WO2004029284A3 - Efficient generation of stable expression cell lines through the use of scorable homeostatic reporter genes - Google Patents
Efficient generation of stable expression cell lines through the use of scorable homeostatic reporter genes Download PDFInfo
- Publication number
- WO2004029284A3 WO2004029284A3 PCT/US2003/031311 US0331311W WO2004029284A3 WO 2004029284 A3 WO2004029284 A3 WO 2004029284A3 US 0331311 W US0331311 W US 0331311W WO 2004029284 A3 WO2004029284 A3 WO 2004029284A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- cell
- vector
- scorable
- homeostatic
- methods
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
- C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biophysics (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Mycology (AREA)
- Medicinal Chemistry (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AU2003283995A AU2003283995A1 (en) | 2002-09-30 | 2003-09-30 | Efficient generation of stable expression cell lines through the use of scorable homeostatic reporter genes |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US41521602P | 2002-09-30 | 2002-09-30 | |
US60/415,216 | 2002-09-30 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2004029284A2 WO2004029284A2 (en) | 2004-04-08 |
WO2004029284A3 true WO2004029284A3 (en) | 2005-05-12 |
Family
ID=32043428
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2003/031311 WO2004029284A2 (en) | 2002-09-30 | 2003-09-30 | Efficient generation of stable expression cell lines through the use of scorable homeostatic reporter genes |
Country Status (3)
Country | Link |
---|---|
US (2) | US20040115814A1 (en) |
AU (1) | AU2003283995A1 (en) |
WO (1) | WO2004029284A2 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR101555658B1 (en) | 2007-05-25 | 2015-09-24 | 심포젠 에이/에스 | Method for manufacturing a recombinant polyclonal protein |
Families Citing this family (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
SI1583830T1 (en) | 2003-01-07 | 2006-12-31 | Symphogen As | Method for manufacturing recombinant polyclonal proteins |
RS20060266A (en) * | 2003-10-14 | 2008-09-29 | Biogen Idec Ma Inc., | Flp-mediated recombination |
JP2011517317A (en) * | 2008-02-28 | 2011-06-02 | ライフ テクノロジーズ コーポレーション | Fluorescence polarization hERG assay |
EP2105505A1 (en) | 2008-03-28 | 2009-09-30 | Celonic AG | Methods and materials for the reproducible generation of high producer cell lines for recombinant proteins |
US10412788B2 (en) | 2008-06-13 | 2019-09-10 | Lg Chem, Ltd. | Heating element and manufacturing method thereof |
GB0810924D0 (en) * | 2008-06-14 | 2008-07-23 | Rowett Res Inst | In vivo transgenics |
US20130071881A1 (en) * | 2009-11-19 | 2013-03-21 | Immuno Tec Laboratory Co. Ltd | Methods for producing antibody-producing cells that produce desired polypeptides |
US20130244907A1 (en) * | 2010-11-18 | 2013-09-19 | National University Corporation Okayama University | Method for preparing b cell which produces human-type antibody |
AU2013318147B2 (en) | 2012-09-19 | 2018-01-04 | Abbvie Biotherapeutics Inc. | Methods for identifying antibodies with reduced immunogenicity |
US9910038B2 (en) | 2012-11-30 | 2018-03-06 | Larix Bioscience, Llc | Cell line screening method |
SG10201601929YA (en) * | 2013-03-15 | 2016-04-28 | Promega Corp | Activation Of Bioluminescence By Structural Complementation |
JP6788573B6 (en) | 2014-04-10 | 2020-12-16 | シアトル チルドレンズ ホスピタル, ディービーエー シアトル チルドレンズ リサーチ インスティテュート | Production of genetically modified T cells by Sleeping Beauty transposon in combination with selection by methotrexate |
GB201411344D0 (en) * | 2014-06-26 | 2014-08-13 | Univ Leicester | Cloning |
AU2016306209B2 (en) | 2015-08-07 | 2023-07-06 | Seattle Children's Hospital (dba Seattle Children's Research Institute) | Bispecific CAR T-cells for solid tumor targeting |
GB201703418D0 (en) | 2017-03-03 | 2017-04-19 | Ge Healthcare Bio Sciences Ab | Method for cell line development |
GB201703417D0 (en) | 2017-03-03 | 2017-04-19 | Ge Healthcare Bio Sciences Ab | Method for cell line development |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4816567A (en) * | 1983-04-08 | 1989-03-28 | Genentech, Inc. | Recombinant immunoglobin preparations |
US6091001A (en) * | 1995-03-29 | 2000-07-18 | Abgenix, Inc. | Production of antibodies using Cre-mediated site-specific recombination |
US6143557A (en) * | 1995-06-07 | 2000-11-07 | Life Technologies, Inc. | Recombination cloning using engineered recombination sites |
US20020007051A1 (en) * | 1999-12-10 | 2002-01-17 | David Cheo | Use of multiple recombination sites with unique specificity in recombinational cloning |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5202238A (en) * | 1987-10-27 | 1993-04-13 | Oncogen | Production of chimeric antibodies by homologous recombination |
WO1992015694A1 (en) * | 1991-03-08 | 1992-09-17 | The Salk Institute For Biological Studies | Flp-mediated gene modification in mammalian cells, and compositions and cells useful therefor |
BR0112633A (en) * | 2000-07-21 | 2003-09-16 | Us Agriculture | Method for replacing, translocating and stacking DNA in eukaryotic genomes |
-
2003
- 2003-09-30 WO PCT/US2003/031311 patent/WO2004029284A2/en not_active Application Discontinuation
- 2003-09-30 US US10/676,476 patent/US20040115814A1/en not_active Abandoned
- 2003-09-30 AU AU2003283995A patent/AU2003283995A1/en not_active Abandoned
-
2006
- 2006-08-23 US US11/509,177 patent/US20060286671A1/en not_active Abandoned
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4816567A (en) * | 1983-04-08 | 1989-03-28 | Genentech, Inc. | Recombinant immunoglobin preparations |
US6091001A (en) * | 1995-03-29 | 2000-07-18 | Abgenix, Inc. | Production of antibodies using Cre-mediated site-specific recombination |
US6143557A (en) * | 1995-06-07 | 2000-11-07 | Life Technologies, Inc. | Recombination cloning using engineered recombination sites |
US20020007051A1 (en) * | 1999-12-10 | 2002-01-17 | David Cheo | Use of multiple recombination sites with unique specificity in recombinational cloning |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR101555658B1 (en) | 2007-05-25 | 2015-09-24 | 심포젠 에이/에스 | Method for manufacturing a recombinant polyclonal protein |
Also Published As
Publication number | Publication date |
---|---|
US20040115814A1 (en) | 2004-06-17 |
WO2004029284A2 (en) | 2004-04-08 |
US20060286671A1 (en) | 2006-12-21 |
AU2003283995A1 (en) | 2004-04-19 |
AU2003283995A8 (en) | 2004-04-19 |
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