed a ring, the linkage -R¹²- R¹¹- is preferably selected from:

(a) -OC(O)-,

(b) -OS(O)₂-,

(c) -ZCH₂CH₂-,

(d) -ZCH₂C(O)-,

(e) -ZC(O)CH₂-, wherein Z represents O, NH or N(C_1-6alkyl). Most especially, where R¹² is linked to R^π such that there is formed a ring, the linkage -R¹²-R^π- is preferably -OC(O)-.

Another preferred class of compounds of formula (If) is that wherein m is 1. Yet another preferred class of compounds of formula (If) is that wherein p is zero.

A further preferred class of compounds of formula (If) is that wherein q is zero.

It will be appreciated that where one or two R ,10 groups are present, each group may replace any of the hydrogen atoms in the (CH )_m groups.

One favoured group of compounds of the present invention are those of the formula (Ifa) and pharmaceutically acceptable salts thereof:

wherein

A¹ is fluorine or CF₃;

A² is fluorine or CF₃;

A³ is fluorine or hydrogen;

A⁴ is fluorine or hydrogen;

A⁵ is methyl; and

X, R¹⁰, R¹¹, m and p are as defined in relation to formula (If).

When any variable occurs more than one time in formula (I) or in any substituent, its definition on each occurrence is independent of its definition at every other occurrence.

As used herein, the term "alkyl" or "alkoxy" as a group or part of a group means that the group is straight or branched. Examples of suitable alkyl groups include methyl, ethyl, n-propyl, i-propyl, n-butyl, s-butyl and t-butyl. Examples of suitable alkoxy groups include methoxy, ethoxy, n-propoxy, i-propoxy, n-butoxy, s- butoxy and t-butoxy.

As used herein, the terms "fluoro -_δal yr and "fluoroCi-βallcoxy" mean a Ci-βalkyl or C_1-6alkoxy group in which one or more (in particular, 1 to 3) hydrogen atoms have been replaced by fluorine atoms. Similarly, the term

means a C_1- alkyl group in which one or more (in particular 1 to 3) hydrogen atoms have been replaced by fluorine atoms. Particularly preferred are fluoro .salkyl and fluoroC_1-3alkoxy groups, for example, CF₃, CH₂CH₂F, CH₂CHF , CH₂CF₃, OCF₃, OCH₂CH₂F, OCH₂CHF₂ or OCH₂CF₃, and most especially CF₃, OCF₃ and OCH₂CF₃.

The cycloalkyl groups referred to herein may represent, for example, cyclopropyl, cyclobutyl, cyclopentyl or cyclohexyl. A suitable cycloalkylalkyl group may be, for example, cyclopropylmethyl.

Similarly cycloalkoxy groups referred to herein may represent, for example, cyclopropoxy or cyclobutoxy.

As used herein, the terms "alkenyl" and "alkynyi" as a group or part of a group mean that the group is straight or branched. Examples of suitable alkenyl groups include vinyl and allyl. A suitable alkynyi group is propargyl.

When used herein the term "halogen" means fluorine, chlorine, bromine and iodine. The most apt halogens are fluorine and chlorine of which fluorine is preferred, unless otherwise stated.

Specific compounds within the scope of this invention include: (4R5)-8-[(2R,3R,4i?)-2-[(lR)-l-(3,5-bis(trifluoromethyl)phenyl)ethoxy]-3-ρhenyl- tetrahydropyran-4-yl]methyl-2-oxa-8-aza-spiro[4.5]decan-4-ol; (45)-8-[(2R,3R,4R)-2-[(lR)-l-(3,5-bis(trifluoromethyl)phenyl)ethoxy]-3-(4- fluorophenyl)tetrahydropyran-4-yl]methyl-2-oxa-8-aza-spiro[4.5]decan-4-ol; (4R)-8-[(2R,3R,4R)-2-[(l_JR)-l-(3,5-bis(trifluoromethyl)phenyl)ethoxy]-3-(4- fluorophenyl)tetrahydropyran-4-yl]methyl-2-oxa-8-aza-spiro[4.5]decan-4-ol; (4R5)-8-[(2R,3i?,4R)-2-[(li?)-l-(3,5-bis(trifluoromethyl)phenyl)ethoxy]-3-(3,4- difluorophenyl)tetrahydropyran-4-yl]methyl-2-oxa-8-aza-sρiro[4.5]decan-4-ol; 8-[(2i?,3i?,4i?)-2-[(li?)-l-(3,5-bis(trifluoromethyl)ρhenyl)ethoxy]-3-(4- fluorophenyl)tetrahydropyran-4-yl]methyl-2-oxa-8-aza-spiro[4.5]decane; 8-[(2R,35,4S)-2-[(lR)-l-(3,5-bis(trifluoromethyl)phenyl)ethoxy]-3- phenyl(tetrahydropyran-4-yl)]methyl-l,l-dimethyl-2-oxa-8-aza-spiro[4.5]decane; 8-[(2R,35,45)-2-[(ll?)-l-(3,5-bis(trifluoromethyl)ρhenyl)ethoxy]-3- phenyl(tetrahydropyran-4-yl)]methyl-2,2-dimethyl-l-oxa-8-aza-spiro[4.5]decane;

8-[(2i?,3S,4S)-2-[(li?)-l-(3,5-bis(trifluoromethyl)phenyl)ethoxy]-3-phenyl- tetrahydroρyran-4-yl]methyl-l-oxa-8-aza-sρiro[4.5]decane;

8-[(2^,3S,4S)-2-[(l_JR)-l-(3,5-bis(trifluoromethyl)phenyl)ethoxy]-3-phenyl- tetrahydropyran-4-yl]methyl-2-oxa-8-aza-spiro[4.5]decane;

8-[(21?,3i?,4i?)-2-[(li?)-l-(3,5-bis(trifluoromethyl)ρhenyl)ethoxy]-3-phenyl- tetrahydropyran-4-yl]methyl-2-oxa-8-aza-spiro[4.5]decane;

8-[(2i?,3i?,4i?)-2-[(li?)-l-(3,5-bis(trifluoromethyl)phenyl)ethoxy]-3-phenyl- tetrahydropyran-4-yl]methyl-2,2-dimethyl-l-oxa-8-aza-spiro[4.5]decane;

8-[(2i?,3R,4i?)-2-[(li?)-l-(3,5-bis(trifluoromethyl)phenyl)ethoxy]-3-ρhenyl- tetrahydropyran-4-yl]methyl-l-oxa-8-aza-spiro[4.5]decane;

8-[(22?,3R,4R)-2-[(lR)-l-(3,5-bis(trifluoromethyl)phenyl)ethoxy]-3-phenyl- tetrahydropyran-4-yl]methyl-l,l-dimethyl-2-oxa-8-aza-spiro[4.5]decane;

8-[(2 ?,3R,4R)-2-[(lR)-l-(3,5-bis(trifluoromethyl)phenyl)ethoxy]-3-(4- fluorophenyl)tetrahydropyran-4-yl]methyl-2-oxa-8-aza-spiro[4.5]decan-4-one;

(4i?5)-8-[(2R,3R,4R)-2-[(lR)-l-(3,5-bis(trifluoromethyl)phenyl)ethoxy]-3-(4- fluorophenyl)tetrahydropyran-4-yl]methyl-2-oxa-4-methyl-8-aza-spiro[4.5]decan-4- ol; and pharmaceutically acceptable salts thereof.

Specific compounds within the scope of this invention also include: (4R)-8-[(2R,3R,4R)-2-[(lR)-l-(3,5-bis(trifluoromethyl)ρhenyl)ethoxy]-3-(3-bromo-4- fluorophenyl)tetrahydropyran-4-yl]methyl-2-oxa-8-aza-spiro[4.5]decan-4-ol; (4R5)-8-[(2R,3R,4R)-2-[(lR)-l-(3,5-bis(trifluoromethyl)phenyl)ethoxy]-(4- fluorophenyl)tetrahydropyran-4-yl]methyl-2-oxa-4-ethynyl-8-aza-spiro[4.5]decan-4- ol;

(85)-2-[((2i?,3R,4i?)-2-[(li?)-l-[3,5-bis(trifluoromethyl)phenyl]ethoxy]-3- phenyl)tetrahydro-2H-pyran-4-yl)methyl]-6-oxa-2-azaspiro[3.4]octan-8-ol;

(8I?)-2-[((2i?,3i?,4i?)-2-[(li?)-l-[3,5-bis(trifluoromethyl)ρhenyl]ethoxy]-3- phenyltetrahydro-2H-pyran-4-yl)methyl]-6-oxa-2-azaspiro[3.4]octan-8-ol; (4i?5)-8-[(2i?,3i?,4R)-2-[(li?)-l-(3,5-bis(trifluoromethyl)phenyl)ethoxy]-3- phenyltetrahydropyran-4-yl]methyl-l-oxa-8-azaspiro[4.5]decan-4-ol;

(4i?5)-8-[(2i?,3i?,41?)-2-[(li?)-l-(3,5-bis(trifluoromethyl)phenyl)ethoxy]-3-(4- fluorophenyl)tetrahydropyran-4-yl]methyl-3,3-dimethyl-2-oxa-8-azaspiro[4.5]decan- 4-ol;

(4i?*)-8-[(2i?,3i?,4_JR)-2-[(li?)-l-(3,5-bis(trifluoromethyl)phenyl)ethoxy]-3-(4- fluorophenyl)tetrahydropyran-4-yl]methyl-l-oxa-8-azaspiro[4.5]decan-4-ol;

(45*)-8-[(2i?,3^,4i?)-2-[(l^)-l-(3,5-bis(trifluoromethyl)phenyl)ethoxy]-3-(4- fluorophenyl)tetrahydropyran-4-yl]methyl-l-oxa-8-azaspiro[4.5]decan-4-ol;

(4i?)-8-[((2i?,3i?,4i?)-2-{(lR)-l-[3,5-bis(trifluoromethyl)phenyl]ethoxy}-3- phenyltetrahydro-2H-pyran-4-yl)methyl]-4-methoxy-2-oxa-8-azaspiro[4.5]decane;

(4R)-8-[((2R,3R,4R)-2-{(lR)-l-[3,5-bis(trifluoromethyl)phenyl]ethoxy}-3-(4- fluorophenyl)tetrahydro-2H-pyran-4-yl)methyl]-4-methoxy-2-oxa-8- azaspiro[4.5]decane;

8-[((2/?,3i?,4i?)-2-{(lR)-l-[3,5-bis(trifluoromethyl)phenyl]ethoxy}-3- phenyltetrahydro-2H-pyran-4-yl)methyl]-l-oxa-8-azaspiro[4.5]decan-3-ol; 8-[((2R,3R,42?)-2-{(lR)-l-[3,5-bis(trifluoromethyl)phenyl]ethoxy}-3- phenyltetrahydro-2H-pyran-4-yl)methyl]-l-oxa-8-azaspiro[4.5]decan-3-one; and pharmaceutically acceptable salts thereof.

Further specific compounds within the scope of this invention include: {2R or S,5R or 5)-5-((2R,3R,4R)-{(lR)-l-[3,5-bis(trifluoromethyl)phenyl]ethoxy}- tetrahydro-3-phenyl-2H-pyran-4-yl)tetrahydro-N-(phenylmethyl)-2- furanmethanamine;

(25 or R,5R or 5)-5-((2R,3R,4R)-{(lR)-l-[3,5-bis(trifluoromethyl)phenyl]ethoxy}- tetrahydro-3-phenyl-2H-pyran-4-yl)tetrahydro-N-(phenylmethyl)-2- furanmethanamine;

(2R or S,5R or 5)-5-((2R,3R,4R)-{(lR)-l-[3,5-bis(trifluoromethyl)ρhenyl]ethoxy}- tetrahydro-3-phenyl-2H-pyran-4-yl)tetrahydro-2-furanmethanamine; {2S or R,5R or 5)-5-((2^,3^,4i?)-{(li?)-l-[3,5-bis(trifluoromethyl)phenyl]ethoxy}- tetrahydro-3-ρhenyl-2H-pyran-4-yl)-N,N-diethyltetrahydro-2-furanmethanamine; and pharmaceutically acceptable salts thereof.

Specific compounds within the scope of this invention also include: (3R or S)-3-{{2R,3R,4R)-{ (li?)-l-[3,5-bis(trifluoromethyl)phenyl]ethoxy}tetrahydro-

3-phenyl-2H-pyran-4-yl)thiomorpholine; and

{3S or ^)-3-((2i?,3i?,4i?)-{ (li?)-l-[3,5-bis(trifluoromethyl)phenyl]ethoxy}tetrahydro-

3-phenyl-2H-pyran-4-yl)thiomorpholine; and pharmaceutically acceptable salts thereof.

Further specific compounds within the scope of this invention include: (2S)-2-((2i?,3i?,4i?)-{(li?)-l-[3,5-bis(trifluoromethyl)phenyl]ethoxy}tetrahydro-3- phenyl-2H-ρyran-4-yl)-l-methylazetidine;

(2i?)-2-((2i?,3i?,4i?)-{(l_JR)-l-[3,5-bis(trifluoromethyl)phenyl]ethoxy}tetrahydro-3- ρhenyl-2H-pyran-4-yl)- 1 -methylazetidine;

4-((2 ,3i?,4i?)-{(lR)-l-[3,5-bis(trifluoromethyl)phenyl]ethoxy}tetrahydro-3-phenyl- 2H-pyran-4-yl)piperidin-4-ol;

4-((2i?,3R,4R)-{(lR)-l-[3,5-bis(trifluoromethyl)phenyl]ethoxy}tetrahydro-3-phenyl- 2H-pyran-4-yl)-l-cyclopropylpiperidin-4-ol; and

4-((2i?,3R,4_JR)-{(lR)-l-[3,5-bis(trifluoromethyl)phenyl]ethoxy}tetrahydro-3-phenyl- 2H-pyran-4-yl)-l-(l-methylethyl)piperidin-4-ol; and pharmaceutically acceptable salts thereof.

Specific compounds within the scope of this invention also include: (αi? or S)-α-((2R,3R,4R)-{(lR)-l-[3,5-bis(trifluoromethyl)phenyl]ethoxy}tetrahydro- 3-phenyl-2H-pyran-4-yl)-l-piperidineethanol;

(α5 orR)-α-((2R,3i?,4R)-{(lR)-l-[3,5-bis(trifluoromethyl)phenyl]ethoxy}tetrahydro- 3-phenyl-2H-pyran-4-yl)-l-piperidineethanol; and

(αR or 5',2i?,3R,4R)-2-{ (lR)-l-[3,5-bis(trifluoromethyl)ρhenyl]ethoxy }tetrahydro-α- [(dimethylamino)methyl]-3-phenyl-2H-pyran-4-methanol; and pharmaceutically acceptable salts thereof.

Further specific compounds within the scope of this invention include: 1,1-dimethylethyl {2R or S)-2-[{R or 5)-((2R,3i?,4R)-{(lR)-l-[3,5- bis(trifluoromethyl)phenyl]ethoxy}tetrahydro-3-phenyl-2H-pyran-4- yl)hydroxymethyl]-l-pyrrolidinecarboxylate;

(IR or S, 7aR or 5)-l-((2R,3R,4R)-{(lR)-l-[3,5-bis(trifluoromethyl)phenyl]- ethoxy } tetrahydro-3-phenyl-2H-pyran-4-yl)tetrahydro- lH,3H-pyrrolo [ 1 ,2-c] oxazol-3 - one; and (2R,3R,4R,αR or S)-α-[(2R or S)-2~pyrrolidinyl]-2-{(lR)-l-[3,5- bis(trifluoromethyl)phenyl]ethoxy}tetrahydro-3-phenyl-2H-pyran-4-methanol; and pharmaceutically acceptable salts thereof.

In a further aspect of the present invention, the compounds of formula (I) may be prepared in the form of a pharmaceutically acceptable salt, especially an acid addition salt.

For use in medicine, the salts of the compounds of formula (I) will be non- toxic pharmaceutically acceptable salts. Other salts may, however, be useful in the preparation of the compounds according to the invention or of their non-toxic pharmaceutically acceptable salts. Suitable pharmaceutically acceptable salts of the compounds of this invention include acid addition salts which may, for example, be formed by mixing a solution of the compound according to the invention with a solution of a pharmaceutically acceptable acid such as hydrochloric acid, fumaric acid, p-toluenesulfonic acid, maleic acid, succinic acid, acetic acid, citric acid, tartaric acid, carbonic acid, phosphoric acid or sulfuric acid. Salts of amine groups may also comprise quaternary ammonium salts in which the amino nitrogen atom carries a suitable organic group such as an alkyl, alkenyl, alkynyi or aralkyl moiety. Furthermore, where the compounds of the invention carry an acidic moiety, suitable pharmaceutically acceptable salts thereof may include metal salts such as alkali metal salts, e.g. sodium or potassium salts; and alkaline earth metal salts, e.g. calcium or magnesium salts.

The salts may be formed by conventional means, such as by reacting the free base form of the product with one or more equivalents of the appropriate acid in a solvent or medium in which the salt is insoluble, or in a solvent such as water which is removed in vacuo or by freeze drying or by exchanging the anions of an existing salt for another anion on a suitable ion exchange resin.

The present invention includes within its scope prodrugs of the compounds of formula (I) above. In general, such prodrugs will be functional derivatives of the compounds of formula (I) which are readily convertible in vivo into the required compound of formula (I). Conventional procedures for the selection and preparation of suitable prodrug derivatives are described, for example, in "Design of Prodrugs", ed. Η. Bundgaard, Elsevier, 1985. A prodrug may be a pharmacologically inactive derivative of a biologically active substance (the "parent drug" or "parent molecule") that requires transformation within the body in order to release the active drug, and that has improved delivery properties over the parent drug molecule. The transformation in vivo may be, for example, as the result of some metabolic process, such as chemical or enzymatic hydrolysis of a carboxylic, phosphoric or sulfate ester, or reduction or oxidation of a susceptible functionality.

The present invention includes within its scope solvates of the compounds of formula (I) and salts thereof, for example, hydrates.

The compounds according to the invention have at least three asymmetric centres, and may accordingly exist both as enantiomers and as diastereoisomers. It is to be understood that all such isomers and mixtures thereof are encompassed within the scope of the present invention.

The preferred compounds of the formulae (la) and (Iaa) will have the stereochemistry of the 2-, 3-, 4- and 8-positions as shown in formulae (lac) and (lad)

It will be appreciated that the preferred definitions of the various substituents recited herein may be taken alone or in combination and, unless otherwise stated, apply to the generic formula for compounds of the present invention as well as to the preferred classes of compound represented by formula (Iaa), formula (lac) and formula (lad).

The preferred compounds of the formulae (lab) and (laba) will have the stereochemistry of the 2-, 3-, 4- and 8-positions as shown in formulae (Iabb) and (Iabc)

It will be appreciated that the preferred definitions of the various substituents recited herein may be taken alone or in combination and, unless otherwise stated, apply to the generic formula for compounds of the present invention as well as to the preferred classes of compound represented by formula (laba), formula (labb) and formula (labc).

The preferred compounds of the formulae (lb), (Iba), (Id), (Ida), (le), (lea), (If) and (Ifa) will have the stereochemistry of the 2-, 3-, 4- and 8-positions as shown in formula (Ig) and formula (Ih)

It will be appreciated that the preferred definitions of the various substituents recited herein may be taken alone or in combination and, unless otherwise stated, apply to the generic formula for compounds of formulae (lb), (Id), (le) and (If) as well as to the preferred classes of compound represented by formulae (Iba), (Ida), (lea), (Ifa), (Ig) and (Ih). The preferred compounds of the formula (Ic) and (lea) will have the stereochemistry of the 2-, 3-, 4- and 8-positions as shown in formula (Icb) and formula (Ice)

It will be appreciated that the preferred definitions of the various substituents recited herein may be taken alone or in combination and, unless otherwise stated, apply to the generic formula for compounds of formula (Ic) as well as to the preferred classes of compound represented by formula (lea), formula (Icb) and formula (Ice).

The present invention further provides pharmaceutical compositions comprising one or more compounds of formula (I) in association with a pharmaceutically acceptable carrier or excipient.

Preferably the compositions according to the invention are in unit dosage forms such as tablets, pills, capsules, powders, granules, solutions or suspensions, or suppositories, for oral, parenteral or rectal administration, or administration by inhalation or insufflation. Oral compositions such as tablets, pills, capsules or wafers are particularly preferred.

A more detailed description of pharmaceutical compositions that are suitable for the formulation of compounds of the present invention is disclosed in US patent No. 6,071,927, the content of which is incorporated herein by reference (see in particular, column 8, line 50 to column 10, line 4).

The present invention further provides a process for the preparation of a pharmaceutical composition comprising a compound of formula (I), which process comprises bringing a compound of formula (I) into association with a pharmaceutically acceptable carrier or excipient.

The compounds of formula (I) are of value in the treatment of a wide variety of clinical conditions which are characterised by the presence of an excess of tachykinin, in particular substance P, activity. A comprehensive listing of clinical conditions, uses and methods of treatment for which the compounds of the present invention will be useful is disclosed in US patent No. 6,071,927, the content of which is incorporated herein by reference (see, in particular, column 10, line 14 to column 22, line 18).

In particular, the compounds of the present invention are useful in the treatment of a variety of disorders of the central nervous system. Such disorders include mood disorders, such as depression or more particularly depressive disorders, for example, single episodic or recurrent major depressive disorders and dysthymic disorders, or bipolar disorders, for example, bipolar I disorder, bipolar II disorder and cyclothymic disorder; and anxiety disorders, such as panic disorder with or without agoraphobia, agoraphobia without history of panic disorder, specific phobias, for example, specific animal phobias, social phobias, obsessive-compulsive disorder, stress disorders including post-traumatic stress disorder and acute stress disorder, and generalised anxiety disorders.

The compounds of the present invention are also particularly useful in the treatment of nociception and pain. Diseases and conditions in which pain predominates, include soft tissue and peripheral damage, such as acute trauma, osteoarthritis, rheumatoid arthritis, musculo-skeletal pain, particularly after trauma, spinal pain, myofascial pain syndromes, headache, migraine, episiotomy pain, and burns.

The compounds of the present invention are also particularly useful in the treatment of respiratory diseases, particularly those associated with excess mucus secretion, such as chronic obstructive airways disease, bronchopneumonia, chronic bronchitis, cystic fibrosis and asthma, adult respiratory distress syndrome, and bronchospasm; in the treatment of inflammatory diseases such as inflammatory bowel disease, psoriasis, fibrositis, osteoarthritis, rheumatoid arthritis, pruritus and sunburn; and in the treatment of allergic disorders such as eczema and rhinitis. The compounds of the present invention are also particularly useful in the treatment of gastrointestinal (Gl) disorders, including inflammatory disorders and diseases of the Gl tract such as ulcerative colitis, Crohn's disease and irritable bowel syndrome.

The compounds of the present invention are also particularly useful in the treatment of emesis, including acute, delayed or anticipatory emesis, such as emesis induced by chemotherapy, radiation, toxins, pregnancy, vestibular disorders, motion, surgery, migraine, and variations in intercranial pressure. Most especially, the compounds of formula (I) are of use in the treatment of emesis induced by antineoplastic (cytotoxic) agents, including those routinely used in cancer chemotherapy; by radiation including radiation therapy such as in the treatment of cancer; and in the treatment of post-operative nausea and vomiting.

The excellent pharmacological profile of the compounds of the present invention offers the opportunity for their use in therapy at low doses thereby minimising the risk of unwanted side effects.

In the treatment of the conditions associated with an excess of tachykinins, a suitable dosage level is about 0.001 to 50 mg/kg per day, in particular about 0.01 to about 25 mg/kg, such as from about 0.05 to about 10 mg/kg per day.

For example, in the treatment of conditions involving the neurotransmission of pain sensations, a suitable dosage level is about 0.001 to 25 mg/kg per day, preferably about 0.005 to 10 mg/kg per day, and especially about 0.005 to 5 mg/kg per day. The compounds may be administered on a regimen of 1 to 4 times per day, preferably once or twice per day.

In the treatment of emesis, a suitable dosage level is about 0.001 to 10 mg/kg per day, preferably about 0.005 to 5 mg/kg per day, and especially 0.01 to 3 mg/kg per day. The compounds may be administered on a regimen of 1 to 4 times per day, preferably once or twice per day.

In the treatment of psychiatric disorders, a suitable dosage level is about 0.001 to 10 mg/kg per day, preferably about 0.005 to 5 mg/kg per day, and especially 0.01 to 3 mg/kg per day. The compounds may be administered on a regimen of 1 to 4 times per day, preferably once or twice per day.

It will be appreciated that the amount of a compound of formula (I) required for use in any treatment will vary not only with the particular compounds or composition selected but also with the route of administration, the nature of the condition being treated, and the age and condition of the patient, and will ultimately be at the discretion of the attendant physician.

As used herein, the term "treatment" includes prophylactic use to prevent the occurrence or recurrence of any of the aforementioned conditions.

According to a general process (Aa), compounds of formula (la) may be prepared by the reaction of a compound of formula (Ha)

wherein LG is a suitable leaving group such as an alkyl- or arylsulfonyloxy group (e.g. mesylate or tosylate) or a halogen atom (e.g. bromine, chlorine or iodine); by reaction with an amine of formula (Hla)

The reaction is preferably effected in the presence of a base, for example, an alkali metal carbonate, such as potassium carbonate, in a suitable organic solvent such as acetonitrile. The reaction ideally is performed at an elevated temperature, for example, between 40°C and 80°C, especially between 50°C and 70°C.

A particularly preferred compound of formula (Ha) is that wherein the group LG is mesylate, i.e. the group -OSO₂CH₃, which compound is prepared by reaction of the corresponding methanol with methanesulfonyl chloride in the presence of a base, such as triethylamine, and optionally a catalyst such as 4-N,N-dimethylaminopyridine. The reaction is conveniently effected in a solvent such as a halogenated hydrocarbon, for example, dichloromethane.

Using general process (Aa), compounds of formula (lab) may be prepared by the reaction of a compound of formula (Hah)

wherein LG is a suitable leaving group such as an alkyl- or arylsulfonyloxy group (e.g. mesylate or tosylate) or a halogen atom (e.g. bromine, chlorine or iodine); by reaction with an amine of formula (Hlab)

(Illab)

A particularly preferred compound of formula (Ilab) is that wherein the group LG is mesylate, i.e. the group -OSO₂CH₃, which compound is prepared by reaction of the corresponding methanol with methanesulfonyl chloride in the presence of a base, such as triethylamine, and optionally a catalyst such as 4-N,N-chmethylaminopyridine. The reaction is conveniently effected in a solvent such as a halogenated hydrocarbon, for example, dichloromethane.

According to another general process (Ba), compounds of formula (la) may be prepared by the reaction of an amine of formula QTfa) with a compound of formula (TVa)

in the presence of a reducing agent, for example, sodium triacetoxyborohydride or sodium cyanoborohydride. The reaction is conveniently effected in a suitable solvent such as a halogenated hydrocarbon, for example, 1,2-dichloroethane, conveniently at about room temperature.

Further details of suitable procedures will be found in the accompanying Examples.

Using general process (Ba), compounds of formula (lab) may be prepared by the reaction of an amine of formula (Hlab) with a compound of formula (INab)

Further details of suitable procedures will be found in the accompanying Examples. Compounds of formulae (Ha) and (Ilab) may be prepared by conventional methods such as those described in International (PCT) Patent Publication No. WO 00/56727.

Compounds of formula (Hla), and compounds of formula (Hlab) wherein X is CHOH, may be prepared from a suitably substituted precursor piperidine compound, for instance, as shown in Scheme 1 (in which R is, for example, a Chalky! group, such as an ethyl group, and P is an amino protecting group, such as a benzyloxycarbonyl group):

Scheme 1

LiBH₄, THF TBAF, THF

An alternative (asymmetric) synthesis of compounds of formula (Dla), and compounds of formula (IHab) wherein X is CHOH, is shown in Scheme 2: Scheme 2

LDA or KHMDS

O_cor cat. OsOJIO.

H₂O

Hydroboration

A further alternative (asymmetric) synthesis of compounds of formula (Hla), and compounds of formula (Dlab) wherein X is CHOH, is shown in Scheme 3:

Scheme 3

LiBH,

It will be appreciated that numerous other routes will be readily apparent to one of ordinary skill in the art, the route of choice depending upon the particular spiro-amine that is desired. The methodology described herein may be adapted accordingly. Thus, for instance, in addition to the processes outlined in Schemes 1, 2 and 3 above, amines of formula (DTa) may be prepared by other methods that would be readily apparent to one of ordinary skill in the art. For example, methods for the preparation of the spirocyclic amines are provided in the literature - see for instance, Bioorg. Med. Chem. Lett., 12 (2002) 1759-62 and International (PCT) Patent Publication No. WO 01/87838.

Compounds of formulae (IVa) and (IVab) may be prepared by conventional methods such as those described in International (PCT) Patent Publication No. WO 00/56727, for instance, by the oxidation of a corresponding vinyl compound using oxygen and ozone. The reaction is conveniently effected at a reduced temperature, such as at about -78°C, in the presence of an organic solvent such as an alcohol, for example, methanol, or a halogenated hydrocarbon, for example, dichloromethane, or a mixture thereof. Treatment with dimethylsulfide liberates the desired aldehyde.

According to a general process (Ab), compounds of formula (lb) in which m is zero and n is 2 or 3 may be prepared by the cyclization of a compound of formula (Hb) in the presence of an amine of the formula R¹⁰R^πNH:

wherein LG¹ and LG² are independently leaving groups, such as mesylate, tosylate, brosylate, nosylate or triflate. Conveniently, LG¹ and LG² may be the same or different, for instance LG¹ may be mesylate whilst LG² may be tosylate.

The reaction is effected at an elevated temperature, for example, between 120° and 160°C, preferably at about 140°C, in a suitable solvent, such as an alcohol, for instance, methanol.

According to another general process (Bb), compounds of formula (lb) in which m is 1 and n is 1 or 2 may be prepared by the cyclization of a compound of formula (IJTo) in the presence of an amine of the formula R¹⁰R^πNH:

wherein LG¹ and LG² are as previously defined.

According to another general process (Cb), compounds of formula (lb) in which m is 2 and n is 2 may be prepared by the cyclization of a compound of formula (INb) in the presence of an amine of the formula R¹⁰R^UΝH:

wherein LG¹ and LG² are as previously defined.

Further details of suitable procedures will be found in the accompanying Examples. Compounds of formula (Hb) may be prepared from a compound of formula (Nb)

by reaction with a dihydroxylating agent such as AD-mix-α or AD-mix-β (see Aldrichimica Acta 1994, 27, 70), followed by conversion of the primary alcohol to the LG² group.

The dihydroxylation reaction is conveniently effected at room temperature in a solvent such as an alcohol, for instance, tert-butanol, or water, or more preferably a mixture thereof.

The conversion of the primary alcohol to the LG group is effected in a conventional manner, for instance by reaction with the appropriate sulfonyl chloride in the presence of a base, such as triethylamine. The reaction is conveniently effected in a solvent such as a halogenated hydrocarbon, for example, dichloromethane, at a reduced temperature, such as below -20°C, and then at room temperature. Addition of dibutyltin oxide to the reaction conveniently promotes formation of the leaving group on the primary alcohol group.

Similarly, compounds of formula (Hib) may be prepared from a compound of formula (Nib)

Likewise, compounds of formula (IVb) may be prepared from a compound of formula (NHb)

Compounds of formula (Nb) may be prepared by reaction of an aldehyde of formula (NUTo)

(Villb) with a suitable Grignard reagent, such as CH₂=CH(CH₂)_nCH₂MgBr or CH₂=CH(CH₂)_nCH₂MgCl, followed by conversion of the secondary alcohol to the LG¹ group.

The Grignard reaction is effected in the presence of a suitable solvent such as an ether, for instance, tetrahydrofuran, at a reduced temperature, for example, below - 40°C, and preferably at about -78°C.

The conversion of the secondary alcohol to the LG¹ group is effected in a conventional manner, for instance by reaction with the appropriate sulfonyl chloride in the presence of a base, such as triethylamine. The reaction is conveniently effected in a solvent such as a halogenated hydrocarbon, for example, dichloromethane, at a reduced temperature, such as below -20°C, and then at room temperature.

Compounds of formula (Nπib) may be prepared by conventional methods such as those described in International (PCT) Patent Publication No. WO 00/56727, for instance, by the oxidation of a corresponding vinyl compound using oxygen and ozone. The reaction is conveniently effected at a reduced temperature, such as at about -78°C, in the presence of an organic solvent such as an alcohol, for example, methanol, or a halogenated hydrocarbon, for example, dichloromethane, or a mixture thereof. Treatment with dimethylsulfide liberates the desired aldehyde.

Compounds of formula (Nib) may be prepared from a compound of formula (Kb)

by reaction with a reducing agent such as a borohydride, for example, sodium borohydride, followed by conversion of the alcohol thus formed into the LG¹ group as previously described. The reduction is effected in a suitable solvent such as an alcohol, for example, ethanol, at a reduced temperature such as between -10°C and +5°C, preferably at about 0°C.

Similarly, compounds of formula (VHb) may be prepared from a compound of formula (Xb)

Compounds of formula (IXb) may be prepared from a compound of formula (Xlb)

by reaction with, for example, an ylide such as that prepared from Ph3P⁺CH₂OMeCl^" in the presence of a strong base such as lithium diisopropylamide and a suitable solvent such as an ether, for example, tetrahydrofuran, followed by treatment with an acid such as a mineral acid, for example, aqueous hydrochloric acid.

Compounds of formula (Xlb) may be prepared from a compound of formula (Xllb)

by a suitable oxidation reaction, such as a Swern oxidation using dimethylsulfoxide and oxalyl chloride, and a base such as triethylamine. The reaction is conveniently effected in a solvent such as a halogenated hydrocarbon, for example, dichloromethane, at a reduced temperature, for example at about -78°C, and then warming to room temperature.

Compounds of formula (XHb) may be prepared from a compound of formula (Nπib) by reaction with a suitable Grignard reagent as previously described.

Compounds of formula (Xb) may be prepared from a compound of formula (LXb) under the conditions described above for the preparation of a compound of formula (IXb).

According to a general process (Ac), compounds of formula (Ic) in which A is

ΝR 19 and B is S(O)_q (where q is zero) may be prepared by the reaction of a compound

19 of formula (Ee) in the presence of an amine of the formula R ΝH₂:

wherein LG and LG are independently leaving groups, such as mesylate, tosylate,

1 9 brosylate, nosylate or triflate. Conveniently, LG and LG may be the same or different, for instance LG¹ is may be mesylate whilst LG² may be tosylate.

According to another general process (Be), compounds of formula (Ic) in which A is S(O)_q (where q is zero) and B is NR¹² may be prepared from a compound of formula (ITIc)

wherein LG¹ and LG² are as previously defined, and P is an amine protecting group, by reaction with sodium sulfide, followed, if desired, by deprotection of the amine and subsequent alkylation of the amine to vary the group R¹².

The cyclization reaction is effected in a suitable solvent such as acetone at an elevated temperature, for example, at the reflux temperature of the solvent.

A particularly suitable amine protecting group is an alkoxycarbonyl group such as tert-butoxycarbonyl, which is conveniently removed by treatment with trifluoroacetic acid in a solvent such as a halogenated hydrocarbon, for example, dichloromethane, at a reduced temperature, such as between -10°C and +5°C, for example, at about 0°C.

Further details of suitable procedures will be found in the accompanying Examples. Compounds of formula (He) may be prepared from a corresponding diol of formula (INc)

The conversion of the alcohols to the LG¹ and LG² groups is effected in a conventional manner, for instance by reaction with the appropriate sulfonyl chloride in the presence of a base, such as triethylamine. The reaction is conveniently effected in a solvent such as a halogenated hydrocarbon, for example, dichloromethane, at a reduced temperature, such as below -20°C, and then at room temperature.

Similarly, compounds of formula (Hie) may be prepared from a corresponding diol of formula (Nc)

Compounds of formula (INc) may be prepared from an epoxide of formula (NIc)

by reaction with HOCH₂CH₂SH in the presence of a base such as a hydroxide, for instance, potassium hydroxide. The reaction is conveniently effected in a solvent such as an alcohol, for example isopropanol, at an elevated temperature, for example at the reflux temperature of the solvent.

Compounds of formula (Vc) may be prepared from an epoxide of formula (Vic) by reaction with HOCH₂CH₂NH₂ in a solvent such as an alcohol, for example methanol, at an elevated temperature, for example at the reflux temperature of the solvent, followed by protection of the secondary amine, for example with di-tert-butyl dicarbonate. This reaction is conveniently effected in a solvent such as a halogenated hydrocarbon, for example, dichloromethane, at room temperature.

Compounds of formula (Vic) may be prepared from a compound of formula (VIIc)

by reaction with trimethylsulfonium iodide in the presence of a base such as sodium hydride. The reaction is conveniently effected in a suitable solvent such as dimethylsulfoxide or an ether, such as tetrahydrofuran, or a mixture thereof, at a reduced temperature between -10°C and +5°C, for example, at about 0°C. Compounds of formula (VIIc) may be prepared by conventional methods such as those described in International (PCT) Patent Publication No. WO 00/56727, for instance, by the oxidation of a corresponding vinyl compound of formula (VDIc)

(Vlllc)

using oxygen and ozone. The reaction is conveniently effected at a reduced temperature, such as at about -78°C, in the presence of an organic solvent such as an alcohol, for example, methanol, or a halogenated hydrocarbon, for example, dichloromethane, or a mixture thereof. Treatment with dimethylsulfide liberates the desired aldehyde.

In an alternative method, compounds of formula (Vie) may be prepared directly from a compound of formula (VIHc) by an epoxidization reaction using a peracid, for example, m-chloroperbenzoic acid. The reaction is effected in a solvent such as a halogenated hydrocarbon, for example, dichloromethane, conveniently at room temperature.

Compounds of formula (VIHc) are known compounds or may be prepared by conventional methods such as those described in International (PCT) Patent Publication Nos. WO 00/56727 and WO 02/16344.

According to a general process (Ad), compounds of formula (Id) in which m is zero and n is 1 may be prepared by the cyclization of a compound of formula (Ed) in the presence of an amine of the formula R^πNH₂:

1 9 wherein LG and LG are independently leaving groups, such as mesylate, tosylate, brosylate, nosylate or triflate. Conveniently, LG¹ and LG² may be the same or different, for instance LG¹ and LG² may both be mesylate.

According to another general process (Bd), compounds of formula (Id) in which m is 1 or 2 and n is zero may be prepared by the cyclization of a compound of formula ( ld) in the presence of an amine of the formula R lb NH₂:

wherein LG¹ and LG² are as previously defined.

The reaction is effected at an elevated temperature, for example, between 120° and 160°C, preferably at about 140°C, in a suitable solvent, such as an alcohol, for instance, methanol. According to another general process (Cd), compounds of formula (Id) in which m is 2 and n is 1 may be prepared by the cyclization of a compound of formula (INd) in the presence of an amine of the formula R ΝH :

1 9 wherein LG and LG are as previously defined.

The reaction is effected at an elevated temperature, for example, between 120° and 160°C, preferably at about 130°C, in a suitable solvent, such as an alcohol, for instance, methanol.

With regard to general processes (Bd) and (Cd), it will be appreciated by one of ordinary skill in the art that the geminal hydroxyl group can be removed using conventional methodology.

Compounds of formula (Ed) may be prepared from a compound of formula (Vd)

The conversion of the primary and secondary alcohols to the LG¹ and LG² groups is effected in a conventional manner, for instance by reaction with the appropriate sulfonyl chloride (e.g. methanesulfonyl chloride) in the presence of a base, such as triethylamine. The reaction is conveniently effected in a solvent such as a halogenated hydrocarbon, for example, dichloromethane, at a reduced temperature, such as below -20°C, and then at room temperature.

Similarly, compounds of formula (Eld) may be prepared from a compound of formula (VId)

Likewise, compounds of formula (IVd) may be prepared from a compound of formula (VEd)

Compounds of formula (Vd) may be prepared from a compound of formula (VIEd)

(Vllld)

by reaction with ozone, followed by treatment with sodium borohydride.

The ozonolysis reaction is effected at a reduced temperature, for example, below -40°C, and preferably at about -78°C, in a suitable solvent such as a halogenated hydrocarbon, for example, dichloromethane, or an alcohol, for example, methanol, or a mixture thereof.

Similarly, compounds of formula (VId) may be prepared from a compound of formula (IXd)

where Q is -CH= (for compounds where m is 1) or -CH₂CH= (for compounds where m is 2).

Likewise, compounds of formula (VEd) may be prepared from a compound of formula (Xd)

Compounds of formula (NLEd) may be prepared by reaction of a compound of formula (Xld)

with a suitable Grignard reagent, such as CH₂=CHCH₂MgBr or CH₂=CHCH₂MgCl. The reaction is effected in the presence of a suitable solvent such as an ether, for instance, diethyl ether or tetrahydrofuran, at a reduced temperature, for example, below -40°C, and preferably at about -78°C.

The stereochemistry of the intermediate of formula (VIEd) may be directed by the inclusion in the Grignard reaction of chlorobis[(15,2R,35',55')-2,6,6- trimethylbicyclo[3.1.1]hept-3-yl]borane ((+)-DIP-Cl) or chlorobis[(lR,2S,3R,5R)- 2,6,6-trimethylbicyclo[3.1.1]hept-3-yl]borane ((-)-DIP-Cl) which is conveniently pre- mixed with the allyl Grignard reagent at a reduced temperature, for instance, at about - 40°C, and warmed to about room temperature, before cooling and addition to the aldehyde of formula (Xld).

Compounds of formula (Xld) may be prepared by conventional methods such as those described in International (PCT) Patent Publication No. WO 00/56727, for instance, by the oxidation of a corresponding vinyl compound using oxygen and ozone. The reaction is conveniently effected at a reduced temperature, such as at about -78°C, in the presence of an organic solvent such as an alcohol, for example, methanol, or a halogenated hydrocarbon, for example, dichloromethane, or a mixture thereof. Treatment with dimethylsulfide liberates the desired aldehyde.

Compounds of formulae (TXd) where Q is -CH= and (Xd) may be prepared from a compound of formula (XEd)

by reaction with a suitable Grignard reagent, for instance, of the formula CH₂=CHMgBr (for formula (IXd) where Q is -CH=) or CH₂=CHCH₂MgBr (for formula (Xd)). The reaction is effected in the presence of a suitable solvent such as an ether, for instance, diethyl ether or tetrahydrofuran, at a reduced temperature, for example, below -40°C, and preferably at about -78°C. Cerium (ET) chloride may be added to avoid reduction and enolization side reactions.

Compounds of formula (XEd) may be prepared from the corresponding carboxylic acid by conventional procedures, for instance, by reaction of the carboxylic acid with (trimethylsilyl)diazomethane and methanol in a suitable solvent such as toluene, conveniently at room temperature.

Compounds of formula (IXd) where Q is -CH₂CH= may be prepared from a compound of formula (XEEd)

by reaction with a suitable Grignard reagent, such as CH₂=CHMgBr or CH₂=CHMgCl, as previously described.

Compounds of formula (XEId) may be prepared from a compound of formula (XINd)

Compounds of formula (XlVd) may be prepared from a compound of formula (Xld) by reaction with a suitable Grignard reagent, such as CH₂=CHCH₂MgBr or CH₂=CHCH₂MgCl, as previously described. According to a general process (Ae), compounds of formula (le) may be prepared by the reaction of a compound of formula (Ee)

with an amine of the formula R 13 Rr> 14 N* H,

The reaction is effected at an elevated temperature, for example, between 110° and 150°C, preferably at about 130°C, in a suitable solvent, such as an alcohol, for instance, methanol.

Compounds of formula (Ee) may be prepared from a compound of formula (Ble)

by reaction with trimethylsulfonium iodide in the presence of a base such as sodium hydride. The reaction is conveniently effected in a suitable solvent such as dimethylsulfoxide or an ether, such as tetrahydrofuran, or a mixture thereof, at a reduced temperature between -10°C and +5°C, for example, at about 0°C.

Compounds of formula (TEe) may be prepared by conventional methods such as those described in International (PCT) Patent Publication No. WO 00/56727, for instance, by the oxidation of a corresponding vinyl compound using oxygen and ozone. The reaction is conveniently effected at a reduced temperature, such as at about -78°C, in the presence of an organic solvent such as an alcohol, for example, methanol, or a halogenated hydrocarbon, for example, dichloromethane, or a mixture thereof. Treatment with dimethylsulfide liberates the desired aldehyde.

Amines of use in the general process (Ae) are either known compounds, or may be prepared by methods well known to one of ordinary skill in the art. For example, methods for the preparation of the spirocyclic amines are provided in the literature - see for instance, Bioorg. Med. Chem. Lett., 12 (2002) 1759-62 and International (PCT) Patent Publication No. WO 01/87838.

According to a general process (Af), compounds of formula (If) wherein R^π represents CO₂C_1-6alkyl and R¹² represents OH, may be prepared by the treatment of a compound of the formula (Ef)

with a lithiating agent such as an alkyl lithium compound, for example, .sec-butyllithium in the presence of an organic base such as N,N,N',N'-tetramethyl- 1,2-ethanediamine, in a suitable solvent, such as an ether, for example, diethyl ether, at a reduced temperature, such as below -40°, and preferably at about -78°C; which treatment is followed by reaction with a compound of formula (Iflf)

The reaction is conveniently effected in a suitable solvent, such as an ether, for example, diethyl ether, at a reduced temperature, such as below -40°, and preferably at about -78°C.

According to another general process (Bf), compounds of formula (If) wherein

19 1 1 19 1 1

R is linked to R such that there is formed a ring, the linkage -R -R - being -OC(O)-, may be prepared by the interconversion of a compound of formula (If) wherein R¹¹ represents CO₂C_1-6alkyl and R¹² represents OH by treatment with a reducing agent such as a hydride, for instance sodium hydride, the reaction being effected in a suitable solvent such as an ether, for example, tetrahydrofuran, conveniently at about room temperature.

According to another general process (Cf), compounds of formula (If) wherein

1 1 19

R represents hydrogen and R represents OH (which compounds may themselves serve as precursors for further derivatization of the amino moiety, may be prepared by the interconversion of a compound of formula (If) wherein R¹² is linked to R¹¹ such that there is formed a ring, the linkage -R¹²-R^u- being -OC(O)-, by treatment with lithium hydroxide, the reaction being effected in a suitable solvent such as an ether, for example, tetrahydrofuran, and alcohol, for example, methanol, or water, or a mixture thereof, at an elevated temperature, such as between 40°C and 60°C, preferably at about 50°C.

Compounds of formula (Ef) are either known compounds or they may be prepared by methods readily apparent to one of ordinary skill in the art- Compounds of formula (TEf) may be prepared by conventional methods such as those described in International (PCT) Patent Publication No. WO 00/56727, for instance, by the oxidation of a corresponding vinyl compound using oxygen and ozone. The reaction is conveniently effected at a reduced temperature, such as at about -78°C, in the presence of an organic solvent such as an alcohol, for example, methanol, or a halogenated hydrocarbon, for example, dichloromethane, or a mixture thereof. Treatment with dimethylsulfide liberates the desired aldehyde.

It will be appreciated that the general methodology described above may be adapted, using methods that are readily apparent to one of ordinary skill in the art, in order to prepare further compounds of the present invention. In particular, compounds of formula (I) may be interconverted into further compounds of formula (I) using conventional synthetic techniques such as N-alkylation or O-alkylation, oxidation or reduction.

Thus, for instance, a compound of formula (la), (lab) or (lb) wherein X is CHOH may be converted into a corresponding compound of formula (la), (lab) or (lb) respectively wherein X is C=O by reaction with a strong oxidizing agent, such as Dess-Martin periodinane, to afford the desired ketone.

Also, for instance, for the compounds of formula (Ic), the NR¹² moiety where R¹² is hydrogen may be alkylated by reaction with a suitable alkyl halide, such as methyl iodide or ethyl iodide, conveniently at about room temperature, in the presence of an acid acceptor such as potassium carbonate or N,N-diisopropylethylamine.

In another example of such interconversion reactions, the sulfur atom of the thiomorpholine ring of the compounds of formula (Ic) may be oxidized using, for instance, sodium metaperiodate in acetic acid to afford the corresponding sulfoxide or sulfone.

Also, for instance, the compounds of formula (Id) having the NR¹¹ moiety where R¹¹ is hydrogen and NH moieties in the compounds of formulae (le) and (If) may be alkylated by reaction with a suitable alkyl halide, such as methyl iodide or ethyl iodide, conveniently at about room temperature, in the presence of an acid acceptor such as potassium carbonate or N,N-diisopropylethylamine.

In another example of such interconversion reactions, a compound of formula (le) or (If) wherein Z is S may be converted into a corresponding compound of formula (le) or (If) respectively wherein Z is SO or S(O)₂ by an oxidation reaction using, for instance, sodium metaperiodate in acetic acid to afford the desired sulfoxide or sulfone. Other general methods that may be adapted to the synthesis of the pyran acetal derivatives of the present invention are described in International (PCT) Patent Publication Nos. WO 00/56727 and WO 02/16344.

During any of the above synthetic sequences it may be necessary and/or desirable to protect sensitive or reactive groups on any of the molecules concerned. This may be achieved by means of conventional protecting groups, such as those described in Protective Groups in Organic Chemistty, ed. J.F.W. McOmie, Plenum Press, 1973; and T.W. Greene and P.G.M. Wuts, Protective Groups in Organic Synthesis, John Wiley & Sons, 1991. The protecting groups may be removed at a convenient subsequent stage using methods known from the art.

The exemplified compounds of this invention were tested by the methods set out at pages 36 to 39 of International Patent Specification No. WO 93/01165. The compounds were found to be active with IC₅₀ at the NKi receptor of less than lOOnM on said test method.

The following non-limiting Examples serve to illustrate the preparation of compounds of the present invention:

DESCRIPTION 1 Piperidine-l,4-dicarboxylic acid 1-benzyl ester 4-ethyl ester

A solution of benzyl chloroformate (95g, 0.56mol) in dichloromethane (200ml) was added dropwise to an ice-bath-cooled, stirred mixture of ethyl isonipecotate (87g, 0.55mol), sodium carbonate (60g, 0.57mol) and dichloromethane (200ml) over 70 minutes. The mixture was stirred at ambient temperature for 2.5 days and filtered though a pad of Celite™. The filtrate was concentrated in vacuo. The residue was partitioned between 2M aqueous hydrochloric acid and diethyl ether. Organic layer was separated, dried (MgSO ), filtered and concentrated. The residue was chromatographed on silica gel (ethyl acetate/iso-hexane) to give the title product (152g, 94%).

1H NMR (360 MHz, CDC1₃): δ 7.41-7.27 (5H, m), 5.12 (2H, s), 4.22-3.99 (2H, m), 4.14 (2H, q, /7.4Hz), 2.93 (2H, br t, / 11.6Hz), 2.45 (2H, m), 1.97-1.81 (2H, m), 1.74-1.56 (2H, m), 1.25 (3H, t, ./7.4Hz). DESCRIPTION 2 4-[2-(fe/ -ButyldimethyIsiIyloxy)-l-hydroxyethyI]-piperidine-l,4-dicarboxyIic acid 1-benzyl ester 4-ethyl ester

A solution of piperidine-l,4-dicarboxylic acid 1 -benzyl ester 4-ethyl ester (Description 1; lOg, 34.4rnmol) in tetrahydrofuran (100ml) was cooled to -78°C. Lithium bis(trimethylsilyl)amide (l.OM in tetrahydrofuran, 50ml, 50mmol) was added dropwise and the mixture stirred, allowing to warm to room temperature over 1.5 hours. The orange solution was re-cooled to -78°C and {tert- butyldimethylsilyloxy)acetaldehyde (lOg, 57.3mmol) added. The mixture was allowed to warm to room temperature over 2.5 hours, then quenched with saturated aqueous ammonium chloride solution. The reaction mixture was partitioned between diethyl ether and water. The organic layer was dried (MgSO₄) and concentrated in vacuo. The residue was purified by flash column chromatography on silica, eluting with 5 -15% diethyl ether/dichloromethane, to give the title compound (12.4g, 78%). 1H NMR (400 MHz, CDC1₃): δ 7.36-7.35 (5H, m), 5.30 (2H, s), 4.21 (2H, q, J 7.1Hz), 4.15-4.04 (2H, m), 3.66-3.57 (3H, m), 2.83 (2H, d, J4.3Hz), 2.23-2.05 (2H, m), 1.63- 1.56 (2H, m), 1.29 (3H, t, /7.1Hz), 0.88 (9H, s), 0.06 (6H, s).

DESCRIPTION 3 4-(l,2-Dihydroxyethyl)-4-hydroxymethyI-piperidine-l-carboxylic acid benzyl ester

Lithium borohydride (lg, 45mmol) was added portionwise to a solution of 4-[2-(tert- butyldimefhylsilyloxy)- 1 -hydroxyethyl] -piperidine- 1 ,4-dicarboxylic acid 1 -benzyl ester 4-ethyl ester (Description 2; 11.2g, 24.1mmol) in tetrahydrofuran (75ml). The mixture was stirred at room temperature for 2 hours. The reaction was quenched by addition of saturated aqueous sodium hydrogencarbonate solution, then partitioned between ethyl acetate and water. The organic layer was dried (MgSO ) and concentrated in vacuo to give the diol. This was dissolved in tetrahydrofuran (50ml). Tetrabutylammonium fluoride (l.OM in tetrahydrofuran, 32ml, 32mmol) was added and the reaction stirred at room temperature for 1 hour. The mixture was partitioned between water and ethyl acetate. The organic layer was dried (MgSO₄) and concentrated in vacuo. The residue was purified by flash column chromatography on silica, eluting with 1, 2, 5 and 10% methanol/ethyl acetate, to give the title compound (5.4g, 73%).

1H NMR (360 MHz, CDC1₃): δ 7.39-7.29 (5H, m), 5.13 (2H, s), 3.82-3.70 (6H, m), 3.54 (IH, t, 74.7Hz), 3.26-2.74 (5H, m), 1.73-1.54 (3H, m), 1.39-1.31 (IH, m).

DESCRIPTION 4

4-Hydrosy-2-osa-§-asa-spiro[4.5]decane-8-carbosylk acid bensyl ester Diethyl azodicarboxylate (1.4g, S.lmmol) was added to a solution of 4-(l,2- dihydroxyethyl)-4-hydroxymethyl-piperidine-l -carboxylic acid benzyl ester (Description 3; 2g, 6.5mmol) and triphenylphospliine (2.4g, 9.1mmol) in tetrahydrofuran (40ml). The reaction was stirred at room temperature for 2 hours then the solvent was removed in vacuo. The residue was purified by flash column chromatography on silica, eluting with 1, 2 and 4% methanol / dichloromethane, to give the title compound (1.43g, 76%).

1H NMR (400 MHz, CDC1₃): δ 7.38-7.30 (5H, m), 5.13 (2H, s), 4.12-4.08 (IH, m), 4.02-3.99 (IH, m), 3.74-3.65 (5H, m), 3.39-3.33 (IH, m), 3.27-3.20 (IH, m), 1.89- 1.87 (IH, m), 1.82-1.76 (IH, m), 1.59-1.54 (IH, m), 1.51-1.48 (2H, m).

DESCRIPTION 5 4-[2-(før^-Butyldimethylsilyloxy)-l-hydroxyethyl]-piperidine-4-dicarboxylic acid l-(l,l-dimethylethyl) ester 4-ethyl ester

The title compound was prepared as described in Description 2 from piperidine-1,4- dicarboxylic acid 1 -tert-butyl ester 4-ethyl ester.

1H NMR (360 MHz, CDC1₃): δ 4.21 (2H, q, /7.0Hz), 4.08-3.90 (2H, m), 3.75-3.50 (3H, m), 2.89-2.69 (2H, m), 2.19 (IH, m), 2.03 (IH, m), 1.57 (2H, dt, /4.6, 13.0Hz), 1.45 (9H, s), 1.29 (3H, t, 77.4Hz), 0.89 (9H, s), 0.06 (6H, s).

DESCRIPTION 6 4-(l,2-Dihydrosyethyl)-4-hydrosymethyl-piperidine-l-carbo-syl cid 1,1- dimethylethyl ester

The title compound was prepared as described in Description 3 from the product of Description 5. 1H NMR (400 MHz, CDC1₃): δ 3.80-3.60 (6H, m), 3.55 (IH, m), 3.24-3.02 (3H, m), 1.73-1.48 (4H, m), 1.45 (9H, s), 1.33 (IH, m).

DESCRIPTION 7 4-Hydro-sy-2-o-∑a-8-a_.a-spiro[4.S]decane-8-carbθ-sylic aci 1,1-dimethylethyl ester

The title compound was prepared as described in Description 4 from the product of

Description 6.

1H NMR(400 MHz, CDC1₃): δ 4.11 (IH, dd, 74.7, 10.2Hz), 4.01 (IH, dt, 71.6,

4.7Hz), 3.74 (IH, d, 78.6Hz), 3.73 (IH, dd, 72.0, 10.7Hz), 3.67 (IH, d, 78.6Hz),

3.63 (2H, m), 3.27 (IH, dt, 76.3, 13.7Hz), 3.14 (IH, ddd, 73.5, 9.0, 12.9Hz), 1.82

(IH, d, 5:5Hz), 1.77 (IH, ddd, 73.9, 9.0, 13.3Hz), 1.56 (IH, ddd, 73.5, 6.3,

13.3Hz), 1.50-1.45 (2H, m), 1.46 (9H, s).

DESCRIPTION 8 (R5)-2-Oxa-8-aza-spiro[4.5]decan-4-ol hydrochloride

4-Hydroxy-2-oxa-8-aza-spiro[4.5]decane-8-carboxylic acid 1,1-dimethylethyl ester

(Description 7; 0.86g, 3.3mmol) was stirred overnight in 2M ethereal hydrogen chloride. The mixture was filtered and the solid dried to give the title compound

(0.5g, 77%).

1H NMR (360 MHz, MeOH- ): δ 4.15-4.06 (2H, m), 3.71-3.61 (3H, m), 3.69-3.13

(4H, m), 2.08-2.03 (IH, m), 1.80-1.74 (3H, m).

DESCRIPTION 9

(4R)-4-[(15,4R)-4,7,7-Trimethyl-3-oxo-2-oxa-bicyclo[2.2.1]heptane-l- carbonyloxy]-2-oxa-8-aza-spiro[4.5]decane-8-carboxylic acid benzyl ester and (4S)-4-[(lS',4R)-4,7,7-Trimethyl-3-oxo-2-oxa-bicyclo[2.2.1]heptane-l- carbonyløι_y]-2-oxa-8-asa-spiro[4.5]decanβ-8-carbøxyIk acid foensyl ester (l$)-(-)-Camphanic chloride (335mg, 1.54mmol) was added to a stirred solution of 4- hydroxy-2-oxa-8-aza-spiro[4.5]decane-8-carboxylic acid benzyl ester (Description 4; 300mg, 1.03 mmol), triethylamine (0.4ml, 2.9mmol) and

4-N,N-dimethylaminopyridine (15mg, 0.12mmol) in dichloromethane (5ml). The mixture was stirred overnight and diluted with ethyl acetate and washed with saturated aqueous NaHCO₃, 2M HCI and water. The organic phase was dried (Na₂SO₄) and concentrated. The residue was purified on silica (i-hexane:ethyl acetate) to give the title ester as a mixture of diastereoisomers (460mg). Diastereoisomers were separated on prep. HPLC (HIRPB, acetonitrile: 0.1% aq. trifluoroacetic acid) to give isomer A (190mg) and isomer B (195mg).

Isomer A: (4S)-isomer of the title compound

1H NMR (400 MHz, CDC1₃): δ 7.41 (5H, m), 5.13 (2H, s), 5.11 (IH, dd, 7 1.6, 4.7Hz),

4.27 (IH, dd, 74.7, 11.3Hz), 3.87 (IH, d, 78.6Hz), 3.77 (IH, dd, 72.0, 11.3Hz), 3.77-

3.67 (2H, m), 3.67-3.58 (IH, m), 3.40 (IH, dt, 76.3, 14.1Hz), 3.21 (IH, m), 2.38 (IH, ddd, 74.3, 11.0, 13.7Hz), 2.05 (IH, dt, 74.7, 9.4, 13.7Hz), 1.94 (IH, ddd, 74.3, 10.6,

12.9Hz), 1.77-1.67 (2H, m), 1.66-1.56 (3H, m), 1.12 (3H, s), 1.07 (3H, s), 0.96 (3H, s).

Isomer B: (4R)-isomer of the title compound

1H NMR (400 MHz, CDC1₃): δ 7.40-7.29 (5H, m), 5.13 (2H, s), 5.11 (IH, dd, / 1.6, 4.7Hz), 4.27 (IH, dd, 75.1, 11.3Hz), 3.87 (IH, d, 78.6Hz), 3.77-3.66 (3H, m), 3.40 (IH, dt, 76.3, 14.4Hz), 3.21 (IH, m), 2.43 (IH, ddd, 74.3, 10.6, 13.3Hz), 2.01 (IH, ddd, 74.7, 9.0, 13.3Hz), 1.93 (IH, ddd, 74.3, 10.6, 12.9Hz), 1.80-1.65 (3H, m), 1.64- 1.56 (3H, m), 1.12 (3H, s), 1.04 (3H, s), 0.97 (3H, s).

DESCRIPTION 10 (15,4R)-4,7,7-Trimethyl-3-oxo-2-oxa-bkyclo[2.2.1]heptane-l-carboxylic acid (47?)-2-oxa-8-aza-spiro[4.5]dec-4-yl ester trifluoroacetate

A mixture of isomer B of the product of Description 9 (130mg, 0.27mmol), palladium on carbon (10%, 73mg), trifluoroacetic acid (0.1ml) and ethanol (20ml) was stirred at room temperature under H₂ atmosphere (balloon) for 30 minutes, and then filtered through a pad of Celite™ and concentrated to give the title compound (130mg). 1H NMR (400 MHz, MeOH-J₄): δ 5.19 (IH, dd, 72.0, 4.7Hz), 4.29 (IH, dd, 75.0, 10.8Hz), 3.86 (IH, d, 78.8Hz), 3.76 (IH, dd, 72.0, 11.1Hz), 3.69 (IH, d, 8.8Hz), 3.26-3.11 (3H, m), 3.03 (IH, ddd, 73.5, 8.8, 12.6Hz), 2.50 (IH, ddd, 76.1, 12.6, 15.2Hz), 2.09-1.90 (3H, m), 1.98-1.74 (3H, m), 1.63 (IH, m), 1.64-1.56 (3H, m), 1.10 (3H, s), 1.08 (3H, s), 0.97 (3H, s). DESCRIPTION 11

2-[l-{(2^,3fi,4fi)-2-[(LK)-l-(3,5-Bis(trifluoromethyl)phenyl)ethoxy]-3-(4- fluorophenyl)-tetrahydropyran-4-carbaldelιyde

N-Methylmorpholine N-oxide (Ig, 8.6mmol) and osmium tetroxide (2ml, 2.5% solution in fert-butanol) were added to a solution of (2R,3R,45)-2-[(lR)-l-(3,5- bis(trifluoromethyl)phenyl)ethoxy]-3-(4-fluorophenyl)-4-vinyl-tetrahydropyran (see Example 147 in WO 00/56727; 2g, 4.3mmol) in tetrahydrofuran (25ml) and water (10ml). The mixture was stirred at room temperature for one hour before sodium periodate (9g, 43mmol) was added. This mixture was stirred for 45 minutes and then filtered through Celite™. The filtrate was diluted with ethyl acetate (50ml) and washed with brine. The organic extracts were dried and concentrated. The residue was chromatographed on silica gel eluting with ethyl acetate / iso-hexanes to give the title compound (1.6g, 80%).

1H NMR (400 MHz, CDC1₃): δ 9.47 (IH, s), 7.70 (IH, s), 7.27 (2H, s), 7.15-7.12 (2H, m), 6.99-6.95 (2H, m), 4.97 (IH, q, 76.6Hz), 4.28-4.16 (2H, m), 3.60-3.58 (IH, m), 3.00 (IH, dd, /7.3, 10.6Hz), 2.80-2.70 (lH,m), 1.85-1.81 (2H, m), 1.39 (3H, d, / 6.6Hz).

DESCRIPTION 12 (lS,4R)-4,7,7-Trimethyl-3-oxo-2-oxa-bicyclo[2.2.1]heptane-l-carboxylic acid (45)-8-{(2R₅3R,4R)-2-[(lR)-l-(3,5-bis(trifluoromethyl)phenyl)ethoxy]-3-(4- fluorophenyl)-tetrahydropyran-4-ylmethyl}-2-oxa-8-aza-spiro[4.5]dec-4-yl ester

Palladium hydroxide (20% on carbon, 20mg) was added as a slurry in ethanol to a solution of isomer A of the product of Description 9 (120mg, 0.25mmol) in ethanol (5ml). The mixture was hydrogenated in a Parr apparatus (45 psi) for 30 minutes then filtered through Celite™ and washed with ethanol. The filtrate was concentrated in vacuo. The residue and 2-[l-{(2R,3R,4R)-2-[(lR)-l-(3,5- bis(trifluoromethyl)phenyl)ethoxy]-3-(4-fluorophenyl)-tetrahydropyran-4- carbaldehyde (Description 11; 232mg, 0.5mmol) were suspended in 1,2-dichloroethane (10ml) and stirred at room temperature for 30 minutes. Sodium triacetoxyborohydride (105mg, 0.5mmol) was added and the reaction stirred overnight at room temperature. The mixture was partitioned between dichloromethane and brine. The organic layer was dried (MgSO ) and concentrated in vacuo. The residue was purified by preparative thin layer chromatography, eluting with 7.5% methanol/dichloromethane, to give the title compound (138mg, 70%). m/z (ES+) 786 (M+H)⁺.

DESCRIPTION 13 (lS,4i?f)-4,7₉7-Trimethyl-3-oso-2-o-fa-bicyclo[2.2.1]heptane-l-carbosylic cid

(4«)-8-{(2«₅3i?_J4«)-2-[(li?)-l-(3_sS-bi§(trifluørometlιyl)phenyl)ethojiy]-3-(4- fluorophenyl)-tetrahydropyran-4-ylmethyI}-2-oxa-8-aga-spiro[4.5]dec-4-yI ester Solid sodium triacetoxyborohydride (107mg, 0.504mmol) was added to a stirred mixture of 2-[l-{ (2R,3R,4R)-2-[(lR)-l-(3,5-bis(trifluoromethyl)phenyl)ethoxy]-3-(4- fluorophenyl)-tetrahydropyran-4-carbaldehyde (Description 11; 121mg, 0.36mmol), (lS,4R)-4,7,7-trimethyl-3-oxo-2-oxa-bicyclo[2.2.1]heptane-l-carboxylic acid (4R)-2- oxa-8-aza-spiro[4.5]dec-4-yl ester trifluoroacetate (Description 10; 130mg), triethylamine (0.1ml, 0.72mmol) and dichloromethane (3ml) at room temperature. The mixture was stirred for 45 minutes, quenched with saturated aqueous NaHCO₃ and extracted into dichloromethane. The organic extract was dried (Na₂SO ) and concentrated. The residue was purified by preparative TLC (dichloromethane:methanol) to give the title compound (lOOmg). The HCI salt was prepared and recrystallised from ethyl acetate:methanol.

1H NMR (400 MHz, CDC1₃): δ 7.67 (IH, s), 7.17 (2H, s), 7.02-6.89 (4H, m), 4.98 (IH, m), 4.95 (IH, q, / 6.3Hz), 4.23-4.08 (3H, m), 3.68 (IH, d, / 8.6Hz), 3.66 (IH, dd, / 1.6, 9.4Hz), 3.58-3.45 (2H, m), 2.50-2.33 (3H, m), 2.25-1.83 (8H, m), 1.81-1.65 (2H, m), 1.57-1.40 (3H, m), 1.36 (3H, d, /6.6Hz), 1.11 (3H, s), 1.02 (3H, s), 0.94 (3H, s); m/z (ES+) 786 (M+H)⁺.

DESCRIPTION 14 (lιS,4i?)-4,7,7-Trimethyl-3-oxo-2-oxa-bicyclo[2.2.1]heptane-l-carboκylic acid

(4_JR)-8-{(2^,3«_s4«)-2-[(lfi)-l-(3,5-bis(trifluoromethyl)phenyI)eflιoxy]-3-(3- bromo-4-fluorophenyϊ)-tetrahydropyran-4-ylmethyl}-2-øxa-8-asa-spϊro[4.5]dec- 4-yl ester (i) (2R,3R,4R)-2-[(lR)-l-(3,5-Bis(trifluoromethyl)phenyl)ethoxy]-3-(3-bromo-4- fluorophenyl)tetrahydropyran-4-carbaldehyde a) (3-Bromo-4-fluorophenyl)acetic acid 3-bromopropyl ester (3-Bromo-4-fluorophenyl)acetyl chloride (30.2g, 0.12mol) was dissolved in dichloromethane (70mL) and the solution was cooled to 0°C. 3-Bromopropanol (24.7g, 0.177mol) in dichloromethane (30mL) was added dropwise and the mixture was stirred at room temperature overnight. The mixture was washed with water, brine, dried (MgSO₄) and the solvent was removed in vacuo. The resulting oil was purified on silica using 5-10% ethyl acetate in hexane to give the product (36g, 82%). 1H NMR (400 MHz, CDC1₃): δ 7.48 (IH, dd, / 2.3, 6.5Hz), 7.19 (IH, ddd, / 2.3, 4.6, 8.5Hz), 7.10 (IH, t, / 8.4Hz), 4.25 (2H, t, /6.1Hz), 3.58 (2H, s), 3.41 (2H, t, /6.6Hz), 2.17 (2H, quintet, /6.2Hz).

b) (3-Bromo-4-fluorophenyl)-3,4,5,6-tetrahydrohydropyran-2-one

Sodium hydride (4.62g, O.llmol) was suspended in dimethylformamide (200mL) and the suspension was warmed to 60°C. To this was added dropwise (3-bromo-4- fluoro)phenylacetic acid 3-bromopropyl ester (from step a) above; 35.3g) in dimethylformamide (20mL) and the mixture was stirred for 30min until all gas evolution ceased. The mixture was cooled and quenched with water (lOmL) cautiously, and then diluted with water (1500mL) and extracted with ethyl acetate (3xl00mL). The pooled organic extracts were washed with brine, dried (MgSO ) and evaporated. The crude residue was purified by chromatography on silica using 10- 30% ethyl acetate in hexane as eluant to give the lactone (17.1g, 63%). 1H NMR (400 MHz, CDC1₃): δ 7.44 (IH, dd, / 2.3, 6.2Hz), 7.17 (IH, ddd, / 2.2, 4.6, 8.5Hz), 7.07 (IH, t, / 8.4Hz), 4.45 (2H, m), 3.72 (IH, dd, / 6.8, 10.2Hz), 2.30 (IH, m), 2.03 (3H, m).

c) (±) 3-Bromo-(3-bromo-4-fluorophenyl)-3_s4,5,6-tetrahydrohydrøpyran-2-one (3-Bromo-4-fluorophenyl)-3,4,5,6-tetrahydrohydropyran-2-one (from step b) above; 14.82g, 0.054mol) was dissolved in carbon tetrachloride (200mL) and benzoyl peroxide (lOOmg) and N-bromosuccinimide (11.63g, 0.065mol) were added. The mixture was heated cautiously to reflux and maintained at reflux for 3h. The mixture was cooled, filtered and concentrated. The crude residue was purified by chromatography on silica using 10-100% ethyl acetate in hexane as eluant to give the bromide (17.9g, 95%).

1H NMR (400 MHz, CDC1₃): δ 7.84 (IH, dd, 72.6, 6.3Hz)₃ 7.57 (IH, ddd, 72.6, 4.4, 8.8Hz), 7.13 (IH, t, 7 8.6Hz), 4.62 (IH, m), 4.42 (IH, m), 2.80-2.60 (2H, m), 2.38 (IH, m), 2.30 (IH, m), 1.88 (IH, m).

d) 3-(3-Bromo-4-fluorophβnyI)-5,6-dmydropyran-2-one

(±) 3-Bromo-(3-bromo-4-fluorophenyl)-3,4,5,6-tetrahydrohydropyran-2-one (from step c) above; 17.9g, 0.05 Imol) was dissolved in tetrahydrofuran (200mL) and lithium bromide (6.6g, 0.076mol) and lithium carbonate (5.6g, 0.076mol) were added. The mixture was heated at reflux for 3h, then cooled. The mixture was filtered to remove inorganic material. The solvent was removed in vacuo and the crude residue was purified by chromatography on silica using 10-50% ethyl acetate in hexane as eluant to give the enone (11.36g, 82%).

1H NMR (400 MHz, CDC1₃): δ 7.68 (IH, dd, 72.8, 6.6Hz), 7.41 (IH, ddd, / 2.2, 4.6, 8.6Hz), 7.11 (IH, t, 78.4Hz), 7.00 (IH, t, 74.6Hz), 4.49 (2H, t, 76.3Hz), 2.64 (2H, dt, /4.6, 6.2Hz).

e) (±) 3-(3-Bromo-4-fluorophenyl)-4-vinyl-3,4,5,6-tetrahydrohydropyran-2-one

Copper iodide (375mg, 5 mol%) was suspended in tetrahydrofuran (300mL) and the mixture was cooled to -78°C. To this mixture was added vinylmagnesium chloride (25mL, 1.7M) followed by dropwise addition of 3-(3-bromo-4-fluorophenyl)-5,6- dihydropyran-2-one (from step d) above; 10.64g, 0.039mol) and trimethylsilyl chloride (4.97mL, 0.039mol). The reaction progress was monitored by thin layer chromatography. The reaction mixture was quenched by pouring, via double-ended needle, onto ammonium chloride (saturated aqueous solution). The mixture was concentrated in vacuo to remove tetrahydrofuran and the residue was extracted with ethyl acetate (3x200mL). The pooled organic extracts were washed with brine, dried (MgSO₄) and evaporated. The crude residue re-dissolved in tetrahydrofuran and 1,8- diazabicyclo[5.4.0]undec-7-ene (0.3mL) was added. This solution was stirred for 4h. Tetrahydrofuran was removed in vacuo and the residue was purified by chromatography on silica using 30-50% ethyl acetate in hexane as eluant to give the vinyl lactone (11.5g, 80%).

1H NMR (400 MHz, CDC1₃): δ 7.37 (IH, d, / 6.6Hz), 7.09 (2H, d, / 7.5Hz), 5.61 (IH, d, 7 10.3Hz)₃ 4.92 (IH, d, 17.1Hz), 4.57- 4.43 (2H, m), 3.44 (IH, d, 7 10.9Hz), 2.78 (IH, m), 2.16 (IH, m), 1.96 (IH, m).

f) (±) 3-(3-Bromo-4-fϊuørophenyl)-4-vinyltetrahydropyran-2-øl

(±) 3-(3-Bromo-4-fluorophenyl)-4-vinyl-3,4,5,6-tetral ydrohydropyran-2-one (from step e) above; 9.5g, 0.032mol) was dissolved in ethanol (90mL) and cerium (IE) chloride was added. The resulting solution was cooled to -30°C and sodium borohydride (1.2g, 0.032mol) was added portionwise. The mixture was stirred for 30min and was then quenched with acetone (18mL). The solvent was removed in vacuo and the residue was dispersed between ethyl acetate and water. The mixture was filtered through Celite™ to remove inorganic material. The combined organic extracts were washed with brine and dried (MgSO₄). The solvent was removed in vacuo to give the lactol as a yellow solid (9.07g, 94%).

1H NMR (400 MHz, CDC1₃) 3:2 mixture: δ 7.48 (0.4H, m), 7.37 (0.6H, m), 7.20-7.16 (0.4H, m), 7.12-7.00 (1.6H, m), 5.51-5.40 (IH, m), 5.16 (0.4H, s), 4.96-4.82 (2H, m), 4.73 (0.6H, dd, 7 2.6, 8.1Hz), 4.22-4.10 (IH, m), 3.75-3.67 (IH, m), 2.97 (0.4H, m), 2.84 (0.6H, m), 2.71 (0.4H, dd, / 2.8, 11.8Hz), 2.54-2.45 (0.6H, m), 2.40-2.32 (IH, m), 1.80-1.60 (2H, m).

g) (2R,3R,45)-2-[(lR)-l-(3,5-Bis(trifluoromethyl)phenyl)ethoxy]-3-(3-bromo-4- fluorophenyl)-4-vinyltetrahydropyran

(±) 3-(3-Bromo-4-fluorophenyl)-4-vinyltetrahydropyran-2-ol (from step f) above; 10.32g, 0.034mol), (lR)-3,5-bis(trifluoromethyl)phenylethanol (llg, 0.042mol), Amberlyst™ (5g) and molecular sieves (8g) were suspended in dichloromethane (20mL) and the mixture was shaken at 40°C for 3 days. The resulting yellow solution was filtered, concentrated in vacuo and was purified by chromatography on silica using 1-5% ethyl acetate in hexane as eluant to give a mixture of isomers 1-3 (which were retained) and the desired isomer 4 (3.6g, 20%) as a colourless oil. 1H NMR (400 MHz, CDC1₃): δ 7.69 (IH, s), 7.26-7.21 (3H, m), 6.98 (IH, t / 8.4Hz), 6.91 (IH, m), 5.43 (IH, ddd, / 7.1, 10.5, 17.4Hz), 4.95 (IH, q, / 6.6Hz), 4.83 (IH, d, / 10.6Hz), 4.80 (IH, d, 7 17.4Hz), 4.16 (IH, d, 7 8.3Hz), 4.14 (IH, m), 3.56 (IH, m), 2.49 (IH, dd, 78.3, 11.6Hz), 2.38 (IH, m), L75-1.60 (2H, m), 1.40 (3H, d, 76.6Hz).

h) (2i?,3«,4^)-2-[(lK)-l-(3,5-Bi§(trϊfluoromethyl)phenyl)ethøxy]-3-(3-bromo-4- luorøphenyl)tetrahydropyran-4-carbaldehyde

(2R,3R,45)-2-[(lR)-l-(3,5-Bis(trifluoromethyl)phenyl)ethoxy]-3-(3-bromo-4-fluoro- phenyl)-4-vinyltetralιydropyran (from step g); 0.5g, 0.924mol) was dissolved in dichloromethane (lOmL) and methanol (7mL) and ozone was bubbled through the solution at -78 °C until a blue coloration persisted. The solution was purged with nitrogen and dimethyl sulfide (0.6mL) was added dropwise. The mixture was allowed to stir overnight. The solvent was removed in vacuo and the residue was partitioned between ethyl acetate and water. The combined organic phase was dried (MgSO₄) and the solvent was evaporated. The residue was purified by chromatography on silica using 10-20% ethyl acetate in hexane as eluant to afford the product aldehyde as a colourless oil (170mg, 34%).

1HNMR (CDCI₃): δ 1.42 (3H, d, 76.6Hz), 1.78-1.89 (2H, m), 2.68-2.74 (IH, m), 3.01 (IH, dd, 77.1, 10.3Hz), 3.59-3.64 (IH, m), 4.10-4.19 (IH, m), 4.28 (IH, d, 77.1Hz), 4.97 (1 H, q, / 6.6Hz), 7.03 (IH, t, / 8.3Hz), 7.08-7.11 (IH, m), 7.31 (2H, s), 7.39 (IH, dd, /2.2, 6.6Hz), 7.72 (IH, s), 9.51 (IH, d, / 1.5Hz).

(ii) (15,4R)-4,7,7-Trimethyl-3-oxo-2-oxa-bicyclo[2.2.1]heptane-l-carboxylic acid (4R)-8-{(2R,3R,4R)-2-[(lR)-l-(3,5-bis(trifluoromethyl)phenyl)ethoxy]-3-(3- bromo-4-fluorophenyl)-tetrahydropyran-4-ylmethyl}-2-oxa-8-aza-spiro[4.5]dec- 4-yl ester

(lS,4R)-4,7,7-Trimethyl-3-oxo-2-oxa-bicyclo[2.2. l]heptane-l-carboxylic acid (4R)-8- aza-8-benzyloxycarbonyl-2-oxa-spiro[4.5]dec-4-yl ester (Description 9, isomer B; lOOmg, 0.21mmol) was dissolved in methanol (20ml) and trifluoroacetic acid (0.1ml) and Pd (10% on carbon, 20mg) were added under nitrogen. The mixture was purged with hydrogen gas and stirred for 30 min under a balloon of hydrogen. The mixture was purged with nitrogen and filtered to remove catalyst; solvents were removed in vacuo to give the amine as a colourless oil. m/z 338 ( ⁺ + 1, 100%).

The amine salt was dissolved in dichloroethane (3ml) and triethylamine (0.056ml, 0.4mmol) followed by (2R,3R,4R)-2-[(lR)-l-(3,5-bis(trifluoromethyl)phenyl)ethoxy]- 3-(3-bromo-4-fluorophenyl)tetral ydropyran-4-carbaldehyde (Description 14(i); 130mg, 0.25mmol) and sodium triacetoxyborohydride (63mg, 0.318mmol) and the resulting mixture was stirred for 30min. The mixture was then diluted with dichloromethane and washed with sodium bicarbonate (saturated aqueous solution). The organic extracts were pooled, washed with brine, dried (MgSO₄) and concentrated in vacuo. The residue was purified by chromatography on silica gel using 1-5% methanol in dichloromethane as eluant. This afforded the product as a white solid (150mg, 83%).

This was further purified by recrystallisation of the HCI salt from ether-ethyl acetate to give the product as white crystals (140mg).

1H NMR (500MHz, CDC1₃): δ 7.69 (IH, s), 7.22 (IH, dd overlapped, / 2.0Hz), 7.21 (3H, s), 6.98 (IH, t, / 8.3Hz), 6.92-6.89 (IH, m), 5.00 (IH, dd, / 1.3, 4.5Hz), 4.95 (IH, q, / 6.6Hz), 4.18 (IH, dd, / 11.5, 4.7Hz), 4.17-4.13 (IH, m), 4.10 (IH, d, / 8.3Hz), 3.72 (IH, d, / 8.3Hz), 3.66 (IH, d, / 11.0Hz), 3.54 (IH, d, / 8.5Hz), 3.50 (IH, br d), 2.43-2.33 (3H, m), 2.17-2.11 (IH, m), 2.07-1.83 (9H, m), 1.72-1.62 (2H, m), 1.50-1.38 (3H, m), 1.38 (3H, d, / 6.6Hz), 1.11 (3H, s), 1.02 (3H, s), 0.94 (3H, s); m/z (ES+) 864 (M⁺+1, 100%), 866 (M⁺+1, 95%).

DESCRIPTION 15

[(2R,3R,4R)-2-[(lR)-l-(3,5-Bis(trifluoromethyl)phenyl)ethoxy]-3-(3,4- difluorophenyl)-tetrahydropyran-4-yl]methanol

Sodium borohydride (24mg, 0.63mmol) was added to solution of 2-[l-{(2R,3R,4R)-2- [(lR)-l-(3,5-bis(trifluoromethyl)phenyl)ethoxy]-3-(3,4-difluorophenyl)- tetrahydropyran-4-carbaldehyde (see Example 2 in WO 02/16344; 107mg, 0.23mmol) in methanol (3ml). The mixture was stirred for 30 minutes then quenched with saturated aqueous NaHCO₃ and extracted into ethyl acetate. The combined organic extracts were dried and concentrated to give the title compound (104mg). 1H NMR (360 MHz, CDC1₃): δ 7.70 (IH, s), 7.21 (2H, s), 7.05 (IH, m), 6.97 (IH, ddd, / 1.8, 7.4, 10.9Hz), 6.79 (IH, m), 4.97 (IH, q, 76.7Hz), 4.22-4.11 (2H, m), 3.55 (IH, dt, 72.5, 12.3Hz), 3.39 (IH, m), 3.24 (IH, m), 2.57 (IH, dd, 78.4, 11.2Hz), 1.94- 1.78 (2H, m), 1.68 (IH, m), 1.39 (3H₃ d, 76.7Hz), 1.18 (IH, m).

DESCRIPTION 16 Methane§ulfonk acid [(2^,3^₅4«)-2-[(li?)-l-(3,5-Bis(trifluoromethyl)- phenyl)ethosy]-3-(3,4-difluorophenyl)-fetrahydropyran-4-yl]methyl ester Methanesulfonyl chloride (50μl, 0.63mmol) was added to a mixture of [(2R,3R,4R)-2- [(lR)-l-(3,5-bis(trifluoromethyl)phenyl)ethoxy]-3-(3,4-difluorophenyl)- tetrahydropyran-4-yl]methanol (Description 15; 235mg, 0.49mmol), triethylamine (133ml, 0.97mmol), 4-N,N-dimethylaminopyridine (3mg, 0.02mmol) and dichloromethane (5ml) at 0°C. The mixture was stirred for 30 minutes, then quenched with saturated aqueous NaHCO₃ and extracted into dichloromethane. The combined organic extracts were dried and concentrated. The product was used in the next step without further purification.

1H NMR (400 MHz, CDC1₃): δ 7.70 (IH, s), 7.20 (2H, s), 7.09 (IH, dt, 78.6, 9.8Hz), 6.88 (IH, ddd, 72.0, 7.4, ll.OHz), 6.81 (IH, m), 4.97 (IH, q, 76.7Hz), 4.21-4.12 (2H, m), 3.95 (IH, dd, 73.1, 9.8Hz), 3.80 (IH, dd, 77.4, 10.2Hz), 3.55 (2H, dt, 72.4, 12.1Hz), 2.90 (3H, s), 2.58 (IH, dd, / 8.2, 11.7Hz), 2.12 (IH, m), 1.86 (IH, m), 1.78- 1.66 (IH, m), 1.39 (3H, d, /6.7Hz).

DESCRIPTION 17 l-Oxa-8-azaspiro[4.5]decane Hydrochloride

(i) l,l-Dimethylethyl 4-hydroxy-4-(3-trimethylsilyloxypropynyl)-l- piperidinecarboxylate

Trimethyl(2-propynyloxy)silane (11.54ml, 9.62g, 75mmol) was added dropwise to a stirred, cooled (-5°C) solution of ethyl magnesium bromide (1M in tetrahydrofuran, 75ml, 75mmol) in tetrahydrofuran (150ml), maintaining the internal temperature below 0°C. The mixture was stirred at 0°C for 30 minutes, then at room temperature for 90 minutes. The mixture was cooled to -5°C and 1,1-dimethylethyl 4-oxo-l- piperidinecarboxylate (15.0g, 75mmol) was added slowly, maintaining the internal temperature below 0°C. The mixture was stirred at 0°C for 3 hours then at room temperature for 96 hours. Saturated aqueous ammonium chloride (300ml), water (100ml) and ethyl acetate (300ml) were added and the layers were separated. The aqueous layer was extracted with ethyl acetate (200ml) and the combined organic fractions were dried (MgSO₄) and the solvent was evaporated under reduced pressure to give the title compound (24.4g, 100%).

1H NMR (400 MHz, CDC1₃): δ 4.36 (2H, s), 3.80-3.70 (2H, m), 3.35-3.25 (2H, m), 1.95-1.85 (2H, m), 1.78-1.60 (3H, m), 1.48 (9H, s), 0.2 (9H, s).

(ii) l,l-Dimethylethyl 4-hydroxy-4-(3-hydroxypropynyl)-l-piperidinecarboxylate

Tetrabutylammonium fluoride (IM in tetrahydrofuran, 80ml, 80mmol) was added to a solution of 1,1-dimethylethyl 4-hydroxy-4-(3-trimethylsilyloxypropynyl)-l- piperidinecarboxylate (from step (i) above; 24.4g, 75mmol) in tetrahydrofuran (300ml) and the mixture was stirred at room temperature for 18 hours. The solvent was evaporated under reduced pressure and water (200ml) was added. The mixture was extracted with ethyl acetate (2 x 200ml) and the combined organic fractions were washed with water (2 x 200ml) and brine (200ml), dried (MgSO ) and the solvent was evaporated under reduced pressure to give the title compound as an orange oil (16.8g, 88%).

1H NMR (400 MHz, CDC1₃): δ 4.32 (2H, s), 3.80-3.68 (2H, m), 3.32-3.22 (2H, m), 1.93-1.83 (2H, m), 1.75-1.65 (2H, m), 1.62 (IH, br s), 1.46 (9H, s).

(iii) l,l-Dimethylethyl 4-hydroxy-4-(3-hydroxypropyl)-l-piperidinecarboxylate

Palladium on carbon (5%, 800 mg) was added to a solution of 1,1-dimethylethyl 4- hydroxy-4-(3-hydroxypropynyl)-l -piperidinecarboxylate (from step (ii) above; 8.37g, 32.8mmol) in ethanol (400ml), acetic acid (40ml) and water (5ml) and the mixture was shaken under an atmosphere of hydrogen (40 psi) for 20 hours. The mixture was filtered through Hyflo™ and the solvent was evaporated under reduced pressure. The residue was purified by flash column chromatography on silica gel, eluting with isohexane/EtOAc (50:50 increasing lo 0:100), to give the title compound (4.84g, 57%). 1H NMR (400 MHz, CDC1₃): δ 3.85-3.75 (2H, m), 3.70 (2H, t, /6Hz), 3.18 (2H, br t, / 14Hz), 2.00 (2H, br s), 1.73-1.65 (2H, m), 1.63-1.48 (6H, m), 1.46 (9H, s).

(iv) Ijl-Dimethylethyl l-oxa-8-aza§piro[4.5]dβcanβ-8-carbosylate

A solution of diethyl azodicarboxylate (3.35ml, 21.3mmol) in tetrahydrofuran (50ml) was added over 15 minutes to a stirred, cooled (0°C) solution of 1,1-dimethylethyl 4- hydroxy-4-(3-hydroxypropyl)-l-piperidinecarboxylate (from step (iii) above; 4.6g, 17.8mmol) and triphenylphosphine (5.58g, 21.3mmol) in tetrahydrofuran (150ml) and the mixture was stirred at 0°C for 1 hour, then at room temperature for 24 hours. The solvent was evaporated under reduced pressure and the residue was purified by flash column chromatography on silica gel, eluting with isohexane EtOAc (75:25 increasing to 50:50), to give the title compound (3.18g, 70%). 1H NMR (400 MHz, CDC1₃): δ 3.83 (2H, t, 77Hz), 3.63-3.53 (2H, m), 3.38-3.28 (2H, m), 1.93 (2H, quin., 77Hz), 1.69 (2H, t, 77Hz), 1.63-1.48 (4H, m), 1.46 (9H, s); m/z (ES⁺) 242 (M+l).

(v) l-Oxa-8-azaspiro[4.5]decane Hydrochloride

Methanolic hydrogen chloride (3M, 20ml) was added over 10 minutes to a stirred, cooled (0°C) solution of 1,1-dimethylethyl l-oxa-8-azaspiro[4.5]decane-8-carboxylate (from step (iv) above, 3.18g, 13.2mmol) in methanol (10ml) and the mixture was stirred at room temperature for 3 hours. The solvent was evaporated under reduced pressure to give the title compound (2.29g, 98%).

1H NMR (400 MHz, CDC1₃): δ 3.85 (2H, t, / 7Hz), 3.27-3.15 (4H, m), 1.98 (2H, quin., 7Hz), 1.86-1.75 (6H, m); m/z (ES⁺) 142 (M+l).

DESCRIPTION 18

2,2-Dinιβthyl-l-oxa-8-agaspiro[4.5]dβeanβ

(i) l,l-Dimethylethyl 4-(3-ethoxy-3-oxo-l-propynyl)-4-hydrosy-l- piperidinecarboxylate rc-Butyllithium (175ml, 0.28mol) was added dropwise over 45 minutes to a stirred, cooled (-70°C) solution of ethyl propiolate (32ml, 0.32mol) in tetrahydrofuran (250ml). The mixture was stirred at -70°C for 10 minutes, then a solution of 1,1- dimethylethyl 4-oxo-l-piperidinecarboxylate (18.6g, 0.093 mol) in tetrahydrofuran (250ml) was added dropwise over 1 hour maintaining the internal temperature below -70°C. The mixture was stirred at -70 °C for 1 hour and then acetic acid (21ml) in tetrahydrofuran (50ml) was added. The mixture was warmed to room temperature and the solvent was evaporated under reduced pressure. Saturated aqueous sodium hydrogen carbonate (300ml) and ethyl acetate (650ml) were added and the layers were separated. The aqueous fraction was extracted with ethyl acetate (22i 650ml) and the combined organic fractions were washed with brine, dried (Na₂SO₄) and the solvent was evaporated under reduced pressure. The residue was purified by chromatography on a short silica gel column, eluting with 1:1 isohexane:ethyl acetate, to give the title compound (29.2g, contains trace impurity).

1H NMR (400 MHz, CDC1₃): δ 4.24 (2H, q, 77Hz), 3.73-3.68 (2H, m), 3.38-3.31 (2H, m), 1.98-1.91 (2H, m), 1.80-1.72 (2H, m), 1.46 (9H, s), 1.31 (3H, t, 7Hz); m/z (ES⁺) 298 (M+l).

(ii) 1,1-Dimethylethyl 4-(3-ethoxy-3-oxoprop-l-yl)-4-hydroxy-l- piperidinecarboxylate

Palladium on carbon (5%, 1 g) in water (10ml) was added to a solution of

1 , 1 -dimethylethyl 4-(3 -ethoxy-3-oxo- 1 -propynyl)-4-hydroxy- 1 -piperidinecarboxylate

(from step (i) above; 14.6g, 0.046 mol) in ethanol (200ml) and the mixture was shaken under hydrogen (45 psi) for 90 minutes. The mixture was filtered through a glass fibre pad and the solvent was evaporated under reduced pressure to give the title compound.

1H NMR (400MHz, CDC1₃) δ 4.14 (2H, q, / 7Hz), 3.80 (2H, br s), 3.20-3.11 (2H, m),

2.45 (2H, t, 77Hz), 1.81 (2H, t, 77Hz), 1.58-1.42 (4H, m), 1.45 (9H, s), 1.26 (3H, t, /

7Hz); m/z (ES⁺) 302 (M+l).

(iii) 1,1-Dimethylethyl l-oxa-2-oxo-8-aEaspiro[4.5]dβcane-8-carfooxylatβ ?-Toluenesulfonic acid (1.75g, 9.2mmol) was added to a solution of 1,1-dimethylethyl 4-(3-ethoxy-3-oxoprop-l-yl)-4-hydroxy-l-piperidinecarboxylate (from step (ii) above; 27.63g, 0.092mol) in toluene (250ml) and the mixture was heated under reflux for 3 hours. The mixture was cooled and the solvent was evaporated under reduced pressure. Water (400ml) and ethyl acetate (400ml) were added and the layers were separated. The aqueous fraction was extracted with ethyl acetate (200ml). The combined organic fractions were washed with brine, dried (MgS0₄) and the solvent was evaporated under reduced pressure to give the title compound (20.86g, 88%). 1H NMR (400 MHz, CDC1₃): δ 3.84-3.79 (2H, m), 3.26 (2H, br t), 2.62 (2H, t, / 7Hz), 2.05 (2H, t, 77Hz), 1.83-1.79 (2H, m), 1.68-1.62 (2H, m), 1.46 (9H, s); m/z (ES⁺) 256 (M+l).

(iv) l,l-Dimethylethyl 4-hydroxy-4-(3-hydroxy-3-methylbut-l-yl)-l- piperidinecarboxylate

1,1-Dirnethylethyl l-oxa-2-oxo-8-azaspiro[4.5]decane-8-carboxylate (from step (iii) above; 5.0g, 19.58mmol) in tetrahydrofuran (150ml) was added dropwise over 45 min. to a stirred, cooled (0 °C) solution of methyl magnesium chloride (3M in tetrahydrofuran, 19.58ml, 58.75mmol) in tetrahydrofuran (150ml). The mixture was allowed to warm to room temperature and stirred overnight. The mixture was poured into saturated aqueous ammonium chloride (200ml) and extracted with ethyl acetate (200ml). The organic fraction was washed with brine, dried (Na₂SO₄) and the solvent was evaporated under reduced pressure. The residue was purified by flash column chromatography on silica gel, eluting with ethyl acetate, to give the title compound (3.9g, 69%).

1H NMR (400 MHz, CDCI3): δ 3.85-3.75 (2H, m), 3.20-3.10 (2H, m), 2.55-2.25 (2H, m), 1.58-1.45 (6H, m), 1.46 (9H, s), 1.24 (6H, s); m z (ES⁺) 214 (M+l-QHs-HjO).

(v) 2,2-Dimethyl-l-oxa-8-azaspiro[4.5]decane

Trifluoroacetic acid (10ml) was added to a solution of 1,1-dimethylethyl 4-hydroxy-4- (3-hydroxy-3-methylbut-l-yl)-l-piperidinecarboxylate (from step (iv) above; 3.9g, 13.57mmol) in dichloromethane (10ml) and the mixture was stirred at room temperature overnight. The solvent was evaporated under reduced pressure and the residue was purified by flash column chromatography on silica gel, eluting with CH₂Cl₂/MeOH/NH₃(Aq.) (90:10:1) to give the title compound (2.04g, 89%). 1H NMR (400MHz, CDC1₃): δ 3.27-3.17 (4H, m), 1.95-1.82 (6H, m), 1.70 (2H, d, / 13.4Hz), 1.22 (6H, s); m/z (ES⁺) 170 (M+l).

DESCRIPTION 19 6- xa-2-azaspiro[3.4]øefan-S-©l

(i) l-Bemayloxycarfoonyl-S-asetidinecarboxylk acid

3-Azetidinecarboxylic acid (4.0g, 39.6 mmol) was dissolved in IN sodium hydroxide solution (40mL) and cooled to 0°C. Benzyl chloroformate (5.9mL, 41mmol) was added followed by further IN sodium hydroxide solution (41mL) dropwise. The mixture was stirred vigorously for 16hrs then made acidic with 2N hydrochloric acid.

This suspension was extracted with dichloromethane (2xl00mL) and the extracts dried over MgSO₄. Concentration yielded the title compound (9.3g, 39.6mmol,

100%).

1H NMR (400MHz, CDC1₃): δ 7.37-7.29 (5H, m), 5.10 (2H, s), 4.21 (4H, d, / 7.5Hz),

3.43 (IH, quintet, /7.5Hz).

(ii) Methyl l-benzyloxycarbonyl-3-azetidinecarboxylate l-Benzyloxycarbonyl-3-azetidinecarboxylic acid (from step (i); 9.3g, 39.6mmol) was dissolved in methanol (lOOmL) and toluene (lOOmL), and cooled to 0°C. A solution of 2M trimethylsilyldiazomethane in hexanes was then added dropwise until bubbling had ceased and the yellow colour persisted. Acetic acid was then added dropwise until the yellow colour disappeared. Concentration of the solution yielded the title compound as an oil (9.48g, 38mmol, 96%).

1H NMR (400MHz, CDC1₃): δ 7.36-7.31 (5H, m), 5.10 (2H, s), 4.19 (4H, d, / 7.8Hz), 3.75 (3H, s), 3.39 (IH, quintet, 77.8Hz).

(iii) Bensyl 3-(l,2-dihydroxyethyl)-3-(hydroxymethyϊ)asetidine-l-carboxylate Methyl l-benzyloxycarbonyl-3-azetidinecarboxylate (from step (ii); lO.lg, 40mmol) was dissolved in tetrahydrofuran (120mL) and cooled to -78°C. A solution of lithium hexamethyldisilazide in tetrahydrofuran (IM, 58mL, 58mmol) was slowly added and the solution warmed to 0°C. After 15 mins. at this temperature, the resulting solution was cooled back to -78°C and tert-butyldimethylsilyloxy acetaldehyde was added and stirred to room temperature over 2hrs and quenched with water (200mL). This suspension was extracted with ethyl acetate (2x200mL). The extracts were dried (MgSO₄) and concentrated. The residue was flushed through silica with 25% ethyl acetate in hexanes to give a 1:2 mixture of 1-benzyl 3-methyl 3-{2-{[tert- butyl(dimethyl)silyljoxy }- 1 -hydroxyethyl)azetidine- 1 ,3-dicarboxylate : starting material (4.24g).

This mixture was dissolved in tetrahydrofuran (30mL) and treated with lithium borohydride (395mg, 18mmol). After 2hrs at room temperature, the mixture was quenched with IN sodium hydroxide solution (lOOmL) and extracted with ethyl acetate (2xl00mL). The extracts were dried (MgSO₄) and concentrated to give crude benzyl 3-(2-{[tgrt-butyl(dimethyl)silyl]oxy}-l-hydroxyethyl)-3-(hydroxylmethyl) azetidine-1-carboxylate. This was dissolved in tetrahydrofuran (lOOmL) and treated with IM tetra-n-butylammonium fluoride in tetrahydrofuran (lOmL, lOmmol). After stirring for lhr at room temperature, water (200mL) was added and the mixture extracted with ethyl acetate (2x200mL). The combined extracts were dried (MgSO₄) and concentrated. The residue was purified by silica chromatography to give the title compound as an oil (956mg, 3.4mmol).

1H NMR (400 MHz, CDC1₃): δ 7.37-7.29 (5H, m), 5.09 (2H, s), 3.96-3.64 (9H, m), 3.13 (2H, br m), 1.69 (IH, br m).

(iv) Benzyl 8-hydroxy-6-oxa-2-azaspiro[3.4]octane-2-carboxyIate

Benzyl 3-(l,2-dihydroxyethyl)-3-(hydroxymethyl)azetidine-l-carboxylate (from step (iii); 956mg, 3.4mmol) was dissolved in dichloromethane (150mL) and dibutyltin oxide (17mg) was added. p-Toluenesulfonyl chloride (686mg, 3.6mmol) was added and the solution was cooled to 0°C. Triethylamine (520μL, 3.75mmol) was added dropwise and the solution was then allowed to stir at ambient temperature for 16hrs. Further triethylamine (260μL, 1.9mmol) was added and after stirring for 2hrs, water (250mL) was added. The mixture was extracted with dichloromethane (2x250mL), and the extracts dried (MgSO ) then concentrated. The residue was purified by silica chromatography to give the title compound as an oil (456mg, 1.7mmol, 51%). 1H NMR (400 MHz, CDC1₃): δ 7.37-7.29 (5H, m), 5.10 (2H, s), 4.31-4.27 (2H, m), 3.97 (2H, dd, 79.1, 24Hz), 3.94-3.91 (IH, m), 3.86 (IH, d, 79.5Hz), 3.85 (2H, dd, 7 9.1, 20Hz), 3.74 (IH, dd, / 1.9, 10.2Hz), 1.95 (IH, d, /4.4Hz).

(v) 6-Oxa-2-asaspiro[3.4]øctan-0-ol

Benzyl 8-hydroxy-6-oxa-2-azaspiro[3.4]octane-2-carboxylate (from step (iv); 456mg,

1.7mmol) was dissolved in methanol (20mL) and 10% palladium on carbon (50mg) added. This suspension was hydrogenated at 20psi hydrogen for 2hrs then filtered.

The filtrate was concentrated to give the title compound as a gum (214mg, 1.66mmol,

98% yield). m/z (ES⁺) 130 (M+H).

DESCRIPTION 20 4-Hydroxy-l-oxa-8-azaspiro[4.5]decane

(i) tert-Butyl 4-(3-{[te/ -butyl(dimethyl)silyl]oxy}propylidene)piperidine-l- carboxylate

3-{[tert-Butyl(dimethyl)silyl]oxy}propyl)(triphenyl)phosphonium bromide (J. Med Chem 1990, 33, 1958) (5!5g, O.Olmol) was suspended in toluene and azeotroped to remove any moisture. It was then suspended in 1,2-dimethoxyethane (20mL) and n- butyllithium (7.0mL, 1.6M. 0.01 lmol) was added dropwise until a yellow coloration persisted. tert-Butyl 4-oxopiperidine-l-carboxylate (2g, O.Olmol) was added and the mixture was heated at reflux overnight. The cooled mixture was diluted with water and extracted with ethyl acetate (2 x 50mL), dried (MgSO ) and concentrated in vacuo. The crude yellow oil was purified on silica using 10-30% ethyl acetate in iso- hexane as eluant.

1H NMR (500 MHz, CDCI₃): δ 0.01 (6H, s), 0.84 (9H, s), 2.06-2.18 (4H, m), 2.20 (2H, t, 77.0Hz), 3.33 (4H, multiplet centre (mc)), 3.53 (2H, t, 77.0Hz), 5.24 (IH, t, 7 7.2Hz); m/z (ES⁺) 256 (M⁺+1-100, 100%).

(ii) tø^-Butyl 4-(l,3-dihydroxypropyl)-4-hydroxypiperidine-l-earboxyIate tert-Butyl 4-(3-{[tert-butyl(dimethyl)silyl]oxy}propylidene)piperidine-l-carboxylate (from step (i) above; 0.9g) was dissolved in tetrahydrofuran (lmL); tetra-n-butylammonium fluoride (2.5mL, IM in THF) was added and the mixture was stirred for 30 min until tic analysis (50:50 ethyl acetate:hexane) showed that all starting material had reacted. This mixture was concentrated in vacuo. The crude product was re-dissolved in tert-butanol (5mL) and water (lmL) and N- methylmorpholine N-oxide (322mg) was added and stirred until dissolved. Osmium tetroxide (0.15mL, 2.5% in t-butanol) was added and the mixture was stirred for 12h. This mixture was concentrated in vacuo. The crude product was purified by chromatography on silica using 1-6% methanol in dichloromethane as eluant to afford the product (480mg).

1H NMR (500 MHz, CDC1₃): δ 1.45 (9H, s), 1.40-1.85 (6H, m), 3.02-3.15 (2H, m), 3.60 (IH, dd, 7 12, 4Hz), 3.85-4.00 (4H, m); m/z (ES⁺) 176 (M⁺+l-100, 100%).

(iii) førtf-ButyI 4-hydroxy-l-oxa-8-azaspiro[4.5]decane-8-carboxylate tert-Butyl 4-(l,3-dihydroxypropyl)-4-hydroxypiperidine-l-carboxylate (from step (ii) above; 480mg) was dissolved in dichloromethane and p-toluenesulfonyl chloride was added followed by triethylamine (0.49mL) and the mixture was stirred for 12h. An additional aliquot of triethylamine was added (0.49mL) and the mixture stirred for 48h. This mixture was concentrated in vacuo. The crude product was purified by chromatography on silica using 50-95% ethyl acetate in iso- exane as eluant to afford the product (300mg).

1H NMR (500 MHz, CDC1₃): δ 1.45 (9H, s), 1.48-1.67 (2H, br m), 1.95 (IH, mc), 2.32 (IH, mc), 3.18 (2H, mc), 3.72-3.83 (IH, br m), 3.85 (2H, mc), 3.99 (2H, mc); m/z (ES⁺) 158 (M⁺+1-100, 100%).

(iv) 4-Hydroxy-l-oxa-8-azaspiro[4.5]decane trifluoroacetate tert-Butyl 4-hydroxy-l-oxa-8-azaspiro[4.5]decane-8-carboxylate (from step (iii) above; 300mg) was dissolved in dichloromethane and trifluoroacetic acid (2mL) was added. The solution was stirred for 2h and was concentrated in vacuo to afford the product as a colorless oil. 1H NMR (500 MHz, CDC1₃): δ 1.61-1.66 (IH, m), 1.89-2.06 (3H, m), 2.27-2.31 (2H, m), 3.20-3.33 (3H, m), 3.35-3.42 (IH, m), 3.85 (IH, mc), 3.99 (IH, q, 7 8.5Hz), 4.07 (IH, dd, 76.5, 4.0Hz); m/z (ES⁺) 158 (M⁺+1, 100%).

DESCRIPTION 21 4-Hydroxy-3,3-dimethyl-2-øxa-8-asaspiro[4.5]decane

(i) Bensyl 4-(acetyloxy)-3,3-dinιetlιyl-l-oxo-2-oxa-8-asaspiro[4.S]decane-§- carboxylate

A solution of 1 -benzyl 4-ethyl piperidine-l,4-dicarboxylate (2.35g) was dissolved in tetrahydrofuran (30mL) and was cooled to -78°C. Lithium bis(trimethylsilyl)amide (9mL, IM) was added dropwise under nitrogen and the solution was stirred for 30min at -20°C. The solution was re-cooled to -78°C and l,l-dimethyl-2-oxoethyl acetate (1.17g) in tetrahydrofuran (5mL) was added and the mixture was stirred for 30min. The mixture was quenched (NaHCO₃, 30mL) and the mixture was extracted with ethyl acetate (2 x 50 mL). The extracts were combined, washed with brine, dried (MgSO₄) and were concentrated in vacuo. The crude product was purified by chromatography on silica using 25-75% ethyl acetate in wo-hexane as eluant to afford the product (1.4g). m/z (ES⁺) 376 (_VT+1, 100%).

(ii) Benzyl 4-(l,2-dihydroxy-2-methylpropyl)-4-(hydroxymethyl)piperidine-l- carboxylate

Benzyl 4-(acetyloxy)-3,3-dimethyl-l-oxo-2-oxa-8-azaspiro[4.5]decane-8-carboxylate (from step (i) above; 1.4g) was dissolved in tetrahydrofuran and lithium borohydride (81mg) was added. The mixture was heated under reflux for 2h, cooled and quenched carefully with water and HCI (30mL, 5N). The mixture was extracted with ethyl acetate and dried (MgSO ), and concentrated in vacuo. The crude product was purified by chromatography on silica using 2-5% methanol in dichloromethane as eluant to afford the product (340mg). m/z (ES⁺) 338 (M⁺+1, 80%) 320 (M÷+l-18, 100%).

(iii) Benzyl 4-hydroxy-3,3-dimethyl-2-oxa-8-azaspiro[4.5]decane-8-carboxylate Benzyl 4-(l,2-dihydroxy-2-methylpropyl)-4-(hydroxymethyl)piperidine-l-carboxylate (from step (hi) above; 340mg) was dissolved in dichloromethane and p- toluenesulfonyl chloride (190mg) was added, followed by triethylamine (0.14mL). This mixture was stirred for 2 h and a second aliquot of triethylamine was added and the mixture was stirred for 12h. The mixture was concentrated in vacuo. The crude product was purified by chromatography on silica using 50-100% ethyl acetate in iso- hexane as eluant to afford the product (190mg). 1H NMR (500 MHz, CDC1₃): δ 1.18 (3H, s), 1.29 (3H, s), 1.46-1.59 (2H, m), 1.62-

1.78 (2H, m), 3.00-3.13 (2H, m), 3.55 (IH, d, 79.3Hz), 3.73 (IH, d, / 9.3Hz), 3.84- 3.95 (2H, m), 5.12 (2H, s), 7.29-7.36 (5H, m); m/z (ES⁺) 320 (M⁺+1, 100%).

(iv) 4-Hydroxy-3,3-dimethyl-2-oxa-8-azaspiro[4.5]decane

Benzyl 4-hydroxy-3,3-dimethyl-2-oxa-8-azaspiro[4.5]decane-8-carboxylate (from step (iii) above; 180mg) was dissolved in methanol and palladium on carbon (5%) was added. The mixture was hydrogenated at atmospheric pressure for 30 min. The catalyst was removed by filtration through Hyflo™ and the solvent was evaporated in vacuo to afford the product as a white solid. 1H NMR (500 MHz, CDC1₃): δ 1.13 (3H, s), 1.24 (3H, s), 1.45-1.50 (2H, m), 1.67-

1.79 (2H, m), 2.64-2.71 (2H, m), 2.89-3.00 (2H, m), 3.47 (IH, s), 3.59 (IH, d, / 9.2Hz), 3.72 (IH, d, /9.2Hz); m/z (ES⁺) 186 (M⁺+1, 100%).

DESCRIPTION 22 l-Oxa-8-aza[4.5]decan-3-ol

(i) fer?-Butyl 4-hydroxy-4-{3-[(trimethylsilyl)oxy]prop-l-yn-l-yl}piperidine-l- carboxylate

Ethyl magnesium bromide in tetrahydrofuran (55mL, IM, 55mmol) was slowly added to a solution of trimethyl(prop-2-yn-l-yloxy)silane in tetrahydrofuran (40mL) maintaining a temperature of 0°C. After the complete addition the mixture was stirred at ambient temperature for 45 min before a solution of tert-butyl piperidin-4-one-l- carboxylate (lOg, 50mmol) in tetrahydrofuran (40mL) was slowly added at 0°C. Subsequently the reaction mixture was stirred for 18h at ambient temperature. Then saturated ammonium chloride solution was added and the mixture was stirred vigorously until the dissolution of all solids. The phases were separated and the aqueous was extracted with ethyl acetate. The combined organic phases were washed with brine, dried over magnesium sulfate, filtered and concentrated under reduced pressure to give 16.42g of desired product (96 %) as a yellow oil. 1H NMR (360 MHz, CDC1₃): δ 4.16 (2H, s), 3.55 (2H, br m), 3.12 (2H, ddd, / 3.8, 9.3, 12.8Hz), 1.74-1.66 (2H, m), 1.53 (2H, ddd, 74, 9, 12.8Hz), 1.28 (9H, s).

(ii) feιf-ButyI 4-hydroxy-4-(3-hydrøxyprop-l-yn-l-yl)piperidine-l-carboxylate tert-Butyl 4-hydroxy-4- { 3 - [(trimethylsilyl)oxy]prop- 1-ynl-l-yl} piperidine- 1 - carboxylate (crude from step (i); 16.42g, ca. 50mmol) was stirred with tetrabutylammonium fluoride (90mmol) in tetrahydrofuran (170mL) at ambient temperature for 18h. Then the solvent was removed in vacuo and the residue partitioned between water and ethyl acetate. The organic phase was separated and the aqueous was extracted with ethyl acetate. The combined organic phases were washed with brine, dried over magnesium sulfate, filtered and concentrated under reduced pressure to give the crude product as an amber oil, which was purified by flash chromatography on silica gel, eluting with 5 % methanol in dichloromethane to yield the desired diol (10.87g, 85 %).

1H NMR (360 MHz, CDC1₃): δ 4.32 (2H, br s), 3.80-3.69 (2H, br m), 3.28 (2H, ddd, 7 3.5, 9.2, 13.2Hz), 1.92-1.84 (2H, br m), 1.74 (2H, ddd, 73.8, 8.9, 12.7Hz), 1.46 (9H, s).

(iii) tert-Butyl 4-hydroxy-4-(3-hydroxyprop-l-ene-l-yl}piperidine-l-carboxylate tert-Butyl 4-hydroxy-4-(3-hydroxyprop-l-yn-l-yl)piperidine-l-carboxylate (from step (ii); lOg, 39.2mmol) was stirred at ambient temperature with Lindlar's catalyst (5 % Pd on CaCO₃ poisoned with Pb) (4.15g, 2mmol of Pd) in ethanol under hydrogen (p > 1 atm) for 18h. Then the catalyst was filtered off and the filtrate concentrated under reduced pressure to give 10.2g of the desired cw-olefin as a pale yellow oil. 1H NMR (360 MHz, CDC1₃): δ 5.66-5.56 (2H, m), 4.33 (2H, d, 75.2Hz), 3.77-3.67 (2H, m), 3.30-3.24 (2H, m), 1.73-1.63 (4H, m), 1.46 (9H, s). (iv) tert-Butyl l-oxa-8-azaspiro[4.5]dec-3-ene-8-carboxylate

To a solution of tert-butyl 4-hydroxy-4-(3-hydroxyprop-l-ene-l-yl)piperidine-l- carboxylate (from step (iii); 8.79g, 34.2mmol) and triethylamine (7.6g, 75.2mmol) in tetrahydrofuran (170mL), methanesulfonyl chloride (4.31, 37.6mmol) was slowly added so that the internal temperature stayed between -10 and -5°C. After the complete addition the temperature was allowed to rise to 12°C and was kept at that temperature until all starting diol was turned over (monitored by TLC (silica gel, l:lhexane/ethyl acetate)). Subsequently the reaction mixture was stirred for 2 days to complete the cyclisation before it was concentrated under reduced pressure. The residue was treated with water under ice-cooling, followed by extraction with ethyl acetate. The combined organic layers were washed with brine, dried over magnesium sulfate, filtered and concentrated under reduced pressure to give 9g of crude product as yellow oil. This was purified by flash chromatography on silica gel, eluting with a gradient solvent system of 10-20 % ethyl acetate in hexane to give 5.79g of the desired spirocyclic product (64 %).

1H NMR (360 MHz, CDC1₃): δ 5.88 (IH, ddd, 7 1.7, 1.7, 6.1Hz), 5.74 (IH, ddd, / 2.2, 2.2, 6.3Hz), 4.64 (2H, dd, / 1.6, 2.2Hz), 3.79-3.69 (2H, br m), 3.28 (2H, ddd, / 3.64, 10.5, 13.8Hz), 1.68-1.54 (4H, m), 1.46 (9H, s).

(v) tert-Butyl l-oxa-8-aza[4.5]decan-3-oI-8-carboxylate

Borane-tetrahydrofuran-complex solution in tetrahydrofuran (16.7mL, IM. 16.7mmol) was added to a solution of tert-butyl l-oxa-8-azaspiro[4.5]dec-3-ene-8- carboxylate (from step (iv) above; 2g, 8.37mmol) in tetrahydrofuran (25mL) at 0°C. After lh at room temperature, a mixture of 35 % w/w hydrogen peroxide solution (6mL, ca. 67mmol) and 4 M sodium hydroxide solution (17mL) was slowly added at 0°C. Then the reaction mixture was stirred at room temperature for 18h before it was partitioned between water and ethyl acetate. The aqueous phase was extracted with ethyl acetate. The combined organic phases were washed with saturated sodium sulfite solution, then brine and finally dried over magnesium sulfate, filtered and concentrated to give the crude mixture of products as a colourless oil. The mixture was separated by flash chromatography on silica gel, eluting with a gradient solvent system of 50-100 % ethyl acetate in hexane, then 1-2 % methanol in ethyl acetate to furnish 1.03g of the title compound (48 %). 1H NMR (360 MHz, CDC1₃): δ 4.51-4.49 (IH, m), 3.92 (IH, dd, /4.4, 10.1Hz), 3.84- 3.79 (IH, m), 3.59 (IH, br s), 3.38-3.30 (IH, m) 1.96 (IH, dd, /6.3, 13.6Hz), 1.85- 1.79 (2H, m), 1.73-1.63 (2H, m), 1.58-1.50 (2H, m), 1.45 (9H, s).

(vi) l- xa-8-asa[4.5]decaϊi-3-øl Irifluoroacetate tert-Butyl l-oxa-8-aza[4.5]decan-3-ol-8-carboxylate (from step (v) above; Ig,

3.89mmol) was stirred in a 1:1 v/v mixture of trifluoroacetic acid and dichloromethane (8mL) at ambient temperature for 0.5h. The reaction mixture was concentrated in vacuo to give 1.55g of a pale amber oil.

1H NMR (360 MHz, CDCI₃): δ 4.58-4.61 (IH, m), 3.87-4.02 (2H, m), 3.37-3.47 (2H, m), 2.26-1.75 (6H, m); m/z (ES⁺) 158.

DESCRIPTION 23

(2R,3R,4R,αR5)-α-(3-Buten-l-yl)-2-{(lR)-l-[3,5- bis(trifluoromethyl)phenyl]ethoxy}tetrahydro-3-phenyl-2/7-pyran-4-methanol

3-Butenylmagnesium bromide (0.5M in tetrahydrofuran, 75 mL, 37.5 mmol) was added slowly to a stirred, cooled -78 °C solution of (2R,3R,4R)-2-{(lR)-l-[3,5- bis(trifluoromethyl)phenyl]ethoxy}tetrahydro-3-phenyl-2H-pyran-4-carboxaldehyde (WO 00/56727A1; 11.1 g, 24.9 mmol) in tetrahydrofuran (125 mL). The mixture was stirred at -78 °C for 3 hours, then at room temperature for 1.5 hour. Saturated aqueous ammonium chloride (20 mL) was added slowly, followed by ethyl acetate (300 mL). The layers were separated and the organic layer was washed with brine (100 mL). The combined aqueous layers were extracted with ethyl acetate (100 mL), the combined organic layers were dried (Na₂SO ), and the solvent was evaporated under reduced pressure. The residue was purified by column chromatography on silica gel, eluting with hexane EtOAc (97:3 increasing to 80:20), to give the title compound (8:1 mixture of alcohol diastereoisomers) as a colorless oil (10 g, 80%). 1H NMR (500MHz, CDC1₃) (major isomer described): δ 7.66 (IH, s), 7.28-7.15 (5H, m), 7.04 (2H, m), 5.67 (IH, m), 4.94 (2H, m), 4.87 (IH, m), 4.25 (IH, d, / 8.6Hz), 4.17 (IH, m), 3.53 (IH, m), 3.22 (IH, m), 2.85 (IH, m), 2.01 (IH, m), 1.90 (IH, m), 1.78 (2H, m), 1.53 (2H, m), 1.36 (3H, d, /6.6Hz), 1.31 (IH, m). DESCRIPTION 24

(2i?,3R,4R,αR or S')-α-(3-Buten-l-yl)-2-{(li?)-l-[3,5- bis(trifluoromethyl)phenyl]ethosy}tetrahydro-3-phenyl-2H^'-pyran-4-methyl

Meihanesulfonate

Methanesulfonyl chloride (1.61 mL, 2.3 g, 21 mmol) was added dropwise to a stirred, cooled (-78 °C) solution of (2R,3R,4R,αR5)-α-(3-buten-l-yl)-2-{(lR)-l-[3,5- bis(trifluoromethyl)phenyl]ethoxy}tetrahydro-3-phenyl-2H-pyran-4-methanol (Description 23, 8:1 mixture of diastereoisomers; 3.5 g, 6.97 mmol) and triethylamine (5 mL, 3.5 g, 35 mmol) in dichloromethane (70 mL). The mixture was allowed lo warm to room temperature over 1.5 hours, then water (20 mL) and ethyl acetate (100 mL) were added. The layers were separated and the organic layer was washed with brine (100 mL). The combined aqueous layers were extracted with ethyl acetate (100 mL), the combined organic layers were dried (Na₂SO ), and the solvent was evaporated under reduced pressure. The residue was purified by column chromatography on silica gel, eluting with hexane/EtOAc (95:5 increasing to 80:20), to give the title compound (single diastereoisomer) as a colorless oil (2.76 g, 68%). 1H NMR (500MHz, CDC1₃): δ 7.65 (IH, s), 7.25 (3H, m), 7.14 (2H, s), 7.07 (2H, m), 5.62 (IH, m), 4.92 (3H, m), 4.39 (IH, t, 76.6Hz), 4.19 (IH, m), 4.17 (IH, d, 78.6Hz), 3.54 (IH, dt, 72.3, ll.OHz), 2.91 (3H, s), 2.80 (IH, dd, 78.3, 11.6Hz), 2.15 (IH, m), 1.90 (3H, m), 1.80 (IH, m), 1.68 (2H, m), 1.36 (3H, d, 76.6Hz).

DESCRIPTION 25

(2R5,5R or 5)-5-((2R,3R,4R)-{(lR)-l-[3,5-Bis(trifluoromethyl)phenyl]- ethoxy}tetrahydro-3-phenyl-2H-pyran-4-yl)-l,2,5-pentanetriol 5-

Methanesulfonate

2-Methyl-2-propanol (2 mL) was added to a solution of AD-mix-α (0.5 g) in water (2 mL) and the mixture was stirred vigorously at room temperature for 10 minutes. The yellow solution was then added to {2R,3R,4R, R or 5)-α-(3-buten-l-yl)-2-{(lR)- l-[3,5-bis(trifluoromethyl)phenyl]ethoxy}tetrahydro-3-phenyl-2H-ρyran-4-methyl methanesulfonate (Description 24; 0.24 g, 0.41 mmol) and the resulting mixture was stirred vigorously at room temperature overnight. Sodium sulfite (2 g) was added and the mixture was stirred for 30 minutes. Water (10 mL) and ethyl acetate (10 mL) were added, the layers were separated and the organic layer was washed with brine (100 mL). The combined aqueous layers were extracted with ethyl acetate (100 mL), the combined organic layers were dried (Na₂SO ), and the solvent was evaporated under reduced pressure. The residue was purified by column chromatography on silica gel, eluting with hexane/EtOAc (50:50 increasing to 10:90), to give the title compound (3:1 mixture of alcohol diastereoisomers) as a colorless oil (0.20 g, 79%). 1H NMR (500MHz, CD₃OD): δ 7.70 (IH, s), 7.30 (2H, s), 7.21 (3H, m), 7.17 (2H, m), 5.00 (IH, q, 76.5Hz), 4.33 (2H, m), 4.10 (IH, m), 3.65 (IH, m), 3.35 (3H, m), 3.03, 3.02 (3H, each s), 2.78 (IH, dd, / 8.3, 11.6Hz), 2.27 (IH, m), 1.90-1.40 (6H, m), 1.33 (3H, d, /6.6Hz).

DESCRIPTION 26

(2R5,5R or )-5-((2R,3R,4R)-{(lR)-l-[3,5-Bis(trifluoromethyl)phenyl]- ethoxy}tetrahydro-3-phenyl-2/7-pyran-4-yl)-l,2,5-pentanetriol 5-

Methanesulfonate

The title compound (1:3 mixture of alcohol diastereoisomers) was prepared from {2R,3R,4R, R or 5)-α-(3-buten-l-yl)-2-{ (lR)-l-[3,5-bis(trifluoromethyl)phenyl]- ethoxy}tetrahydro-3-phenyl-2H-pyran-4-methyl methanesulfonate (Description 24) according to the method of Description 25, using AD-mix-β instead of AD-mix-α. 1H NMR (500MHz, CD₃OD): δ 7.70 (IH, s), 7.30 (2H, s), 7.21 (3H, m), 7.17 (2H, m), 5.00 (IH, q, 76.5Hz), 4.33 (2H, m), 4.10 (IH, m), 3.65 (IH, m), 3.35 (3H, m), 3.03, 3.02 (3H, each s), 2.78 (IH, dd, 78.3, 11.6Hz), 2.27 (IH, m), 1.90-1.40 (6H, m), 1.33 (3H, d, /6.6Hz).

DESCRIPTION 27

(2RS,5R or 5)-5-((2R,3R,4R)-{(lR)-l-[3,5-Bis(trifluoromethyl)phenyl]- ethoxy}tetrahydro-3-phenyl-2H^r-pyran-4-yI)-l,2,5-pentanetriol l-(4-

Methylbensenesulfonate) 5-Methanesulfonate

4-Methylbenzenesulfonyl chloride (53 mg, 0.28 mmol) was added to a mixture of (2R«S,5R or 5)-5-((2R,3R,4R)-{(lR)-l-[3,5-bis(trifluoromethyl)ρhenyl]ethoxy}- tetrahydro-3-phenyl-2H-pyran-4-yl)-l,2,5-pentanetriol 5-methanesulfonate (3:l mixture of alcohol diastereoisomers; Description 25; 0.17 g, 0.28 mmol), dibutyltin oxide (1.4 mg, 5.6 μmol) and triethylamine (39 μL, 0.28 mmol) in dichloromethane (1.4 mL) and the mixture was stirred vigorously at room temperature for 3 hours. The solvent was evaporated under reduced pressure and the residue was purified by column chromatography on silica gel, eluting with hexane/EtOAc (80:20 increasing to 50:50), to give the title compound (3:1 mixture of alcohol diastereoisomers) as a colorless oil (0.142 g, 67%).

1H NMR (500MHz, CD₃OD): δ 7.75 (2H, d, 78.2Hz), 7.71 (IH, s), 7.40 (2H, d, 7 8.2Hz), 7.30 (2H, s), 7.20 (3H, m), 7.13 (2H, m), 5.00 (IH, q, 76.5Hz), 4.32 (IH, d, 7 8.5Hz), 4.26 (IH, t, 76.4Hz), 4.11 (IH, m), 3.85-3.75 (2H, m), 3.62 (2H, m), 3.47 (IH, m), 3.01, 3.00 (3H, each s), 2.74 (IH, dd, 78.3, 11.6Hz), 2.43 (3H, s), 2.20 (IH, m), 1.90-1.40 (6H, m), 1.33 (3H, d, 76.6Hz).

DESCRIPTION 28

(2R5,5R or S)-5-((2R,3R,4R)-{(lR)-l-[3,5-Bis(trifluoromethyI)phenyl]- ethoxy}tetrahydro-3-phenyl-2H-pyran-4-yl)-l,2,5-pentanetriol l-(4- MethylbenzenesuIfonate) 5-Methanesulfonate

The title compound (1:3 mixture of alcohol diastereoisomers) was prepared from {2RS,5R or S)-5-((2R,3R,4R)-{ (lR)-l-[3,5-bis(trifluoromethyl)phenyl]ethoxy }- tetrahydro-3-phenyl-2H-pyran-4-yl)-l ,2,5-pentanetriol 5-methanesulfonate (1:3 mixture of alcohol diastereoisomers; Description 26) according to the method of Description 27.

1H NMR (500MHz, CD₃OD): δ 7.75 (2H, d, / 8.2Hz), 7.71 (IH, s), 7.40 (2H, d, / 8.2Hz), 7.30 (2H, s), 7.20 (3H, m), 7.13 (2H, m), 5.00 (IH, q, /6.5Hz), 4.32 (IH, d, / 8.5Hz), 4.26 (IH, t, /6.4Hz), 4.11 (IH, m), 3.85-3.75 (2H, m), 3.62 (2H, m), 3.47 (IH, m), 3.01, 3.00 (3H, each s), 2.74 (IH, dd, / 8.3, 11.6Hz), 2.43 (3H, s), 2.20 (IH, m), 1.90-1.40 (6H, m), 1.33 (3H, d, /6.6Hz). DESCRIPTION 29 (2R,3R,4R)-2-{(lR)-l-[3,5-Bis(trifluoromethyl)phenyl]ethoxy}tetrahydro-4-

[{2R or iS)-oxiranyl]-3-phenyl-2M-pyran; and

(2^,3S₉4i?)-2-{(li?)-l-[3₉5-Bis(trifluorømethyl)pIιenyl]ethojiy}ietrahydrø-4- [(2S or i?)-oxiranyl]-3-phenyl-2fl^'-pyran (Isomers A and B) Dimethylsulfoxide (10 mL) was added to sodium hydride (60% dispersion in mineral oil, 385 mg, 9.6 mmol) and the mixture was stirred at room temperature for 30 minutes. Tetrahydrofuran (20 mL) was added and the mixture was cooled to -10 °C. Trimethylsulfonium iodide (2.13 g, 10.4 mmol) in dimethylsulfoxide (10 mL) was added and the mixture was stirred at 0 °C for 10 minutes. (2R,3R,4R)-2-{(lR)-l- [3,5-Bis(trifluoromethyl)phenyl]ethoxy}tetrahydro-3-phenyl-2H-pyran-4- carboxaldehyde (WO 00/56727A1; 3.58 g, 8.0 mmol) in tetrahydrofuran (10 mL) was added and the mixture was stirred at 0 °C for 30 minutes, then at room temperature for 30 minutes. Water (100 mL) was added and the mixture was extracted with ethyl acetate (3 x 100 mL). The combined organic fractions were washed with water (4 x 100 mL) and brine (100 mL), dried (MgSO₄) and the solvent was evaporated under reduced pressure. The residue was purified by column chromatography on silica gel, eluting with hexane/EtOAc (85:15 increasing to 80:20), to give (2R,3R,4R)- 2-{(lR)-l -[3,5-bis(trifluoromethyl)phenylJethoxy}tetrahydro-4-[(2R or S)-oxiranylJ- 3-phenyl-2H-pyran (Isomer A; single diastereoisomer; epoxide stereochemistry unassigned) as a colorless oil (1.37 g, 37%); 1H NMR (500MΗz, CDC1₃): δ 7.67 (IH, s), 7.23 (5H, m), 7.01 (2H, m), 4.97 (IH, q, 76.6Hz), 4.28 (IH, d, / 8.4Hz), 4.16 (IH, br d, / 11Hz), 3.53 (IH, br t, / 11Hz), 2.65 (IH, dd, / 8.4Hz), 2.60 (IH, m), 2.34 (IH, t, 74.5Hz), 1.95 (IH, dd, 74.5, 2.7Hz), 1.84 (IH, br d, / 11Hz), 1.68 (IH, m), 1.57 (IH, m), 1.37 (3H, d, / 6.6Hz); and

(2R,3R,4R)-2-{(lR)-l-[3,5-bis(trifluoromethyl)phenyl]ethoxyJtetrahydro-4-[(2S or R)- oxiranylJ-3-phenyl-2H-pyran (Isomer B; single diastereoisomer; epoxide stereochemistry unassigned) as a colorless oil (0.51 g, 14%); 1H NMR (500MHz, CDCI₃): δ 7.67 (IH, s), 7.27-7.20 (5H, m), 7.08 (2H, m), 4.96 (IH, q, 76.6Hz), 4.25 (IH, d, J 8.3Hz), 4.13 (IH, br d, / 12Hz), 3.54 (IH, br t, / 12Hz), 2.68 (IH, m), 2.61 (IH, dd, 7 11.5, 8.3Hz), 2.50 (IH, t, 74.6Hz), 2.46 (IH, dd, 74.6, 2.8Hz), 2.03 (IH, m), 1.60 (IH, br d, 7 12Hz), 1.49 (IH, m), 1.37 (3H, d, /6.6Hz); and a 1:1 mixture of Isomer A and Isomer B (1.16 g, 31%). DESCRIPTION 30

(2«,3R_?4«,α^ or S)-α-{[(2-Hydroxyethyl)thio]methyl}-2-{(l«)-l-[3,5- bis(trifluoromethyl)phenyl]ethoxy}tetrahydro-3-phenyl-2H^'-pyraπι-4-methanol; and

(2J?₅3^_?4_JK_s«S or «)-α-{[(2-hydroxyethyl)thio]methyl}-2-{(l«)-l-[3,5- bi§(trifluoromethyl)phenyl]ethoxy}tetrahydro-3-phenyϊ-2 -pyran-4-methanol

2-Mercaptoethanol (0.70 mL, 0.78 g, 10 mmol) was added to a degassed mixture of

(2R,3R,4R)-2-{(lR)-l-[3,5-bis(trifluoromethyl)phenyl]ethoxy}tetrahydro-4-

[(2R or 5)-oxiranyl]-3-phenyl-2H-pyran and (2R,3R,4R)-2-{(lR)-l-[3,5- bis(trifluoromethyl)-phenyl]ethoxy}tetrahydro-4-[(2₁S' orR)-oxiranyl]-3-phenyl-2H- pyran (Description 29, 1:1 mixture of diastereoisomers; 0.46 g, 1 mmol) and potassium hydroxide (0.56 g, 10 mmol) in propan-2-ol (10 mL) and the mixture was heated under reflux for 4 hours. The mixture was cooled and the solvent was evaporated under reduced pressure. Aqueous sodium hydroxide (IM, 20 mL) was added and the mixture was extracted with diethyl ether (3 x 20 mL). The combined organic fractions were washed with aqueous sodium hydroxide (IM, 2 x 20 mL) and brine (20 mL), dried (MgSO ) and the solvent was evaporated under reduced pressure to give the title compound (1:1 mixture of alcohol epimers) as a yellow oil (0.54 g,

100%).

1H NMR (500MHz, CDC1₃): δ 7.66 (IH, s), 7.26-7.23 (3H, m), 7.18 and 7.15 (2H, each s), 7.05 (2H, m), 4.95 (IH, m), 4.25, 4.23 (IH, each d, / 8.3Hz), 4.18 (IH, m),

3.62-3.53 (3H, m), 3.30 (IH, m), 2.90-1.51 (10H, m), 1.36 (3H, d, /6.6Hz).

DESCRIPTION 31 (2R,3R,4R,αR)-α-2-Propenyl-2-{(lR)-l-[3,5-bis(trifluoromethyI)phenyl]- ethoxy}tetrahydro-3-phenyl-2H-pyran-4-methanol

Allylmagnesium bromide (15.4 mL, 15.4 mmol) was slowly added to a stirred, cooled -40 °C solution of chlorobis[(15,2R,35',55')-2,6,6-trimethylbicyclo[3.1.1]hept-3- yl]borane (5.91 g, 18 mmol) in diethyl ether (20 mL). The mixture was warmed to room temperature and stirred for 1 hour before cooling to -78 °C. (2R,3R,4R)-2- {(lR)-l-[3,5-Bis(trifluoromethyl)phenyl]-ethoxy}tetrahydro-3-phenyl-2H-pyran-4- carboxaldehyde (WO 00/56727A1; 5.5 g, 12.3 mmol) in diethyl ether (10 mL) was slowly added and the mixture was stirred at -78 °C for 1.5 hours. The mixture was warmed to room temperature and stirred for a further 1.5 hours. The mixture was cooled to -78 °C and aqueous sodium acetate (3M, 20 mL) then aqueous hydrogen peroxide (31% wt, 10 mL) were added. The mixture was allowed to warm to room temperature and stirred overnight. The layers were separated and the aqueous layer was extracted with ethyl acetate (2 x 150 mL). The combined organic fractions were dried (MgSO ) and the solvent was evaporated under reduced pressure. The residue was purified by column chromatography on silica gel, eluting with EtOAc/hexane (90:10), to give the title compound as a yellow oil (4.7 g, 78%). 1H NMR (360MHz, CDC1₃): δ 1.25 (3H, d, 77Hz), 1.34 (IH, d, 76.6Hz), 1.54 (IH, d, 79Hz), 1.80 (IH, m), 2.05 (IH, m), 2.14 (IH, m), 2.88 (IH, m), 3.27 (IH, m), 3.53 (IH, m), 4.17 (IH, m), 4.25 (IH, d, / 8.6Hz), 4.94-4.98 (2H, m), 5.03 (IH, m), 5.60 (IH, m), 7.03-7.05 (2H, m), 7.18-7.24 (5H, m), 7.65 (IH, s).

DESCRIPTION 32 (2R,3R,4R,αS)-α-2-Propenyl-2-{(lR)-l-[3,5-bis(trifluoromethyl)phenyl]- ethoxy}tetrahydro-3-phenyl-2/7-pyran-4-methanol

Prepared from (2R,3R,4R)-2-{ (lR)-l-[3,5-bis(trifluoromethyl)phenyl]ethoxy}- tetrahydro-3-phenyl-2H-pyran-4-carboxaldehyde (WO 00/56727A1), allylmagnesium bromide and chlorobis[(lR,2S,3R,5R)-2,6,6-trimethylbicyclo[3.1.1]hept-3-yl]borane according to the method of Description 31.

1H NMR (360MHz, CDC1₃): δ 1.36 (3H, d, /6.6Hz), 1.58 (IH, m), 1.80 (IH, m), 1.95 (IH, m), 2.17 (IH, m), 2.23 (IH, m), 2.55 (IH, m), 3.30 (IH, m), 3.55 (IH, m), 4.17 (IH, m), 4.23 (IH, d, /8.2Hz), 4.94 (IH, m), 5.03-5.06 (2H, m), 5.59 (IH, m), 7.03- 7.04 (2H, m), 7.05 (2H, m), 7.16-7.24 (3H, m), 7.69 (IH, s), 7.85 (IH, s).

DESCRIPTION 33

(lS)-l-((2fi,3i?,4^)-{(l^)-l-[3,5-Bis(trifluoromethyl)phenyl]ethøxy}-tetrahydro- 3-phenyl-2fl^r-pyran-4-yl)-l,3-propanediol

(2R,3R,4R,αR)-α-2-Proρenyl-2-{(lR)-l-[3,5-bis(trifluoromethyl)phenyl]ethoxy}- tetrahydro-3-phenyl-2H-pyran-4-methanol (Description 31; 1.0 g, 2 mmol) was dissolved in dichloromethane/methanol (1:1, 30 mL), cooled to -78 °C and purged with nitrogen and then oxygen. Ozone was bubbled through the mixture for 15-20 minutes, until a blue coloration persisted. The mixture was purged with oxygen, then nitrogen, warmed to 0 °C and sodium borohydride (0.3 g, 8 mmol) was added in portions. The mixture was stirred at 0 °C for 30 minutes, aqueous ammonium chloride (10%, 40 mL) was added and the mixture was extracted with dichloromethane (2 x 75 mL). The combined organic fractions were dried (MgSO₄) and the solvent was evaporated under reduced pressure. The residue was purified by column chromatography on silica gel, eluting with EtOAc/hexane (50:50) to give the title compound as a colorless oil (0.5 g, 50%).

1H NMR (500MHz, CD₃OD): δ 1.32 (3H, d, 76.6Hz), 1.41 (IH, m), 1.55 (IH, m), 1.62 (IH, m), 1.75 (IH, m), 1.86 (IH, m), 2.81 (IH, dd, 78.6, 11.5Hz), 3.45-3.51 (2H, m), 3.62 (IH, dt, J_d 2.3, /_t 12.0Hz), 4.1 (IH, m), 4.36 (IH, d, / 8.7Hz), 4.99 (IH, q, / 6.5Hz), 7.09-7.11 (2H, m), 7.16-7.21 (3H, m), 7.31 (2H, s), 7.70 (IH, s).

DESCRIPTION 34 (15)-l-((2R,3R,4R)-{(lR)-l-[3,5-Bis(trifluoromethyl)phenyl]ethoxy}-tetrahydro-3- phenyl-2/7-pyran-4-yl)-l,3-propanediol

Prepared from (2R,3R,4R,αS)-α-2-propenyl-2-{(lR)-l-[3,5- bis(trifluoromethyl)phenyl]ethoxy}tetrahydro-3-phenyl-2H-pyran-4-methanol

(Description 32) according to the method of Description 33.

1H NMR (360MHz, CDC1₃): δ 1.35 (3H, d, / 6.6Hz), 1.57-1.60 (3H, m), 1.80 (IH, m),

1.95 (IH, m), 2.06 (IH, m), 2.43 (IH, m), 2.50 (IH, m), 3.54-3.57 (3H, m), 3.80 (IH, m), 4.23 (IH, m), 4.24 (IH, d, / 8.1Hz), 4.93 (IH, m), 7.02-7.04 (2H, m), 7.16-7.23

(5H, m), 7.65 (IH, s).

DESCRIPTION 35 (lR)-l-((2R,3R,4R)-{(lR)-l-[3,5-Bis(trifluoromethyl)phenyl]ethoxy}-tetrahydro- 3-phenyl-2HT-pyran-4-yl)-l,3-propanediol Dimethylsulfonate

(lR)-l-((2R,3R,4R)-{(lR)-l-[3,5-Bis(trifluoromethyl)phenyl]ethoxy}tetrahydro-3- phenyl-2H-pyran-4-yl)-l,3-propanediol (Description 33; 0.5 g, 1 mmol) was dissolved in dichloromethane (5 mL) and cooled to -10 °C. Triethylamine (0.56 mL, 4 mmol) was added followed by methanesulfonyl chloride (0.23 mL, 3 mmol) dropwise. The mixture was stirred at -20 °C for 30 minutes, then at room temperature for 15 minutes. Water (75 mL) was added and the mixture was extracted with dichloromethane (3 x 75 mL). The combined organic fractions were dried (MgSO₄) and the solvent was evaporated under reduced pressure to give the title compound as a colorless foam (0.65 g, 100%).

1H NMR (500MHz, CDC1₃): δ 1.35 (3H, d, 76.7Hz), 1.65 (IH, m), 1.77 (IH, m), 2.05 (IH, m), 2.15-2.20 (2H, m), 2.75 (IH, m), 2.84 (3H, s), 2.92 (3H, s), 3.55 (IH, t, 7 12Hz), 4.15-4.22 (4H, m), 4,55 (IH, m), 4.92 (IH, q, 76.5Hz), 7.09-7.13 (4H, m), 7.24-7.26 (3H, m), 7.65 (IH, s).

DESCRIPTION 36 (lS)-l-((2_J-?,3fi,4fi)-{(lS)-l-[3₅5-Bis(trifluoromethyl)phenyl]ethoxy}-tetrahydro-3- phenyl-2J7-pyran-4-yI)-l,3-propanediol DimethyIsuIfbnate Prepared from (lS)-l-((2R,3R,4R)-{ (lR)-l-[3,5-bis(trifluoromethyl)phenyl]- ethoxy } tetrahydro-3 -phenyl-2H-pyran-4-yl)- 1 ,3 -propanediol (Description 34) according to the method of Description 35.

1HNMR (400MHz, CDC1₃): δ 1.35 (3H, d, 76.6Hz), 1.60 (IH, m), 1.80-1.90 (2H, m), 1.99 (IH, m), 2.48 (IH, m), 2.55 (IH, m), 2.72 (3H, s), 2.92 (3H, s), 3.60 (IH, m), 4.1 (IH, m), 4.20-4.22 (3H, m), 4.45 (IH, m), 4.48 (IH, m), 7.08-7.14 (4H, m), 7.26-7.29 (3H, m), 7.66 (IH, s).

DESCRIPTION 37 Methyl (2R,3R,4R)-2-{(lR)-l-[3,5-Bis(trifluoromethyl)phenyl]ethoxy}- tetrahydro-3-phenyl-2J7-pyran-4-carboxylate

Trimethylsilyldiazomethane (2.0M in hexanes, 3.5 mL, 7 mmol) was added in portions over 1 hour to a solution of (2R,3R,4R)-2-{(lR)-l-[3,5- bis(trifluoromethyl)phenyl]ethoxy}tetrahydro-3-phenyl-2H-pyran-4-carboxylic acid (WO 00/56727A1; 3.2 g, 6.9 mmol) in a mixture of methanol (10 mL) and toluene (7 mL). The solvent was evaporated under reduced pressure and the residue was purified by column chromatography on silica gel, eluting with EtOAc/hexane (80:20), to give the title compound as a colorless solid (2.4 g, 73%).

1H NMR (500MHz, CDC1₃): δ 7.67 (IH, s), 7.22 (5H, m), 7.07 (2H, m), 4.96 (IH, q, / 6.6Hz), 4.28 (IH, d, 78.2Hz), 4.17 (IH, br d, 71 lHz), 3.58 (IH, br t, / 11Hz), 3.45 (3H, s), 3.04 (IH, dd, / 8.2, 11.4Hz), 2.81 (IH, br t, / 11Hz), 1.94 (IH, br q, / 11Hz), 1.86 (IH, br d, / 11Hz), 1.38 (3H, d, 76.6Hz). DESCRIPTION 38

(2fi₅3^,4«)-«,κ-Di-(2-propenyl)-2-{(l«)-l-[3_?5-bis(trϊfiuoromethyl)phenyl]- ethoxy}tetrahydro-3-phenyl-2 -pyran-4-methanol

Allylmagnesium bromide (l.OM in hexanes, 8.8 mL, 8.8 mmol) was added slowly to ; stirred, cooled (0 °C) solution of methyl (2R,3R,4R)-2-{(lR)-l-[3,5- bis(trifluoromethyl)phenyl]ethoxy}letrahydro-3-phenyl-2H-pyran-4-carboxylate (Description 37; 1.4 g, 2.9 mmol) in tetrahydrofuran (10 mL). The mixture was warmed to room temperature and stirred for 30 minutes. Saturated aqueous ammonium chloride (50 mL) was added and the mixture was extracted with ethyl acetate (3 x 30 mL). The combined organic fractions were dried (MgSO₄) and the solvent was evaporated under reduced pressure. The residue was purified by column chromatography on silica gel, eluting with EtOAc/hexane (80:20), to give the title compound as a colorless oil (1.48 g, 95%).

1H NMR (400MHz, CDC1₃): δ 7.66 (IH, s), 7.22 (3H, m), 7.16 (2H, s), 7.11 (2H, m), 5.88-5.70 (2H, m), 5.08-4.97 (4H, m), 4.89 (IH, q, / 6.6Hz), 4.22 (IH, d, / 7.7Hz), 4.15-4.09 (IH, m), 3.50 (IH, br t, / 12Hz), 2.84 (IH, dd, / 10.9, 7.7Hz), 2.21-2.11 (5H, m), 1.78 (IH, br d, / 12Hz), 1.64 (IH, br q, / 12Hz), and 1.35 (3H, d, 76.6Hz).

DESCRIPTION 39 3-((2R,3R,4R)-{(lR)-l-[3,5-Bis(trifluoromethyl)phenyl]ethoxy}tetrahydro-3- phenyl-2H-pyran-4-yl)-l,3,5-propanetriol

(2R,3R,4R)-α,α-Di-(2-propenyl)-2-{(lR)-l-[3,5-bis(trifluoromethyl)phenyl]ethoxy}- tetrahydro-3-phenyl-2H-pyran-4-methanol (Description 39; 1.48 g, 2.8 mmol) was dissolved in dichloromethane/methanol (1:1, 30 mL), cooled to -78 °C and purged with nitrogen and then oxygen. Ozone was bubbled through the mixture for 15-20 minutes, until a blue coloration persisted. The mixture was purged with oxygen, then nitrogen, warmed to 0 °C and sodium borohydride (0.21 g, 5.6 mmol) was added in portions. The mixture was stirred at 0 °C for 30 minutes, aqueous ammonium chloride (10%, 50 mL) was added and the mixture was extracted with dichloromethane (2 x 50 mL). The combined organic fractions were dried (MgSO₄) and the solvent was evaporated under reduced pressure. The residue was purified by column chromatography on silica gel, eluting with EtOAc/hexane (50:50), to give the title compound as a colorless oil (0.80 g, 55%).

1H NMR (400MHz, CDC1₃): δ 7.66 (IH, s), 7.19 (3H, m), 7.14 (2H, s), 7.04 (2H, m), 4.89 (IH, q, 76.6Hz), 4.25 (IH, d, 78.0Hz), 4.17 (IH, br d, 71 lHz), 3.80 (2H, br s), 3.70(1H, br m), 3.56 (2H, m), 3.40 (IH, s), 3.24 (IH, br s), 2.84 (IH, br s), 2.72 (IH, dd, 711.3, 8.0Hz), 2.37 (IH, br t, 711Hz), 1.89-1.80 (2H, m), 1.70-1.56 (3H, m), 1.34 (3H, d, 76.6Hz), 1.19-1.13 (IH, m).

DESCRIPTION 40 3-((2^,3i?,4fi)-{(li?)-l-[3,5-Bϊs(trifluøromethyl)phenyl]ethoxy}tetrahydro-3- phenyl-2/7-pyran-4-yl)-l,3,5-propanetriol l,5-DimethyIsulfonate Methanesulfonyl chloride (0.24 mL, 0.36 g, 3.2 mmol) was added slowly to a stirred, cooled (-10 °C) solution of 3-((2R,3R,4R)-{(lR)-l-[3,5- bis(trifluoromethyl)phenyl]ethoxy}tetrahydro-3-phenyl-2H-pyran-4-yl)-l,3,5- propanetriol (Description 39; 0.80 g, 1.5 mmol) and triethylamine (0.48 mL, 0.35 g, 3.5 mmol) in dichloromethane (8 mL). The mixture was stirred at -10 °C for 20 minutes, then water (25 mL) was added and the mixture was extracted with dichloromethane (3 x 30 mL). The combined organic fractions were washed with aqueous citric acid (10%, 30 mL) and saturated aqueous sodium carbonate (30 mL), dried (MgSO ) and the solvent was evaporated under reduced pressure to give the title compound as a colorless foam (1.0 g, 100%).

1H NMR (500MHz, CDC1₃): δ 7.67 (IH, s), 7.28 (3H, m), 7.13 (4H, m), 4.90 (IH, q, 7 6.6Hz), 4.34-4.15 (6H, m), 3.68 (IH, s), 3.58 (IH, br t, / 11Hz), 2.97 (3H, s), 2.95 (3H, s), 2.78 (IH, m), 2.22 (IH, br t, 711Hz), 1.97-1.94 (3H, m), 1.83-1.76 (2H, m), 1.61 (IH, m), 1.35 (3H, d, 76.6Hz).

DESCRIPTION 41

(2^,3^,4fi)-2-{(lfi)-l-[3,5-Bis(trifluoromethyl)phenyl]ethoxy}tetrahydro-4- [(2R or iS)-oxiranyl]-3-phenyl-2fi^r-pyran; and

(2«,3i?,4«)-2-{(li?)-l-[3,5-Bi§(trifluoromethyl)phenyl]ethoxy}tetralιydro-4- [{2S or i?)-oxiranyl]-3-phenyl-2i?-pyran (Isomers A and B) Dimethylsulfoxide (10 mL) was added to sodium hydride (60% dispersion in mineral oil, 385 mg, 9.6 mmol) and the mixture was stirred at room temperature for 30 minutes. Tetrahydrofuran (20 mL) was added and the mixture was cooled to -10 °C. Trimethylsulfonium iodide (2.13 g, 10.4 mmol) in dimethylsulfoxide (10 mL) was added and the mixture was stirred at 0 °C for 10 minutes. (2R,3R,4R)-2-{(lR)-l- [3,5-Bis(trifluoromethyl)phenyl]ethoxy}tetrahydro-3-phenyl-2H-pyran-4- carboxaldehyde (WO 00/56727A1; 3.58 g, 8.0 mmol) in tetrahydrofuran (10 mL) was added and the mixture was stirred at 0 °C for 30 minutes, then at room temperature for 30 minutes. Water (100 mL) was added and the mixture was extracted with ethyl acetate (3 x 100 mL). The combined organic fractions were washed with water (4 x 100 mL) and brine (100 mL), dried (MgSO ) and the solvent was evaporated under reduced pressure. The residue was purified by column chromatography on silica gel, eluting with hexane/EtOAc (85:15 increasing to 80:20), to give: (2R,3R,4R)-2-{(IR)-l-[3,5-bis(trifluoromethyl)phenyl]ethoxyjtetrahydro-4-[(2R oτ S)- oxiranyl]-3-phenyl-2H-pyran (Isomer A; single diastereoisomer; epoxide stereochemistry unassigned) as a colorless oil (1.37 g, 37%); 1H NMR (500MHz, CDC1₃): δ 7.67 (IH, s), 7.23 (5H, m), 7.01 (2H, m), 4.97 (IH, q, /6.6Hz), 4.28 (IH, d, / 8.4Hz), 4.16 (IH, br d, / 1 IHz), 3.53 (IH, br t, / 1 IHz), 2.65 (IH, dd, / 8.4Hz), 2.60 (IH, m), 2.34 (IH, t, /4.5Hz), 1.95 (IH, dd, /4.5, 2.7Hz ), 1.84 (IH, br d, / 11Hz), 1.68 (IH, m), 1.57 (IH, m), 1.37 (3H, d, /6.6Hz); and

(2R,3R,4R)-2-{(lR)-l-[3,5-bis(trifluoromethyl)phenyl]ethoxy}tetrahydro-4-[(2S or R)- oxiranyl]-3-phenyl-2H-pyran (Isomer B; single diastereoisomer; epoxide stereochemistry unassigned) as a colorless oil (0.51 g, 14%); 1H NMR (500MHz, CDCI₃): δ 7.67 (IH, s), 7.27-7.20 (5H, m), 7.08 (2H, m), 4.96 (IH, q, /6.6Hz), 4.25 (IH, d, / 8.3Hz), 4.13 (IH, br d, / 12Hz), 3.54 (IH, br t, / 12Hz), 2.68 (IH, m), 2.61 (IH, dd, / 11.5, 8.3Hz), 2.50 (IH, t, /4.6Hz), 2.46 (IH, dd, /4.6, 2.8Hz), 2.03 (IH, m), 1.60 (IH, br d, / 12Hz), 1.49 (IH, m), 1.37 (3H, d, / 6.6Hz); and a 1:1 mixture of Isomer A and Isomer B (1.16 g, 31%).

EXAMPLE 1 (4«)-8-[(25,3i?,4i?)-2-[(lK)-l-(3₅5-Bis(trifluoromethyl)phenyl)ethoxy]-3-(3- bromo-4-fluorophenyl)tetrahydropyran-4-yI]metlιyl-2-oxa-8-a3a- spiro[4.5]decan-4-ol (lS,4R)-4,7,7-Trimethyl-3-oxo-2-oxa-bicyclo[2.2.1 ]heρtane-l-carboxylic acid (4R)-8- {(2R,3R,4R)-2-[(lR)-l-(3,5-bis(trifluoromethyl)phenyl)ethoxy]-3-(3-bromo-4- fluoroρhenyl)-tetrahydropyran-4-ylmethyl}-2-oxa-8-aza-spiro[4.5]dec-4-yl ester (Description 14; 130mg, 0.15mmol) was dissolved in dichloromethane (3ml) and the solution was cooled to -78°C under nitrogen. A solution of diisobutylaluminium hydride (0.9ml, IM in toluene) was added dropwise and the solution was stirred for 30min. Methanol (0.1ml) was added dropwise and the resulting quenched solution was poured carefully onto sodium hydroxide (10ml, 4M). This was stirred for 30min. The organic extracts were pooled, washed with brine, dried (MgSO₄) and concentrated in vacuo. The residue was purified by chromatography on silica gel using 1-5% methanol in dichloromethane as eluant. This afforded the product as a white foam (96mg, 93%).

This was further purified by recrystallisation of the HCI salt from ether- dichloromethane to give the product as white crystals (86mg). 1H NMR (500MHz, CDC1₃): δ 7.69 (IH, s), 7.23 (IH, dd, 72.1, 6.5Hz), 7.21 (2H, s), 6.98 (IH, t, 78.3Hz), 6.89-6.92 (IH, m), 4.95(1H, q, 76.5Hz), 4.10-4.14 (2H, m), 4.03 (IH, dd, 74.6, lO.OHz), 3.90 (IH, dd, 7 1.7, 4.4Hz), 3.65 (IH, dd, /2.2, lO.OHz), 3.59 (IH, d, / 8.5Hz), 3.54 (IH, d, / 8.5Hz), 3.51 (IH, dd, / 12.1, 2.0Hz), 2.39-2.37 (IH, m), 2.37 (IH, dd, / 8.3, ll.OHz), 2.28-2.20 (IH, m), 2.12-1.84 (6H, m), 1.73 - 1.68 (IH, m), 1.49-1.43 (2H, m), 1.39 (3H, d, /6.6Hz); m/z (ES+) 684 (M⁺+1, 95%), 686 (M⁺+1, 100%).

EXAMPLE 2 (4RS)-8-[(2R,3R,4R)-2-[(lR)-l-(3,5-Bis(trifluoromethyl)phenyl)ethoxy]-3-phenyl- tetrahydropyran-4-yl]methyl-2-oxa-8-aza-spiro[4.5]decan-4-ol

Triethylamine (140μL, l.Ommol) was added to a suspension of 2-oxa-8-aza- spiro[4.5]decan-4-ol hydrochloride (Description 8; O.lg, 0.54mmol) in 1,2-dichloroethane (2ml). After 10 minutes, (2R,3R,4R)-2-[(lR)-l-(3,5- bis(trifluoromethyl)phenyl)ethoxy]-3-phenyl-tetrahydropyran-4-carbaldehyde (see Example 43 in WO 00/56727; 0.24g, 0.54mmol) was added as a solution in 1,2- dichloroethane (3ml). The mixture was stirred at room temperature for 30 minutes then sodium triacetoxyborohydride (0.2g, 0.94mmol) was added. The reaction was stirred at room temperature overnight. The mixture was partitioned between dichloromethane and brine. The organic layer was dried (MgSO₄) and concentrated in vacuo. The residue was purified by preparative thin layer chromatography, eluting with 5% methanol/dichloromethane, to give the title compound (72mg, 23%). 1H NMR (400 MHz, CDC1₃): δ 7.58 (IH, s), 7.16-7.15 (3H, m), 7.09 (2H_S s), 6.95- 6.92 (2H, m), 4.87 (IH, q, 76.6Hz), 4.12 (IH, d, 78.3Hz), 4.07 (IH, dd, 73.5, 11.7Hz), 3.96-3.93 (IH, m), 3.83-3.81 (IH, m), 3.59-3.43 (4H, m), 2.41-2.38 (IH, m), 2.34-2.29 (IH, m), 2.21-2.15 (IH, m), 2.05-1.85 (5H, m), 1.74-1.66 (2H, m), 1.45- 1.34 (4H, m), 1.29 (3H, d, 76.6Hz).

EXAMPLE 3 (45)-8-[(2R,3R,4R)-2-[(lR)-l-(3,5-Bis(trifluoromethyl)phenyl)ethoxy]-3-(4- fluorophenyl)-tetrahydropyran-4-yl]methyl-2-oxa-8-aza-spiro[4.5]decan-4-ol

A solution of (15,4R)-4,7,7-trimethyl-3-oxo-2-oxa-bicyclo[2.2.1]heptane-l-carboxylic acid (4RS)-8-{(2R,3R,4R)-2-[(lR)-l-(3,5-bis(trifluoromethyl)phenyl)ethoxy]-3-(4- fluorophenyl)-tetrahydropyran-4-ylmethyl}-2-oxa-8-aza-spiro[4.5]dec-4-yl ester (Description 12; 138mg, 0.175mmol) in dichloromethane (5ml) was cooled to -78°C and diisobutylaluminium hydride (1.5M in toluene, 0.5ml, 0.75mmol) added. The reaction was stirred for 30 minutes then quenched by the addition of 2M aqueous sodium hydroxide solution. The mixture was allowed to warm to room temperature then dried over Na₂SO₄ and filtered through Celite™. The filtrate was concentrated in vacuo and the residue purified by preparative thin layer chromatography, eluting with 7% methanol/dichloromethane to give the title compound (27mg, 26%). 1H NMR (360 MHz, CDC1₃): δ 7.67 (IH, s), 7.17 (2H, s), 7.00-6.92 (4H, m), 4.95 (IH, q, 76.5Hz), 4.15-4.11 (2H, m), 4.04-4.00 (IH, m), 3.90-3.89 (IH, m), 3.67-3.60 (2H, m), 3.54-3.48 (2H, m), 2.52-2.42 (IH, m), 2.41-2.36 (IH, m), 2.34-2.14 (2H, m), 2.10-1.87 (4H, m), 1.86-1.76 (IH, m), 1.75-1.67 (IH, m), 1.55-1.40 (4H, m), 1.36 (3H, d, 76.5Hz); m/z (ES+) 606 (M+H) ⁺. EXAMPLE 4 (4R)-8-[(2R,3R,4R)-2-[(lR)-l-(3,5-Bis(trifluoromethyl)phenyl)ethoxy]-3-(4- fluorophenyl)-tetrahydropyran-4-yl]methyl-2-oxa-8-aza-spiro[4.5]decan-4-ol

The title compound was prepared from (15,4R)-4,7,7-trimethyl-3-oxo-2-oxa- bicyclo[2.2. l]heρtane-l-carboxylic acid (4R)-8-{ (2R,3R,4R)-2-[(lR)-l-(3₃5- bis(trifluoromethyl)phenyl)ethoxy]-3-(4-fluorophenyl)-tetrahydropyran-4-ylmethyl}- 2-oxa-8-aza-spiro[4.5]dec-4-yl ester (Description 13) according to the method of Example 3.

1H NMR (360 MHz, CDC1₃): δ 7.67 (IH, s), 7.17 (2H, s), 7.03-6.89 (4H, m), 4.95 (IH, q, 76.6Hz), 4.13 (2H, m), 4.02 (IH, dd, 74.7, 9.8Hz), 3.90 (IH, m), 3.65 (IH, dd, J 2.0, 10.2Hz), 3.59 (IH, d, / 8.2Hz), 3.53 (IH, dd, / 8.6Hz), 3.50 (IH, dd, / 1.6, 12.1Hz), 2.57-1.52 (11H, m), 1.60-1.40 (4H, m), 1.36 (3H, d, 76.5Hz); m/z (ES+) 606 (M+H)⁺.

EXAMPLE 5 (4RS)-8-[(2R,3R,4R)-2-[(lR)-l-(3,5-Bis(trifluoromethyl)phenyl)ethoxy]-3-(3,4- difluorophenyl)-tetrahydropyran-4-yl]methyl-2-oxa-8-aza-spiro[4.5]decan-4-ol

A mixture of methanesulfonic acid [(2R,3R,4R)-2-[(lR)-l-(3,5-bis(trifluoromethyl)- phenyl)ethoxy]-3-(3,4-difluorophenyl)-tetrahydropyran-4-yl]methyl ester (Description 16; 0.275g, 0.49mmol), 2-oxa-8-aza-spiro[4.5]decan-4-ol hydrochloride (Description 8; O.lg, 0.37mmol), potassium carbonate (237g, 1.7mmol) and acetonitrile (3ml) was stirred at 55°C overnight and then at 65°C for 18 hours. The mixture was treated with water and extracted into dichloromethane. The combined organic extracts were dried (Na₂SO₄) and concentrated. The residue was purified by preparative TLC (dichloromethane: methanol) to give the title compound. m/z (ES+) 624 (M+H)⁺.

EXAMPLE 6 8-[(2i?_?3^,4i?)-2-[(l«)-l-(3_?5-Bis(trifluoromethyl)phenyl)ethoxy]-3-(4- fluorophenyl)tetrahydropyran-4-yl]methyl-2-oxa-8-a2a-spiro[4.5]decane A mixture of 2-[l-{(2R,3R,4R)-2-[(lR)-l-(3,5-bis(trifluoromethyl)phenyl)ethoxy]-3- (4-fluorophenyl)-tetrahydropyran-4-carbaldehyde (Description 11; 0.15g, 0.32mmol), 2-oxa-8-azaspiro[4.5jdecane hydrochloride (0.85g, 0.48mmol), triethylamine (50μL, 0.49mmol), sodium triacetoxyborohydride (0.136g, 0.64mmol) and dichloroethane (3ml) was stirred at room temperature for 2 hours. The mixture was treated with sodium bicarbonate and extracted into dichloromethane. The combined organic extracts were dried (Na₂SO ) and concentrated. The residue was purified by chromatography on alumina (grade HI) using ethyl acetate in hexane (10-50%) to give the title compound.

1H NMR (360 MHz, CDC1₃): δ 1.36 (3H, d, /6.6Hz), 1.38-1.55 (5H, m), 1.61 (2H, t, / 7.1Hz), 1.82-2.05 (6H, m), 2.18-2.31 (2H, m), 2.37 (IH, dd, 710.5, 8.4Hz), 3.43 (2H, s), 3.51 (IH, br t), 3.77 (2H, t, 77.1Hz), 4.10-4.14 (2H, m), 4.95 (IH, q, 6.6Hz), 6.91-7.01 (4H, m), 7.17 (2H, s), 7.67 (IH, s); m/z (ES+) 591 (M⁺+H, 100%).

EXAMPLE 7- 8-[(2R,35,45)-2-[(lR)-l-(3,5-Bis(trifluoromethyl)phenyl)ethoxy]-3- phenyl(tetrahydropyran-4-yl)]methyl-l,l-dimethyl-2-oxa-8-aza-spiro[4.5]decane

A mixture of (2R,35,45)-2-[(lR)-l-(3,5-bis(trifluoromethyl)phenyl)ethoxy]-3- phenyl(tetrahydropyran)-4-aldehyde (see Example 43 in WO 00/56727) and 1,1- dimethyl-2-oxa-8-aza-spiro[4.5]decane {Bioorg. and Med. Chem. Lett. 2002, 12, 1759) was reacted and purified according to the procedure described in Example 6 to give the title compound.

1H NMR (360 MHz, CDC1₃): δ 1.07 (3H, s), 1.08 (3H, s), 1.20-1.37 (3H, m), 1.46 (3H, d, 76.6Hz), 1.50-1.64 (3H, m), 1.78-1.91 (3H, m), 2.0-2.15 (3H, m), 2.57-2.71 (3H, m), 2.97-3.02 (IH, m), 3.72-3.79 (3H, m), 4.04-4.10 (IH, m), 4.41 (IH, d, 3.2Hz), 4.95 (IH, q, 76.6Hz), 7.24-7.26 (5H, m), 7.36 (2H, s), 7.67 (IH, s); m/z (ES+) 600 (M⁺+H, 100%)

EXAMPLE 8 8-[(2R,3S,45)-2-[(lR)-l-(3,5-Bis(trifluoromethyl)phenyl)ethoxy]-3- phenyI(tetrahydropyran-4-yl)]methyl-2,2-dimethyI-l-oxa-8-asa-spiro[4.5]decane A mixture of (2R,35,45)-2-[(lR)-l-(3,5-bis(trifluoromethyl)phenyl)ethoxy]-3- phenyl(tetrahydropyran)-4-aldehyde (see Example 43 in WO 00/56727) and 2,2- dimethyl-l-oxa-8-aza-spiro[4.5]decane (Description 18) was reacted and purified according to the procedure described in Example 6 to give the title compound. 1H NMR (360 MHz, CDC1₃): δ 1.20 (6H, s), 1.44 (3H, d, /6.6Hz), 1.46-1.55 (4H, m), 1.60-1.71 (IH, m), 1.72-1.82 (4H, m), 1.82-1.95 (IH, m), 1.95-2.18 (3H, m), 2.25- 2.38 (2H, m), 2.50-2.65 (3H, m), 3.73-3.75 (IH, m), 3.97-4.04 (IH, m), 4.41 (IH, d, 2.3Hz), 4.87 (IH, q, 76.6Hz), 7.18 (2H, s) 7.18-7.32 (5H, m), 7.58 (IH, s); m z (ES+) 600 (M⁺+H, 100%)

EXAMPLE 9> 8-[(2ff,3S,4S)-2-[(l^)-l-(3₅5-Bi§(trifluorømethyl)phenyl)ethoxy]-3-ρhenyl- tetrahydropyran-4-yl]methyl-l-oxa-8-a-.a-spiro[4.5]decane A mixture of (2R,35',4S)-2-[(lR)-l-(3,5-bis(trifluoromethyl)ρhenyl)ethoxy]-3- phenyl(tetrahydropyran)-4-aldehyde (see Example 43 in WO 00/56727) and l-oxa-8- aza-spiro[4.5]decane (Description 17) was reacted and purified according to the procedure described in Example 6 to give the title compound. 1H NMR (360 MHz, CDCI₃): δ 1.45 (3H, d, 76.6Hz), 1.46-1.70 (7H, m), 1.86-2.32 (7H, m), 2.40-2.75 (4H, m), 3.73-3.78 (3H, m), 4.04-4.10 (IH, m), 4.41 (IH, d, 3.0Hz), 4.95 (IH, q, 76.6Hz), 7.18-7.29 (5H, m) 7.35 (2H, s), 7.67 (IH, s); m/z (ES+) 572 (MT+H, 100%).

EXAMPLE 10

8-[(2R,35,4S)-2-[(lR)-l-(3,5-Bis(trifluoromethyl)phenyl)ethoxy]-3-phenyl- tetrahydropyran-4-yl]methyl-2-oxa-8-aza-spiro[4.5]decane

A mixture of (2R,35,4₁S)-2-[(lR)-l-(3,5-bis(trifluoromethyl)ρhenyl)ethoxy]-3- phenyl(tetrahydropyran)-4-aldehyde (see Example 43 in WO 00/56727) and 2-oxa-8- aza-spiro[4.5]decane {Bioorg. and Med. Chem. Lett. 2002, 12, 1759) was reacted and purified according to the procedure described in Example 6 to give the title compound.

1H NMR (360 MHz, CDC1₃): δ 1.45 (3H, d, /6.6Hz), 1.48-1.70 (5H, m), 1.66 (2H, t, / 7.1Hz), 1.86-1.98 (IH, m), 2.01-2.20 (4H, m), 2.36-2.48 (2H, m), 2.56 (IH, dd, / 11.9, 3.0Hz), 2.58-2.70 (IH, m), 3.48 (2H, s), 3.75 (IH, dd, 11.1, 3.7Hz), 3.81 (2H, t, 77.1Hz), 3.97-4.04 (IH, m), 4.42 (IH, d, 73.0Hz), 4.87 (IH, q, 76.6Hz), 7.18 (2H, s), 7.19-7.29 (5H, m), 7.59 (IH, s); m/z (ES+) 572 (M⁺+H, 100%). EXAMPLE 11

8-[(2R,3R,4R)-2-[(lR)-l-(3,5-Bis(trifluoromethyl)phenyl)ethoxy]-3-phenyl- tetrahydropyran-4-yl]methyl-2-oxa-8-asa-spiro[4.5]decane

A mixture of (2R,3R,4R)-2-[(lR)-l-(3,5-bis(trifluoromethyl)phenyl)ethoxy]-3- phenyl(tetrahydropyran)-4-aldehyde (see Example 45 in WO 00/56727) and 2-oxa-8- aza-spiro[4.5]decane {Bioorg. and Med. Chem. Lett. 2002, 12, 1759) was reacted and purified according to the procedure described in Example 6 to give the title compound.

1H NMR (360 MHz, CDC1₃): δ 1.35 (3H, d, 76.6Hz), 1.40-1.50 (3H, m), 1.60 (2H, t, 7

7.1Hz), 1.85-2.05 (6H, m), 2.20-2.42 (3H, m), 3.42 (2H, s), 3.52 (IH, multiplet centre

(mc)), 3.77 (2H, t, 77.1Hz), 4.10-4.16 (IH, m), 4.18 (IH, d, / 8.0Hz), 4.94 (IH, q, /

6.6Hz), 6.99-7.01 (2H, s), 7.16 (2H, s), 7.21-7.25 (3H, m), 7.65 (IH, s); m z (ES+)

572 (M⁺+H, 100%).

EXAMPLE 12 8-[(2R,3R,4R)-2-[(lR)-l-(3,5-Bis(trifluoromethyl)phenyl)ethoxy]-3-phenyl- tetrahydropyran-4-yl]methyl-2,2-dimethyl-l-oxa-8-aza-spiro[4.5]decane

A mixture of (2R,3R,4R)-2-[(lR)-l-(3,5-bis(trifluoromethyl)ρhenyl)ethoxy]-3- phenyl(tetrahydropyran)-4-aldehyde (see Example 45 in WO 00/56727) and 2,2-dimethyl-l-oxa-8-aza-spiro[4.5]decane (Description 18) was reacted and purified according to the procedure described in Example 6 to give the title compound. 1H NMR (360 MHz, CDCI₃): δ 1.16 (6H, s), 1.35 (3H, d, /6.6Hz), 1.38-1.61 (5H, m), 1.71 (4H, br s), 1.86-2.02 (5H, m), 2.15-2.22 (2H, m), 2.31-2.48 (2H, m), 3.50 (IH, br t), 4.08-4.15 (IH, m), 4.17 (IH, d, / 8.3Hz), 4.93 (IH, q, 76.6Hz), 6.98-7.00 (2H, m), 7.16 (2H, s) 7.20 (3H, br s), 7.65 (IH, s); m/z (ES+) 600 (M⁺+H, 100%).

EXAMPLE 13 8-[(2i?,3#,45!)-2-[(l^)-l-(3,5-Bis(trifluoromethyl)phenyl)ethoxy]-3-phenyl- tetrahydropyran-4-yl]methyl-l-oxa-8-aza-spiro[4.5]decane A mixture of (2R,3R,4R)-2-[(lR)-l-(3,5-bis(trifluoromethyl)phenyl)ethoxy]-3- phenyl(tetrahydropyran)-4-aldehyde (see Example 45 in WO 00/56727) and l-oxa-8- aza-spiro[4.5]decane (Description 17) was reacted and purified according to the procedure described in Example 6 to give the title compound. 1H NMR (360 MHz, CDC1₃): δ 1.35 (3H, d, 76.6Hz), 1.46-1.66 (8H, m), 1.84 (2H, qn), 1.90-2.32 (6H, m), 2.37 (2H, t, /7.1Hz), 3.53 (IH, br t), 3.73 (2H, t, /7.1Hz), 4.13 (IH, dd, / 11.7, 3.4Hz), 4.18 (IH, d, 8.2Hz), 4.94 (IH, q, 76.6Hz), 6.96-7.01 (2H, m), 7.16 (2H, s), 7.21 (3H, br s), 7.65 (IH, s); m/z (ES+) 572 (M*+H, 100%)

EXAMPLE 14 §-[(2^,3«,4^)-2-[(l^)-l-(3,5-Bis(trifluoromethyl)phenyl)ethoxy]-3-phenyl- tetrahydropyran-4-yl]methyl-l,l-diιnethyl-2-o-sa-8-asa-§piro[4.5] ecane A mixture of (2R,3R,4R)-2-[(lR)-l-(3,5-bis(trifluoromethyl)phenyl)ethoxy]-3- phenyl(tetrahydropyran)-4-aldehyde (see Example 45 in WO 00/56727) and l,l-dimethyl-2-oxa-8-aza-spiro[4.5]decane {Bioorg. and Med. Chem. Lett. 2002, 12, 1759) was reacted and purified according to the procedure described in Example 6 to give the title product.

1H NMR (360 MHz, CDC1₃): δ 1.03 (6H, s), 1.15-1.28 (2H, m), 1.35 (3H, d, /6.6Hz), 1.38-1.49 (2H, m), 1.50-1.59 (3H, m), 1.74 (2H, t, /7.1Hz), 1.86-2.05 (4H, m), 2.38 (IH, br t), 2.51-2.58 (IH, m), 2.61-2.68 (IH, m), 3.52 (IH, br t), 3.73 (2H, t, /7.1Hz), 4.13-4.19 (2H, m), 4.93 (IH, q, /6.6Hz), 6.98-7.01 (2H, m), 7.16 (2H, s) 7.22 (3H, br s), 7.65 (IH, s); m/z (ES+) 600 (M⁺+H, 100%).

EXAMPLE 15 8-[(2R,3R,4R)-2-[(lR)-l-(3,5-Bis(trifluoromethyl)phenyl)ethoxy]-3-(4- fluorophenyl)-tetrahydropyran-4-yl]methyl-2-oxa-8-aza-spiro[4.5]decan-4-one

Dess-Martin periodinane (0.6g, 1.4mmol) was added to a solution of (45)-8- [(2R,3R,4R)-2-[(lR)-l-(3,5-bis(trifluoromethyl)phenyl)ethoxy]-3-(4-fluoroρhenyl)- tetrahydropyran-4-yl]methyl-2-oxa-8-aza-spiro[4.5]decan-4-ol (Example 3 ; 0.85g,1.4mmol) in dichloromethane (20ml). The mixture was stirred at room temperature for 4 hours then quenched by addition of saturated sodium hydrogen carbonate. After 30 minutes the mixture was partitioned between water and dichloromethane. The organic extracts were dried and concentrated. The residue was chromatographed on silica gel eluting with 2% methanol / dichloromethane to give the title compound (510mg, 60%).

1H NMR (400 MHz, CDC1₃): δ 7.67 (IH, s), 7.17 (2H, s), 6.99-6.91 (4H, m), 4.96 (IH, q, 76.5Hz), 4.15-4.10 (2H, m), 3.96-3.90 (4H, m), 3.50 (IH, dt, 72, 12.1Hz), 2.64-2.62 (IH, m), 2.52-2.49 (IH, m), 2.38 (IH, dd, / 8.4, 10.9Hz), 2.02-1.87 (5H, m), 1.76-1.66 (3H, m), 1.45-1.39 (3H, m), 1.37 (3H, d, /6.6Hz).

EXAMPLE 16 (4^S)-8-[(2S₅3«₉4«)-2-[(l^)-l-(3_s5-Bis(trifluorometϊιyl)phenyI)ethoxy]-3-(4- fluorophenyϊ)-tetrahydropyran-4-yl]methyI-2-oxa-4-methyl-8-asa- spiro[4.5]decan-4-ol

Methyl magnesium chloride (3.0M solution in tetrahydrofuran, 90μl) was added to a solution of 8-[(2R,3R,4R)-2-[(lR)-l-(3,5-bis(trifluoromethyl)ρhenyl)ethoxy]-3-(4- fluorophenyl)tetrahydropyran-4-yl]methyl-2-oxa-8-aza-spiro[4.5]decan-4-one (Example 15; lOOmg, 0.16mmol) stirring in tetrahydrofuran (2ml) at room temperature. After 2 hours the reaction was quenched by addition of saturated ammonium chloride. The mixture was partitioned between ethyl acetate and water. The organic extracts were separated, dried and concentrated. The residue was chromatographed on silica gel eluting with dichloromethane/ methanol ammonia mixtures to give the title compound (66mg, 65%).

1H NMR (400 MHz, CDC1₃): δ 7.67 (IH, s), 7.17 (2H, s), 7.03-6.89 (4H, m), 4.95 (IH, q, / 6.6Hz), 4.13 (2H, m), 3.82 (IH, dd, 75.9, 8.5Hz), 3.68-3.61 (3H, m), 3.54- 3.51 (IH, m), 2.69-2.53 (2H, m), 2.39 (IH, td, 2.9, 10.9Hz), 2.01- 1.88 (5H, m), 1.62-1.41 (5H, m), 1.37 (3H, d, 76.6Hz), 1.36-1.35 (IH, m), 1.15 (3H, s); m/z (ES+) 620 (M+H)⁺.

EXAMPLE 17

(4R5)-8-[(2R,3R,4R)-2-[(lR)-l-(3,5-Bis(trifluoromethyl)phenyl)ethoxy]-(4- fluorophenyl)tetrahydropyran-4-yl]methyl-2-oxa-4-ethynyl-8-aza- spiro[4.5]decan-4-ol

Ethynyl magnesium bromide (0.5M solution in tetrahydrofuran, 0.5mL) was added to a solution of 8-[(2R,3R,4R)-2-[(lR)-l-(3,5-bis(trifluoromethyl)phenyl)ethoxy]-3-(4- fluorophenyl)-tetrahydropyran-4-yl]methyl-2-oxa-8-aza-spiro[4.5]decan-4-one (Example 15) (lOOmg, 0.16mmol) stirring in tetrahydrofuran (2ml) at room temp. After 2 hours the reaction was quenched by addition of saturated ammonium chloride. The mixture was partitioned between ethyl acetate and water. The organic extracts were separated, dried and concentrated. The residue was purified by chromatography on silica gel eluting with dichloromethane/ methanol/ ammonia mixtures to give the title compound (51mg, 49%).

1H NMR (400 MHz, CDC1₃): δ 7.67 (IH, s), 7.26 (2H, s), 7.17-6.90 (4H, m), 4.95 (IH, q, 76.6Hz), 4,15-4.10 (2H, m)₃ 3.97-3.95 (IH, m), 3.90-3.84 (2H, m), 3.62-3.48 (2H, m), 2.69-2.57 (3H, m), 2.45 -2.36 (IH, m), 2.08-1.39 (11H, m), 1.37 (3H, d, 7 6.6Hz); m/z (ES+) 630 (M+H)⁺.

EXAMPLE 18 (8S)-2-[((2«,31₉4«)-2-[(lfi)-l-[3,5-Bis(trifluoromethyl)phenyl]ethøxy]-3-phenyl)- tetrahydro-2£f-pyran-4-yl)methyl]-6-oxa-2-azaspiro[3.4]octan-8-ol (2R,3R,4R)-2-[(lR)-l-[3,5-Bis(trifluoromethyl)phenyl]ethoxy]-3-phenyltetrahydro- 2H-ρyran-4-carbaldehyde (WO 00/56727) (760mg, 1.7mmol) and 6-oxa-2- azaspiro[3.4]octan-8-ol (Description 19) (214mg, 1.7mmol) were dissolved in 1,2- dichloroethane (20mL) and sodium triacetoxyborohydride (636mg, 3mmol) was added. The suspension formed was stirred at ambient temperature for 16hrs then quenched with IN sodium hydroxide solution (50mL). The biphasic mixture formed was extracted with dichloromethane (2x50mL), and the extracts were dried (MgSO₄) then concentrated to give a gum. The residue was purified by silica chromatography to give a mixture of the two diastereomers, which could be separated by chiral ΗPLC (Cyclobond π, 7.5% EtOΗ/hexane) to give isomer A (for isomer B, see Example 19): Isomer A: 1H NMR (400 MHz, CDC1₃): δ 7.65 (IH, s), 7.24-7.21 (3H, m), 7.15 (2H, s), 7.03-6.99 (2H, m), 4.93 (IH, q, 76.6Hz), 4.21 (IH, dd, / 2.9, 4.6Hz), 4.17 (IH, d, / 8.4Hz), 4.12 (IH, ddd, / 1.7, 4.6, 11.7Hz) 3.84 (IH, dd, / 4.6, 9.9Hz), 3.76 (2H, s), 3.62 (IH, dd, / 2.8, 9.0Hz), 3.50 (IH, dt, / 2.4, 12.3Hz), 3.37 (IH, d, / 7.4Hz), 3.03 (IH, d, / 6.9Hz), 2.80 (2H, dd, / 7.4, 14.8Hz), 2.41 (IH, dd / 8.0, 11.3Hz), 2.16 (IH, dd, / 9.5, 12.3Hz), 2.07 (IH, dd, / 3.3, 11.8Hz) 1.85-1.75 (2H, m), 1.63 (IH, br m), 1.52-1.41 (IH, m), 1.35 (3H, d, /6.6Hz); m/z (ES⁺) 560 (M+H).

EXAMPLE 19 (8«)-2-[((2ie₅3i?_s4fi)-2-[(li?)-l-[3,5-bis(trifluoromethyϊ)phenyl]ethøxy]-3- pϊιenyltetrahydro-2fl^'-pyran-4-yl)methyl]-6-oxa-2-asa§piro[3.4]octan-8-øl This product was the second peak to elute from the prep HPLC run described in Example 18. Isomer B: 1H NMR (360 MHz, CDC1₃): δ 7.65 (IH, s), 7.27-7.12 (5H, m), 7.01-6.99 (2H, m), 4.93 (IH, q, 7 6.6Hz), 4.23 (IH, dd, 7 3.2, 5.3Hz), 4.17 (IH, dd, / 9.3Hz), 4.12 (IH, ddd, / 2.0, 7.3, 11.8Hz), 3.84 (IH, dd, 74.6, 9.8Hz), 3.75 (2H, s), 3.61 (IH, dd, J 3.1, 9.8Hz), 3.50 (IH, dt, 7 2.4, 12.3Hz), 3.42 (IH, d, 7 7.3Hz), 3.04 (IH, d, 7 6.8Hz), 2.87 (IH, d, 76.8Hz), 2.72 (IH, d, 76.8Hz), 2.41 (IH, dd, 7 8.5, 11Hz), 2.17 (IH, dd, 7 9.2, 12.0Hz), 2.07 (IH, dd, / 3.6, 12.6Hz), 1.86-1.76 (3H, m), 1.52-1.41 (IH, m), 1.35 (3H, d, / 6.6Hz); m/z (ES⁺) 560 (M+H).

EXAMPLE 20 (4«-?)-8-[(2R,31,4«)-2-[(li?)-l-(3,5-Bis(trifluoromethyl)phenyl)ethoxy]-3-phenyl- tetrahydropyran-4-yl]methyl-l-oxa-8-azaspiro[4.5]decan-4-ol This product was prepared according to the procedure described in Example 2 using (2R,3R,4R)-2-[(lR)-l-(3,5-bis(trifluoromethyl)phenyl)ethoxy]-3-phenyl- tetrahydropyran-4-carbaldehyde (WO 0056727) and 4-hydroxy-l-oxa- 8-azaspiro[4.5]decane (Description 20).

1H NMR (500 MHz, CDC1₃): δ 1.35 (3H, d, / 6.6Hz), 1.38-1.55 (2H, m), 1.6-1.98 (4H, m), 2.00-2.25 (4H, m), 2.38 (IH, dd, / 11.2, 8.4Hz), 2.39-2.7 (2H, m), 3.53 (IH, t, / 12.0Hz), 3.72-3.76 (IH, m), 3.89 (IH, multiplet centre (mc)), 4.13 (IH, dd, / 11.8, 3.4Hz), 4.20 (IH, d, / 8.3Hz), 4.94 (IH, q, / 6.5Hz), 6.99-7.00 (2H, m), 7.16 (2H, s), 7.21-7.22 (3H, m), 7.65 (IH, s); m/z (ES⁺) 588 (M⁺+1, 100%).

EXAMPLE 21 (4RS)-8-[(2R,3R,4R)-2-[(lR)-l-(3,5-Bis(trifluoromethyl)phenyl)ethoxy]-3-(4- fluorophenyl)-tetrahydropyran-4-yl]methyl-3,3-dimethyl-2-oxa-8- azaspiro[4.5]decan-4-ol

This product was prepared according to the procedure described in Example 6 using (2R,3R,4R)-2-[(lR)-l-(3,5-bis(trifluoromethyl)phenyl)ethoxy]-3-(4-fluorophenyl)- tetrahydropyran-4-carbaldehyde (Description 11) and 4-hydroxy-3,3-dimethyl- 2-oxa-8-azaspiro[4.5]decane (Description 21).

1H NMR (400 MHz, MeOD): δ 1.12 (3H, s), 1.24 (3H, s), 1.36 (3H, d, 76.6Hz), 1.39- 1.44 (2H, m), 1.56-1.78 (4H, m), 1.82-2.06 (4H, m), 2.37 (IH, t, 79.1Hz), 2.39-2.56 (2H, m), 3.44 (IH, s), 3.45-3.58 (3H, m), 4.10-4.14 (2H, m), 4.95 (IH, q, 7 6.6Hz), 6.91-6.99 (4H, m), 7.17 (IH, s), 7.67 (IH, s); m/z (ES⁺) 634 (M⁺+1, 100%). - Ill -

EXAMPLE 22

(4^*)-8-[(2«,3«_s4fi)-2-[(l«)-l-(3,5-Bis(trifluoromethyl)phenyl)ethoxy]-3-(4- fluorophenyl)-tetrahydropyran-4-yl]methyl-l-oxa-8-a2.aspiro[4.S]d©can-4-ol This compound was prepared from the reaction of the compounds of Description 11 and 20 following the procedure described in Example 6. The product was purified by chromatography on silica to give a mixture of two diastereomers, which were separated by chiral HPLC (Cyclobond π, 7.5% EtOH/hexane) to give isomer A: 1H NMR (500MHz, CDC1₃): δ 7.67 (IH, s), 7.17 (2H, s), 6.98-6.90 (4H, m), 4.95 (IH, q, 76.4Hz), 4.12 (2H, dd, 72.1, 8.3Hz), 3.93-3.89 (2H, m), 3.78-3.74 (IH, m), 3.52 (IH, dd, 7 12.1, 12.1Hz), 2.38 (2H, t, / 8.7Hz), 2.29-2.19 (3H, m), 2.01 (2H, m), 1.93 (2H, m), 1.85 (IH, m), 1.71 (IH, m), 1.41 (3H, s), 1.36 (3H, d, / 6.6Hz); m/z (ES+) 606.

EXAMPLE 23 (45*)-8-[(2R,3R,4R)-2-[(lR)-l-(3,5-Bis(trifluoromethyl)phenyl)ethoxy]-3-(4- fluorophenyl)-tetrahydropyran-4-yl]methyl-l-oxa-8-azaspiro[4.5]decan-4-ol

This compound was prepared from the reaction of the compounds of Description 11 and 20 following the procedure described in Example 6. The product was purified by chromatography on silica to give a mixture of two isomers, which were separated by chiral HPLC (Chiralpak AD, 2% EtOH in hexane) to give isomer A (see Example 22) and isomer B:

1H NMR (500MHz, CDC1₃): δ 7.67 (IH, s), 7.17 (2H, s), 6.98-6.90 (4H, m), 4.97-4.93 (IH, q, 76.6Hz), 4.12 (2H, dd, 72.6, 8.4Hz), 3.93-3.87 (2H, m), 3.79-3.73 (IH, m), 3.51 (IH, dd, / 10, 10Hz), 2.47 (IH, m), 2.38 (IH, t, / 9.6Hz), 2.26-2.19 (3H, m), 2.03-1.93 (5H, m), 1.88-1.82 (IH, m), 1.40-1.34 (6H, m); m/z (ES+) 606.

EXAMPLE 24 (4i?)-8-[((2i?₅3^,4«)-2-{(l^)-l-[3,5-Bis(trffluorømethyl)pbenyl]ethøxy}-3- phenyltetrahydro-2fl^'-pyran-4-yl)methyl]-4-methoxy-2-oxa-8-a2aspiro[4.5]decane To a solution of (4R)-8-[((2R,3R,4R)-2-{(lR)-l-[3,5- bis(trifluoromethyl)phenyl]ethoxy}-3-phenyltetrahydro-2H-pyran-4-yl]methyl-2-oxa- 8-azaspiro[4.5]decan-4-ol (Example 2) (100 mg, 0.17mmol) in anhydrous DMF (lmL) was added a 60 % dispersion of sodium hydride in mineral oil (40mg) under ice cooling. After 15 min of stirring at 0°C methyl iodide (31mg) was added and the mixture was stirred at r.t. for 18h, before it was diluted with water and extracted with ethyl acetate. The combined organic phases were washed with brine, dried over magnesium sulfate, filtered and concentrated in vacuo to give the crude product as colourless oil, which was then purified by flash chromatography on silica using 3-5 % methanol in dichloromethane as eluant. The desired product was obtained as colourless viscous oil (76 %).

1H NMR (360 MHz, CDC1₃): δ 7.65 (IH, s), 7.22 (3H, m), 7.16 (2H, s), 7.01 (2H, m), 4.94 (IH, q, 76.6Hz), 4.18 (IH, d, 78.4Hz), 4.13 (IH, dd, 73.3, 15Hz), 3.90 (IH, dd, 4.4, 9.8Hz), 3.77 (IH, dd, 72.0, 9.9Hz), 3.56-3.48 (3H, m), 3.38-3.34 (IH, m), 3.25 (3H, s), 2.41-2.35 (2H, m), 2.19-2.06 (2H, m), 2.06-1.87 (7H, m), 1.79-1.73 (IH, m), 1.47-1.39 (4H, m), 1.35 (3H, d, 76.6Hz); m z (ES+) 602.

EXAMPLE 25 (4R)-8-[((2R,3R,4R)-2-{(lR)-l-[3,5-Bis(trifluoromethyl)phenyl]ethoxy}-3-(4- fluorophenyl)tetrahydro-2-H-pyran-4-yl)methyl]-4-methoxy-2-oxa-8- azaspiro[4.5]decane

This compound was prepared from the compound of Example 4 following the procedure described in Example 24.

1H NMR (360 MHz, CDC1₃): δ 7.67 (IH, s), 7.17 (2H, s), 7.00-6.92 (4H, m), 4.95 (IH, q, /6.6Hz), 4.16-4.09 (2H, m), 3.90 (IH, dd, 74.4, 9.9Hz), 3.77 (IH, dd, 72.1, 9.9Hz), 3.56-3.48 (3H, m), 3.37 (IH, dd, 72.1, 4.3Hz), 3.26 (3H, s), 2.41-2.33 (2H, m), 2.16-2.07 (2H, m), 2.02-1.84 (5H, m), 1.79-1.71 (IH, m), 1.49-1.39 (2H, m), 1.36 (3H, d, 76.6Hz); m/z (ES+) 620, 362.

EXAMPLE 26 8-[((2S_s3«,4 )-2-{(M)-l-[3₅5-Bis(trifluoromethyl)phenyl]ethoxy}-3- phenyltetrahydro-2fi^r-pyran-4-yl)methyl]-l-oxa-8-a-.aspiro[4.5]decan-3-ol To a solution of (2R,3R,4R)-2-{(lR)-l-[3,5-bis(trifluoromethyl)phenyl]ethoxy}-3- ρhenyltetrahydro-2H-pyran-4-carbaldehyde (WO 00/56727) (0.97g, 2.71mmol) and triethylamine (0.72mL, 5.17mmol) in 1,2-dichloroethane (15mL) was added l-oxa-8- aza[4.5]decan-3-ol trifluoroacetate (Description 22) (775 mg), followed by sodium triacetoxyborohydride (630 mg, 3mmol). The reaction mixture was stirred for 2h at room temperature, then treated with dilute sodium hydroxide solution and extracted with dichloromethane. The combined organic phases were washed with brine, dried over magnesium sulfate, filtered and concentrated to give the crude product as a light brown oil. Flash chromatography on a column of silica gel with a gradient solvent system of 5-10 % methanol in dichloromethane afforded the title compound as a mixture of epimers (53 %, 0.85g).

1H NMR (360 MHz, CDC1₃): δ 7.66 (IH, s), 7.22 (3H, s), 7.16 (2H, s), 7.00 (2H, m), 4.94 (IH, q, / 6.6Hz), 4.43 (IH, br s), 4.20 (IH, d, 78.2Hz), 4.13 (IH, dd, 73.3, 11.9Hz), 3.82 (IH, dd, 74.2, 9.9Hz), 3.72 (IH, d, 710. IHz), 3.54 (IH, dd, / 12, 12Hz), 2.59-1.41 (ca. 20H, methylene envelope), 1.24 (3H, d, / 6.6Hz); m/z (ES+) 588.

EXAMPLE 27 8-[((2R,3R,4R)-2-{(lR)-l-[3,5-Bis(trifluoromethyl)phenyl]ethoxy}-3- phenyltetrahydro-2/7-pyran-4-yl)methyl]-l-oxa-8-azaspiro[4.5]decan-3-one

This compound was prepared from the compound of Example 26 following the procedure described in Example 15.

1H NMR (360 MHz, CDC1₃): δ 7.65 (IH, s), 7.23-7.19 (3H, m), 7.17 (2H, s), 7.09- 6.97 (2H, m), 4.94 (IH, q, / 6.5Hz), 4.18 (IH, d, / 8.3Hz), 4.13 (IH, dd, 74.2, 11.7Hz), 3.92 (2H, s), 3.53 (IH, t, 11. IHz), 2.49-2.42 (IH, m), 2.39 (IH, dd, 78.4, 10.8Hz), 2.31-2.17 (2H, m), 2.05 (2H, dd, / 10.2, 12.6Hz), 2.00-1.93 (3H, m), 1.76- 1.68 (2H, m), 1.67-1.58 (2H, m), 1.48-1.38 (IH, m), 1.36 (3H, d, /6.6Hz); m/z (ES+) 586.

EXAMPLE 28 {2R or S,5R or ,S)-5-((2R,3R,4R)-{(lR)-l-[3,5-Bis(trifluoromethyl)phenyl]- ethosy}tetrahydro-3-phenyl-2H-pyran-4-yl)tetrahydro-N-(phenylmethyl)-2- furanmethanan ine

A solution of {2RS,5R or 5)-5-((2R,3R,4R)-{(lR)-l-[3,5-bis(trifluoromethyl)phenyl]- ethoxy}tetrahydro-3-phenyl-2H-pyran-4-yl)-l,2,5-pentanetriol l-(4- methylbenzenesulfonate) 5-methanesulfonate (3:1 mixture of alcohol diastereoisomers; Description 27; 0.48 g, 0.63 mmol) and benzenemethanamine (0.69 mL, 6.3 mmol) in methanol (10 mL) was placed in a sealed tube and heated in a microwave oven at 140 °C for 10 minutes. The mixture was cooled and the solvent was evaporated under reduced pressure. The residue was dissolved in ethyl acetate (20 mL), washed with saturated aqueous potassium carbonate (20 mL) and brine (20 mL), dried (Na SO ), and the solvent was evaporated under reduced pressure. The residue was purified by preparative thin layer chromatography on silica gel, eluting with EtOAc/MeOH/NH₃(Aq.) (98:2:0.2), to give the title compound (single diastereoisomer) as a colorless oil (0.12 g, 31%).

1H NMR (500MHz, CD₃OD) δ 7.71 (IH, s), 7.31-7.06 (12H, m), 4.99 (IH, q, 7 6.5Hz), 4.34 (IH, d, 78.5Hz), 4.10 (IH, m), 3.94 (IH, m), 3.63 (3H, m), 3.53 (IH, m), 2.41 (3H, m), 2.29 (IH, m), 1.92 (IH, m), 1.71 (3H, m), 1.51 (IH, m), 1.36 (IH, m), 1.32 (3H, d, 76.6Hz); m/z (ES⁺) 608 (M+l).

EXAMPLE 29

(2S or R,5R or 5)-5-((2R,3R,4R)-{(lR)-l-[3,5-Bis(trifluoromethyl)phenyl]- ethoxy}tetrahydro-3-phenyl-2/7-pyran-4-yl)tetrahydro-N-(phenylmethyl)-2- furanmethanamine

The title compound (single diastereoisomer) was prepared from {2RS,5R or S)-5- ((2R,3R,4R)-{(lR)-l-[3,5-bis(trifluoromethyl)phenyl]ethoxy}tetrahydro-3-phenyl-2H- pyran-4-yl)-l,2,5-pentanetriol l-(4-methylbenzenesulfonate) 5-methanesulfonate (1:3 mixture of alcohol diastereoisomers; Description 28) according to the method of Example 28.

1H NMR (500MHz, CD₃OD): δ 7.71 (IH, s), 7.31-7.06 (12H, m), 4.99 (IH, q, 7 6.5Hz), 4.34 (IH, d, 78.5Hz), 4.10 (IH, m), 3.78 (IH, m), 3.63 (2H, ABX), 3.60 (IH, dt, / 2.0, 11.8Hz), 3.45 (IH, m), 2.53 (IH, dd, / 3.7, 12.0Hz), 2.46 (IH, dd, / 8.4, 12.0Hz), 2.39 (IH, dd, / 8.3, 11.8Hz), 2.27 (IH, m), 1.80 (IH, m), 1.65 (3H, m), 1.42 (2H, m), 1.32 (3H, d, / 6.6Hz); m z (ES⁺) 608 (M+l). EXAMPLE 30 (2R or S,5R or 5)-5-((2R,3R,4R)-{(lR)-l-[3,5-Bis(trifluoromethyl)phenyl]- ethoxy}tetrahydro-3-phenyl-2ff-pyran-4-yl)tetrahydro-2-fιιranmethanamine

Dried palladium hydroxide on carbon (20 mg) was added to a solution of

(2R or S,5R or 5)-5-((2R,3R,4i?)-{(lR)-l-[3,5-bis(trifluoromethyl)phenyl]ethoxy}- tetrahydro-3-phenyl-2H-pyran-4-yl)tetrahydro-N-(ρhenylmethyl)-2- furanmethanamine (Example 28; 0.12 g, 0.20 mmol) in ethanol (3 mL) and the mixture was stirred under hydrogen (1 Atmosphere) for 12 hours. The mixture was filtered through Celite™ and the solvent was evaporated under reduced pressure. The residue was purified by preparative thin layer chromatography on silica gel, eluting with EtOAc/MeOH/NH₃(Aq.) (90:10:1) then by preparative HPLC (Supelsosil™

ABZ+PLUS 100x21.2 mm i.d.; gradient 0.1% TFA-H₂O/45%MeCN to 0.1%

TFA-H₂O/55%MeCN over 10 min; 20 mL/min; 210 nm; 400 μl injections of a

20 mg/mL solution in MeCN), to give the title compound (single diastereoisomer) as a colorless oil (0.30 mg, 29%).

1H NMR (500MHz, CD₃OD) δ 7.71 (IH, s), 7.31 (2H, m), 7.17 (3H, m), 7.09 (2H, m), 4.99 (IH, q, /6.5Hz), 4.36 (IH, d, / 8.1Hz), 4.14 (IH, m), 3.83 (IH, m), 3.63

(IH, dt, /2.3, ll.OHz), 3.57 (IH, m), 2.53 (IH, m), 2.43 (2H, m), 2.32 (IH, m), 1.92

(IH, m), 1.73 (3H, m), 1.53 (IH, m), 1.40 (IH, m), 1.32 (3H, d, / 6.6Hz); m/z (ES⁺)

518 (M+l), 260 (M+l-Ci₀H₈F₆O).

EXAMPLE 31 (2S or R,5R or S)-5-((2R,3R,4R)-{(lR)-l-[3,5-Bis(trifluoromethyl)phenyl]- ethoxy}tetrahydro-3-phenyl-2/7-pyran-4-yl)-N,N-diethyltetrahydro-2- furanmethanamine

The title compound was prepared from {2S or R,5R or 5)-5-((2R,3R,4R)-{(lR)-l-[3,5- bis(trifluoromethyl)phenyl]ethoxy}tetrahydro-3-phenyl-2H-pyran-4-yl)tetrahydro-N-

(phenylmethyl)-2-furanmethanamine (Example 29) according to the method of

Example 30.

1H NMR (500MHz, CD₃OD) δ 7.71 (IH, s), 7.31 (2H, s), 7.17 (3H, m), 7.09 (2H, m),

4.99 (IH, q, /6.5Hz), 4.36 (IH, d, / 8.1Hz), 4.14 (IH, m), 3.63 (IH, m), 3.45 (IH, dt,

/2.3, ll.OHz), 3.30 (IH, m), 2.70-2.53 (5H, m), 2.41 (IH, dd, / 8.1, 12.0Hz), 2.33 (1H, m), 1.88 (IH, m), 1.70 (3H, m), 1.53 (IH, m), 1.40 (IH, m), 1.32 (3H, d, / 6.6Hz), 1.02 (6H, t, /7.1Hz); m/z (ES⁺) 574 (M+l).

EXAMPLE 32 (3 ? or S)-3-((2fi,3i?,4S)-{(li?)-l-[3,5-Bi§(trifluoromethyl)pheJttyI]efhøxy}- tetrahydro-3-phenyl-2fl^r-pyran-4-yl)tIιiømorpho ne; and (3S or «)-3-((2^,3^₅4«)-{(lff)-l-[3,5-bis(trifluoromethyI)phenyl]ethøxy}- tetrahydrø-3-phenyl-2fl^'-pyran-4-yl)tϊιiomorpholiιιe

Methanesulfonyl chloride (0.12 mL, 0.17 g, 1.5 mmol) was added dropwise to a stirred, cooled (-10 °C) solution of (2R,3R,4R,αR or S)- -{ [(2- hydroxyethyl)thio] methyl } -2- { ( IR)- 1 - [3 ,5-bis(trifluoromethyl)phenyl] ethoxy } - tetrahydro-3-phenyl-2H-pyran-4-methanol and {2R,3R,4R, S or R)-α-{ [(2- hydroxyethyl)thio]methyl } -2- { ( IR)- 1 - [3 ,5-bis(trifluoromethyl)phenyι] ethoxy } - tetrahydro-3-phenyl-2H-pyran-4-methanol (Description 30, 1:1 mixture of diastereoisomers; 269 mg, 0.5 mmol) and triethylamine (0.28 mL, 0.20 g, 2 mmol) in dichloromethane (5 mL) and the mixture was stirred at -10 °C for 10 minutes. Water (20 mL) was added and the mixture was extracted with dichloromethane (3 x 20 mL). The combined organic fractions were washed with aqueous citric acid (10%, 2 x 20 mL), saturated aqueous sodium hydrogen carbonate/water (1:1, 2 x 20 mL) and brine (20 mL), dried (MgSO₄) and the solvent was evaporated under reduced pressure. The residue was dissolved in methanolic ammonia (7M, 5 mL), placed in a sealed tube and heated in a microwave oven at 60 °C for 10 minutes. The mixture was cooled and the solvent was evaporated under reduced pressure. The residue was purified by column chromatography on silica gel, eluting with CΗ₂Cl₂/MeOΗ/NΗ₃(aq.) (99:1:0.1 increasing to 96:4:0.4), to give the title compound (1:1 mixture of thiomorpholine epimers) as a colorless foam (81 mg, 31%). 1H NMR (500MHz, CD₃OD) δ 7.71, 7.70 (IH, each s), 7.32-7.12 (7H, m), 4.99 (IH, m), 4.39, 4.32 (IH, each d, / 8.4Hz), 4,08 (IH, m), 3.58 (IH, m), 3.13-2.36 (8H, m), 2.08 (IH, m), 1.82-1.55 (2H, m), and 1.32, 1.31 (3H, each d, / 6.6Hz); m/z (ES⁺) 520 (M+l) and 262 (M+1-C₁₀H₈F₆O). EXAMPLE 33 (2S)-2-((2R,3R,4R)-{(lR)-l-[3,5-Bis(trifluoromethyl)phenyl]ethoxy}-tetrahydro-3- phenyl-2Jϊ-pyran-4-yl)-l-methyla»etϊdine

(lR)-l-((2R,3R,4R)-{(lR)-l-[3,5-Bis(trifluoromethyl)phenyl]ethoxy}tetrahydro-3- phenyl-2H-pyran-4-yl)-l,3-propanediol dimethylsulfonate (Description 35; 0.25 g₃ 0.39 mmol) was dissolved in methylamine (2.0M in methanol, 4 mL) and heated at 140 °C for 10 minutes in a sealed tube in a Smith microwave reactor. The mixture was cooled and the solvent was evaporated under reduced pressure. Saturated aqueous potassium carbonate (50 mL) was added and the mixture was extracted with ethyl acetate (3 x 50 mL). The combined organic fractions were dried (MgSO₄) and the solvent was evaporated under reduced pressure. The residue was purified by preparative thin layer chromatography on silica gel, eluting with CH₂Cl₂/MeOH/NH₃(Aq.) (100:10:1), to give the title compound as a pale yellow solid (20 mg, 10%).

1H NMR (500MHz, CD₃OD) δ 1.31 (3H, d, / 6.6Hz), 1.58 (IH, app dq,), 1.75 (IH, m), 1.81 (IH, m), 2.00 (IH, m), 2.10 (IH, m), 2.30 (IH, dd, / 8.2, 11.6Hz), 2.59 (IH, q, / 9.4Hz), 2.80 (IH, m), 3.22 (IH, m), 3.64 (IH, dd, / 9.7, 12.0Hz), 4.14 (IH, m), 4.34 (IH, d, / 8.2Hz), 4.99 (IH, q, /6.6Hz), 7.07-7.09 (2H, m), 7.19-7.24 (3H, m), 7.31 (2H, s), 7.71 (IH, s); m z (ES⁺) 488 (M+l) and 230 (M+1-C₁₀H₈F₆O).

EXAMPLE 34 (2R)-2-((2R,3R,4R)-{(lR)-l-[3,5-Bis(trifluoromethyl)phenyl]ethoxy}-tetrahydro- 3-phenyl-2fl^r-pyran-4-yl)-l-methylazetidine

Prepared from (15)-l-((2R,3R,4R)-{ (lR)-l-[3,5-bis(trifluoromethyl)phenyl]- ethoxy}tetrahydro-3-phenyl-2H-pyran-4-yl)-l,3-propanediol dimethylsulfonate (Description 36) according to the method of Example 33.

1H NMR (500MHz, CD₃OD) δ 1.05 (IH, m), 1.14 (IH, m), 1.31 (3H, d, 76.6Hz), 1.42 (IH, m), 1.80 (IH, dd, 74, 2Hz), 2.12 (IH, m), 2.33 (3H, s), 2.36 (IH, m), 2.62 (IH, m), 2.93 (IH, m), 3.14 (IH, m), 3.63 (IH, dd, 712.1, 2.1Hz), 4.12 (IH, m), 4.42 (IH, d, 78.5Hz), 4.99 (IH, m), 7.05-7.06 (2H, m), 7.15-7.18 (3H, m), 7.32 (2H, app. s), 7.71 (IH, s); m/z (ES⁺) 488 (M+l) and 230 (M+1-C₁₀H₈F₆O). EXAMPLE 35 4-((2R,3R,4R)-{(lR)-l-[3,5-Bis(trifluoromethyl)phenyl]ethoxy}tetrahydro-3- phβnyl-2Jϊ-pyran-4-yl)piperidm-4-ol

3-((2R,3R,4R)-{(lR)-l-[3,5-Bis(trifluoromethyl)phenyl]ethoxy}tetrahydro-3-phenyl- 2H-pyran-4-yl)-l,3,5-propanelriol 1,5-dimethylsulfonate (Description 40; 0.13 g, 0.19 mmol) was dissolved in methanolic ammonia (7M, 2 mL) and heated at 130 °C for 20 minutes in a sealed tube in a Smith microwave reactor. The mixture was cooled and the solvent was evaporated under reduced pressure. The residue was dissolved in water (25 mL) and basified with saturated aqueous potassium carbonate. The mixture was extracted with dichloromethane (3 x 30 mL). The combined organic fractions were dried (MgSO₄) and the solvent was evaporated under reduced pressure. The residue was purified by column chromatography on silica gel, eluting with CH₂Cl₂/MeOH/NH₃(Aq.) (90:10:1), to give the title compound as a colorless oil (25 mg, 25%).

1H NMR (500MHz, CDC1₃) δ 7.65 (IH, s), 7.24-7.18 (3H, m), 7.16 (2H, s), 7.12 (2H, m), 4.90 (IH, q, / 6.6Hz), 4.25 (IH, d, /7.4Hz), 4.16 (IH, br d, / 11Hz), 3.54 (IH, br t, / 11Hz), 2.84-2.79 (4H, m), 2.72 (IH, s), 2.02 (IH, br t, / 11Hz), 1.8 (IH, br s), 1.78 (IH, br d, / 11Hz), 1.64 (IH, br q, / 11Hz), 1.56-1.51 (3H, m), 1.36 (3H, d, / 6.6Hz), 1.31 (IH, m), 1.2 (IH, br s); m/z (ES⁺) 518 (M+l) and 260 (M+1-C₁₀H₈F₆O).

EXAMPLE 36 4-((2R,3R,4R)-{(lR)-l-[3,5-Bis(trifluoromethyl)phenyl]ethoxy}tetrahydro-3- phenyl-2JJ-pyran-4-yl)-l-cyclopropylpiperidin-4-ol

Prepared from 3-((2R,3R,4R)-{ (lR)-l-[3,5-bis(trifluoromethyl)ρhenyl]ethoxy }- tetrahydro-3-phenyl-2H-pyran-4-yl)-l,3,5-propanetriol 1,5-dimethylsulfonate (Description 40) and cyclopropylamine according to the method of Example 33. 1H NMR (500MHz, CD₃OD) δ 7.69 (IH, s), 7.27 (2H, s), 7.16-7.10 (5H, m), 4.92 (IH, q, /6.6Hz), 4.25 (IH, d, / 8.1Hz), 4.10 (IH, br d, / 12Hz), 3.60 (IH, br t, / 12Hz), 2.80 (IH, dd, / 11.1, 8.1Hz), 2.65 (IH, m), 2.52-2.44 (2H, m), 2.34 (IH, m), 2.08 (IH, br t, 712Hz), 1.78 (IH, br d, 712Hz), 1.69 (IH, br q, 7 12Hz), 1.56 (IH, m), 1.45 (IH, m), 1.34 (IH, m), 1.32 (3H, d, 76.6Hz), 1.12 (2H, m), 0. 1 (2H, m), 0.30 (2H, m); m/z (ES⁺) 558 (M+l) and 300 (M+1-C₁₀H₈F₆O). EXAMPLE 37 4-((2R,3R,4R)-{(lR)-l-[3,5-Bis(trifluoromethyl)phenyl]ethoxy}tetrahydro-3- phenyl-2Iϊ-pyran-4-yl)-l-(l-methylβthyl)piperidin-4-ol Prepared from 3-((2R,3R,4R)-{ (li?)-l-[3,5-bis(trifluoromethyl)phenyl]ethoxy }- tetrahydro-3-phenyl-2/f-pyran-4-yl)-l,3,5-propanetriol 1,5-dimethylsulfonate (Description 40) and isopropylamine according to the method of Example 33. 1H NMR (500MHz, CD₃OD) δ 7.70 (IH, s), 7.27 (2H, s), 7.18-7.11 (5H, m), 4.93 (IH, q, 76.6Hz), 4.27 (IH, d, 78.1Hz), 4.11 (IH, br d, 7 12Hz), 3.61 (IH, br t, 7 12Hz), 2.86 (IH, m), 2.80 (IH, dd, 7 11.1, 8.1Hz), 2.76-2.55 (4H, m), 2.12 (IH, br t, 7 12Hz), 1.79 (IH, br d, 7 12Hz), 1.70 (IH, br q, 7 12Hz), 1.60 (IH, m), 1.47 (IH, m), 1.32 (3H, d, 76.6Hz), 1.24 (2H, m), 1.09 (3H, d, 76.5Hz), 1.08 (3H, d, 76.5Hz); m/z (ES⁺) 560 (M+l).

EXAMPLE 38 (αR or 5)-α-((2R,3R,4R)-{(lR)-l-[3,5-Bis(trifluoromethyl)phenyl]ethoxy}- tetrahydro-3-phenyl-2/7-pyran-4-yl)-l-piperidineethanol Hydrochloride

A mixture of (2R,3R,4R)-2-{(lR)-l-[3,5-bis(trifluoromethyl)phenyl]ethoxy}- tetrahydro-4-[(2R or S)-oxiranyl]-3-phenyl-2H-pyran (Description 41, Isomer A; 70 mg, 0.15 mmol) and piperidine (45 μL, 39 mg, 0.46 mmol) in methanol (5 mL) was placed in a sealed tube and heated in a microwave oven at 130 °C for 20 minutes. The mixture was cooled and the solvent was evaporated under reduced pressure. The residue was purified by column chromatography on silica gel, eluting with EtOAc then CΗ₂Cl₂/MeOΗ/NΗ₃(Aq.) (95:5:0.5), then by column chromatography on silica gel, eluting with CH₂Cl₂/MeOH (90:10) to give (oR or S)-a-((2R,3R,4R)-{(lR)-l-[3,5- bis(trifluoromethyl)phenyl]ethoxy}tetrahydro-3-phenyl-2H-pyran-4-yl)-l- piperidineethanol as a solid (28 mg, 34%). The solid was dissolved in diethyl ether and ethereal hydrogen chloride (IM, 0.2 mL) was added. The solid was collected and dried in vacuo to give the title compound as a colorless solid. 1H NMR (500MHz, CDC1₃) δ 11.0 (IH, s), 7.66 (IH, s), 7.24 (3H, m), 7.18 (2H, s), 7.03 (2H, m), 4.95 (IH, q, 76.6Hz), 4.24 (IH, d, J 8.5Hz), 4.17 (IH, br d, / 11Hz), 3.76 (IH, br d, / 11Hz), 3.50 (IH, br t, / 11Hz), 3.43 (IH, br d, / 11Hz), 3.31 (IH, br d, / 11Hz), 2.94 (IH, br t, / 11Hz), 2.87 (IH, dd, / 11.2, 8.5Hz), 2.55 (IH, br q, / 11Hz), 2.40-2.10 (4H, m), 1.95-1.05 (6H, m), 1.35 (3H, d, 76.6Hz), 1.32 (IH, m); m/z (ES⁺) 546 (M+l) and 288 (M+1-C₁₀H₈F₆O).

EXAMPLE 39 (αS or «)-«-((2 ₅3^,4H)-{(l^)-l-[3,5-Bi§(trifluoromeihyl)pbenyl]etlιoxy}- tetrahydro-3-phenyl-2£f-pyran-4-yl)-l-piperidineethanol Hydrochloride Prepared from (2R,3R,4R)-2-{ (lR)-l-[3,5-bis(trifluoromethyl)phenyl]ethoxy }- tetrahydro-4-[(25 or R)-oxiranyl]-3-phenyl-2H-pyran (Description 41, Isomer B) and piperidine according to the method of Example 38.

1H NMR (400MHz, CDC1₃) δ 11.18 (IH, s), 7.65 (IH, s), 7.25 (3H, m), 7.12 (2H, s), 7.05 (2H, m), 4.92 (IH, q, /6.6Hz), 4.20 (IH, d, / 8.1Hz), 4.18 (IH, br d, / 11Hz), 3.85 (IH, br d, / 11Hz), 3.55 (IH, br t, / 11Hz), 3.40(1H, br d, / 11Hz), 3.06 (IH, br d, / 11Hz), 2.76-2.58 (3H, m), 2.48 (IH, dd, / 12.2, 8.1Hz), 2.29-1.45 (11H, m), 1.35 (3H, d, /6.6Hz); m/z (ES⁺) 546 (M+l) and 288 (M+1-C₁₀H₈F₆O).

EXAMPLE 40 (αR or 5,2R,3R,4R)-2-{(lR)-l-[3,5-Bis(trifluoromethyl)phenyl]ethoxy}- tetrahydro-α-[(dimethylamino)methyl]-3-phenyl-2/7-pyran-4-methanol Hydrochloride

Prepared from (2R,3R,4R)-2-{(lR)-l-[3,5-bis(trifluoromethyl)phenyl]ethoxy}- tetrahydro-4-[(2R or 5^,)-oxiranyl]-3-phenyl-2H-pyran (Description 41, Isomer A) and dimethylamine according to the method of Example 38.

1H NMR (500MHz, CDC1₃) δ 11.3 (IH, br s), 7.66 (IH, s), 7.24-7.26 (3H, m), 7.19 (2H, s), 7.04-7.05 (2H, m), 4.96 (IH, q, /6.6Hz), 4.26 (IH, d, / 8.4Hz), 4.18 (IH, dd, / 11.7, 3.3Hz), 3.70 (IH, br d, / 9.7Hz), 3.52 (IH, br t, / 11.8Hz), 3.09 (IH, br t, / 12.1Hz), 2.86 (IH, dd, / 11.0, 8.6Hz), 2.64 (6H, br s), 2.43 (IH, br d, / 12.3Hz), 1.89 (IH, m), 1.77 (IH, m), 1.60 (IH, br d, / 12.8Hz), 1.36 (3H, d, /6.6Hz); m/z (ES⁺) 506 (M+l) and 248 (M+1-C₁₀H₈F₆O). EXAMPLE 41 1,1-DimethyIethyl (2R or 5)-2-[(R or S)-((2R,3R,4R)-{(lR)-l-[3,5-

Bis(trϊfluoromethyl)phenyI]ethoxy}tetrahydro-3-phenyI-2U-pyran-4- yl)hydrosymethyl]-l-pyrrolidinecarbosylate (Isomers A and B) .sec-Butyllithium (1.4M in hexanes, 1.6 mL, 2.24 mmol) was added slowly to a stirred, cooled (-78 °C) solution of 1,1-dimethylethyl 1-pyrrolidinecarboxylate (0.4 mL, 2.24 mmol) and NjNjN^N'-tetramethyl-l^-ethanediamine (0.3 mL, 2.02 mmol) in diethyl ether (4.5 mL). The mixture was stirred at -78 °C for 3 hours, then (2R,3R,4R)-2-{(lR)-l-[3,5-bis(trifluoromethyl)phenyl]ethoxy}tetrahydro-3-phenyl- 2H-pyran-4-carboxaldehyde (WO 00/56727A1; 0.5 g, 1.12 mmol) in diethyl ether (1 mL) was added dropwise. The mixture was stirred at -78 °C for 30 minutes, then warmed to room temperature. Water (2 mL) was added slowly, then ethyl acetate (10 mL). The layers were separated and the organic layer was washed with brine (5 mL). The combined aqueous layers were extracted with ethyl acetate (10 mL). The combined organic layers were dried (Na₂SO ) and the solvent was evaporated under reduced pressure. The residue was purified by column chromatography on silica gel, eluting with hexane/EtOAc (85:15 increasing to 75:25), to give: 1,1-dimethylethyl (2R or S)-2-f(R or S)-((2R,3R,4R)-{(lR)-l-[3,5- bis(trifluoromethyl)phenyl]etlιoxy}tetrahydro-3-phenyl-2H-pyran-4- yl)hydroxymethyl]- 1-pyrrolidinecarboxylate (Isomer A; Single diastereoisomer; Stereochemistry unassigned) as a colorless oil (0.25 g, 36%); 1H NMR (500MΗz, CDC1₃) δ 7.67 (IH, s), 7.21 (5H, m), 7.20 (2H, s), 7.03 (2H, m), 4.95 (IH, q, /6.5Hz), 4.28 (IH, d, / 8.3Hz), 4.20 (IH, m), 3.87 (IH, m), 3.53 (IH, dt, / 1.6, 10.8Hz), 3.33 (2H, m), 3.11 (IH, m), 2.98 (IH, dd, / 8.3, 11.3Hz), 2.92 (IH, m), 2.04 (IH, m), 1.75 (3H, m), 1.60 (IH, m), 1.44 (11H, m), 1.35 (3H, d, / 6.6Hz); and 1,1-dimethylethyl (2R or S)-2-[(R orS)-((2R,3R,4R)-{(lR)-l-[3,5- bis(trifluoromethyl)phenyl]ethoxy}tetrahydro-3-phenyl-2H-pyran-4- ylβvydroxymethylj- 1-pyrrolidinecarboxylate (Isomer B; Single diastereoisomer; Stereochemistry unassigned) as a colorless oil (0.26 g, 38%); 1H NMR (500MHz, CDCI3) δ 7.67 (IH, s), 7.21 (5H, m), 7.20 (2H, s), 7.03 (2H, m), 4.95 (IH, q, /6.5Hz), 4.28 (IH, d, /8.3Hz), 4.20 (IH, m), 4.00-2.81 (5H, m), 1.93 (2H, m), 1.70 (3H, m), 1.44 (11H, m), 1.35 (3H, d, /6.6Hz). EXAMPLE 42

(IR or S, 7aR or S)-l-((2R,3R,4R)-{(lR)-l-[3,5-Bis(trifluoromethyl)phenyl]- ethoxy}tetrahydro-3-phenyl-2fl^'-pyran-4-yl)tetrahydro-lfl^',3fl^r- pyrrolo[l,2-c]oxa_3ol-3-one (Isomer A)

Sodium hydride (60% dispersion in mineral oil, 15 mg, 0.36 mmol) was added to a solution of 1,1-dimethylethyl (2R or S)-2-[{R or 5)-((2R,3R,4R)-{(lR)-l-[3,5- bis(trifluoromethyl)phenyl]ethoxy}tetrahydro-3-phenyl-2H-pyran-4- yl)hydroxymethyl]-l-pyrrolidinecarboxylate (Example 41, Isomer A; 75 mg, 0.12 mmol) in tetrahydrofuran (2 mL) and the mixture was stirred at room temperature for 15 hours. Water (2 mL) was added slowly, then ethyl acetate (10 mL). The layers were separated and the organic layer was washed with brine (5 mL). The combined aqueous layers were extracted with ethyl acetate (10 mL). The combined organic layers were dried (Na₂SO ) and the solvent was evaporated under reduced pressure. The residue was purified by column chromatography on silica gel, eluting with hexane/EtOAc (80:20 increasing to 60:40), to give the title compound (Single diastereoisomer; Stereochemistry unassigned) as a colorless foam (54 mg, 82%).

1H NMR (500MHz, CDC1₃) δ 7.67 (IH, s), 7.26 (3H, m), 7.20 (2H, s), 7.06 (2H, m), 4.95 (IH, q, / 6.5Hz), 4.28 (IH, d, / 8.3Hz), 4.20 (IH, dt, / 11.4, 3.4Hz), 3.96 (IH, t, / 3.5Hz), 3.53 (2H, m), 3.34 (IH, m), 3.06 (IH, m), 2.82 (IH, dd, / 8.3, 11.3Hz), 1.93 (2H, m), 1.78 (2H, m), 1.68 (2H, m), 1.35 (3H, d, / 6.6Hz), 1.20 (IH, m).

EXAMPLE 43

(IR or S, 7aR or 5)-l-((2R,3R,4R)-{(lR)-l-[3,5-Bis(trifluoromethyl)phenyl]- ethoxy}tetrahydro-3-phenyl-2H-pyran-4-yl)tetrahydro-lJ7,32ϊ- pyrrolo[l,2-c]oxazol-3-one (Isomer B)

Prepared from (2R or S)-2-[{R or ,S)-((2R,3R,4R)-{(lR)-l-[3,5- bis(trifluoromethyl)phenyl]ethoxy}tetrahydro-3-phenyl-2H-pyran-4- yl)hydroxymethyl]-l-pyrrolidinecarboxylate (Example 41, Isomer B) according to the method of Example 42.

1H NMR (500MHz, CDC1₃) δ 7.67 (IH, s), 7.30 (2H, s), 7.24 (3H, m), 7.08 (2H, m),

4.98 (IH, q, / 6.5Hz), 4.54 (IH, dd, / 6.5, 8.6Hz), 4.42 (IH, d, / 7.2Hz), 4.17 (IH, dt,

/ 11.9, 4.0Hz), 3.60 (IH, dt, / 1.6, 10.8Hz), 3.55 (IH, m), 2.95 (2H, m), 2.62 (IH, dd, 7.3, 9.5Hz), 2.14 (IH, m), 1.98 (IH, m), 1.86 (IH, m), 1.78 (IH, m), 1.52 (IH, m), 1.39 (3H, d, 76.6Hz), 1.18 (IH, m), 1.04 (IH, m).

EXAMPLE 44 {2R,3R,4R,®^- or S)-α-[(2R or S)-2-Pyrrolidinyl]-2-{(l^)-l-[3,5- bis(trifluoromethyl)phenyl]ethoxy}tetrahydro-3-phenyl-2fl^'-pyran-4-methanøl (Isomer B)

Lithium hydroxide (30 mg, 0.70 mmol) was added to a solution of (IR or S, 7aR or 5)- l-((2R,3R,4R)-{(lR)-l-[3,5-bis(trifluoromethyl)phenyl]ethoxy}-tetrahydro-3-phenyl- 2H-pyran-4-yl)tetrahydro-lH,3H-pyrrolo[l,2-c]oxazol-3-one (Example 43, Isomer B; 76 mg, 0.14 mmol) in tetrahydrofuran (1 mL), methanol (1 mL) and water (1 mL) and the mixture was stirred at 50 °C for 3 days. The mixture was cooled and water (5 mL) and ethyl acetate (10 mL) were added. The layers were separated and the organic layer was washed with brine (5 mL). The combined aqueous layers were extracted with ethyl acetate (10 mL). The combined organic layers were dried (Na₂SO₄) and the solvent was evaporated under reduced pressure. The residue was purified by preparative thin layer chromatography on silica gel, eluting with EtOAc/MeOΗ/NΗ₃(Aq.) (85:15:1.5), to give the title compound (Single diastereoisomer; Stereochemistry unassigned) as a colorless oil (50 mg, 70%). 1H NMR (500MHz, CD₃OD) δ 7.71 (IH, s), 7.32 (2H, s), 7.22-7.12 (5H, m), 5.00 (IH, q, /6.5Hz), 4.39 (IH, d, / 8.6Hz), 4.11 (IH, dt, 7 11.4, 3.4Hz), 3.64 (IH, dt, / 2.4, 12.0Hz), 2.98 (IH, dd, / 2.0, 8.2Hz), 2.90 (IH, q, /7.8Hz), 2.79 (2H, m), 2.70 (IH, m), 2.10 (IH, m), 1.83 (IH, m), 1.73 (IH, m), 1.63 (3H, m), 1.41 (IH, m), 1.33 (3H, d, /6.6Hz); m/z (ES⁺) 518 (M+l), 260 (M+l- oHgFβO).

Claims

1. A compound of the formula (I):

wherein

W represents:

R¹ is hydrogen, halogen, C_1-6alkyl, C_1-6alkoxy, fluoroC_1-6alkyl, fluoroC ealkoxy, C_3-7cycloalkyl, C_3-7cycloalkylC_1-4alkyl, NO₂, CN, SR^a, SOR^a, SO₂R^a, CO₂R^a, CONR^aR^b, C_2-6alkenyl, C_2-6alkynyl or C_1-4alkyl substituted by C_1- alkoxy, wherein R^a and R^b each independently represent hydrogen or C^alkyl;

R² is hydrogen, halogen, C_halky!, fluoroC_1-6alkyl or ^alkoxy substituted by C_1- alkoxy;

R³ is hydrogen, halogen or fluoro -ealkyl;

R⁴ is hydrogen, halogen, C βalkyl, C_1-6alkoxy, fluoro -ealkyl, fluoroC_1-6alkoxy, hydroxy, NO₂, CN, SR^a, SOR^a, SO₂R^a, CO₂R^a, CONR^aR^b, C _-6alkenyl, C_2-6alkynyl or C_1-4alkyl substituted by C_1-4alkoxy, wherein R and R^b are as previously defined;

R⁶ represents hydrogen or a C_1-4alkyl group optionally substituted by a hydroxy group;

R⁷ represents hydrogen, C_1-6alkyl, fluoroC_1-6alkyl, hydroxy, C_1-6alkoxy or hydroxyC_1-6alkyl;

R and R each independently represent hydrogen, halogen, C_1-6alkyl, CH₂OR^c, oxo, CO₂R^a or CONR^aR^b where R^a and R are as previously defined and R^c represents hydrogen, C_1-6alkyl or phenyl; and pharmaceutically acceptable salts thereof; wherein when W is

A represents an oxygen atom or a CH group;

B represents an oxygen atom or a CH₂ group, with the proviso that when A is an oxygen atom, B is a CH group, and when A is a CH₂ group, B is an oxygen atom; n is 1 or 2; m is 1, 2 or 3, with the proviso that the sum total of m + n is 2, 3 or 4; R¹⁰ is hydrogen, halogen, hydroxy, C_1-4alkyl, C_3-7cycloalkyl, C_3-7cycloalkylC_1-4alkyl, hydroxyC_1- alkyl, C_1-4alkoxyC_1-4alkyl, C_2-6alkenyl or C_2-6alkynyl;

R¹¹ is hydrogen, halogen, hydroxy or C_1-4alkyl; or R¹⁰ and R¹ may together represent an oxo (=O) group, with the proviso that the oxo group is not adjacent to the oxygen atom represented by either A or B; wherein one or both of R¹⁰ and R¹¹ may replace one or both hydrogen atoms in the CH₂ group represented by A or B; wherein when W is

R¹⁰ is hydrogen, C_1-4alkyl, C3_-7cycloalkyl, C3- cycloalkylC_1-4alkyl, C_2-4alkyl substituted by a C_1-4alkoxy or hydroxyl group;

R¹¹ is hydrogen or C_1-4alkyl, C_3- cycloalkyl, C_3- cycloalkylC_1-4alkyl, or C_1-4alkyl substituted by C_1-4alkoxy, hydroxyl or phenyl, wherein said phenyl group is optionally substituted by one, two or three substituents selected from C_1-6alkyl, . ₆alkoxy, halogen and trifluoromethyl; or R¹⁰, R¹¹ and the nitrogen atom to which they are attached form a heteroaliphatic ring of 4 to 7 ring atoms, optionally substituted by one or two groups selected from hydroxy, COR^e, CO₂R^e, C_1-4alkyl optionally substituted by a C_1-4alkoxy or hydroxyl group, or C_1-4alkoxy optionally substituted by a C_1-4alkoxy or hydroxyl group, which heteroaliphatic ring may optionally contain an oxygen or sulphur ring atom, a group S(O) or S(O)₂ or a second nitrogen atom which will be part of a NH or NR^d moiety, where R^d is C_1-4alkyl optionally substituted by hydroxy or C_1-4alkoxy, and where R^e is hydrogen, C_1-4alkyl or benzyl;

R¹² represents halogen, hydroxy, C_1-4alkoxy or fluoroC_1-4alkoxy, or where p is 2, then two R⁹ groups may together represent an oxo (=O) group; m is zero, 1 or 2; n is 1, 2 or 3, with the proviso that the sum total of m + n is 2 or 3; and p is zero, 1 or 2; wherein when W is

A represents NR¹² or S(O)_q;

R¹⁰ and R¹¹ each independently represent hydrogen, fluorine, COR^e, CO₂R^e, C_1-4alkyl optionally substituted by a C_1-4alkoxy or hydroxyl group, or C_1-4alkoxy optionally substituted by a C_1-4alkoxy or hydroxyl group, where R^e is hydrogen, methyl ethyl or benzyl; or, when they are attached to the same carbon atom, R¹⁰ and R¹¹ may together represent =O, =CHCO R\ -O(CH₂)_mO-, -CH₂O(CH₂)_p-, -CH₂OCH₂C(O)-, -CH₂OCH₂CH(OH)-, -CH₂OCH₂C(CH₃)₂-, -CH₂OC(CH₃)₂CH₂-, -C(CH₃)₂OCH₂CH₂-, -CH₂C(O)OCH₂-, -OC(O)CH₂CH₂-, -C(O)OCH₂CH₂-, -C(O)OC(CH₃)₂CH₂-, -C(O)OCH₂C(CH₃)₂-, -OCH₂(CH₂)_p-, -OC(CH₃)₂CH₂CH₂-, -OCH₂C(CH₃)₂CH₂-, -OCH₂CH₂C(CH₃)₂-, -OCH₂CH=CHCH₂-, -OCH₂CH(OH)CH₂CH₂-, -OCH₂CH₂CH(OH)CH₂-, -OCH₂C(O)CH₂CH₂-, -OCH₂CH₂C(O)CH₂-, or a group of the formula

where R^a is as previously defined, or, where they are attached to adjacent carbon atoms, R¹⁰ and R^π may together represent -OCH₂CH₂- or -OCH₂CH(OH)-, or R¹⁰ and R¹¹ may together form a fused benzene ring; or, R¹⁰ and R¹¹ together form a C_1-2alkylene bridge across the pyrrolidine, piperidine, morpholine or piperazine ring to which they are attached; R¹² represents hydrogen, benzyl, C_1- alkyl, C_3-7cycloalkyl, C_3-7cycloalkylC_1- alkyl, COC_1-6alkyl, CO₂C_1-6alkyl or C_2- alkyl substituted by a C_1-4alkoxy or hydroxyl group;

R¹³ represents hydrogen, halogen, hydroxy, C_1- alkyl, hydroxyC_1-4alkyl or fluoroC_1- alkyl; m is 1 or 2; p is 1, 2 or 3; and q is zero, 1 or 2; wherein when W is

R , 10 represents halogen, hydroxy, C_1- alkoxy or fluoroC_1- alkoxy, or where p is 2, then two R¹⁰ groups may together represent an oxo (=O) group;

R¹¹ represents hydrogen, benzyl, C_1-4alkyl, C_3-7cycloalkyl, C_3-7cycloalkylC_1-4alkyl, or C_2-4alkyl substituted by a C_1-4alkoxy or hydroxyl group; m is zero, 1 or 2; n is zero or 1, with the proviso that the sum total of m + n is 1, 2 or 3, and with the further proviso that when m is 2 then n is 1; and p is zero, 1 or 2; wherein when W is

X represents -CHF-, -CF₂-, -CH(OH)- or -CH(OC_1-4alkyl)-; Y represents -NR¹³R¹⁴ or a group selected from:

Z represents O, S, SO, SO or NR¹²;

R¹⁰ represents hydrogen, halogen, hydroxy, C_1-4alkoxy or fluoroC_1- alkoxy;

R¹¹ represents hydrogen, halogen, hydroxy, C_1- alkoxy or fluoroC_1-4alkoxy; or R¹⁰ and R¹¹ together represent an oxo (=O) group;

R¹² represents C_1-6alkyl, COC_1-6alkyl, CO₂C_1-6alkyl;

R¹³ and R¹⁴ each independently represent hydrogen, C_1-6alkyl, C_3-7cycloalkyl or fluoroC_1-6alkyl, wherein said alkyl or cycloalkyl groups are optionally substituted with a group selected from hydroxy, C_1-4alkoxy, -NHCOC_1-4alkyl or -NHCO₂C_1-4alkyl; m is 1 or 2; n is 1 or 2; and p is 1 or 2; wherein when W is

X represents -CHR¹³-, -CF₂- or -C(=O)-;

R¹⁰ represents halogen, hydroxy, C_1-4alkoxy or fluoroC_1-4alkoxy, or where p is 2, then two R¹⁰ groups may together represent an oxo (=O) group;

R¹¹ represents hydrogen, benzyl, C_1-6alkyl, C_3-7cycloalkyl, C_3-7cycloalkylC_1-4alkyl, COC_1-6alkyl, CO₂C_1-6alkyl or C_2- alkyl substituted by a C_1- alkoxy or hydroxyl group;

R represents hydrogen, fluorine, hydroxy, C_1-4alkoxy, or R is linked to R

1 1 1 such that there is formed a ring, the linkage -R -R - being selected from: (a) -OC(O)-,

(b) -OS(O)₂-,

(c) — ZCH₂CH₂-,

(d) — CH CH CH - ,

(e) -ZCH₂C(O)-,

( ) -ZCH₂CH₂C(O ,

(g) -ZC(O)CH₂-,

(h) -ZC(O)CH₂CH₂-, and

(i) -ZCH₂C(O)CH₂-; wherein Z represents O, NH or N(C_1-6alkyl); m is 1 or 2; and p is zero, 1 or 2; or a pharmaceutically acceptable salt thereof.

2. A compound as claimed in Claim 1 of the formula (la)

wherein A, B, R , R , m and n are as hereinbefore defined as for when W is

or a pharmaceutically acceptable salt thereof.

3. A compound as claimed in Claim 2 of the formula (Iaa)

wherein

A¹ is fluorine or CF₃;

A² is fluorine or CF₃;

A is hydrogen or fluorine;

A⁴ is hydrogen, fluorine or bromine;

A is methyl or hydroxymethyl; and

A, B, R¹⁰ and R^π are as defined in Claim 2; or a pharmaceutically acceptable salt thereof.

A compound as claimed in Claim 2 of the formula (lab)

wherein

X represents CHOH or C=O;

R¹ is fluorine or trifluoromethyl;

R is fluorine or trifluoromethyl;

R is methyl or hydroxymethyl;

R⁴ is hydrogen or fluorine; and

R⁵ is hydrogen or fluorine; or a pharmaceutically acceptable salt thereof.

5. A compound as claimed in Claim 4 of the formula (laba)

wherein

A¹ is hydrogen or fluorine; and

A² is hydrogen or fluorine; or a pharmaceutically acceptable salt thereof.

6. A compound as claimed in Claim 1 selected from: (4RS')-8-[(2R,3R,4R)-2-[(lR)-l-(3,5-bis(trifluoromethyl)ρhenyl)ethoxy]-3-phenyl- tetrahydropyran-4-yl] methyl-2-oxa-8-aza-spiro[4.5] decan-4-ol ; (4S)-8-[(2R,3R,4R)-2-[(lR)-l-(3,5-bis(trifluoromethyl)phenyl)ethoxy]-3-(4- fluorophenyl)tetrahydropyran-4-yl]methyl-2-oxa-8-aza-spiro[4.5]decan-4-ol; (4R)-8-[(2R,3R,4R)-2-[(lR)-l-(3,5-bis(trifluoromethyl)phenyl)ethoxy]-3-(4- fluorophenyl)tetrahydropyran-4-yl]methyl-2-oxa-8-aza-spiro[4.5]decan-4-ol; (4RS)-8-[(2R,3R,4R)-2-[(lR)-l-(3,5-bis(trifluoromethyl)phenyl)ethoxy]-3-(3,4- difluorophenyl)tetrahydropyran-4-yl]methyl-2-oxa-8-aza-spiro[4.5]decan-4-ol; 8-[(2R,3R,4R)-2-[(lR)-l-(3,5-bis(trifluoromethyl)phenyl)ethoxy]-3-(4- fluorophenyl)tetraliydropyran-4-yl]methyl-2-oxa-8-aza-spiro[4.5]decane; 8-[(2R,35,45)-2-[(lR)-l-(3,5-bis(trifluoromethyl)phenyl)ethoxy]-3- phenyl(tetrahydropyran-4-yl)]methyl-l,l-dimethyl-2-oxa-8-aza-spiro[4.5]decane; 8-[(2R,3₁S,4₁S)-2-[(lR)-l-(3,5-bis(trifluoromethyl)phenyl)ethoxy]-3- phenyl(tetrahydropyran-4-yl)]methyl-2,2-dimethyl-l-oxa-8-aza-spiro[4.5]decane; 8-[(2R,35,45)-2-[( IR)- 1 -(3 ,5-bis(trifluoromethyl)phenyl)ethoxy]-3-phenyl- tetrahydropyran-4-yl]methyl-l-oxa-8-aza-spiro[4.5]decane; 8-[(2R,35,45)-2-[(lR)-l-(3,5-bis(trifluoromethyl)phenyl)ethoxy]-3-phenyl- tetrahydropyran-4-yl]methyl-2-oxa-8-aza-spiro[4.5]decane; 8-[(2R,3R,4R)-2-[(lR)-l-(3,5-bis(trifluoromethyl)phenyl)ethoxy]-3-phenyl- tetrahydropyran-4-yl]methyl-2-oxa-8-aza-spiro[4.5]decane; 8-[(2R,3R,4R)-2-[(lR)-l-(3,5-bis(trifluoromethyl)phenyl)ethoxy]-3-phenyl- tetrahydropyran-4-yl]methyl-2,2-dimethyl-l-oxa-8-aza-spiro[4.5]decane; 8-[(2R,3R,4R)-2-[(lR)-l-(3,5-bis(trifluoromethyl)phenyl)ethoxy]-3-phenyl- tetrahydropyran-4-yl]methyl-l-oxa-8-aza-spiro[4.5]decane; 8-[(2R,3R,4R)-2-[(lR)-l-(3,5-bis(trifluoromethyl)phenyl)ethoxy]-3-phenyl- tetrahydropyran-4-yl]methyl-l,l-dimethyl-2-oxa-8-aza-spiro[4.5]decane; 8-[(2R,3R,4R)-2-[(lR)-l-(3,5-bis(trifluoromethyl)ρhenyl)ethoxy]-3-(4- fluorophenyl)tetrahydropyran-4-yl]methyl-2-oxa-8-aza-spiro[4.5]decan-4-one; (4R_lS)-8-[(2R,3R,4R)-2-[(lR)-l-(3,5-bis(trifluoromethyl)phenyl)ethoxy]-3-(4- fluorophenyl)tetrahydropyran-4-yl]methyl-2-oxa-4-methyl-8-aza-spiro[4.5]decan-4- ol;

(4R)-8-[(2R,3R,4R)-2-[(lR)-l-(3,5-bis(trifluoromethyl)phenyl)ethoxy]-3-(3-bromo-4- fluorophenyl)tetrahydropyran-4-yl]methyl-2-oxa-8-aza-spiro[4.5]decan-4-ol; (4R5)-8-[(2R,3R,4R)-2-[(lR)-l-(3,5-bis(trifluoromethyl)phenyl)ethoxy]-(4- fluorophenyl)telrahydropyran-4-yl]methyl-2-oxa-4-ethynyl-8-aza-spiro[4.5]decan-4- ol; (85)-2-[((2R,3R,4R)-2-[(lR)-l-[3,5-bis(trifluoromethyl)phenyl]ethoxy]-3- phenyltetrahydro-2H-pyran-4-yl)methyl]-6-oxa-2-azaspiro[3.4]octan-8-ol;

(8R)-2-[((2R,3R,4R)-2-[(lR)-l-[3,5-bis(trifluoromethyl)phenyl]ethoxy]-3- phenyl)tetrahydro-2H-pyran-4-yl)methyl]-6-oxa-2-azaspiro[3.4]octan-8-ol;

(4R5)-8-[(2R,3R,4R)-2-[(lR)-l-(3,5-bis(trifluoromethyl)phenyl)ethoxy]-3- phenyltetrahydropyran-4-yl]methyl-l-oxa-8-azaspiro[4.5]decan-4-ol;

(4R_lS)-8-[(2R,3R,4R)-2-[(lR)-l-(3,5-bis(trifluoromethyl)ρhenyl)ethoxy]-3-(4- fluorophenyl)tetrahydropyran-4-yl]methyl-3,3-dimethyl-2-oxa-8-azaspiro[4.5]decan- 4-ol;

(4R*)-8-[(2R,3R,4R)-2-[(lR)-l-(3,5-bis(trifluoromethyl)ρhenyl)ethoxy]-3-(4- fluorophenyl)tetrahydropyran-4-yl]methyl-l-oxa-8-azaspiro[4.5]decan-4-ol;

(45*)-8-[(2R,3R,4R)-2-[(lR)-l-(3,5-bis(trifluoromethyl)phenyl)ethoxy]-3-(4- fluoroρhenyl)tetrahydropyran-4-yl] methyl- 1 -oxa-8-azaspiro [4.5] decan-4-ol ;

(4R)-8-[((2R,3R,4R)-2-{(lR)-l-[3,5-bis(trifluoromethyl)phenyl]ethoxy}-3- phenyltetrahydro-2H-pyran-4-yl)methyl]-4-methoxy-2-oxa-8-azaspiro[4.5]decane;

8-[((2R,3R,4R)-2-{(lR)-l-[3,5-bis(trifluoromethyl)phenyl]ethoxy}-3- phenyltetrahydro-2H-pyran-4-yl)methyl] - 1 -oxa-8-azaspiro [4.5] decan-3 -ol ; 8-[((2R,3R,4R)-2-{(lR)-l-[3,5-bis(trifluoromethyl)phenyl]ethoxy}-3- phenyltetrahydro-2H-pyran-4-yl)methyl]-l-oxa-8-azaspiro[4.5]decan-3-one;

(2R or S,5R or 5)-5-((2R,3R,4R)-{(lR)-l-[3,5-bis(trifluoromethyl)phenyl]ethoxy}- tetrahydro-3-phenyl-2H-pyran-4-yl)tetrahydro-N-(phenylmethyl)-2- furanmethanamine;

{2S or R,5R or 5)-5-((2R,3R,4R)-{(lR)-l-[3,5-bis(trifluoromethyl)phenyl]ethoxy}- tetrahydro-3-phenyl-2H-pyran-4-yl)tetrahydro-N-(phenylmethyl)-2- furanmethan amine;

(2R or <S,5R or 5)-5-((2R,3R,4R)-{(li?)-l-[3,5-bis(trifluoromethyl)phenyl]ethoxy}- tetrahydro-3-phenyl-2H-pyran-4-yl)tetrahydro-2-furanmethanamine; (25 or R,5R or 5)-5-((2R,3R,4R)-{(lR)-l-[3,5-bis(trifluoromethyl)phenyl]ethoxy}- tetrahydro-3-phenyl-2H-pyran-4-yl)-N,N-diethyltetrahydro-2-furanmethanamine; (3R or 5)-3-((2R,3R,4R)-{(l_JR)-l-[3,5-bis(trifluoromethyl)phenyl]ethoxy}tetrahydro- 3-phenyl-2H-pyran-4-yl)thiomorpholine; and

(35 or R)-3-((2R,3R,4R)-{(li?)-l-[3,5-bis(trifluoromethyl)phenyl]ethoxy}tetrahydro- 3-phenyl-2H-pyran-4-yl)thiomorpholine;

(25)-2-((2R,3R,4R)-{(lR)-l-[3,5-bis(trifluoromethyl)ρhenyl]ethoxy}tetrahydro-3- phenyl-2H-pyran-4-yl)-l-methylazetidine;

(2R)-2-((2R,3R,4R)-{(lR)-l-[3,5-bis(trifluoromethyl)ρhenyl]ethoxy}tetrahydro-3- phenyl-2H-pyran-4-yl)-l-mβthylazetidine;

4-((2R,3R,4R)-{(lR)-l-[3,5-bis(trifluoromethyl)phenyl]ethoxy}tetrahydro-3-phenyl- 2H-pyran-4-yl)piperidin-4-ol;

4-((2R,3R,4R)-{(lR)-l-[3,5-bis(trifluoromethyl)ρhenyl]ethoxy}tetrahydro-3-phenyl- 2H-pyran-4-yl)-l-cyclopropylpiperidin-4-ol; and

4-((2R,3R,4R)-{(lR)-l-[3,5-bis(trifluoromethyl)ρhenyl]ethoxy}tetrahydro-3-phenyl- 2H-pyran-4-yl)- 1 -( 1 -methylethyl)piperidin-4-ol ;

(αR or S)-α-((2R,3R,4R)- { ( IR)- 1 -[3 ,5-bis(trifluoromethyl)phenyl]ethoxy } tetrahydro- 3-phenyl-2H-pyran-4-yl)-l-piperidineethanol;

{aS or R)-α-((2R,3R,4R)-{ (lR)-l-[3,5-bis(trifluoromethyl)phenyl]ethoxy jtetrahydro- 3-phenyl-2H-pyran-4-yl)-l-piperidineethanol; and

(αR or 5,2R,3R,4R)-2-{ (lR)-l-[3,5-bis(trifluoromethyl)phenyl]ethoxy }tetrahydro-α- [(dimethylamino)methyl]-3-phenyl-2H-pyran-4-methanol; 1,1-dimethylethyl (2R or S)-2-[{R or 5)-((2R,3R,4R)-{(lR)-l-[3,5- bis(trifluoromethyl)phenyl]ethoxy}tetrahydro-3-phenyl-2H-pyran-4- yl)hydroxymethyl]-l-pyrrolidinecarboxylate;

(IR or S, 7aR or .S)-l-((2R,3R,4R)-{(lR)-l-[3,5-bis(trifluoromethyl)phenyl]- ethoxy } tetrahydro-3 -phenyl-2H-pyran-4-yl)tetrahydro- lH,3H-pyrrolo [ 1 ,2-c] oxazol-3- one; and

(2R,3R,4R,αR or 5)-α-[(2R or 5)-2-pyrrolidinyl]-2-{(lR)-l-[3,5- bis(trifluoromethyl)phenyl]ethoxy}tetrahydro-3-phenyl-2H-pyran-4-methanol; or a pharmaceutically acceptable salt thereof.

8. A compound as claimed in any one of Claims 1 to 7 for use in therapy. - oo -

9. A pharmaceutical composition comprising a compound as claimed in any one of Claims 1 to 7, together with at least one pharmaceutically acceptable carrier or excipient.

10. A method for the treatment or prevention of physiological disorders associated with an excess of tachykinins, which method comprises administration to a patient in need thereof of a tachykinin reducing amount of a compound according to Claim 1.

11. A method for the treatment or prevention of pain or inflammation, migraine, emesis, postherpetic neuralgia, depression or anxiety, which method comprises administration to a patient in need thereof of a therapeutically effective amount of a compound according to Claim 1.

12. Use of a compound as claimed in any one of Claims 1 to 7 for the manufacture of a medicament for the treatment or prevention of physiological disorders associated with an excess of tachykinins.

13. Use of a compound as claimed in any one of Claims 1 to 7 for the manufacture of a medicament for the treatment or prevention of pain or inflammation, migraine, emesis, postherpetic neuralgia, depression or anxiety.

14. A process for the preparation of a compound as claimed in any one of Claims 1 to 7.