WO2004076059A2 - System and process for the synthesis of polymers - Google Patents
System and process for the synthesis of polymers Download PDFInfo
- Publication number
- WO2004076059A2 WO2004076059A2 PCT/EP2004/002040 EP2004002040W WO2004076059A2 WO 2004076059 A2 WO2004076059 A2 WO 2004076059A2 EP 2004002040 W EP2004002040 W EP 2004002040W WO 2004076059 A2 WO2004076059 A2 WO 2004076059A2
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- WIPO (PCT)
- Prior art keywords
- polymer
- building block
- substrate
- groups
- microdroplet
- Prior art date
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- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/12—Libraries containing saccharides or polysaccharides, or derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
- C40B50/14—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
- C40B60/14—Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B70/00—Tags or labels specially adapted for combinatorial chemistry or libraries, e.g. fluorescent tags or bar codes
Definitions
- US Patent 6,258,454 discloses low surface energy functionalized surfaces on solid glass supports (slides) by treating the glass slides having hydrophilic moieties on its surface with a derivatizing composition containing a mixture of silanes.
- a first silane provides the desired reduction in surface energy, while the second silane enables functionalization with molecular moieties of interest such as initial monomers to be used in the solid phase synthesis of oligomers.
- US Patent 6,184,347 discloses a wash reagent employed for the bulk washing of the surface of high-density polymer arrays to remove unreacted polymeric building blocks from cells of the array while at the same time reacting with the unreacted monomer in order to prevent reaction of the reacted monomer with functional groups on the surface of the HDA outside of the region of the surface to which the reactive monomer is applied. Therefore, blooming of the droplets applied to the surface of a high-density array is minimized.
- the reactive wash solution is preferably methanol.
- adjacent means that the micro mixing vessel is either in spatial vicinity to the ink-jet print head and linked e.g. via tubes to the ink-jet print head or, the micro mixing vessel is a part or an integral part of the ink-jet print head and may be separated from the head or from the nozzles via solid phase filters frits, diaphragms and the like.
- the chemically inert species obtained after reacting an appropriate agent with the unreacted surface functional groups contains phosphorous. It is especially preferred that the phosphorous containing agent forms a phosphate group upon reaction with the reactive functional groups on the surface.
- the thus obtained reaction product forms an inert, especially chemically inert, surface between the reaction sites (spots) which are defined by the location of the first microdroplet.
- perfluorated phosphorous derivatives are used which create inert and hydrophobic zones around the reaction sites. The hydrophobicity has the advantageous effect, that the second etc droplets will be centered on the non hydrophobic portion, i.e. the spots of the substrate. Also, a blooming out is efficiently avoided.
- the substrate is an organic polymer comprising activated groups like OH, NRH, SH and the like so as to provide a plurality of attachment sites for various polymer building blocks with different reactive groups without requiring the introduction of specific linker moieties.
- the activation is achieved via different mechanisms essentially known to a person skilled in the art, for example via plasma treatment, laser treatment and the like.
- suitable amino protecting groups include but are not limited to 2-(4-biphenyl)propyl[2]oxycarbonyl (Bpoc), 1-(1- adamantyl)-l-methylethoxy-carbonyl (Adpoc) and the like.
- Representative activators or so-called in-situ coupling reagent suitable for use in the present invention include but are not limited to N,N'-dicyclohexylcarbodiimide (DCC) and the like.
- This especially preferred embodiment not only uses micro mixing vessel 610 for the addition of the mixture between the phosphorous building block, for example a phosphor amidite, and an activator but also after reaction for washing the substrate with a wash solvent. Therefore, the set-up can be further simplified and made easier.
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Nanotechnology (AREA)
- Analytical Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Crystallography & Structural Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Materials Engineering (AREA)
- General Physics & Mathematics (AREA)
- Condensed Matter Physics & Semiconductors (AREA)
- Composite Materials (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Other Resins Obtained By Reactions Not Involving Carbon-To-Carbon Unsaturated Bonds (AREA)
Abstract
Description
Claims
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US10/547,129 US20060263534A1 (en) | 2003-02-28 | 2004-03-01 | System and process for the synthesis of polymers |
JP2006501979A JP2006519285A (en) | 2003-02-28 | 2004-03-01 | Systems and methods for polymer synthesis |
EP04715888A EP1606047A2 (en) | 2003-02-28 | 2004-03-01 | System and process for the synthesis of polymers |
US12/634,204 US20100145014A1 (en) | 2003-02-28 | 2009-12-09 | System and process for the synthesis of polymers |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE10308931.4 | 2003-02-28 | ||
DE2003108931 DE10308931A1 (en) | 2003-02-28 | 2003-02-28 | System and method for the synthesis of polymers |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US12/634,204 Division US20100145014A1 (en) | 2003-02-28 | 2009-12-09 | System and process for the synthesis of polymers |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2004076059A2 true WO2004076059A2 (en) | 2004-09-10 |
WO2004076059A3 WO2004076059A3 (en) | 2004-12-23 |
Family
ID=32891807
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/EP2004/002040 WO2004076059A2 (en) | 2003-02-28 | 2004-03-01 | System and process for the synthesis of polymers |
Country Status (5)
Country | Link |
---|---|
US (2) | US20060263534A1 (en) |
EP (1) | EP1606047A2 (en) |
JP (1) | JP2006519285A (en) |
DE (1) | DE10308931A1 (en) |
WO (1) | WO2004076059A2 (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1459802A2 (en) * | 2003-03-21 | 2004-09-22 | Agilent Technologies, Inc. | Methods for fabricating biopolymer arrays |
WO2006079093A2 (en) * | 2005-01-24 | 2006-07-27 | Hewlett-Packard Development Company, L.P. | A system and a method for synthesizing nanoparticle arrays in-situ |
WO2008035272A2 (en) * | 2006-09-21 | 2008-03-27 | Koninklijke Philips Electronics N.V. | Ink-jet device and method for producing a biological assay substrate using a printing head and means for accelerated motion |
Families Citing this family (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE102004024364A1 (en) * | 2004-05-17 | 2005-12-15 | Apibio Sas | Process for the preparation of polymers |
WO2011102914A2 (en) * | 2010-02-22 | 2011-08-25 | Oligoco | Automated polymer-synthesis system |
US8361396B2 (en) | 2010-02-22 | 2013-01-29 | Oligoco, Inc. | Automated polymer-synthesis system |
US10650312B2 (en) | 2016-11-16 | 2020-05-12 | Catalog Technologies, Inc. | Nucleic acid-based data storage |
KR102534408B1 (en) | 2016-11-16 | 2023-05-18 | 카탈로그 테크놀로지스, 인크. | Nucleic acid-based data storage |
KR20200132921A (en) | 2018-03-16 | 2020-11-25 | 카탈로그 테크놀로지스, 인크. | Chemical methods for storing nucleic acid-based data |
AU2019266303A1 (en) * | 2018-05-09 | 2021-01-07 | Vibrant Holdings, Llc | Methods of synthesizing a polynucleotide array using photactivated agents |
WO2019222561A1 (en) | 2018-05-16 | 2019-11-21 | Catalog Technologies, Inc. | Compositions and methods for nucleic acid-based data storage |
WO2020227718A1 (en) | 2019-05-09 | 2020-11-12 | Catalog Technologies, Inc. | Data structures and operations for searching, computing, and indexing in dna-based data storage |
WO2021072398A1 (en) | 2019-10-11 | 2021-04-15 | Catalog Technologies, Inc. | Nucleic acid security and authentication |
KR20230008877A (en) | 2020-05-11 | 2023-01-16 | 카탈로그 테크놀로지스, 인크. | Programs and functions of DNA-based data storage |
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WO1995025116A1 (en) * | 1994-03-16 | 1995-09-21 | California Institute Of Technology | Method and apparatus for performing multiple sequential reactions on a matrix |
US5508200A (en) * | 1992-10-19 | 1996-04-16 | Tiffany; Thomas | Method and apparatus for conducting multiple chemical assays |
WO1998041531A2 (en) * | 1997-03-20 | 1998-09-24 | University Of Washington | Solvent for biopolymer synthesis, solvent microdroplets and methods of use |
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US6461812B2 (en) * | 1998-09-09 | 2002-10-08 | Agilent Technologies, Inc. | Method and multiple reservoir apparatus for fabrication of biomolecular arrays |
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GB874665A (en) * | 1959-01-26 | 1961-08-10 | Metal Box Co Ltd | Improvements in or relating to apparatus for setting-up cartons |
US5847674A (en) * | 1996-05-02 | 1998-12-08 | Moore Business Forms, Inc. | Apparatus and methods for maintaining optimum print quality in an ink jet printer after periods of inactivity |
US6309828B1 (en) * | 1998-11-18 | 2001-10-30 | Agilent Technologies, Inc. | Method and apparatus for fabricating replicate arrays of nucleic acid molecules |
KR20010101196A (en) * | 1998-12-14 | 2001-11-14 | 하. 쭈어 하우젠 ; 요트. 후프타 | Method and devices for applying substances to a support, especially monomers for the combinatorial synthesis of molecule libraries |
US6171797B1 (en) * | 1999-10-20 | 2001-01-09 | Agilent Technologies Inc. | Methods of making polymeric arrays |
JP2003219872A (en) * | 2002-01-30 | 2003-08-05 | Takahiro Sato | Apparatus for freely arranging dna, rna or protein by arranging nucleotide by inkjet printing method |
-
2003
- 2003-02-28 DE DE2003108931 patent/DE10308931A1/en not_active Ceased
-
2004
- 2004-03-01 US US10/547,129 patent/US20060263534A1/en not_active Abandoned
- 2004-03-01 EP EP04715888A patent/EP1606047A2/en not_active Withdrawn
- 2004-03-01 JP JP2006501979A patent/JP2006519285A/en active Pending
- 2004-03-01 WO PCT/EP2004/002040 patent/WO2004076059A2/en active Application Filing
-
2009
- 2009-12-09 US US12/634,204 patent/US20100145014A1/en not_active Abandoned
Patent Citations (6)
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US5508200A (en) * | 1992-10-19 | 1996-04-16 | Tiffany; Thomas | Method and apparatus for conducting multiple chemical assays |
WO1995025116A1 (en) * | 1994-03-16 | 1995-09-21 | California Institute Of Technology | Method and apparatus for performing multiple sequential reactions on a matrix |
WO1998041531A2 (en) * | 1997-03-20 | 1998-09-24 | University Of Washington | Solvent for biopolymer synthesis, solvent microdroplets and methods of use |
US6461812B2 (en) * | 1998-09-09 | 2002-10-08 | Agilent Technologies, Inc. | Method and multiple reservoir apparatus for fabrication of biomolecular arrays |
WO2000051818A1 (en) * | 1999-03-01 | 2000-09-08 | Gemplus | Graphic printing machine for card-type storage medium, method for printing said storage media and storage media |
EP1270067A2 (en) * | 2001-06-29 | 2003-01-02 | Agilent Technologies, Inc. | Methods and apparatus for manufacturing arrays |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1459802A2 (en) * | 2003-03-21 | 2004-09-22 | Agilent Technologies, Inc. | Methods for fabricating biopolymer arrays |
EP1459802A3 (en) * | 2003-03-21 | 2005-04-13 | Agilent Technologies, Inc. | Methods for fabricating biopolymer arrays |
WO2006079093A2 (en) * | 2005-01-24 | 2006-07-27 | Hewlett-Packard Development Company, L.P. | A system and a method for synthesizing nanoparticle arrays in-situ |
WO2006079093A3 (en) * | 2005-01-24 | 2007-01-18 | Hewlett Packard Development Co | A system and a method for synthesizing nanoparticle arrays in-situ |
WO2008035272A2 (en) * | 2006-09-21 | 2008-03-27 | Koninklijke Philips Electronics N.V. | Ink-jet device and method for producing a biological assay substrate using a printing head and means for accelerated motion |
WO2008035272A3 (en) * | 2006-09-21 | 2008-06-26 | Koninkl Philips Electronics Nv | Ink-jet device and method for producing a biological assay substrate using a printing head and means for accelerated motion |
Also Published As
Publication number | Publication date |
---|---|
EP1606047A2 (en) | 2005-12-21 |
DE10308931A1 (en) | 2004-09-23 |
US20060263534A1 (en) | 2006-11-23 |
WO2004076059A3 (en) | 2004-12-23 |
US20100145014A1 (en) | 2010-06-10 |
JP2006519285A (en) | 2006-08-24 |
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