WO2004046370A3 - Digital karyotyping - Google Patents

Digital karyotyping Download PDF

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Publication number
WO2004046370A3
WO2004046370A3 PCT/US2003/036502 US0336502W WO2004046370A3 WO 2004046370 A3 WO2004046370 A3 WO 2004046370A3 US 0336502 W US0336502 W US 0336502W WO 2004046370 A3 WO2004046370 A3 WO 2004046370A3
Authority
WO
WIPO (PCT)
Prior art keywords
copy number
digital karyotyping
dna copy
provides
analysis
Prior art date
Application number
PCT/US2003/036502
Other languages
French (fr)
Other versions
WO2004046370A2 (en
Inventor
Tian-Li Wang
Victor Velculescu
Kenneth W Kinzler
Bert Vogelstein
Original Assignee
Univ Johns Hopkins
Tian-Li Wang
Victor Velculescu
Kenneth W Kinzler
Bert Vogelstein
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Johns Hopkins, Tian-Li Wang, Victor Velculescu, Kenneth W Kinzler, Bert Vogelstein filed Critical Univ Johns Hopkins
Priority to AU2003290933A priority Critical patent/AU2003290933A1/en
Publication of WO2004046370A2 publication Critical patent/WO2004046370A2/en
Publication of WO2004046370A3 publication Critical patent/WO2004046370A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation

Abstract

Alterations in the genetic content of a cell underlie many human diseases, including cancers. A method called Digital Karyotyping provides quantitative analysis of DNA copy number at high resolution. This approach involves the isolation and enumeration of short sequence tags from specific genomic loci. Analysis of human cancer cells using this method identified gross chromosomal changes as well as amplifications and deletions, including regions not previously known to be altered. Foreign DNA sequences not present in the normal human genome could also be readily identified. Digital Karyotyping provides a broadly applicable means for systematic detection of DNA copy number changes on a genomic scale.
PCT/US2003/036502 2002-11-15 2003-11-14 Digital karyotyping WO2004046370A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003290933A AU2003290933A1 (en) 2002-11-15 2003-11-14 Digital karyotyping

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US42640602P 2002-11-15 2002-11-15
US60/426,406 2002-11-15

Publications (2)

Publication Number Publication Date
WO2004046370A2 WO2004046370A2 (en) 2004-06-03
WO2004046370A3 true WO2004046370A3 (en) 2004-07-15

Family

ID=32326349

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/036502 WO2004046370A2 (en) 2002-11-15 2003-11-14 Digital karyotyping

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US (1) US7704687B2 (en)
AU (1) AU2003290933A1 (en)
WO (1) WO2004046370A2 (en)

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Publication number Publication date
AU2003290933A1 (en) 2004-06-15
US7704687B2 (en) 2010-04-27
US20040096892A1 (en) 2004-05-20
AU2003290933A8 (en) 2004-06-15
WO2004046370A2 (en) 2004-06-03

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