WO2004072297A2 - Terminal-phosphate-labeled nucleotides and methods of use - Google Patents
Terminal-phosphate-labeled nucleotides and methods of use Download PDFInfo
- Publication number
- WO2004072297A2 WO2004072297A2 PCT/US2004/002785 US2004002785W WO2004072297A2 WO 2004072297 A2 WO2004072297 A2 WO 2004072297A2 US 2004002785 W US2004002785 W US 2004002785W WO 2004072297 A2 WO2004072297 A2 WO 2004072297A2
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- WIPO (PCT)
- Prior art keywords
- phosphate
- label
- labeled
- polyphosphate
- nucleic acid
- Prior art date
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Classifications
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6823—Release of bound markers
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/06—Pyrimidine radicals
- C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/16—Purine radicals
- C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
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- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
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- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B11/00—Diaryl- or thriarylmethane dyes
- C09B11/04—Diaryl- or thriarylmethane dyes derived from triarylmethanes, i.e. central C-atom is substituted by amino, cyano, alkyl
- C09B11/10—Amino derivatives of triarylmethanes
- C09B11/24—Phthaleins containing amino groups ; Phthalanes; Fluoranes; Phthalides; Rhodamine dyes; Phthaleins having heterocyclic aryl rings; Lactone or lactame forms of triarylmethane dyes
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- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
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- C09B15/00—Acridine dyes
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- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
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- C09B57/00—Other synthetic dyes of known constitution
- C09B57/02—Coumarine dyes
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- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
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- C09B69/00—Dyes not provided for by a single group of this subclass
- C09B69/10—Polymeric dyes; Reaction products of dyes with monomers or with macromolecular compounds
- C09B69/109—Polymeric dyes; Reaction products of dyes with monomers or with macromolecular compounds containing other specific dyes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
Abstract
Description
Claims
Priority Applications (5)
Application Number | Priority Date | Filing Date | Title |
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EP04707050A EP1590475A4 (en) | 2003-02-05 | 2004-01-30 | Terminal-phosphate-labeled nucleotides and methods of use |
CN200480003557.5A CN1973048B (en) | 2003-02-05 | 2004-01-30 | Terminal-phosphate-labeled nucleotides and methods of use |
AU2004211588A AU2004211588A1 (en) | 2003-02-05 | 2004-01-30 | Terminal-phosphate-labeled nucleotides and methods of use |
CA2513672A CA2513672C (en) | 2003-02-05 | 2004-01-30 | Terminal-phosphate-labeled nucleotides and methods of use |
JP2006503220A JP2007524358A (en) | 2003-02-05 | 2004-01-30 | Terminal phosphate-labeled nucleotide and method of use |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
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US10/358,818 US7223541B2 (en) | 2001-08-29 | 2003-02-05 | Terminal-phosphate-labeled nucleotides and methods of use |
US10/358,818 | 2003-02-05 |
Publications (2)
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WO2004072297A2 true WO2004072297A2 (en) | 2004-08-26 |
WO2004072297A3 WO2004072297A3 (en) | 2007-01-18 |
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PCT/US2004/002785 WO2004072297A2 (en) | 2003-02-05 | 2004-01-30 | Terminal-phosphate-labeled nucleotides and methods of use |
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JP (1) | JP2007524358A (en) |
CN (1) | CN1973048B (en) |
AU (1) | AU2004211588A1 (en) |
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Also Published As
Publication number | Publication date |
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JP2007524358A (en) | 2007-08-30 |
CN1973048B (en) | 2015-06-03 |
CA2513672C (en) | 2014-01-14 |
EP1590475A4 (en) | 2007-10-03 |
EP1590475A2 (en) | 2005-11-02 |
WO2004072297A3 (en) | 2007-01-18 |
US20030162213A1 (en) | 2003-08-28 |
AU2004211588A1 (en) | 2004-08-26 |
CA2513672A1 (en) | 2004-08-26 |
US7223541B2 (en) | 2007-05-29 |
CN1973048A (en) | 2007-05-30 |
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