WO2004046100A3 - Release of the self-quenching of fluorescence near silver metallic surfaces - Google Patents

Release of the self-quenching of fluorescence near silver metallic surfaces Download PDF

Info

Publication number
WO2004046100A3
WO2004046100A3 PCT/US2003/035049 US0335049W WO2004046100A3 WO 2004046100 A3 WO2004046100 A3 WO 2004046100A3 US 0335049 W US0335049 W US 0335049W WO 2004046100 A3 WO2004046100 A3 WO 2004046100A3
Authority
WO
WIPO (PCT)
Prior art keywords
fluorophore
labeled
self
biomolecule
metallic particle
Prior art date
Application number
PCT/US2003/035049
Other languages
French (fr)
Other versions
WO2004046100A2 (en
Inventor
Joseph R Lakowicz
Joanna B Malicka
Ignacy Gryczynski
Original Assignee
Univ Maryland
Joseph R Lakowicz
Joanna B Malicka
Ignacy Gryczynski
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Maryland, Joseph R Lakowicz, Joanna B Malicka, Ignacy Gryczynski filed Critical Univ Maryland
Priority to AU2003291718A priority Critical patent/AU2003291718A1/en
Publication of WO2004046100A2 publication Critical patent/WO2004046100A2/en
Publication of WO2004046100A3 publication Critical patent/WO2004046100A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
    • G01N33/553—Metal or metal coated
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label

Abstract

Materials, systems and methods for reducing self-quenching due to homo resonance energy transfer when biomolecules are labeled with more than one fluorophore. The materials include a fluorophore labeled biomolecule conjugate and a metallic particle, wherein the labeled biomolecule conjugate comprises multiple fluorophores per biomolecule. The fluorophore-biomolecule conjugate may be bound to the metallic particle or positioned at a distance from the metallic particle, wherein the position of the fluorophore labeled biomolecule conjugate to the metallic particle reduces self-quenching due to homo resonance energy transfer. The methods include detecting a presence of or quantifying an amount of a biomolecule. The fluorophore labeled biomolecules and metallic particles can be used to obtain ultra-bright reagents for use in immunoassays, imaging, arrays and other applications.
PCT/US2003/035049 2002-11-15 2003-11-17 Release of the self-quenching of fluorescence near silver metallic surfaces WO2004046100A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003291718A AU2003291718A1 (en) 2002-11-15 2003-11-17 Release of the self-quenching of fluorescence near silver metallic surfaces

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US42677802P 2002-11-15 2002-11-15
US60/426,778 2002-11-15

Publications (2)

Publication Number Publication Date
WO2004046100A2 WO2004046100A2 (en) 2004-06-03
WO2004046100A3 true WO2004046100A3 (en) 2004-09-30

Family

ID=32326423

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/035049 WO2004046100A2 (en) 2002-11-15 2003-11-17 Release of the self-quenching of fluorescence near silver metallic surfaces

Country Status (2)

Country Link
AU (1) AU2003291718A1 (en)
WO (1) WO2004046100A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7871573B2 (en) 2004-07-26 2011-01-18 University Of Louisville Research Foundation, Inc. Enhancement of sensitivity of fluorophore mediated biosensing and bioimaging
US8563328B2 (en) 2004-07-26 2013-10-22 University Of Louisville Research Foundation, Inc. Fiber-optic biosensor and biosensing methods
GB2431233A (en) * 2005-10-14 2007-04-18 E2V Tech Molecular detector arrangement
US9140707B2 (en) 2007-08-10 2015-09-22 University Of Louisville Research Foundation, Inc. Sensors and methods for detecting diseases caused by a single point mutation

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5501985A (en) * 1990-07-18 1996-03-26 Abbott Laboratories Analyte-substitute reagent for use in specific binding assay methods, devices and kits
US6284465B1 (en) * 1999-04-15 2001-09-04 Agilent Technologies, Inc. Apparatus, systems and method for locating nucleic acids bound to surfaces
US6312906B1 (en) * 1999-01-15 2001-11-06 Imperial College Innovations, Ltd. Immobilized nucleic acid hybridization reagent and method
US6327410B1 (en) * 1997-03-14 2001-12-04 The Trustees Of Tufts College Target analyte sensors utilizing Microspheres
US6369206B1 (en) * 1994-07-29 2002-04-09 Robert D. Leone Metal organothiol particles
US6586193B2 (en) * 1996-04-25 2003-07-01 Genicon Sciences Corporation Analyte assay using particulate labels

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5501985A (en) * 1990-07-18 1996-03-26 Abbott Laboratories Analyte-substitute reagent for use in specific binding assay methods, devices and kits
US6369206B1 (en) * 1994-07-29 2002-04-09 Robert D. Leone Metal organothiol particles
US6586193B2 (en) * 1996-04-25 2003-07-01 Genicon Sciences Corporation Analyte assay using particulate labels
US6327410B1 (en) * 1997-03-14 2001-12-04 The Trustees Of Tufts College Target analyte sensors utilizing Microspheres
US6312906B1 (en) * 1999-01-15 2001-11-06 Imperial College Innovations, Ltd. Immobilized nucleic acid hybridization reagent and method
US6284465B1 (en) * 1999-04-15 2001-09-04 Agilent Technologies, Inc. Apparatus, systems and method for locating nucleic acids bound to surfaces

Also Published As

Publication number Publication date
WO2004046100A2 (en) 2004-06-03
AU2003291718A1 (en) 2004-06-15
AU2003291718A8 (en) 2004-06-15

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