WO2004041810A1 - Compounds useful as inhibitors of jak and other protein kinases - Google Patents

Compounds useful as inhibitors of jak and other protein kinases Download PDF

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WO2004041810A1
WO2004041810A1 PCT/US2003/035188 US0335188W WO2004041810A1 WO 2004041810 A1 WO2004041810 A1 WO 2004041810A1 US 0335188 W US0335188 W US 0335188W WO 2004041810 A1 WO2004041810 A1 WO 2004041810A1
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optionally substituted
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Randy S. Bethiel
Young Choon Moon
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Vertex Pharmaceuticals Incorporated
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Priority to JP2004550489A priority Critical patent/JP2006508107A/en
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Priority to EP03778111A priority patent/EP1560824A1/en
Priority to AU2003286895A priority patent/AU2003286895A1/en
Priority to US10/702,113 priority patent/US7348335B2/en
Publication of WO2004041810A1 publication Critical patent/WO2004041810A1/en
Priority to AU2010246324A priority patent/AU2010246324B2/en

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Definitions

  • the present invention relates to compounds useful as inhibitors of protein kinases.
  • the invention also provides pharmaceutically acceptable compositions comprising the compounds of the invention and methods of using the compositions in the treatment of various disorders.
  • Protein kinases constitute a large family of structurally related enzymes that are responsible for the control of a variety of signal transduction processes within the cell. (See, Hardie, G. and Hanks, S. The Protein Kinase Facts Book, I and II, Academic Press, San Diego, CA: 1995). Protein kinases are thought to have evolved from a common ancestral gene due to the conservation of their structure and catalytic function. Almost all kinases contain a similar 250-300 amino acid catalytic domain. The kinases may be categorized into families by the substrates they phosphorylate (e.g., protein-tyrosine, protein-serine/threonine, lipids, etc.).
  • phosphorylate e.g., protein-tyrosine, protein-serine/threonine, lipids, etc.
  • the Janus kinases are a family of tyrosine kinases consisting of JAK1, JAK2, JAK3 and TYK2.
  • the JAKs play a critical role in cytokine signaling.
  • the downstream substrates of the JAK family of kinases include the signal transducer and activator of transcription (STAT) proteins.
  • STAT signal transducer and activator of transcription
  • JAK/STAT signaling has been implicated in the mediation of many abnormal immune responses such as allergies, asthma, autoimmune diseases such as transplant rejection, rheumatoid arthritis, amyotrophic lateral sclerosis and multiple sclerosis as well as in solid and hematologic malignancies such as leukemias and lymphomas.
  • the pharmaceutical intervention in the JAK/STAT pathway has been reviewed [Frank Mol. Med. 5, 432-456 (1999) & Seidel, et al, Oncogene 19, 2645-2656 (2000)].
  • JAK1, JAK2, and TYK2 are ubiquitously expressed, while JAK3 is predominantly expressed in hematopoietic cells.
  • JAK3 binds exclusively to the common cytokine receptor gamma chain ( ⁇ c ) and is activated by IL-2, IL-4, IL-7, IL-9, and IL-15.
  • ⁇ c common cytokine receptor gamma chain
  • the proliferation and survival of murine mast cells induced by IL-4 and IL-9 have, in fact, been shown to be dependent on JAK3- and ⁇ c - signaling [Suzuki et al, Blood 96, 2172-2180 (2000)].
  • IL-4-mediated STAT-phosphorylation has been implicated as the mechanism involved in early and late stages of rheumatoid arthritis (RA). Up-regulation of proinflammatory cytokines in RA synovium and synovial fluid is a characteristic of the disease. It has been demostrated that IL-4— mediated activation of IL-4/STAT pathway is mediated through the Janus Kinases (JAK 1 & 3) and that IL-4-associated JAK kinases are expressed in the RA synovium [Muller-Ladner, et al, J. Immunol 164, 3894-3901 (2000)].
  • Familial amyotrophic lateral sclerosis is a fatal neurodegenerative disorder affecting about 10% of ALS patients.
  • the survival rates of FALS mice were increased upon treatment with a JAK3 specific inhibitor. This suggested that JAK3 plays a role in FALS [Trieu, et al, Biochem. Biophys. Res. Commun. 267, 22-25 (2000)].
  • STAT Signal transducer and activator of transcription
  • JAK3 specific compounds were shown to inhibit the clonogenic growth of JAK3-expressing cell lines DAUDI, RAMOS, LC1;19, NALM-6, MOLT-3 and HL-60.
  • TEL/JAK2 fusion proteins have induced myeloproliferative disorders and in hematopoietic cell lines, introduction of TEL/JAK2 resulted in activation of STAT1, STAT3, STAT5, and cytokine-independent growth [Schwaller, et al, EMBO J. 17, 5321-5333 (1998)].
  • STAT3, STAT5, JAK1 and JAK2 were demonstrated to be constitutively activated in mouse T cell lymphoma characterized initially by LCK over-expression, thus further implicating the JAK/STAT pathway in abnormal cell growth [Yu, et al, J. Immunol. 159, 5206-5210 (1997)].
  • IL-6 -mediated STAT3 activation was blocked by an inhibitor of JAK, leading to sensitization of myeloma cells to apoptosis [Catlett-Falcone, et al, Immunity 10 , 105-115 (1999)].
  • Glycogen synthase kinase-3 (GSK-3) is a serine/threonine protein kinase comprised of and ⁇ isoforms that are each encoded by distinct genes [Coghlan et al., Chemistry & Biology, 7, 793-803 (2000); Kim and Kimmel, Curr. Opinion Genetics Dev., 10, 508-514 (2000)].
  • GSK-3 has been implicated in various diseases including diabetes, Alzheimer's disease, CNS disorders such as manic depressive disorder and neurodegenerative diseases, and cardiomyocete hypertrophy [WO 99/65897; WO 00/38675; and Haq et al., J. Cell Biol. (2000) 151, 117].
  • GSK-3 may be caused by, or result in, the abnormal operation of certain cell signaling pathways in which GSK-3 plays a role.
  • GSK-3 has been found to phosphorylate and modulate the activity of a number of regulatory proteins. These include glycogen synthase which is the rate limiting enzyme necessary for glycogen synthesis, the microtubule associated protein Tau, the gene transcription factor ⁇ - catenin, the translation initiation factor elF2B, as well as ATP citrate lyase, axin, heat shock factor- 1, c-Jun, c-Myc, c-Myb, CREB, and CEPB ⁇ . These diverse targets implicate GSK-3 in many aspects of cellular metabolism, proliferation, differentiation and development.
  • GSK-3 a GSK-3 mediated pathway that is relevant for the treatment of type II diabetes
  • insulin-induced signaling leads to cellular glucose uptake and glycogen synthesis.
  • GSK-3 is a negative regulator of the insulin-induced signal.
  • the presence of insulin causes inhibition of GSK-3 mediated phosphorylation and deactivation of glycogen synthase.
  • the inhibition of GSK-3 leads to increased glycogen synthesis and glucose uptake [Klein et al., PNAS, 93, 8455-9 (1996); Cross et al., Biochem. J., 303, 21-26 (1994); Cohen, Biochem. Soc. Trans., 21, 555-567 (1993); Massillon et al, Biochem J.
  • GSK-3 activity has also been associated with Alzheimer's disease. This disease is characterized by the well-known ⁇ -amyloid peptide and the formation of intracellular neurofibrillary tangles. The neurofibrillary tangles contain hyperphosphorylated Tau protein where Tau is phosphorylated on abnormal sites. GSK-3 has been shown to phosphorylate these abnormal sites in cell and animal models. Furthermore, inhibition of GSK-3 has been shown to prevent hyperphosphorylation of Tau in cells [Lovestone et al., Current Biology 4, 1077-86 (1994); Brownlees et al., Neuroreport 8, 3251-55 (1997)]. Therefore, it is believed that GSK-3 activity may promote generation of the neurofibrillary tangles and the progression of Alzheimer's disease.
  • ⁇ -catenin Another substrate of GSK-3 is ⁇ -catenin which is degradated after phosphorylation by GSK-3.
  • Reduced levels of ⁇ -catenin have been reported in schizophrenic patients and have also been associated with other diseases related to increase in neuronal cell death [Zhong et al., Nature, 395, 698-702 (1998); Takashima et al., PNAS, 90, 7789-93 (1993); Pei et al., J. Neuropathol Exp, 56, 70-78 (1997)].
  • Syk is a tyrosine kinase that plays a critical role in Fc ⁇ RI mediated mast cell degranulation and eosiniphil activation. Accordingly, Syk kinase is implicated in various allergic disorders, in particular asthma.It has been shown that that Syk binds to the phosphorylated gamma chain of the Fc ⁇ RI receptor via N-terminal SH2 domains and is essential for downstream signaling [Taylor et al, Mol Cell Biol 1995; 15:4149. [0020] Inhibition of eosinophil apoptosis has been proposed as key mechanisms for the development of blood and tissue eosinophilia in asthma.
  • IL-5 and GM-CSF are upregulated in asthma and are proposed to cause blood and tissue eosinophilia by inhibition of eosinophil apoptosis. Inhibition of eosinophil apoptosis has been proposed as a key mechanism for the development of blood and tissue eosinophilia in asthma. It has been reported that Syk kinase is required for the prevention of eosinophil apoptosis by cytokines (using antisense) [Yousefi et al, J Exp Med 1996; 183: 1407].
  • Cyclin-dependent kinases are serine/threonine protein kinases consisting of a ⁇ -sheet rich amino-terminal lobe and a larger carboxy-terminal lobe which is largely ⁇ - helical.
  • the CDKs display the 11 subdomains shared by all protein kinases and range in molecular mass from 33 to 44 kD.
  • This family of kinases which includes CDK1, CKD2, CDK4, and CDK6, requires phosphorylation at the residue corresponding to CDK2 Thrl60 in order to be fully active [Meijer, L., Drug Resistance Updates, 3, 83-88 (2000)].
  • Each CDK complex is formed from a regulatory cyclin subunit (e.g., cyclin A, Bl, B2, Dl, D2, D3, and E) and a catalytic kinase subunit (e.g., CDK1, CDK2, CDK4, CDK5, and CDK6).
  • a regulatory cyclin subunit e.g., cyclin A, Bl, B2, Dl, D2, D3, and E
  • a catalytic kinase subunit e.g., CDK1, CDK2, CDK4, CDK5, and CDK6.
  • Each different kinase/cyclin pair functions to regulate the different and specific phases of the cell cycle known as the Gl, S, G2, and M phases [Nigg, E., Nature Reviews, 2, 21-32 (2001); Flatt, P., Pietenpol, J., Drug Metabolism Reviews, 32, 283-305 (2000)].
  • the CDKs have been implicated in cell proliferation disorders, particularly in cancer.
  • cyclin Dl is commonly associated with numerous human cancers including breast, colon, hepatocellular carcinomas and gliomas [Flatt, P., Pietenpol, J., Drug Metabolism Reviews, 32, 283-305 (2000)].
  • the CDK2/cyclin E complex plays, a key role in the progression from the early Gi to S phases of the cell cycle and the overexpression of cyclin E has been associated with various solid tumors.
  • CDKs especially CDK2
  • CDK2 also play a role in apoptosis and T-cell development.
  • CDK2 has been identified as a key regulator of thymocyte apoptosis [Williams, O., et al, European Journal of Immunology, 709-713 (2000)].
  • Stimulation of CDK2 kinase activity is associated with the progression of apoptosis in thymocytes, in response to specific stimuli. Inhibition of CDK2 kinase activity blocks this apoptosis resulting in the protection of thymocytes.
  • CDKs are directly involved in the process of transcription. Numerous viruses require CDKs for their replication process. Examples where CDK inhibitors restrain viral replication include human cytomegakovirus, herpes virus, and varicella-zoster virus [Meijer, L., Drug Resistance Updates, 3, 83-88 (2000)].
  • Inhibition of CDK is also useful for the treatment of neurodegenerative disorders such as Alzheimer's disease.
  • the appearance of Paired Helical Filaments (PHF), associated with Alzheimer's disease, is caused by the hyperphosphorylation of Tau protein by CDK5/p25 [Meijer, L., Drug Resistance Updates, 3, 83-88 (2000)].
  • JNK is a member of the mitogen-activated protein (MAP) kinase family.
  • MAP kinases are activated by a variety of signals including growth factors, cytokines, UN radiation, and stress-inducing agents.
  • MAPKs are serine/threonine kinases and their activation occur by dual phosphorylation of threonine and tyrosine at the Thr-X-Tyr segment in the activation loop. MAPKs phosphorylate various substrates including transcription factors, which in turn regulate the expression of specific sets of genes and thus mediate a specific response to the stimulus.
  • J ⁇ K1, J ⁇ K2, JNK3 Three distinct genes, J ⁇ K1, J ⁇ K2, JNK3 have been identified for this kinase family and at least ten different splicing isoforms of JNKs exist in mammalian cells [Gupta et al, EMBO J., 15, 2760-70 (1996)].
  • Members of the JNK family are activated by proinflammatory cytokines, such as tumor necrosis factor- ⁇ (TNF ⁇ ) and interleukin-1 ⁇ (IL- l ⁇ ), as well as by environmental stress, including anisomycin, UN irradiation, hypoxia, and osmotic shock [Minden et al., Biochemica et Biophysica Acta, 1333, F85-F104 (1997)].
  • the down-stream substrates of J ⁇ Ks include transcription factors c-Jun, ATF-2, Elkl, p53 and a cell death domain protein (DE ⁇ ) [Zhang et al. Proc. Natl. Acad. Sci. USA, 95, 2586-91 (1998)].
  • Each J ⁇ K isoform binds to these substrates with different affinities, suggesting a regulation of signaling pathways by substrate specificity of different J ⁇ Ks in vivo (Gupta et al., supra).
  • J ⁇ Ks have been implicated in having a role in mediating cellular response to cancer, thrombin-induced platelet aggregation, immunodeficiency disorders, autoimmune diseases, cell death, allergies, osteoporosis and heart disease.
  • the therapeutic targets related to activation of the J ⁇ K pathway include chronic myelogenous leukemia (CML), rheumatoid arthritis, asthma, osteoarthritis, ischemia, cancer and neurodegenerative diseases.
  • CML chronic myelogenous leukemia
  • rheumatoid arthritis asthma, osteoarthritis, ischemia, cancer and neurodegenerative diseases.
  • JNK cascade also plays a role in T-cell activation, including activation of the IL-2 promoter.
  • inhibitors of JNK may have therapeutic value in altering pathologic immune responses [J. Immunol 162, 3176-87 (1999); Eur. J. Immunol. 28, 3867-77 (1998); J. Exp. Med. 186, 941-53 (1997); Eur. J. Immunol 26, 989-94 (1996)].
  • JNK activation in various cancers has also been established, suggesting the potential use of JNK inhibitors in cancer.
  • constitutively activated JNK is associated with HTLN-1 mediated tumorigenesis [Oncogene 13, 135-42 (1996)].
  • J ⁇ K may play a role in Kaposi's sarcoma (KS) because it is thought that the proliferative effects of ⁇ FGF and OSM on KS cells are mediated by their activation of the J ⁇ K signaling pathway [J. Clin. Invest. 99, 1798-804 (1997)].
  • J ⁇ K1 and J ⁇ K2 are widely expressed in a variety of tissues.
  • JNK3 is selectively expressed in the brain and to a lesser extent in the heart and testis [Gupta et al., supra; Mohit et al., Neuron 14, 67-78 (1995); Martin et al., Brain Res. Mol. Brain Res. 35, 47-57 (1996)].
  • JNK3 has been linked to neuronal apoptosis induced by kainic acid, indicating a role of JNK in the pathogenesis of glutamate neurotoxicity.
  • JNK3 expression is localized to a subpopulation of pyramidal neurons in the CA1, CA4 and subiculum regions of the hippocampus and layers 3 and 5 of the neocortex [Mohit et al., supra].
  • JNK3 appears to be involved involved in hypoxic and ischemic damage of CA1 neurons in the hippocampus.
  • JNK3 co-localizes immunochemically with neurons vulnerable in Alzheimer's disease [Mohit et al., supra].
  • JNK3 signaling pathway Disruption of the JNK3 gene caused resistance of mice to the excitotoxic glutamate receptor agonist kainic acid, including the effects on seizure activity, AP-1 transcriptional activity and apoptosis of hippocampal neurons, indicating that the JNK3 signaling pathway is a critical component in the pathogenesis of glutamate neurotoxicity (Yang et al., Nature, 389, ,865-870 (1997)].
  • JNK signalling especially that of JNK3, has been implicated in the areas of apoptosis-driven neurodegenerative diseases such as Alzheimer's Disease, Parkinson's Disease, ALS (Amyotrophic Lateral Sclerosis), epilepsy and seizures, Huntington's Disease, traumatic brain injuries, as well as ischemic and hemorrhaging stroke.
  • ALS Amyotrophic Lateral Sclerosis
  • epilepsy apoptosis-driven neurodegenerative diseases
  • Huntington's Disease Huntington's Disease
  • traumatic brain injuries as well as ischemic and hemorrhaging stroke.
  • CDK protein kinases are effective as inhibitors of
  • JAK-3, JNK-3, GSK-3, SYK, and CDK-2 protein kinases These compounds have the general formula I:
  • R 1 , T, R x , Z 1 , Z 2 , Z 3 , Z 4 , Z 5 , Z 6 , and Z 7 are as defined below.
  • compositions thereof are useful for treating or preventing a variety of disorders, such as heart disease, diabetes, Alzheimer's disease, immunodeficiency disorders, inflammatory diseases, allergic diseases, autoimmune diseases, destructive bone disorders such as osteoporosis, proliferative disorders, infectious diseases, immunologically-mediated diseases, and viral diseases.
  • the compositions are also useful in methods for preventing cell death and hyperplasia and therefore may be used to treat or prevent reperfusion/ischemia in stroke, heart attacks, and organ hypoxia.
  • the compositions are also useful in methods for preventing thrombin-induced platelet aggregation.
  • compositions are especially useful for disorders such as chronic myelogenous leukemia (CML), rheumatoid arthritis, asthma, osteoarthritis, ischemia, cancer, liver disease including hepatic ischemia, heart disease such as myocardial infarction and congestive heart failure, pathologic immune conditions involving T cell activation, and neurodegenerative disorders.
  • CML chronic myelogenous leukemia
  • rheumatoid arthritis rheumatoid arthritis
  • asthma chronic myelogenous leukemia
  • osteoarthritis ischemia
  • ischemia cancer
  • liver disease including hepatic ischemia
  • heart disease such as myocardial infarction and congestive heart failure
  • pathologic immune conditions involving T cell activation pathologic immune conditions involving T cell activation
  • neurodegenerative disorders such as chronic myelogenous leukemia (CML), rheumatoid arthritis, asthma, osteoarthritis, ischemia, cancer, liver disease including hepati
  • R 1 is Q-Ar 1 , wherein Q is a bond or is a C ⁇ . alkylidene chain wherein one methylene unit of Q is optionally replaced by O, NR, NRCO, NRCONR, NRCO 2 , CO, CO 2 , CONR, OCONR, SO 2 , SO 2 NR, NRSO 2 , NRSO 2 NR, COCO, or COCH 2 CO;
  • Ar 1 is a 5-7 membered saturated, partially unsaturated, or fully unsaturated monocyclic ring having 0-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or an 8-12 membered saturated, partially unsaturated, or fully unsaturated bicyclic ring system having 0-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; wherein Ar 1 is optionally substituted with q independent occurrences of Z-R ; wherein q is 0-5, Z is a bond or is a -C ⁇ alkylidene chain wherein up to two methylene units of Z are optionally and independently replaced by CO, CO 2 , COCO, CONR, OCONR, NRNR, NRNRCO, NRCO, NRCO 2 , NRCONR, SO, SO 2 , NRSO 2 , SO 2 NR, NRSO 2 NR, O, S, or NR; and each occurrence of R is independently selected from R', halogen, NO 2 , CN, OR',
  • Z 1 is N or CH
  • Z 7 is N or C-UR Y ;
  • T and U are each independently a bond or a saturated or unsaturated C ⁇ _ 6 alkylidene chain, wherein up to two methylene units of the chain are optionally and independently replaced by CO, CO 2 , COCO, CONR, OCONR, NRNR, NRNRCO, NRCO, NRCO 2 , NRCONR, SO, SO 2 , NRSO 2 , SO 2 NR, NRSO 2 NR, O, S, or NR;
  • R x and R ⁇ are each independently halogen, CN, NO 2 , or R';
  • Z 2 , Z 5 and Z 6 are each independently N or CH, provided that no more than two of Z 2 , Z 5 , and Z 6 are N;
  • Z 3 is CR 3 ;
  • Z 4 is CR 4 ; wherein one of R 3 or R 4 is R u , and the other of R 3 or R 4 is R V1 , wherein: R u is (CH 2 ) t CN, (CH 2 ) t NO 2 , (CH 2 ) t N(R) 2 , (CH 2 ) t NRC(O)R, (CH 2 ) t CON(R) 2 , (CH 2 ) t COOR, (CH 2 ) t SO 2 N(R) 2 , (CH 2 ) t NRSO 2 R, (CH 2 ) t NRCON(R) 2 , (CH 2 ) t NRSO 2 N(R) 2 , (CH 2 ) t COCOR, (CH 2 ) t Ar 2 , wherein t is 0, 1, or 2, and Ar 2 is an optionally substituted 5-7 membered saturated, partially unsaturated, or fully unsaturated monocyclic ring having 0-3 heteroatoms independently selected from nitrogen
  • compounds of the invention may optionally be substituted with one or more substituents, such as are illustrated generally above, or as exemplified by particular classes, subclasses, and species of the invention. It will be appreciated that the phrase “optionally substituted” is used interchangeably with the phrase “substituted or unsubstituted.” In general, the term “substituted”, whether preceded by the term “optionally” or not, refers to the replacement of hydrogen radicals in a given structure with the radical of a specified substituent.
  • an optionally substituted group may have a substituent at each substitutable position of the group, and when more than one position in any given structure may be substituted with more than one substituent selected from a specified group, the substituent may be either the same or different at every position.
  • Combinations of substituents envisioned by this invention are preferably those that result in the formation of stable or chemically feasible compounds.
  • stable refers to compounds that are not substantially altered when subjected to conditions to allow for their production, detection, and preferably their recovery, purification, and use for one or more of the purposes disclosed herein.
  • a stable compound or chemically feasible compound is one that is not substantially altered when kept at a temperature of 40°C or less, in the absence of moisture or other chemically reactive conditions, for at least a week.
  • aliphatic or "aliphatic group”, as used herein, means a straight-chain (i.e., unbranched) or branched, substituted or unsubstituted hydrocarbon chain that is completely saturated or that contains one or more units of unsaturation, or a monocyclic hydrocarbon or bicyclic hydrocarbon that is completely saturated or that contains one or more units of unsaturation, but which is not aromatic (also referred to herein as “carbocycle” "cycloaliphatic” or “cycloalkyl”), that has a single point of attachment to the rest of the molecule.
  • aliphatic groups contain 1-20 aliphatic carbon atoms.
  • aliphatic groups contain 1-10 aliphatic carbon atoms. In other embodiments, aliphatic groups contain 1-8 aliphatic carbon atoms. In still other embodiments, aliphatic groups contain 1-6 aliphatic carbon atoms, and in yet other embodiments aliphatic groups contain 1-4 aliphatic carbon atoms.
  • cycloaliphatic refers to a monocyclic C 3 -C 8 hydrocarbon or bicyclic C 8 -C 12 hydrocarbon that is completely saturated or that contains one or more units of unsaturation, but which is not aromatic, that has a single point of attachment to the rest of the molecule wherein any individual ring in said bicyclic ring system has 3-7 members.
  • Suitable aliphatic groups include, but are not limited to, linear or branched, substituted or unsubstituted alkyl, alkenyl, alkynyl groups and hybrids thereof such as (cycloalkyl)alkyl, (cycloalkenyl)alkyl or (cycloalkyl)alkenyl.
  • heteroaliphatic means aliphatic groups wherein one or two carbon atoms are independently replaced by one or more of oxygen, sulfur, nitrogen, phosphorus, or silicon. Heteroaliphatic groups may be substituted or unsubstituted, branched or unbranched, cyclic or acyclic, and include "heterocycle”, “heterocyclyl”, “heterocycloaliphatic”, or “heterocyclic” groups.
  • heterocycle means non-aromatic, monocyclic, bicyclic, or tricyclic ring systems in which one or more ring members are an independently selected heteroatom.
  • the "heterocycle”, “heterocyclyl”, “heterocycloaliphatic”, or “heterocyclic” group has three to fourteen ring members in which one or more ring members is a heteroatom independently selected from oxygen, sulfur, nitrogen, or phosphorus, and each ring in the system contains 3 to 7 ring members.
  • heteroatom means one or more of oxygen, sulfur, nitrogen, phosphorus, or silicon (including, any oxidized form of nitrogen, sulfur, phosphorus, or silicon; the quaternized form of any basic nitrogen or; a substitutable nitrogen of a heterocyclic ring, for example N (as in 3,4-dihydro-2H-pyrrolyl), N ⁇ (as in pyrrolidinyl) or
  • alkoxy refers to an alkyl group, as previously defined, attached to the principal carbon chain through an oxygen (“alkoxy”) or sulfur (“thioalkyl”) atom.
  • haloalkyl means alkyl, alkenyl or alkoxy, as the case may be, substituted with one or more halogen atoms.
  • halogen means F, Cl, Br, or I.
  • aryloxy refers to monocyclic, bicyclic, and tricyclic ring systems having a total of five to fourteen ring members, wherein at least one ring in the system is aromatic and wherein each ring in the system contains 3 to 7 ring members.
  • aryl may be used interchangeably with the term “aryl ring”.
  • aryl also refers to heteroaryl ring systems as defined hereinbelow.
  • heteroaryl used alone or as part of a larger moiety as in
  • heteroarylkyl refers to monocyclic, bicyclic, and tricyclic ring systems having a total of five to fourteen ring members, wherein at least one ring in the system is aromatic, at least one ring in the system contains one or more heteroatoms, and wherein each ring in the system contains 3 to 7 ring members.
  • heteroaryl may be used interchangeably with the term “heteroaryl ring” or the term “heteroaromatic".
  • suitable substituents on the unsaturated carbon atom of an aryl or heteroaryl group are generally selected from halogen; -R°; -OR°; -SR°; phenyl (Ph) optionally substituted with R°; -O(Ph) optionally substituted with R°; -(CH 2 ) ⁇ .
  • each independent occurrence of R° is selected from hydrogen, optionally substituted C ⁇ _ 6 aliphatic, an unsubstituted 5-6 membered heteroaryl or heterocyclic ring, phenyl, -O(Ph), or -CH (Ph), or, notwithstanding the definition above, two independent occurrences of R°, on the same substituent or different substituents, taken together with the atom(s) to which each R° group is bound, to form an optionally substituted 3-12 membered saturated, partially unsaturated, or fully unsaturated monocyclic or bicyclic ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur.
  • Optional substituents on the aliphatic group of R° are selected from NH 2 , NH(C ⁇ . 4 aliphatic), N(C 1 - aliphatic) 2 , halogen, NO 2 , CN, CO 2 H, CO 2 (C 1 _ 4 aliphatic), O aloC aliphatic), or wherein each of the foregoing C ⁇ aliphatic groups of R° is unsubstituted.
  • An aliphatic or heteroaliphatic group, or a non-aromatic heterocyclic ring may contain one or more substituents and thus may be "optionally substituted".
  • Optional substituents on the aliphatic group or the phenyl ring of R + are selected from -NH 2 , -NH(Ci_ 4 aliphatic), -N(C ⁇ _ 4 aliphatic) , halogen, _ aliphatic, -OH, -0(C ⁇ aliphatic), -NO 2 , -CN, -CO 2 H, -CO 2 (C M aliphatic), -O(halo C M aliphatic), or halo(C ⁇ _ 4 aliphatic), wherein each of the foregoing C ⁇ aliphatic groups of R is unsubstituted.
  • alkylidene chain refers to a straight or branched carbon chain that may be fully saturated or have one or more units of unsaturation and has two points of attachment to the rest of the molecule.
  • two independent occurrences of R° are taken together with the atom(s) to which they are bound to form an optionally substituted 3-12 membered saturated, partially unsaturated, or fully unsaturated monocyclic or bicyclic ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur.
  • Exemplary rings that are formed when two independent occurrences of R° (or R + , R, R' or any other variable similarly defined herein), are taken together with the atom(s) to which each variable is bound include, but are not limited to the following: a) two independent occurrences of R° (or R + , R, R' or any other variable similarly defined herein) that are bound to the same atom and are taken together with that atom to form a ring, for example, N(R°) 2 , where both occurrences of R° are taken together with the nitrogen atom to form a piperidin-1-yl, piperazin-1-yl, or morpholin-4-yl group; and b) two independent occurrences of R° (or R + , R, R' or any other variable similarly defined herein) that are bound to different atoms and are taken together with both of those atoms to form a ring, for example
  • a fused 6-membered oxygen containing ring It will be appreciated that a variety of other rings can be formed when two independent occurrences of R° (or R , R, R' or any other variable similarly defined herein) are taken together with the atom(s) to which each variable is bound and that the examples detailed above are not intended to be limiting. [0069] Unless otherwise stated, structures depicted herein are also meant to include all isomeric (e.g., enantiomeric, diastereomeric, and geometric (or conformational)) forms of the structure; for example, the R and S configurations for each asymmetric center, (Z) and (E) double bond isomers, and (Z) and (E) conformational isomers.
  • isomeric e.g., enantiomeric, diastereomeric, and geometric (or conformational
  • Z 1 is N and amino pyrimidines of general formula II are provided:
  • R , UR , TR , Z , Z , Z , Z , and Z are as defined generally above and in classes and subclasses herein.
  • Z 1 is CH and amino pyridines of general formula III are provided:
  • R 1 , UR Y , TR X , Z 2 , Z 3 , Z 4 , Z 5 , and Z 6 are as defined generally above and in classes and subclasses herein.
  • R 1 is Q-Ar 1 .
  • Certain exemplary substituents for R 1 include optionally substituted group selected from phenyl, cyclohexyl, cyclopentyl, pyridyl, naphthyl, morpholino, piperazinyl, or piperidinyl.
  • R 1 is an optionally substituted group selected from phenyl, cyclohexyl, or pyridyl.
  • R 1 is optionally substituted phenyl.
  • Ar 1 is substituted with 0-5 occurrences of ZR Z .
  • Exemplary ZR Z groups are selected from halogen, R', CN, NO 2 , - N(R)(R'), -OR', -SR', -S(O) 2 N(R)(R'), CO(C M alkyl), -NRSO 2 R', COO(C M alkyl).
  • q is 1 or 2 and ZR Z is F, Cl, Br, COO(C M alkyl), C ⁇ _ 4 alkyl, CN, NO 2 , -NH 2 , - OH, -S(O) 2 NH 2 , or an optionally substituted benzyloxy, phenyloxy, or phenyl group.
  • q is 1, and ZR Z is in the meta or para position and ZR Z is F, Cl, Br, methyl, ethyl, benzyloxy, phenyl, phenyloxy, COO(C 1 . alkyl), -NH 2 , -OH, ⁇ alkoxy, or -S(O) 2 NH 2 .
  • TR X and UR Y groups of formula I, and classes and subclasses thereof as described herein, are each independently hydrogen, halogen, NO 2 , CN, OR, SR or N(R) 2 , or C ⁇ _ aliphatic optionally substituted with oxo, OR, SR, N(R) , halogen, NO 2 or CN.
  • TR X and UR Y are each independently hydrogen, Me, OH, OMe or N(R) 2 .
  • TR and UR are each hydrogen.
  • ring B is selected from one of the rings i-viii depicted below.
  • ring B is i, ii, v, vi, or vii. In yet other embodiments,
  • R 3 or R 4 is R u
  • R V1 the other of R 3 or R is R V1
  • R u is (CH 2 ) t CN, (CH 2 ) t NO 2 , (CH 2 ) t N(R) 2 , (CH 2 ) t NRC(O)R, (CH 2 ) t CON(R) 2 , (CH 2 ) t COOR, (CH 2 ) t SO 2 N(R) 2 , (CH 2 ) t NRSO 2 R, (CH 2 ) t NRCON(R) 2 , (CH 2 ) t NRSO 2 N(R) 2 , (CH 2 ) t COCOR, (CH ⁇ t Ar 2 , wherein t is 0, 1, or 2, and Ar 2 is an optionally substituted 5-7 membered saturated, partially unsaturated, or fully unsaturated monocyclic ring having 0-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • Ar 2 is an optionally substituted tetrazole, triazole, oxazole, thiazole, thiadiazole, oxadiazole or pyridyl group.
  • compounds have one of the structures depicted below:
  • ring B is selected from one of structures i, ii, v, vi, or vii. In certain exemplary embodiments, ring B is selected from i.
  • R 1 is selected from an optionally substituted group selected from phenyl, cyclohexyl, cyclopentyl, pyridyl, morpholino, piperazinyl, or piperidinyl;
  • R 1 is an optionally substituted group selected from phenyl, cyclohexyl, or pyridyl;
  • ZR Z groups are selected from halogen, R', CN, NO 2 , -N(R)(R'), -OR', -SR', -S(O) 2 N(R)(R'),
  • TR X and UR Y are selected from hydrogen, halogen, NO 2 ,
  • xii) compounds where t is 1; and [0093] xiii) compounds where Ar 2 is an optionally substituted tetrazole, triazole, oxazole, thiazole, thiadiazole, oxadiazole or pyridyl group
  • Certain exemplary embodiments also relate to those compounds where ring A is a ppyyrriimmiiddiinnee;; UURR Y ,, aanndd TTRR S are each hydrogen, and the compounds have the general structures depicted generally below:
  • Certain exemplary subsets for each of the compounds described above include those compounds where: a. q is 0, 1, or 2, and ZR Z is halogen, R', CN, NO 2 , -N(R)(R'), -OR', -SR', - S(O) 2 N(R)(R'), CO(C M alkyl), -NRSO 2 R', COO(C w alkyl); b. t is O; c. R is optionally substituted C ⁇ _ 6 alkyl or hydrogen; and d. R' is optionally substituted C ⁇ _ 6 alkyl or hydrogen.
  • Schemes 1, 2 and 3 below depict generally the synthesis of certain exemplary compounds of the invention. Specifically, Scheme 1 depicts the synthesis of compounds where R 3 is COOH or CON(R) 2 and R 4 is OH. Scheme 2 depicts compounds where R 3 is
  • the present invention provides compounds that are inhibitors of protein kinases, and thus the present compounds are useful for the treatment of diseases, disorders, and conditions including, but not limited to a proliferative disorder, a cardiac disorder, a neurodegenerative disorder, psychotic disorders, an autoimmune disorder, a condition associated with organ transplant, an inflammatory disorder, an immunologically mediated disorder, a viral disease, or a bone disorder.
  • diseases, disorders, and conditions including, but not limited to a proliferative disorder, a cardiac disorder, a neurodegenerative disorder, psychotic disorders, an autoimmune disorder, a condition associated with organ transplant, an inflammatory disorder, an immunologically mediated disorder, a viral disease, or a bone disorder.
  • the compounds are useful for the treatment of allergy, asthma, diabetes, Alzheimer's disease, Huntington's disease, Parkinson's disease, AIDS-associated dementia, amyotrophic lateral sclerosis (AML, Lou Gehrig's disease), multiple sclerosis (MS), schizophrenia, cardiomyocyte hypertrophy, reperfusion/ischemia (e.g., stroke), baldness, cancer, hepatomegaly, cardiovascular disease including cardiomegaly, cystic fibrosis, viral disease, autoimmune diseases, atherosclerosis, restenosis, psoriasis, inflammation, hypertension, angina pectoris, cerebrovascular contraction, peripheral circulation disorder, premature birth, arteriosclerosis, vasospasm (cerebral vasospasm, coronary vasospasm), retinopathy, erectile dysfunction (ED), AIDS, osteoporosis, Crohn's Disease and colitis, neurite outgrowth, and Raynaud's Disease.
  • AML amyotrophic lateral sclerosis
  • the disease, condition, or disorder is atherosclerosis, hypertension, erectile dysfunction (ED), reperfusion/ischemia (e.g., stroke), or vasospasm (cerebral vasospasm and coronary vasospasm).
  • compositions comprising any of the compounds as described herein, and optionally comprise a pharmaceutically acceptable carrier, adjuvant or vehicle.
  • these compositions optionally further comprise one or more additional therapeutic agents.
  • a pharmaceutically acceptable derivative includes, but is not limited to, pharmaceutically acceptable prodrugs, salts, esters, salts of such esters, or any other adduct or derivative which upon administration to a patient in need is capable of providing, directly or indirectly, a compound as otherwise described herein, or a metabolite or residue thereof.
  • the term "pharmaceutically acceptable salt” refers to those salts which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of humans and lower animals without undue toxicity, irritation, allergic response and the like, and are commensurate with a reasonable benefit/risk ratio.
  • a “pharmaceutically acceptable salt” means any non-toxic salt or salt of an ester of a compound of this invention that, upon administration to a recipient, is capable of providing, either directly or indirectly, a compound of this invention or an inhibitorily active metabolite or residue thereof.
  • inhibitors as used herein, the term "inhibitorily active metabolite or residue thereof means that a metabolite or residue thereof is also an inhibitor of a JAK-3, JNK-3, CDK-2, SYK, or GSK-3 kinase.
  • Pharmaceutically acceptable salts are well known in the art. For example, S. M. Berge et al, describe pharmaceutically acceptable salts in detail in J. Pharmaceutical Sciences, 1977, 66, 1-19, incorporated herein by reference. Pharmaceutically acceptable salts of the compounds of this invention include those derived from suitable inorganic and organic acids and bases.
  • Examples of pharmaceutically acceptable, nontoxic acid addition salts are salts of an amino group formed with inorganic acids such as hydrochloric acid, hydrobromic acid, phosphoric acid, sulfuric acid and perchloric acid or with organic acids such as acetic acid, oxalic acid, maleic acid, tartaric acid, citric acid, succinic acid or malonic acid or by using other methods used in the art such as ion exchange.
  • inorganic acids such as hydrochloric acid, hydrobromic acid, phosphoric acid, sulfuric acid and perchloric acid
  • organic acids such as acetic acid, oxalic acid, maleic acid, tartaric acid, citric acid, succinic acid or malonic acid or by using other methods used in the art such as ion exchange.
  • salts include adipate, alginate, ascorbate, aspartate, benzenesulfonate, benzoate, bisulfate, borate, butyrate, camphorate, camphorsulfonate, citrate, cyclopentanepropionate, digluconate, dodecylsulfate, ethanesulfonate, formate, fumarate, glucoheptonate, glycerophosphate, gluconate, hemisulfate, heptanoate, hexanoate, hydroiodide, 2-hydroxy-ethanesulfonate, lactobionate, lactate, laurate, lauryl sulfate, malate, maleate, malonate, methanesulfonate, 2- naphthalenesulfonate, nicotinate, nitrate, oleate, oxalate, palmitate, pamoate, pectinate
  • Salts derived from appropriate bases include alkali metal, alkaline earth metal, ammonium and N + (C ⁇ . alkyl) salts.
  • This invention also envisions the quaternization of any basic nitrogen-containing groups of the compounds disclosed herein. Water or oil-soluble or dispersable products may be obtained by such quaternization.
  • Representative alkali or alkaline earth metal salts include sodium, lithium, potassium, calcium, magnesium, and the like.
  • Further pharmaceutically acceptable salts include, when appropriate, nontoxic ammonium, quaternary ammonium, and amine cations formed using counterions such as halide, hydroxide, carboxylate, sulfate, phosphate, nitrate, loweralkyl sulfonate and aryl sulfonate.
  • the pharmaceutically acceptable compositions of the present invention additionally comprise a pharmaceutically acceptable carrier, adjuvant, or vehicle, which, as used herein, includes any and all solvents, diluents, or other liquid vehicle, dispersion or suspension aids, surface active agents, isotonic agents, thickening or emulsifying agents, preservatives, solid binders, lubricants and the like, as suited to the particular dosage form desired.
  • a pharmaceutically acceptable carrier, adjuvant, or vehicle which, as used herein, includes any and all solvents, diluents, or other liquid vehicle, dispersion or suspension aids, surface active agents, isotonic agents, thickening or emulsifying agents, preservatives, solid binders, lubricants and the like, as suited to the particular dosage form desired.
  • Remington's Pharmaceutical Sciences, Sixteenth Edition, E. W. Martin (Mack Publishing Co., Easton, Pa., 1980) discloses various carriers used in formulating pharmaceutically acceptable compositions
  • any conventional carrier medium is incompatible with the compounds of the invention, such as by producing any undesirable biological effect or otherwise interacting in a deleterious manner with any other component(s) of the pharmaceutically acceptable composition, its use is contemplated to be within the scope of this invention.
  • materials which can serve as pharmaceutically acceptable carriers include, but are not limited to, ion exchangers, alumina, aluminum stearate, lecithin, serum proteins, such as human serum albumin, buffer substances such as phosphates, glycine, sorbic acid, or potassium sorbate, partial glyceride mixtures of saturated vegetable fatty acids, water, salts or electrolytes, such as protamine sulfate, disodium hydrogen phosphate, potassium hydrogen phosphate, sodium chloride, zinc salts, colloidal silica, magnesium trisilicate, polyvinyl pyrrolidone, polyacrylates, waxes, polyethylene-polyoxypropylene- block polymers, wool fat, sugars such as lactose, glucose and sucrose; starches such as corn starch and potato starch; cellulose and its derivatives such as sodium carboxymethyl cellulose, ethyl cellulose and cellulose acetate; powdered tragacanth; malt; gelatin; talc
  • a method for the treatment or lessening the severity of a proliferative disorder, a cardiac disorder, a neurodegenerative disorder, a psychotic disorder, an autoimmune disorder, a condition associated with organ transplant, an inflammatory disorder, an immunologically mediated disorder, a viral disease, or a bone disorder comprising administering an effective amount of a compound, or a pharmaceutically acceptable composition comprising a compound to a subject in need thereof.
  • an "effective amount" of the compound or pharmaceutically acceptable composition is that amount effective for treating or lessening the severity of a proliferative disorder, a cardiac disorder, a neurodegenerative disorder, a psychotic disorder, an autoimmune disorder, a condition associated with organ transplant, an inflammatory disorder, an immunologically mediated disorder, a viral disease, or a bone disorder.
  • the compounds and compositions, according to the method of the present invention may be administered using any amount and any route of administration effective for treating or lessening the severity of a proliferative disorder, a cardiac disorder, a neurodegenerative disorder, an autoimmune disorder, a condition associated with organ transplant, an inflammatory disorder, an immunologically mediated disorder, a viral disease, or a bone disorder.
  • the exact amount required will vary from subject to subject, depending on the species, age, and general condition of the subject, the severity of the infection, the particular agent, its mode of administration, and the like.
  • the compounds of the invention are preferably formulated in dosage unit form for ease of administration and uniformity of dosage.
  • dosage unit form refers to a physically discrete unit of agent appropriate for the patient to be treated. It will be understood, however, that the total daily usage of the compounds and compositions of the present invention will be decided by the attending physician within the scope of sound medical judgment.
  • the specific effective dose level for any particular patient or organism will depend upon a variety of factors including the disorder being treated and the severity of the disorder; the activity of the specific compound employed; the specific composition employed; the age, body weight, general health, sex and diet of the patient; the time of administration, route of administration, and rate of excretion of the specific compound employed; the duration of the treatment; drugs used in combination or coincidental with the specific compound employed, and like factors well known in the medical arts.
  • patient means an animal, preferably a mammal, and most preferably a human.
  • compositions of this invention can be administered to humans and other animals orally, rectally, parenterally, intraciste ⁇ ially, intravaginally, intraperitoneally, topically (as by powders, ointments, or drops), bucally, as an oral or nasal spray, or the like, depending on the severity of the infection being treated.
  • the compounds of the invention may be administered orally or parenterally at dosage levels of about 0.01 mg/kg to about 50 mg/kg and preferably from about 1 mg/kg to about 25 mg/kg, of subject body weight per day, one or more times a day, to obtain the desired therapeutic effect.
  • Liquid dosage forms for oral administration include, but are not limited to, pharmaceutically acceptable emulsions, microemulsions, solutions, suspensions, syrups and elixirs.
  • the liquid dosage forms may contain inert diluents commonly used in the art such as, for example, water or other solvents, solubilizing agents and emulsifiers such as ethyl alcohol, isopropyl alcohol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylene glycol, dimethylformamide, oils (in particular, cottonseed, groundnut, corn, germ, olive, castor, and sesame oils), glycerol, tetrahydrofurfuryl alcohol, polyethylene glycols and fatty acid esters of sorbitan, and mixtures thereof.
  • the oral compositions can also include adj
  • Injectable preparations for example, sterile injectable aqueous or oleaginous suspensions may be formulated according to the known art using suitable dispersing or wetting agents and suspending agents.
  • the sterile injectable preparation may also be a sterile injectable solution, suspension or emulsion in a nontoxic parenterally acceptable diluent or solvent, for example, as a solution in 1,3-butanediol.
  • the acceptable vehicles and solvents that may be employed are water, Ringer's solution, U.S. P. and isotonic sodium chloride solution.
  • sterile, fixed oils are conventionally employed as a solvent or suspending medium.
  • any bland fixed oil can be employed including synthetic mono- or diglycerides.
  • fatty acids such as oleic acid are used in the preparation of injectables.
  • the injectable formulations can be sterilized, for example, by filtration through a bacterial-retaining filter, or by incorporating sterilizing agents in the form of sterile solid compositions which can be dissolved or dispersed in sterile water or other sterile injectable medium prior to use.
  • a compound of the present invention In order to prolong the effect of a compound of the present invention, it is often desirable to slow the absorption of the compound from subcutaneous or intramuscular injection. This may be accomplished by the use of a liquid suspension of crystalline or amorphous material with poor water solubility. The rate of absorption of the compound then depends upon its rate of dissolution that, in turn, may depend upon crystal size and crystalline form. Alternatively, delayed absorption of a parenterally administered compound form is accomplished by dissolving or suspending the compound in an oil vehicle. Injectable depot forms are made by forming microencapsule matrices of the compound in biodegradable polymers such as polylactide-polyglycolide.
  • compositions for rectal or vaginal administration are preferably suppositories which can be prepared by mixing the compounds of this invention with suitable non-irritating excipients or carriers such as cocoa butter, polyethylene glycol or a suppository wax which are solid at ambient temperature but liquid at body temperature and therefore melt in the rectum or vaginal cavity and release the active compound.
  • Solid dosage forms for oral administration include capsules, tablets, pills, powders, and granules.
  • the active compound is mixed with at least one inert, pharmaceutically acceptable excipient or carrier such as sodium citrate or dicalcium phosphate and/or a) fillers or extenders such as starches, lactose, sucrose, glucose, mannitol, and silicic acid, b) binders such as, for example, carboxymethylcellulose, alginates, gelatin, polyvinylpyrrolidinone, sucrose, and acacia, c) humectants such as glycerol, d) disintegrating agents such as agar—agar, calcium carbonate, potato or tapioca starch, alginic acid, certain silicates, and sodium carbonate, e) solution retarding agents such as paraffin, f) absorption accelerators such as quaternary ammonium compounds, g) wetting agents such as, for example, cetyl alcohol
  • Solid compositions of a similar type may also be employed as fillers in soft and hard-filled gelatin capsules using such excipients as lactose or milk sugar as well as high molecular weight polyethylene glycols and the like.
  • the solid dosage forms of tablets, dragees, capsules, pills, and granules can be prepared with coatings and shells such as enteric coatings and other coatings well known in the pharmaceutical formulating art. They may optionally contain opacifying agents and can also be of a composition that they release the active ingredient(s) only, or preferentially, in a certain part of the intestinal tract, optionally, in a delayed manner. Examples of embedding compositions that can be used include polymeric substances and waxes. Solid compositions of a similar type may also be employed as fillers in soft and hard-filled gelatin capsules using such excipients as lactose or milk sugar as well as high molecular weight polethylene glycols and the like.
  • the active compounds can also be in micro-encapsulated form with one or more excipients as noted above.
  • the solid dosage forms of tablets, dragees, capsules, pills, and granules can be prepared with coatings and shells such as enteric coatings, release controlling coatings and other coatings well known in the pharmaceutical formulating art.
  • the active compound may be admixed with at least one inert diluent such as sucrose, lactose or starch.
  • Such dosage forms may also comprise, as is normal practice, additional substances other than inert diluents, e.g., tableting lubricants and other tableting aids such a magnesium stearate and microcrystalline cellulose.
  • the dosage forms may also comprise buffering agents. They may optionally contain opacifying agents and can also be of a composition that they release the active ingredient(s) only, or preferentially, in a certain part of the intestinal tract, optionally, in a delayed manner.
  • buffering agents include polymeric substances and waxes.
  • Dosage forms for topical or transdermal administration of a compound of this invention include ointments, pastes, creams, lotions, gels, powders, solutions, sprays, inhalants or patches.
  • the active component is admixed under sterile conditions with a pharmaceutically acceptable carrier and any needed preservatives or buffers as may be required.
  • Ophthalmic formulation, ear drops, and eye drops are also contemplated as being within the scope of this invention.
  • the present invention contemplates the use of transdermal patches, which have the added advantage of providing controlled delivery of a compound to the body.
  • Such dosage forms can be made by dissolving or dispensing the compound in the proper medium.
  • Absorption enhancers can also be used to increase the flux of the compound across the skin.
  • the compounds of the invention are useful as inhibitors of protein kinases.
  • the compounds and compositions of the invention are inhibitors of one or more of JAK-3, JNK-3, CDK-2, SYK, or GSK-3, and thus, without wishing to be bound by any particular theory, the compounds and compositions are particularly useful for treating or lessening the severity of a disease, condition, or disorder where activation of one or more of JAK-3, JNK-3, CDK-2, SYK, or GSK-3 is implicated in the disease, condition, or disorder.
  • the disease, condition, or disorder may also be referred to as "JAK-3, JNK-3, CDK-2, SYK, or GSK-3 -mediated disease" or disease symptom.
  • the present invention provides a method for treating or lessening the severity of a disease, condition, or disorder where activation or one or more of JAK-3, JNK-3, CDK-2, SYK, or GSK-3 is implicated in the disease state.
  • the activity of a compound utilized in this invention as an inhibitor of JAK-3, JNK-3, CDK-2, SYK, or GSK-3 may be assayed in vitro, in vivo or in a cell line.
  • In vitro assays include assays that determine inhibition of either the phosphorylation activity or ATPase activity of activated JAK-3, JNK-3, CDK-2, SYK, or GSK-3. Alternate in vitro assays quantitate the ability of the inhibitor to bind to JAK-3, JNK-3, CDK-2, SYK, or GSK- 3.
  • Inhibitor binding may be measured by radiolabelling the inhibitor prior to binding, isolating the inhibitor/ JAK-3, inhibitor/JNK-3, inhibitor/CDK-2, inhibitor/GSK-3, or inhibitor/SYK complex and determining the amount of radiolabel bound.
  • inhibitor binding may be determined by running a competition experiment where new inhibitors are incubated with JAK-3, JNK-3, CDK-2, SYK, or GSK-3 bound to known radioligands.
  • the term "measurably inhibit”, as used herein means a measurable change in JAK-3, JNK-3, CDK-2, SYK, or GSK-3 activity between a sample comprising said composition and a JAK-3, JNK-3, CDK-2, SYK, or GSK-3 kinase and an equivalent sample comprising JAK-3, JNK-3, CDK-2, SYK, or GSK-3 kinase in the absence of said composition.
  • JAK-mediated disease as used herein means any disease or other deleterious condition in which a JAK family kinase is known to play a role.
  • Such conditions include, without limitation, immune responses such as allergic or type I hypersensitivity reactions, asthma, autoimmune diseases such as transplant rejection, graft versus host disease, rheumatoid arthritis, amyotrophic lateral sclerosis, and multiple sclerosis, neurodegenerative disorders such as Familial amyotrophic lateral sclerosis (FALS), as well as in solid and hematologic malignancies such as leukemias and lymphomas.
  • immune responses such as allergic or type I hypersensitivity reactions, asthma, autoimmune diseases such as transplant rejection, graft versus host disease, rheumatoid arthritis, amyotrophic lateral sclerosis, and multiple sclerosis
  • neurodegenerative disorders such as Familial amyotrophic lateral sclerosis (FALS), as well as in solid and hematologic malignancies such as leukemias and lymphomas.
  • FALS Familial amyotrophic lateral sclerosis
  • the invention provides a method for treating or lessening the severity of a CDK2-mediated disease or condition in a patient comprising the step of administering to said patient a composition according to the present invention.
  • CDK2-mediated disease means any disease or other deleterious condition in which CDK2 is known to play a role. Accordingly, these compounds are useful for treating diseases or conditions that are known to be affected by the activity of CDK2 kinase.
  • Such diseases or conditions include cancer, Alzheimer's disease, restenosis, angiogenesis, glomerulonephritis, cytomegalovirus, HIN, herpes, psoriasis, atherosclerosis, alopecia, and autoimmune diseases such as rheumatoid arthritis, viral infections, neurodegenerative disorders, disorders associated with thymocyte apoptosis, or proliferative disorders resulting from the deregulation of the cell cycle, especially of the progression from Gi to S phase.
  • the invention provides a method for treating or lessening the severity of a CDK2 -mediated disease or condition in a patient comprising the step of administering to said patient a composition according to the present invention.
  • J ⁇ K-mediated condition means any disease or other deleterious condition in which J ⁇ K is known to play a role.
  • Such conditions include, without limitation, inflammatory diseases, autoimmune diseases, destructive bone disorders, proliferative disorders, cancer, infectious diseases, neurodegenerative diseases, allergies, reperfusion/ischemia in stroke, heart attacks, angiogenic disorders, organ hypoxia, vascular hyperplasia, cardiac hypertrophy, thrombin-induced platelet aggregation, and conditions associated with prostaglandin endoperoxidase synthase-2.
  • J ⁇ K-mediated conditions also include ischemia/reperfusion in stroke, heart attacks, myocardial ischemia, organ hypoxia, vascular hyperplasia, cardiac hypertrophy, hepatic ischemia, liver disease, congestive heart failure, pathologic immune responses such as that caused by T cell activation and thrombin-induced platelet aggregation.
  • JNK inhibitors of the instant invention may be capable of inhibiting the expression of inducible pro-inflammatory proteins. Therefore, other "JNK-mediated conditions" which may be treated by the compounds of this invention include edema, analgesia, fever and pain, such as neuromuscular pain, headache, cancer pain, dental pain and arthritis pain.
  • the invention provides a method for treating or lessening the severity of a GSK3 -mediated disease or condition in a patient comprising the step of administering to said patient a composition according to the present invention.
  • GSK3 -mediated disease or "GSK3 -mediated condition”, as used herein, means any disease or other deleterious condition in which GSK3 protein kinase is known to play a role. Such conditions include, without limitation, diabetes, neurodegenerative disorders, Alzheimer's disease, Huntington's, Parkinson's, AIDS associated dementia, amyotrophic lateral sclerosis (AML), multiple sclerosis (MS), schizophrenia, stroke, cardiomycete hypertrophy, and baldness.
  • AML amyotrophic lateral sclerosis
  • MS multiple sclerosis
  • the invention provides a method for treating or lessening the severity of a Syk-mediated disease or condition in a patient comprising the step of administering to said patient a composition according to the present invention.
  • Syk-mediated disease or "Syk-mediated condition”, as used herein, means any disease or other deleterious condition in which Syk protein kinase is known to play a role. Such conditions include, without limitation, allergic disorders, especially asthma.
  • the compounds and pharmaceutically acceptable compositions of the present invention can be employed in combination therapies, that is, the compounds and pharmaceutically acceptable compositions can be administered concurrently with, prior to, or subsequent to, one or more other desired therapeutics or medical procedures.
  • the particular combination of therapies (therapeutics or procedures) to employ in a combination regimen will take into account compatibility of the desired therapeutics and/or procedures and the desired therapeutic effect to be achieved. It will also be appreciated that the therapies employed may achieve a desired effect for the same disorder (for example, an inventive compound may be administered concurrently with another agent used to treat the same disorder), or they may achieve different effects (e.g., control of any adverse effects). As used herein, additional therapeutic agents that are normally administered to treat or prevent a particular disease, or condition, are known as "appropriate for the disease, or condition, being treated”. [00141] For example, chemotherapeutic agents or other anti-proliferative agents may be combined with the compounds of this invention to treat proliferative diseases and cancer.
  • chemotherapeutic agents include, but are not limited to,
  • other therapies or anticancer agents that may be used in combination with the inventive anticancer agents of the present invention include surgery, radiotherapy (in but a few examples, gamma.-radiation, neutron beam radiotherapy, electron beam radiotherapy, proton therapy, brachytherapy, and systemic radioactive isotopes, to name a few), endocrine therapy, biologic response modifiers (interferons, interleukins, and tumor necrosis factor (TNF) to name a few), hyperthermia and cryotherapy, agents to attenuate any adverse effects (e.g., antiemetics), and other approved chemotherapeutic drugs, including, but not limited to, alkylating drugs (mechlorethamine, chlorambucil, Cyclophosphamide, Melphalan, Ifosfamide), antimetabolites (Methotrexate), purine antagonists and pyrimidine antagonists (6-Mercaptopurine, 5-Fl
  • agents the inhibitors of this invention may also be combined with include, without limitation: treatments for Alzheimer's Disease such as Aricept and Excelon ® ; treatments for Parkinson's Disease such as L-DOPA/carbidopa, entacapone, ropinrole, pramipexole, bromocriptine, pergolide, trihexephendyl, and amantadine; agents for treating Multiple Sclerosis (MS) such as beta interferon (e.g., Avonex ® and Rebif ® ), Copaxone ® , and mitoxantrone; treatments for asthma such as albuterol and Singulair ® ; agents for treating schizophrenia such as zyprexa, risperdal, seroquel, and haloperidol; anti- inflammatory agents such as corticosteroids, T ⁇ F blockers, IL-1 RA, azathioprine, cyclophosphamide, and sulfasalazine; immunomodulates
  • the amount of additional therapeutic agent present in the compositions of this invention will be no more than the amount that would normally be administered in a composition comprising that therapeutic agent as the only active agent.
  • the amount of additional therapeutic agent in the presently disclosed compositions will range from about 50% to 100% of the amount normally present in a composition comprising that agent as the only therapeutically active agent.
  • the present invention in another aspect, includes a composition for coating an implantable device comprising a compound of the present invention as described generally above, and in classes and subclasses herein, and a carrier suitable for coating said implantable device.
  • the present invention includes an implantable device coated with a composition comprising a compound of the present invention as described generally above, and in classes and subclasses herein, and a carrier suitable for coating said implantable device.
  • Vascular stents for example, have been used to overcome restenosis (re- narrowing of the vessel wall after injury).
  • patients using stents or other implantable devices risk clot formation or platelet activation.
  • These unwanted effects may be prevented or mitigated by pre-coating the device with a pharmaceutically acceptable composition comprising a kinase inhibitor.
  • Suitable coatings and the general preparation of coated implantable devices are described in US Patents 6,099,562; 5,886,026; and 5,304,121.
  • the coatings are typically biocompatible polymeric materials such as a hydrogel polymer, polymethyldisiloxane, polycaprolactone, polyethylene glycol, polylactic acid, ethylene vinyl acetate, and mixtures thereof.
  • the coatings may optionally be further covered by a suitable topcoat of fluorosilicone, polysaccarides, polyethylene glycol, phospholipids or combinations thereof to impart controlled release characteristics in the composition.
  • Another aspect of the invention relates to inhibiting JAK-3, JNK-3, CDK-2, SYK, or GSK-3 activity in a biological sample or a patient, which method comprises administering to the patient, or contacting said biological sample with a compound of formula I or a composition comprising said compound.
  • biological sample includes, without limitation, cell cultures or extracts thereof; biopsied material obtained from a mammal or extracts thereof; and blood, saliva, urine, feces, semen, tears, or other body fluids or extracts thereof.
  • Inhibition of JAK-3, JNK-3, CDK-2, SYK, or GSK-3 kinase activity in a biological sample is useful for a variety of purposes that are known to one of skill in the art. Examples of such purposes include, but are not limited to, blood transfusion, organ- transplantation, biological specimen storage, and biological assays.
  • Scheme I above depicts the synthesis of several exemplary compounds.
  • the examples below describe general procedures for the preparation of compounds herein (as shown in Schemes 1, 2 and 3 above) and Table 3 depicts characterization for exemplary compounds of the invention.
  • Procedure A general procedure for the synthesis of guanidines
  • Procedure B general procedure for the synthesis of guanidines
  • the tube was sealed and heated to 60C overnight with magnetic stirring.
  • the reaction was concentrated to dryness, basified with 2N NaOH, and extracted with methylene chloride
  • Procedure B (modified): general procedure for the synthesis of guanidines
  • Procedure D procedure for the synthesis of N-methylated benzoxazin eneaminones
  • Procedure E general procedure for the synthesis of N-alkylated benzoxazin acetophenones
  • the crude was purified via flash chromatography on silica gel, and eluted with ether or ethyl acetate).
  • Procedure F general procedure for the synthesis of eneaminones
  • N,N-dimethylformamide or tetrahydrofuran may be used as solvent
  • tert-Butoxybis (dimethylamino) methane Bredereck's reagent, 35mmol, 1.75 eq.
  • the reaction was heated to reflux overnight. Upon concentration a precipitate forms, which was filtered, and used directly.
  • the crude may be purified via flash chromatography on silica gel, eluted with ethyl acetate/hexane or acetone/hexane.
  • Procedure H general procedure for the synthesis of phenylaminopyrimidines
  • Procedure J general procedure for the synthesis of phenylaminopyrimidines
  • Procedure J (modified): general procedure for the synthesis of phenylaminopyrimidines
  • Procedure K general procedure for the preparation of phenylaminopyrimidines
  • Procedure L general procedure for the reduction of nitrobenzenes to anilines
  • Procedure M general procedure for amide couplings from carboxylic acids
  • the carboxylic acid 100 to 200umol
  • EDC 110 to 400umol, 1.1 to 2 equivalents
  • HOBT 40 to 200umol, 0.4 to 1 equivalent
  • DMF 1 to 3ml
  • N-methylmorpholine 250 to 500ul
  • desired amine or aminehydrochloride salt 300 to 600umol, 3 to 6 equivalents
  • the reaction was sealed and heated to 122C, overnight.
  • the reaction was then concentrated to dryness and extracted with ethyl acetate/water.
  • the organic layer was dried over sodium sulfate, filtered and concentrated to give crude product.
  • the product was purified via silica gel chromatography eluted with ethyl acetate/hexanes, ethyl acetate or acetone.
  • Procedure N general procedure for hydrolysis of esters
  • the plates were then washed with PBST, 100 ⁇ L HRP-Conjugated 4G10 antibody was added, and the plate incubated for 90 minutes at 30°C.
  • the plate was again washed with PBST, 100 ⁇ L TMB solution is added, and the plates were incubated for another 30 minutes at 30°C.
  • Sulfuric acid 100 ⁇ L of IM was added to stop the reaction and the plate is read at 450 nm to obtain the optical densities for analysis to determine Ki values.
  • An assay stock buffer solution was prepared containing all of the reagents listed above, with the exception of CDK-2/Cyclin A, DTT and the test compound of interest.
  • 56 ⁇ l of the test reaction was placed in a 384 well plate followed by addition of 1 ⁇ l of 2 mM DMSO stock containing the test compound (final compound concentration 30 ⁇ M).
  • the plate was preincubated for -10 minutes at 30 °C and the reaction initiated by addition of 10 ⁇ l of enzyme (final concentration 25 nM). Rates of reaction were obtained using a BioRad Ultramark plate reader (Hercules, CA) over a 5 minute read time at 30°C and KiS were determined using standard methods.
  • the compound numbers correspond to the compound numbers in Table 1 and compounds were tested and found to inhibit CDK-2 Certain compounds described herein were shown to have KjS less than 0.1 micromolar ( ⁇ M).
  • the reaction was initiated by the addition of 10 ⁇ M ATP and the assay plate is inserted into the spectrophotometer's assay plate compartment that was maintained at 30°C.
  • the decrease of absorbance at 340 nm was monitored as a function of time.
  • the rate data as a function of inhibitor concentration was fitted to competitive inhibition kinetic model to determine the Kj.
  • the compound numbers correspond to the compound numbers in Table 1 and compounds were tested and found to inhibit JNK-3. Certain compounds described herein were shown to have KjS less than 0.1 micromolar ( ⁇ M).
  • the compound numbers correspond to the compound numbers in Table 1 and were found to inhibit Syk. Certain compounds described herein were shown to have KjS less than 0.1 micromolar ( ⁇ M).
  • An assay stock buffer solution was prepared containing all of the reagents listed above with the exception of ATP and the test compound of interest. 59 ⁇ l of the test reaction was placed in a 96 well H-diameter plate (Corning, Coming, NY) then treated with 1 ⁇ l of a 2 mM DMSO stock containing the test compound (final compound concentration 30 ⁇ M). The plate was incubated for ⁇ 10 minutes at 30 °C then the reaction initiated by addition of 7 ⁇ l of ATP (final concentration 10 ⁇ M). Rates of reaction were obtained using a Molecular Devices Spectramax plate reader (Sunnyvale, CA) over a 5 minute read time at 30 °C, and Kj values were determined using standard methods.
  • the compound numbers correspond to the compound numbers in Table 1 and were found to inhibit GSK-3. Certain compounds described herein were shown to have KiS less than 0.1 micromolar ( ⁇ M).

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Abstract

The present invention provides a compound of formula I: or a pharmaceutically acceptable salt thereof. The invention also provides pharmaceutically acceptable compositions comprising the compounds of the invention and methods of utilizing those compounds and compositions in the treatment of various protein kinase mediated disorders.

Description

COMPOUNDS USEFUL AS INHIBITORS OF JAK ND OTHER PROTEIN KINASES
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] The present application claims priority under 35 U.S.C. § 119(e) to U.S. Provisional Application number 60/424,043, filed November 5, 2002, entitled "Compositions Useful as Inhibitors of Jak and Other Protein Kinases, the entire contents of which is hereby incorporated by reference.
TECHNICAL FIELD OF INVENTION [0002] The present invention relates to compounds useful as inhibitors of protein kinases. The invention also provides pharmaceutically acceptable compositions comprising the compounds of the invention and methods of using the compositions in the treatment of various disorders.
BACKGROUND OF THE INVENTION [0003] The search for new therapeutic agents has been greatly aided in recent years by a better understanding of the structure of enzymes and other biomolecules associated with diseases. One important class of enzymes that has been the subject of extensive study is protein kinases.
[0004] Protein kinases constitute a large family of structurally related enzymes that are responsible for the control of a variety of signal transduction processes within the cell. (See, Hardie, G. and Hanks, S. The Protein Kinase Facts Book, I and II, Academic Press, San Diego, CA: 1995). Protein kinases are thought to have evolved from a common ancestral gene due to the conservation of their structure and catalytic function. Almost all kinases contain a similar 250-300 amino acid catalytic domain. The kinases may be categorized into families by the substrates they phosphorylate (e.g., protein-tyrosine, protein-serine/threonine, lipids, etc.). Sequence motifs have been identified that generally correspond to each of these kinase families (See, for example, Hanks, S.K., Hunter, TΛ, FASEB J. 1995, 9, 576-596; Knighton et al, Science 1991, 253, 407-414; Hiles et al, Cell 1992, 70, 419-429; Kunz et al, Cell 1993, 73, 585-596; Garcia-Bustos et al, EMBOJ. 1994, 13, 2352-2361). [0005] Many diseases are associated with abnormal cellular responses triggered by protein kinase-mediated events. These diseases include autoimmune diseases, inflammatory diseases, bone diseases, metabolic diseases, neurological and neurodegenerative diseases, cancer, cardiovascular diseases, allergies and asthma, Alzheimer's disease and hormone- related diseases. Accordingly, there has been a substantial effort in medicinal chemistry to find protein kinase inhibitors that are effective as therapeutic agents.
[0006] The Janus kinases (JAK) are a family of tyrosine kinases consisting of JAK1, JAK2, JAK3 and TYK2. The JAKs play a critical role in cytokine signaling. The downstream substrates of the JAK family of kinases include the signal transducer and activator of transcription (STAT) proteins. JAK/STAT signaling has been implicated in the mediation of many abnormal immune responses such as allergies, asthma, autoimmune diseases such as transplant rejection, rheumatoid arthritis, amyotrophic lateral sclerosis and multiple sclerosis as well as in solid and hematologic malignancies such as leukemias and lymphomas. The pharmaceutical intervention in the JAK/STAT pathway has been reviewed [Frank Mol. Med. 5, 432-456 (1999) & Seidel, et al, Oncogene 19, 2645-2656 (2000)].
[0007] JAK1, JAK2, and TYK2 are ubiquitously expressed, while JAK3 is predominantly expressed in hematopoietic cells. JAK3 binds exclusively to the common cytokine receptor gamma chain (γc) and is activated by IL-2, IL-4, IL-7, IL-9, and IL-15. The proliferation and survival of murine mast cells induced by IL-4 and IL-9 have, in fact, been shown to be dependent on JAK3- and γc- signaling [Suzuki et al, Blood 96, 2172-2180 (2000)].
[0008] Cross-linking of the high-affinity immunoglobulin (Ig) E receptors of sensitized mast cells leads to a release of proinflammatory mediators, including a number of vasoactive cytokines resulting in acute allergic, or immediate (type I) hypersensitivity reactions [Gordon et al, Nature 346, 274-276 (1990) & Galli, N. Engl J. Med., 328, 257-265 (1993)]. A crucial role for JAK3 in IgE receptor-mediated mast cell responses in vitro and in vivo has been established [Malaviya, et al, Biochem. Biophys. Res. Commun. 257, 807-813 (1999)]. In addition, the prevention of type I hypersensitivity reactions, including anaphylaxis, mediated by mast cell-activation through inhibition of JAK3 has also been reported [Malaviya et al, J. Biol. Chem. 274,27028-27038 (1999)]. Targeting mast cells with JAK3 inhibitors modulated mast cell degranulation in vitro and prevented IgE receptor/antigen-mediated anaphylactic reactions in vivo. [0009] A recent study described the successful targeting of JAK3 for immune suppression and allografl acceptance. The study demonstrated a dose-dependent survival of Buffalo heart allografl in Wistar Furth recipients upon administration of inhibitors of JAK3 indicating the possibility of regulating unwanted immune responses in graft versus host disease [Kirken, Transpl Proc. 33, 3268-3270 (2001)].
[0010] IL-4-mediated STAT-phosphorylation has been implicated as the mechanism involved in early and late stages of rheumatoid arthritis (RA). Up-regulation of proinflammatory cytokines in RA synovium and synovial fluid is a characteristic of the disease. It has been demostrated that IL-4— mediated activation of IL-4/STAT pathway is mediated through the Janus Kinases (JAK 1 & 3) and that IL-4-associated JAK kinases are expressed in the RA synovium [Muller-Ladner, et al, J. Immunol 164, 3894-3901 (2000)]. [0011] Familial amyotrophic lateral sclerosis (FALS) is a fatal neurodegenerative disorder affecting about 10% of ALS patients. The survival rates of FALS mice were increased upon treatment with a JAK3 specific inhibitor. This suggested that JAK3 plays a role in FALS [Trieu, et al, Biochem. Biophys. Res. Commun. 267, 22-25 (2000)]. [0012] Signal transducer and activator of transcription (STAT) proteins are activated by, among others, the JAK family kinases. Results form a recent study suggested the possibility of intervention in the JAK/STAT signaling pathway by targeting JAK family kinases with specific inhibitors for the treatment of leukemia [Sudbeck, et al, Clin. Cancer Res. 5, 1569- 1582 (1999)]. JAK3 specific compounds were shown to inhibit the clonogenic growth of JAK3-expressing cell lines DAUDI, RAMOS, LC1;19, NALM-6, MOLT-3 and HL-60. [0013] In animal models, TEL/JAK2 fusion proteins have induced myeloproliferative disorders and in hematopoietic cell lines, introduction of TEL/JAK2 resulted in activation of STAT1, STAT3, STAT5, and cytokine-independent growth [Schwaller, et al, EMBO J. 17, 5321-5333 (1998)].
[0014] Inhibition of JAK 3 and TYK 2 abrogated tyrosine phosphorylation of STAT3, and inhibited cell growth of mycosis fungoides, a form of cutaneous T cell lymphoma. These results implicated JAK family kinases in the constitutively activated JAK/STAT pathway that is present in mycosis fungoides [Nielsen, et al, Proc. Nat. Acad. Sci. U.S.A. 94, 6764-6769 (1997)]. Similarly, STAT3, STAT5, JAK1 and JAK2 were demonstrated to be constitutively activated in mouse T cell lymphoma characterized initially by LCK over-expression, thus further implicating the JAK/STAT pathway in abnormal cell growth [Yu, et al, J. Immunol. 159, 5206-5210 (1997)]. In addition, IL-6 -mediated STAT3 activation was blocked by an inhibitor of JAK, leading to sensitization of myeloma cells to apoptosis [Catlett-Falcone, et al, Immunity 10 , 105-115 (1999)].
[0015] Glycogen synthase kinase-3 (GSK-3) is a serine/threonine protein kinase comprised of and β isoforms that are each encoded by distinct genes [Coghlan et al., Chemistry & Biology, 7, 793-803 (2000); Kim and Kimmel, Curr. Opinion Genetics Dev., 10, 508-514 (2000)]. GSK-3 has been implicated in various diseases including diabetes, Alzheimer's disease, CNS disorders such as manic depressive disorder and neurodegenerative diseases, and cardiomyocete hypertrophy [WO 99/65897; WO 00/38675; and Haq et al., J. Cell Biol. (2000) 151, 117]. These diseases may be caused by, or result in, the abnormal operation of certain cell signaling pathways in which GSK-3 plays a role. GSK-3 has been found to phosphorylate and modulate the activity of a number of regulatory proteins. These include glycogen synthase which is the rate limiting enzyme necessary for glycogen synthesis, the microtubule associated protein Tau, the gene transcription factor β- catenin, the translation initiation factor elF2B, as well as ATP citrate lyase, axin, heat shock factor- 1, c-Jun, c-Myc, c-Myb, CREB, and CEPBα. These diverse targets implicate GSK-3 in many aspects of cellular metabolism, proliferation, differentiation and development. [0016] In a GSK-3 mediated pathway that is relevant for the treatment of type II diabetes, insulin-induced signaling leads to cellular glucose uptake and glycogen synthesis. Along this pathway, GSK-3 is a negative regulator of the insulin-induced signal. Normally, the presence of insulin causes inhibition of GSK-3 mediated phosphorylation and deactivation of glycogen synthase. The inhibition of GSK-3 leads to increased glycogen synthesis and glucose uptake [Klein et al., PNAS, 93, 8455-9 (1996); Cross et al., Biochem. J., 303, 21-26 (1994); Cohen, Biochem. Soc. Trans., 21, 555-567 (1993); Massillon et al, Biochem J. 299, 123-128 (1994)]. However, in a diabetic patient where the insulin response is impaired, glycogen synthesis and glucose uptake fail to increase despite the presence of relatively high blood levels of insulin. This leads to abnormally high blood levels of glucose with acute and long term effects that may ultimately result in cardiovascular disease, renal failure and blindness. In such patients, the normal insulin-induced inhibition of GSK-3 fails to occur. It has also been reported that in patients with type II diabetes, GSK-3 is overexpressed [WO 00/38675]. Therapeutic inhibitors of GSK-3 are therefore potentially useful for treating diabetic patients suffering from an impaired response to insulin.
[0017] GSK-3 activity has also been associated with Alzheimer's disease. This disease is characterized by the well-known β-amyloid peptide and the formation of intracellular neurofibrillary tangles. The neurofibrillary tangles contain hyperphosphorylated Tau protein where Tau is phosphorylated on abnormal sites. GSK-3 has been shown to phosphorylate these abnormal sites in cell and animal models. Furthermore, inhibition of GSK-3 has been shown to prevent hyperphosphorylation of Tau in cells [Lovestone et al., Current Biology 4, 1077-86 (1994); Brownlees et al., Neuroreport 8, 3251-55 (1997)]. Therefore, it is believed that GSK-3 activity may promote generation of the neurofibrillary tangles and the progression of Alzheimer's disease.
[0018] Another substrate of GSK-3 is β-catenin which is degradated after phosphorylation by GSK-3. Reduced levels of β-catenin have been reported in schizophrenic patients and have also been associated with other diseases related to increase in neuronal cell death [Zhong et al., Nature, 395, 698-702 (1998); Takashima et al., PNAS, 90, 7789-93 (1993); Pei et al., J. Neuropathol Exp, 56, 70-78 (1997)].
[0019] Syk is a tyrosine kinase that plays a critical role in FcεRI mediated mast cell degranulation and eosiniphil activation. Accordingly, Syk kinase is implicated in various allergic disorders, in particular asthma.It has been shown that that Syk binds to the phosphorylated gamma chain of the FcεRI receptor via N-terminal SH2 domains and is essential for downstream signaling [Taylor et al, Mol Cell Biol 1995; 15:4149. [0020] Inhibition of eosinophil apoptosis has been proposed as key mechanisms for the development of blood and tissue eosinophilia in asthma. IL-5 and GM-CSF are upregulated in asthma and are proposed to cause blood and tissue eosinophilia by inhibition of eosinophil apoptosis. Inhibition of eosinophil apoptosis has been proposed as a key mechanism for the development of blood and tissue eosinophilia in asthma. It has been reported that Syk kinase is required for the prevention of eosinophil apoptosis by cytokines (using antisense) [Yousefi et al, J Exp Med 1996; 183: 1407].
[0021] The role of Syk in FcγR dependent and independent response in bone marrow derived macrophages has been determined by using irradiated mouse chimeras reconstituted with fetal liver cells from Syk -/- embryos. Syk deficient macrophages were defective in phagocytosis induced by FcγR but showed normal phagocytosis in response to complement [Kiefer et al, Mol Cell Biol 1998; 18:4209]. It has also been reported that aerosolized Syk antisense suppresses Syk expression and mediator release from macrophages [Stenton et al, J Immunology 2000; 164: 3790].
[0022] Cyclin-dependent kinases (CDKs) are serine/threonine protein kinases consisting of a β-sheet rich amino-terminal lobe and a larger carboxy-terminal lobe which is largely α- helical. The CDKs display the 11 subdomains shared by all protein kinases and range in molecular mass from 33 to 44 kD. This family of kinases, which includes CDK1, CKD2, CDK4, and CDK6, requires phosphorylation at the residue corresponding to CDK2 Thrl60 in order to be fully active [Meijer, L., Drug Resistance Updates, 3, 83-88 (2000)]. [0023] Each CDK complex is formed from a regulatory cyclin subunit (e.g., cyclin A, Bl, B2, Dl, D2, D3, and E) and a catalytic kinase subunit (e.g., CDK1, CDK2, CDK4, CDK5, and CDK6). Each different kinase/cyclin pair functions to regulate the different and specific phases of the cell cycle known as the Gl, S, G2, and M phases [Nigg, E., Nature Reviews, 2, 21-32 (2001); Flatt, P., Pietenpol, J., Drug Metabolism Reviews, 32, 283-305 (2000)]. [0024] The CDKs have been implicated in cell proliferation disorders, particularly in cancer. Cell proliferation is a result of the direct or indirect deregulation of the cell division cycle and the CDKs play a critical role in the regulation of the various phases of this cycle. For example, the over-expression of cyclin Dl is commonly associated with numerous human cancers including breast, colon, hepatocellular carcinomas and gliomas [Flatt, P., Pietenpol, J., Drug Metabolism Reviews, 32, 283-305 (2000)]. The CDK2/cyclin E complex plays, a key role in the progression from the early Gi to S phases of the cell cycle and the overexpression of cyclin E has been associated with various solid tumors. Therefore, inhibitors of cyclins Dl, E, or their associated CDKs are useful targets for cancer therapy [Kaubisch, A., Schwartz, G., The Cancer Journal, 6, 192-212 (2000)]. [0025] CDKs, especially CDK2, also play a role in apoptosis and T-cell development. CDK2 has been identified as a key regulator of thymocyte apoptosis [Williams, O., et al, European Journal of Immunology, 709-713 (2000)]. Stimulation of CDK2 kinase activity is associated with the progression of apoptosis in thymocytes, in response to specific stimuli. Inhibition of CDK2 kinase activity blocks this apoptosis resulting in the protection of thymocytes.
[0026] In addition to regulating the cell cycle and apoptosis, the CDKs are directly involved in the process of transcription. Numerous viruses require CDKs for their replication process. Examples where CDK inhibitors restrain viral replication include human cytomegakovirus, herpes virus, and varicella-zoster virus [Meijer, L., Drug Resistance Updates, 3, 83-88 (2000)].
[0027] Inhibition of CDK is also useful for the treatment of neurodegenerative disorders such as Alzheimer's disease. The appearance of Paired Helical Filaments (PHF), associated with Alzheimer's disease, is caused by the hyperphosphorylation of Tau protein by CDK5/p25 [Meijer, L., Drug Resistance Updates, 3, 83-88 (2000)]. [0028] JNK is a member of the mitogen-activated protein (MAP) kinase family. MAP kinases (MAPKs) are activated by a variety of signals including growth factors, cytokines, UN radiation, and stress-inducing agents. MAPKs are serine/threonine kinases and their activation occur by dual phosphorylation of threonine and tyrosine at the Thr-X-Tyr segment in the activation loop. MAPKs phosphorylate various substrates including transcription factors, which in turn regulate the expression of specific sets of genes and thus mediate a specific response to the stimulus.
[0029] Three distinct genes, JΝK1, JΝK2, JNK3 have been identified for this kinase family and at least ten different splicing isoforms of JNKs exist in mammalian cells [Gupta et al, EMBO J., 15, 2760-70 (1996)]. Members of the JNK family are activated by proinflammatory cytokines, such as tumor necrosis factor-α (TNFα) and interleukin-1 β (IL- lβ), as well as by environmental stress, including anisomycin, UN irradiation, hypoxia, and osmotic shock [Minden et al., Biochemica et Biophysica Acta, 1333, F85-F104 (1997)]. [0030] The down-stream substrates of JΝKs include transcription factors c-Jun, ATF-2, Elkl, p53 and a cell death domain protein (DEΝΝ) [Zhang et al. Proc. Natl. Acad. Sci. USA, 95, 2586-91 (1998)]. Each JΝK isoform binds to these substrates with different affinities, suggesting a regulation of signaling pathways by substrate specificity of different JΝKs in vivo (Gupta et al., supra).
[0031] JΝKs, along with other MAPKs, have been implicated in having a role in mediating cellular response to cancer, thrombin-induced platelet aggregation, immunodeficiency disorders, autoimmune diseases, cell death, allergies, osteoporosis and heart disease. The therapeutic targets related to activation of the JΝK pathway include chronic myelogenous leukemia (CML), rheumatoid arthritis, asthma, osteoarthritis, ischemia, cancer and neurodegenerative diseases.
[0032] Several reports have detailed the importance of JΝK activation associated with liver disease or episodes of hepatic ischemia [Nat. Genet. 21, 326-9 (1999); FEBS Lett 420, 201-4 (1997); J. Clin. Invest. 102, 1942-50 (1998); Hepatology 28, 1022-30 (1998)]. Therefore, inhibitors of JΝK may be useful to treat various hepatic disorders. [0033] A role for JΝK in cardiovascular disease such as myocardial infarction or congestive heart failure has also been reported as it has been shown JΝK mediates hypertrophic responses to various forms of cardiac stress [Circ. Res. 83, 167-78 (1998); Circulation 97, 1731-7 (1998); J Biol Chem. 272, 28050-6 (1997); Circ. Res. 79, 162-73 (1996); Circ. Res. 78, 947-53 (1996); J. Clin. Invest. 97, 508-14 (1996)]. [0034] It has been demonstrated that the JNK cascade also plays a role in T-cell activation, including activation of the IL-2 promoter. Thus, inhibitors of JNK may have therapeutic value in altering pathologic immune responses [J. Immunol 162, 3176-87 (1999); Eur. J. Immunol. 28, 3867-77 (1998); J. Exp. Med. 186, 941-53 (1997); Eur. J. Immunol 26, 989-94 (1996)].
[0035] A role for JNK activation in various cancers has also been established, suggesting the potential use of JNK inhibitors in cancer. For example, constitutively activated JNK is associated with HTLN-1 mediated tumorigenesis [Oncogene 13, 135-42 (1996)]. JΝK may play a role in Kaposi's sarcoma (KS) because it is thought that the proliferative effects of βFGF and OSM on KS cells are mediated by their activation of the JΝK signaling pathway [J. Clin. Invest. 99, 1798-804 (1997)]. Other proliferative effects of other cytokines implicated in KS proliferation, such as vascular endothelial growth factor (VΕGF), IL-6 and TΝFα, may also be mediated by JΝK. In addition, regulation of the c-jun gene in p210 BCR- ABL transformed cells corresponds with activity of JΝK, suggesting a role for JΝK inhibitors in the treatment for chronic myelogenous leukemia (CML) [Blood 92, 2450-60 (1998)]. [0036] JΝK1 and JΝK2 are widely expressed in a variety of tissues. In contrast, JNK3, is selectively expressed in the brain and to a lesser extent in the heart and testis [Gupta et al., supra; Mohit et al., Neuron 14, 67-78 (1995); Martin et al., Brain Res. Mol. Brain Res. 35, 47-57 (1996)]. JNK3 has been linked to neuronal apoptosis induced by kainic acid, indicating a role of JNK in the pathogenesis of glutamate neurotoxicity. In the adult human brain, JNK3 expression is localized to a subpopulation of pyramidal neurons in the CA1, CA4 and subiculum regions of the hippocampus and layers 3 and 5 of the neocortex [Mohit et al., supra]. The CA1 neurons of patients with acute hypoxia showed strong nuclear JNK3- immunoreactivity compared to minimal, diffuse cytoplasmic staining of the hippocampal neurons from brain tissues of normal patients [Zhang et al., supra]. Thus, JNK3 appears to be involved involved in hypoxic and ischemic damage of CA1 neurons in the hippocampus. [0037] In addition, JNK3 co-localizes immunochemically with neurons vulnerable in Alzheimer's disease [Mohit et al., supra]. Disruption of the JNK3 gene caused resistance of mice to the excitotoxic glutamate receptor agonist kainic acid, including the effects on seizure activity, AP-1 transcriptional activity and apoptosis of hippocampal neurons, indicating that the JNK3 signaling pathway is a critical component in the pathogenesis of glutamate neurotoxicity (Yang et al., Nature, 389, ,865-870 (1997)]. [0038] Based on these findings, JNK signalling, especially that of JNK3, has been implicated in the areas of apoptosis-driven neurodegenerative diseases such as Alzheimer's Disease, Parkinson's Disease, ALS (Amyotrophic Lateral Sclerosis), epilepsy and seizures, Huntington's Disease, traumatic brain injuries, as well as ischemic and hemorrhaging stroke. [0039] Accordingly, there is a great need to develop inhibitors of JAK, JNK, GSK, SYK, , and CDK protein kinases that are useful in treating various diseases or conditions associated with JAK, JNK, GSK, SYK, and CDK activation, particularly given the inadequate treatments currently available for the majority of these disorders.
SUMMARY OF THE INVENTION
[0040] It has now been found that compounds of this invention, and pharmaceutically acceptable compositions thereof, are effective as inhibitors of JAK, JNK, GSK, SYK, and
CDK protein kinases. In certain embodiments, these compounds are effective as inhibitors of
JAK-3, JNK-3, GSK-3, SYK, and CDK-2 protein kinases. These compounds have the general formula I:
Figure imgf000010_0001
I
[0041] or a pharmaceutically acceptable derivative thereof, wherein R1, T, Rx, Z1, Z2, Z3, Z4, Z5, Z6, and Z7 are as defined below.
[0042] These compounds and pharmaceutical compositions thereof are useful for treating or preventing a variety of disorders, such as heart disease, diabetes, Alzheimer's disease, immunodeficiency disorders, inflammatory diseases, allergic diseases, autoimmune diseases, destructive bone disorders such as osteoporosis, proliferative disorders, infectious diseases, immunologically-mediated diseases, and viral diseases. The compositions are also useful in methods for preventing cell death and hyperplasia and therefore may be used to treat or prevent reperfusion/ischemia in stroke, heart attacks, and organ hypoxia. The compositions are also useful in methods for preventing thrombin-induced platelet aggregation. The compositions are especially useful for disorders such as chronic myelogenous leukemia (CML), rheumatoid arthritis, asthma, osteoarthritis, ischemia, cancer, liver disease including hepatic ischemia, heart disease such as myocardial infarction and congestive heart failure, pathologic immune conditions involving T cell activation, and neurodegenerative disorders.
DETAILED DESCRIPTION OF THE INVENTION
[0043] I. General Description of Compounds of the Invention: [0044] The present invention relates to a compound of formula I:
Figure imgf000011_0001
or a pharmaceutically acceptable salt thereof, wherein:
R1 is Q-Ar1, wherein Q is a bond or is a Cι. alkylidene chain wherein one methylene unit of Q is optionally replaced by O, NR, NRCO, NRCONR, NRCO2, CO, CO2, CONR, OCONR, SO2, SO2NR, NRSO2, NRSO2NR, COCO, or COCH2CO;
Ar1 is a 5-7 membered saturated, partially unsaturated, or fully unsaturated monocyclic ring having 0-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or an 8-12 membered saturated, partially unsaturated, or fully unsaturated bicyclic ring system having 0-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; wherein Ar1 is optionally substituted with q independent occurrences of Z-R ; wherein q is 0-5, Z is a bond or is a -Cό alkylidene chain wherein up to two methylene units of Z are optionally and independently replaced by CO, CO2, COCO, CONR, OCONR, NRNR, NRNRCO, NRCO, NRCO2, NRCONR, SO, SO2, NRSO2, SO2NR, NRSO2NR, O, S, or NR; and each occurrence of R is independently selected from R', halogen, NO2, CN, OR', SR', N(R')2, NR'COR', NR'CON(R')2, NR'CO2R', COR', CO2R', OCOR', CON(R')2, OCON(R')2, SOR', SO2R', SO2N(R')2, NR'SO2R', NR'SO2N(R')2, COCOR', or COCH2COR'; each occurrence of R is independently hydrogen or an optionally substituted Cι_6 aliphatic group; and each occurrence of R is independently hydrogen or an optionally substituted Cι-6 aliphatic group, a 3-8-membered saturated, partially unsaturated, or fully unsaturated monocyclic ring having 0-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or an 8-12 membered saturated, partially unsaturated, or fully unsaturated bicyclic ring system having 0-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or R and R , two occurrences of R, or two occurrences of R , are taken together with the atom(s) to which they are bound to form an optionally substituted 3-12 membered saturated, partially unsaturated, or fully unsaturated monocyclic or bicyclic ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
Z1 is N or CH;
Z7 is N or C-URY;
T and U are each independently a bond or a saturated or unsaturated Cι_6 alkylidene chain, wherein up to two methylene units of the chain are optionally and independently replaced by CO, CO2, COCO, CONR, OCONR, NRNR, NRNRCO, NRCO, NRCO2, NRCONR, SO, SO2, NRSO2, SO2NR, NRSO2NR, O, S, or NR;
Rx and Rγ are each independently halogen, CN, NO2, or R';
Z2, Z5 and Z6 are each independently N or CH, provided that no more than two of Z2, Z5, and Z6 are N;
Z3 is CR3;
Z4 is CR4; wherein one of R3 or R4 is Ru, and the other of R3 or R4 is RV1, wherein: Ru is (CH2)tCN, (CH2)tNO2, (CH2)tN(R)2, (CH2)tNRC(O)R, (CH2)tCON(R)2, (CH2)tCOOR, (CH2)tSO2N(R)2, (CH2)tNRSO2R, (CH2)tNRCON(R)2, (CH2)tNRSO2N(R)2, (CH2)tCOCOR, (CH2)tAr2, wherein t is 0, 1, or 2, and Ar2 is an optionally substituted 5-7 membered saturated, partially unsaturated, or fully unsaturated monocyclic ring having 0-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur; and RV1 is OR'.
[0045] In certain embodiments, for compounds of general formula I: a) when Z1 is N, and Z7 is CH; then R1 is not phenyl only substituted with two or three occurrences of OR'; b) when Z1 is N, Z7 is CH; and ring B is phenyl, then R1 is not phenyl substituted in the meta position with nitro, fluorine-substituted lower alkoxy, or - NRCOOR', NRCON(R')2, NRCSOR', or NRCSN(R')2. ; and c) when Z1 is N, Z7 is CH, and ring B is pyridyl, pyrazinyl, pyrimidinyl, or pyridazinyl substituted with one or more occurrences of halogen, cyano, carbamoyl, COOR, COR, SO2N(R)2, N(R)2, OR, or fluorine substituted lower alkyl, then R1 is not phenyl substituted in the meta position with halogen, cyano, carbamoyl, COOR, COR, SO2N(R)2, N(R)2, OR, or fluorine substituted lower alkyl.
[0046] In yet other embodiments, for the compounds of formula I described generally above, those compounds where TRX is halogen are excluded.
[0047] In still other embodiments, for the compounds of formula I described generally above, those compounds where TRX is cyano are excluded.
[0048] In certain other embodiments, for the compounds of formula I described generally above, those compounds where TRX is alkynyl are excluded.
[0049] 2. Compounds and Definitions:
[0050] Compounds of this invention include those described generally above, and are further illustrated by the classes, subclasses, and species disclosed herein. As used herein, the following definitions shall apply unless otherwise indicated. For purposes of this invention, the chemical elements are identified in accordance with the Periodic Table of the Elements, CAS version, Handbook of Chemistry and Physics, 75th Ed. Additionally, general principles of organic chemistry are described in "Organic Chemistry", Thomas Sorrell, University Science Books, Sausalito: 1999, and "March's Advanced Organic Chemistry", 5th Ed., Ed.: Smith, M.B. and March, J., John Wiley & Sons, New York: 2001, the entire contents of which are hereby incorporated by reference.
[0051] As described herein, compounds of the invention may optionally be substituted with one or more substituents, such as are illustrated generally above, or as exemplified by particular classes, subclasses, and species of the invention. It will be appreciated that the phrase "optionally substituted" is used interchangeably with the phrase "substituted or unsubstituted." In general, the term "substituted", whether preceded by the term "optionally" or not, refers to the replacement of hydrogen radicals in a given structure with the radical of a specified substituent. Unless otherwise indicated, an optionally substituted group may have a substituent at each substitutable position of the group, and when more than one position in any given structure may be substituted with more than one substituent selected from a specified group, the substituent may be either the same or different at every position. Combinations of substituents envisioned by this invention are preferably those that result in the formation of stable or chemically feasible compounds. The term "stable", as used herein, refers to compounds that are not substantially altered when subjected to conditions to allow for their production, detection, and preferably their recovery, purification, and use for one or more of the purposes disclosed herein. In some embodiments, a stable compound or chemically feasible compound is one that is not substantially altered when kept at a temperature of 40°C or less, in the absence of moisture or other chemically reactive conditions, for at least a week.
[0052] The term "aliphatic" or "aliphatic group", as used herein, means a straight-chain (i.e., unbranched) or branched, substituted or unsubstituted hydrocarbon chain that is completely saturated or that contains one or more units of unsaturation, or a monocyclic hydrocarbon or bicyclic hydrocarbon that is completely saturated or that contains one or more units of unsaturation, but which is not aromatic (also referred to herein as "carbocycle" "cycloaliphatic" or "cycloalkyl"), that has a single point of attachment to the rest of the molecule. Unless otherwise specified, aliphatic groups contain 1-20 aliphatic carbon atoms. In some embodiments, aliphatic groups contain 1-10 aliphatic carbon atoms. In other embodiments, aliphatic groups contain 1-8 aliphatic carbon atoms. In still other embodiments, aliphatic groups contain 1-6 aliphatic carbon atoms, and in yet other embodiments aliphatic groups contain 1-4 aliphatic carbon atoms. In some embodiments, "cycloaliphatic" (or "carbocycle" or "cycloalkyl") refers to a monocyclic C3-C8 hydrocarbon or bicyclic C8-C12 hydrocarbon that is completely saturated or that contains one or more units of unsaturation, but which is not aromatic, that has a single point of attachment to the rest of the molecule wherein any individual ring in said bicyclic ring system has 3-7 members. Suitable aliphatic groups include, but are not limited to, linear or branched, substituted or unsubstituted alkyl, alkenyl, alkynyl groups and hybrids thereof such as (cycloalkyl)alkyl, (cycloalkenyl)alkyl or (cycloalkyl)alkenyl.
[0053] The term "heteroaliphatic", as used herein, means aliphatic groups wherein one or two carbon atoms are independently replaced by one or more of oxygen, sulfur, nitrogen, phosphorus, or silicon. Heteroaliphatic groups may be substituted or unsubstituted, branched or unbranched, cyclic or acyclic, and include "heterocycle", "heterocyclyl", "heterocycloaliphatic", or "heterocyclic" groups.
[0054] The term "heterocycle", "heterocyclyl", "heterocycloaliphatic", or "heterocyclic" as used herein means non-aromatic, monocyclic, bicyclic, or tricyclic ring systems in which one or more ring members are an independently selected heteroatom. In some embodiments, the "heterocycle", "heterocyclyl", "heterocycloaliphatic", or "heterocyclic" group has three to fourteen ring members in which one or more ring members is a heteroatom independently selected from oxygen, sulfur, nitrogen, or phosphorus, and each ring in the system contains 3 to 7 ring members.
[0055] The term "heteroatom" means one or more of oxygen, sulfur, nitrogen, phosphorus, or silicon (including, any oxidized form of nitrogen, sulfur, phosphorus, or silicon; the quaternized form of any basic nitrogen or; a substitutable nitrogen of a heterocyclic ring, for example N (as in 3,4-dihydro-2H-pyrrolyl), NΗ (as in pyrrolidinyl) or
NR+ (as in N-substituted pyrrolidinyl)).
[0056] The term "unsaturated", as used herein, means that a moiety has one or more units of unsaturation.
[0057] The term "alkoxy", or "thioalkyl", as used herein, refers to an alkyl group, as previously defined, attached to the principal carbon chain through an oxygen ("alkoxy") or sulfur ("thioalkyl") atom.
[0058] The terms "haloalkyl", "haloalkenyl" and "haloalkoxy" means alkyl, alkenyl or alkoxy, as the case may be, substituted with one or more halogen atoms. The term "halogen" means F, Cl, Br, or I.
[0059] The term "aryl" used alone or as part of a larger moiety as in "aralkyl",
"aralkoxy", or "aryloxyalkyl", refers to monocyclic, bicyclic, and tricyclic ring systems having a total of five to fourteen ring members, wherein at least one ring in the system is aromatic and wherein each ring in the system contains 3 to 7 ring members. The term "aryl" may be used interchangeably with the term "aryl ring". The term "aryl" also refers to heteroaryl ring systems as defined hereinbelow.
[0060] The term "heteroaryl", used alone or as part of a larger moiety as in
"heteroaralkyl" or "heteroarylalkoxy", refers to monocyclic, bicyclic, and tricyclic ring systems having a total of five to fourteen ring members, wherein at least one ring in the system is aromatic, at least one ring in the system contains one or more heteroatoms, and wherein each ring in the system contains 3 to 7 ring members. The term "heteroaryl" may be used interchangeably with the term "heteroaryl ring" or the term "heteroaromatic".
[0061] An aryl (including aralkyl, aralkoxy, aryloxyalkyl and the like) or heteroaryl
(including heteroaralkyl and heteroarylalkoxy and the like) group may contain one or more substituents and thus may be "optionally substituted". Unless otherwise defined above and herein, suitable substituents on the unsaturated carbon atom of an aryl or heteroaryl group are generally selected from halogen; -R°; -OR°; -SR°; phenyl (Ph) optionally substituted with R°; -O(Ph) optionally substituted with R°; -(CH2)ι.2(Ph), optionally substituted with R°; - CH=CH(Ph), optionally substituted with R°; -NO2; -CN; -N(R°)2; -NR°C(O)R°; - NR°C(S)R°; -NR°C(O)N(R°)2; -NR°C(S)N(R°)2; -NR°CO2R°; -NR°NR°C(O)R°; -NR°NR°C(O)N(R°)2; -NR°NR°CO2R°; -C(O)C(O)R°; -C(O)CH2C(O)R°; -CO2R°; - C(O)R°; -C(S)R°; -C(O)N(R°)2; -C(S)N(R°)2; -OC(O)N(R°)2; -OC(O)R°; -C(O)N(OR°) R°; -C(NOR°) R°; -S(O)2R°; -S(O)3R°; -SO2N(R°)2; -S(O)R°; -NR°SO2N(R°)2; -NR°SO2R°; -N(OR°)R°; -C(=NH)-N(R°)2; -P(O)2R°; -PO(R°)2; -OPO(R°)2; -(CH2)0.2NHC(O)Ro; phenyl (Ph) optionally substituted with R°; -O(Ph) optionally substituted with R°; -(CH2)ι_2(Ph), optionally substituted with R°; or -CH=CH(Ph), optionally substituted with R°; wherein each independent occurrence of R° is selected from hydrogen, optionally substituted Cι_6 aliphatic, an unsubstituted 5-6 membered heteroaryl or heterocyclic ring, phenyl, -O(Ph), or -CH (Ph), or, notwithstanding the definition above, two independent occurrences of R°, on the same substituent or different substituents, taken together with the atom(s) to which each R° group is bound, to form an optionally substituted 3-12 membered saturated, partially unsaturated, or fully unsaturated monocyclic or bicyclic ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur.
[0062] . Optional substituents on the aliphatic group of R° are selected from NH2, NH(Cι.4aliphatic), N(C1- aliphatic)2, halogen,
Figure imgf000016_0001
NO2, CN, CO2H, CO2(C1_4aliphatic), O aloC aliphatic), or
Figure imgf000016_0002
wherein each of the foregoing C^aliphatic groups of R° is unsubstituted.
[0063] An aliphatic or heteroaliphatic group, or a non-aromatic heterocyclic ring may contain one or more substituents and thus may be "optionally substituted". Unless otherwise defined above and herein, suitable substituents on the saturated carbon of an aliphatic or heteroaliphatic group, or of a non-aromatic heterocyclic ring are selected from those listed above for the unsaturated carbon of an aryl or heteroaryl group and additionally include the following: =O, =S, =NNHR*, =NN(R*)2, =NNHC(O)R*, =NNHCO2(alkyl), =NNHSO (alkyl), or =NR , where each R is independently selected from hydrogen or an optionally substituted Cι-6 aliphatic group.
[0064] Unless otherwise defined above and herein, optional substituents on the nitrogen of a non-aromatic heterocyclic ring are generally selected from -R+, -N(R+) , -C(O)R+, -CO2R+, -C(O)C(O)R+, -C(O)CH2C(O)R+, -SO2R+, -SO2N(R+)2, -C(=S)N(R+1)2, -C(=NH)- N(R+)2, or -NR+SO2R+; wherein R+ is hydrogen, an optionally substituted Cι.6 aliphatic, optionally substituted phenyl, optionally substituted -O(Ph), optionally substituted -CH2(Ph), optionally substituted -(CH2)ι_2(Ph); optionally substituted -CH=CH(Ph); or an unsubstituted 5-6 membered heteroaryl or heterocyclic ring having one to four heteroatoms independently selected from oxygen, nitrogen, or sulfur, or, notwithstanding the definition above, two independent occurrences of R+, on the same substituent or different substituents, taken together with the atom(s) to which each R+ group is bound, form an optionally substituted 3- 12 membered saturated, partially unsaturated, or fully unsaturated monocyclic or bicyclic ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur. [0065] Optional substituents on the aliphatic group or the phenyl ring of R+ are selected from -NH2, -NH(Ci_4 aliphatic), -N(Cι_4 aliphatic) , halogen, _ aliphatic, -OH, -0(Cμ aliphatic), -NO2, -CN, -CO2H, -CO2(CM aliphatic), -O(halo CM aliphatic), or halo(Cι_4 aliphatic), wherein each of the foregoing C^aliphatic groups of R is unsubstituted. [0066] The term "alkylidene chain" refers to a straight or branched carbon chain that may be fully saturated or have one or more units of unsaturation and has two points of attachment to the rest of the molecule.
[0067] As detailed above, in some embodiments, two independent occurrences of R° (or R+, R, R' or any other variable similarly defined herein), are taken together with the atom(s) to which they are bound to form an optionally substituted 3-12 membered saturated, partially unsaturated, or fully unsaturated monocyclic or bicyclic ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur.
[0068] Exemplary rings that are formed when two independent occurrences of R° (or R+, R, R' or any other variable similarly defined herein), are taken together with the atom(s) to which each variable is bound include, but are not limited to the following: a) two independent occurrences of R° (or R+, R, R' or any other variable similarly defined herein) that are bound to the same atom and are taken together with that atom to form a ring, for example, N(R°)2, where both occurrences of R° are taken together with the nitrogen atom to form a piperidin-1-yl, piperazin-1-yl, or morpholin-4-yl group; and b) two independent occurrences of R° (or R+, R, R' or any other variable similarly defined herein) that are bound to different atoms and are taken together with both of those atoms to form a ring, for example
where a phenyl group is substituted with two occurrences of OR0
Figure imgf000017_0001
these two occurrences of R° are taken together with the oxygen atoms to which they are bound to form
a fused 6-membered oxygen containing ring:
Figure imgf000017_0002
It will be appreciated that a variety of other rings can be formed when two independent occurrences of R° (or R , R, R' or any other variable similarly defined herein) are taken together with the atom(s) to which each variable is bound and that the examples detailed above are not intended to be limiting. [0069] Unless otherwise stated, structures depicted herein are also meant to include all isomeric (e.g., enantiomeric, diastereomeric, and geometric (or conformational)) forms of the structure; for example, the R and S configurations for each asymmetric center, (Z) and (E) double bond isomers, and (Z) and (E) conformational isomers. Therefore, single stereochemical isomers as well as enantiomeric, diastereomeric, and geometric (or conformational) mixtures of the present compounds are within the scope of the invention. Unless otherwise stated, all tautomeric forms of the compounds of the invention are within the scope of the invention. Additionally, unless otherwise stated, structures depicted herein are also meant to include compounds that differ only in the presence of one or more isotopically enriched atoms. For example, compounds having the present structures except for the replacement of hydrogen by deuterium or tritium, or the replacement of a carbon by a 13C- or l C-enriched carbon are within the scope of this invention. Such compounds are useful, for example, as analytical tools or probes in biological assays.
[0070] 3. Description of Exemplary Compounds:
[0071] In certain exemplary embodiments, Z1 is N and amino pyrimidines of general formula II are provided:
Figure imgf000018_0001
II
wherein R , UR , TR , Z , Z , Z , Z , and Z are as defined generally above and in classes and subclasses herein.
[0072] In certain other exemplary embodiments, Z1 is CH and amino pyridines of general formula III are provided:
Figure imgf000019_0001
wherein R1, URY, TRX, Z2, Z3, Z4, Z5, and Z6 are as defined generally above and in classes and subclasses herein.
[0073] As described generally above, R1 is Q-Ar1. Certain exemplary substituents for R1 include optionally substituted group selected from phenyl, cyclohexyl, cyclopentyl, pyridyl, naphthyl, morpholino, piperazinyl, or piperidinyl. In other embodiments, R1 is an optionally substituted group selected from phenyl, cyclohexyl, or pyridyl. In still other embodiments, R1 is optionally substituted phenyl.
[0074] As described generally above, in certain embodiments, Ar1 is substituted with 0-5 occurrences of ZRZ. Exemplary ZRZ groups are selected from halogen, R', CN, NO2, - N(R)(R'), -OR', -SR', -S(O)2N(R)(R'), CO(CMalkyl), -NRSO2R', COO(CMalkyl). In other embodiments, q is 1 or 2 and ZRZ is F, Cl, Br, COO(CMalkyl), Cι_4alkyl, CN, NO2, -NH2, - OH,
Figure imgf000019_0002
-S(O)2NH2, or an optionally substituted benzyloxy, phenyloxy, or phenyl group. In yet other embodiments, q is 1, and ZRZ is in the meta or para position and ZRZ is F, Cl, Br, methyl, ethyl, benzyloxy, phenyl, phenyloxy, COO(C1. alkyl), -NH2, -OH, ^alkoxy, or -S(O)2NH2.
[0075] Exemplary TRX and URY groups of formula I, and classes and subclasses thereof as described herein, are each independently hydrogen, halogen, NO2, CN, OR, SR or N(R)2, or Cι_ aliphatic optionally substituted with oxo, OR, SR, N(R) , halogen, NO2 or CN. In other embodiments, TRX and URY are each independently hydrogen, Me, OH, OMe or N(R)2. In yet other embodiments, TR and UR are each hydrogen.
[0076] In certain other embodiments, ring B is selected from one of the rings i-viii depicted below.
Figure imgf000019_0003
i ii iii
Figure imgf000020_0001
IV V VI
Figure imgf000020_0002
Vll VU1
[0077] In still other embodiments, ring B is i, ii, v, vi, or vii. In yet other embodiments,
[0078] As described generally above, one of R3 or R4 is Ru, and the other of R3 or R is RV1, wherein Ru is (CH2)tCN, (CH2)tNO2, (CH2)tN(R)2, (CH2)tNRC(O)R, (CH2)tCON(R)2, (CH2)tCOOR, (CH2)tSO2N(R)2, (CH2)tNRSO2R, (CH2)tNRCON(R)2, (CH2)tNRSO2N(R)2, (CH2)tCOCOR, (CH^tAr2, wherein t is 0, 1, or 2, and Ar2 is an optionally substituted 5-7 membered saturated, partially unsaturated, or fully unsaturated monocyclic ring having 0-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur; and Rvl is OR'. In certein exemplary embodiments Ar2 is an optionally substituted tetrazole, triazole, oxazole, thiazole, thiadiazole, oxadiazole or pyridyl group. In certain exemplary embodiments, compounds have one of the structures depicted below:
Figure imgf000020_0003
II-A III-A
Figure imgf000021_0001
II-B III-B
Figure imgf000021_0002
II-C III-C
Figure imgf000021_0003
II-D III-D
Figure imgf000021_0004
Figure imgf000021_0005
II-F III-F
Figure imgf000022_0001
II-G III-G
Figure imgf000022_0002
II-H III-H
Figure imgf000022_0003
II I III-I
Figure imgf000022_0004
II-J III-J
Figure imgf000023_0001
II-K III-K
Figure imgf000023_0002
II-L III-L
Figure imgf000023_0003
II-M III-M
Figure imgf000023_0004
II-N III-N
Figure imgf000024_0001
II-O III-O
Figure imgf000024_0002
II-P III-P
Figure imgf000024_0003
II-Q III-Q
Figure imgf000024_0004
II-R III-R
Figure imgf000024_0005
II S III-S
Figure imgf000025_0001
II-W III-W
Figure imgf000025_0002
II-X III-X
[0079] It will be appreciated that for compounds described above, ring B is selected from one of structures i, ii, v, vi, or vii. In certain exemplary embodiments, ring B is selected from i.
[0080] Certain subclasses of the foregoing compounds are described in more detail below. It will be appreciated that, for each of the compounds generally described above
(formula I) and classes thereof, any combination of the subsets set forth below may be utilized to describe exemplary subclasses of the invention, hi particular, certain preferred subsets include, but are not limited to the following:
[0081] i) compounds where R1 is selected from an optionally substituted group selected from phenyl, cyclohexyl, cyclopentyl, pyridyl, morpholino, piperazinyl, or piperidinyl;
[0082] ii) compounds where R1 is an optionally substituted group selected from phenyl, cyclohexyl, or pyridyl;
[0083] iii) compounds where R1 is optionally substituted phenyl;
[0084] iv) compounds where Ar1 is substituted with up to five occurrences of ZRZ, and
ZRZ groups are selected from halogen, R', CN, NO2, -N(R)(R'), -OR', -SR', -S(O)2N(R)(R'),
CO(Cι.4alkyl), -NRSO2R', COO(C1.4alkyl).
[0085] v) compounds where q is 1 and ZRZ is F, Cl, Br, COO(Cι.4alkyl), Cι_4alkyl, CN,
NO2, -NH2, -OH,
Figure imgf000026_0001
-S(O)2NH , or an optionally substituted benzyloxy, phenyloxy, or phenyl group;
7 7
[0086] vi) compounds where q is 1, and ZR is in the meta or para position and ZR is F,
Cl, Br, benzyloxy, phenyl, phenyloxy, COO(CMalkyl), -NH2, -OH,
Figure imgf000026_0002
or -
S(O)2NH2;
[0087] vii) compounds where TRX and URY are selected from hydrogen, halogen, NO2,
CN, OR, SR or N(R)2, or Cι_4aliphatic optionally substituted with oxo, OR, SR, N(R)2, halogen, NO or CN;
[0088] viii) compounds where TR and URY groups are selected from hydrogen, Me,
OH, OMe or N(R)2;
[0089] ix) compounds where TRX and URY are each hydrogen;
[0090] x) compounds where Z2 and Z6 are each CH;
[0091] xi) compounds where t is 0;
[0092] xii) compounds where t is 1; and [0093] xiii) compounds where Ar2 is an optionally substituted tetrazole, triazole, oxazole, thiazole, thiadiazole, oxadiazole or pyridyl group
[0094] Certain other exemplary embodiments relate to those compounds where R1 is optionally substituted phenyl and ring B is phenyl and compounds have one of the structures depicted below:
Figure imgf000027_0001
H-B-(i) III-B-(i)
Figure imgf000027_0002
II-C-(i) III-C-(i)
Figure imgf000028_0001
II-D-(i) III-D-(i)
Figure imgf000028_0002
II-E-(i) III-E-(i)
Figure imgf000028_0003
II-F-(i) III-F-(i)
Figure imgf000028_0004
II-G-(i) III-G-(i)
Figure imgf000028_0005
II-H-(i) III-H-(i)
Figure imgf000029_0001
II-I-(i) III-I-(i)
Figure imgf000029_0002
II-J-(i) III-J-(i)
Figure imgf000029_0003
II-K-(i) III-K-(i)
Figure imgf000029_0004
II-M-(i) III-M-(i)
Figure imgf000030_0001
II-N-(i) III-N-(i)
Figure imgf000030_0002
II-O-(i) III-O-(i)
Figure imgf000030_0003
π-Q-(i) III-Q-(i)
Figure imgf000031_0001
II-R-(i) III-R-(i)
Figure imgf000031_0002
II-S-(i) III-S-(i)
Figure imgf000031_0003
II-U-(i) III-U-(i)
Figure imgf000031_0004
II-V-(i) III-V-(i)
Figure imgf000032_0001
[0095] Certain exemplary embodiments also relate to those compounds where ring A is a ppyyrriimmiiddiinnee;; UURRY,, aanndd TTRRS are each hydrogen, and the compounds have the general structures depicted generally below:
Figure imgf000032_0002
II-A-(i)-a II-B-(i)-a
Figure imgf000032_0003
II-C-(i)-a II-D-(i)-a
Figure imgf000033_0001
II-E-(i)-a II-F-(i)-a
Figure imgf000033_0002
Figure imgf000033_0003
II-I-(i)-a II-J-(i)-a
Figure imgf000033_0004
II-K-(i)-a II-L-(i)-a
Figure imgf000033_0005
II-M-(i)-a II-N-(i)-a
Figure imgf000034_0001
II-O-(i)-a II-P-(i)-a
Figure imgf000034_0002
H-Q-(i)-a II-R-(i)-a
Figure imgf000034_0003
II-S-(i)-a II-T-(i)-a
Figure imgf000034_0004
II-U-(i)-a II-V-(i)-a
Figure imgf000035_0001
II-W-(i)-a II-X-(i)-a
[0096] Certain exemplary subsets for each of the compounds described above include those compounds where: a. q is 0, 1, or 2, and ZRZ is halogen, R', CN, NO2, -N(R)(R'), -OR', -SR', - S(O)2N(R)(R'), CO(CMalkyl), -NRSO2R', COO(Cwalkyl); b. t is O; c. R is optionally substituted Cι_6alkyl or hydrogen; and d. R' is optionally substituted Cι_6alkyl or hydrogen.
[0097] Representative examples of compounds of formula II and III are set forth below in Tables 1 and 2 below.
[0098] Table 1. Examples of Compounds of Formula II:
Figure imgf000035_0002
II I 11-2 II-3
Figure imgf000035_0003
11-4 π- 5 II-
Figure imgf000036_0001
II- 7 II-8 II- 9
Figure imgf000036_0002
π-10 π-11 π-12
Figure imgf000036_0003
11-13 π-14 11-15
Figure imgf000036_0004
π-16 11-17 π-18
Figure imgf000036_0005
11-19 π-20 π-21
Figure imgf000037_0001
11-22 11-23 11-24
Figure imgf000037_0002
11-25 11-26 11-27
Figure imgf000037_0003
π-28 11-29 11-30
Figure imgf000037_0004
11-31 11-32 π-33
Figure imgf000037_0005
11-34 11-35
Figure imgf000038_0001
11-36 π-37 11-38
Figure imgf000038_0002
11-39 11-40 π-41
Figure imgf000038_0003
π-42 π-43 11-44
Figure imgf000038_0004
π-45 11-46 π-47
Figure imgf000038_0005
Figure imgf000039_0001
π-60 11-61 π-62
Figure imgf000039_0002
Figure imgf000040_0001
11-66 11-67 11-68
Figure imgf000040_0002
11-69 π-70 11-71
Figure imgf000040_0003
11-72 11-73 π-74
Figure imgf000040_0004
Figure imgf000040_0005
π-78 11-79 11-80
Figure imgf000041_0001
11-81 11-82 π-83
Figure imgf000041_0002
π-87 π-88 11-89
Figure imgf000041_0003
-90 π-9i π-92
Figure imgf000042_0001
11-93 11-94 11-95
Figure imgf000042_0002
11-96 π-97 π-98
Figure imgf000042_0003
π-99 II-100 II-101
Figure imgf000042_0004
π-102 π-103 11-104
Figure imgf000042_0005
Figure imgf000043_0001
11-108 11-109 II-110
Figure imgf000043_0002
11-111 π-112 π-113
Figure imgf000043_0003
Figure imgf000043_0004
11-117 π-118 π-119
Figure imgf000043_0005
π-120 π-121 11-122
Figure imgf000044_0001
11-123 11-124 11-125
Figure imgf000044_0002
11-126 11-127 11-128
Figure imgf000044_0003
11-129 π-130 π-131
Figure imgf000044_0004
11-132 11-133 11-134
Figure imgf000044_0005
-135 11-136 11-137
Figure imgf000045_0001
11-138 11-139 π-140
Figure imgf000045_0002
-141 -142 11-143
Figure imgf000045_0003
11-144 11-145 11-146
Figure imgf000045_0004
11-147 11-148 11-149
Figure imgf000045_0005
Figure imgf000046_0001
11-153 11-154 11-155
] Table 2. Examples of Compounds of Formula III:
Figure imgf000046_0002
III-l 111-2 111-3
Figure imgf000046_0003
111-4 111-5 ιπ-6
Figure imgf000046_0004
Figure imgf000046_0005
Figure imgf000047_0001
Figure imgf000047_0002
111-22 111-23 111-24
Figure imgf000048_0001
111-25 111-26 111-27
Figure imgf000048_0002
Figure imgf000048_0003
m-31 111-32 111-33 111-34
[00100] 4. General Synthetic Methodology:
[00101] The compounds of this invention may be prepared in general by methods known to those skilled in the art for analogous compounds, as illustrated by Schemes 1, 2 and 3 below, and the preparative examples that follow.
[00102] Schemes 1, 2 and 3 below depict generally the synthesis of certain exemplary compounds of the invention. Specifically, Scheme 1 depicts the synthesis of compounds where R3 is COOH or CON(R)2 and R4 is OH. Scheme 2 depicts compounds where R3 is
NO2 or N(R)2 and R4 is OR'. Scheme 3 depicts compounds where R3 is CON(R)2 and R4 is
OR'. It will be appreciated that additional compounds where R3 is defined generally and in subsets herein can be prepared according to the general methods described above and methods known in the art using the appropriate starting materials.
[00103] Scheme 1:
YNH2
Figure imgf000049_0001
J z 2 ! procedure j procedut G X OR
Figure imgf000049_0002
Figure imgf000049_0003
[00104] Scheme 2:
Procedure A, B
Figure imgf000049_0005
Figure imgf000049_0004
procedure κ
Figure imgf000049_0006
Figure imgf000049_0007
[00105] Scheme 3: Procedure A, B
Figure imgf000050_0002
Figure imgf000050_0001
Figure imgf000050_0003
[00106] 5. Uses, Formulation and Administration [00107] Pharmaceutically acceptable compositions
[00108] As discussed above, the present invention provides compounds that are inhibitors of protein kinases, and thus the present compounds are useful for the treatment of diseases, disorders, and conditions including, but not limited to a proliferative disorder, a cardiac disorder, a neurodegenerative disorder, psychotic disorders, an autoimmune disorder, a condition associated with organ transplant, an inflammatory disorder, an immunologically mediated disorder, a viral disease, or a bone disorder. In preferred embodiments, the compounds are useful for the treatment of allergy, asthma, diabetes, Alzheimer's disease, Huntington's disease, Parkinson's disease, AIDS-associated dementia, amyotrophic lateral sclerosis (AML, Lou Gehrig's disease), multiple sclerosis (MS), schizophrenia, cardiomyocyte hypertrophy, reperfusion/ischemia (e.g., stroke), baldness, cancer, hepatomegaly, cardiovascular disease including cardiomegaly, cystic fibrosis, viral disease, autoimmune diseases, atherosclerosis, restenosis, psoriasis, inflammation, hypertension, angina pectoris, cerebrovascular contraction, peripheral circulation disorder, premature birth, arteriosclerosis, vasospasm (cerebral vasospasm, coronary vasospasm), retinopathy, erectile dysfunction (ED), AIDS, osteoporosis, Crohn's Disease and colitis, neurite outgrowth, and Raynaud's Disease. In preferred embodiments, the disease, condition, or disorder is atherosclerosis, hypertension, erectile dysfunction (ED), reperfusion/ischemia (e.g., stroke), or vasospasm (cerebral vasospasm and coronary vasospasm).
[00109] Accordingly, in another aspect of the present invention, pharmaceutically acceptable compositions are provided, wherein these compositions comprise any of the compounds as described herein, and optionally comprise a pharmaceutically acceptable carrier, adjuvant or vehicle. In certain embodiments, these compositions optionally further comprise one or more additional therapeutic agents.
[00110] It will also be appreciated that certain of the compounds of present invention can exist in free form for treatment, or where appropriate, as a pharmaceutically acceptable derivative thereof. According to the present invention, a pharmaceutically acceptable derivative includes, but is not limited to, pharmaceutically acceptable prodrugs, salts, esters, salts of such esters, or any other adduct or derivative which upon administration to a patient in need is capable of providing, directly or indirectly, a compound as otherwise described herein, or a metabolite or residue thereof.
[00111] As used herein, the term "pharmaceutically acceptable salt" refers to those salts which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of humans and lower animals without undue toxicity, irritation, allergic response and the like, and are commensurate with a reasonable benefit/risk ratio. A "pharmaceutically acceptable salt" means any non-toxic salt or salt of an ester of a compound of this invention that, upon administration to a recipient, is capable of providing, either directly or indirectly, a compound of this invention or an inhibitorily active metabolite or residue thereof. As used herein, the term "inhibitorily active metabolite or residue thereof means that a metabolite or residue thereof is also an inhibitor of a JAK-3, JNK-3, CDK-2, SYK, or GSK-3 kinase. [00112] Pharmaceutically acceptable salts are well known in the art. For example, S. M. Berge et al, describe pharmaceutically acceptable salts in detail in J. Pharmaceutical Sciences, 1977, 66, 1-19, incorporated herein by reference. Pharmaceutically acceptable salts of the compounds of this invention include those derived from suitable inorganic and organic acids and bases. Examples of pharmaceutically acceptable, nontoxic acid addition salts are salts of an amino group formed with inorganic acids such as hydrochloric acid, hydrobromic acid, phosphoric acid, sulfuric acid and perchloric acid or with organic acids such as acetic acid, oxalic acid, maleic acid, tartaric acid, citric acid, succinic acid or malonic acid or by using other methods used in the art such as ion exchange. Other pharmaceutically acceptable salts include adipate, alginate, ascorbate, aspartate, benzenesulfonate, benzoate, bisulfate, borate, butyrate, camphorate, camphorsulfonate, citrate, cyclopentanepropionate, digluconate, dodecylsulfate, ethanesulfonate, formate, fumarate, glucoheptonate, glycerophosphate, gluconate, hemisulfate, heptanoate, hexanoate, hydroiodide, 2-hydroxy-ethanesulfonate, lactobionate, lactate, laurate, lauryl sulfate, malate, maleate, malonate, methanesulfonate, 2- naphthalenesulfonate, nicotinate, nitrate, oleate, oxalate, palmitate, pamoate, pectinate, persulfate, 3-phenylpropionate, phosphate, picrate, pivalate, propionate, stearate, succinate, sulfate, tartrate, thiocyanate, p-toluenesulfonate, undecanoate, valerate salts, and the like. Salts derived from appropriate bases include alkali metal, alkaline earth metal, ammonium and N+(Cι. alkyl) salts. This invention also envisions the quaternization of any basic nitrogen-containing groups of the compounds disclosed herein. Water or oil-soluble or dispersable products may be obtained by such quaternization. Representative alkali or alkaline earth metal salts include sodium, lithium, potassium, calcium, magnesium, and the like. Further pharmaceutically acceptable salts include, when appropriate, nontoxic ammonium, quaternary ammonium, and amine cations formed using counterions such as halide, hydroxide, carboxylate, sulfate, phosphate, nitrate, loweralkyl sulfonate and aryl sulfonate.
[00113] As described above, the pharmaceutically acceptable compositions of the present invention additionally comprise a pharmaceutically acceptable carrier, adjuvant, or vehicle, which, as used herein, includes any and all solvents, diluents, or other liquid vehicle, dispersion or suspension aids, surface active agents, isotonic agents, thickening or emulsifying agents, preservatives, solid binders, lubricants and the like, as suited to the particular dosage form desired. Remington's Pharmaceutical Sciences, Sixteenth Edition, E. W. Martin (Mack Publishing Co., Easton, Pa., 1980) discloses various carriers used in formulating pharmaceutically acceptable compositions and known techniques for the preparation thereof. Except insofar as any conventional carrier medium is incompatible with the compounds of the invention, such as by producing any undesirable biological effect or otherwise interacting in a deleterious manner with any other component(s) of the pharmaceutically acceptable composition, its use is contemplated to be within the scope of this invention. Some examples of materials which can serve as pharmaceutically acceptable carriers include, but are not limited to, ion exchangers, alumina, aluminum stearate, lecithin, serum proteins, such as human serum albumin, buffer substances such as phosphates, glycine, sorbic acid, or potassium sorbate, partial glyceride mixtures of saturated vegetable fatty acids, water, salts or electrolytes, such as protamine sulfate, disodium hydrogen phosphate, potassium hydrogen phosphate, sodium chloride, zinc salts, colloidal silica, magnesium trisilicate, polyvinyl pyrrolidone, polyacrylates, waxes, polyethylene-polyoxypropylene- block polymers, wool fat, sugars such as lactose, glucose and sucrose; starches such as corn starch and potato starch; cellulose and its derivatives such as sodium carboxymethyl cellulose, ethyl cellulose and cellulose acetate; powdered tragacanth; malt; gelatin; talc; excipients such as cocoa butter and suppository waxes; oils such as peanut oil, cottonseed oil; safflower oil; sesame oil; olive oil; corn oil and soybean oil; glycols; such a propylene glycol or polyethylene glycol; esters such as ethyl oleate and ethyl laurate; agar; buffering agents such as magnesium hydroxide and aluminum hydroxide; alginic acid; pyrogen-free water; isotonic saline; Ringer's solution; ethyl alcohol, and phosphate buffer solutions, as well as other non-toxic compatible lubricants such as sodium lauryl sulfate and magnesium stearate, as well as coloring agents, releasing agents, coating agents, sweetening, flavoring and perfuming agents, preservatives and antioxidants can also be present in the composition, according to the judgment of the formulator.
[00114] Uses of Compounds and Pharmaceutically acceptable compositions [00115] In yet another aspect, a method for the treatment or lessening the severity of a proliferative disorder, a cardiac disorder, a neurodegenerative disorder, a psychotic disorder, an autoimmune disorder, a condition associated with organ transplant, an inflammatory disorder, an immunologically mediated disorder, a viral disease, or a bone disorder is provided comprising administering an effective amount of a compound, or a pharmaceutically acceptable composition comprising a compound to a subject in need thereof. In certain embodiments of the present invention an "effective amount" of the compound or pharmaceutically acceptable composition is that amount effective for treating or lessening the severity of a proliferative disorder, a cardiac disorder, a neurodegenerative disorder, a psychotic disorder, an autoimmune disorder, a condition associated with organ transplant, an inflammatory disorder, an immunologically mediated disorder, a viral disease, or a bone disorder. The compounds and compositions, according to the method of the present invention, may be administered using any amount and any route of administration effective for treating or lessening the severity of a proliferative disorder, a cardiac disorder, a neurodegenerative disorder, an autoimmune disorder, a condition associated with organ transplant, an inflammatory disorder, an immunologically mediated disorder, a viral disease, or a bone disorder. The exact amount required will vary from subject to subject, depending on the species, age, and general condition of the subject, the severity of the infection, the particular agent, its mode of administration, and the like. The compounds of the invention are preferably formulated in dosage unit form for ease of administration and uniformity of dosage. The expression "dosage unit form" as used herein refers to a physically discrete unit of agent appropriate for the patient to be treated. It will be understood, however, that the total daily usage of the compounds and compositions of the present invention will be decided by the attending physician within the scope of sound medical judgment. The specific effective dose level for any particular patient or organism will depend upon a variety of factors including the disorder being treated and the severity of the disorder; the activity of the specific compound employed; the specific composition employed; the age, body weight, general health, sex and diet of the patient; the time of administration, route of administration, and rate of excretion of the specific compound employed; the duration of the treatment; drugs used in combination or coincidental with the specific compound employed, and like factors well known in the medical arts. The term "patient", as used herein, means an animal, preferably a mammal, and most preferably a human.
[00116] The pharmaceutically acceptable compositions of this invention can be administered to humans and other animals orally, rectally, parenterally, intracisteπially, intravaginally, intraperitoneally, topically (as by powders, ointments, or drops), bucally, as an oral or nasal spray, or the like, depending on the severity of the infection being treated. In certain embodiments, the compounds of the invention may be administered orally or parenterally at dosage levels of about 0.01 mg/kg to about 50 mg/kg and preferably from about 1 mg/kg to about 25 mg/kg, of subject body weight per day, one or more times a day, to obtain the desired therapeutic effect.
[00117] Liquid dosage forms for oral administration include, but are not limited to, pharmaceutically acceptable emulsions, microemulsions, solutions, suspensions, syrups and elixirs. In addition to the active compounds, the liquid dosage forms may contain inert diluents commonly used in the art such as, for example, water or other solvents, solubilizing agents and emulsifiers such as ethyl alcohol, isopropyl alcohol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylene glycol, dimethylformamide, oils (in particular, cottonseed, groundnut, corn, germ, olive, castor, and sesame oils), glycerol, tetrahydrofurfuryl alcohol, polyethylene glycols and fatty acid esters of sorbitan, and mixtures thereof. Besides inert diluents, the oral compositions can also include adjuvants such as wetting agents, emulsifying and suspending agents, sweetening, flavoring, and perfuming agents.
[00118] Injectable preparations, for example, sterile injectable aqueous or oleaginous suspensions may be formulated according to the known art using suitable dispersing or wetting agents and suspending agents. The sterile injectable preparation may also be a sterile injectable solution, suspension or emulsion in a nontoxic parenterally acceptable diluent or solvent, for example, as a solution in 1,3-butanediol. Among the acceptable vehicles and solvents that may be employed are water, Ringer's solution, U.S. P. and isotonic sodium chloride solution. In addition, sterile, fixed oils are conventionally employed as a solvent or suspending medium. For this purpose any bland fixed oil can be employed including synthetic mono- or diglycerides. In addition, fatty acids such as oleic acid are used in the preparation of injectables.
[00119] The injectable formulations can be sterilized, for example, by filtration through a bacterial-retaining filter, or by incorporating sterilizing agents in the form of sterile solid compositions which can be dissolved or dispersed in sterile water or other sterile injectable medium prior to use.
[00120] In order to prolong the effect of a compound of the present invention, it is often desirable to slow the absorption of the compound from subcutaneous or intramuscular injection. This may be accomplished by the use of a liquid suspension of crystalline or amorphous material with poor water solubility. The rate of absorption of the compound then depends upon its rate of dissolution that, in turn, may depend upon crystal size and crystalline form. Alternatively, delayed absorption of a parenterally administered compound form is accomplished by dissolving or suspending the compound in an oil vehicle. Injectable depot forms are made by forming microencapsule matrices of the compound in biodegradable polymers such as polylactide-polyglycolide. Depending upon the ratio of compound to polymer and the nature of the particular polymer employed, the rate of compound release can be controlled. Examples of other biodegradable polymers include poly(orthoesters) and poly(anhydrides). Depot injectable formulations are also prepared by entrapping the compound in liposomes or microemulsions that are compatible with body tissues. [00121] Compositions for rectal or vaginal administration are preferably suppositories which can be prepared by mixing the compounds of this invention with suitable non-irritating excipients or carriers such as cocoa butter, polyethylene glycol or a suppository wax which are solid at ambient temperature but liquid at body temperature and therefore melt in the rectum or vaginal cavity and release the active compound.
[00122] Solid dosage forms for oral administration include capsules, tablets, pills, powders, and granules. In such solid dosage forms, the active compound is mixed with at least one inert, pharmaceutically acceptable excipient or carrier such as sodium citrate or dicalcium phosphate and/or a) fillers or extenders such as starches, lactose, sucrose, glucose, mannitol, and silicic acid, b) binders such as, for example, carboxymethylcellulose, alginates, gelatin, polyvinylpyrrolidinone, sucrose, and acacia, c) humectants such as glycerol, d) disintegrating agents such as agar—agar, calcium carbonate, potato or tapioca starch, alginic acid, certain silicates, and sodium carbonate, e) solution retarding agents such as paraffin, f) absorption accelerators such as quaternary ammonium compounds, g) wetting agents such as, for example, cetyl alcohol and glycerol monostearate, h) absorbents such as kaolin and bentonite clay, and i) lubricants such as talc, calcium stearate, magnesium stearate, solid polyethylene glycols, sodium lauryl sulfate, and mixtures thereof. In the case of capsules, tablets and pills, the dosage form may also comprise buffering agents.
[00123] Solid compositions of a similar type may also be employed as fillers in soft and hard-filled gelatin capsules using such excipients as lactose or milk sugar as well as high molecular weight polyethylene glycols and the like. The solid dosage forms of tablets, dragees, capsules, pills, and granules can be prepared with coatings and shells such as enteric coatings and other coatings well known in the pharmaceutical formulating art. They may optionally contain opacifying agents and can also be of a composition that they release the active ingredient(s) only, or preferentially, in a certain part of the intestinal tract, optionally, in a delayed manner. Examples of embedding compositions that can be used include polymeric substances and waxes. Solid compositions of a similar type may also be employed as fillers in soft and hard-filled gelatin capsules using such excipients as lactose or milk sugar as well as high molecular weight polethylene glycols and the like.
[00124] The active compounds can also be in micro-encapsulated form with one or more excipients as noted above. The solid dosage forms of tablets, dragees, capsules, pills, and granules can be prepared with coatings and shells such as enteric coatings, release controlling coatings and other coatings well known in the pharmaceutical formulating art. In such solid dosage forms the active compound may be admixed with at least one inert diluent such as sucrose, lactose or starch. Such dosage forms may also comprise, as is normal practice, additional substances other than inert diluents, e.g., tableting lubricants and other tableting aids such a magnesium stearate and microcrystalline cellulose. In the case of capsules, tablets and pills, the dosage forms may also comprise buffering agents. They may optionally contain opacifying agents and can also be of a composition that they release the active ingredient(s) only, or preferentially, in a certain part of the intestinal tract, optionally, in a delayed manner. Examples of embedding compositions that can be used include polymeric substances and waxes.
[00125] Dosage forms for topical or transdermal administration of a compound of this invention include ointments, pastes, creams, lotions, gels, powders, solutions, sprays, inhalants or patches. The active component is admixed under sterile conditions with a pharmaceutically acceptable carrier and any needed preservatives or buffers as may be required. Ophthalmic formulation, ear drops, and eye drops are also contemplated as being within the scope of this invention. Additionally, the present invention contemplates the use of transdermal patches, which have the added advantage of providing controlled delivery of a compound to the body. Such dosage forms can be made by dissolving or dispensing the compound in the proper medium. Absorption enhancers can also be used to increase the flux of the compound across the skin. The rate can be controlled by either providing a rate controlling membrane or by dispersing the compound in a polymer matrix or gel. [00126] As described generally above, the compounds of the invention are useful as inhibitors of protein kinases. In one embodiment, the compounds and compositions of the invention are inhibitors of one or more of JAK-3, JNK-3, CDK-2, SYK, or GSK-3, and thus, without wishing to be bound by any particular theory, the compounds and compositions are particularly useful for treating or lessening the severity of a disease, condition, or disorder where activation of one or more of JAK-3, JNK-3, CDK-2, SYK, or GSK-3 is implicated in the disease, condition, or disorder. When activation of JAK-3, JNK-3, CDK-2, SYK, or GSK-3 is implicated in a particular disease, condition, or disorder, the disease, condition, or disorder may also be referred to as "JAK-3, JNK-3, CDK-2, SYK, or GSK-3 -mediated disease" or disease symptom. Accordingly, in another aspect, the present invention provides a method for treating or lessening the severity of a disease, condition, or disorder where activation or one or more of JAK-3, JNK-3, CDK-2, SYK, or GSK-3 is implicated in the disease state.
[00127] The activity of a compound utilized in this invention as an inhibitor of JAK-3, JNK-3, CDK-2, SYK, or GSK-3, may be assayed in vitro, in vivo or in a cell line. In vitro assays include assays that determine inhibition of either the phosphorylation activity or ATPase activity of activated JAK-3, JNK-3, CDK-2, SYK, or GSK-3. Alternate in vitro assays quantitate the ability of the inhibitor to bind to JAK-3, JNK-3, CDK-2, SYK, or GSK- 3. Inhibitor binding may be measured by radiolabelling the inhibitor prior to binding, isolating the inhibitor/ JAK-3, inhibitor/JNK-3, inhibitor/CDK-2, inhibitor/GSK-3, or inhibitor/SYK complex and determining the amount of radiolabel bound. Alternatively, inhibitor binding may be determined by running a competition experiment where new inhibitors are incubated with JAK-3, JNK-3, CDK-2, SYK, or GSK-3 bound to known radioligands.
[00128] The term "measurably inhibit", as used herein means a measurable change in JAK-3, JNK-3, CDK-2, SYK, or GSK-3 activity between a sample comprising said composition and a JAK-3, JNK-3, CDK-2, SYK, or GSK-3 kinase and an equivalent sample comprising JAK-3, JNK-3, CDK-2, SYK, or GSK-3 kinase in the absence of said composition. [00129] The term "JAK-mediated disease", as used herein means any disease or other deleterious condition in which a JAK family kinase is known to play a role. Such conditions include, without limitation, immune responses such as allergic or type I hypersensitivity reactions, asthma, autoimmune diseases such as transplant rejection, graft versus host disease, rheumatoid arthritis, amyotrophic lateral sclerosis, and multiple sclerosis, neurodegenerative disorders such as Familial amyotrophic lateral sclerosis (FALS), as well as in solid and hematologic malignancies such as leukemias and lymphomas.
[00130] According to another embodiment, the invention provides a method for treating or lessening the severity of a CDK2-mediated disease or condition in a patient comprising the step of administering to said patient a composition according to the present invention. [00131] The term "CDK2-mediated disease", as used herein means any disease or other deleterious condition in which CDK2 is known to play a role. Accordingly, these compounds are useful for treating diseases or conditions that are known to be affected by the activity of CDK2 kinase. Such diseases or conditions include cancer, Alzheimer's disease, restenosis, angiogenesis, glomerulonephritis, cytomegalovirus, HIN, herpes, psoriasis, atherosclerosis, alopecia, and autoimmune diseases such as rheumatoid arthritis, viral infections, neurodegenerative disorders, disorders associated with thymocyte apoptosis, or proliferative disorders resulting from the deregulation of the cell cycle, especially of the progression from Gi to S phase.
[00132] According to another embodiment, the invention provides a method for treating or lessening the severity of a CDK2 -mediated disease or condition in a patient comprising the step of administering to said patient a composition according to the present invention. [00133] The term "JΝK-mediated condition", as used herein means any disease or other deleterious condition in which JΝK is known to play a role. Such conditions include, without limitation, inflammatory diseases, autoimmune diseases, destructive bone disorders, proliferative disorders, cancer, infectious diseases, neurodegenerative diseases, allergies, reperfusion/ischemia in stroke, heart attacks, angiogenic disorders, organ hypoxia, vascular hyperplasia, cardiac hypertrophy, thrombin-induced platelet aggregation, and conditions associated with prostaglandin endoperoxidase synthase-2.
[00134] "JΝK-mediated conditions" also include ischemia/reperfusion in stroke, heart attacks, myocardial ischemia, organ hypoxia, vascular hyperplasia, cardiac hypertrophy, hepatic ischemia, liver disease, congestive heart failure, pathologic immune responses such as that caused by T cell activation and thrombin-induced platelet aggregation. [00135] In addition, JNK inhibitors of the instant invention may be capable of inhibiting the expression of inducible pro-inflammatory proteins. Therefore, other "JNK-mediated conditions" which may be treated by the compounds of this invention include edema, analgesia, fever and pain, such as neuromuscular pain, headache, cancer pain, dental pain and arthritis pain.
[00136] According to another embodiment, the invention provides a method for treating or lessening the severity of a GSK3 -mediated disease or condition in a patient comprising the step of administering to said patient a composition according to the present invention. [00137] The term "GSK3 -mediated disease" or "GSK3 -mediated condition", as used herein, means any disease or other deleterious condition in which GSK3 protein kinase is known to play a role. Such conditions include, without limitation, diabetes, neurodegenerative disorders, Alzheimer's disease, Huntington's, Parkinson's, AIDS associated dementia, amyotrophic lateral sclerosis (AML), multiple sclerosis (MS), schizophrenia, stroke, cardiomycete hypertrophy, and baldness.
[00138] According to another embodiment, the invention provides a method for treating or lessening the severity of a Syk-mediated disease or condition in a patient comprising the step of administering to said patient a composition according to the present invention. [00139] The term "Syk-mediated disease" or "Syk-mediated condition", as used herein, means any disease or other deleterious condition in which Syk protein kinase is known to play a role. Such conditions include, without limitation, allergic disorders, especially asthma. [00140] It will also be appreciated that the compounds and pharmaceutically acceptable compositions of the present invention can be employed in combination therapies, that is, the compounds and pharmaceutically acceptable compositions can be administered concurrently with, prior to, or subsequent to, one or more other desired therapeutics or medical procedures. The particular combination of therapies (therapeutics or procedures) to employ in a combination regimen will take into account compatibility of the desired therapeutics and/or procedures and the desired therapeutic effect to be achieved. It will also be appreciated that the therapies employed may achieve a desired effect for the same disorder (for example, an inventive compound may be administered concurrently with another agent used to treat the same disorder), or they may achieve different effects (e.g., control of any adverse effects). As used herein, additional therapeutic agents that are normally administered to treat or prevent a particular disease, or condition, are known as "appropriate for the disease, or condition, being treated". [00141] For example, chemotherapeutic agents or other anti-proliferative agents may be combined with the compounds of this invention to treat proliferative diseases and cancer. Examples of known chemotherapeutic agents include, but are not limited to, For example, other therapies or anticancer agents that may be used in combination with the inventive anticancer agents of the present invention include surgery, radiotherapy (in but a few examples, gamma.-radiation, neutron beam radiotherapy, electron beam radiotherapy, proton therapy, brachytherapy, and systemic radioactive isotopes, to name a few), endocrine therapy, biologic response modifiers (interferons, interleukins, and tumor necrosis factor (TNF) to name a few), hyperthermia and cryotherapy, agents to attenuate any adverse effects (e.g., antiemetics), and other approved chemotherapeutic drugs, including, but not limited to, alkylating drugs (mechlorethamine, chlorambucil, Cyclophosphamide, Melphalan, Ifosfamide), antimetabolites (Methotrexate), purine antagonists and pyrimidine antagonists (6-Mercaptopurine, 5-Fluorouracil, Cytarabile, Gemcitabine), spindle poisons (Ninblastine, Nincristine, Ninorelbine, Paclitaxel), podophyllotoxins (Etoposide, Irinotecan, Topotecan), antibiotics (Doxorubicin, Bleomycin, Mitomycin), nitrosoureas (Carmustine, Lomustine), inorganic ions (Cisplatin, Carboplatin), enzymes (Asparaginase), and hormones (Tamoxifen, Leuprolide, Flutamide, and Megestrol), Gleevec™, adriamycin, dexamethasone, and cyclophosphamide. For a more comprehensive discussion of updated cancer therapies see, http://www.nci.nih.gov/, a list of the FDA approved oncology drugs at http://www.fda.gov/cder/cancer/druglistframe.htm, and The Merck Manual, Seventeenth Ed. 1999, the entire contents of which are hereby incorporated by reference. [00142] Other examples of agents the inhibitors of this invention may also be combined with include, without limitation: treatments for Alzheimer's Disease such as Aricept and Excelon®; treatments for Parkinson's Disease such as L-DOPA/carbidopa, entacapone, ropinrole, pramipexole, bromocriptine, pergolide, trihexephendyl, and amantadine; agents for treating Multiple Sclerosis (MS) such as beta interferon (e.g., Avonex® and Rebif®), Copaxone®, and mitoxantrone; treatments for asthma such as albuterol and Singulair®; agents for treating schizophrenia such as zyprexa, risperdal, seroquel, and haloperidol; anti- inflammatory agents such as corticosteroids, TΝF blockers, IL-1 RA, azathioprine, cyclophosphamide, and sulfasalazine; immunomodulatory and immunosuppressive agents such as cyclosporin, tacrolimus, rapamycin, mycophenolate mofetil, interferons, corticosteroids, cyclophosphamide, azathioprine, and sulfasalazine; neurotrophic factors such as acetylcholinesterase inhibitors, MAO inhibitors, interferons, anti-convulsants, ion channel blockers, riluzole, and anti-Parkinsonian agents; agents for treating cardiovascular disease such as beta-blockers, ACE inhibitors, diuretics, nitrates, calcium channel blockers, and statins; agents for treating liver disease such as corticosteroids, cholestyramine, interferons, and anti-viral agents; agents for treating blood disorders such as corticosteroids, anti- leukemic agents, and growth factors; and agents for treating immunodeficiency disorders such as gamma globulin.
[00143] The amount of additional therapeutic agent present in the compositions of this invention will be no more than the amount that would normally be administered in a composition comprising that therapeutic agent as the only active agent. Preferably the amount of additional therapeutic agent in the presently disclosed compositions will range from about 50% to 100% of the amount normally present in a composition comprising that agent as the only therapeutically active agent.
[00144] The compounds of this invention or pharmaceutically acceptable compositions thereof may also be incorporated into compositions for coating implantable medical devices, such as prostheses, artificial valves, vascular grafts, stents and catheters. Accordingly, the present invention, in another aspect, includes a composition for coating an implantable device comprising a compound of the present invention as described generally above, and in classes and subclasses herein, and a carrier suitable for coating said implantable device. In still another aspect, the present invention includes an implantable device coated with a composition comprising a compound of the present invention as described generally above, and in classes and subclasses herein, and a carrier suitable for coating said implantable device.
[00145] Vascular stents, for example, have been used to overcome restenosis (re- narrowing of the vessel wall after injury). However, patients using stents or other implantable devices risk clot formation or platelet activation. These unwanted effects may be prevented or mitigated by pre-coating the device with a pharmaceutically acceptable composition comprising a kinase inhibitor. Suitable coatings and the general preparation of coated implantable devices are described in US Patents 6,099,562; 5,886,026; and 5,304,121. The coatings are typically biocompatible polymeric materials such as a hydrogel polymer, polymethyldisiloxane, polycaprolactone, polyethylene glycol, polylactic acid, ethylene vinyl acetate, and mixtures thereof. The coatings may optionally be further covered by a suitable topcoat of fluorosilicone, polysaccarides, polyethylene glycol, phospholipids or combinations thereof to impart controlled release characteristics in the composition.
[00146] Another aspect of the invention relates to inhibiting JAK-3, JNK-3, CDK-2, SYK, or GSK-3 activity in a biological sample or a patient, which method comprises administering to the patient, or contacting said biological sample with a compound of formula I or a composition comprising said compound. The term "biological sample", as used herein, includes, without limitation, cell cultures or extracts thereof; biopsied material obtained from a mammal or extracts thereof; and blood, saliva, urine, feces, semen, tears, or other body fluids or extracts thereof.
[00147] Inhibition of JAK-3, JNK-3, CDK-2, SYK, or GSK-3 kinase activity in a biological sample is useful for a variety of purposes that are known to one of skill in the art. Examples of such purposes include, but are not limited to, blood transfusion, organ- transplantation, biological specimen storage, and biological assays.
[00148] In order that the invention described herein may be more fully understood, the following examples are set forth. It should be understood that these examples are for illustrative purposes only and are not to be construed as limiting this invention in any manner.
EXAMPLES
[00149] Scheme I above depicts the synthesis of several exemplary compounds. The examples below describe general procedures for the preparation of compounds herein (as shown in Schemes 1, 2 and 3 above) and Table 3 depicts characterization for exemplary compounds of the invention.
[00150] Preparation of guanidines.
[00151] Procedure A: general procedure for the synthesis of guanidines
[00152] The substituted aniline (20mmol, 2 eq.) and cyanamide (lOmmol, leq.) were taken up in Toluene (5ml), and Triflic acid (1ml). The reaction was sealed and heated to
85C, overnight, with magnetic stirring. The reaction was quenched with water (10ml). The phases were separated and the aqueous was made basic with 2N sodium hydroxide (10ml).
The basic aqueous phase was washed with toluene and then extracted with methylene chloride (3X) to give desired guanidine upon concentration.
[00153] Procedure B: general procedure for the synthesis of guanidines
[00154] In a tube was placed cyanamide (lOmmol, 1 eq.) and substituted aniline (1 lmmol,
1.1 eq). To this was added 10ml of dioxane (alternatively ethylene glycol dimethyl ether,
DME, can be used), and the mixture was warmed to achieve dissolution. To the homogeneous solution was added 4N hydrochloric acid in dioxane (3ml, 12mmol, 1.2eq.).
The tube was sealed and heated to 60C overnight with magnetic stirring. The reaction was concentrated to dryness, basified with 2N NaOH, and extracted with methylene chloride
(2X). The organics were concentraded to give desired guanidine.
[00155] Procedure B (modified): general procedure for the synthesis of guanidines
[00156] The substituted aniline (20mmol) and cyanamide (20mmol) were dissolved in dioxane (25ml) with warming. To this was added 4N hydrochloric acid in dioxane (5ml,
20mmol) dropwise via syringe. The reaction was heated to reflux for three days, concentrated to dryness and dissolved in ethanol. To this was added 2N sodium hydroxide
(10ml, 20mmol) resulting in a voluminous precipitate. The solid was filtered and washed with ether/ethanol, and then dried in-vacuo to give the desired guanidine with 1 equivalent of sodium chloride.
[00157] Procedure D: procedure for the synthesis of N-methylated benzoxazin eneaminones
[00158] The compound 6-acetyl-2H-l,4-benzoxazin-3(4H)-one (lOmmol) was taken up in excess N,N-dimethylformamide dimethyl acetal and heated to 80C, overnight. The reaction was concentrated to dryness and used without purification.
Procedure E: general procedure for the synthesis of N-alkylated benzoxazin acetophenones
[00159] The compound 6-acetyl-2H-l,4-benzoxazin-3(4H)-one (lOmmol) and alkylating agent (5.4mmol, 1.1 eq.) were taken up in dimethylformamide (10ml) with powdered potassium carbonate (36mmol, xs). The reaction was heated to approximately HOC for 1.5 to 24hours. The reaction is quenched with water and extracted with ether (2X). The organics were washed with brine, dried over sodium sulfate, filtered, and concentrated to give crude.
The crude was purified via flash chromatography on silica gel, and eluted with ether or ethyl acetate).
[00160] Procedure F: general procedure for the synthesis of eneaminones
[00161] The appropriate acetophenone was taken up in N,N-Dimethylformamide dimethyl acetal neat (alternatively toluene may be used as co-solvent), and heated to 95C for 1 to 3 days. Alternatively, toluene may be added to aid dissolution. The reaction was then concentrated to an oil. The product occasionally crystallized from ethyl acetate, or from ethyl acetate/hexane. Otherwise, it was purified via column chromatography on silica gel, eluted with ethyl acetate/hexane to pure ethyl acetate.
[00162] Procedure G: general procedure for the synthesis of eneaminones
[00163] The appropriate acetophenone (20mmol) was dissolved in 100 ml of toluene
(alternatively N,N-dimethylformamide or tetrahydrofuran may be used as solvent) and treated with tert-Butoxybis (dimethylamino) methane (Bredereck's reagent, 35mmol, 1.75 eq.). The reaction was heated to reflux overnight. Upon concentration a precipitate forms, which was filtered, and used directly. Alternatively, the crude may be purified via flash chromatography on silica gel, eluted with ethyl acetate/hexane or acetone/hexane.
[00164] Procedure H: general procedure for the synthesis of phenylaminopyrimidines
[00165] The eneaminone (200umol) and guanidine (300umol to 500umol, 1.5 to 2.5 eq.) were dissolved in acetonitrile (200uL to 500uL).The reaction was sealed and heated to approximately 80C, overnight. The reaction was extracted with ethyl acetate and water. The organics were washed with brine, dried over sodium sulfate, filtered, and concentrated to crude. The crude was either recrystallized from ethyl acetate, ethyl acetate/hexane, ether, or ether/hexane. Otherwise, the crude was purified via flash chromatography on silica gel, eluted with ethyl acetate/hexane or ethyl acetate.
[00166] Procedure J: general procedure for the synthesis of phenylaminopyrimidines
[00167] The eneaminone (200umol) and guanidine (300umol to 500umol, 1.5 to 2.5 eq.) was dissolved in approximately 1ml of dimethylformamide (alternatively, DMSO). The reaction was sealed and heated to approximately 120C, overnight. The product can either be precipitated via addition of ethyl acetate and IN hydrochloric acid, or purified via reverse phase HPLC using a C18 column and eluted with an acetonitrile/water (with 0.1% trifluoroacetic acid v/v) gradient.
[00168] Procedure J (modified): general procedure for the synthesis of phenylaminopyrimidines
[00169] As per general procedure J except for the addition of powdered potassium carbonate (1 equivalent) or excess.
[00170] Procedure K: general procedure for the preparation of phenylaminopyrimidines
[00171] The eneaminone (400umol) and guanidine (400umol , 1 eq.) were dissolved in absolute ethanol (2ml). The reaction was sealed and heated for up to 7 days. The resultant product can either be filtered or purified via silica gel chromatography and eluted with ethyl acetate/hexanes, ethyl acetate, or acetone. Otherwise, reverse phase HPLC on C18 utilizing a gradient of acetonitrile/water with 0.1%TFA may be employed.
[00172] Procedure L: general procedure for the reduction of nitrobenzenes to anilines
[00173] The nitrobenzene (100 to 200umol) was dissolved in DMF(3ml), in a tube. To this was added methanol (1ml), iron powder (200mg to 300mg, excess), and saturated aqueous sodium bicarbonate (0.25 to 0.5 ml). The tube was sealed and heated to 96C, overnight. The reactions were cooled, filtered, and concentrated to give crude product. The products were purified on silica gel eluted with ethyl acetate/hexanes with 1% triethylamine, or via reverse phase HPLC on C18 eluted with acetonitrile/water with 0.1% TFA. [00174] Procedure M: general procedure for amide couplings from carboxylic acids [00175] The carboxylic acid (100 to 200umol) was placed in a tube with EDC (110 to 400umol, 1.1 to 2 equivalents), HOBT (40 to 200umol, 0.4 to 1 equivalent), DMF (1 to 3ml), and N-methylmorpholine (250 to 500ul). To this mixture was added desired amine or aminehydrochloride salt (300 to 600umol, 3 to 6 equivalents). The reaction was sealed and heated to 122C, overnight. The reaction was then concentrated to dryness and extracted with ethyl acetate/water. The organic layer was dried over sodium sulfate, filtered and concentrated to give crude product. The product was purified via silica gel chromatography eluted with ethyl acetate/hexanes, ethyl acetate or acetone. [00176] Procedure N: general procedure for hydrolysis of esters
[00177] The ester (100 to 200umol) was dissolved in DMF (1 to 3ml). To this was added IN sodium hydroxide (2 to 5 equivalents). The reaction was warmed to 50C several hours until complete. The reaction was quenched with IN hydrochloric acid (2 to 5 equivalents). The resulting product was filtered and dried in vacuo with warming.
Figure imgf000065_0001
Figure imgf000066_0001
[00178] "X" indicates the absence of a procedure and lower case letters indicate the use of the modified procedures. [00179] Example 2: JAK3 Inhibition Assay
[00180] Compound inhibition of JAK was assayed by the method described by G. R. Brown, et al, Bioorg. Med. Chem. Lett. 2000, vol. 10, pp 575-579 in the following manner. Into Maxisorb plates, previously coated at 4°C with Poly (Glu, Ala, Tyr) 6:3:1 then washed with phosphate buffered saline 0.05% and Tween (PBST), was added 2 μM ATP, 5 mM MgCl2, and a solution of compound in DMSO. The reaction was started with JAK enzyme and the plates incubated for 60 minutes at 30°C. The plates were then washed with PBST, 100 μL HRP-Conjugated 4G10 antibody was added, and the plate incubated for 90 minutes at 30°C. The plate was again washed with PBST, 100 μL TMB solution is added, and the plates were incubated for another 30 minutes at 30°C. Sulfuric acid (100 μL of IM) was added to stop the reaction and the plate is read at 450 nm to obtain the optical densities for analysis to determine Ki values.
[00181] The compound numbers correspond to the compound numbers in Table 1 and compounds were tested and found to inhibit JAK-3. Certain compounds described herein were shown to have KjS less than 0.1 micromolar (μM).
[00182] Example 3: CDK2 Inhibition Assay
[00183] Compounds were screened for their ability to inhibit CDK-2/Cyclin A using a standard coupled enzyme assay (Fox et al (1998) Protein Sci 7, 2249). Reactions were carried out in 100 mM HEPES pH 7.5, 10 mM MgCl2, 25 mM NaCI, 1 mM DTT and 1.5% DMSO. Final substrate concentrations in the assay were 100 μM ATP (Sigma chemicals) and 100 μM peptide (American Peptide, Sunnyvale, CA). Assays were carried out at 30°C and 25 nM CDK-2/Cyclin A. Final concentrations of the components of the coupled enzyme system were 2.5 mM phosphoenolpyruvate, 350 μM NADH, 30 μg/ml pyruvate kinase and 10 μg/ml lactate dehydrogenase.
[00184] . An assay stock buffer solution was prepared containing all of the reagents listed above, with the exception of CDK-2/Cyclin A, DTT and the test compound of interest. 56 μl of the test reaction was placed in a 384 well plate followed by addition of 1 μl of 2 mM DMSO stock containing the test compound (final compound concentration 30 μM). The plate was preincubated for -10 minutes at 30 °C and the reaction initiated by addition of 10 μl of enzyme (final concentration 25 nM). Rates of reaction were obtained using a BioRad Ultramark plate reader (Hercules, CA) over a 5 minute read time at 30°C and KiS were determined using standard methods.
[00185] The compound numbers correspond to the compound numbers in Table 1 and compounds were tested and found to inhibit CDK-2 Certain compounds described herein were shown to have KjS less than 0.1 micromolar (μM).
[00186] Example 4: JNK3 Inhibition Assays
[00187] Compounds were assayed for the inhibition of JNK3 by a spectrophotometric coupled-enzyme assay. In this assay, a fixed concentration of activated JNK3 (10 nM) was incubated with various concentrations of a potential inhibitor dissolved in DMSO for 10 minutes at 30°C in a buffer containing 0.1 M HEPES buffer, pH 7.5, containing 10 mM MgCl2, 2.5 mM phosphoenolpyruvate, 200 μM NADH, 150 μg/mL pyruvate kinase, 50 μg/mL lactate dehydrogenase, and 200 μM EGF receptor peptide. The reaction was initiated by the addition of 10 μM ATP and the assay plate is inserted into the spectrophotometer's assay plate compartment that was maintained at 30°C. The decrease of absorbance at 340 nm was monitored as a function of time. The rate data as a function of inhibitor concentration was fitted to competitive inhibition kinetic model to determine the Kj.
[00188] The compound numbers correspond to the compound numbers in Table 1 and compounds were tested and found to inhibit JNK-3. Certain compounds described herein were shown to have KjS less than 0.1 micromolar (μM).
[00189] Example 6: Syk Inhibition Assay
[00190] Compounds were screened for their ability to inhibit Syk using a standard coupled enzyme assay (Fox et al (1998) Protein Sci 7, 2249). Reactions were carried out in 100 mM HEPES pH 7.5, 10 mM MgCl2, 25 mM NaCI, 1 mM DTT and 1.5% DMSO. Final substrate concentrations in the assay were 200 μM ATP (Sigma chemical Co.) and 4 μM poly Gly-Tyr peptide (Sigma Chemical Co.). Assays were carried out at 30 °C and 200 nM Syk. Final concentrations of the components of the coupled enzyme system were 2.5 mM phosphoenolpyruvate, 300 μM NADH, 30 μg/ml pyruvate kinase and 10 μg/ml lactate dehydrogenase.
[00191] An assay stock buffer solution was prepared containing all of the reagents listed above, with the exception of Syk, DTT and the test compound of interest. 56 μl of the test reaction was placed in a 96 well plate followed by the addition of 1 μl of 2 mM DMSO stock containing the test compound (final compound concentration 30 μM). The plate was pre- incubated for ~10 minutes at 30 °C and the reaction initiated by the addition of 10 μl of enzyme (final concentration 25 nM). Rates of reaction were obtained using a BioRad Ultramark plate reader (Hercules, CA) over a 5 minute read time at 30°C, and KjS were determined using standard methods.
[00192] The compound numbers correspond to the compound numbers in Table 1 and were found to inhibit Syk. Certain compounds described herein were shown to have KjS less than 0.1 micromolar (μM).
[00193] Example 6: GSK-3 Inhibition Assay
[00194] Compounds were screened for their ability to inhibit GSK3-β (AA 1-420) activity using a standard coupled enzyme system (Fox et al. (1998) Protein Sci. 7, 2249). Reactions were carried out in a solution containing 100 mM HEPES (pH 7.5), 10 mM MgCl2, 25 mM NaCI, 300 μM NADH, 1 mM DTT and 1.5% DMSO. Final substrate concentrations in the assay were 10 μM ATP (Sigma Chemicals, St Louis, MO) and 300 μM peptide (American Peptide, Sunnyvale, CA). Reactions were carried out at 30°C and 60 nM GSK-3β. Final concentrations of the components of the coupled enzyme system were 2.5 mM phosphoenolpyruvate, 300 μM NADH, 30 μg/ml pyruvate kinase and 10 μg/ml lactate dehydrogenase.
[00195] An assay stock buffer solution was prepared containing all of the reagents listed above with the exception of ATP and the test compound of interest. 59 μl of the test reaction was placed in a 96 well H-diameter plate (Corning, Coming, NY) then treated with 1 μl of a 2 mM DMSO stock containing the test compound (final compound concentration 30 μM). The plate was incubated for ~ 10 minutes at 30 °C then the reaction initiated by addition of 7 μl of ATP (final concentration 10 μM). Rates of reaction were obtained using a Molecular Devices Spectramax plate reader (Sunnyvale, CA) over a 5 minute read time at 30 °C, and Kj values were determined using standard methods.
[00196] The compound numbers correspond to the compound numbers in Table 1 and were found to inhibit GSK-3. Certain compounds described herein were shown to have KiS less than 0.1 micromolar (μM).

Claims

1. A compound of formula I:
Figure imgf000070_0001
or a pharmaceutically acceptable salt thereof, wherein:
R1 is Q-Ar1, wherein Q is a bond or is a .2 alkylidene chain wherein one methylene unit of Q is optionally replaced by O, NR, NRCO, NRCONR, NRCO2, CO, CO2, CONR, OCONR, SO2, SO2NR, NRSO2, NRSO2NR, COCO, or COCH2CO;
Ar1 is a 5-7 membered saturated, partially unsaturated, or fully unsaturated monocyclic ring having 0-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or an 8-12 membered saturated, partially unsaturated, or fully unsaturated bicyclic ring system having 0-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; wherein Ar1 is optionally substituted with q independent occurrences of Z-Rz; wherein q is 0- 5, Z is a bond or is a Cι-C6 alkylidene chain wherein up to two methylene units of Z are optionally and independently replaced by CO, CO2, COCO, CONR, OCONR, NRNR, NRNRCO, NRCO, NRCO2, NRCONR, SO, SO2, NRSO2, SO2NR, NRSO2NR, O, S, or NR; and each occurrence of R is independently selected from R', halogen, NO2, CN, OR', SR', N(R')2, NR'COR', NR'CON(R')2, NR'CO2R', COR', CO2R', OCOR', CON(R')2, OCON(R')2, SOR', SO2R', SO2N(R') , NR'SO2R', NR'SO2N(R')2, COCOR', or COCH2COR'; each occurrence of R is independently hydrogen or an optionally substituted Cι.6 aliphatic group; and each occurrence of R is independently hydrogen or an optionally substituted Cι_6 aliphatic group, a 3-8-membered saturated, partially unsaturated, or fully unsaturated monocyclic ring having 0-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or an 8-12 membered saturated, partially unsaturated, or fully unsaturated bicyclic ring system having 0-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or R and R , two occurrences of R, or two occurrences of R , are taken together with the atom(s) to which they are bound to form an optionally substituted 3-12 membered saturated, partially unsaturated, or fully unsaturated monocyclic or bicyclic ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur; z s N or CH; Z7 is N or C-URY;
T and U are each independently a bond or a saturated or unsaturated
Figure imgf000071_0001
alkylidene chain, wherein up to two methylene units of the chain are optionally and independently replaced by CO, CO2, COCO, CONR, OCONR, NRNR, NRNRCO, NRCO, NRCO2, NRCONR, SO, SO2, NRSO2, SO2NR, NRSO2NR, O, S, or NR;
Rx and Rγ are each independently halogen, CN, NO , or R';
Z2, Z5 and Z6 are each independently N or CH, provided that no more than two of Z2, Z5, and Z6 are N;
Z3 is CR3;
Z4 is CR4; wherein one of R3 or R4 is Ru, and the other of R3 or R4 is RV1, wherein: Ru is (CH2)tCN, (CH2)tNO2, (CH2)tN(R)2, (CH2)tNRC(O)R, (CH2)tCON(R)2, (CH2)tCOOR, (CH2)tSO2N(R)2, (CH2)tNRSO2R, (CH2)tNRCON(R)2, (CH2)tNRSO2N(R)2, (CH2)tCOCOR, (CH2)tAr2, wherein t is 0, 1, or 2, and Ar2 is an optionally substituted 5-7 membered saturated, partially unsaturated, or fully unsaturated monocyclic ring having 0-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur; and RV1 is OR'.
2. The compound according to claim 1, wherein Z1 is N and the compound has the structure II.
Figure imgf000071_0002
II
3. The compound according to claim 1, wherein Z is CH and the compound has the structure III:
Figure imgf000072_0001
III.
4. The compound according to claim 1, wherein R1 is an optionally substituted phenyl, cyclohexyl, cyclopentyl, pyridyl, naphthyl, morpholino, piperazinyl, or piperidinyl ring.
5. The compound according to claim 1, wherein R1 is an optionally substituted group selected from phenyl, cyclohexyl, or pyridyl.
6. The compound according to claim 1, wherein R1 is optionally substituted phenyl.
7. The compound according to claim 1, wherein each independent occurrence of ZRZ is halogen, R', CN, NO2, -N(R)(R'), -OR', -SR', -S(O)2N(R)(R'), CO(CMalkyl), -NRSO2R', COO CMalkyl).
8. The compoxmd according to claim 1, wherein q is 1 or 2 and each independent occurrence of ZRZ is F, Cl, Br, COO(C alkyl), Cι_4alkyl, CN, NO2, -NH2, -OH, CMalkoxy, -S(O) NH2, or an optionally substituted benzyloxy, phenyloxy, or phenyl group.
9. The compound according to claim 1, wherein TRX and URY are each independently selected from hydrogen, halogen, NO , CN, OR, SR or N(R)2, or Cι-4aliphatic optionally substituted with oxo, OR, SR, N(R)2, halogen, NO2 or CN.
10. The compound according to claim 1, wherein TRX and URY groups are each independently hydrogen, Me, OH, OMe or N(R)2.
11. The compound according to claim 1, wherein (T)mRx and (U)nRY are each hydrogen.
12. The compound according to claim 1, wherein ring B is selected from one of the i-viii:
Figure imgf000073_0001
n in
Figure imgf000073_0002
iv v VI
Figure imgf000073_0003
Figure imgf000073_0004
13. The compound according to claim 1, having one of the structures:
Figure imgf000073_0005
II-A III-A
Figure imgf000073_0006
II-B III-B
Figure imgf000074_0001
II-C III-C
Figure imgf000074_0002
II-D III-D
Figure imgf000074_0003
II-E III-E
Figure imgf000074_0004
II-F III-F
Figure imgf000074_0005
II-G III-G
Figure imgf000075_0001
II-H III-H
Figure imgf000075_0002
II-I III-I
Figure imgf000075_0003
II-J III-J
Figure imgf000075_0004
II-K III-K
Figure imgf000075_0005
II-L III-L
Figure imgf000076_0001
II-M III-M
Figure imgf000076_0002
II-N III-N
Figure imgf000076_0003
II-O mo
Figure imgf000076_0004
IIP III-P
Figure imgf000077_0001
II-Q III-Q
Figure imgf000077_0002
II-R III-R
Figure imgf000077_0003
II-S III-S
Figure imgf000077_0004
II-T III-T
Figure imgf000077_0005
II-U III-U
Figure imgf000078_0001
II-V πi-v
Figure imgf000078_0002
II-W III-W
Figure imgf000078_0003
II-X III-X
14. The compound according to claim 13, wherein compound variables are selected from one or more, or all of the following groups: i) R1 is selected from an optionally substituted group selected from phenyl, cyclohexyl, cyclopentyl, pyridyl, morpholino, piperazinyl, or piperidinyl; ii) R1 is an optionally substituted group selected from phenyl, cyclohexyl, or pyridyl; iii) R1 is optionally substituted phenyl; iv) Ar1 is substituted with up to five occurrences of ZR , and ZRZ groups are selected from halogen, R', CN, NO2, -N(R)(R'), -OR', -SR', -S(O)2N(R)(R'), CO(CMalkyl), - NRSO2R', COO(CMalkyl). v) q is 1 and ZRZ is F, Cl, Br, COO^^alkyl), CMalkyl, CN, NO2, -NH2, -OH, . 4alkoxy, -S(O)2NH2, or an optionally substituted benzyloxy, phenyloxy, or phenyl group; vi) q is 1, and ZR ,Z i •s i •n t ,ιhe met ,a or para posi •t.i:on and j Z rr-RnZ is F, Cl, Br, benzyloxy, phenyl, phenyloxy, COO(Cι-4alkyl), -NH2, -OH, C^alkoxy, or -S(O)2NH2; vii) TRX and URY are selected from hydrogen, halogen, NO2, CN, OR, SR or N(R)2, or Ci. aliphatic optionally substituted with oxo, OR, SR, N(R)2, halogen, NO2 or CN; viii) TRX and URY groups are selected from hydrogen, Me, OH, OMe or N(R)2; ix) TR and UR are each hydrogen; x) Z2 and Z6 are each CH; xi) t is 0; xii) t is 1; and xiii) Ar2 is an optionally substituted tetrazole, triazole, oxazole, thiazole, thiadiazole, oxadiazole or pyridyl group.
15. The compound according to claim 1, wherein compounds have one of the structures:
Figure imgf000079_0001
II-B-(i) III-B-(i)
Figure imgf000079_0002
II-C-(i) III-C-(i)
Figure imgf000080_0001
II-E-(i) III-E-(i)
Figure imgf000080_0002
II-F-(i) III-F-(i)
Figure imgf000080_0003
II-G-(i) III-G-(i)
Figure imgf000081_0001
II-J-(i) III-J-(i)
Figure imgf000081_0002
II-K-(i) III-K-(i)
Figure imgf000081_0003
Figure imgf000082_0001
II-N-(i) III-N-(i)
Figure imgf000082_0002
H-O-(i) III-O-(i)
Figure imgf000082_0003
Figure imgf000083_0001
II-R-(i) III-R-(i)
Figure imgf000083_0002
II-S-(i) III-S-(i)
Figure imgf000083_0003
II-U-(i) m-u-(i)
Figure imgf000084_0001
II-V-(i) III-V-(i)
Figure imgf000084_0002
II-W-(i) III-W-(i)
Figure imgf000084_0003
16. The compound according to claim 1, where ring A is a pyrimidine; UR , and TR are each hydrogen, and the compounds have the general structures:
Figure imgf000084_0004
II-A-(i)-a II-B-(i)-a
Figure imgf000085_0001
II-C-(i)- II-D-(i)-a
Figure imgf000085_0002
II-E-(i)-a II-F-(i)-a
Figure imgf000085_0003
Figure imgf000085_0004
II-I-(i)-a II-J-(i)-
Figure imgf000085_0005
II-K-(i)- II-L-(i)-a
Figure imgf000086_0001
II-M-(i)-a II-N-(i)-a
Figure imgf000086_0002
II-O-(i)-a II-P-(i)-
Figure imgf000086_0003
II-S-(i)-a II-T-(i)-a
Figure imgf000087_0001
II-U-(i)-a II-V-(i)-a
Figure imgf000087_0002
II-W-(i)-a II-X-(i)-a.
17. The compound according to claim 16, wherein: a. q is 0, 1, or 2, and ZRZ is halogen, R', CN, NO2, -N(R)(R'), -OR', -SR' S(O)2N(R)(R'), CO(CMalkyl), -NRSO2R', COO(C alkyl); b. t is O; c. R is optionally substituted C^alkyl or hydrogen; and d. R' is optionally substituted Cι_6alkyl or hydrogen.
18. The compound according to claim 1, selected from one of the structures:
Figure imgf000087_0003
Figure imgf000087_0004
II-4 II- 5 II- 6
Figure imgf000088_0001
II- 7 II-8 II- 9
Figure imgf000088_0002
π-10 π-11 π-12
Figure imgf000088_0003
11-13 π-14 π-15
Figure imgf000088_0004
π-i6 11-17 π-18
Figure imgf000088_0005
π-19 11-20 11-21
Figure imgf000089_0001
11-22 11-23 11-24
Figure imgf000089_0002
11-25 π-26 11-27
Figure imgf000089_0003
11-28 11-29 11-30
Figure imgf000089_0004
11-31 11-32 11-33
Figure imgf000089_0005
Figure imgf000090_0001
11-36 11-37 π-38
Figure imgf000090_0002
π-39 π-40 11-41
Figure imgf000090_0003
π-42 11-43 π-44
Figure imgf000090_0004
π-48 11-49 π-50
Figure imgf000091_0001
11-51 π-52 11-53
Figure imgf000091_0002
11-60 π-61 π-62
Figure imgf000091_0003
Figure imgf000092_0001
11-66 11-67 11-68
Figure imgf000092_0002
π-69 π-70 ii-7i
Figure imgf000092_0003
11-72 11-73 π-74
Figure imgf000092_0004
π-75 π-76 π-77
Figure imgf000092_0005
π-78 π-79 π-80
Figure imgf000093_0001
-81 -82 11-83
Figure imgf000093_0002
11-84 11-85 π-86
Figure imgf000093_0003
π-87 11-88 π-89
Figure imgf000093_0004
11-90 π-9i 11-92
Figure imgf000094_0001
π-93 11-94 11-95
Figure imgf000094_0002
π-96 π-97 π-98
Figure imgf000094_0003
11-99 II-100 II-101
Figure imgf000094_0004
Figure imgf000094_0005
Figure imgf000095_0001
π-108 π-109 11-110
Figure imgf000095_0002
11-111 π-112 π-113
Figure imgf000095_0003
π-ιi4 π-ιi5 π-116
Figure imgf000095_0004
π-117 π-118 π-119
Figure imgf000095_0005
π-120 π-121 11-122
Figure imgf000096_0001
11-123 II-124 11-125
Figure imgf000096_0002
11-126 11-127 11-128
Figure imgf000096_0003
II-129 π-130 π-131
Figure imgf000096_0004
II-132 11-133 11-134
Figure imgf000096_0005
11-135 11-136 11-137
Figure imgf000097_0001
π-141 11-142 II-143
Figure imgf000097_0002
II-144 11-145 11-146
Figure imgf000097_0003
11-147 11-148 II-149
Figure imgf000097_0004
-150 11-151 11-152
Figure imgf000098_0001
-153 -154 11-155
Figure imgf000098_0002
Figure imgf000098_0003
Figure imgf000098_0004
Figure imgf000098_0005
Figure imgf000098_0006
Figure imgf000098_0007
Figure imgf000099_0001
Figure imgf000099_0002
Figure imgf000099_0003
111-22 111-23 111-24
Figure imgf000100_0001
111-25 111-26 111-27
Figure imgf000100_0002
111-28 111-29 111-30
Figure imgf000100_0003
πι-31 111-32 111-33 or III-34
19. A pharmaceutical composition comprising a compound according to claim 1, and a pharmaceutically acceptable carrier, adjuvant, or vehicle.
20. The composition according to claim 25, further comprising an additional therapeutic agent selected from a chemotherapeutic or anti-proliferative agent, a treatment for Alzheimer's Disease, a treatment for Parkinson's Disease, an agent for treating Multiple Sclerosis (MS), a treatment for asthma, an agent for treating schizophrenia, an anti- inflammatory agent, an immunomodulatory or immunosuppressive agent, a neurofrophic factor, an agent for treating cardiovascular disease, an agent for treating destructive bone disorders, an agent for treating liver disease, an agent for freating a blood disorder, or an agent for treating an immunodeficiency disorder.
21. A method of inhibiting JAK-3, JNK-3, CDK-2, SYK, GSK-3 kinase activity in
(a) a patient; or
(b) a biological sample; which method comprises administering to said patient, or contacting said biological sample with a compound of claim 1 or a composition of claim 19.
22. A method of treating or lessening the severity of a disease or disorder selected from an immune response, an autoimmune disease, a neurodegenerative disorder, or a solid or hematologic malignancy comprising administering to a subject in need thereof a compound of claim 1 or a composition of claim 24.
23. The method of claim 22, wherein the disease or disorder is selected from an allergic or type I hypersensitivity reaction, asthma, transplant rejection, graft versus host disease, rheumatoid arthritis, amyotrophic lateral sclerosis, multiple sclerosis, Familial amyotrophic lateral sclerosis (FALS), leukemia, or lymphoma.
24. The method of claim 22, comprising the further step of administering to said patient an additional therapeutic agent selected from a chemotherapeutic or anti-proliferative agent, a treatment for Alzheimer's Disease, a treatment for Parkinson's Disease, an agent for treating Multiple Sclerosis (MS), a freatment for asthma, an agent for treating schizophrenia, an anti- inflammatory agent, an immunomodulatory or immunosuppressive agent, a neurofrophic factor, an agent for treating cardiovascular disease, an agent for treating destructive bone disorders, an agent for treating liver disease, an agent for treating a blood disorder, or an agent for treating an immunodeficiency disorder, wherein: said additional therapeutic agent is appropriate for the disease being treated; and said additional therapeutic agent is administered together with said composition as a single dosage form or separately from said composition as part of a multiple dosage form.
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