WO2004078311A1 - A method of preparing ligands for hydrophobic interaction chromatography - Google Patents
A method of preparing ligands for hydrophobic interaction chromatography Download PDFInfo
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- WO2004078311A1 WO2004078311A1 PCT/SE2004/000316 SE2004000316W WO2004078311A1 WO 2004078311 A1 WO2004078311 A1 WO 2004078311A1 SE 2004000316 W SE2004000316 W SE 2004000316W WO 2004078311 A1 WO2004078311 A1 WO 2004078311A1
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- WIPO (PCT)
- Prior art keywords
- interaction
- ligand
- hic
- group
- ligands
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- 239000003446 ligand Substances 0.000 title claims abstract description 98
- 238000000034 method Methods 0.000 title claims abstract description 76
- 238000004191 hydrophobic interaction chromatography Methods 0.000 title claims abstract description 57
- 230000003993 interaction Effects 0.000 claims abstract description 70
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 46
- 239000011159 matrix material Substances 0.000 claims abstract description 37
- 238000000926 separation method Methods 0.000 claims abstract description 37
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 23
- 125000001165 hydrophobic group Chemical group 0.000 claims abstract description 9
- 125000002915 carbonyl group Chemical group [*:2]C([*:1])=O 0.000 claims abstract description 7
- 125000004122 cyclic group Chemical group 0.000 claims abstract description 7
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 6
- 238000005342 ion exchange Methods 0.000 claims abstract description 6
- 238000007098 aminolysis reaction Methods 0.000 claims abstract description 5
- 238000001212 derivatisation Methods 0.000 claims abstract description 5
- 238000004587 chromatography analysis Methods 0.000 claims description 18
- 230000002209 hydrophobic effect Effects 0.000 claims description 15
- 125000003396 thiol group Chemical class [H]S* 0.000 claims description 12
- 239000013076 target substance Substances 0.000 claims description 11
- KIWQWJKWBHZMDT-UHFFFAOYSA-N homocysteine thiolactone Chemical compound NC1CCSC1=O KIWQWJKWBHZMDT-UHFFFAOYSA-N 0.000 claims description 10
- 125000003903 2-propenyl group Chemical group [H]C([*])([H])C([H])=C([H])[H] 0.000 claims description 9
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- 230000008569 process Effects 0.000 claims description 8
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- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 claims description 4
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- 229910000160 potassium phosphate Inorganic materials 0.000 description 1
- 235000011009 potassium phosphates Nutrition 0.000 description 1
- IVRIRQXJSNCSPQ-UHFFFAOYSA-N propan-2-yl carbonochloridate Chemical compound CC(C)OC(Cl)=O IVRIRQXJSNCSPQ-UHFFFAOYSA-N 0.000 description 1
- 238000001742 protein purification Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- HDOUGSFASVGDCS-UHFFFAOYSA-N pyridin-3-ylmethanamine Chemical compound NCC1=CC=CN=C1 HDOUGSFASVGDCS-UHFFFAOYSA-N 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 238000007142 ring opening reaction Methods 0.000 description 1
- 125000006850 spacer group Chemical group 0.000 description 1
- 238000009987 spinning Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 150000003440 styrenes Chemical class 0.000 description 1
- 125000000446 sulfanediyl group Chemical group *S* 0.000 description 1
- FDDDEECHVMSUSB-UHFFFAOYSA-N sulfanilamide Chemical compound NC1=CC=C(S(N)(=O)=O)C=C1 FDDDEECHVMSUSB-UHFFFAOYSA-N 0.000 description 1
- 229940124530 sulfonamide Drugs 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 150000003568 thioethers Chemical class 0.000 description 1
- 125000005490 tosylate group Chemical group 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 229920001567 vinyl ester resin Polymers 0.000 description 1
- 125000000391 vinyl group Chemical group [H]C([*])=C([H])[H] 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/30—Processes for preparing, regenerating, or reactivating
- B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
- B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
- B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
- B01J20/3285—Coating or impregnation layers comprising different type of functional groups or interactions, e.g. different ligands in various parts of the sorbent, mixed mode, dual zone, bimodal, multimodal, ionic or hydrophobic, cationic or anionic, hydrophilic or hydrophobic
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/32—Bonded phase chromatography
- B01D15/325—Reversed phase
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/32—Bonded phase chromatography
- B01D15/325—Reversed phase
- B01D15/327—Reversed phase with hydrophobic interaction
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
- B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
Landscapes
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Organic Chemistry (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Pyridine Compounds (AREA)
- Heterocyclic Compounds Containing Sulfur Atoms (AREA)
- Furan Compounds (AREA)
- Peptides Or Proteins (AREA)
Abstract
Description
Claims
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP04717913A EP1599265A1 (en) | 2003-03-05 | 2004-03-05 | A method of preparing ligands for hydrophobic interaction chromatography |
US10/547,570 US7320755B2 (en) | 2003-03-05 | 2004-03-05 | Method of preparing ligands for hydrophobic interaction chromatography |
JP2006507945A JP4570052B2 (en) | 2003-03-05 | 2004-03-05 | Preparation of ligands for hydrophobic interaction chromatography |
CA2517757A CA2517757C (en) | 2003-03-05 | 2004-03-05 | A method of preparing ligands for hydrophobic interaction chromatography |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SE0300612-9 | 2003-03-05 | ||
SE0300612A SE0300612D0 (en) | 2003-03-05 | 2003-03-05 | A method of preparing ligands for hydrophobic interaction chromatography |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2004078311A1 true WO2004078311A1 (en) | 2004-09-16 |
Family
ID=20290597
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/SE2004/000316 WO2004078311A1 (en) | 2003-03-05 | 2004-03-05 | A method of preparing ligands for hydrophobic interaction chromatography |
Country Status (6)
Country | Link |
---|---|
US (1) | US7320755B2 (en) |
EP (1) | EP1599265A1 (en) |
JP (1) | JP4570052B2 (en) |
CA (1) | CA2517757C (en) |
SE (1) | SE0300612D0 (en) |
WO (1) | WO2004078311A1 (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2007137752A1 (en) * | 2006-05-26 | 2007-12-06 | Ge Healthcare Bio-Sciences Ab | A method for generating metal chelating affinity ligands |
EP2684951A1 (en) | 2012-07-13 | 2014-01-15 | Sandoz Ag | Method for producing a recombinant protein of interest |
CN107513117A (en) * | 2017-08-24 | 2017-12-26 | 中国科学技术大学 | A kind of multi-functional non-viral gene delivery vehicles polymer constructed based on thiolactone chemistry |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE602004018616D1 (en) * | 2003-03-05 | 2009-02-05 | Ge Healthcare Bio Sciences Ab | PROCESS FOR THE PRODUCTION OF MULTIMODAL ANION EXCHANGE LIGANDS |
SE0300612D0 (en) * | 2003-03-05 | 2003-03-05 | Amersham Biosciences Ab | A method of preparing ligands for hydrophobic interaction chromatography |
CA2687930C (en) * | 2007-05-25 | 2016-05-17 | Merck Patent Gesellschaft Mit Beschraenkter Haftung | Graft copolymers for cation exchange chromatography |
EP2470555B1 (en) * | 2009-10-30 | 2014-12-24 | Xellia Pharmaceuticals ApS | Method for purification of colistin and purified colistin components |
EP3055060A4 (en) * | 2013-10-10 | 2017-05-24 | GE Healthcare BioProcess R&D AB | Method for production of a chromatography material |
SG11201900201YA (en) | 2016-08-16 | 2019-02-27 | Regeneron Pharma | Methods for quantitating individual antibodies from a mixture |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4837348A (en) * | 1986-04-30 | 1989-06-06 | Varian Associates, Inc. | Bonded phase chromatographic supports |
WO2002053288A2 (en) * | 2000-12-31 | 2002-07-11 | Amersham Biosciences Ab | A method for the manufacture of compositions containing low concentrations of salts |
WO2003024588A1 (en) * | 2001-09-14 | 2003-03-27 | Amersham Biosciences Ab | Generation of ion exchanger media |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
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US4434151A (en) * | 1982-11-08 | 1984-02-28 | Medi-Physics, Inc. | Bifunctional chelating agents |
US5237016A (en) * | 1989-01-05 | 1993-08-17 | Siska Diagnostics, Inc. | End-attachment of oligonucleotides to polyacrylamide solid supports for capture and detection of nucleic acids |
US5652348A (en) * | 1994-09-23 | 1997-07-29 | Massey University | Chromatographic resins and methods for using same |
JP2000351776A (en) * | 1999-04-08 | 2000-12-19 | Kuraray Co Ltd | Production of optically active homocysteinethiolactone salt and its intermediate |
SE9904197D0 (en) * | 1999-11-22 | 1999-11-22 | Amersham Pharm Biotech Ab | An method for anion exchange adsorption on matrices carrying mixed mode ligands |
GB2384640B (en) * | 2002-01-29 | 2004-04-14 | Simpleware Ltd | Image processing method |
SE526214C2 (en) * | 2003-02-28 | 2005-07-26 | Amersham Biosciences Ab | One way to generate metal chelating affinity ligands |
SE0300624D0 (en) * | 2003-03-05 | 2003-03-05 | Amersham Biosciences Ab | A method of preparing affinity ligands |
SE0300612D0 (en) * | 2003-03-05 | 2003-03-05 | Amersham Biosciences Ab | A method of preparing ligands for hydrophobic interaction chromatography |
-
2003
- 2003-03-05 SE SE0300612A patent/SE0300612D0/en unknown
-
2004
- 2004-03-05 US US10/547,570 patent/US7320755B2/en not_active Expired - Fee Related
- 2004-03-05 WO PCT/SE2004/000316 patent/WO2004078311A1/en active Application Filing
- 2004-03-05 EP EP04717913A patent/EP1599265A1/en not_active Withdrawn
- 2004-03-05 JP JP2006507945A patent/JP4570052B2/en not_active Expired - Fee Related
- 2004-03-05 CA CA2517757A patent/CA2517757C/en not_active Expired - Fee Related
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4837348A (en) * | 1986-04-30 | 1989-06-06 | Varian Associates, Inc. | Bonded phase chromatographic supports |
WO2002053288A2 (en) * | 2000-12-31 | 2002-07-11 | Amersham Biosciences Ab | A method for the manufacture of compositions containing low concentrations of salts |
WO2003024588A1 (en) * | 2001-09-14 | 2003-03-27 | Amersham Biosciences Ab | Generation of ion exchanger media |
Non-Patent Citations (1)
Title |
---|
FEIST P.L. ET AL.: "Sulfhydrylcellulose: A New Medium for Chromatography of Mercurated Polynucleotides", BIOCHEMISTRY, vol. 20, no. 15, 21 July 1981 (1981-07-21), pages 4243 - 4246, XP002980033 * |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2007137752A1 (en) * | 2006-05-26 | 2007-12-06 | Ge Healthcare Bio-Sciences Ab | A method for generating metal chelating affinity ligands |
US7988858B2 (en) | 2006-05-26 | 2011-08-02 | Ge Healthcare Bio-Sciences Ab | Method for generating metal chelating affinity ligands |
EP2684951A1 (en) | 2012-07-13 | 2014-01-15 | Sandoz Ag | Method for producing a recombinant protein of interest |
WO2014009515A1 (en) | 2012-07-13 | 2014-01-16 | Sandoz Ag | Method for producing a recombinant protein of interest |
CN107513117A (en) * | 2017-08-24 | 2017-12-26 | 中国科学技术大学 | A kind of multi-functional non-viral gene delivery vehicles polymer constructed based on thiolactone chemistry |
CN107513117B (en) * | 2017-08-24 | 2019-08-27 | 中国科学技术大学 | A kind of multi-functional non-viral gene delivery vehicles polymer constructed based on thiolactone chemistry |
Also Published As
Publication number | Publication date |
---|---|
US7320755B2 (en) | 2008-01-22 |
SE0300612D0 (en) | 2003-03-05 |
CA2517757C (en) | 2011-07-19 |
US20060175258A1 (en) | 2006-08-10 |
EP1599265A1 (en) | 2005-11-30 |
CA2517757A1 (en) | 2004-09-16 |
JP4570052B2 (en) | 2010-10-27 |
JP2006523308A (en) | 2006-10-12 |
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